Dissertationen zum Thema „Shock Mobility“

Social mobility is often viewed as a way to alleviate poverty and create equality; it represents the basis upon which the United States is viewed as a meritocratic nation of opportunity. Missing from this persistent narrative, however, is analysis of the actual experience of social mobility. This qualitative study explores the narratives of individuals as they reflect on their experiences of upward mobility through education. Data include in-depth interviews with 25 individuals with an advanced degree whose parents did not attend college, and 10 individuals who have an advanced degree similar to their parents. This study considers three dimensions of cultural capital—embodied cultural capital associated with how individuals present themselves, linguistic cultural capital associated with how individuals speak and communicate and cultural capital related to taste, beliefs and knowledge, associated with individual’s leisure time choices, food and drink preferences and beliefs about the world. Across data, mobile individuals express the expectation or need to take on the cultural practices and behavior of their new class context. Data suggest that the process through which upwardly mobile individuals experience shifts in culture is more complex than currently conceived. In addition, these changes in culture can lead to internal conflict and difficulty in connection with families of origin; representing the potential costs of upward mobility. Implications include an amendment to cultural mobility research and to current strategies in urban education which position cultural capital as a character trait that can be learned or taken up by individuals.

Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.
Title from electronic title page (viewed Apr. 25, 2008). "... in partial fulfillment of the requirements for the degree of PhD in the School of Medicine Human Movement Science Curriculum." Discipline: Human Movement Science; Department/School: Medicine.

When the COVID-19 pandemic hit Europe in the beginning of 2020, a majority of countries reacted by closing their state borders. By drawing on concepts of border studies and border regional resilience, this thesis aims to examine the impact of the (im)mobility shock caused by the pandemic on two border twin cities in Europe. Through a qualitative approach, conducting semi-structured expert interviews and content analyses, the thesis attempts to identify (1) Which factors facilitated/obstructed the coping/adapting strategies to the problems caused by the pandemic induced state border closures; and (2) Which long-term effects the COVID-19 crisis will have on the future development of the border twin cities and whether their development path will transform into a more resilient one. The main finding of this thesis is that the crisis management on the local level was considerably limited by the restrictions enacted at the national level. To be better prepared for similar shocks, the border twin cities have already made plans based on their experience. However, it remains to be seen whether these strategies can contribute to the resilience of the cities. Nevertheless, coordination between the local, regional and national level is essential to plan and establish resilient border twin cities.

Notre recherche s'intéresse à la mobilité étudiante libyenne. Les particularités du public concerné ainsi que les objectifs de leur séjour en France différencient à plusieurs égards cette mobilité d'autres formes de mobilité académique. En effet, notre échantillon est constitué de professeurs (dans différents domaines) ayant repris des études et qui sont, en partie, des non-spécialistes en langue française, donc sans connaissance préalable de cette langue. Un certain nombre de ces étudiants était accompagné de leur famille. Ces faits constituent les spécificités les plus saillantes de l'échantillon d'enquête. L'étude s'intéresse, au moyen d'une approche quantitative, au parcours de ces étudiants « particuliers ». Notre recherche veut mettre en lumière les manières dont ces étudiants, qui ont longtemps vécu dans un système fermé où ils étaient, entre autres, exposés à la diffusion d'images négatives sur l'Occident ont fait face à une altérité linguistique et culturelle très différente de leur milieu d'origine. Elle interroge l'impact du choc culturel, les stratégies d'adaptation à des pratiques sociales qui ne leur sont absolument pas familières et leur disposition à l'ouverture. Une importance particulière est accordée à l'influence de la religion, base de leur culture d'origine, sur la perception de la différence. Nous montrons que la mise en perspective de deux univers opposés induira des questionnements sur l'autre et sur soi auxquels les étudiants répondront par une forte résistance au milieu d'accueil, nourrie par un bouclier identitaire omniprésent, même si l'expérience a permis à certains d'acquérir un regard plus large sur la différence et sur l'échange avec l'autre différent. Par ailleurs, l'étude pointe les failles du système éducatif libyen et de la politique éducative libyenne en général, caractérisés par un manque de vision globale et de décisions aléatoires. Par ses résultats, l'étude espère contribuer à améliorer la compréhension des problèmes spécifiques auxquels ces étudiants sont confrontés et qui rendent indispensables une préparation, un accompagnement ainsi qu'un retour réflexif sur l'expérience
Our research deals with the Libyan students’ mobility, but this study differs from the usual student studies due to the nature of its sample and the objectives of this mobility. Indeed, our sample is composed of professors (in different areas) who have resumed studies, a growing number of non-specialists in French who have never studied this language before, and finally the very nature of this mobility is intriguing because these professors have moved with their families. We are talking about the specificities of the Libyan students’ mobility. Through a thorough study, the work focuses on the journey of these students 'special needs'. Indeed this work is to measure the extent of the culture shock experienced by these very students. The analysis shed light on the dynamics of their representations. The encounter with other students revealed our own students many realities about themselves during their time at universities. Our students who also have developed strong resistance home about Western identities nourished by an omnipresent identity shield face challenges and have to debunk some misinterpretations. Although the factors influencing on the social background are important, we were able to identify the most important ones for our respondents. The study also emphasizes on the flaws of the Libyan education system, and the Libyan educational policies in general, characterized by a lack of global vision and partial decision making. The result of this study can contribute to better preparation for Libyan students who want to study abroad and also to suggest that more intercultural awareness must be taken into consideration when it comes to students’ mobility

