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1
Fonseca, Roberto, Gustavo Santos und Paulo Mourão. „Effects of polysaccharides enriched in 2,4-disulfated fucose units on coagulation, thrombosis and bleeding“. Thrombosis and Haemostasis 102, Nr. 11 (2009): 829–36. http://dx.doi.org/10.1160/th08-11-0773.
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SummarySulfated polysaccharides from marine invertebrates have welldefined structures and constitute a reliable class of molecules for structure-activity relationship studies.We tested the effects of two of these polysaccharides,namely a sulfated fucan and a fucosylated chondroitin sulfate, on coagulation, thrombosis and bleeding. The compounds share similar 2,4-disulfated fucose units, which are required for high anticoagulant activity in this class of polymer.These residues occur either as branches in fucosylated chondroitin sulfate or as components of the linear chain in the sulfated fucan.These polysaccharides possess anticoagulant activity but differ significantly in their mechanisms of action.The fucosylated chondroitin sulfate inhibits thrombin by heparin cofactor II, whereas sulfated fucan inhibits thrombin by both antithrombin and heparin cofactor II. In addition, these polysaccharides also have serpin-independent anticoagulant activities. Fucosylated chondroitin sulfate, but not sulfated fucan, activates factor XII. As a result of the complex anticoagulant mechanism, the invertebrate polysaccharides differ in their effects on experimental thrombosis. For instance, the sulfated fucan inhibits venous thrombosis at lower doses than fucosylated chondroitin sulfate. In contrast, fucosylated chondroitin sulfate is significantly more potent than sulfated fucan in arterial thrombosis. Finally, fucosylated chondroitin sulfate increases bleeding, while sulfated fucan has only a discrete effect. In conclusion, the location of 2,4-disulfated fucose units in the polysaccharide chains dictates the effects on coagulation, thrombosis and bleeding.
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Vessella, Giulia, José Antonio Vázquez, Jesús Valcárcel, Laura Lagartera, Dianélis T. Monterrey, Agatha Bastida, Eduardo García-Junceda, Emiliano Bedini, Alfonso Fernández-Mayoralas und Julia Revuelta. „Deciphering Structural Determinants in Chondroitin Sulfate Binding to FGF-2: Paving the Way to Enhanced Predictability of Their Biological Functions“. Polymers 13, Nr. 2 (19.01.2021): 313. http://dx.doi.org/10.3390/polym13020313.
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Controlling chondroitin sulfates (CSs) biological functions to exploit their interesting potential biomedical applications requires a comprehensive understanding of how the specific sulfate distribution along the polysaccharide backbone can impact in their biological activities, a still challenging issue. To this aim, herein, we have applied an “holistic approach” recently developed by us to look globally how a specific sulfate distribution within CS disaccharide epitopes can direct the binding of these polysaccharides to growth factors. To do this, we have analyzed several polysaccharides of marine origin and semi-synthetic polysaccharides, the latter to isolate the structure-activity relationships of their rare, and even unnatural, sulfated disaccharide epitopes. SPR studies revealed that all the tested polysaccharides bind to FGF-2 (with exception of CS-8, CS-12 and CS-13) according to a model in which the CSs first form a weak complex with the protein, which is followed by maturation to tight binding with kD ranging affinities from ~1.31 μM to 130 μM for the first step and from ~3.88 μM to 1.8 nM for the second one. These binding capacities are, interestingly, related with the surface charge of the 3D-structure that is modulated by the particular sulfate distribution within the disaccharide repeating-units.
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Silchenko, Artem S., Anton B. Rasin, Anastasiya O. Zueva, Mikhail I. Kusaykin, Tatiana N. Zvyagintseva, Anatoly I. Kalinovsky, Valeriya V. Kurilenko und Svetlana P. Ermakova. „Fucoidan Sulfatases from Marine Bacterium Wenyingzhuangia fucanilytica CZ1127T“. Biomolecules 8, Nr. 4 (21.09.2018): 98. http://dx.doi.org/10.3390/biom8040098.
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Fucoidans belong to a structurally heterogeneous class of sulfated polysaccharides isolated from brown algae. They have a wide spectrum of biological activities. The complex structures of these polysaccharides hinder structure-activity relationships determination. Fucoidan sulfatases can make useful tools for the determination of the fine chemical structure of fucoidans. In this study, identification and preparation of two recombinant sulfatases able to catalyze the cleavage of sulfate groups from fragments of fucoidan molecules is described for the first time. Two genes of sulfatases swf1 and swf4 of the marine bacterium Wenyingzhuangia fucanilytica CZ1127T were cloned and the proteins were produced in Escherichia coli cells. Sulfatases SWF1 and SWF4 are assigned to S1_17 and S1_25 subfamilies of formylglycine-dependent enzymes of S1 family (SulfAtlas). Some molecular and biochemical characteristics of recombinant fucoidan sulfatases have been studied. Detailed specificity and catalytic features of sulfatases were determined using various sulfated fucooligosaccharides. Structures of products produced by SWF1 and SWF4 were established by nuclear magnetic resonance (NMR) spectroscopy. Based on the obtained data, the enzymes are classified as fucoidan exo-2O-sulfatase (SWF1) and fucoidan exo-3O-sulfatase (SWF4). In addition, we demonstrated the sequential action of sulfatases on 2,3-di-O-sulfated fucooligosacchrides, which indicates an exolitic degradation pathway of fucoidan by a marine bacterium W. fucanilytica CZ1127T.
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Miranda-Arizmendi, Valeria, Diana Fimbres-Olivarria, Anselmo Miranda-Baeza, Agustín Rascón-Chu, Jorge Marquez-Escalante, Jaime Lizardi-Mendoza, Mayra A. Méndez-Encinas und Elizabeth Carvajal-Millan. „Sulfated polysaccharides from marine diatoms: Insight into molecular characteristics and biological activity“. AIMS Bioengineering 11, Nr. 1 (2024): 110–29. http://dx.doi.org/10.3934/bioeng.2024007.
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<abstract> <p>Marine algae are a valuable source of sulfated polysaccharides presenting varied structural characteristics and biological activities. Regarding sulfated polysaccharides extracted from marine microalgae, molecular characteristics and bioactivity have yet to be fully explored, especially in diatoms. Sulfated polysaccharides from marine diatoms have great potential to produce numerous health benefits and lead to new biomedical materials. Nevertheless, these potential applications are based on the polysaccharide molecular characteristics, which define their functional properties. Therefore, a detailed understanding of sulfated polysaccharides from marine diatoms may represent the starting point for a broad development of innovative applications, especially in the biomedical area. In this context, the present manuscript aims to review marine diatom sulfated polysaccharides' molecular characteristics and biological activity, looking for a more profound knowledge of these macromolecules and their potential applications.</p> </abstract>
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Jun, Joon-Young, Min-Jeong Jung, In-Hak Jeong, Koji Yamazaki, Yuji Kawai und Byoung-Mok Kim. „Antimicrobial and Antibiofilm Activities of Sulfated Polysaccharides from Marine Algae against Dental Plaque Bacteria“. Marine Drugs 16, Nr. 9 (27.08.2018): 301. http://dx.doi.org/10.3390/md16090301.
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Dental plaque biofilms cause various dental diseases; therefore, inhibiting the growths of the dental plaque bacteria which produce biofilms can be a strategy for preventing dental disease. Certain sulfated polysaccharides from marine algae exert antimicrobial activities against human bacterial pathogens in addition to their physiological benefits. On the basis of these observations, the antimicrobial and antibiofilm activities of sulfated polysaccharides from different marine algae were evaluated against dental plaque bacteria. Among the sulfated polysaccharides, a fucoidan from Fucus vesiculosus showed notable antimicrobial activities against the selected dental plaque bacteria, including some foodborne pathogenic bacteria. The minimum inhibitory concentrations were of 125 to 1000 µg mL−1. Regarding the antibiofilm activity, the fucoidan at the concentrations of above 250 µg mL−1 completely suppressed the biofilm formations and planktonic cell growths of Streptococcus mutans and S. sobrinus. However, no eliminative effect on the completed biofilm was observed. The fucoidan consisted of almost fucose base polysaccharide containing approximately 14.0% sulfate content. The average molecular weight of the fucoidan was changed by heat treatment (121 °C for 15 min) and it affected the antimicrobial activity.
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He, Meijia, Yajing Yang, Zhuling Shao, Junyan Zhang, Changning Feng, Lei Wang und Wenjun Mao. „Chemical Structure and Anticoagulant Property of a Novel Sulfated Polysaccharide from the Green Alga Cladophora oligoclada“. Marine Drugs 19, Nr. 10 (29.09.2021): 554. http://dx.doi.org/10.3390/md19100554.
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Marine macroalgae are efficient producers of sulfated polysaccharides. The algal sulfated polysaccharides possess diverse bioactivities and peculiar chemical structures, and represent a great potential source to be explored. In the present study, a heparinoid-active sulfated polysaccharide was isolated from the green alga Cladophora oligoclada. Results of chemical and spectroscopic analyses indicated that the sulfated polysaccharide was composed of →6)-β-d-Galp-(1→, β-d-Galp-(1→, →6)-α-d-Glcp-(1→ and →3)-β-d-Galp-(1→ units with sulfate esters at C-2/C-4 of →6)-β-d-Galp-(1→, C-6 of →3)-β-d-Galp-(1→ and C-3 of →6)-α-d-Glcp-(1→ units. The branches consisting of β-d-Galp-(1→ and →6)-β-d-Galp-(1→ units were located in C-3 of →6)-β-d-Galp-(1→ units. The sulfated polysaccharide exhibited potent anticoagulant activity in vitro and in vivo as evaluated by activated partial thromboplastin time (APTT), thrombin time, and the fibrinogen level. For the APTT, the signal for clotting time was more than 200 s at 100 μg/mL in vitro and at 15 mg/kg in vivo. The obvious thrombolytic activity of the sulfated polysaccharide in vitro was also found. The mechanism analysis of anticoagulant action demonstrated that the sulfated polysaccharide significantly inhibited the activities of all intrinsic coagulation factors, which were less than 1.0% at 50 μg/mL, but selectively inhibited common coagulation factors. Furthermore, the sulfated polysaccharide strongly stimulated the inhibition of thrombin by potentiating antithrombin-III (AT-III) or heparin cofactor-II, and it also largely promoted the inhibition of factor Xa mediated by AT-III. These results revealed that the sulfated polysaccharide from C. oligoclada had potential to become an anticoagulant agent for prevention and therapy of thrombotic diseases.
