Dissertationen zum Thema „Rhizobiota“
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Fracchia, Félix. „Les phytohormones, des régulateurs clefs du microbiote du peuplier ?“ Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0217.
Der volle Inhalt der QuelleForest ecosystems are dynamic environments on both the macroscopic and microscopic scales. Trees are home to a vast array of microorganisms, called microbiota, mainly composed of bacteria and fungi. These microbial communities colonize the different tissues of trees and participate in various interactions, both detrimental (e.g. pathogens) and beneficial. Indeed, some microorganisms (e.g. Plant Growth Promoting Bacteria: PGPR; mycorrhizal fungi), improve the growth and development of their host via the transfer of nutrients, mainly nitrogen (N) and phosphorus (P) in exchange of photoassimilated sugars. On the other hand, it confers resistance to the tree in the face of biotic stresses (e.g. attack by pathogens, herbivory) and abiotic stresses (e.g. drought, soil toxicity). The assembly of this microbiota is a dynamic process in time and space. Each organ of the host constitutes a particular micro-habitat where specific microbial communities are established, both on the surface (epiphytic) and within the different compartments (endophytic). On the other hand, the establishment of the microbiota leads to a succession of microorganisms that replace the communities already present over time. There are different parameters, biotic (e.g. rhizodeposition, immunity and host genotype) and abiotic (e.g. soil type, climate, seasons), that regulate the assembly of microbial communities. In this context, the objective of this thesis is to characterize the influence of phytohormones in the assembly of poplar microbiota. We first determined the dynamics of microbial colonization of the root system of poplar seedlings from 2 to 50 days. Using two complementary methods, sequencing of bacterial (16S) and fungal (ITS, 18S) taxonomic markers, and observation of the root systems with a confocal laser scanning microscope (CLSM), we demonstrated the existence of successive waves of colonization leading to the progressive replacement of microorganisms. Using the same approaches, we characterized the colonization dynamics of the leaf microbiota. Like root systems, the assembly of microbial communities was dynamic over time. Root and aerial microorganisms were very close at early colonization times and differentiated over time. This observation suggests the transfer of microorganisms from roots to leaves leading to the selection of specific microbial communities according to host compartments. To analyze the role of phytohormones on the assembly of microbial communities, we generated transgenic lines of poplars altered in the biosynthesis and perception of gibberellic acid (GA), jasmonic acid (JA), salicylic acid (SA), ethylene (ET) and terpenes. First, we used poplar transgenic lines altered in the regulation of ET. We demonstrated that ET does not alter the composition of root exudates, in contrast to aerial and root metabolomes that were modulated according to the concentration of ET produced. On the other hand, we observed a direct and global influence of ET on the structure of the microbiota after sequencing of bacterial (ITS) and fungal (16S) taxonomic markers, and observation of root systems at CLSM. Finally, in order to exclude any cofactor that could explain microbiota variations in transgenic lines, we characterized the influence of agro-transformation without transgene expression on microbial community composition. We demonstrated that this transformation event altered the assembly of the microbiota in comparison with wild type poplars
Bourdes, Pierre-Alexandre. „Aminotransferases in rhizobia-legume symbiosis“. Thesis, University of Reading, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506071.
Der volle Inhalt der QuelleSinyanya, Kolisa Yola. „Phenotypic characterization of rhizobia isolates and distribution of Burkholderia rhizobia in the Core Cape Subregion“. Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/20335.
Der volle Inhalt der QuelleAlexandre, Ana Isabel Pereira. „Temperature stress tolerance in chickpea rhizobia“. Doctoral thesis, Universidade de Évora, 2010. http://hdl.handle.net/10174/11582.
Der volle Inhalt der QuelleBeauregard, Marie-Soleil. „Characterization of rhizobia nodulating Trifolium ambigum M.B“. Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81246.
Der volle Inhalt der QuelleCasteriano, Andrea Veronica. „Physiological mechanisms of desiccation tolerance in Rhizobia“. Thesis, The University of Sydney, 2014. http://hdl.handle.net/2123/10423.
