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Zeitschriftenartikel zum Thema "Promoting rhizobacteria"

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Kashyap, Abhijeet Shankar, Nazia Manzar, Mahendra Vikram Singh Rajawat, Amit Kumar Kesharwani, Ravinder Pal Singh, S. C. Dubey, Debasis Pattanayak, Shri Dhar, S. K. Lal und Dinesh Singh. „Screening and Biocontrol Potential of Rhizobacteria Native to Gangetic Plains and Hilly Regions to Induce Systemic Resistance and Promote Plant Growth in Chilli against Bacterial Wilt Disease“. Plants 10, Nr. 10 (07.10.2021): 2125. http://dx.doi.org/10.3390/plants10102125.

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Plant growth-promoting rhizobacteria (PGPR) is a microbial population found in the rhizosphere of plants that can stimulate plant development and restrict the growth of plant diseases directly or indirectly. In this study, 90 rhizospheric soil samples from five agro climatic zones of chilli (Capsicum annuum L.) were collected and rhizobacteria were isolated, screened and characterized at morphological, biochemical and molecular levels. In total, 38% of rhizobacteria exhibited the antagonistic capacity to suppress Ralstonia solanacearum growth and showed PGPR activities such as indole acetic acid production by 67.64% from total screened rhizobacteria isolates, phosphorus solubilization by 79.41%, ammonia by 67.75%, HCN by 58.82% and siderophore by 55.88%. We performed a principal component analysis depicting correlation and significance among plant growth-promoting activities, growth parameters of chilli and rhizobacterial strains. Plant inoculation studies indicated a significant increase in growth parameters and PDS1 strain showed maximum 71.11% biocontrol efficiency against wilt disease. The best five rhizobacterial isolates demonstrating both plant growth-promotion traits and biocontrol potential were characterized and identified as PDS1—Pseudomonas fluorescens (MN368159), BDS1—Bacillus subtilis (MN395039), UK4—Bacillus cereus (MT491099), UK2—Bacillus amyloliquefaciens (MT491100) and KA9—Bacillus subtilis (MT491101). These rhizobacteria have the potential natural elicitors to be used as biopesticides and biofertilizers to improve crop health while warding off soil-borne pathogens. The chilli cv. Pusa Jwala treated with Bacillus subtilis KA9 and Pseudomonas fluorescens PDS1 showed enhancement in the defensive enzymes PO, PPO, SOD and PAL activities in chilli leaf and root tissues, which collectively contributed to induced resistance in chilli plants against Ralstonia solanacearum. The induction of these defense enzymes was found higher in leave tissues (PO—4.87-fold, PP0—9.30-fold, SOD—9.49-fold and PAL—1.04-fold, respectively) in comparison to roots tissue at 48 h after pathogen inoculation. The findings support the view that plant growth-promoting rhizobacteria boost defense-related enzymes and limit pathogen growth in chilli plants, respectively, hence managing the chilli bacterial wilt.
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Glick, Bernard R. „The enhancement of plant growth by free-living bacteria“. Canadian Journal of Microbiology 41, Nr. 2 (01.02.1995): 109–17. http://dx.doi.org/10.1139/m95-015.

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The ways in which plant growth promoting rhizobacteria facilitate the growth of plants are considered and discussed. Both indirect and direct mechanisms of plant growth promotion are dealt with. The possibility of improving plant growth promoting rhizobacteria by specific genetic manipulation is critically examined.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria.
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Liu, Ying, Jie Gao, Zhihui Bai, Shanghua Wu, Xianglong Li, Na Wang, Xiongfeng Du et al. „Unraveling Mechanisms and Impact of Microbial Recruitment on Oilseed Rape (Brassica napus L.) and the Rhizosphere Mediated by Plant Growth-Promoting Rhizobacteria“. Microorganisms 9, Nr. 1 (12.01.2021): 161. http://dx.doi.org/10.3390/microorganisms9010161.

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Plant growth-promoting rhizobacteria (PGPR) are noticeably applied to enhance plant nutrient acquisition and improve plant growth and health. However, limited information is available on the compositional dynamics of rhizobacteria communities with PGPR inoculation. In this study, we investigated the effects of three PGPR strains, Stenotrophomonas rhizophila, Rhodobacter sphaeroides, and Bacillus amyloliquefaciens on the ecophysiological properties of Oilseed rape (Brassica napus L.), rhizosphere, and bulk soil; moreover, we assessed rhizobacterial community composition using high-throughput Illumina sequencing of 16S rRNA genes. Inoculation with S. rhizophila, R. sphaeroides, and B. amyloliquefaciens, significantly increased the plant total N (TN) (p < 0.01) content. R. sphaeroides and B. amyloliquefaciens selectively enhanced the growth of Pseudomonadacea and Flavobacteriaceae, whereas S. rhizophila could recruit diazotrophic rhizobacteria, members of Cyanobacteria and Actinobacteria, whose abundance was positively correlated with inoculation, and improved the transformation of organic nitrogen into inorganic nitrogen through the promotion of ammonification. Initial colonization by PGPR in the rhizosphere affected the rhizobacterial community composition throughout the plant life cycle. Network analysis indicated that PGPR had species-dependent effects on niche competition in the rhizosphere. These results provide a better understanding of PGPR-plant-rhizobacteria interactions, which is necessary to develop the application of PGPR.
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García de Salamone, Ines E., Russell K. Hynes und Louise M. Nelson. „Cytokinin production by plant growth promoting rhizobacteria and selected mutants“. Canadian Journal of Microbiology 47, Nr. 5 (01.05.2001): 404–11. http://dx.doi.org/10.1139/w01-029.

