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Zeitschriftenartikel zum Thema „Production des fibres de lin“

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Veröffentlicht am 18. Mai 2024

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1

CHILLIARD, Y., A. FERLAY und M. DOREAU. „Contrôle de la qualité nutritionnelle des matières grasses du lait par l’alimentation des vaches laitières : acides gras trans, polyinsaturés, acide linoléique conjugué“. INRAE Productions Animales 14, Nr. 5 (17.12.2001): 323–35. http://dx.doi.org/10.20870/productions-animales.2001.14.5.3758.

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Après un rappel des voies métaboliques et des flux de nutriments qui concourent à la lipogenèse mammaire, cet article est consacré aux principaux effets de l’alimentation sur la composition en acides gras (AG) du lait de ruminant, en particulier les AG polyinsaturés, les AG monoinsaturés trans et l’acide linoléique conjugué (CLA). Les principaux facteurs alimentaires étudiés sont la nature des fourrages (dont l’herbe pâturée), le suif, protégé ou non, et la supplémentation des rations avec des huiles végétales ou marines (poisson ou algues), protégées ou non. La supplémentation en suif augmente la sécrétion d’acide oléique. La teneur du lait en C18:2 est comprise entre 2 et 3 % avec les rations non supplémentées en lipides, elle n’est augmentée que de 1,5 point lors d’un apport de graines ou d’huiles riches en C18:2, en raison d’une hydrogénation ruminale poussée. Les rations à base d’herbe (riches en C18:3) augmentent légèrement (0,5 à 1 point) la proportion de C18:3 dans le lait par rapport aux rations à base d’ensilage de maïs. La graine de lin permet un accroissement d’environ 0,3 point. La teneur en C20:5 (EPA) et en C22:6 (DHA) est accrue jusqu’à 0,5 % des AG totaux par l’addition d’huiles marines à la ration. Le taux butyreux du lait peut être fortement diminué par les régimes pauvres en fibres et riches en céréales et/ou par l’administration d’huiles marines ou végétales riches en AG insaturés. Les C18:1 trans, notamment les C18:1 trans 11 et trans 10, mais aussi le trans 10, cis 12 CLA, pourraient jouer un rôle dans cette diminution en inhibant la lipogenèse mammaire. Les régimes alimentaires augmentant les proportions des CLA et des C18:1 trans dans le lait sont ceux qui apportent des précurseurs lipidiques pour la formation de ces AG, ou qui modifient l’activité microbienne associée à l’hydrogénation ruminale des AG polyinsaturés ou l’activité de la désaturase mammaire. L’influence de l’alimentation sur les différents isomères du C18:1 et du CLA du lait a été peu étudiée.

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Okine, E. K., G. W. Mathison und R. T. Hardin. „Relations between passage rates of rumen fluid and particulate matter and foam production in rumen contents of cattle fed on different diets ad lib.“ British Journal of Nutrition 61, Nr. 2 (März 1989): 387–95. http://dx.doi.org/10.1079/bjn19890125.

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1. A group of six cattle, three of which had a non-bloating history (group A) and had been ruminally cannulated for the previous 2 years, and three with a history of being bloat-prone (group B) and which had been ruminally cannulated only 3 months before the study, were fed ad lib. on chopped lucerne (Medicago sotiva) hay, lucerne pellets, or a 100 g chopped hay and 900 g rolled barley grain/kg diet over three periods of 30 d each. Flow of rumen digesta, by reference to CoEDTA and chromium-mordanted fibres, and foam production from samples of rumen contents were measured.2. Samples of rumen contents (50 ml) from group A produced foam heights of 150 and 60 mm, 2 and 4 h after feeding respectively, compared with 240 and 150 mm for group B (P < 0.05).3. The fractional passage rate of the 1-2 mm particles mordanted with Cr did not differ (P > 0.05) between groups.4. The fractional outflow rates (FOR) for CoEDTA 0-2 h and 2-7 h after feed was offered were 0.205 and 0.160/h for group A and 0.093 and 0.086/h for group B respectively (P < 0.05).5. Rumen-fluid FOR 0-2 h and 2-7 h after provision of feed were significantly (P < 0.05) inversely correlated (r -0.74 and -0.85 respectively) with the amount of foam produced from rumen contents at these times.

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Paranagama, Iranga, Indira Wickramasinghe, Dulani Somendrika und Kasun Benaragama. „DEVELOPMENT OF A VEGAN SAUSAGE WITH YOUNG GREEN JACKFRUIT, OYSTER MUSHROOM, AND COCONUT FLOUR AS AN ENVIRONMENTALLY FRIENDLY PRODUCT WITH CLEANER PRODUCTION APPROACH“. Journal of microbiology, biotechnology and food sciences 11, Nr. 4 (01.02.2022): e4029. http://dx.doi.org/10.55251/jmbfs.4029.

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Veganism or vegetarianism is a trending topic in the modern world. Natural disasters, climate change, and pandemics lead people towards healthier and plant-based diets and nature-friendly lifestyle. Higher meat consumption has led the world into a huge struggle resulting in global warming, rising sea levels, disease spreading like swine flu, bird flu, coronavirus, etc. The main objective of this research was to develop a socially responsible vegan product, minimizing its environmental impact with a cleaner production approach. Generally, many vegan products are produced using soy as the main ingredient, but it is the second-largest agricultural driver of deforestation globally. Currently, there are no significant studies done on vegan product development considering the environmental impact and cleaner production. Concerning all these objectives, this vegan sausage was developed with young green jackfruit (Artocarpus heterophyllus), oyster mushrooms (Pleurotus ostreatus), and coconut flour (Cocos nucifera Lin.) as main ingredients. This product was subjected to sensory evaluation, proximate analysis using AOAC and SLS methods, and microbiological analysis. The moisture, ash, total fat, crude protein, and crude fibre contents of the product were 64.49 ± 0.16%, 2.62 ± 0.18%, 5.94 ± 0.25%, 3.95 ± 0.30% and 1.22 ± 0.16% respectively. Total carbohydrate value and energy profiles were 21.78 ± 0.38% and 156.3 kcal/100 g calculated according to the Codex Guideline on Nutrition Labelling. Following a cleaner production approach helped to identify that it is more environmentally friendly using electricity than LPG in Sri Lanka for this kind of product development and to reduce the total CO2 emission per kg of vegan sausage from 0.678 kg CO2/kg to 0.477 kg CO2/kg.

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Thuault, A., S. Eve, J. Bazin, K. Charlet, F. Destaing, M. Gomina und J. Bréard. „Morphologie, biocomposition et comportement mécanique des fibres de lin“. Matériaux & Techniques 99, Nr. 3 (2011): 275–80. http://dx.doi.org/10.1051/mattech/2011011.

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5

Riedt, Tamara, Steven Goossens, Ines Gütgemann, Carmen Carrillo-Garcia, Hichem D. Gallala, Holger Fröhlich, Peter Brossart, Dany Huylebroeck, Jody J. Haigh und Viktor Janzen. „Zeb2-Defficiency in the Adult Murine Hematopoietic Precursor Cells Leads to Differentiation Defects in Multiple Hematopoietic Lineages and a Myeloproliferative-Like Phenotype“. Blood 120, Nr. 21 (16.11.2012): 1199. http://dx.doi.org/10.1182/blood.v120.21.1199.1199.