Der volle Inhalt der QuelleHarrison, Robert. „Domesticating Lebeckia ambigua: Solving the rhizobia issues“. Thesis, Harrison, Robert (2017) Domesticating Lebeckia ambigua: Solving the rhizobia issues. Honours thesis, Murdoch University, 2017. https://researchrepository.murdoch.edu.au/id/eprint/39770/.
Der volle Inhalt der QuelleCepeda, Hernandez Martha Lucia. „Phenotypic characterization of rhizobia that nodulate ball clover“. Texas A&M University, 2005. http://hdl.handle.net/1969.1/2636.
Der volle Inhalt der QuelleSolaiman, Abu Rayhan Mohammad. „Influence of soil acidity factors on Lotus rhizobia“. Thesis, Queen's University Belfast, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356972.
Der volle Inhalt der QuelleOctive, Jerome C. „Mutagenic effects of aluminium on rhizobia and bradrhizobia“. Thesis, University of Reading, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278065.
Der volle Inhalt der QuellePfau, Thomas. „Modelling metabolic interactions in the legume-rhizobia symbiosis“. Thesis, University of Aberdeen, 2013. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=202144.
Der volle Inhalt der QuelleQu, Yihan. „Molecular Analysis of Effectiveness in Soybean-Rhizobia Symbioses“. Thesis, The University of Sydney, 2018. http://hdl.handle.net/2123/18831.
Der volle Inhalt der QuelleAmiri, Alireza Agha. „Regulation of nitrogen fixation in rhizobia-legume symbioses“. Thesis, Amiri, Alireza Agha (2021) Regulation of nitrogen fixation in rhizobia-legume symbioses. Honours thesis, Murdoch University, 2021. https://researchrepository.murdoch.edu.au/id/eprint/63559/.
Der volle Inhalt der QuelleWaldon, Hollis B. „Sonoran Desert Rhizobia Found to Nodulate Acacia constricta“. University of Arizona (Tucson, AZ), 1987. http://hdl.handle.net/10150/554231.
Der volle Inhalt der QuelleMoulin, Lionel. „Etude moléculaire de la diversité symbiotique des rhizobia : de l'analyse du gène nodA à l'identification de rhizobia au sein des bé̀ta-protéobactéries“. Lyon 1, 2002. http://www.theses.fr/2002LYO10179.
Der volle Inhalt der QuellePeele, Price Jason. „Control of RNA Structure by CspA Proteins in Rhizobia“. Thesis, Washington State University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10605605.
Der volle Inhalt der QuelleRhizobia are soil bacteria that can associate with some legumes and participate in symbiotic nitrogen fixation. Bacterial CspA family members are small, single stranded nucleic acid binding proteins conserved throughout all domains of life. Here, the role of CspA family proteins in the symbiotic development of Sinorhizobium meliloti with Medicago sativa (alfalfa) is investigated. Expression and genetic deletion strain analysis revealed that CspA family proteins are differentially expressed in symbiosis and contribute to symbiotic effectiveness. RNAseq analysis of native co-immunoprecipitated RNAs identified a novel interaction between several CspA family proteins and the αR14 family of small non-coding RNA (sRNAs). Whole transcriptome analysis defined transcriptional defects associated with loss of CspA function. The development of a new in vitro RNA binding assay using broccoli, a Green Fluorescent Protein (GFP) RNA mimic, is described as well as its use in defining binding specificity of CspA family proteins with synthetic and native ?R14 family sRNA structures. This work concludes that CspA family proteins interact with and influence the stability of specific RNA structures and these interactions control RNA regulated processes important for symbiotic development.
Morieri, Giulia. „Two types of calcium signalling in legume-rhizobia symbiosis“. Thesis, University of East Anglia, 2010. https://ueaeprints.uea.ac.uk/31683/.
Der volle Inhalt der QuelleLaranjo, Marta. „Genetic diversity and symbiotic effectiveness of chickpea rhizobia strains“. Doctoral thesis, Universidade de Évora, 2007. http://hdl.handle.net/10174/11260.