The following is a study of the impact of migration on unemployment rates for natives in Sweden, on municipal level. A cross sectional data set has been analyzed using multiple linear regressions. The regression analysis has searched for the impact on the unemployment rates among natives of the size of the share of migrants in the municipalities as well as of the change in the size of the share of migrants during a time span of 13 years. The results show that migration has small or non-existent impact on the unemployment rates of natives. The results vary depending on the period being investigated and also depending on the characteristics of the municipality that is investigated. When dividing the municipalities into three categories (city, urban and rural municipalities) significant impact of migration on native’s unemployment rates is to be found in city and urban municipalities, but not in rural. The results also indicate that the most significant impact is to be found in the present period of time, while in the long term the impact diminishes to become less significant or not significant at all. 10% migrants in a city municipality in 2015 increased natives’ unemployment level that same year by 0.4 percentage units. More rapid increases of the share of migrants in the labor force have more impact as well. A municipality were the share of migrants grew with 1 percentage unit between 2003 and 2015, had 0.1 percentage unit higher unemployment rate for natives in 2015. This study’s results follow the pattern from earlier studies in the field, that since the 1990’s have shown similar effects when measuring different countries on different continents – sometimes the effect has been significant, sometimes not, and when significant the impact has been rather small, often clustering around zero.

Ce travail s’inscrit dans le projet de développement des réservoirs essence pour voitures à partir des formulations Polyamide 6,6 (PA6,6). Le PA6,6 est un bon candidat pour des applications techniques car il possède des bonnes propriétés thermomécaniques rapportés à son poids. Ces formulations doivent montrer des bonnes propriétés barrière aux essences classiques, aux biofuels (essence contenant de l’éthanol) et aussi à l’eau qui est présente dans l’atmosphère, tout en ayant une bonne tenue aux chocs.Les enjeux scientifiques sont la meilleure compréhension des interactions entre le PA6,6 et des solvants purs de polarités et tailles différentes mais aussi des mélanges ternaires composés d’éthanol, toluène et isooctane à différentes concentrations et qui modélisent les biofuels. On veut aussi étudier l’effet de ces solvants sur la température de transition vitreuse Tg qui est la signature de la mobilité moléculaire de la phase amorphe. Ensuite, l’effet de l’additivation ou de l’ajout des co-monomères sur les propriétés barrière et mécaniques, notamment les propriétés choc, a été étudié. Dans ce travail nous avons pu décrire comment la nature d’un solvant conditionne sa sorption et son effet plastifiant dans le PA6,6. On a aussi observé que la nature et les interactions interchaînes de la matrice polymère conditionnent aussi la prise en solvants. De plus, une approche thermodynamique a été proposée pour étudier et comprendre la sorption de mélanges ternaires éthanol-toluène-isooctane et leur effet plastifiant dans le PA6,6. On a aussi montré que les propriétés mécaniques du PA6,6 dépendent ou sont liées à l’état de mobilité moléculaire de la phase amorphe de ce polymère
This work forms part of a project aimed to develop Polyamide 6,6(PA6,6)-based automotive fuel tanks. PA6,6 is a good candidate for technical applications since it possesses good thermo-mechanical properties compared to its weight. These formulations must exhibit good impermeable properties to classic gasoline, to biofuels (gasoline containing ethanol), and to water which is found in the atmosphere, while keeping good impact behavior.The scientific issues are the better understanding of PA6,6-solvent interactions in the case of a series of solvents having different sizes and polarities and also for a set of ternary mixtures composed of ethanol, toluene, and isooctane at various concentrations that model biofuels. The effect of these solvents on the glass transition temperature Tg (molecular mobility signature) was assessed. The effect of additivating or chemically-modifying PA6,6 on the impact and barrier properties of the polymer was studied.In this work, it was described how the nature of a solvent conditions its sorption and its plasticizing effect on PA6,6. It was also observed that the PA6,6 inter-chain interactions also condition the solvent sorption. Moreover, a thermodynamic approach describing the sorption and plasticizing effects of ternary mixtures in PA6,6 was considered and developed. It was also observed that PA6,6 mechanical properties depend or are related to the amorphous phase molecular mobility state