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Abstract Abstract 1199 The life long replenishment of highly specialized blood cells by a small number of hematopoietic cells (HSC) requires a strict regulation between self-renewal and differentiation in the immature compartment of the bone marrow. Perturbation of this equilibrium can result in stem cell loss or hematologic malignancies. This balance is at least in part controlled by a network of transcription factors. Zeb2 is a transcriptional repressor and plays an important role during the embryonic development as a modulator of the epithelial to mesenchymal transition (EMT) as well as tumor progression and metastasis. We have previously identified the essential role of Zeb2 in murine embryonic hematopoiesis, where selective Zeb2 deficiency in the hematopoietic stem cells resulted in early lethality around day 12.5. The aim of this study was to analyze whether Zeb2 plays a specific role in the regulation of homeostasis in the adult hematopoietic system. Using the Mx1-Cre based inducible Zeb2 conditional knock out mouse model we analyzed the impact of Zeb2 loss on adult hematopoietic stem cell function. Upon the induction of Zeb2 deletion we found a significant decrease in most cell lineages of the peripheral blood, except the neutrophil granulocytes. However, the reduction of mature cells in the blood was not accompanied by reduced bone marrow cellularity, as the cellularity was similar between Zeb2Δ/Δ Mx1-Cre (Zeb2 conditional KO) mice and the control animals (Zeb2+/+Mx1-Cre). However, in the bone marrow of the Zeb2Δ/Δ Mx1-Cre animals the granulocytic lineage was dominating, whereas other lineages e.g. red blood cell precursors and B-lymphoid precursors were drastically reduced. Histological sections of the bone marrow cavity revealed megacaryocytes with abnormal morphology reflecting maturation defects and an increased production of reticular fibers in the BM of Zeb2Δ/Δ Mx1-Cre mice. In addition Zeb2Δ/Δ Mx1-Cre mice displayed a two to three fold increase in spleen size compared to control animals due to an extramedullary hematopoiesis. Analysis of the primitive hematopoietic compartment in the bone marrow and spleens revealed that Zeb2 deletion resulted in a pronounced increase in the most immature hematopoietic cells, defined as Lin-Sca1+cKit+CD48-CD150+ population, and perturbation in different lineage restricted progenitor subpopulations. No difference in cell cycling or apoptotic rate in the stem cell enriched bone marrow population (Lin-Sca1+cKit+CD48-CD150+) was detectable between the genotypes. Upon transplantation into lethally irradiated wild type recipients, Zeb2 deficient stem cells demonstrated significantly reduced ability to differentiate into multiple hematopoietic lineages indicating a niche independent effect of Zeb2 in promoting differentiation of hematopoietic stem cells. On the molecular level, gene expression analysis of hematopoietic stem and progenitor cells using microarray approach revealed increased transcripts of downstream targets of Wnt/ß-Catenin signaling, suggesting increased Wnt signaling activity in absence of Zeb2 in the hematopoietic compartment, which at least in part might be responsible for the observed phenotype. These data indicate that Zeb2 is involved in the regulation of the balance between self-renewal and differentiation at multiple stages of hematopoietic cell maturation. Furthermore the lack of Zeb2 in the hematopoietic compartment leads to a phenotype that resembles the features of human myeloproliferative disorders, especially the early stages of primary myelofibrosis with dominant granulopoiesis, production of reticular fibers in the bone marrow, and morphological abnormalities in megacaryocytes, accompanied by extramedullary hematopoiesis. Disclosures: No relevant conflicts of interest to declare.

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LANGFELD, KAREN S., JOHN D. ALTRINGHAM und IAN A. JOHNSTON. „Temperature and the Force-Velocity Relationship of Live Muscle Fibres from the Teleost Myoxocephalus Scorpius“. Journal of Experimental Biology 144, Nr. 1 (01.07.1989): 437–48. http://dx.doi.org/10.1242/jeb.144.1.437.

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Small bundles of fast fibres were isolated from the myotomal muscle of the teleost Myoxocephalus scorpius. The temperature-dependence of isometric contractile properties and the force-velocity (P-V) relationship were studied. Fibres were found to deteriorate above 18°C, and the force plateau during tetanic stimulation was not maintained above 15°C. Twitch and tetanic tension (P0) showed optima at around 8°C. Force-velocity curves were fitted using either Hill's hyperbolic equation or a hyperbolic-linear equation (hyp-lin). The best fit to the data was provided by the hyp-lin equation, which gave consistently higher values for unloaded contraction velocity (Vmax): 4.3, 8.1 and 9.5 muscle lengths s−1 at 1, 8 and 12°C, respectively. The P-V relationship was found to become progressively more curved at higher temperatures. Muscle power output calculated from the hyp-lin equation was 123 W kg−1 at 1°C and 256 W kg−1 at 8°C. Curves normalized for P0 and Vmax at each temperature show that the change in curvature is sufficient to increase the relative power output of the muscle by around 15% on decreasing the temperature from 8 to 1°C.

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Martin, Nicolas, Peter Davies und Christophe Baley. „Influence du rouissage du lin sur les propriétés mécaniques des fibres et des composites injectés lin/polypropylène“. Revue des composites et des matériaux avancés 24, Nr. 1 (30.04.2014): 139–53. http://dx.doi.org/10.3166/rcma.24.139-153.

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8

Wang Chunxia, 王春霞, und 刘云朋 Liu Yunpeng. „基于光纤传感的工业生产线智能装配系统“. Infrared and Laser Engineering 51, Nr. 10 (2022): 20210695. http://dx.doi.org/10.3788/irla20210695.

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9

Cherf, Zine Eddine, Christophe Poilane, Ladan Moyamez und Jun Chen. „Optimisation d'un préimprégné lin/époxy industriel. Influence de l'orientation des fibres“. Revue des composites et des matériaux avancés 21, Nr. 1 (April 2011): 119–28. http://dx.doi.org/10.3166/rcma.21.119-128.

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10

Cheour, Khouloud, Mustapha Assarar, Daniel Scida, Rezak Ayad und Xiao-Lu Gong. „Identification des coefficients d’amortissement de matériaux composites à fibres de lin“. Revue des composites et des matériaux avancés 26, Nr. 3-4 (30.12.2016): 367–82. http://dx.doi.org/10.3166/rcma.26.367-382.

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11

Bono, Pierre, Anne Le Duc, Marie Lozachmeur und Arnaud Day. „Matériaux : les nouveaux champs de recherche et développement pour la valorisation des fibres végétales techniques (lin fibres et chanvre)“. OCL 22, Nr. 6 (02.10.2015): D613. http://dx.doi.org/10.1051/ocl/2015041.

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Baksheev, I. P., und P. A. Butyagin. „World production of viscose fibres“. Fibre Chemistry 29, Nr. 4 (Juli 1997): 221–24. http://dx.doi.org/10.1007/bf02430715.

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13

Tung, L. H., F. Khadraoui, M. Boutouil und M. Gomina. „Caractérisation microstructurale et mécanique d’un composite cimentaire renforcé par des fibres de lin“. MATEC Web of Conferences 2 (2012): 01014. http://dx.doi.org/10.1051/matecconf/20120201014.