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Carvalhal, Francisca, Ricardo Cristelo, Diana Resende, Madalena Pinto, Emília Sousa und Marta Correia-da-Silva. „Antithrombotics from the Sea: Polysaccharides and Beyond“. Marine Drugs 17, Nr. 3 (16.03.2019): 170. http://dx.doi.org/10.3390/md17030170.
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Marine organisms exhibit some advantages as a renewable source of potential drugs, far beyond chemotherapics. Particularly, the number of marine natural products with antithrombotic activity has increased in the last few years, and reports show a wide diversity in scaffolds, beyond the polysaccharide framework. While there are several reviews highlighting the anticoagulant and antithrombotic activities of marine-derived sulfated polysaccharides, reports including other molecules are sparse. Therefore, the present paper provides an update of the recent progress in marine-derived sulfated polysaccharides and quotes other scaffolds that are being considered for investigation due to their antithrombotic effect.
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Li, Peipei, Junlu Bai, XiaoJun Zhang, Zhongyong Yan, Pengfei He und Yin Chen. „Structure and Anticoagulant Activity of a Galactofuranose-Containing Sulfated Polysaccharide from the Green Seaweed, Codium isthmocladum“. Molecules 27, Nr. 22 (18.11.2022): 8012. http://dx.doi.org/10.3390/molecules27228012.
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A water-soluble sulfated polysaccharide, F2-1, was obtained from the marine green alga, Codium isthmocladum, using ion-exchange and size-exclusion chromatography. Structure analysis showed that the F2-1 was a sulfated arabinan comprising Ara, Rha, Man, Gal, and Xyl with an 18% sulfate content and a molecular weight of 100 kDa. Methylation analysis combined with desulfation, GC-MS, IR, and NMR spectroscopy showed that the backbone of F2-1 was →4)-β-L-Arap(1→ residue. Its 2-O and/or 3-O positions showed sulfate modification; additionally, the 2-O or 3-O position showed branch points. The side chains were composed of →5)-β-D-Galf, (1→2,6)-β-D-Galf(1→, (1→2)-β-L-Rhap4S, →4)-α-D-Glcp(1→, and terminal α-D-Galp(1→ and β-D-Xylp(1→. Polysaccharides containing β-D-galactofuranose are rarely found in seaweed. F2-1 exhibited significant anticoagulant activity in vitro. Our findings suggested that the green-tide alga, Codium isthmocladum, can be considered as a useful resource for bioactive polysaccharides.
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Vilanova, Eduardo, Priscilla J. Ciodaro, Francisco F. Bezerra, Gustavo R. C. Santos, Juan J. Valle-Delgado, Dario Anselmetti, Xavier Fernàndez-Busquets und Paulo A. S. Mourão. „Adhesion of freshwater sponge cells mediated by carbohydrate–carbohydrate interactions requires low environmental calcium“. Glycobiology 30, Nr. 9 (20.02.2020): 710–21. http://dx.doi.org/10.1093/glycob/cwaa014.
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Abstract Marine ancestors of freshwater sponges had to undergo a series of physiological adaptations to colonize harsh and heterogeneous limnic environments. Besides reduced salinity, river-lake systems also have calcium concentrations far lower than seawater. Cell adhesion in sponges is mediated by calcium-dependent multivalent self-interactions of sulfated polysaccharide components of membrane-bound proteoglycans named aggregation factors. Cells of marine sponges require seawater average calcium concentration (10 mM) to sustain adhesion promoted by aggregation factors. We demonstrate here that the freshwater sponge Spongilla alba can thrive in a calcium-poor aquatic environment and that their cells are able to aggregate and form primmorphs with calcium concentrations 40-fold lower than that required by marine sponges cells. We also find that their gemmules need calcium and other micronutrients to hatch and generate new sponges. The sulfated polysaccharide purified from S. alba has sulfate content and molecular size notably lower than those from marine sponges. Nuclear magnetic resonance analyses indicated that it is composed of a central backbone of non- and 2-sulfated α- and β-glucose units decorated with branches of α-glucose. Assessments with atomic force microscopy/single-molecule force spectroscopy show that S. alba glucan requires 10-fold less calcium than sulfated polysaccharides from marine sponges to self-interact efficiently. Such an ability to retain multicellular morphology with low environmental calcium must have been a crucial evolutionary step for freshwater sponges to successfully colonize inland waters.
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Fimbres-Olivarria, Diana, Jorge Marquez-Escalante, Karla G. Martínez-Robinson, Valeria Miranda-Arizmendi, Yubia De Anda-Flores, Agustín Rascon-Chu, Francisco Brown-Bojorquez und Elizabeth Carvajal-Millan. „Physicochemical and Microstructural Characteristics of Sulfated Polysaccharide from Marine Microalga“. Analytica 4, Nr. 4 (05.12.2023): 527–37. http://dx.doi.org/10.3390/analytica4040036.
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Marine algae are a valuable source of polysaccharides. However, the information available on sulfated polysaccharides from microalgae is limited. Navicula sp. is a microalga present in the Sea of Cortez, of which little is known regarding their polysaccharides’ properties. This study investigated the physicochemical and microstructural characteristics of Navicula sp. sulfated polysaccharide (NSP). The Fourier transform infrared spectrum of NSP showed distinctive bands (1225 and 820 cm−1, assigned to S–O and C–O–S stretching, respectively), confirming the molecular identity. NSP registered molecular weight, intrinsic viscosity, a radius of gyration, and a hydrodynamic radius of 1650 kDa, 197 mL/g, 61 nm, and 36 nm, respectively. The zeta potential, electrophoretic mobility, conductivity, and diffusion coefficient of the molecule were −5.8 mV, −0.45 µm cm/s V, 0.70 mS/cm, and 2.9 × 10−9 cm2/s, respectively. The characteristic ratio and persistence length calculated for NSP were 4.2 and 1.3 nm, suggesting a nonstiff polysaccharide chain conformation. The Mark–Houwink–Sakurada α and K constants were 0.5 and 1.67 × 10−1, respectively, indicating a molecular random coil structure. NSP scanning electron microscopy revealed a rough and porous surface. Knowing these polysaccharides’ physicochemical and microstructural characteristics can be the starting point for elucidating their structure–function relationship as a valuable tool in advanced biomaterial design.
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Zhong, Qiwu, Bin Wei, Sijia Wang, Songze Ke, Jianwei Chen, Huawei Zhang und Hong Wang. „The Antioxidant Activity of Polysaccharides Derived from Marine Organisms: An Overview“. Marine Drugs 17, Nr. 12 (29.11.2019): 674. http://dx.doi.org/10.3390/md17120674.
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Marine-derived antioxidant polysaccharides have aroused extensive attention because of their potential nutritional and therapeutic benefits. However, the comprehensive comparison of identified marine-derived antioxidant polysaccharides is still inaccessible, which would facilitate the discovery of more efficient antioxidants from marine organisms. Thus, this review summarizes the sources, chemical composition, structural characteristics, and antioxidant capacity of marine antioxidant polysaccharides, as well as their protective in vivo effects mediated by antioxidative stress reported in the last few years (2013–2019), and especially highlights the dominant role of marine algae as antioxidant polysaccharide source. In addition, the relationships between the chemical composition and structural characteristics of marine antioxidant polysaccharides with their antioxidant capacity were also discussed. The antioxidant activity was found to be determined by multiple factors, including molecular weight, monosaccharide composition, sulfate position and its degree.
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N, Saranya, Sri Abarajitha R, Anandhasayanan T, Jaivignesh D, Mugunthan B, Yuvaraj Dinakarkumar und Kirubanandan Shanmugam. „Screening of Antiviral Efficacy of Few Seaweeds of Tamil Nadu Coast“. Proceedings of Anticancer Research 7, Nr. 5 (25.09.2023): 17–29. http://dx.doi.org/10.26689/par.v7i5.5161.
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Lately there has been a lot of interest worldwide in studies on the antiviral activities of marine natural secondary metabolites, notably marine polysaccharides. It has been established that polysaccharides made from marine sources and their derivatives have antiviral effects against potent viruses. Agricultural, biological, food, and pharmaceutical industries all make extensive use of goods obtained from algae. The most well-known chemical components found in algae are polysaccharides, which have been the subject of a variety of studies because of their varied bioactivities. Polysaccharides made from algae have recently risen to the top of pharmaceutical research due to their fascinating antiviral potential. Currently, COVID-19 can be prevented with vaccination, but the brown alga Sargassum wightii has several bioactive compounds that have the following qualities and may be a better option. S. wightii is one of the marine algae species that is rich in sulfated polysaccharides, the secondary metabolites which have antiviral action and the capacity to prevent viral proliferation. Fucoidan, a long-chain sulfated polysaccharide found in various brown algae, has potent antiviral effects. Additionally, sulfated polysaccharides from green algae (such as ulvans) and red algae (such as carrageenan), and lectins from red algae (such as griffithsin) have antiviral therapeutic agents against coronaviruses and other viruses. This research focuses on screening seaweeds for possible antiviral compounds to treat viral infections notably COVID-19.