This work forms part of a project aimed to develop Polyamide 6,6(PA6,6)-based automotive fuel tanks. PA6,6 is a good candidate for technical applications since it possesses good thermo-mechanical properties compared to its weight. These formulations must exhibit good impermeable properties to classic gasoline, to biofuels (gasoline containing ethanol), and to water which is found in the atmosphere, while keeping good impact behavior.The scientific issues are the better understanding of PA6,6-solvent interactions in the case of a series of solvents having different sizes and polarities and also for a set of ternary mixtures composed of ethanol, toluene, and isooctane at various concentrations that model biofuels. The effect of these solvents on the glass transition temperature Tg (molecular mobility signature) was assessed. The effect of additivating or chemically-modifying PA6,6 on the impact and barrier properties of the polymer was studied.In this work, it was described how the nature of a solvent conditions its sorption and its plasticizing effect on PA6,6. It was also observed that the PA6,6 inter-chain interactions also condition the solvent sorption. Moreover, a thermodynamic approach describing the sorption and plasticizing effects of ternary mixtures in PA6,6 was considered and developed. It was also observed that PA6,6 mechanical properties depend or are related to the amorphous phase molecular mobility state.

This thesis examines the representation of temporary migrants in the OCVID-19 pandemic in New Zealand. The aim of the study is to understand how the pandemic effected this early decision making by the New Zealand Parliament in correlation with migration, more specific, the temporary migrants who are stuck within New Zealands borders. These issues can be linked with shock mobility, more specific, with immobility within shock. The material is analysed with the WPR method. The study concludes that there is a changing discourse and a changing representation of the temporary migrants. Where at first the New Zealand citizens have to be protected, later on in the discourse the migrants need to be helped and it is followed by financial gain that the temporary migrants bring with their labour and spending. The findings create a contribution in the field of shock mobility, with a focus on the immobility within shock mobility.

This study responds to the need for more research around (im)mobility decision-making to better support people facing environmental shocks and climatic changes. The concept of Trapped Populations, first appeared with the release of the 2011 Foresight report yielding repeated use in environmental migration studies and to a more limited extent policy. Although a seemingly straightforward concept, referring to people's inability to move away from environmental high-risk areas despite a desire to do so, the underlying reasons for someone's immobility can be profoundly complex. The empirical literature body referring to ‘trapped' populations has similarly taken a fairly simple and narrow economic explanatory approach. A more comprehensive understanding around how immobility is narrated in academia, and how people's cultural, social and psychological background in Bangladesh influences their (im)mobility, can provide crucial research insights. To better protect and support people living with environmental shocks and changes worldwide we need to build robust and well-informed policy frameworks To achieve this, a set of discourse analyses were carried out. Firstly, a textual Critical Discourse Analysis (CDA) reviewed how ‘trapped' has been framed within academia. Secondly, a Foucauldian inspired discourse analysis was performed on field data to explore how power, knowledge and and binary opposites shape and determine people's social norms in terms of their (im)mobility decision-making. These key concepts critically showcased how meaning, values and power can constrain the mobility of a social group. The analysis was carried out on a large set of field data gathered between 2014 and 2016 in Bangladesh. The data on urban immobility and rural non-evacuation behaviour was gathered through a mixed-method quant-qualitative approach that included Q-methodology, storytelling group sessions, in-depth interviews and a survey questionnaire. Other key concepts used to frame the analysis included those of subjectivity, gender, place and space. The textual discourse analysis highlighted the dangers of framing mobility or resettlement as a potential climate adaptation. Assisted migration, could for example end up disguising other hidden political and economic agendas. The research identified how the empirical notions of ‘trapped' move beyond economic immobility. People in Bangladesh described being socially, psychologically and emotionally ‘trapped'. These empirical notions are useful within the area of climate policy, as they raise questions around whether mobility in fact is the solution.