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Reinhart, Brenda J., und Gary Ruvkun. „Isoform-Specific Mutations in the Caenorhabditis elegans Heterochronic Gene lin-14 Affect Stage-Specific Patterning“. Genetics 157, Nr. 1 (01.01.2001): 199–209. http://dx.doi.org/10.1093/genetics/157.1.199.

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Abstract The Caenorhabditis elegans heterochronic gene lin-14 specifies the temporal sequence of postembryonic developmental events. lin-14, which encodes differentially spliced LIN-14A and LIN-14B1/B2 protein isoforms, acts at distinct times during the first larval stage to specify first and second larval stage-specific cell lineages. Proposed models for the molecular basis of these two lin-14 gene activities have included the production of functionally distinct isoforms and the generation of a temporal gradient of LIN-14 protein. We report here that loss of the LIN-14B1/B2 isoforms alone affects one of the two lin-14 temporal patterning functions, the specification of second larval stage lineages. A temporal expression difference between LIN-14A and LIN-14B1/B2 is not responsible for the stage-specific phenotype: protein levels of all LIN-14 isoforms are high in early first larval stage animals and decrease during the first larval stage. However, LIN-14A can partially substitute for LIN-14B1/B2 when expressed at a higher-than-normal level in the late L1 stage. These data indicate that LIN-14B1/B2 isoforms do not provide a distinct function of the lin-14 locus in developmental timing but rather may contribute to an overall level of LIN-14 protein that is the critical determinant of temporal cell fate.

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DE PALMENAER, Andreas. „Exergetic Analysis of the Thermal Conversion Process in PAN-Based Carbon Fibre Production“. Tekstil ve Mühendis 22, Nr. 97 (31.03.2015): 7–13. http://dx.doi.org/10.7216/130075992015229702.

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16

Sretenovic, Lj, V. Pantelic, M. Novakovic, Z. Novakovic und D. Ostojic-Andric. „Production of beef meat with functional food roperties“. Biotehnologija u stocarstvu 27, Nr. 3 (2011): 373–85. http://dx.doi.org/10.2298/bah1103373s.

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In this study the effects of ?Tradi-Lin? as a source of omega-3 fatty acids (FA) in diets for fattening cattle were presented. ?Tradi-Lin? produced from flax seed had 58% omega-3 and 15% omega-6 fatty acids of total polyunsaturated fatty acids (PUFA). Trial was carried out on three groups of finishing beef cattle (C, T1 and T2) with 15 heads in each and was lasted 60 days. The diets used based on maize silage and concentrate mixture. Cattle received 0, 300 and 700 g of ?Tradi-Lin? per day, respectively. At the end of trial heads were slaughtered and major parameters of the chemical composition and meat quality were determined. The results indicated that were no differences in saturated (50.85; 50.21; 50.34%) and unsaturated (49.15; 49.79; 49.66%) FA between treatments in intramuscular fat of the m. longissimus dorsi but ?Tradi-Lin? influenced the changes in structure of intramuscular fat and increased PUFA from 4.91 (C) to 5.54 (T1) and 7.31% (T2) ( P< 0.05). The increase of omega-3 FA from 0.36 (C) to 0.60 (T1) and 0.76% (T2) must be pointed out, ( P< 0.05). The omega-6 FA were also increased from 4.51 (C) to 4.94 (T1) and 6.22% (T2) (P>0.05) but omega-6: omega- 3 ratio was reduced from 12.25 (C) to 8.22 (T1) (P< 0.05) and 8.62(T2), (P<0.01). Trans unsaturated FA which are undesirable were decreased from 3.37% (C) to 2.43% (T1) and 2.36% (T2)(P<0.05). From nutritional aspect those results have a great importance, having in mind that omega-3 FA have a vital role in human health.

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Lee, Youngae, Yutaro Kumagai, Shizuo Akira und Myoung Ho Jang. „Intestinal mast cell progenitors function as innate lymphoid cells (P497) (71.3)“. Journal of Immunology 188, Nr. 1_Supplement (01.05.2012): 71.3. http://dx.doi.org/10.4049/jimmunol.188.supp.71.3.

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Abstract Small intestinal innate lymphoid cells (mainly NKp46+ILCs) are known to have a protective role from infection of pathogenic bacteria through the production of IL-22. The proportions of ILCs localized intestinal lamina propria are higher than those in the others lymphoid tissues, such as mesenteric lymph node, spleen, liver, and bone marrow. In this study, we did a microarray experiment to study whether small intestinal ILCs (Lin-c-Kit+Sca-1- cells) highly express which genes as compared with non-ILCs (Lin-c-Kit-Sca-1- cells). We selected 251 up-regulated genes and 219 down-regulated genes in Lin-c-Kit+Sca-1- cells. Especially, Lin-c-Kit+Sca-1- cells highly expressed genes coding to Il22, Csf2rb2, mast cell proteases (Mcpts), and Rorc. To address whether which ILC populations are main source of IL-22, we more detailed divided Lin-c-Kit+Sca-1- cells into the two groups [Lin-c-Kit+NKp46+ (NKp46+ILCs) and Lin-c-Kit+NKp46-CD4-]. Only Lin-c-Kit+NKp46-CD4- cells expressed Csf2rb2 and Mcpts transcripts. These cells expressed higher level of Il22 mRNA, IL-22 protein, and IL-17F protein in physiological condition and in IL-23 stimulated condition compared with NKp46+ILCs. Moreover, these cells could differentiate into mast cells in the presence of mIL-3 and mSCF. Taken together, Lin-c-Kit+NKp46-CD4- cells as mast cell progenitors may regulate small intestinal homeostasis through the production of Mcpts, IL-17F, and IL-22 in physiological condition and in pathophysiological condition.

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Radishevskii, M. B., A. V. Kalacheva und A. T. Serkov. „Semicontinuous Production of Viscose Textile Fibres“. Fibre Chemistry 35, Nr. 6 (November 2003): 426–28. http://dx.doi.org/10.1023/b:fich.0000020771.40076.3d.

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Moro, Kazuyo, und Shigeo Koyasu. „Innate production of Th2 cytokines by adipose tissue-associated c-Kit+Sca-1+ lymphoid cells (89.11)“. Journal of Immunology 184, Nr. 1_Supplement (01.04.2010): 89.11. http://dx.doi.org/10.4049/jimmunol.184.supp.89.11.

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Abstract Innate immune responses are important in combating various microbes during the early phases of infection. NK cells are innate lymphocytes that, unlike T and B lymphocytes, do not express antigen receptors but rapidly exhibit cytotoxic activities against virus-infected cells and produce various cytokines. Here we report a new type of innate lymphocyte present in a novel lymphoid structure associated with adipose tissues in the peritoneal cavity. These cells do not express lineage (Lin) markers but do express c-Kit, Sca-1, IL7R and IL33R. Similar lymphoid clusters were found in both human and mouse mesentery and we term this tissue ‘FALC’ (fat-associated lymphoid cluster). FALC Lin-c-Kit+Sca-1+ cells are distinct from lymphoid progenitors and lymphoid tissue inducer cells. These cells proliferate in response to IL2 and produce large amounts of Th2 cytokines such as IL5, IL6 and IL13. IL5 and IL6 regulate B-cell antibody production and self-renewal of B1 cells. Indeed, FALC Lin-c-Kit+Sca-1+ cells support the self-renewal of B1 cells and enhance IgA production. After helminth infection and in response to IL33, FALC Lin-c-Kit+Sca-1+ cells produce large amounts of IL13, which leads to goblet cell hyperplasia—a critical step for helminth expulsion. In mice devoid of FALC Lin-c-Kit+Sca-1+ cells, such goblet cell hyperplasia was not induced. Thus, FALC Lin-c-Kit+Sca-1+ cells are Th2-type innate lymphocytes, and we propose that these cells be called ‘natural helper cells’.