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Cao, Sujian, Yajing Yang, Shan Liu, Zhuling Shao, Xiao Chu und Wenjun Mao. „Immunomodulatory Activity In Vitro and In Vivo of a Sulfated Polysaccharide with Novel Structure from the Green Alga Ulvaconglobata Kjellman“. Marine Drugs 20, Nr. 7 (08.07.2022): 447. http://dx.doi.org/10.3390/md20070447.
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Algae accumulate large amounts of polysaccharides in their cell walls or intercellular regions. Polysaccharides from algae possess high potential as promising candidates for marine drug development. In this study, a sulfated polysaccharide, UCP, from the green alga Ulva conglobata Kjellman was obtained by water extraction, anion-exchange, and size-exclusion chromatography purification, and its structure was characterized by a combination of chemical and spectroscopic methods. UCP mainly consisted of →4)-α/β-l-Rhap-(1→, →4)-β-d-Xylp-(1→ and →4)-β-d-GlcAp-(1→ residues. Sulfate ester groups were substituted mainly at C-3 of →4)-l-Rhap-(1→ and C-2 of →4)-β-d-Xylp-(1→. Partial glycosylation was at C-2 of →4)-α-l-Rhap-(1→ residues. UCP possessed a potent immunomodulatory effect in vitro, evaluated by the assays of lymphocyte proliferation and macrophage phagocytosis. The immunomodulatory activity of UCP in vivo was further investigated using immunosuppressive mice induced by cyclophosphamide. The results showed that UCP markedly increased the spleen and thymus indexes and ameliorated the cyclophosphamide-induced damage to the spleen and thymus. UCP could increase the levels of white blood cells, lymphocytes, and platelets, and improve the hematopoietic inhibition caused by cyclophosphamide. Moreover, UCP significantly promoted the secretions of the immunoglobulin (Ig)G, IgE, and IgM. The data demonstrated that UCP is a novel sulfated polysaccharide and may be a promising immunomodulatory agent.
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Fonseca, Roberto J. C., und Paulo A. S. Mourão. „Pharmacological Activities of Sulfated Fucose-Rich Polysaccharides after Oral Administration: Perspectives for the Development of New Carbohydrate-Based Drugs“. Marine Drugs 19, Nr. 8 (27.07.2021): 425. http://dx.doi.org/10.3390/md19080425.
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Marine organisms are a source of active biomolecules with immense therapeutic and nutraceutical potential. Sulfated fucose-rich polysaccharides are present in large quantities in these organisms with important pharmacological effects in several biological systems. These polysaccharides include sulfated fucan (as fucoidan) and fucosylated chondroitin sulfate. The development of these polysaccharides as new drugs involves several important steps, among them, demonstration of the effectiveness of these compounds after oral administration. The oral route is the more practical, comfortable and preferred by patients for long-term treatments. In the past 20 years, reports of various pharmacological effects of these polysaccharides orally administered in several animal experimental models and some trials in humans have sparked the possibility for the development of drugs based on sulfated polysaccharides and/or the use of these marine organisms as functional food. This review focuses on the main pharmacological effects of sulfated fucose-rich polysaccharides, with an emphasis on the antidislipidemic, immunomodulatory, antitumor, hypoglycemic and hemostatic effects.
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Khan, Bilal Muhammad, Li-Xin Zheng, Wajid Khan, Aftab Ali Shah, Yang Liu und Kit-Leong Cheong. „Antioxidant Potential of Physicochemically Characterized Gracilaria blodgettii Sulfated Polysaccharides“. Polymers 13, Nr. 3 (30.01.2021): 442. http://dx.doi.org/10.3390/polym13030442.
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Marine rhodophyte polysaccharides have a wide range of described biological properties with nontoxic characteristics, and show great potential in prebiotics and the functional foods industries. However, there is a virtual lack of Gracilaria blodgettii polysaccharides (GBP) profiling and their bioactivities. This study was designed while keeping in view the lack of physical and chemical characterization of GBP. This polysaccharide was also not previously tested for any bioactivities. A linear random coil conformation was observed for GBP, which was found to be a polysaccharide. A significant sulfate (w/w, 9.16%) and 3,6-anhydrogalactose (AHG, w/w, 17.97%) content was found in GBP. The significant difference in its setting (27.33 °C) and melting (64.33 °C) points makes it resistant to increasing heat. This, in turn, points to its utility in industrial scale processing and in enhancing the shelf-life of products under high temperatures. A radical scavenging activity of 19.80%, 25.42% and 8.80% was noted for GBP (3 mg/mL) in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (ABTS) and hydroxyl radical (HO) scavenging assays, respectively. Therefore, the findings suggest that Gracilaria blodgettii polysaccharides display a good antioxidant potential and may have potential applications in the functional food industry.
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Figueroa, Fabian A., Roberto T. Abdala-Díaz, Claudia Pérez, Virginia Casas-Arrojo, Aleksandra Nesic, Cecilia Tapia, Carla Durán et al. „Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity“. Marine Drugs 20, Nr. 7 (15.07.2022): 458. http://dx.doi.org/10.3390/md20070458.
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Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the ABTS•+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 µg mL−1 concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 µg mL−1. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.
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Liu, Yixiang, Wenqiang Liu, Yanbo Wang, Yu Ma, Ling Huang, Chao Zou, Donghui Li, Min-Jie Cao und Guang-Ming Liu. „Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli“. Marine Drugs 17, Nr. 12 (10.12.2019): 694. http://dx.doi.org/10.3390/md17120694.
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Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics. This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88. Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88. A structure–activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition. In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides. Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion. In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.
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Christensen, Monica Daugbjerg, Leila Allahgholi, Javier A. Linares-Pastén, Ólafur Friðjónsson, Hörður Guðmundsson, Varsha Kale, Roya R. R. Sardari, Guðmundur Ó. Hreggviðsson und Eva Nordberg Karlsson. „Cloning and Characterization of a Novel N-Acetyl-D-galactosamine-4-O-sulfate Sulfatase, SulA1, from a Marine Arthrobacter Strain“. Marine Drugs 22, Nr. 3 (23.02.2024): 104. http://dx.doi.org/10.3390/md22030104.
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Sulfation is gaining increased interest due to the role of sulfate in the bioactivity of many polysaccharides of marine origin. Hence, sulfatases, enzymes that control the degree of sulfation, are being more extensively researched. In this work, a novel sulfatase (SulA1) encoded by the gene sulA1 was characterized. The sulA1-gene is located upstream of a chondroitin lyase encoding gene in the genome of the marine Arthrobacter strain (MAT3885). The sulfatase was produced in Escherichia coli. Based on the primary sequence, the enzyme is classified under sulfatase family 1 and the two catalytic residues typical of the sulfatase 1 family—Cys57 (post-translationally modified to formyl glycine for function) and His190—were conserved. The enzyme showed increased activity, but not improved stability, in the presence of Ca2+, and conserved residues for Ca2+ binding were identified (Asp17, Asp18, Asp277, and Asn278) in a structural model of the enzyme. The temperature and pH activity profiles (screened using p-nitrocatechol sulfate) were narrow, with an activity optimum at 40–50 °C and a pH optimum at pH 5.5. The Tm was significantly higher (67 °C) than the activity optimum. Desulfation activity was not detected on polymeric substrates, but was found on GalNAc4S, which is a sulfated monomer in the repeated disaccharide unit (GlcA–GalNAc4S) of, e.g., chondroitin sulfate A. The position of the sulA1 gene upstream of a chondroitin lyase gene and combined with the activity on GalNAc4S suggests that there is an involvement of the enzyme in the chondroitin-degrading cascade reaction, which specifically removes sulfate from monomeric GalNAc4S from chondroitin sulfate degradation products.
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Li, Tingting, Haiqiong Ma, Hong Li, Hao Tang, Jinwen Huang, Shiying Wei, Qingxia Yuan et al. „Physicochemical Properties and Anticoagulant Activity of Purified Heteropolysaccharides from Laminaria japonica“. Molecules 27, Nr. 9 (08.05.2022): 3027. http://dx.doi.org/10.3390/molecules27093027.
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Laminaria japonica is widely consumed as a key food and medicine. Polysaccharides are one of the most plentiful constituents of this marine plant. In this study, several polysaccharide fractions with different charge numbers were obtained. Their physicochemical properties and anticoagulant activities were determined by chemical and instrumental methods. The chemical analysis showed that Laminaria japonica polysaccharides (LJPs) and the purified fractions LJP0, LJP04, LJP06, and LJP08 mainly consisted of mannose, glucuronic acid, galactose, and fucose in different mole ratios. LJP04 and LJP06 also contained minor amounts of xylose. The polysaccharide fractions eluted by higher concentration of NaCl solutions showed higher contents of uronic acid and sulfate group. Biological activity assays showed that LJPs LJP06 and LJP08 could obviously prolong the activated partial thromboplastin time (APTT), indicating that they had strong anticoagulant activity. Furthermore, we found that LJP06 exerted this activity by inhibiting intrinsic factor Xase with higher selectivity than other fractions, which may have negligible bleeding risk. The sulfate group may play an important role in the anticoagulant activity. In addition, the carboxyl group and surface morphology of these fractions may affect their anticoagulant activities. The results provide information for applications of L. japonica polysaccharides, especially LJP06 as anticoagulants in functional foods and therapeutic agents.