Il y a quatre chapitres dans cette thèse.Dans le premier chapitre, nous analysons les intéractions entre le marché interbancaire et le risque de défaut souverain dans un modèle d’équilibre général à deux pays, en focalisant sur la transmission de la crise financière récente et la politique monétaire non conventionnelle.Dans le deuxième chapitre, les effets de la dévaluation fiscale sur les indicateurs macroéconomiques et le bien être sont analysés en utilisant un modèle à deux pays en union monétaire o`u les variétés de biens et le commerce sont endogènes.Dans le troisième chapitre, l’impact du facteur démographique sur la croissance du secteur des services à long terme est mis en exergue.Dans le quatrième chapitre, on étudie les effets de la mobilité des travailleurs et de la mobilité du capital dans une union monétaire
This thesis studies challenges for modern developped economies, including the structural change toward services, population ageing, weak labor mobility in the EMU and unconventional monetary policies after the 2008 financial crisis. The manuscript is divided into four chapters.In the first chapter, we analyze the interaction between interbank markets and default risk using a two-country dynamic general equilibrium model, with a focus on the transmission of the recent financial crisis and unconventional monetary policies.In the second chapter, we investigate the effects of fiscal devaluations on key macroeconomic aggregates and welfare using a two-country monetary-union model with endogenous varieties and endogenous tradability.In the third chapter, we study the impact of demographic factor and the growth of service sector by using a multi-sectoral OLG model, and effectuate counterfactual experiments in which the annual growth rate of young generation is ±1pp than the actual growth rate.In the fourth chapter, we study the potential interactions between financial integration and labor mobility in a currency union facing asymmetric shocks, and simulate the impacts of 2008 financial crisis under different mobility costs

The paper written by Teece and colleagues in 1997 marked the introduction of the Dynamic Capabilities theory. Since then the framework gained massive attention by different scholars, with divergent points of view regarding the theory that intends to explain how firms can achieve sustainable competitive advantage in rapidly changing environments. In 2010, Barreto presented a more consistent view, converging all the past research, presenting Dynamic Capabilities as a four-dimension construct. My dissertation is a Teaching Case, and the focal objective is to illustrate Barreto’s view of Dynamic Capabilities in a real-life example. I decided to write my dissertation regarding Hertz Portugal, a rent-a-car company that exists in Portugal since 1959. The case explores the concept of new mobility alternatives such as car-sharing platforms present in the Portuguese market and provides insights regarding the ability of Hertz Portugal to address these changes. The case demonstrates how Hertz was able to position the company in the car-sharing market and how crucial the dimensions of dynamic capabilities are to address exogenous shocks.
O artigo escrito por Teece et al. em 1997 marcou a introdução da teoria sobre Dynamic Capabilities. Desde então o conceito ganhou imensa atenção por parte de vários autores, muitos deles com opiniões divergentes em relação a esta nova teoria que visa explicar como é que as empresas podem obter vantagem competitiva sustentável em ambientes de rápida mudança. Em 2010, Barreto apresentou uma proposta mais consistente sobre Dynamic Capabilities, convergindo estudos anteriores, apresentando por fim uma definição com quatro dimensões. A minha tese consiste num caso de estudo, e tem como objetivo principal ilustrar a proposta de Barreto num caso de vida real. Decidi escrever a minha tese sobre a Hertz Portugal, uma empresa de rent-a-car que está presente no mercado português desde 1959. O caso estuda o conceito de novas alternativas de mobilidade, como as plataformas de car-sharing presentes no mercado português, fornecendo informações sobre como é que a Hertz reagiu a estas mudanças e demonstra como a empresa se posicionou no mercado de car-sharing. Por fim o caso demonstra também como as quatro dimensões da teoria proposta por Barreto são vitais para uma empresa se adaptar a choques exógenos.

Magister Scientiae - MSc
Ischemic heart disease is a disease which is characterized by the reduced blood supply to the heart. According to WHO 2013, ischemic heart disease is one of the major causes of death globally. For this reason, it is imperative to search for methods whereby heart cells can be protected from cell death. The upregulation of heat shock proteins (Hsps) is one of the major techniques which can be used to protect the heart cells from Hsps cell death and improve the tolerance to ischemic stresses in various models. The increased expression of Hsps during heat shock pre-conditioning is regulated by heat shock transcription factors (HSFs). HSFs orchestrate the initiation of gene expression by binding to sequence motifs, known as cis-acting regulatory elements (CAREs). Since gene expression is regulated at a transcriptional level, it is expected that functionally related genes (e.g. heat shock response genes) might also be regulated by the same transcription factors (TFs). In this study an in silico approach was performed to identify the promoter sequences of 50 known heat shock responsive genes using Genomatix Software. This software was also used to identify transcription factor binding sites that are statistically over represented in the promoter sequences of these genes. The use of the Electrophoretic Mobility Shift Assay was included to confirm that protein cell lysates of stressed cells contain proteins (TFs) that bind to this sequence (SP1F_KLFS_01). Luciferase promoter reporter assay were also used to iii investigate the transcriptional activity of mutant promoter constructs in which the SP1F_KLFS_01 was mutated. SP1F_KLFS_01 is a ±25 base pair sequence that was identified in the promoter sequences of 19 heat shock responsive genes, including the well-known Hsp70 and Hsp90. This sequence is a potential binding site for two TFs, Specificity Protein-1 and Krueppel like TFs. Consequently, the aim of this study is to identify CAREs that are statistically over-represented in the promoter regions of heat shock response genes. In conclusion, in vitro experiments of this study did not support the findings of the in silico experiments, therefore additional methods should be implemented to expand the investigation for the involvement of cis-acting regulatory elements in the regulation of heat shock proteins in cardiomyocytes, prior to heat shock.