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20

Grohens, Yves, Claude Charreteur, Jérémie Goimard und Christophe Baley. „Influence des intrants agricoles sur l'écobilan des fibres de lin, renforts de matériaux composites“. Revue des composites et des matériaux avancés 18, Nr. 2 (31.08.2008): 245–50. http://dx.doi.org/10.3166/rcma.18.245-250.

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21

Charlet, K., J. P. Jernot, M. Gomina, S. Eve und J. Bréard. „Corrélations entre les propriétés mécaniques des fibres de lin et celles des composites dérivés“. Matériaux & Techniques 99, Nr. 3 (2011): 317–25. http://dx.doi.org/10.1051/mattech/2011046.

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Kuznetsova, Svetlana. „How to create an efficient production system in an enterprise“. Normirovanie i oplata truda v promyshlennosti (Rationing and remuneration of labor in industry), Nr. 8 (01.08.2020): 24–32. http://dx.doi.org/10.33920/pro-3-2008-02.

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What is the difference between the production philosophy of a Japanese leader and approaches to enterprise management in Russia? Are Lean Methods the “panacea” that will improve production efficiency in any enterprise? Where should you start transformations and what should you pay special attention to when starting to improve the production system? Finally, what difficulties do Russian companies face when implementing LIN technologies? Answers to these and other no less pressing questions – in an interview with Nikolay Rozenko, Director for Development and Projects, Lead Trainer of LIN-Systems.

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Komnick, Kristin, Jennifer May, Pouneh Kermani, Sreemanti Basu, Irene Hernandez, Mark Zogg, British L. Fields, Joshua Morris, Hartmut Weiler und Karen-Sue Carlson. „Dependence on Schwann Cell-Derived Laminin-γ1 Differentiates Early Lymphoid and Myeloid Development“. Blood 136, Supplement 1 (05.11.2020): 37. http://dx.doi.org/10.1182/blood-2020-142860.

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Blood cell production is regulated by peripheral nerve fibers that innervate the bone marrow. However, little is known about the development or maintenance of hematopoietic innervation. Schwann cells (SCs) are the primary axon 'support cells' of the peripheral nervous system (PNS), and abnormal SC development is sufficient to impair peripheral nerve function. SCs are also the primary repair cell for the PNS which makes them an attractive therapeutic target for normalization of drug or malignancy-induced 'hematopoietic neuropathy'. We hypothesized that neural regulation of hematopoiesis is dependent on SC development. To test this hypothesis, we used the Myelin Protein Zero-Cre (MP0-Cre); Lamc1fl/fl mouse line in which laminin-γ1 expression is deleted from SC precursors and their progeny1. Early SC maturation is dependent on autocrine SC precursor-derived molecules such as laminin-γ1. SC differentiation arrests prior to axon sorting and ensheathment in MP0-Cre; Lamc1fl/fl mice, and causes a global peripheral neuropathy that persists throughout the lifetime of the animal. Preliminary hematopoietic analysis of 'steady state' MP0-Cre; Lamc1fl/fl and littermate control mice has shown the following: (1) MP0-Cre; Lamc1fl/fl bone marrow is innervated, and Cre-mediated gene recombination occurs in cells immunophenotypically consistent with SCs throughout the peripheral nervous system, including those in the bone marrow; (2) MP0-Cre; Lamc1fl/fl mice are lymphopenic but not neutropenic; (3) MP0-Cre; Lamc1fl/fl mice have significantly reduced spleen size and cellularity; and (4) MP0-Cre; Lamc1fl/fl bone marrow has an ~50% reduction in Lin-Sca-1+Kit+(LSK) cells (measured as a percentage of the Lin- compartment of the bone marrow). These results are consistent with earlier work by our groups in which we found that global Lamc1 gene deletion in adult mice induced peripheral blood lymphopenia, reduced spleen size, and a niche-dependent reduction of lymphoid progenitor and precursor cells that was secondary to increased lymphoid precursor cell apoptosis and reduced proliferation (UBC-CreERT2; Lamc1fl/fl mouse line). As with the SC-specific laminin-γ1 deficient mice, myelopoiesis was preserved in the UBC-CreERT2; Lamc1fl/fl mice. Based on results from MP0-Cre; Lamc1fl/fl and UBC-CreERT2; Lamc1fl/fl mice, we conclude that early lymphoid but not myeloid development requires laminin-γ1 expression by MP0-Cre-targetted niche cells, i.e. Schwann Cells. Our results are consistent with reports from other labs that hematopoietic sympathetic neuropathy promotes aberrant myeloid expansion at the expense of lymphopoiesis2. Going forward, we will determine whether lymphopoietic development is dependent on global versus laminin-specific SC-derived cues, and whether these signals are transmitted directly between SCs and lymphoid biased HSPCs or indirectly via other components of the hematopoietic niche. We anticipate that this line of investigation will provide molecular insights and pharmacologic targets for prevention and or normalization of the 'hematopoietic neuropathy' induced by diabetes, aging, neurotoxic chemotherapies and myeloid malignancies. REFERENCES: 1 Yu, W. M., Feltri, M. L., Wrabetz, L., Strickland, S. & Chen, Z. L. Schwann cell-specific ablation of laminin gamma1 causes apoptosis and prevents proliferation. J Neurosci25, 4463-4472, doi:10.1523/JNEUROSCI.5032-04.2005 (2005). 2 Maryanovich, M. et al. Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche. Nat Med24, 782-791, doi:10.1038/s41591-018-0030-x (2018). Disclosures No relevant conflicts of interest to declare.

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Sogo, Shinji, Kuniko Matsumura-Takeda, Yoshimasa Isakari, Yasuo Harada, Kinue Nishioka, Takuma Kawakami, Toshihide Ono und Takao Taki. „Identification of New Megakaryocytic Subpopulations in Mouse Bone Marrow.“ Blood 106, Nr. 11 (16.11.2005): 2265. http://dx.doi.org/10.1182/blood.v106.11.2265.2265.