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One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20–18, a rifampicin-resistant mutant (RIF), and two TnphoA-derived mutants (CNT1, CNT2), with reduced capacity to synthesize cytokinins, was further characterized in pure culture using immunoassay and thin layer chromatography. G20–18 produced higher amounts of three cytokinins, isopentenyl adenosine (IPA), trans-zeatin ribose (ZR), and DHZR than the three mutants during stationary phase. IPA was the major metabolite produced, but the proportion of ZR and DHZR accumulated by CNT1 and CNT2 increased with time. No differences were observed between strain G20–18 and the mutants in the amounts of indole acetic acid synthesized, nor were gibberellins detected in supernatants of any of the strains. Addition of 10–5 M adenine increased cytokinin production in 96- and 168-h cultures of strain G20–18 by approximately 67%. G20–18 and the mutants CNT1 and CNT2 may be useful for determination of the role of cytokinin production in plant growth promotion by PGPR.Key words: cytokinins, plant growth regulation, Pseudomonas fluorescens, rhizobacteria, plant growth promoting rhizobacteria (PGPR).
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Yanti, Yulmira, Trimurti Habazar, Zurai Resti und Dewi Suhalita. „PENAPISAN ISOLAT RIZOBAKTERI DARI PERAKARAN TANAMAN KEDELAI YANG SEHAT UNTUK PENGENDALIAN PENYAKIT PUSTUL BAKTERI (Xanthomonas axonopodis pv. glycines)“. Jurnal Hama dan Penyakit Tumbuhan Tropika 13, Nr. 1 (10.01.2013): 24–34. http://dx.doi.org/10.23960/j.hptt.11324-34.

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Screening of indigenous rhizobacteria from healthy soybean root to control bacterial pustule (Xanthomonas axonopodis pv. glycines) using in planta technique. Plant growth promoting rhizobacteria are a group of bacteria that actively colonize plant roots, increase plant growth and control plant pathogens. The aim of this study was to obtain rhizobacteri isolates which have the ability to control bacterial pustule and increase growth and yield of soybean. This method based on in planta selection of enhanced competitive soil root-colonizing bacteria from soil samples of healthy soybean root at endemic area of bacterial pustule in Darmasraya District and Sijunjung District, West Sumatera. We characterized only the best rhizobacteri isolates which have ability to control bacterial pustule and to increase growth and yield of soybean. This type of characterization has possibility to find new, easy and cheap biocontrol organisms. Ten Rhizobacteri isolates were introduced via seed treatment (108 cfu/ml) and soil drench to 3 week old soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated to one month old of soybean seedling. The effect of rhizobacteria on disease incidence, disease severity, plant growth and yield of soybean were evaluated. We have found that two selected rhizobacteri isolates from soybean (P12Rz2.1 and P14Rz1.1) were the best isolates in promoting growth and the of the soybean plants with the effectiveness 20.62 % and 20.47 %.
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Lugtenberg, Ben, und Faina Kamilova. „Plant-Growth-Promoting Rhizobacteria“. Annual Review of Microbiology 63, Nr. 1 (Oktober 2009): 541–56. http://dx.doi.org/10.1146/annurev.micro.62.081307.162918.

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Miransari, Mohammad. „Plant Growth Promoting Rhizobacteria“. Journal of Plant Nutrition 37, Nr. 14 (30.08.2014): 2227–35. http://dx.doi.org/10.1080/01904167.2014.920384.

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Singh, Jay Shankar. „Plant Growth Promoting Rhizobacteria“. Resonance 18, Nr. 3 (März 2013): 275–81. http://dx.doi.org/10.1007/s12045-013-0038-y.

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Sharma, Vriti, Aakriti Singh, Diksha Sharma, Aashima Sharma, Sarika Phogat, Navjyoti Chakraborty, Sayan Chatterjee und Ram Singh Purty. „Stress mitigation strategies of plant growth-promoting rhizobacteria: Plant growth-promoting rhizobacteria mechanisms“. Plant Science Today 8, sp1 (12.02.2022): 25–32. http://dx.doi.org/10.14719/pst.1543.