La grande complexité des échantillons biologiques peut compliquer l'identification des protéines et compromettre la profondeur et la couverture des analyses protéomiques utilisant la spectrométrie de masse. Des techniques de séparation permettant d’améliorer l’efficacité et la sélectivité des analyses LC-MS/MS peuvent être employées pour surmonter ces limitations. La spectrométrie de mobilité ionique différentielle, utilisant un champ électrique élevé en forme d'onde asymétrique (FAIMS), a montré des avantages significatifs dans l’amélioration de la transmission d'ions peptidiques à charges multiples, et ce, en réduisant les ions interférents. Dans ce contexte, l'objectif de cette thèse était d'explorer les capacités analytiques de FAIMS afin d'élargir à la fois la gamme dynamique de détection des protéines/peptides et la précision des mesures en protéomique quantitative par spectrométrie de masse. Pour cela, nous avons systématiquement intégré FAIMS dans des approches classiques en protéomique afin de déterminer les changements dynamiques du protéome humain en réponse à l’hyperthermie. Nous avons d’abord étudié les avantages de FAIMS par rapport à la quantification par marquage isobare (tandem mass tag, TMT). Cette approche permet le marquage d'ions peptidiques avec différents groupements chimiques dont les masses nominales sont identiques mais différant par leur distribution respective d'isotopes stables. Les ions peptidiques marqués par TMT produisent des ions rapporteurs de masses distinctes une fois fragmentés en MS/MS. Malheureusement, la co-sélection d'ions précurseurs conduit souvent à des spectres MS/MS chimériques et une approche plus lente basée sur le MS3 est nécessaire pour une quantification précise. Comme FAIMS améliore l’efficacité de séparation en transmettant sélectivement des ions en fonction de leur voltage de compensation (CV), nous avons obtenu moins de co-sélection de peptides. FAIMS a amélioré la quantification des peptides TMT au niveau MS2 et a permis d’obtenir 68% plus de peptides quantifiés par rapport aux analyses LC-MS/MS classiques, fournissant ainsi un aperçu plus vaste des changements dynamiques du protéome humain en réponse au stress thermique. De plus, nous avons étudié le marquage métabolique par incorporation d’acides aminés marqués par des isotopes stables en culture cellulaire (SILAC). Si des interférences co-éluent avec les isotopes SILAC, la quantification devient imprécise et les contreparties de SILAC peuvent être assignées de manière erronée aux ions interférants du chromatogramme, faussant ainsi le rapport SILAC. Le fractionnement post-ionisation FAIMS pourrait filtrer les ions appartenant au bruit de fond qui pourraient autrement être attribués à une paire ou à un triplet SILAC pour la quantification. Dans ce projet, FAIMS a été particulièrement bénéfique pour les espèces peu abondantes et s’est montré plus performant que le fractionnement par échange de cations (SCX). En outre, FAIMS a permis la séparation des phosphoisomères fréquemment observés dans les extraits complexes de phosphoprotéomes. Le troisième objectif de ce travail de recherche était d'explorer la séparation de l'état de charge et la transmission améliorée de peptides fortement chargés avec FAIMS et son application à l'analyse de peptides SUMOylés. FAIMS pourrait ainsi améliorer la transmission des peptides SUMOylés triplement chargés par rapport aux peptides tryptiques usuels, lesquels sont principalement doublement chargés. Ceci permettait l'enrichissement en phase gazeuse des ions peptides SUMOylés. FAIMS est une approche alternative plus simple pour fractionner les peptides SUMOylés, ce qui réduit les pertes d’échantillon et permet de simplifier le traitement des échantillons, tout en augmentant l’efficacité de séparation de manière plus automatisée et en ajoutant un ordre de grandeur de sensibilité. Le dernier objectif de cette thèse était d’améliorer l’instrumentation de FAIMS en le jumelant aux instruments à la fine pointe de la technologie. Avec un nouveau dispositif FAIMS, développé par nos collaborateurs chez Thermo Fisher Scientific, nous avons montré une amélioration dans la robustesse et la transmission des ions pour la nouvelle interface. Dans des expériences simples en protéomique shotgun, FAIMS a étendu la gamme dynamique d'un ordre de grandeur pour une couverture protéomique plus profonde par rapport aux analyses LC-MS/MS classiques. En outre, le fractionnement en phase gazeuse de FAIMS a généré moins d’analyses chimériques en MS2, ce qui a permis d’obtenir plus d’identifications et une meilleure quantification. Pour ce faire, nous avons directement comparé le LC-FAIMS-MS/MS au LC-MS/MS/MS en utilisant la sélection de précurseur synchrone (SPS) avec et sans fractionnement en phase inverse basique. Des mesures quantitatives comparables ont été obtenues pour toutes les méthodes, à l'exception du fait que FAIMS a parmi d’obtenir un nombre 2,5 fois plus grand de peptides quantifiables par rapport aux expériences sans FAIMS. Globalement, cette thèse met en évidence certains des avantages que FAIMS peut offrir aux expériences en protéomique en améliorant à la fois l'identification et la quantification des peptides.
The high complexity of biological samples can confound protein identification and compromise the depth and coverage of mass spectrometry-based proteomic analyses. Separation techniques that provide improved peak capacity and selectivity of LC-MS/MS analyses are often sought to overcome these limitations. High-field asymmetric waveform ion mobility spectrometry (FAIMS), a differential ion mobility device, has shown significant advantages by enhancing the transmission of multiple-charged peptide ions by reducing singly-charged interferences. In this context, the goal of this thesis was to explore the analytical capabilities of FAIMS to extend both the dynamic range of proteins/peptides detection and the precision of quantitative proteomic measurements by mass spectrometry. For this, we systematically integrated FAIMS in standard workflows to monitor the dynamic changes of the human proteome in response to hyperthermia. We first studied the merits of FAIMS to aid isobaric labeling quantification with tandem mass tags (TMT). This approach allows the labeling of peptide ions with different chemical groups of identical nominal masses but differing in their respective distribution of stable isotopes. TMT-labeled peptide ions produce reporter ions of distinct masses once fragmented by MS/MS. Unfortunately, the co-selection of precursor ions often leads to chimeric MS/MS spectra, and a slower MS3 centric approach is needed for precise quantification. Since FAIMS improves peak capacity by selectively transmitting ions based on their compensation voltage (CV), we obtained less peptide co-selection. FAIMS improved TMT quantification at the MS2 level and achieved 68 % more quantified peptides compared to regular LC-MS/MS, providing a deeper insight into the dynamic changes of the human proteome in response to heat stress. Further, we investigated stable isotope labeling by amino acids in cell culture (SILAC) quantification. If interferences co-elute simultaneously with SILAC isotopomers, quantification becomes inaccurate and SILAC counterparts can be missassigned to interfering ions in the highly populated chromatogram, thus skewing the SILAC ratio. FAIMS post-ionization fractionation could filter out background ions that can otherwise be attributed to a SILAC pair/triplet for quantification. In this work, FAIMS was especially beneficial for low abundant species and outperformed the standard strong cation exchange (SCX) fractionation workflow. In addition, FAIMS allowed the separation of phosphoisomers that are frequently observed in complex phosphoproteome extracts. The third aim of this work explored the charge state separation and enhanced transmission of highly charged peptides with FAIMS and its application for SUMOylated peptide analysis. FAIMS could enhance the transmission of triply charged SUMOylated peptides over typical tryptic peptide that are predominantly doubly charged, by applying more negative CVs with FAIMS. This allowed for gas-phase enrichment of SUMOylated peptide ions. FAIMS is an alternate and more straightforward approach to fractionate SUMOylated peptides that reduced sample loss, avoided sample processing, while increasing peak capacity in a more automated manner and added one order of magnitude in sensitivity. The last aim of this thesis was to improve the FAIMS instrumentation by interfacing it to the latest state-of-the-art instruments. With a new FAIMS device developed by our collaborators at Thermo Fisher Scientific, we demonstrate the robustness and the improved ion transmission for the new interface. In simple shotgun proteomics, FAIMS extended the dynamic range by one order of magnitude for deeper proteome coverage compared to regular LC-MS/MS. Moreover, fewer MS2 chimeric scans were generated with FAIMS gas-phase fractionation, which garnered more identifications and better quantification. For this, we directly compared LC-FAIMS-MS/MS to LC-MS/MS/MS using synchronous precursor selection (SPS) with and without basic reverse phase fractionation. Comparable quantitative measurements were obtained for all methods, except that FAIMS provided a 2.5-fold increase in the number of quantifiable peptides compared with non-FAIMS experiments. Overall, this thesis highlights some of the advantages that FAIMS can provide for proteomics experiments by improving both peptide identification and quantification.