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Abstract Platelets (PLT) are produced from megakaryocytes (Mks) via proplatelet formation (PPF). However, the molecular mechanisms from Mks to PPF are not clearly elucidated, because the maturational steps of the Mks in bone marrow (BM) are not analyzed in detail. Until now, mouse Mks have been only isolated as acetylcholinesterase (AchE) positive cells and they are understood as well maturated population. In this study, we found the presence of different megakaryocytic subpopulations in BM by flowcytometry. To isolate the Mks, first we depleted lineage marker (CD4, CD8a, CD11b, B220, CD71, CD90, TER119, Gr-1, F4/80, 7/4) positive cells from BM cells of BALB/c mice. The analysis of the expression-pattern of CD41, CD45 and CD61 in the lineage negative (Lin−) cells showed the presence of two types of megakaryocytic subpopulations. By sorting, they were identified as Lin−CD41+/45+/61+ cells (AchE negative) and Lin−CD41++/45+/61++ cells (partially AchE positive), respectively. To assess the maturational stages of the subpopulations, each population was cultured with 10ng/mL of TPO followed by counting of PPF and PLT production. Both PPF and PLT production were observed in Lin−CD41+/45+/61+ cells later than those in Lin−CD41++/45+/61++ cells. On the other hand, CFU-Mk was scarcely detected in each subpopulation. The results indicate that both populations are the committed megakaryocytes and Lin−CD41+/45+/61+ cells are more immature population than Lin−CD41++/45+/61++ cells. Then to characterize these subpopulations in detail, gene expression profiling was performed against four-megakaryocytic lineage-populations, Lin−CD41−Thy1lowc-kit+ cells as stem/progenitor, Lin−CD41+/45+/61+ cells, Lin−CD41++/45+/61++ cells and PLT using GeneChipU74 or RT-PCR. These analyses revealed that many PLT-specific genes including gpIb/IX, P-selectin, thrombin-R and ADP-R were already expressed on Lin−CD41+/45+/61+ cells but less than Lin−CD41++/45+/61++ cells. Especially, beta-1 tubulin that is necessary for PPF was only expressed on Lin−CD41++/45+/61++ cells. On the contrary, the expression of c-kit gene was gradually decreasing from stem/progenitor fraction to PLT. In conclusion, we succeeded in the isolation of new subpopulations distinguishable between immature Mks and more matured Mks beginning to prepare PLT. The present finding can contribute to elucidate the molecular mechanisms during terminal maturation.

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Morvan, O., A. Jauneau, C. Morvan, H. Voreux und M. Demarty. „Biosynthèse des pectines et différenciation des fibres cellulosiques au cours de la croissance du lin“. Canadian Journal of Botany 67, Nr. 1 (01.01.1989): 135–39. http://dx.doi.org/10.1139/b89-020.

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During the first stage of flax growth, stem elongation reaches 2.4 cm per day and the percentage of cell wall remains quite constant (4–15%). Cellulosic fibres develop principally during capsule formation and seed maturation. During the latter stage, the proportion of walls increases from 15 to 60% and the elongation is diminished to 0.5 cm per day. The lowering of the cation exchange capacity and of the pectin content of the cell walls during growth results principally from increased cellulose deposition in the fibre cells. The changes in the cation exchange capacity and in the percentage of cell wall show that when cellulose biosynthesis predominates, there is a continuous synthesis of pectins (10–15%) during the development of the plant. Methylated pectins are synthesized during the elongation phase. During maturation, the relative amounts of highly and less methylated pectins remain the same and thus it is not possible to determine what type of pectin is preferentially synthesized.

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Zhang, Xiaoli, Zhen He und Liangxing Shi. „Process Quality Metrics for Mechanical and Electrical Production Lin“. Procedia Engineering 24 (2011): 6–11. http://dx.doi.org/10.1016/j.proeng.2011.11.2592.

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BRUNSCHWIG, P., C. HURTAUD, Y. CHILLIARD und F. GLASSER. „L’apport de lin dans la ration des vaches laitières : Effets sur la production, la composition du lait et des produits laitiers, les émissions de méthane et les performances de reproduction“. INRAE Productions Animales 23, Nr. 4 (14.11.2010): 307–18. http://dx.doi.org/10.20870/productions-animales.2010.23.4.3310.

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La supplémentation en lin des rations des vaches laitières est une pratique qui se développe, avec pour objectifs l’amélioration de la qualité nutritionnelle du lait et la diminution des rejets de méthane. En effet, la recherche de laits moins riches en Acides Gras (AG) saturés et avec un rapport plus faible entre acide linoléique et acide linolénique, incite à utiliser des aliments riches en acides gras polyinsaturés (et en particulier en acide alpha-linolénique C18:3 n-3) pour corriger des rations insuffisamment riches en cet AGPI. Parmi les aliments des vaches laitières, le lin est un aliment particulièrement riche en C18:3 n-3. La diminution des rejets de gaz à effet de serre (dont le méthane) est également une préoccupation actuelle des filières animales. De nombreux essais de supplémentation en lin, sous différentes formes, ont été publiés ces dernières années, et les données disponibles permettent de tirer des conclusions sur ces effets attendus. Le présent article fait le point sur les disponibilités en lin et sur les différentes formes d’apport dans les rations. Les effets du lin sur la production laitière, sur la composition du lait et des produits laitiers, la production de méthane et la reproduction sont passés en revue. L’analyse des effets sur le lait s’appuie sur 41 essais zootechniques publiés. La culture de lin oléagineux est peu importante en France. L’approvisionnement est fait dans des pays européens et au Canada. Les variétés présentent des teneurs variables en acide alpha-linolénique. L’introduction de lin dans la ration diminue un peu la quantité de MS ingérée mais ne modifie en général pas la production laitière (volumes et taux). La teneur du lait en AG saturés diminue et le pourcentage en C18:1-trans est augmenté, et ce d’autant plus que l’apport de lipides se fait sous forme non protégée (graines extrudées, huile) et avec des rations riches en amidon (pour les AG trans). La teneur en C18:2 n-6 n’est en moyenne pas modifiée, sauf par l’apport d’huile. La proportion en C18:3 n-3 du lait est multipliée en moyenne par 2 ou 3 pour les formes pratiques les plus efficaces (graines aplaties, farine), et peut atteindre jusqu’à 1,4% des AG du lait avec ce type de supplémentations. Il n’apparaît pas d’effet dose de lipides apportée pour le C18:2 n-6 et le C18:3 n-3, alors qu’il en existe un pour les C18:1-trans. Le beurre et les fromages ont la même composition en AG que le lait dont ils proviennent. Les qualités organoleptiques de beurres et fromages ne sont pas modifiées par l’addition de lin dans la ration. Différents effets sont cités dans la bibliographie pour expliquer une augmentation potentielle de la fertilité, qui reste à confirmer. La production ruminale de méthane est diminuée par l’ajout de lin dans la ration. En conclusion, l’ajout de lin à la ration des vaches laitières a des effets analogues à ceux d’introduction d’herbe dans le régime fourrager, à l’exception d’une teneur en AG trans supérieure.

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Kore, Sudarshan D. „Sustainable Production of Concrete using Coir Fibres“. IOP Conference Series: Earth and Environmental Science 795, Nr. 1 (01.06.2021): 012006. http://dx.doi.org/10.1088/1755-1315/795/1/012006.

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Henry, James J., James Goldbach, Sean Stabler, Samuel Devisme und Jerome Chauveau. „Advancements in the production of meltblown fibres“. Filtration + Separation 53, Nr. 3 (Mai 2016): 36–40. http://dx.doi.org/10.1016/s0015-1882(16)30123-9.

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Maurice, Terrence, und Jennifer Grealy. „Process for the production of protein fibres“. Nutrition Research 5, Nr. 9 (September 1985): i. http://dx.doi.org/10.1016/s0271-5317(85)80129-9.

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Foroughi, Javad, Geoffrey M. Spinks, Gordon G. Wallace und Philip G. Whitten. „Production of polypyrrole fibres by wet spinning“. Synthetic Metals 158, Nr. 3-4 (Februar 2008): 104–7. http://dx.doi.org/10.1016/j.synthmet.2007.12.008.