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Yim, Sung-Kun, Kian Kim, Inhee Kim, SangHo Chun, TaeHwan Oh, Jin-Ung Kim, Jungwon Kim et al. „Inhibition of SARS-CoV-2 Virus Entry by the Crude Polysaccharides of Seaweeds and Abalone Viscera In Vitro“. Marine Drugs 19, Nr. 4 (15.04.2021): 219. http://dx.doi.org/10.3390/md19040219.
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Much attention is being devoted to the potential of marine sulfated polysaccharides as antiviral agents in preventing COVID-19. In this study, sulfated fucoidan and crude polysaccharides, extracted from six seaweed species (Undaria pinnatifida sporophyll, Laminaria japonica, Hizikia fusiforme, Sargassum horneri, Codium fragile, Porphyra tenera) and Haliotis discus hannai (abalone viscera), were screened for their inhibitory activity against SARS-CoV-2 virus entry. Most of them showed significant antiviral activities at an IC50 of 12~289 μg/mL against SARS-CoV-2 pseudovirus in HEK293/ACE2, except for P. tenera (IC50 > 1000 μg/mL). The crude polysaccharide of S. horneri showed the strongest antiviral activity, with an IC50 of 12 μg/mL, to prevent COVID-19 entry, and abalone viscera and H. fusiforme could also inhibit SARS-CoV-2 infection with an IC50 of 33 μg/mL and 47 μg/mL, respectively. The common properties of these crude polysaccharides, which have strong antiviral activity, are high molecular weight (>800 kDa), high total carbohydrate (62.7~99.1%), high fucose content (37.3~66.2%), and highly branched polysaccharides. These results indicated that the crude polysaccharides from seaweeds and abalone viscera can effectively inhibit SARS-CoV-2 entry.
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Vessella, Giulia, Serena Traboni, Antonio Laezza, Alfonso Iadonisi und Emiliano Bedini. „(Semi)-Synthetic Fucosylated Chondroitin Sulfate Oligo- and Polysaccharides“. Marine Drugs 18, Nr. 6 (01.06.2020): 293. http://dx.doi.org/10.3390/md18060293.
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Fucosylated chondroitin sulfate (fCS) is a glycosaminoglycan (GAG) polysaccharide with a unique structure, displaying a backbone composed of alternating N-acetyl-d-galactosamine (GalNAc) and d-glucuronic acid (GlcA) units on which l-fucose (Fuc) branches are installed. fCS shows several potential biomedical applications, with the anticoagulant activity standing as the most promising and widely investigated one. Natural fCS polysaccharides extracted from marine organisms (Echinoidea, Holothuroidea) present some advantages over a largely employed antithrombotic drug such as heparin, but some adverse effects as well as a frequently found structural heterogeneity hamper its development as a new drug. To circumvent these drawbacks, several efforts have been made in the last decade to obtain synthetic and semi-synthetic fCS oligosaccharides and low molecular weight polysaccharides. In this Review we have for the first time collected these reports together, dividing them in two topics: (i) total syntheses of fCS oligosaccharides and (ii) semi-synthetic approaches to fCS oligosaccharides and low molecular weight polysaccharides as well as glycoclusters displaying multiple copies of fCS species.
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Essa, Hanaa L., Hania A. Guirguis, Mayyada M. H. El-Sayed, Dalia Rifaat und Mohamed S. Abdelfattah. „Ultrasonically-Extracted Marine Polysaccharides as Potential Green Antioxidant Alternatives“. Proceedings 67, Nr. 1 (09.11.2020): 23. http://dx.doi.org/10.3390/asec2020-07606.
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Marine-extracted sulfated polysaccharides (SPs) have been the subject of myriad research since they are considered an eco-friendly source of biologically active compounds. Meanwhile, food and pharmaceutical industries are urgently producing natural sugar substitutes and antioxidants as alternatives to synthetic ones which are associated with cytotoxicity and safety issues. This study assesses the potential of using marine SPs obtained via the ultrasonic-assisted extraction of different marine species, to utilize them as antioxidant sugar substitutes. The carbohydrate, total phenolic contents and antioxidant activities were measured for SP extracts of the algal species of Ulva lactuca, Jania rubens and the marine plant mangrove Avicennia marina. These SPs were structurally elucidated by Fourier Transform Infrared (FTIR) spectroscopic and high-performance liquid chromatography (HPLC) analyses. The results revealed that SPs’ highest yield percent was obtained from Ulva lactuca, 5.50 ± 0.25%. The SPs of Avicennia marina had the highest carbohydrate content, 44 ± 1% and antioxidant activity, 78.85 ± 0.06 at the 100 μg/mL concentration and 89.50 ± 0.21 at the 250 μg/mL concentration. Meanwhile, the highest phenolic content was exhibited by algal SPs obtained from Jania rubens, 132.60 ± 2.50 mgGa/g. Results also showed that all extracts have potent antioxidant activity, while the highest antioxidant activity belonged to the SPs of Avicennia marina owing possibly to their balanced glucose and galactose contents as measured by HPLC. This work emphasizes the need to consider sulfated polysaccharides from marine sources for their antioxidant activity and to correlate it with their monosaccharide content to determine the effect of reducing sugar concentration on the antioxidant activity.
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Rathnayake, Anuruddhika Udayangani, Racheal Abuine, Yong-Jae Kim und Hee-Guk Byun. „Anti-Alzheimer’s Materials Isolated from Marine Bio-resources: A Review“. Current Alzheimer Research 16, Nr. 10 (20.11.2019): 895–906. http://dx.doi.org/10.2174/1567205016666191024144044.
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The most common type of dementia found in the elderly population is Alzheimer’s disease. The disease not only impacts the patients and their families but also the society therefore, the main focus of researchers is to search new bioactive materials for treating AD. The marine environment is a rich source of functional ingredients and to date, we can find sufficient research relating to anti- Alzheimer’s compounds isolated from marine environment. Therefore, this review focuses on the anti- Alzheimer’s material from marine bio-resources and then expounds on the anti-Alzheimer’s compounds from marine seaweed, marine animal and marine microorganisms. Moreover, because of the complexity of the disease, different hypothesizes have been elaborated and active compounds have been isolated to inhibit different stages of pathophysiological mechanisms. Sulfated polysaccharides, glycoprotein, and enzymatic hydrolysates from marine seaweeds, peptides, dietary omega-3 polyunsaturated fatty acids and skeletal polysaccharide from marine animals and secondary metabolites from marine microorganism are summarized in this review under the anti-Alzheimer’s compounds from the marine.
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Cao, Sujian, Xiaoxi He, Ling Qin, Meijia He, Yajing Yang, Zhichun Liu und Wenjun Mao. „Anticoagulant and Antithrombotic Properties in Vitro and in Vivo of a Novel Sulfated Polysaccharide from Marine Green Alga Monostroma nitidum“. Marine Drugs 17, Nr. 4 (25.04.2019): 247. http://dx.doi.org/10.3390/md17040247.
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Sulfated polysaccharides from marine algae have high potential as promising candidates for marine drug development. In this study, a homogeneous sulfated polysaccharide from the marine green alga Monostroma nitidum, designated MS-1, was isolated using water extraction and anion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that MS-1 mainly consisted of →3)-α-l-Rhap-(1→ and →2)-α-l-Rhap-(1→ residues, with additional branches consisting of 4-linked β-d-xylose, 4-/6-linked d-glucose, terminal β-d-glucuronic acid, and 3-/2-linked α-l-rhamnose. Sulfate ester groups substituted mainly at C-2/C-4 of →3)-α-l-Rhap-(1→ and C-4 of →2)-α-l-Rhap-(1→ residues, slightly at C-2 of terminal β-d-glucuronic residues. MS-1 exhibited strong anticoagulant activity in vitro and in vivo as evaluated by the activated partial thromboplastin time and thrombin time assays, and significantly decreased platelet aggregation. The anticoagulant activity mechanism of MS-1 was mainly attributed to strong potentiation thrombin by heparin cofactor-II, and it also hastened thrombin and coagulation factor Xa inhibitions by potentiating antithrombin-III. MS-1 possessed markedly thrombolytic activity evaluated by plasminogen activator inhibitior-1, fibrin degradation products, and D-dimer levels using rats plasma, and recanalization rate by FeCl3-induced carotid artery thrombosis in mice. MS-1 exhibited strong antithrombotic activity in vitro and in vivo evaluated by the wet weighs and lengths of thrombus, and thrombus occlusion time by electrically-induced carotid artery thrombosis in rats. These results suggested that MS-1 could be a promising marine drug for prevention and therapy of thromboembolic disease.
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Lin, Peichun, Suhua Chen und Siyan Zhong. „Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans“. Foods 11, Nr. 12 (15.06.2022): 1771. http://dx.doi.org/10.3390/foods11121771.
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Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities. In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified. The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated. The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%. Its ability of binding bile acid was better than other five crude fucoidans. Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals. The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively. Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose. Three fucoidans can bind to bile acid, and ZF2 showed the best binding capability. In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability. ZF2 showed the best ability to reduce TC.
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van Vliet, Daan M., Yuemei Lin, Nicole J. Bale, Michel Koenen, Laura Villanueva, Alfons J. M. Stams und Irene Sánchez-Andrea. „Pontiella desulfatans gen. nov., sp. nov., and Pontiella sulfatireligans sp. nov., Two Marine Anaerobes of the Pontiellaceae fam. nov. Producing Sulfated Glycosaminoglycan-like Exopolymers“. Microorganisms 8, Nr. 6 (18.06.2020): 920. http://dx.doi.org/10.3390/microorganisms8060920.