Der volle Inhalt der QuelleSingh, Guriqbal. „Effects of herbicides on nodulation, biological nitrogen fixation and growth of peas (Pisum sativum L.)“. Thesis, Bangor University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262763.
Der volle Inhalt der QuelleRao, J. Raghavendra. „Selection of fast- or slow-growing rhizobia by Lotus species“. Thesis, Queen's University Belfast, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317117.
Der volle Inhalt der QuelleWebster, Gordon. „The interaction between rhizobia and the non-legume Parasponia andersonii“. Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283646.
Der volle Inhalt der QuelleMutch, Lesley Anne. „Molecular ecology of rhizobia isolated from native and cultivated Vicieae“. Thesis, University of York, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313847.
Der volle Inhalt der QuelleSilva, ValÃria Maria AraÃjo. „Facilitation can increase actinobacteria adaptation capacity and rhizobia "in vitro"“. Universidade Federal do CearÃ, 2016. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=17158.
Der volle Inhalt der QuelleThe natural environment is marked by an intricate network of biotic interactions that shape the structure of ecological communities. The presence of positive ecological interactions between microbial populations in soils semiarid regions, has great importance in structuring the local soil microbiota. In this work, actinomycetes strains of rhizobia and coming from rhizosphere of Park National Ubajara-CE, were evaluated for the ability to grow through cooperative metabolic mechanisms. Of the 27 evaluated actinomycetes, 22 showed compatibility with rhizobia. The strains UB-05, UB-07, UB-08, UB-11 and UB-21 stood out in facilitating tests for amylase and cellulase. The metabolic activity of actinomycetes helped the development of rhizobia strains
O ambiente natural à marcado por uma intrincada rede de interaÃÃes biÃticas que moldam a estrutura das comunidades ecolÃgicas. A presenÃa de interaÃÃes ecolÃgicas positivas entre populaÃÃes microbianas em solos de regiÃes semiÃridas, possui grande relevÃncia na estruturaÃÃo da microbiota do solo local. Neste trabalho, cepas de actinobactÃrias e rizÃbios oriundas de solo rizosfÃrico do Parque Nacional de Ubajara-CE, foram avaliadas quanto à capacidade de crescerem atravÃs de mecanismos metabÃlicos cooperativos. Das 27 actinobactÃrias avaliadas, 22 apresentaram compatibilidade com rizÃbios. As cepas UB-05, UB-07, UB-08, UB-11 e UB-21, destacaram-se nos ensaios de facilitaÃÃo para amilase e celulase. A atividade metabÃlica de actinobactÃrias auxiliou o desenvolvimento das cepas de rizÃbios.
Silva, José Rodrigo da. „Improvement of chickpea rhizobia by genetic transformation with symbiosis genes“. Doctoral thesis, Universidade de Évora, 2018. http://hdl.handle.net/10174/23169.
Der volle Inhalt der QuelleBenezech, Claire. „Développement et étude de systèmes d'interactions tripartites, légumineuses-rhizobia-pathogènes“. Thesis, Toulouse 3, 2019. http://www.theses.fr/2019TOU30289.
Der volle Inhalt der QuelleNitrogen is essential element for the development of all living beings. Although it is found in large quantity in the air, in the form of dinitrogen, it is not directly assimilable by most organisms. For example, plants are not able to assimilate this form. However, in a nitrogen deficient environment, legumes are able to interact with soil borne bacteria, rhizobia, which fix nitrogen thanks to an enzymatic complex, the nitrogenase. Indeed, bacteria reduce dinitrogen in ammonium; plants can assimilate this form. Plants host these bacteria in particular organs at the root level, the nodules, where they provide nutrients to bacteria. Plant tolerates in its own cells a tremendous quantity of foreign organisms, estimated to one billion of rhizobia per nodule. The massive bacterial colonization of nodules is allowed thanks to the repression of plant immunity. Roots are in contact with the abundant soil microbiota, which raises the question of the potential vulnerability of the symbiotic organs and nodulated plants. The phD project aimed to evaluate the nodules vulnerability. To achieve this, we set up two tripartite systems involving the model legume, Medicago truncatula, its symbiont, Sinorhizobium medicae and separately two phytopathogenic microorganisms, a bacterium, Ralstonia solancearum and the fungus, Sclerotinia sclerotiorum. We also characterized nodules responses to both pathogens using roots as reference. Finally, we estimated the influence of nodulation and nitrogen fixation on the plant vulnerability to pathogens. Work performed during these three years indicates that nodules are infection sites for pathogens. Those nodules are able to perceive the pathogen however, their response is different and less intense than that of roots. Results obtained with one of our tripartite system suggest that nodulation and nitrogen fixation give a greater sensitivity to pathogens
Chafchaouni-Bussy, Moussaoui Imane. „Etude de l’implication des lipopolysaccharides dans la Symbiose Bactérie-Plante productrice d’azote“. Thesis, Paris 11, 2011. http://www.theses.fr/2011PA11T045.