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Azarova, M. T., und M. E. Kazakov. „World production and consumption of carbon fibres“. Fibre Chemistry 42, Nr. 5 (März 2011): 271–77. http://dx.doi.org/10.1007/s10692-011-9269-4.

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Ramdhonee, Amit, und Pratima Jeetah. „Production of wrapping paper from banana fibres“. Journal of Environmental Chemical Engineering 5, Nr. 5 (Oktober 2017): 4298–306. http://dx.doi.org/10.1016/j.jece.2017.08.011.

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Bhatt, Pooja, und Alka Goe. „Carbon Fibres: Production, Properties and Potential Use“. Material Science Research India 14, Nr. 1 (09.06.2017): 52–57. http://dx.doi.org/10.13005/msri/140109.

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Carbon fiber is composed of carbon atoms bonded together to form a long chain. The fibers are extremely stiff, strong, and light, and are used in many processes to create excellent building materials. Carbon fiber material comes in a variety of "raw" building-blocks, including yarns, uni-directional, weaves, braids, and several others, which are in turn used to create composite parts. The properties of a carbon fiber part are close to that of steel and the weight is close to that of plastic. Thus the strength to weight ratio (as well as stiffness to weight ratio) of a carbon fiber part is much higher than either steel or plastic. Carbon fiber is extremely strong. It is typical in engineering to measure the benefit of a material in terms of strength to weight ratio and stiffness to weight ratio, particularly in structural design, where added weight may translate into increased lifecycle costs or unsatisfactory performance.

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Nakamura, Koji, Taku Kouro, Paul W. Kincade, Alexander Malykhin, Kazuhiko Maeda und K. Mark Coggeshall. „Src Homology 2–containing 5-Inositol Phosphatase (SHIP) Suppresses an Early Stage of Lymphoid Cell Development through Elevated Interleukin-6 Production by Myeloid Cells in Bone Marrow“. Journal of Experimental Medicine 199, Nr. 2 (12.01.2004): 243–54. http://dx.doi.org/10.1084/jem.20031193.

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The Src homology (SH)2–containing inositol 5-phosphatase (SHIP) negatively regulates a variety of immune responses through inhibitory immune receptors. In SHIP−/− animals, we found that the number of early lymphoid progenitors in the bone marrow was significantly reduced and accompanied by expansion of myeloid cells. We exploited an in vitro system using hematopoietic progenitors that reproduced the in vivo phenotype of SHIP−/− mice. Lineage-negative marrow (Lin−) cells isolated from wild-type mice failed to differentiate into B cells when cocultured with those of SHIP−/− mice. Furthermore, culture supernatants of SHIP−/− Lin− cells suppressed the B lineage expansion of wild-type lineage-negative cells, suggesting the presence of a suppressive cytokine. SHIP−/− Lin− cells contained more IL-6 transcripts than wild-type Lin− cells, and neutralizing anti–IL-6 antibody rescued the B lineage expansion suppressed by the supernatants of SHIP−/− Lin− cells. Finally, we found that addition of recombinant IL-6 to cultures of wild-type Lin− bone marrow cells reproduced the phenotype of SHIP−/− bone marrow cultures: suppression of B cell development and expansion of myeloid cells. The results identify IL-6 as an important regulatory cytokine that can suppress B lineage differentiation and drive excessive myeloid development in bone marrow.

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LEFAUCHEUR, L. „Les différents types de fibres musculaires chez le porc. Conséquences sur la production de viande“. INRAE Productions Animales 2, Nr. 3 (10.07.1989): 205–13. http://dx.doi.org/10.20870/productions-animales.1989.2.3.4414.

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Le muscle squelettique est essentiellement composé de fibres musculaires généralement classées en fonction de leur vitesse de contraction et/ou de leur métabolisme énergétique. Les fibres musculaires se différencient essentiellement pendant les périodes embryonnaire, foetale et post-natale précoce. Un pourcentage élevé de fibres à contractions lentes et de fibres de faible diamètre semble favorable pour les qualités technologiques et organoleptiques de la viande. Au plan des performances de croissance, le nombre total de fibres musculaires, critère définitivement fixé avant la naissance, et la quantité d’ADN sont les principaux facteurs déterminant le potentiel de croissance musculaire. La croissance en diamètre n’est importante à considérer qu’après avoir tenu compte du nombre total de fibres. En conclusion, la recherche d’un grand nombre de fibres musculaires et du maximum de fibres à contractions lentes semble pouvoir répondre aux impératifs d’une production en quantité d’une viande de qualité.

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Shi, Bin Bin. „Application Research of Automatic Guided Vehicle System Based on LIN Bus“. Advanced Materials Research 267 (Juni 2011): 710–14. http://dx.doi.org/10.4028/www.scientific.net/amr.267.710.

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The application model of automatic guided vehicle system based on LIN bus with a chip of MC9S12XEP100 is provided.The hardware circuit and software design process are given.The description about design of LIN bus interface are given in detail. The causes of interference problems in the IC production line are also analyzed,and the solving paths offered.

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Abete-Luzi, Patricia, Tetsunari Fukushige, Sijung Yun, Michael W. Krause und David M. Eisenmann. „New Roles for the Heterochronic Transcription Factor LIN-29 in Cuticle Maintenance and Lipid Metabolism at the Larval-to-Adult Transition in Caenorhabditis elegans“. Genetics 214, Nr. 3 (23.01.2020): 669–90. http://dx.doi.org/10.1534/genetics.119.302860.

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Temporal regulation of gene expression is a crucial aspect of metazoan development. In the roundworm Caenorhabditis elegans, the heterochronic pathway controls multiple developmental events in a time-specific manner. The most downstream effector of this pathway, the zinc-finger transcription factor LIN-29, acts in the last larval stage (L4) to regulate elements of the larval-to-adult switch. Here, we explore new LIN-29 targets and their implications for this developmental transition. We used RNA-sequencing to identify genes differentially expressed between animals misexpressing LIN-29 at an early time point and control animals. Among 230 LIN-29-activated genes, we found that genes encoding cuticle collagens were overrepresented. Interestingly, expression of lin-29 and some of these collagens was increased in adults with cuticle damage, suggesting a previously unknown function for LIN-29 in adult cuticle maintenance. On the other hand, genes involved in fat metabolism were enriched among 350 LIN-29-downregulated targets. We used mass spectrometry to assay lipid content in animals overexpressing LIN-29 and observed reduced fatty acid levels. Many LIN-29-repressed genes are normally expressed in the intestine, suggesting cell-nonautonomous regulation. We identified several LIN-29 upregulated genes encoding signaling molecules that may act as mediators in the regulation of intestinally expressed genes encoding fat metabolic enzymes and vitellogenins. Overall, our results support the model of LIN-29 as a major regulator of adult cuticle synthesis and integrity, and as the trigger for metabolic changes that take place at the important transition from rapid growth during larval life to slower growth and offspring production during adulthood.

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Baričević, Ana, Katarina Didulica, Marina Frančić Smrkić und Marija Jelčić Rukavina. „Cementitious Composites Reinforced with Waste Fibres from the Production of High-Quality Construction Textiles“. Materials 15, Nr. 4 (21.02.2022): 1611. http://dx.doi.org/10.3390/ma15041611.