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Recently, we isolated two marine strains, F1T and F21T, which together with Kiritimatiella glycovorans L21-Fru-ABT are the only pure cultures of the class Kiritimatiellae within the phylum Verrucomicrobiota. Here, we present an in-depth genome-guided characterization of both isolates with emphasis on their exopolysaccharide synthesis. The strains only grew fermentatively on simple carbohydrates and sulfated polysaccharides. Strains F1T, F21T and K. glycovorans reduced elemental sulfur, ferric citrate and anthraquinone-2,6-disulfonate during anaerobic growth on sugars. Both strains produced exopolysaccharides during stationary phase, probably with intracellularly stored glycogen as energy and carbon source. Exopolysaccharides included N-sulfated polysaccharides probably containing hexosamines and thus resembling glycosaminoglycans. This implies that the isolates can both degrade and produce sulfated polysaccharides. Both strains encoded an unprecedently high number of glycoside hydrolase genes (422 and 388, respectively), including prevalent alpha-L-fucosidase genes, which may be necessary for degrading complex sulfated polysaccharides such as fucoidan. Strain F21T encoded three putative glycosaminoglycan sulfotransferases and a putative sulfate glycosaminoglycan biosynthesis gene cluster. Based on phylogenetic and chemotaxonomic analyses, we propose the taxa Pontiella desulfatans F1T gen. nov., sp. nov. and Pontiella sulfatireligans F21T sp. nov. as representatives of the Pontiellaceae fam. nov. within the class Kiritimatiellae.
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Wang, Lei, Thilina U. Jayawardena, Hye-Won Yang, Hyo-Geun Lee und You-Jin Jeon. „The Potential of Sulfated Polysaccharides Isolated from the Brown Seaweed Ecklonia maxima in Cosmetics: Antioxidant, Anti-melanogenesis, and Photoprotective Activities“. Antioxidants 9, Nr. 8 (09.08.2020): 724. http://dx.doi.org/10.3390/antiox9080724.
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Sulfated polysaccharides prepared from marine algae are potential ingredients in nutraceutical, pharmaceutical, and cosmeceutical industries. In the present study, the antioxidant, anti-melanogenesis, and photoprotective effects of sulfated polysaccharides obtained from Ecklonia maxima (EMC) were investigated to evaluate their potential in cosmetic. EMC was successfully prepared through Celluclast-assisted extraction and ethanol precipitation, and it contained 79.88% of sulfated polysaccharides that with 69.37% carbohydrates and 10.51% sulfate. EMC effectively suppressed 2,2-azobis(2-amidinopropane) hydrochloride (AAPH)-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish. Furthermore, EMC significantly inhibited mushroom tyrosinase and reduced melanin synthesis in alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. In addition, EMC remarkably attenuated photodamage induced by UVB irradiation in vitro in human keratinocytes (HaCaT cells) and in vivo in zebrafish. Furthermore, EMC effectively inhibited wrinkle-related enzymes and improved collagen synthesis in UVB-irradiated human dermal fibroblasts (HDF cells). These results indicate that EMC possesses strong antioxidant, anti-melanogenesis, and photoprotective activities, and suggest that EMC may be an ideal ingredient in the pharmaceutical and cosmeceutical industries.
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Ustyuzhanina, Nadezhda, Maria Bilan, Elena Panina, Nadezhda Sanamyan, Andrey Dmitrenok, Eugenia Tsvetkova, Natalia Ushakova, Alexander Shashkov, Nikolay Nifantiev und Anatolii Usov. „Structure and Anti-Inflammatory Activity of a New Unusual Fucosylated Chondroitin Sulfate from Cucumaria djakonovi“. Marine Drugs 16, Nr. 10 (17.10.2018): 389. http://dx.doi.org/10.3390/md16100389.
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Fucosylated chondroitin sulfate CD was isolated from the sea cucumber Cucumaria djakonovi collected from the Avachinsky Gulf of the eastern coast of Kamchatka. Structural characterization of CD was performed using a series of non-destructive NMR spectroscopic procedures. The polysaccharide was shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n where about 60% of GlcA residues were 3-O-fucosylated, while another part of GlcA units did not contain any substituents. The presence of unsubstituted both at O-2 and O-3 glucuronic acid residues in a structure of holothurian chondroitin sulfate is unusual and has not been reported previously. Three different fucosyl branches Fucp2S4S, Fucp3S4S and Fucp4S were found in the ratio of 2:1:1. The GalNAc units were mono- or disulfated at positions 4 and 6. Anti-inflammatory activity of CD was assessed on a model of acute peritoneal inflammation in rats. About 45% inhibition was found for CD, while a structurally related linear chondroitin sulfate SS from cartilage of the fish Salmo salar demonstrated only 31% inhibition, indicating that the presence of sulfated fucosyl branches is essential for anti-inflammatory effect of chondroitin sulfates of marine origin.
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Ye, Meng-Qi, Chuan-Bo Jin, Xin-Jiang Liu, Xin-Yun Tan, Yu-Qi Ye und Zong-Jun Du. „Description and Genomic Characterization of Oceaniferula flavus sp. nov., a Novel Potential Polysaccharide-Degrading Candidate of the Difficult-to-Cultivate Phylum Verrucomicrobiota Isolated from Seaweed“. Marine Drugs 21, Nr. 1 (29.12.2022): 31. http://dx.doi.org/10.3390/md21010031.
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A novel strain, isolate 5K15T, which belongs to difficult-to-cultivate phylum Verrucomicrobiota, was recovered from kelp collected from Li Island, Rongcheng, China. The genome sequence of the strain (genome size 3.95 Mbp) showed the presence of four putative biosynthetic gene clusters (BGCs), namely, two terpene biosynthetic gene clusters, one aryl polyene biosynthetic cluster, and one type III PKS cluster. Genomic analysis revealed 79 sulfatase-encoded genes, 24 sulfatase-like hydrolase/transferase-encoded genes, and 25 arylsulfatase-encoded genes, which indicated the great potential of 5K15T to degrade sulfated polysaccharides. Comparative analysis of 16S rRNA gene sequence showed that the novel strain was most closely related to Oceaniferula marina N1E253T (96.4%). On the basis of evidence from a polyphasic study, it is proposed that the strain 5K15T (= KCTC 82748T = MCCC 1H00442T = SDUM 810003T) be classified as Oceaniferula flavus sp. nov. The strain has the ability of carbohydrate transport and metabolism. This ability allows it to survive in carbohydrate-rich materials such as kelp. It has the potential to be used in the marine drug industry using seaweed.
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Geetha Bai, Renu, und Rando Tuvikene. „Potential Antiviral Properties of Industrially Important Marine Algal Polysaccharides and Their Significance in Fighting a Future Viral Pandemic“. Viruses 13, Nr. 9 (13.09.2021): 1817. http://dx.doi.org/10.3390/v13091817.
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Over the decades, the world has witnessed diverse virus associated pandemics. The significant inhibitory effects of marine sulfated polysaccharides against SARS-CoV-2 shows its therapeutic potential in future biomedical applications and drug development. Algal polysaccharides exhibited significant role in antimicrobial, antitumor, antioxidative, antiviral, anticoagulant, antihepatotoxic and immunomodulating activities. Owing to their health benefits, the sulfated polysaccharides from marine algae are a great deal of interest globally. Algal polysaccharides such as agar, alginate, carrageenans, porphyran, fucoidan, laminaran and ulvans are investigated for their nutraceutical potential at different stages of infection processes, structural diversity, complexity and mechanism of action. In this review, we focus on the recent antiviral studies of the marine algae-based polysaccharides and their potential towards antiviral medicines.
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Mulloy, Barbara. „The specificity of interactions between proteins and sulfated polysaccharides“. Anais da Academia Brasileira de Ciências 77, Nr. 4 (Dezember 2005): 651–64. http://dx.doi.org/10.1590/s0001-37652005000400007.
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Sulfated polysaccharides are capable of binding with proteins at several levels of specificity. As highly acidic macromolecules, they can bind non-specifically to any basic patch on a protein surface at low ionic strength, and such interactions are not likely to be physiologically significant. On the other hand, several systems have been identified in which very specific substructures of sulfated polysaccharides confer high affinity for particular proteins; the best-known example of this is the pentasaccharide in heparin with high affinity for antithrombin, but other examples may be taken from the study of marine invertebrates: the importance of the fine structure of dermatan sulfate (DS) to its interaction with heparin cofactor II (HCII), and the involvement of sea urchin egg-jelly fucans in species specific fertilization. A third, intermediate, kind of specific interaction is described for the cell-surface glycosaminoglycan heparan sulfate (HS), in which patterns of sulfate substitution can show differential affinities for cytokines, growth factors, and morphogens at cell surfaces and in the intracellular matrix. This complex interplay of proteins and glycans is capable of influencing the diffusion of such proteins through tissue, as well as modulating cellular responses to them.
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Tang, Luying, Mengshi Xiao, Shenyuan Cai, Haijin Mou und Dongyu Li. „Potential Application of Marine Fucosyl-Polysaccharides in Regulating Blood Glucose and Hyperglycemic Complications“. Foods 12, Nr. 13 (05.07.2023): 2600. http://dx.doi.org/10.3390/foods12132600.
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Diabetes mellitus (DM) has become the world’s third major disease after tumors and cardiovascular disease. With the exploitation of marine biological resources, the efficacy of using polysaccharides isolated from marine organisms in blood glucose regulation has received widespread attention. Some marine polysaccharides can reduce blood glucose by inhibiting digestive enzyme activity, eliminating insulin resistance, and regulating gut microbiota. These polysaccharides are mainly fucose-containing sulphated polysaccharides from algae and sea cucumbers. It follows that the hypoglycemic activity of marine fucosyl-polysaccharides is closely related to their structure, such as their sulfate group, monosaccharide composition, molecular weight and glycosidic bond type. However, the structure of marine fucosyl-polysaccharides and the mechanism of their hypoglycemic activity are not yet clear. Therefore, this review comprehensively covers the effects of marine fucosyl-polysaccharides sources, mechanisms and the structure–activity relationship on hypoglycemic activity. Moreover, the potential regulatory effects of fucosyl-polysaccharides on vascular complications caused by hyperglycemia are also summarized in this review. This review provides rationales for the activity study of marine fucosyl-polysaccharides and new insights into the high-value utilization of marine biological resources.
