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1
Last, Kim Sven. „Photoperiodism in the semelparous polychaete Nereis virens sars“. Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324943.
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O’Brien, Conor Savage. „Evolution of Photoperiodism in the Threespine Stickleback Gasterosteus aculeatus“. Thesis, University of Oregon, 2011. http://hdl.handle.net/1794/12104.
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xvi, 112 p. : ill. (some col.)In seasonal environments, the ability to take advantage of the favorable seasons and avoid or mitigate the effects of the unfavorable ones is essential for organismal fitness. Many polar and temperate organisms use photoperiod (length of day) to time seasonal life history events because photoperiod's regular annual cycle makes it a very reliable indicator of seasonality. This reliability allows organisms to anticipate and properly prepare for seasonal change. Although photoperiodism is widespread in polar and temperate vertebrates, little is known relative to invertebrates regarding how its use varies with environment and this method's underlying genetic and physiological basis. This dissertation is focused on demonstrating the proper methodology for the study of photoperiodism and establishing the threespine stickleback as a model of vertebrate photoperiodism. Chapter I is an introduction to photoperiodism, how it is influenced by environment, the physiological basis of its output, and a summary of the chapters that follow. Chapter II explains an analytical method to test for causality and applies this method to data that have been interpreted as evidence that the circadian clock is causally involved in photoperiodism. Chapter III describes the photoperiodic response of threespine stickleback Gasterosteus aculeatus populations from two latitudes. These results are used to inform an empirical examination of the previously described assertion that the circadian clock is causally involved in photoperiodism. Chapter IV examines the physiological basis of early photoperiodic response using the threespine stickleback as a model teleost fish. Chapter V summarizes the previous chapters, describes their significance, and suggests future research directions. This dissertation includes both previously published and co-authored material. Supplementary Excel files demonstrating the analyses used in Chapter III are also included in this dissertation.
Committee in charge: Eric Johnson, Chairperson; William Cresko, Advisor; William Bradshaw, Member; Judith Eisen, Member; Patricia McDowell, Outside Member
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Robertson, Carol Elaine. „The use of quantitative RT-PCR techniques to examine the expression of PHY-genes : the role of phytochrome A in the photoperiodic induction of flowering in the long-day-plant Sinapis alba and the short-day-plant Pharbitis nil“. Thesis, University of Reading, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282609.
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4
Sáenz, de Miera Cristina. „The role of photoperiodic history and internal long-term timing in seasonal neuroendocrinology“. Thesis, University of Aberdeen, 2014. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=225273.
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Seasonal physiology has evolved as an adaptive strategy to changing environments with daylength (photoperiod) used as the predominant environmental cue to suit breeding and other functions to the external season. However, seasonal physiological state is determined not only by the photoperiod that is currently in effect but also by the animal's history, allowing changes in physiology in anticipation to the seasons. Many mammals and birds show internally timed, long-term (circannual) changes in seasonal physiology, synchronised to the seasons by changing photoperiods. The importance of history-dependent photoperiodic programming applies also to puberty attainment in juvenile animals, timed by the photoperiod received by the mother during gestation. In this project I investigated the effects of both types of history-dependent timing on the neuroendocrine pathways for photoperiodic regulation of seasonal physiology. In mammals, photoperiod is transmitted via the pineal hormone melatonin, which acts on the pars tuberalis (PT) to regulate thyrotropin (TSH) expression and in turn controls seasonal physiology via effects on the hypothalamic synthesis of type 2 and 3 thyroid hormone deiodinases (Dio2 and Dio3), and thus the local regulation of thyroid hormone metabolism, and downstream changes in hypothalamic neuropeptidergic signalling. Using two circannual species, the Soay sheep (Ovis aries) –a short-day breeder – and the European hamster (Cricetus cricetus) – a long-day breeder – exposed to constant photoperiodic conditions, my findings reveal that in both models, in the absence of seasonal cues, internal circannual timing is initiated at the PT control of TSH and transmitted to the regulation of hypothalamic T3 regulation and neuropeptides. Siberian hamsters (Phodopus sungorus) were placed under different photoperiods during gestation and transferred to a photoperiod of intermediate duration at weaning. Reproductive activation under these conditions was dependent upon early life exposure and this effect controls history-dependent changes in hypothalamic deiodinases. Interestingly, the gestational experience was reflected in PT TSH expression and Dio2 expression as early as birth time. The same prenatal effects were observed in a strain of seasonal mice, (Mus musculus molossinus). Overall my dissertation has established that: i) both the circannual and the melatonin signals converge on TSH expression to synchronise seasonal biological activity; ii) the photoperiodic pituitary-hypothalamic network is programmed by prenatal experience; and iii) this pathway is already functional before birth. Overall, my results highlight the PT as a conserved central site in mammals for the integration of multiple seasonal cues which via differential control of thyroid hormone levels in the hypothalamus dictates the timing in seasonal physiology.