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In general, 20–25% of the original fibre weight is considered waste in the production of high-quality textiles for the construction sector. A market analysis has shown that in the Republic of Croatia alone, up to 327 tonnes of this waste is produced annually, which is enough to reinforce 50 to 150 thousand m3 of cementitious composites. This preliminary study aims to evaluate the contribution of glass, basalt and carbon fibres generated as waste in the local production of high-performance technical textiles, to the fresh and hardened properties of fibre reinforced mortars. In order to investigate the influence of fibres, three types of fibres in two different lengths (5 and 10 mm) were used, while the amount of fibres was constant. The obtained results show that due to the fibre presence, workability is reduced regardless of the type and length of the fibre. The tested fibres have a negligible effect on compressive strength, but the use of basalt and carbon fibres increases the tensile strength. Furthermore, all three types have positive influence on the toughness and volumetric deformations, although to a greater extent in the use of 10 mm long fibres and carbon fibres.

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Wrischnik, L. A., und C. J. Kenyon. „The role of lin-22, a hairy/enhancer of split homolog, in patterning the peripheral nervous system of C. elegans“. Development 124, Nr. 15 (01.08.1997): 2875–88. http://dx.doi.org/10.1242/dev.124.15.2875.

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In C. elegans, six lateral epidermal stem cells, the seam cells V1-V6, are located in a row along the anterior-posterior (A/P) body axis. Anterior seam cells (V1-V4) undergo a fairly simple sequence of stem cell divisions and generate only epidermal cells. Posterior seam cells (V5 and V6) undergo a more complicated sequence of cell divisions that include additional rounds of stem cell proliferation and the production of neural as well as epidermal cells. In the wild type, activity of the gene lin-22 allows V1-V4 to generate their normal epidermal lineages rather than V5-like lineages. lin-22 activity is also required to prevent additional neurons from being produced by one branch of the V5 lineage. We find that the lin-22 gene exhibits homology to the Drosophila gene hairy, and that lin-22 activity represses neural development within the V5 lineage by blocking expression of the posterior-specific Hox gene mab-5 in specific cells. In addition, in order to prevent anterior V cells from generating V5-like lineages, wild-type lin-22 gene activity must inhibit (directly or indirectly) at least five downstream regulatory gene activities. In anterior body regions, lin-22(+) inhibits expression of the Hox gene mab-5. It also inhibits the activity of the achaete-scute homolog lin-32 and an unidentified gene that we postulate regulates stem cell division. Each of these three genes is required for the expression of a different piece of the ectopic V5-like lineages generated in lin-22 mutants. In addition, lin-22 activity prevents two other Hox genes, lin-39 and egl-5, from acquiring new activities within their normal domains of function along the A/P body axis. Some, but not all, of the patterning activities of lin-22 in C. elegans resemble those of hairy in Drosophila.

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Shibuya, A., K. Nagayoshi, K. Nakamura und H. Nakauchi. „Lymphokine requirement for the generation of natural killer cells from CD34+ hematopoietic progenitor cells“. Blood 85, Nr. 12 (15.06.1995): 3538–46. http://dx.doi.org/10.1182/blood.v85.12.3538.bloodjournal85123538.

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We have established a cell culture system without stromal cells that allows the CD34+ hematopoietic progenitor cells (HPC) to differentiate into natural killer (NK) cells. CD34+Lin (CD3, CD16, CD56)-cells were purified using fluorescence-activated cell sorting from normal adult bone marrow (BM) and cultured for 28 days in medium supplemented with interleukin-2 (IL-2) and stem cell factor (SCF). NK (CD3-CD16-CD56+) cells were generated in a dose-dependent manner in response to SCF. NK cells originated from CD34+CD33+Lin- cells, but they were barely detectable in cultures of CD34+CD33-Lin- cells. However, on addition of IL-3, an induced differentiation of NK cells from CD34+CD33-Lin- cells was observed, although at a lower frequency. Supplementing of the cell cultures with SCF alone or both SCF and IL-3 for the first 7 days followed by IL-2 for the next 21 days is essential for production of NK cells from CD34+CD33+Lin- cells and from CD34+CD33-Lin- cells, respectively. These data provide direct evidence that NK cells arise from CD34+HPC and show the minimum lymphokine requirement for their differentiation.

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Shahriarinour, Mahdi, Mohd Noor Abdul Wahab, Shuhaimi Mustafa, Rosfarizan Mohamad und Arbakariya B. Ariff. „Effect of various pretreatments of oil palm empty fruit bunch fibres for subsequent use as substrate on the performance of cellulase production by Aspergillus terreus“. BioResources 6, Nr. 1 (07.12.2010): 291–307. http://dx.doi.org/10.15376/biores.6.1.291-307.

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The possibility of using treated oil palm empty fruit bunch (OPEFB) fibres as substrate for cellulase production by Aspergillus terreus was studied using shaking flask fermentation. The effect of different chemical pretreatments, i.e. formic acid, acetic acid, propylamine, phosphoric acid, and n-butylamine, on the suitability of OPEFB fibres as fermentation substrate was investigated. The findings revealed that pretreatment with these chemicals significantly (P<0.05) increased the cellulose and reduced the lignin contents prior to enzymatic hydrolysis. However, fermentation using OPEFB fibres pretreated with phosphoric acid gave the highest cellulase production, which was related to high cellulose content. Further improvement in cellulase production was obtained when the chemically pretreated OPEFB fibres were subsequently treated hydrothermally (autoclaved at 160oC for 10 min) and then biologically (using effective microorganisms). The final activity of the three main components of cellulase (FPase, CMCase, and β-glucosidase) obtained in fermentation by A. terreus using optimally treated OPEFB fibres was (0.77 U mL−1, 8.5 U mL-1, and 6.1 U mL-1), respectively. The production of all these three major components of cellulase using pretreated OPEFB fibres (i.e. chemical, hydrothermal, and biological) were about three times higher than those obtained from fermentation using untreated OPEFB fibres.

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Hajji, Rabiaa, Achraf Ghorbel und EtAmmarBen Brahim. „CRYOGELS POREUX A BASE DES FIBRES DE LIN TRAITEES PAR UN MELANGE LIQUIDE IONIQUE EMIMAC / DMSO.“ International Journal of Advanced Research 5, Nr. 3 (31.03.2017): 1111–18. http://dx.doi.org/10.21474/ijar01/3613.

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Ouagne, Pierre, Laurent Bizet, Christophe Bailey und Joël Bréard. „Analyse des couplages hydromécaniques sur renfort à base de fibres de lin. Application aux procédés d'infusion“. Revue des composites et des matériaux avancés 18, Nr. 2 (31.08.2008): 191–96. http://dx.doi.org/10.3166/rcma.18.191-196.

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Roussière, Fabrice, Laurent Vrévin, Dominique Burr und Christophe Baley. „Etude du comportement mécanique en traction de composites polyester/mats de fibres végétales (lin et chanvre)“. Revue des composites et des matériaux avancés 18, Nr. 2 (31.08.2008): 209–14. http://dx.doi.org/10.3166/rcma.18.209-214.

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Roland, Jean-Claude, Michéle Mosiniak und Denis Roland. „Dynamique du positionnement de la cellulose dans les parois des fibres textiles du lin (Linum usitatissimum)“. Acta Botanica Gallica 142, Nr. 5 (Januar 1995): 463–84. http://dx.doi.org/10.1080/12538078.1995.10515271.