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One of the major challenges that the world is facing currently is the inadequate amount of food production with high nutrient content in accordance with the increase in population size. Moreover, availability of cultivable area with fertile soil is reducing day by day owing to ever increasing population. Further, water scarcity and expensive agricultural equipment have led to the use of agrochemicals and untreated water. Excessive use of chemical fertilizers to increase crop yield have resulted in deleterious effects on the environment, health and economy, which can be overcome to a great extent by employing biological fertilizers. There are various microbes that grows in the rhizospheric region of plants known as plant growth-promoting rhizobacteria (PGPR). PGPR act by direct and indirect modes to stimulate plant growth and improve stress reduction in plants. PGPRs are used for potential agriculture practices having a wide range of benefits like increase in nutrients content, healthy growth of crops, production of phytohormones, prevention from heavy metal stress conditions and increase in crop yield. This review reports recent studies in crop improvement strategies using PGPR and describes the mechanisms involved. The potential mechanisms of PGPR and its allies pave the way for sustainable development towards agriculture and commercialization of potential bacteria.
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Saeed, Qudsia, Wang Xiukang, Fasih Ullah Haider, Jiří Kučerik, Muhammad Zahid Mumtaz, Jiri Holatko, Munaza Naseem et al. „Rhizosphere Bacteria in Plant Growth Promotion, Biocontrol, and Bioremediation of Contaminated Sites: A Comprehensive Review of Effects and Mechanisms“. International Journal of Molecular Sciences 22, Nr. 19 (29.09.2021): 10529. http://dx.doi.org/10.3390/ijms221910529.

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Agriculture in the 21st century is facing multiple challenges, such as those related to soil fertility, climatic fluctuations, environmental degradation, urbanization, and the increase in food demand for the increasing world population. In the meanwhile, the scientific community is facing key challenges in increasing crop production from the existing land base. In this regard, traditional farming has witnessed enhanced per acre crop yields due to irregular and injudicious use of agrochemicals, including pesticides and synthetic fertilizers, but at a substantial environmental cost. Another major concern in modern agriculture is that crop pests are developing pesticide resistance. Therefore, the future of sustainable crop production requires the use of alternative strategies that can enhance crop yields in an environmentally sound manner. The application of rhizobacteria, specifically, plant growth-promoting rhizobacteria (PGPR), as an alternative to chemical pesticides has gained much attention from the scientific community. These rhizobacteria harbor a number of mechanisms through which they promote plant growth, control plant pests, and induce resistance to various abiotic stresses. This review presents a comprehensive overview of the mechanisms of rhizobacteria involved in plant growth promotion, biocontrol of pests, and bioremediation of contaminated soils. It also focuses on the effects of PGPR inoculation on plant growth survival under environmental stress. Furthermore, the pros and cons of rhizobacterial application along with future directions for the sustainable use of rhizobacteria in agriculture are discussed in depth.
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Dissertationen zum Thema "Promoting rhizobacteria"

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Davies, Keith Graham. „Studies on plant growth promoting rhizobacteria“. Thesis, Bangor University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266612.

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Noh, Medina José Alfredo. „Rhizobacteria promoting the growth of plants infected with viruses“. Thesis, Université Laval, 2007. http://www.theses.ulaval.ca/2007/24319/24319.pdf.

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Lewis, Ricky W. „TOXICITY OF ENGINEERED NANOMATERIALS TO PLANT GROWTH PROMOTING RHIZOBACTERIA“. UKnowledge, 2016. http://uknowledge.uky.edu/pss_etds/77.

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Engineered nanomaterials (ENMs) have become ubiquitous in consumer products and industrial applications, and consequently the environment. Much of the environmentally released ENMs are expected to enter terrestrial ecosystems via land application of nano-enriched biosolids to agricultural fields. Among the organisms most likely to encounter nano-enriched biosolids are the key soil bacteria known as plant growth promoting rhizobacteria (PGPR). I reviewed what is known concerning the toxicological effects of ENMs to PGPR and observed the need for high-throughput methods to evaluate lethal and sublethal toxic responses of aerobic microbes. I addressed this issue by developing high-throughput microplate assays which allowed me to normalize oxygen consumption responses to viable cell estimates. Oxygen consumption is a crucial step in cellular respiration which may be examined relatively easily along with viability and may provide insight into the metabolic/physiological response of bacteria to toxic substances. Because many of the most toxic nanomaterials (i.e. metal containing materials) exhibit some level of ionic dissolution, I first developed my methods by examining metal ion responses in the PGPR, Bacillus amyloliquefaciens GB03. I found this bacterium exhibits differential oxygen consumption responses to Ag+, Zn2+, and Ni2+. Exposure to Ag+ elicited pronounced increases in O2 consumption, particularly when few viable cells were observed. Also, while Ni2+ and Zn2+ are generally thought to induce similar toxic responses, I found O2 consumption per viable cell was much more variable during Ni2+ exposure and that Zn2+ induced increased O2 utilization to a lesser extent than Ag+. Additionally, I showed my method is useful for probing toxicity of traditional antibiotics by observing large increases in O2 utilization in response to streptomycin, which was used as a positive control due to its known effects on bacterial respiration. After showing the utility of my method for examining metal ion responses in a single species of PGPR, I investigated the toxicity of silver ENMs (AgENMs) and ions to three PGPR, B. amyloliquefaciens GB03, Sinorhizobium meliloti 2011, and Pseudomonas putida UW4. The ENM exposures consisted of untransformed, polyvinylpyrrolidone coated silver ENMs (PVP-AgENMs) and 100% sulfidized silver ENMs (sAgENMs), which are representative of environmentally transformed AgENMs. I observed species specific O2 consumption responses to silver ions and PVP-AgENMs. Specifically, P. putida exhibited increased O2 consumption across the observed range of viable cells, while B. amyloliquefaciens exhibited responses similar to those found in my first study. Additionally, S. meliloti exhibited more complex responses to Ag+ and PVP-AgENMs, with decreased O2 consumption when cell viability was ~50-75% of no metal controls and increased O2 consumption when cell viability was <50%. I also found the abiotically dissolved fraction of the PVP-AgENMs was likely responsible for most of the toxic response, while abiotic dissolution did not explain the toxicity of sAgENMs. My work has yielded a straightforward, cost-effective, and high-throughput method of evaluating viability and oxygen consumption in aerobic bacteria. I have used this method to test a broad range of toxic substances, including, metal ions, antibiotics, and untransformed and transformed ENMs. I observed species specific toxic responses to Ag+, PVP-AgENMs, and sAgENMs in PGPR. These results not only show the clear utility of the methodology, but also that it will be crucial to continue examining the responses of specific bacterial strains even as nanotoxicology, as a field, must move toward more complex and environmentally relevant systems.
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Swift, Rebecca Gaye. „Novel plant growth promoting rhizobacteria (PGPR) isolated from Western Australian soils“. Thesis, Swift, Rebecca Gaye (2006) Novel plant growth promoting rhizobacteria (PGPR) isolated from Western Australian soils. Honours thesis, Murdoch University, 2006. https://researchrepository.murdoch.edu.au/id/eprint/32755/.