Der volle Inhalt der QuelleWe were interested in the understanding of the mechanisms governing Rhizobium-Acacia symbiosis in salt stress conditions. Lipopolysaccharides play an important role in the stages of this symbiosis. The aim of this work was to highlight the changes occurring in the bacterial membrane in response to salt stress by studying the structure of the lipopolysaccharides isolated from Moroccan desert strains tolerating 7% NaCl. Thus, a new method of hydrolysis of the lipopolysaccharide - sensitive, non-destructive and compatible with mass spectrometry- was developed. We studied the LPSs strains grown with or without salt stress and we showed that in salt stress conditions, the outer membrane becomes more hydrophobic by increasing acylation of the lipid region and reducing the number of long sugar chains in LPSs. Tests for evaluating the efficiency and infectivity of the studied rhizobia were carried out to determine the impact of these LPS modifications on symbiosis under salt stress
Abdul, Jalil A. I. „The growth and colonisation of rhizobia on mycorrhizal Trifolium repens (L.)“. Thesis, University of Reading, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370869.
Der volle Inhalt der QuelleAlexandre, Ana, und Solange Oliveira. „Heat shock response in bacteria with large genomes: lessons from rhizobia“. Bachelor's thesis, Wiley-Blackwell Publishers, 2016. http://hdl.handle.net/10174/19210.
Der volle Inhalt der QuelleTroyee, Anupoma Niloya. „Impact of soil treatments in the conservation of native rhizobia populations“. Master's thesis, Universidade de Évora, 2017. http://hdl.handle.net/10174/22076.
Der volle Inhalt der QuelleSalo, Lucinda Faith. „COMPETITION BETWEEN STRAINS OF RHIZOBIA FOR NODULATION OF LEUCAENA LEUCOCEPHALA (LEGUME)“. Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275326.
Der volle Inhalt der QuelleSublett, Jacob D. „Effects of seed coat variation and population on plant-microbial interactions“. Bowling Green State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1467569697.
Der volle Inhalt der QuelleHsu, Chiun-Kang. „EVALUATION OF SEED TREATMENTS ON THE NODULE COMPETENCY OF SOYBEAN INOCULANTS“. OpenSIUC, 2014. https://opensiuc.lib.siu.edu/dissertations/823.
Der volle Inhalt der QuelleBala, Abdullahi. „Biodiversity of rhizobia which nodulate fast-growing tree legumes in tropical soils“. Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391891.
Der volle Inhalt der QuelleBontemps, Cyril. „Exploration et analyse de la diversité des rhizobia : nouveaux outils et concepts“. Lyon 1, 2005. http://www.theses.fr/2005LYO10224.
Der volle Inhalt der QuelleSangay-Tucto, Sheena. „Étude de l’impact des symbioses mycorhizienne et rhizobienne dans la domestication du Tara, Caesalpinia spinosa L“. Thesis, Montpellier, 2018. http://www.theses.fr/2018MONTG080/document.