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YILDIRIM, Fatma Filiz, Osman Ozan AVİNÇ und Arzu YAVAŞ. „Soybean Protein Fibres Part 1: Structure, Production and Environmental Effects of Soybean Protein Fibres“. Uludağ University Journal of The Faculty of Engineering 19, Nr. 2 (21.12.2014): 29. http://dx.doi.org/10.17482/uujfe.91482.

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Bodnárová, Lenka, Petra Macháňová, Daniel Kopkáně, Petr Herka und Pavel Stahel. „Application of Polypropylene Fibre with Various Surface Treatments in Concrete“. Advanced Materials Research 1054 (Oktober 2014): 75–79. http://dx.doi.org/10.4028/www.scientific.net/amr.1054.75.

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The paper presents results of experimental works focused on property verification of concretes with an admixture of polypropylene fibres with various surface treatments. Common production polypropylene fibres with surface lubrication, polypropylene fibres without surface treatment and low temperature plasma treated polypropylene fibres were used for the testing.

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Ramsfjell, V., OJ Borge, OP Veiby, J. Cardier, MJ Jr Murphy, SD Lyman, S. Lok und SE Jacobsen. „Thrombopoietin, but not erythropoietin, directly stimulates multilineage growth of primitive murine bone marrow progenitor cells in synergy with early acting cytokines: distinct interactions with the ligands for c-kit and FLT3“. Blood 88, Nr. 12 (15.12.1996): 4481–92. http://dx.doi.org/10.1182/blood.v88.12.4481.bloodjournal88124481.

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Thrombopoietin (Tpo), the ligand for c-mpl, has been shown to be the principal regulator of megakaryocytopoiesis and platelet production. The ability of Tpo to potently stimulate the growth of committed megakaryocyte (Mk) progenitor cells has been studied in detail. Murine fetal liver cells, highly enriched in primitive progenitors, have been shown to express c-mpl, but little is known about the ability of Tpo to stimulate the growth and differentiation of primitive multipotent bone marrow (BM) progenitor cells. Here, we show that Tpo alone and in combination with early acting cytokines can stimulate the growth and multilineage differentiation of Lin-Sca-1+ BM progenitor cells. In particular, Tpo potently synergized with the ligands for c-kit (stem cell factor [SCF]) and flt3 (FL) to stimulate an increase in the number and size of clones formed from Lin-Sca-1+ progenitors. When cells were plated at 1 cell per well, the synergistic effect of Tpo was observed both in fetal calf serum-supplemented and serum-depleted medium and was decreased if the addition of Tpo to cultures was delayed for as little as 24 hours, suggesting that Tpo is acting directly on the primitive progenitors. Tpo added to SCF + erythropoietin (Epo)-supplemented methylcellulose cultures potently enhanced the formation of multilineage colonies containing granulocytes, macrophages, erythrocytes, and Mks. SCF potently enhanced Tpo-stimulated production of high-ploidy Mks from Lin- Sca-1+ progenitors, whereas the increased growth response obtained when combining Tpo with FL did not translate into increased Mk production. The ability of Tpo and SCF to synergistically enhance the growth of Lin- Sca-1+ progenitors was predominantly observed in the more primitive rhodamine 123(lo) fraction. Tpo also enhanced growth of Lin- Sca-1+ progenitors when combined with interleukin-3 (IL-3) and IL-11 but not with IL-12, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, or Epo. Epo, which has high homology to Tpo, was unable to stimulate the growth of Lin-Sca-1+ progenitors alone or in combination with SCF or FL, suggesting that c-mpl is expressed on more primitive stages of progenitors than the Epo receptor. Thus, the present studies show the potent ability of Tpo to enhance the growth of primitive multipotent murine BM progenitors in combination with multiple early acting cytokines and documents its unique ability to synergize with SCF to enhance Mk production from such progenitors.

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50

Hess, David A., Phillip E. Herrbrich, Louisa Wirthlin, Timothy P. Craft und Jan A. Nolta. „Isolation of Human CD34- Cells with High Aldehyde Dehydrogenase Activity Reveals a Novel Population with Hematopoietic Repopulating Potential.“ Blood 104, Nr. 11 (16.11.2004): 3214. http://dx.doi.org/10.1182/blood.v104.11.3214.3214.

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Abstract The successful development of stem cell-based therapies requires a thorough understanding of human hematopoietic stem cell (HSC) populations. Human CD34− cells engraft NOD/SCID mice with low efficiency by intravenous (IV) transplant. However, intra-femoral injection into immune deficient mice has identified potent human repopulating cells from CD34+ and CD34− subfractions. We recently described a novel strategy to purify reconstituting HSC from human umbilical cord blood (UCB) by lineage depletion (Lin−) followed by selection of cells with high aldehyde dehydrogenase (ALDH) activity. Hematopoietic progenitor function and in vivo reconstituting ability were exclusively maintained within the ALDHhiLin− population, which demonstrated variable expression of CD34. Here, we compared the repopulating ability of purified CD34+ALDHhiLin− and CD34−ALDHhiLin− populations to traditionally isolated CD34+Lin− and CD34−Lin− cells. Sorting of Lin− cells from human UCB isolated CD34−ALDHhi and CD34+ALDHhi cells (>96% purity) at an overall frequency of 4.4±1.3% or 29.1±3.5%, respectively. In contrast to CD34−Lin− cells, ALDHhiCD34−Lin− cells demonstrated robust clonogenic progenitor function in vitro (1 CFU in 9 cells, n=3), and total colony production was further increased in ALDHhiCD34+Lin− cells (1 CFU in 4.5 cells, n=4) (p<0.05). Human hematopoietic repopulation was consistently observed in the bone marrow (17.2±4.2%), spleen (0.8±0.2%), and peripheral blood (0.7±0.3%) of NOD/SCID β2M null mice 6–8 weeks after IV transplant with 103–104 purified ALDHhiCD34+Lin− cells (n=14). Similarly, intra-femoral injection (IF) of ALDHhiCD34+Lin− cells resulted in robust human repopulation (n=5). IV injection of equivalent doses of either ALDHhiCD34+Lin− or CD34+Lin− cells showed similar levels and frequencies of human hematopoietic engraftment. Repopulating ALDHhiCD34+Lin− cells also differentiated into cells expressing markers for mature myeloid (CD33, CD14), B-lymphoid (CD19, CD20) cells and primitive repopulating cells (CD34+CD38−) at similar frequencies as CD34+Lin− cells (n=5). IV injection of 2x104–1x105 ALDHhiCD34−Lin− cells engrafted at 0.2–0.3% in the BM of 3 of 4 NOD/SCID β2M null mice, whereas IV injection of up to 4x105 CD34−Lin− cells produced no detectable human engraftment (n=6). IF-injected ALDHhiCD34−Lin− cells engrafted the injected bone in 2 of 3 NOD/SCID mice at low levels and did not efficiently migrate to the non-injected femur or tibiae. In summary, the human UCB ALDHhiLin− population includes both CD34+ and CD34− cells capable of bone marrow homing and hematopoietic reconstitution. Therefore, isolation of CD34− cells based on high ALDH activity may reveal a novel population of hematopoietic stem and progenitor cells.

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