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Plant growth promoting rhizobacteria (PGPR) colonise plant roots and exert beneficial effects on plant growth and development. The mechanisms of action of these PGPR are not conclusively known, however, there is evidence for the role of indole-3-acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase production by rhizobacteria in plant growth promotion. In this study, novel-PGPR were isolated from the rhizosphere of native species as well as agricultural crop species, as opposed to other work in this field in which potential PGPR are isolated from the rhizosphere of the target plant species. One hundred and sixty six bacteria were isolated from four rhizosphere soils in Western Australia and 72 isolates were assayed for the production of IAA. In the presence of the auxin precursor L-tryptophan (L-TRP) IAA production ranged from 0-37 1-Lg/ml. Five rhizosphere soils were screened for bacteria capable of utilizing ACC as a sole nitrogen source and 13 isolates were obtained. To ensure that the isolates were not potentially deleterious to host plants, 14 IAA producing (IAA-PGPR) and all rhizobacteria capable of using ACC as a sole nitrogen source (ACC-PGPR) were tested for their effects on germinating clover and wheat seedlings. Two IAA-PGPR isolates, NCH7 and PMK4, were inhibitory to wheat seedling germination and one ACC-PGPR isolate was inhibitory to clover seedlings. Based on these findings, 6 IAA-PGPR and 4 ACC-PGPR were screened for their effects on germinating wheat seedlings in gnotobiotic growth pouch assays. Prior to these tests, spontaneous rifampicin resistant mutants were generated for 6 isolates. The mutants, or the wild type isolates where rifampicin mutants were not generated, were (re)tested for their ability to produce IAA and utilize ACC. All 10 isolates produced IAA in the presence of L-TRP ranging from 0.11-2.97 1-Lg IAA/ 1-Lg cellular protein and 7 of the isolates grew on ACC amended medium. Bacterial growth was greatly increased in some isolates in the L-TRP amended media used in the auxin assay, suggesting some of the isolates have a requirement for tryptophan for optimal growth. The largest increases in root lengths in the gnotobiotic growth pouch assays were observed for seed treated with thhe ACC-PGPR, AWMK3 (81% increase). The IAA-PGPR treatments that increased root lengths were PMK4R (76%), WMK10R (66%) and NCH45 (33%). Increases in shoot lengths were recorded for seed treated with isolates WMK10R (42%), AWMK3 (11%), APMK2R (9%) and PMK9 (9%). A reduction in germination was observed in seed treated with some isolates, particularly PMK4R and WMK10R, which reduced germination by 34% and 20%, respectively. Five of the PGPR isolated in this study were tested in the field on 2 wheat cultivars at 3 locations in Konjonup and Wongan Hills and as a co-inoculant with a commercial rhizobial strain on peas at Kojonup. All the PGPR were delivered in the field using the clay based A1osca™ carrier technology. The increases in yields in response to the inoculation with the PGPR on peas and wheat were small and not significantly different from the controls. However, the yield of wheat was improved by four of the PGPR (NCH45, NCH54, PMK9, WMK10) at the Wongan Hills heavy soil site by 2 to 23% and by NCH54 and PMK9 at the Wongan Hills light soil site by 4% and 3%, respectively compared with the uninoculated controls. On the peas at Kojonup, nodulation was improved with the isolate PMK4 and these plots were visually more vigorous than the other treatments, however this growth was not significant. At harvest, four of the PGPR (NCH45, PMK4, PMK9 WMK1 0) improved pea yields compared to the Alosca™ control by 6-13%. These results suggest that further testing is warranted. Improvements to experimental design and sampling have been recommended to allow for the detection of statistically significant small percentage increases if they occur. The findings in this study demonstrate that novel PGPR can be isolated from non-target as well as target plant species and that the screening of rhizobacteria based on their in vitro auxin production and growth promoting effects in growth pouch assays is valid for the selection of effective PGPR.
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Hu, Chia-Hui Kloepper Joseph. „Induction of growth promotion and stress tolerance in arabidopsis and tomato by plant growth-promoting“. Auburn, Ala., 2005. http://repo.lib.auburn.edu/2005%20Summer/doctoral/HU_CHIA-HUI_54.pdf.