Text in Afrikaans
In hierdie studie is gepoog om insig in die beroepsmobiliteit van maatskaplike werkers te verkry. Om dit te kon bereik, is eerstens 'n uitgebreide literatuurstudie onderneem en is die aard van beroepsmobiliteit, faktore wat 'n invloed op die beroepsmobiliteit van maatskaplike werkers kan he en die gevolge daarvan op maatskaplikewerkdienslewering, die maatskaplike werker en die maatskaplikewerkorganisasie verken en beskryf. Tweedens is 'n empiriese studie onderneem en is inligting oor die navorsingsonderwerp deur middel van posvraelyste as data-insamelingsinstrument by maatskaplike werkers en maatskaplikewerkorganisasies verkry. Die empiriese studie het aangedui dat maatskaplike werkers meestal aan onrealisties hoe roleise in die werksituasie en hul persoonlike lewens blootgestel is en dat die omgewings waarin hul figureer, soos die werksomgewing en persoonlike omgewings, dikwels as onvriendelik beleef word en dat dit 'n stabiele arbeidsmag verhinder. Faktore wat met die werksomstandighede van maatskaplike werkers verband hou, dra by tot die beroepsmobiliteit van maatskaplike werkers, sowel as faktore wat met persoonlike omstandighede verband hou, terwyl oorplasings en bevorderings ook 'n rol in die werksomset van maatskaplike werkers speel. Interaksie en wedersydse beinvloeding tussen die verskillende faktore vind voortdurend plaas. Daar is tot die gevolgtrekking gekom dat die maatskaplike werker, deur beroepsmobiel te wees, poog om 'n passing tussen roldruk en omgewingsdruk te bewerkstellig. Met betrekking tot die gevolge van die werksomset van maatskaplike werkers, is bevind dat maatskaplikewerkdienslewering, die maatskaplike werker en die maatskaplikewerkorganisasie positief en negatief beinvloed word wanneer maatskaplike werkers van werk verwissel of ophou om beroepsarbeid te verrig. Tendense kon deur die ondersoek vasgestel word en dit kan hydra tot die ontwikkeling van 'n teorie oor die beroepsmobiliteit van maatskaplike werkers. Aanbevelings is gemaak ten einde die beroepsmobiliteit van maatskaplike werkers te verminder en/of te voorkom
This study attempts to gain insight into the occupational mobility of social workers. In order to achieve this, an· extensive literature study was undertaken and the nature of occupational mobility, factors that could affect the occupational mobility of social workers and the consequences thereof on the delivery of social work, the social worker and the social work organisation were explored and described. Secondly, an empirical study was undertaken and information on the research topic was obtained from social workers and social work organisations through postal questionnaires as data gathering instruments. The empirical study indicated that social workers are mostly exposed to unrealistically high role demands in the work situation and in their personal lives and that the environments in which they figure, such as the work and personal environments, are often experienced as unfriendly and that these hinder a stable labour force. Factors related to the working conditions as well as to the personal situations of social workers contribute to the occupational mobility of social workers while transfers and promotions also play a part in the work turnover of social workers. Interaction and mutual influencing between the various factors take place constantly. It is concluded that occupational mobility is the result of the social workers' efforts to bring about a harmony between role and environmental pressures. With regard to the work turnover of social workers, it was found that the delivery of social work service, the social worker and the social work organisation are affected positively and negatively when social workers change jobs or cease to perform professional work. The study was able to pinpoint trends and this can contribute to the development of a theory on the occupational mobility of social workers. Recommendations were made to reduce and/or prevent the occupational mobility of social workers
Social Work
D. Phil. (Maatskaplike Werk)