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Jose, Geena Mariya, Mahadevan Raghavankutty und G. Muraleedhara Kurup. „Attenuation of hydrogenperoxide-induced oxidative damages in L929 fibroblast cells by sulfated polysaccharides isolated from the edible marine algae Padina tetrastromatica“. Journal of Bioactive and Compatible Polymers 34, Nr. 2 (März 2019): 150–62. http://dx.doi.org/10.1177/0883911519835144.
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Sulfated polysaccharides are polymers with potent biological roles such as antioxidant, anticancer, anti-inflammatory, and immunomodulatory activities. They are gaining significance in advanced healthcare research, since they can control oxidative cellular damages by arresting the reactive oxygen species. Hence, this study aims to evaluate the cellular antioxidant potential of sulfated polysaccharides isolated from the marine algae Padina tetrastromatica in L929 cells. The monosaccharide composition of sulfated polysaccharides was determined using high-performance liquid chromatography. The protective effect of sulfated polysaccharides on L929 fibroblast cells under H2O2-induced oxidative stress has been evaluated using MTT assay, acridine orange/ethidium bromide staining, flow cytometry, comet assay, DCFH-DA staining, fluorometry, and biochemical assays. Sulfated polysaccharides were found to contain glucose, fucose, xylose, and uronic acid. It was non-toxic to L929 cells and effectively increased the viability of mouse fibroblast cells under induced oxidative stress. Various cellular damages such as apoptosis, plasma membrane alterations, DNA damage, and lipid peroxidation induced by H2O2 have been attenuated by sulfated polysaccharides. This might be due to the reduction of intracellular reactive oxygen species level by sulfated polysaccharides as observed in DCFH-DA staining. Sulfated polysaccharides improved the activity of intracellular antioxidant status and glutathione system. The augmentation of endogenous antioxidant enzymes and glutathione system by sulfated polysaccharides can be the possible reason for the reduction of intracellular reactive oxygen species, prevention of apoptosis, and betterment of viability in L929 cells. The study confirms the potent antioxidant activity of the sulfated polysaccharides in addition to their structural and nutritional roles.
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Bento, Ananda de Araujo, Marianna Cardoso Maciel, Francisco Felipe Bezerra, Paulo Antônio de Souza Mourão, Mauro Sérgio Gonçalves Pavão und Mariana Paranhos Stelling. „Extraction, Isolation, Characterization, and Biological Activity of Sulfated Polysaccharides Present in Ascidian Viscera Microcosmus exasperatus“. Pharmaceuticals 16, Nr. 10 (03.10.2023): 1401. http://dx.doi.org/10.3390/ph16101401.
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Ascidians are marine invertebrates that synthesize sulfated glycosaminoglycans (GAGs) within their viscera. Ascidian GAGs are considered analogues of mammalian GAGs and possess great potential as bioactive compounds, presenting antitumoral and anticoagulant activity. Due to its worldwide occurrence and, therefore, being a suitable organism for large-scale mariculture in many marine environments, our main objectives are to study Microcosmus exasperatus GAGs regarding composition, structure, and biological activity. We also aim to develop efficient protocols for sulfated polysaccharides extraction and purification for large-scale production and clinical applications. GAGs derived from M. exasperatus viscera were extracted by proteolytic digestion, purified by ion-exchange liquid chromatography, and characterized by agarose gel electrophoresis and enzymatic treatments. Anticoagulant activity was evaluated by APTT assays. Antitumoral activity was assessed in an in vitro model of tumor cell culture using MTT, clonogenic, and wound healing assays, respectively. Our results show that M. exasperatus presents three distinct polysaccharides; among them, two were identified: a dermatan sulfate and a fucosylated dermatan sulfate. Antitumoral activity was confirmed for the total polysaccharides (TP). While short-term incubation does not affect tumor cell viability at low concentrations, long-term TP incubation decreases LLC tumor cell growth/proliferation at different concentrations. In addition, TP decreased tumor cell migration at different concentrations. In conclusion, we state that M. exasperatus presents great potential as an alternative GAG source, producing compounds with antitumoral properties at low concentrations that do not possess anticoagulant activity and do not enhance other aspects of malignancy, such as tumor cell migration. Our perspectives are to apply these molecules in future preclinical studies for cancer treatment as antitumoral agents to be combined with current treatments to potentiate therapeutic efficacy.
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Martins, Alice, Celso Alves, Joana Silva, Susete Pinteus, Helena Gaspar und Rui Pedrosa. „Sulfated Polysaccharides from Macroalgae—A Simple Roadmap for Chemical Characterization“. Polymers 15, Nr. 2 (12.01.2023): 399. http://dx.doi.org/10.3390/polym15020399.
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The marine environment presents itself as a treasure chest, full of a vast diversity of organisms yet to be explored. Among these organisms, macroalgae stand out as a major source of natural products due to their nature as primary producers and relevance in the sustainability of marine ecosystems. Sulfated polysaccharides (SPs) are a group of polymers biosynthesized by macroalgae, making up part of their cell wall composition. Such compounds are characterized by the presence of sulfate groups and a great structural diversity among the different classes of macroalgae, providing interesting biotechnological and therapeutical applications. However, due to the high complexity of these macromolecules, their chemical characterization is a huge challenge, driving the use of complementary physicochemical techniques to achieve an accurate structural elucidation. This review compiles the reports (2016–2021) of state-of-the-art methodologies used in the chemical characterization of macroalgae SPs aiming to provide, in a simple way, a key tool for researchers focused on the structural elucidation of these important marine macromolecules.
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Aitouguinane, Meriem, Zainab El Alaoui-Talibi, Halima Rchid, Imen Fendri, Slim Abdelkafi, Mohamed Didi Ould El-Hadj, Zakaria Boual et al. „Polysaccharides from Moroccan Green and Brown Seaweed and Their Derivatives Stimulate Natural Defenses in Olive Tree Leaves“. Applied Sciences 12, Nr. 17 (02.09.2022): 8842. http://dx.doi.org/10.3390/app12178842.
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This study aims to assess for the first time the ability of marine polysaccharides and their derivatives to stimulate natural defenses in olive tree leaves. Alginates, ALSM, and ALCM were isolated from the brown algae Sargassum muticum and Cystoseira myriophylloides, respectively. The OASM and OACM fractions were obtained after radical depolymerization of ALSM and ALCM, respectively. Three sulfated polysaccharides, arabinogalactans (AGB and AGP) and fucoidans (FUCCM) were extracted from the green alga Codium decorticatum and the brown seaweed C. myriophylloides, respectively. The extraction yield of different extracts was in the range of 0.82–32% and the average molecular weight (Mw) varied from 3000 Da to 2173 kDa. The M/G ratios of ALSM and ALCM were 0.87 and 1.12, respectively. FUCCM contained 53% of fucose and 12.83% of sulfates. The AGB and AGP fractions were characterized by the presence of a high degree of sulfation and protein (12–23% (w/w)) and were composed mainly of galactose, glucose, and arabinose. The aqueous saccharide solutions were applied to the leaf discs of the olive tree at 0.5 g/L, 1 g/L, and 2 g/L, for 24 h. These molecules triggered defense responses, by showing a differential capacity to induce the activity of the phenylalanine and tyrosine ammonia-lyase (TAL and PAL), polyphenols, and lignin contents in the leaves of the olive tree. Alginates and their derivatives as well as arabinogalactans exhibited an important induction of TAL activity compared to the PAL. The sulfated polysaccharides were more effective compared to the unsulfated polysaccharides (alginates) which were active at a low concentration. The optimum concentration for most of the studied elicitors was 2 g/L. These results suggest the valorization of these molecules derived from marine biomass as inducers of natural defenses of the olive tree to protect against phytopathogens in the context of sustainable development.
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Ficko-Blean, Elizabeth, Cecile Hervé und Gurvan Michel. „Sweet and sour sugars from the sea: the biosynthesis and remodeling of sulfated cell wall polysaccharides from marine macroalgae“. Perspectives in Phycology 2, Nr. 1 (01.05.2015): 51–64. http://dx.doi.org/10.1127/pip/2015/0028.
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Zahariev, Nikolay, Plamen Katsarov, Paolina Lukova und Bissera Pilicheva. „Novel Fucoidan Pharmaceutical Formulations and Their Potential Application in Oncology—A Review“. Polymers 15, Nr. 15 (29.07.2023): 3242. http://dx.doi.org/10.3390/polym15153242.
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Fucoidan belongs to the family of marine sulfated, L-fucose-rich polysaccharides found in the cell wall matrix of various brown algae species. In the last few years, sulfated polysaccharides have attracted the attention of researchers due to their broad biological activities such as anticoagulant, antithrombotic, antidiabetic, immunomodulatory, anticancer and antiproliferative effects. Recently the application of fucoidan in the field of pharmaceutical technology has been widely investigated. Due to its low toxicity, biocompatibility and biodegradability, fucoidan plays an important role as a drug carrier for the formulation of various drug delivery systems, especially as a biopolymer with anticancer activity, used for targeted delivery of chemotherapeutics in oncology. Furthermore, the presence of sulfate residues with negative charge in its structure enables fucoidan to form ionic complexes with oppositely charged molecules, providing relatively easy structure-forming properties in combination with other polymers. The aim of the present study was to overview essential fucoidan characteristics, related to its application in the development of pharmaceutical formulations as a single drug carrier or in combinations with other polymers. Special focus was placed on micro- and nanosized drug delivery systems with polysaccharides and their application in the field of oncology.