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Vives, Peris Vicente. „Interaction of citrus root exudates with plant growth promoting rhizobacteria under abiotic stress conditions“. Doctoral thesis, Universitat Jaume I, 2018. http://hdl.handle.net/10803/461915.

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En la naturaleza las plantas liberan constantemente a la rizosfera una mezcla de metabolitos conocida como exudados radiculares. Su composición puede verse afectada por diferentes estímulos, incluyendo estreses abióticos como la salinidad o elevadas temperaturas. El Capítulo 1 demuestra que los portainjertos de cítricos citrange Carrizo y Citrus macrophylla exudan diferentes concentraciones de prolina y fitohormonas dependiendo del estrés abiótico y del genotipo. El Capítulo 2 estudia el efecto de dichos exudados de plantas de cítricos sometidas a salinidad y calor sobre las rizobacterias Pseudomonas putida KT2440 y Novosphingobium sp. HR1a, los cuales generalmente promueven su crecimiento. Además, se detectó la presencia de prolina y salicilatos en exudados a través del análisis de la expresión de los promotores PputA y PpahA de P. putida KT2442 y Novosphingobium sp. HR1a respectivamente. Finalmente, el Capítulo 3 muestra el efecto beneficioso de ambas bacterias en plantas de C. macrophylla sometidas a salinidad.
In nature, plants are constantly releasing a mixture of metabolites through the roots known as root exudates. Its composition can be affected by different stimuli, including abiotic stress conditions as salinity or high temperatures. Chapter 1 demonstrates that citrus rootstocks Carrizo citrange and Citrus macrophylla exude different concentrations of proline and phytohormones depending on the abiotic stress condition and the genotype. Chapter 2 studies the effect of citrus root exudates from salt- and heat-stressed plants on the rhizobacteria Pseudomonas putida KT2440 and Novosphingobium sp. HR1a, which generally promote their growth. Moreover, the presence of proline and salicylates in root exudates was also tested through the analyses of the expression of the promoters PputA and PpahA of P. putida KT2442 and Novosphingobium sp. HR1a, respectively. Finally, Chapter 3 reveals the beneficial effect of both bacterial strains in C. macrophylla plants subjected to salt stress conditions.
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Shishido, Masahiro. „Plant growth promoting rhizobacteria (PGPR) for interior spruce (Picea engelmannii x P. glauca) seedlings“. Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25159.pdf.

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Nava, Diaz Cristian. „Role of plant growth-promoting rhizobacteria in integrated disease management and productivity of tomato“. The Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=osu1135888331.

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MINAZZATO, GABRIELE. „Characterization of a transcription factor controlling vitamin B3 metabolism in plant growth promoting rhizobacteria“. Doctoral thesis, Università Politecnica delle Marche, 2020. http://hdl.handle.net/11566/274533.

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I rizobatteri sono in grado di colonizzare le radici delle piante in tutte le sue fasi di crescita, in presenza di una microflora competitiva. All'interno di questo gruppo di batteri, le specie che promuovono la crescita delle piante (PGPR) stabiliscono un'interazione benefica con le radici, migliorando la crescita e lo sviluppo dell'ospite. Evidenze sperimentali hanno dimostrato che la sintesi della forma biologicamente attiva della vitamina B3, cioè il coenzima NAD, è direttamente coinvolta negli effetti benefici mediati dai PGPR. Infatti, in Burkolderia sp. ceppo PsJN, un PGPR simbiotico della patata, l’attività dell’enzima chinolinato fosforibosiltransferasi che catalizza una reazione chiave nella via biosintetica de novo del NAD, è essenziale per promuovere la crescita della piantea. Il lavoro di questa tesi si è concentrato sullo studio della regolazione della via biosintetica de novo del NAD nei PGPR, con l'obiettivo di migliorare le nostre conoscenze sull'interazione PGPR-ospite e di ottenere nuovi strumenti in grado di migliorare lo sviluppo e il benessere delle piante. Analisi bioinformatiche hanno mostrato che nei PGPR la via de novo è controllata dal regolatore trascrizionale NadQ. Per caratterizzare questo regolatore abbiamo prodotto la proteina da Agrobacterium tumefaciens in forma ricombinante e, attraverso saggi di mobility shift, abbiamo verificato il suo legame con il DNA, in una regione situata a monte dell'operone coinvolto nelle prime fasi della biosintesi de novo del NAD. Abbiamo scoperto che NadQ lega il DNA in modo ATP e NAD-dipendente. In seguito, la risoluzione delle strutture cristallografiche del regolatore nella sua forma apo e in complesso con ATP e DNA, ha rivelato il meccanismo strutturale alla base della dissociazione della proteina dal DNA. Infine, abbiamo dimostrato che in alcune specie del genere Bordetella, NadQ regola la biosintesi de novo del NAD controllando anche il trasporto attraverso la membrana cellulare dell’acido chinolinico, precursore del NAD stesso. Abbiamo caratterizzato questo trasportatore mediante analisi di thermal shift, dimostrando la sua capacità di legare l'acido chinolinico con un’elevata affinità.
Rhizobacteria are able to colonize plant roots at all stages of plant growth, in the presence of a competing microflora. Within this group, plant growth promoting rhizobacteria (PGPRs) establish a beneficial interaction with roots, enhancing host growth and development. Experimental evidence shows that the synthesis of the biologically active form of vitamin B3, i.e. the coenzyme NAD, is directly involved in PGPRs mediated plant growth. Indeed, in Burkolderia sp. strain PsJN, a potato symbiotic PGPR, the enzyme quinolinate phosphoribosyltransferase, which catalyzes a key step in the de novo NAD biosynthetic pathway, is fundamental to promote the plant growth. Based on this evidence, this work focused on the study of the regulation of the de novo NAD biosynthetic pathway in PGPRs with the aim to enhance our knowledge on PGPR-plant interaction and to disclose novel tools to improve plant growth. Bioinformatic analyses showed that in PGPRs the pathway is controlled by the transcriptional regulator NadQ. To fully characterize this regulator, we produced the recombinant protein from A. tumefaciens and through mobility shift assays, we validated its binding to DNA, in a region upstream of the operon involved in the first steps of the de novo NAD biosynthesis. We found that NadQ binds DNA in ATP- and NAD- dependent manner. The resolution of the crystal structures of the regulator in its apo-form and in complex with ATP and DNA provided a first view of the structural mechanism of the release of the protein from DNA. Finally, we showed that in Bordetella species, NadQ regulates the de novo NAD biosynthesis by also controlling the transport of the NAD precursor quinolinic acid across the cellular membrane. We characterized the transporter by thermal shift assay, revealing its ability to bind quinolinic acid with high affinity.
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Dashti, Narjes. „Plant growth promoting rhizobacteria and soybean nodulation, and nitrogen fixation under suboptimal root zone temperatures“. Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape15/PQDD_0027/NQ29918.pdf.