Der volle Inhalt der QuelleThe Tara (Caesalpinia spinosa) is a forest species of great importance due to its high demand in the international market for the tannins present in its pods and its seeds’ gum. Despite its great importance for Peru, most of the production comes from unmanaged natural forests. These forests present soil problems (e.g., erosion, low fertility, pathogens, lack of irrigation), which cause low yields. Therefore, in the present work we seek to study the soil components associated with Tara plantation , such as mycorrhizae and bacteria that have proved to be an alternative for reducing the use of chemical fertilizers in similar context (Aboubacar et al., Flores Chavez 20015, E and Saif 1987, Dia et al. 2010; Bilgo et al., 2013) . We used molecular analysis of the arbuscular diversity by the Miseq Illumina technique that allowed to verify the arbuscular diversity with a preponderance of Glomeraceae among which the Rhizophagus spp were found to be present in 70% of the sequences. In addition, the dependence of the Tara on obligatory mycorrhization was demonstrated, after testing the controlled mycorrhization of the Tara by the Rhizophagus irregularis. We found that the growth of this crop was significantly improved, as well as the absorption of nutrients such as nitrogen (N) and phosphorus (P).To check the nodulation of the Tara, different culture media were used (JenSen, sand mixture with Tara plantation soil, attapulgite mixture with Tara plantation soils) in greenhouse and in vitro condition. We did not manage to find rhizobial nodules in the roots which let us think that Tara is a non-nodular legume. Therefore, we used Pisum sativum as a trap plant to study the diversity of rhizobia present in the soil of the Tara plantation since this legume is often associated with Tara crop. The rhizobia found in the trap plant were very specific and different from the rhizobia present in soils outside the Tara plantation. Likewise, these rhizobia found to be phylogenetically close to R. etli, R. phaseoli, R. pisi and R. leguminosarum. Finally, we inoculated the trapped bacterias (in vitro) in Pisum sativum with the bacterias previously trapped and isolated from the pea (which grew in the green house); where it was observed that the rhizospheric bacteria of the zones IL (soil collected between two lines) and L (soil collected from the same line) from the plantation of Tara stimulated the growth of this crop with respect to the bacteria present in soils collected outside of the plantation (OP zone)
Miller, Simon Hugh, und n/a. „Genetic basis for the host-specific nitrogen fixation phenotype of Caucasian clover rhizobia“. University of Otago. Department of Microbiology, 2006. http://adt.otago.ac.nz./public/adt-NZDU20070306.155157.
Der volle Inhalt der QuelleO'Callaghan, Kenneth John. „Interactions of rhizobia with Sesbania rostrata, wheat and oilseed rape : a comparative study“. Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285558.
Der volle Inhalt der QuelleSpriggs, AC, und FD Dakora. „Symbiotic performance of selected Cyclopia Vent. (honeybush) rhizobia under nursery and field conditions“. Symbiosis, 2009. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1001479.
Der volle Inhalt der QuelleShamsuddin, Zulkifli Haji. „Growth, infectivity and nodulating abilities of some winged bean rhizobia in acid conditions“. Thesis, Shamsuddin, Zulkifli Haji (1987) Growth, infectivity and nodulating abilities of some winged bean rhizobia in acid conditions. PhD thesis, Murdoch University, 1987. https://researchrepository.murdoch.edu.au/id/eprint/51958/.
Der volle Inhalt der QuelleGodschalx, Adrienne Louise. „Symbiosis with Nitrogen-fixing Rhizobia Influences Plant Defense Strategy and Plant-predator Interactions“. PDXScholar, 2017. https://pdxscholar.library.pdx.edu/open_access_etds/3644.
Der volle Inhalt der QuelleOtieno, Mary Atieno. „Proteomic Changes in Rhizobia after Growth in Peat Extract and Their Potential Role in Desiccation Tolerance“. Thesis, The University of Sydney, 2017. http://hdl.handle.net/2123/17791.
Der volle Inhalt der QuelleMaghraoui, Tasnime. „Diversité génotypique et fonctionnelle des rhizobia associés aux cultures mixtes Fève-Blé en conditions limitantes de phosphore au Maroc“. Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT147.