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Assreuy, Ana Maria S., Grazielle C. Pontes, Natalia V. F. C. Rodrigues, Daniel M. Gomes, Paulo A. Xavier, Glacio S. Araujo, Alexandre H. Sampaio, Benildo S. Cavada, Maria G. Pereira und Wladimir R. L. Farias. „Vascular Effects of a Sulfated Polysaccharide from the Red Marine Alga Solieria Filiformis“. Natural Product Communications 5, Nr. 8 (August 2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500825.
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Anticoagulant and antithrombotic properties of sulfated-polysaccharides (SP) from marine algae are extensively exploited. However, reports on the vascular effects of SP from red algae are rare in the literature. The polysaccharide from Solieria filiformis (Sf-SP) was isolated by ion exchange chromatography, analyzed by agarose gel electrophoresis and tested in male Wistar rats. The inflammation studies were performed using the paw-edema model and the relaxant activity in isolated aorta precontracted with phenylephrine. The anticoagulant effect was evaluated by the test of partial thromboplastin activation time. The SP (1 mg/kg) was not antiinflammatory, but induced acute edema with maximal activity at 30 min (0.35 ± 0.04 mL) compared to controls (0.05 ± 0.03 mL). Cumulative addition of Sf-SP in phenylephrine-contracted tissues produced relaxation with maximal inhibition of 69% (IC50 29.3 ± 9.0 μg/mL) at 300 μg/mL in comparison to controls (0.51 ± 0.09 g). Sf-SP also extended human plasma coagulation time by 2.1 times. These substances could be used as important tools for the study of vascular alterations.
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Salih, Abdalla E. M., Bathini Thissera, Mohammed Yaseen, Ahmed S. I. Hassane, Hesham R. El-Seedi, Ahmed M. Sayed und Mostafa E. Rateb. „Marine Sulfated Polysaccharides as Promising Antiviral Agents: A Comprehensive Report and Modeling Study Focusing on SARS CoV-2“. Marine Drugs 19, Nr. 8 (22.07.2021): 406. http://dx.doi.org/10.3390/md19080406.
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SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2) is a novel coronavirus strain that emerged at the end of 2019, causing millions of deaths so far. Despite enormous efforts being made through various drug discovery campaigns, there is still a desperate need for treatments with high efficacy and selectivity. Recently, marine sulfated polysaccharides (MSPs) have earned significant attention and are widely examined against many viral infections. This article attempted to produce a comprehensive report about MSPs from different marine sources alongside their antiviral effects against various viral species covering the last 25 years of research articles. Additionally, these reported MSPs were subjected to molecular docking and dynamic simulation experiments to ascertain potential interactions with both the receptor-binding domain (RBD) of SARS CoV-2’s spike protein (S-protein) and human angiotensin-converting enzyme-2 (ACE2). The possible binding sites on both S-protein’s RBD and ACE2 were determined based on how they bind to heparin, which has been reported to exhibit significant antiviral activity against SARS CoV-2 through binding to RBD, preventing the virus from affecting ACE2. Moreover, our modeling results illustrate that heparin can also bind to and block ACE2, acting as a competitor and protective agent against SARS CoV-2 infection. Nine of the investigated MSPs candidates exhibited promising results, taking into consideration the newly emerged SARS CoV-2 variants, of which five were not previously reported to exert antiviral activity against SARS CoV-2, including sulfated galactofucan (1), sulfated polymannuroguluronate (SPMG) (2), sulfated mannan (3), sulfated heterorhamnan (8), and chondroitin sulfate E (CS-E) (9). These results shed light on the importance of sulfated polysaccharides as potential SARS-CoV-2 inhibitors.
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Acharjee, Nivedita, und Tuhin Ghosh. „Structure Analysis of Sulfated Polysaccharides Extracted from Scinaia interrupta: A Experimental and Density Functional Theory Studies“. Asian Journal of Chemistry 32, Nr. 7 (2020): 1589–96. http://dx.doi.org/10.14233/ajchem.2020.22609.
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In present report, a combined experimental and theoretical study has been performed to address the isolation procedure and spectroscopic structure elucidation of polysaccharides such as xylomannan isolated from marine red algal source Scinaia interrupta. The structure of the polysaccharides obtained from the red algae of Scinaia interrupta has been studied from NMR, IR and GC-MS spectroscopy. The investigation revealed that red algae contained a backbone of α-(1→4)-linked D-mannopyranosyl residues substituted at 6-position with a single stub of β-D-xylopyranosyl residues. The major polysaccharide, which had 0.6 sulfate groups per monomer unit and an apparent molecular mass of 120 KDa. The backbone structure was optimized at DFT/B3LYP/6-311G(d,p) level of theory and GIAO-NMR studies were performed at B3LYP/6-311++G(2d,p) level of theory followed by mean absolute error calculations of the computed chemical shifts for two possible conformers resulting from the flipping of xylopyranosyl residue. The NMR calculations were in agreement with the experimental findings. The experimental 1H NMR chemical shifts were then correlated with the NBO, Merz Kollman (MK), ChelpG and Mulliken charges of the predicted conformer. A reasonable correlation with the experimental 1H NMR chemical shifts and the computed NBO charges with correlation coefficient of 0.906.
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Sucupira, Isabela D., Stephan Nicollas M. C. G. Oliveira, Gustavo R. C. Santos, Paulo A. S. Mourão und Roberto Fonseca. „Improved anticoagulant effect of fucosylated chondroitin sulfate orally administered as gastroresistant tablets“. Thrombosis and Haemostasis 117, Nr. 04 (2017): 662–70. http://dx.doi.org/10.1160/th16-09-0694.
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SummaryFucosylated chondroitin sulfate (FucCS) is a potent anticoagulant polysaccharide extracted from sea cucumber. Its anticoagulant activity is attributed to the presence of unique branches of sulfated fucose. Although this glycosaminoglycan exerts an antithrombotic effect following oral administration, high doses are necessary to achieve the maximum effect. The diminished activity of FucCS following oral administration is likely due to its degradation in the gastrointestinal tract and its limited ability to cross the intestinal cell membranes. The latter aspect is particularly difficult to overcome. However, gastroresistant tablet formulation may help limit the degradation of FucCS in the gastrointestinal tract. In the present work, we found that the oral administration of FucCS as gastroresistant tablets produces a more potent and prolonged anticoagulant effect compared with its administration as an aqueous solution, with no significant changes in the bleeding tendency or arterial blood pressure. Experiments using animal models of arterial thrombosis initiated by endothelial injury demonstrated that FucCS delivered as gastro-protective tablets produced a potent antithrombotic effect, whereas its aqueous solution was ineffective. However, there was no significant difference between the effects of FucCS delivered as gastroresistant tablets or as aqueous solution in a venous thrombosis model, likely due to the high dose of thromboplastin used. New oral anticoagulants tested in these experimental models for comparison showed significantly increased bleeding tendencies. Our study provides a framework for developing effective oral anticoagulants based on sulfated polysaccharides from marine organisms. The present results suggest that FucCS is a promising oral anticoagulant.Supplementary Material to this article is available online at www.thrombosis-online.com.
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Jabeen, Mehwish, Mélody Dutot, Roxane Fagon, Bernard Verrier und Claire Monge. „Seaweed Sulfated Polysaccharides against Respiratory Viral Infections“. Pharmaceutics 13, Nr. 5 (16.05.2021): 733. http://dx.doi.org/10.3390/pharmaceutics13050733.
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Respiratory viral infections have been a leading cause of morbidity and mortality worldwide. Despite massive advancements in the virology field, no specific treatment exists for most respiratory viral infections. Approved therapies against respiratory viruses rely almost exclusively on synthetic drugs that have potential side effects, restricting their use. This review aims to present natural marine sulfated polysaccharides possessing promising antiviral activity against respiratory viruses that could be a safe alternative to synthetic broad-spectrum antiviral drugs. The antiviral properties of marine sulfated polysaccharides are presented according to their mechanism of action on different types and strains of respiratory viruses, and the potential limits of their use are discussed.
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Zayed, Ahmed, Dalal A. Al-Saedi, Emmanuel Ofosu Mensah, Osman Nabayire Kanwugu, Parise Adadi und Roland Ulber. „Fucoidan’s Molecular Targets: A Comprehensive Review of Its Unique and Multiple Targets Accounting for Promising Bioactivities Supported by In Silico Studies“. Marine Drugs 22, Nr. 1 (30.12.2023): 29. http://dx.doi.org/10.3390/md22010029.
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Fucoidan is a class of multifunctional polysaccharides derived from marine organisms. Its unique and diversified physicochemical and chemical properties have qualified them for potential and promising pharmacological uses in human diseases, including inflammation, tumors, immunity disorders, kidney diseases, and diabetes. Physicochemical and chemical properties are the main contributors to these bioactivities. The previous literature has attributed such activities to its ability to target key enzymes and receptors involved in potential disease pathways, either directly or indirectly, where the anionic sulfate ester groups are mainly involved in these interactions. These findings also confirm the advantageous pharmacological uses of sulfated versus non-sulfated polysaccharides. The current review shall highlight the molecular targets of fucoidans, especially enzymes, and the subsequent responses via either the upregulation or downregulation of mediators’ expression in various tissue abnormalities. In addition, in silico studies will be applied to support the previous findings and show the significant contributors. The current review may help in understanding the molecular mechanisms of fucoidan. Also, the findings of this review may be utilized in the design of specific oligomers inspired by fucoidan with the purpose of treating life-threatening human diseases effectively.