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Bücher zum Thema "Promoting rhizobacteria"

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Kumar, Ashok, und Vijay Singh Meena, Hrsg. Plant Growth Promoting Rhizobacteria for Agricultural Sustainability. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-7553-8.

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Sayyed, R. Z., Hrsg. Plant Growth Promoting Rhizobacteria for Sustainable Stress Management. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6986-5.

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Sayyed, R. Z., Naveen Kumar Arora und M. S. Reddy, Hrsg. Plant Growth Promoting Rhizobacteria for Sustainable Stress Management. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6536-2.

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Egamberdieva, Dilfuza, Smriti Shrivastava und Ajit Varma, Hrsg. Plant-Growth-Promoting Rhizobacteria (PGPR) and Medicinal Plants. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-13401-7.

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Reddy, P. Parvatha. Plant Growth Promoting Rhizobacteria for Horticultural Crop Protection. New Delhi: Springer India, 2014. http://dx.doi.org/10.1007/978-81-322-1973-6.

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Sayyed, R. Z., M. S. Reddy und Sarjiya Antonius, Hrsg. Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable Agriculture. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6790-8.

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Bakker, P. A. H. M., J. M. Raaijmakers, G. Bloemberg, M. Höfte, P. Lemanceau und B. M. Cooke, Hrsg. New Perspectives and Approaches in Plant Growth-Promoting Rhizobacteria Research. Dordrecht: Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-6776-1.

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Frommel, M. Studies on a plant growth promoting rhizobacteria (PGPR): In vitro dual cultures with potato, and possible uses of its beneficial effects : potato technology project. [S.l: s.n., 1987.

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Puente, Edgar Omar Rueda. Bacterias promotoras del crecimiento vegetal. Hermosillo, Sonora, México: Universidad de Sonora, 2009.

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González, M. Belén Rodelas, und Jesús Gonzalez-López. Beneficial plant-microbial interactions: Ecology and applications. Boca Raton, FL: CRC Press, 2013.

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Buchteile zum Thema "Promoting rhizobacteria"

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Altaf, Mohd Musheer, und Mohd Sajjad Ahmad Khan. „Plant Growth Promoting Rhizobacteria“. In Microbial Biofilms, 161–74. Boca Raton : CRC Press, 2020.: CRC Press, 2020. http://dx.doi.org/10.1201/9780367415075-10.

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Reddy, P. Parvatha. „Plant Growth-Promoting Rhizobacteria (PGPR)“. In Recent advances in crop protection, 131–58. New Delhi: Springer India, 2012. http://dx.doi.org/10.1007/978-81-322-0723-8_10.

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Khan, Naeem, Asadullah und Asghari Bano. „Rhizobacteria and Abiotic Stress Management“. In Plant Growth Promoting Rhizobacteria for Sustainable Stress Management, 65–80. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6536-2_4.

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Figueiredo, Márcia do Vale Barreto, Lucy Seldin, Fabio Fernando de Araujo und Rosa de Lima Ramos Mariano. „Plant Growth Promoting Rhizobacteria: Fundamentals and Applications“. In Plant Growth and Health Promoting Bacteria, 21–43. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-13612-2_2.

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Carvalho, Thais L. G., Paulo C. G. Ferreira und Adriana S. Hemerly. „Plant Growth Promoting Rhizobacteria and Root Architecture“. In Root Genomics and Soil Interactions, 227–48. Oxford, UK: Blackwell Publishing Ltd., 2012. http://dx.doi.org/10.1002/9781118447093.ch12.