For the next five years, the automotive industry is expected to change more than in the last twenty years. Scholars have for a long time developed a wide array of theories and concepts on how firms cope with changing environments and gain a competitive advantage. Contributing to the existing theories, this thesis illustrates how the leading automotive group in the Nordic countries, Møller Mobility Group, gained a competitive advantage with the launch and development of several initiatives from 2014 to 2018. This case study displays how the unprecedented changes in the automotive industry affected Møller Mobility Group and led to several alterations within the company. Combining the case with current literature, it demonstrates how the attention-based view of firm behavior inadequately explains company performance. Additionally, this case study elucidates how a consolidated multidimensional construct of dynamic capabilities better explains how firms cope with a changing environment. Finally, this thesis illustrates how firms can internally analyze their resources and capabilities when facing highly dynamic environments.
Espera-se que nos próximos cinco anos a indústria automóvel venha a sofrer alterações superiores às experienciadas nos últimos vinte anos. O universo académico tem desenvolvido ao longo dos anos um vasto número de teorias e conceitos que visam explicar como é que as empresas podem sobreviver em ambientes dinâmicos, de rápida mudança e como é que as mesmas podem adquirir vantagens competitivas. Contribuindo para as teorias recentes, esta dissertação visa ilustrar como um dos maiores grupos automóveis nos países nórdicos conseguiu manter a sua vantagem competitiva através do lançamento e desenvolvimento de múltiplas iniciativas entre 2014 e 2018. O caso de estudo do Grupo Moller Mobility demonstra como alterações sem precedente na indústria automóvel afetaram o grupo norueguês, levando a alterações profundas na empresa. Combinando este caso com a presente literatura, esta dissertação espelha como a "Attention-Based View" não é a que melhor explica a performance da empresa, sendo que o método de "Consolidated Multidimensional Construct of Dynamic Capabilities" fornece uma melhor explicação sobre a forma como as empresas podem sobreviver em contextos em permanente mudança. Por final, esta dissertação ilustra como é que as empresas podem analisar internamente os seus recursos e capacidades, face a ambientes altamente dinâmicos.