Der volle Inhalt der QuelleThe main purpose of this study is to evaluate the role of phosphate solubilizing rhizobial strains in promoting faba bean (legume) and wheat (cereal) growth in mono and mixed cultures under phosphorus deficiency conditions. 109 rhizobial strains have been isolated from nodules of V. faba plants in Marrakech region. Phylogenetic analysis based on 16S rDNA, recA and nodD genes showed that the studied strains are close to Rhizobium leguminosarum biovar viciae, R. laguerreae and Ensifer meliloti. We detected the presence of pqqC gene which is implicated on phosphate solubilization (Pyrroloquinoline-quinone synthase C) in 15 rhizobia strains. 22 % of isolated strains were found to be able to solubilize mineral phosphate. We have demonstrated that the inoculation with some of rhizobial strains with PSolubilization capacity in vitro, improved the growth of fava bean and wheat plants in greenhouse (both in mono and mixed cultures). We noticed different reactions to the inoculation's effect, depending on the symbiotic combinations faba bean-rhizobia studied. We also showed that rhizobial strains can stimulate the transfer of fixed nitrogen from the bean to the wheat, which could contribute significantly to the plant-plant facilitation process in associated cultures with phosphorus deficiency conditions. Besides this association advantages, this cultural practice is not limited to a trophic effect (improvement of the nitrogenous nutrition of plants), but could engender microbial interactions which can directly enhance the wheat growth (PGPR effect). The results of the experiments conducted in greenhouse and on field show that mixed cultures stimulate wheat growth and its mineral nutrition (N and P). These results underline the utility of developing this kind of cultural practices associating legumes and cereals, and to use the local microbial resources in the agro-ecosystems to improve the agricultural production by reducing the use of agricultural improvers among others expensive artificial fertilizers which represent a threat to the environment
Melkonian, Rémy. „Etude de la spécificité d’association béta-rhizobia-Mimosa : approches par l'écologie microbienne et la génomique fonctionnelle“. Thesis, Montpellier 2, 2012. http://www.theses.fr/2012MON20195/document.
Der volle Inhalt der QuelleBeta-rhizobia are legume symbionts mainly found associated to the Mimosa genus. Diversity studies of Mimosa pudica symbionts in native and introduced areas reveal different diversity patterns of alpha (Rhizobium spp) and beta-rhizobia (Burkholderia, Cupriavidus), with beta-rhizobia being always the main symbionts in the nodules of this legumes species. In this thesis we have studied the symbiotic specificity between beta-rhizobia and M. pudica (and the comparison with alpha-rhizobia) by a dual approach combining the study of bacterial symbiotic traits and the analysis of their transcriptomes in the first steps of symbiosis. We analysed symbiotic traits (nodulation competitiveness, symbiotic efficiency) at intra and interspecific levels on four species of beta-rhizobia and four of alpha-rhizobia. If symbiotic efficiency is similar among all strains, different levels of competitiveness were measured with a strong strain effect largely explained by the species affiliation, B. phymatum and B. tuberum being the most competitive species. Tests on different M. pudica varieties showed an impact on the competitiveness of C. taiwanensis. Symbiotic traits explained in part the symbiont patterns observed in diversity studies in French Guiana (M. pudica native area) and Taiwan (introduced). Root-exudates induced transcriptomes of three bacteria (two beta--rhizobia: B. phymatum STM815, C. taiwanensis LMG19424 and one alpha, R. mesoamericanum STM3625) with contrasted symbiotic traits were compared (by RNAseq). Each bacterium develops a specific strategy linked to its symbiotic traits and the origin of symbiosis in its bacterial group
Domergue, Odile. „Diversité fonctionnelle des rhizobia associés à la féverole en agro-écosystème Sud de France“. Thesis, Paris Sciences et Lettres (ComUE), 2017. http://www.theses.fr/2017PSLEP067/document.