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Dwivedi, S. K., und Ram Gopal. „Sustainable Agriculture and Plant Growth Promoting Rhizobacteria“. In Microbial Diversity and Biotechnology in Food Security, 327–41. New Delhi: Springer India, 2014. http://dx.doi.org/10.1007/978-81-322-1801-2_29.

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Sumayo, Marilyn, und Sa-Youl Ghim. „Plant Growth-Promoting Rhizobacteria for Plant Immunity“. In Bacteria in Agrobiology: Crop Productivity, 329–49. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37241-4_14.

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Khalid, Azeem, Muhammad Arshad, Baby Shaharoona und Tariq Mahmood. „Plant Growth Promoting Rhizobacteria and Sustainable Agriculture“. In Microbial Strategies for Crop Improvement, 133–60. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-01979-1_7.

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Verma, Maya, Jitendra Mishra und Naveen Kumar Arora. „Plant Growth-Promoting Rhizobacteria: Diversity and Applications“. In Environmental Biotechnology: For Sustainable Future, 129–73. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-7284-0_6.

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Schoebitz, Mauricio, und María Dolores López Belchí. „Encapsulation Techniques for Plant Growth-Promoting Rhizobacteria“. In Bioformulations: for Sustainable Agriculture, 251–65. New Delhi: Springer India, 2016. http://dx.doi.org/10.1007/978-81-322-2779-3_14.

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Konferenzberichte zum Thema "Promoting rhizobacteria"

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Burygin, G. L., K. Yu Kargapolova, Yu V. Krasova und O. V. Tkachenko. „PLANT RESPONSES TO FLAGELLINS OF PLANT GROWTH-PROMOTING RHIZOBACTERIA“. In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-1203-1205.

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Aipova, R., A. Zh Abdykadyrova und A. A. Kurmanbayev. „Evaluation of the effectiveness of integrated biofertilizer in the cultivation of spring wheat in Northern Kazakhstan“. In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.008.

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Santosa, Slamet, Edi Purwanto und Sajidan Suranto. „Sustainability of Organic Agriculture System by Plant Growth Promoting Rhizobacteria (PGPR)“. In Proceedings of the International Conference on Science and Education and Technology 2018 (ISET 2018). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/iset-18.2018.92.

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Zhu, Ying, Zhiye Wang, Jianyong Wang, Zhaobin Wang und Jianping Zhou. „Plant growth-promoting rhizobacteria improve shoot morphology and photosynthesis in dryland spring wheat“. In 2013 International Conference on Biomedical Engineering and Environmental Engineering. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/icbeee130431.

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Disi, Joseph. „Plant growth promoting rhizobacteria treatment reduce oviposition by European corn borer on maize“. In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.112931.

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„Potential of ribonuclease-sinthesizing plant growth promoting rhizobacteria in plant defence against viruses“. In Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University, 2019. http://dx.doi.org/10.18699/icg-plantgen2019-24.

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Dursun, Atilla, Ertan Yildirim, Melek Ekinci, Metin Turan, Raziye Kul und Fazilet P. Karagöz. „Nitrogen fertilization and plant growth promoting rhizobacteria treatments affected amino acid content of cabbage“. In II. INTERNATIONAL CONFERENCE ON ADVANCES IN NATURAL AND APPLIED SCIENCES: ICANAS 2017. Author(s), 2017. http://dx.doi.org/10.1063/1.4981709.

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Amilia, Jumar und Tuti Heiriyani. „Peran PGPR (Plant Growth Promoting Rhizobacteria) dalam Meningkatkan Viabilitas Benih Rosella (Hibicus sabdariffa L.)“. In Seminar Nasional Semanis Tani Polije 2021. Politeknik Negeri Jember, 2021. http://dx.doi.org/10.25047/agropross.2021.221.

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Rosella merah (Hibiscus sabdariffa L.) adalah tanaman asli dari daerah yang terbentang mulai India hingga Malaysia, termasuk Indonesia. Namun di Indonesia pada kenyataannya pembudidayaan rosella merah masih terpusat di daerah-daerah tertentu seperti di pulau Jawa. Di Kalimantan Selatan, rosella mulai dikembangkan yaitu di desa Maburai Kabupaten Tabalong (laporan KKN, 2018). Mengingat manfaat rosella yang sangat baik bagi kesehatan yaitu kandungan antioksidan yang tinggi dari bunga rosella yang bisa menangkal radikal bebas dan menetralisir racun yang ada di jaringan dan sel-sel tubuh, juga menjaga kesehatan organ hati serta melawan bakteri yang masuk kedalam tubuh. Sehingga bunga rosella mulai dikembangkan untuk dijadikan produk minuman berupa sirup rosella. Untuk mendapatkan benih yang baik, daya berkecambah dan potensi tumbuh yang tinggi diperlukan teknologi perlakuan untuk menigkatkan viabilitas benih seragam dan bermutu. Penggunaan mikroorganisme rhizobakteri atau dikenal sebagai PGPR (Plant Growth Promoting Rhizobacteria) dapat memberikan daya kecambah dan percepatan tumbuh rosella. Penelitian ini bertujuan untuk mengetahui interaksi perendaman dengan konsentrasi PGPR yang berbeda untuk mendapatkan viabilias yang terbaik. Rancangan yang digunakan adalah Acak Lengkap (RAL) dua faktor. Faktor pertama adalah konsentrasi PGPR yang terdiri dari KNO3 20 g.l-1 (k0), PGPR 5 ml.l-1 (k1), PGPR 10 ml.l-1 (k2) dan PGPR 15 ml.l-1 (k3). Faktor kedua adalah lama perendaman yaitu 8 jam (l1), 12 jam (l2) serta 24 jam (l3), perlakuan diulang sebanyak 3 kali. Hasil penelitian menunjukkan bahwa konsentrasi PGPR dan perendaman terbaik dalam meningkatkan viabiltas benih rosella adalah pada perlakuan konsentrasi PGPR 5 ml dengan lama perendaman 8 jam (k1l1) dimana menghasilkan potensi tumbuh maksimum sebesar 85,33%.
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„Plant Growth-Promoting Rhizobacteria Improved Seedling Growth and Quality of Cucumber (Cucumis sativus L.)“. In International Conference on Chemical, Food and Environment Engineering. International Academy Of Arts, Science & Technology, 2015. http://dx.doi.org/10.17758/iaast.a0115068.