In the late 1990s the Dynamic Capabilities framework by Teece et al. (1997) shifted the focus of strategic management to an individual company’s resources and its ability to adapt them to external market trends. Refined by Barreto (2010), this resource-based view serves as a theoretical framework for this thesis, which aims at examining the development of a strategic, technological partnership between two established industrial companies. Hereby, Daimler and BMW, two of Germany’s leading automotive manufacturers are used as a real-world example in the form of a case study. The case depicts the current developments in the automotive industry and the opportunities available in the field of new mobility for traditional manufacturing companies. It outlines Daimler’s and BMW’s historical milestones and their path towards their mobility joint venture YourNow, launched in early 2019. From a strategic point of view, the case highlights the companies’ motives for the joint venture. It analyzes the exogenous shocks the companies faced, urging the former rivals to cooperate. Furthermore, the case evaluates the joint venture’s capabilities to succeed in the highly competitive mobility market as of mid 2020.
No final dos anos 1990, a framework das Dynamic Capabilities de Teece et al. (1997) mudou o foco da gestão estratégica para os recursos internos de uma empresa e para a sua capacidade de adaptá-los às tendências do mercado externo. Refinada por Barreto (2010), essa visão baseada em recursos serve como base teórica para esta tese, que visa examinar o desenvolvimento de uma parceria tecnológica estratégica entre duas empresas industriais já estabelecidas no mercado. O caso de estudo da Daimler e da BMW, dois dos principais fabricantes de automóveis da Alemanha, é utilizado como exemplo do mundo real das empresas. O caso descreve os desenvolvimentos atuais na indústria automóvel e as oportunidades disponíveis no campo da nova mobilidade para os fabricantes tradicionais. São relatados os marcos históricos da Daimler e da BMW e o seu caminho para a joint venture de mobilidade “YourNow”, lançada no início de 2019. Do ponto de vista estratégico, o caso destaca os motivos das empresas para a joint venture. Analisa ainda os choques exógenos enfrentados pelas empresas, que motivaram os antigos rivais a cooperar. Além disso, o caso avalia as capacidades da joint venture ser bem sucedida no mercado altamente competitivo da mobilidade, em meados de 2020.

A partir de un modelo de ajuste dinámico en el mercado laboral se estiman costos de movilidad intersectorial del empleo para Argentina durante el período 1996-2009, siguiendo la metodología desarrollada en Artuç, Chaudhuri and McLaren (2010). Considerando los parámetros estimados se computan simulaciones para evaluar el impacto potencial de shocks comerciales y tecnológicos sobre la asignación sectorial del empleo, los salarios sectoriales y el bienestar de los trabajadores. Nuestros resultados indican la presencia de altos costos de ajuste en el empleo, lo que implica un ajuste lento en el mercado de trabajo como respuesta a shocks tecnológicos y comerciales. Encontramos que los costos son mayores para los trabajadores no calificados respecto de los trabajadores semi calificados, no difieren entre el sector del país que comprende al Gran Buenos Aires y la Región pampeana respecto al resto del país y presentan una estructura sectorial similar a la hallada por Artuç et al. (2010) para Estados Unidos.
This paper estimates workers’ intersectoral switching costs based on a dynamic model of labor adjustment using data for Argentina (1996-2009). The estimated parameters are incorporated into a neoclassical model of trade to simulate the dynamic equilibrium impact (on welfare, wages and labor allocation) of trade shocks and technological changes. The approach used in this paper follows the method developed in Artuç, Chaudhuri and McLaren (2010). Our estimates show that Argentinian workers face high average intersectoral adjustment costs. This result suggests that the adjustment of the labor market in response to shocks is slow.