Der volle Inhalt der QuelleThe ability of rhizobium-legume symbiosis to fix atmospheric nitrogen can provide important services to agro-ecosystems. However, associated with biotic constraints (i) missing and/or (ii) phosphorus (P) deficiency, can limit symbiotic nitrogen fixation (SNF). Myo-inositol hexakisphosphate (phytate), the main soil organic P (Po) component, represents a potential source of soil P available for plant P bio-availability, connected with microbial phytate-mineralizing activities. In order to look for rhizobium-Vicia faba, in P use efficiency (PUE) for SNF ability, nodular diagnosis was performed from a multi-location survey in an agro-ecosystem in South France. The efficiency in use of rhizobial symbiosis (EURS) was deduced from V. faba nodule and shoot dry weight values at flowering state, and compared in intra- and inter agricultural fields and in greenhouse hydroaeroponic conditions. Among 59 V. faba isolates, 26 were able to mineralize phytate (Phy+) in solid medium and six of them confirmed phytase activity in liquid culture. Looking for local rhizobiums with PUE for SNF ability, led us to a forward step in molecular marker selection for Phy+ rhizobium isolate screening. KeywordsRhizobium, diversity, phytase, faba-bean, Vicia faba, nodulation, South-France, agro-ecosystem
Westhoek, Annet. „Resource allocation in the legume-rhizobia symbiosis : an integration of modelling and experimental approaches“. Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:66ed2e7d-85d3-4090-a822-28609ea866c7.
Der volle Inhalt der QuellePillai, Suresh Divakaran. „Ecology and genetic stability of Tn5 mutants of bean rhizobia in Sonoran desert soils“. Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184823.
Der volle Inhalt der QuelleMiller, Lance Delano. „Characterization of the Chemotaxis System of the Endosymbiotic Bacterium Rhizobium leguminosarum“. Diss., Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/19707.
Der volle Inhalt der QuelleBashore, Sarah L. aity. „Characterization of a Spontaneous Phaseolus Vulgaris Mutant with the Ability to Selectively Restrict Nodulation“. Ohio University / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1155747948.
Der volle Inhalt der QuelleForquer, Isaac P. „Characterization of Photosynthetic Reaction Centers from Bradyrhizobium strain BTAi 1“. Wright State University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=wright1133541454.
Der volle Inhalt der QuelleMaynaud, Géraldine. „Adaptation aux métaux lourds de populations de rhizobia impliquées dans la phytostabilisation de déblais miniers : Identification des mécanismes d’adaptation au Zn et au Cd, et structuration des populations de rhizobia adaptées aux sites miniers“. Thesis, Montpellier 2, 2012. http://www.theses.fr/2012MON20074/document.
Der volle Inhalt der QuelleEfficient nitrogen-fixing symbiosis between Anthyllis vulneraria and Mesorhizobium metallidurans, identified in the highly Zn/Pb polluted mining site of Avinières (St Laurent-le-Minier, Gard county, France) has recently been described as a potential key bioremediation agent for stimulating the growth of a sustainable plant cover and thus limit heavy metal dispersion from contaminated sites. M. metallidurans strains were shown to be resistant to high Zn and Cd concentrations. The aim of our work was to identify and characterize genes involved in heavy metal adaptation in M. metallidurans by using genetic and transcriptomic approaches (RNAseq technology). Putative genes involved in heavy metal adaptation mechanisms such as exclusion, binding, reduction and efflux, like cadA1, encoding an efflux system PIB-type ATPase involved in Zn and Cd export, were identified in two Mesorhizobium strains associated with Anthyllis: M. metallidurans STM 2683T (Avinières mine) and Mesorhizobium sp. STM 4661 (Eylie mine). Functional studies allowed us to characterize the cadA1 efflux protein as involved in metal tolerance in M. metallidurans. Then, cadA1 was used as a metal-resistance marker to study the diversity and the distribution of Anthyllis symbionts from mine soils and unpolluted soils. This work was completed by Zn- and Cd-tolerance phenotype assays and phylogenetic analyses using taxonomical and symbiotic markers. Metals in mine environments seemed to influence the bacterial composition and the diversity with a high proportion of (i) metal-tolerant phenotypes consistent with the detection of the cadA1 gene and (ii) strains belonging to the M. metallidurans species or to a bacterial species close to it. The plant-hosts seemed to impact symbiotic diversity independently of the metal-tolerant property