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Ji, Yun-Xiu, und Xiao-Dong Huang. „Amelioration of Salt Stress on Annual Ryegrass by ACC Deaminase-Containing Plant Growth-Promoting Rhizobacteria“. In 2008 2nd International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2008. http://dx.doi.org/10.1109/icbbe.2008.527.

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Berichte der Organisationen zum Thema "Promoting rhizobacteria"

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Crowley, David E., Dror Minz und Yitzhak Hadar. Shaping Plant Beneficial Rhizosphere Communities. United States Department of Agriculture, Juli 2013. http://dx.doi.org/10.32747/2013.7594387.bard.

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PGPR bacteria include taxonomically diverse bacterial species that function for improving plant mineral nutrition, stress tolerance, and disease suppression. A number of PGPR are being developed and commercialized as soil and seed inoculants, but to date, their interactions with resident bacterial populations are still poorly understood, and-almost nothing is known about the effects of soil management practices on their population size and activities. To this end, the original objectives of this research project were: 1) To examine microbial community interactions with plant-growth-promoting rhizobacteria (PGPR) and their plant hosts. 2) To explore the factors that affect PGPR population size and activity on plant root surfaces. In our original proposal, we initially prqposed the use oflow-resolution methods mainly involving the use of PCR-DGGE and PLFA profiles of community structure. However, early in the project we recognized that the methods for studying soil microbial communities were undergoing an exponential leap forward to much more high resolution methods using high-throughput sequencing. The application of these methods for studies on rhizosphere ecology thus became a central theme in these research project. Other related research by the US team focused on identifying PGPR bacterial strains and examining their effective population si~es that are required to enhance plant growth and on developing a simulation model that examines the process of root colonization. As summarized in the following report, we characterized the rhizosphere microbiome of four host plant species to determine the impact of the host (host signature effect) on resident versus active communities. Results of our studies showed a distinct plant host specific signature among wheat, maize, tomato and cucumber, based on the following three parameters: (I) each plant promoted the activity of a unique suite of soil bacterial populations; (2) significant variations were observed in the number and the degree of dominance of active populations; and (3)the level of contribution of active (rRNA-based) populations to the resident (DNA-based) community profiles. In the rhizoplane of all four plants a significant reduction of diversity was observed, relative to the bulk soil. Moreover, an increase in DNA-RNA correspondence indicated higher representation of active bacterial populations in the residing rhizoplane community. This research demonstrates that the host plant determines the bacterial community composition in its immediate vicinity, especially with respect to the active populations. Based on the studies from the US team, we suggest that the effective population size PGPR should be maintained at approximately 105 cells per gram of rhizosphere soil in the zone of elongation to obtain plant growth promotion effects, but emphasize that it is critical to also consider differences in the activity based on DNA-RNA correspondence. The results ofthis research provide fundamental new insight into the composition ofthe bacterial communities associated with plant roots, and the factors that affect their abundance and activity on root surfaces. Virtually all PGPR are multifunctional and may be expected to have diverse levels of activity with respect to production of plant growth hormones (regulation of root growth and architecture), suppression of stress ethylene (increased tolerance to drought and salinity), production of siderophores and antibiotics (disease suppression), and solubilization of phosphorus. The application of transcriptome methods pioneered in our research will ultimately lead to better understanding of how management practices such as use of compost and soil inoculants can be used to improve plant yields, stress tolerance, and disease resistance. As we look to the future, the use of metagenomic techniques combined with quantitative methods including microarrays, and quantitative peR methods that target specific genes should allow us to better classify, monitor, and manage the plant rhizosphere to improve crop yields in agricultural ecosystems. In addition, expression of several genes in rhizospheres of both cucumber and whet roots were identified, including mostly housekeeping genes. Denitrification, chemotaxis and motility genes were preferentially expressed in wheat while in cucumber roots bacterial genes involved in catalase, a large set of polysaccharide degradation and assimilatory sulfate reduction genes were preferentially expressed.
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