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Jin, Jun-O., Dhananjay Yadav, Kajal Madhwani, Nidhi Puranik, Vishal Chavda und Minseok Song. „Seaweeds in the Oncology Arena: Anti-Cancer Potential of Fucoidan as a Drug—A Review“. Molecules 27, Nr. 18 (16.09.2022): 6032. http://dx.doi.org/10.3390/molecules27186032.
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Marine natural products are a discerning arena to search for the future generation of medications to treat a spectrum of ailments. Meanwhile, cancer is becoming more ubiquitous over the world, and the likelihood of dying from it is rising. Surgery, radiation, and chemotherapy are the mainstays of cancer treatment worldwide, but their extensive side effects limit their curative effect. The quest for low-toxicity marine drugs to prevent and treat cancer is one of the current research priorities of researchers. Fucoidan, an algal sulfated polysaccharide, is a potent therapeutic lead candidate against cancer, signifying that far more research is needed. Fucoidan is a versatile, nontoxic marine-origin heteropolysaccharide that has received much attention due to its beneficial biological properties and safety. Fucoidan has been demonstrated to exhibit a variety of conventional bioactivities, such as antiviral, antioxidant, and immune-modulatory characteristics, and anticancer activity against a wide range of malignancies has also recently been discovered. Fucoidan inhibits tumorigenesis by prompting cell cycle arrest and apoptosis, blocking metastasis and angiogenesis, and modulating physiological signaling molecules. This review compiles the molecular and cellular aspects, immunomodulatory and anticancer actions of fucoidan as a natural marine anticancer agent. Specific fucoidan and membranaceous polysaccharides from Ecklonia cava, Laminaria japonica, Fucus vesiculosus, Astragalus, Ascophyllum nodosum, Codium fragile serving as potential anticancer marine drugs are discussed in this review.
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Zayed, Ahmed, Jose Avila-Peltroche, Mona El-Aasr und Roland Ulber. „Sulfated Galactofucans: An Outstanding Class of Fucoidans with Promising Bioactivities“. Marine Drugs 20, Nr. 7 (24.06.2022): 412. http://dx.doi.org/10.3390/md20070412.
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Fucoidans encompass versatile and heterogeneous sulfated biopolysaccharides of marine origin, specifically brown algae and marine invertebrates. Their chemistry and bioactivities have been extensively investigated in the last few decades. The reported studies revealed diverse chemical skeletons in which l-fucose is the main sugar monomer. However, other sugars, i.e., galactose, mannose, etc., have been identified to be interspersed, forming several heteropolymers, including galactofucans/fucogalactans (G-fucoidans). Particularly, sulfated galactofucans are associated with rich chemistry contributing to more promising bioactivities than fucans and other marine polysaccharides. The previous reports in the last 20 years showed that G-fucoidans derived from Undaria pinnatifida were the most studied; 21 bioactivities were investigated, especially antitumor and antiviral activities, and unique biomedical applications compared to other marine polysaccharides were demonstrated. Hence, the current article specifically reviews the biogenic sources, chemistry, and outstanding bioactivities of G-fucoidans providing the opportunity to discover novel drug candidates.
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Besednova, Natalya N., Tatyana S. Zaporozhets, Boris G. Andryukov, Sergey P. Kryzhanovsky, Svetlana P. Ermakova, Tatyana A. Kuznetsova, Anastasia N. Voronova und Mikhail Y. Shchelkanov. „Antiparasitic Effects of Sulfated Polysaccharides from Marine Hydrobionts“. Marine Drugs 19, Nr. 11 (12.11.2021): 637. http://dx.doi.org/10.3390/md19110637.
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This review presents materials characterizing sulfated polysaccharides (SPS) of marine hydrobionts (algae and invertebrates) as potential means for the prevention and treatment of protozoa and helminthiasis. The authors have summarized the literature on the pathogenetic targets of protozoa on the host cells and on the antiparasitic potential of polysaccharides from red, brown and green algae as well as certain marine invertebrates. Information about the mechanisms of action of these unique compounds in diseases caused by protozoa has also been summarized. SPS is distinguished by high antiparasitic activity, good solubility and an almost complete absence of toxicity. In the long term, this allows for the consideration of these compounds as effective and attractive candidates on which to base drugs, biologically active food additives and functional food products with antiparasitic activity.
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Kamble, Manoj Tukaram, Tawut Rudtanatip, Chumporn Soowannayan, Boottoh Nambunruang, Seema Vijay Medhe und Kanokpan Wongprasert. „Depolymerized Fractions of Sulfated Galactans Extracted from Gracilaria fisheri and Their Antibacterial Activity against Vibrio parahaemolyticus and Vibrio harveyi“. Marine Drugs 20, Nr. 8 (23.07.2022): 469. http://dx.doi.org/10.3390/md20080469.
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Various seaweed sulfated polysaccharides have been explored for antimicrobial application. This study aimed to evaluate the antibacterial activity of the native Gracilaria fisheri sulfated galactans (NSG) and depolymerized fractions against the marine pathogenic bacteria Vibrio parahaemolyticus and Vibrio harveyi. NSG was hydrolyzed in different concentrations of H2O2 to generate sulfated galactans degraded fractions (SGF). The molecular weight, structural characteristics, and physicochemical parameters of both NSG and SGF were determined. The results revealed that the high molecular weight NSG (228.33 kDa) was significantly degraded to SGFs of 115.76, 3.79, and 3.19 kDa by hydrolysis with 0.4, 2, and 10% H2O2, respectively. The Fourier transformed spectroscopy (FTIR) and 1H− and 13C−Nuclear magnetic resonance (NMR) analyses demonstrated that the polysaccharide chain structure of SGFs was not affected by H2O2 degradation, but alterations were detected at the peak positions of some functional groups. In vitro study showed that SGFs significantly exerted a stronger antibacterial activity against V. parahaemolyticus and V. harveyi than NSG, which might be due to the low molecular weight and higher sulfation properties of SGF. SGF disrupted the bacterial cell membrane, resulting in leakage of intracellular biological components, and subsequently, cell death. Taken together, this study provides a basis for the exploitation and utilization of low-molecular-weight sulfated galactans from G. fisheri to prevent and control the shrimp pathogens.
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Ngo, Dai-Hung, und Se-Kwon Kim. „Sulfated polysaccharides as bioactive agents from marine algae“. International Journal of Biological Macromolecules 62 (November 2013): 70–75. http://dx.doi.org/10.1016/j.ijbiomac.2013.08.036.
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Muñoz-Ochoa, M., J. I. Murillo-Alvarez, Y. E. Rodríguez-Montesinos, G. Hernández-Carmona, D. L. Arvizu-Higuera, J. Peralta-Cruz und J. Lizardi-Mendoza. „ANTICOAGULANT SCREENING OF MARINE ALGAE FROM MEXICO, AND PARTIAL CHARACTERIZATION OF THE ACTIVE SULFATED POLYSACCHARIDE FROM Eisenia arborea“. CICIMAR Oceánides 24, Nr. 1 (04.06.2009): 15. http://dx.doi.org/10.37543/oceanides.v24i1.52.
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The in vitro anticoagulant activity of 41 water extracts of various seaweeds from Baja California Sur, Mexico was evaluated. In this study, nine extracts exhibited anticoagulant activity in the prothrombin time assay and 29 extracts were positive in the activated partial thromboplastin time assay. The water extract obtained at 25 °C from the brown seaweed Eisenia arborea was the most active in both assays, increasing the normal blood clotting-time over 300 s at 100 g mL-1. The fractionation of this extract by anion exchange chromatography yielded 3 fractions. Fraction 2 eluted with 1.0 M sodium chloride increased the clotting-time over 300 s in the activated partial-thromboplastin time assay at 5 g mL-1, being more active than sodium heparin. Chemical and spectroscopic analysis of fraction 2 showed it to be a sulfated heterofucan composed of 56.2 % ± 0.1% of total sugars and 45 % of sulfates.The neutral sugar constituents of the active heterofucan was determined to be 47.6 % fucose, 35.5 % xylose and 16.9 % rhamnose, with substitutions of sulfate groups at C-4 (axial), and minor substitutions at C-2 and-or C-3. Monitoreo de anticoagulantes en algas marinas de México y caracterización parcial de polisacáridos sufatados activos de Eisenia arborea Se evaluó la actividad anticoagulante in vitro de 41 extractos acuosos de diversas algas de Baja California Sur. Nueve extractos exhibieron actividad anticoagulante en el ensayo de tiempo de protrombina y 29 extractos fueron activos en el ensayo de tiempo de tromboplastina parcial activada. El extracto acuoso de Eisenia arborea obtenido a 25 °C fue el más activo en ambos ensayos, incrementando el tiempo normal de coagulación a más de 300 s, a una concentración de 100 g mL-1. El fraccionamiento de este extracto por cromatografía de intercambio iónico resultó en 3 fracciones. La fracción 2 eluída con cloruro de sodio 1.0 M incrementó el tiempo de coagulación a más de 300 s en el ensayo de tiempo de la tromboplastina parcial activada a una concentración de 5 g mL-1; resultando más activa que el control positivo (heparina de sodio). Los análisis químicos y espectroscópicos mostraron que la fracción 2 era un heterofucano sulfatado, compuesto por 56.2 % ± 0.1 % de azúcares totales y 45% de sulfatos. Los azúcares neutros constituyentes del heterofucano activo fueron determinados como 47.6 % fucosa, 35.5 % xylosa y 16.9 % ramnosa, con sustituciones de los grupos sulfato en C-4 (axial) y sustituciones menores en C-2 y/o C-3.