5
Holm, Karl. „Studies on Natural Variation and Evolution of Photoperiodism in Plants“. Doctoral thesis, Uppsala universitet, Evolutionär funktionsgenomik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-119269.
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Photoperiodism refers to the organism’s ability to detect and respond to seasonal changes in the daily duration of light and dark and thus constitutes one of the most significant and complex examples of the interaction between the organism and its environment. This thesis attempts to describe the prevalence of variation in a photoperiodic response, its adaptive value, and its putative genetic basis in a common cruciferous weed, Capsella bursa-pastoris (Brassicaceae). Furthermore, the thesis presents a first comprehensive comparative overview of the circadian clock mechanism in an early land plant, Physcomitrella patens (Bryophyta), thus providing insights into the evolution of the plant circadian system. In an introductory survey of global gene expression changes among early- and late flowering accessions of C. bursa-pastoris we found an enrichment of genes involved in photoperiodic response and regulation of the circadian clock. Secondly, by phenotyping circadian rhythm variation in a worldwide sample of accessions with known flowering time, we detected robust latitudinal clines in flowering time and circadian period length, which constitute strong indications of local adaptation to photoperiod in the shaping of flowering time variation in this species. In an attempt to elucidate putative genetic causes for the correlated variation between circadian rhythm and flowering time, we found that sequence variation and diverged expression in components regulating light input to the clock, PHYTOCHROME B (PHYB) and DE-ETIOLATED 1 (DET1) make them strong candidate genes. Finally, we present a comparative study of circadian network topology in the moss P. patens. Phylogenetic analyses and time series expression studies of putative clock homologues indicated that several core clock genes present in vascular plants appeared to be lacking in the moss. Consequently, while the clock mechanism in higher plants constitutes at least a three-loop system of interacting components, the moss clock appears to comprise only a single loop. We conclude that C. bursa-pastoris is a highly suitable model system for the further elucidation of the molecular variation that influences adaptive change in natural plant populations. Furthermore, we believe that the continuing study of the seemingly less complex circadian network of P. patens not only can provide insights into the evolution of the plant circadian system, but also may help to clarify some of the remaining issues of the circadian clock mechanism in higher plants.
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Tan, Ying. „Neurospora crassa - A model system for photoperiodism and circadian rhythm research“. Diss., lmu, 2003. http://nbn-resolving.de/urn:nbn:de:bvb:19-47324.
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7
Andersson, Håkan. „Photoperiodism in pigs : studies on timing of male puberty and melatonin /“. Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 2000. http://epsilon.slu.se/v90.pdf.
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8
Yang, Jingying. „Photoperiodism and endocrine control of reproduction in the Turkey (Meleagris gallopavo) /“. The Ohio State University, 1998. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487952208107354.
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9
LUBBERS, EDWARD LAWRENCE. „CHARACTERIZATION AND INHERITANCE OF PHOTOPERIODISM IN GUAR, CYAMOPSIS TETRAGONOLOBA (L.) TAUB“. Diss., The University of Arizona, 1987. http://hdl.handle.net/10150/184079.
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Three hundred and thirty lines of guar (Cyamopsis tetragonoloba (L.) taub.) were planted in five locations throughout central and southwestern United States to find diverse photoperiod response types for closer physiological and genetic study. Dates of planting studies were done in 1982 and 1983 in hopes that the photoperiod responses would be obvious in field conditions but they were not. The 1982 dates of planting studies in Arizona, Kansas, and Texas indicated that the date of planting was more important than the selection of cultivar in expectations of high yield even though cultivar selection was very important. The 1983 dates of planting experiment in Tucson, Arizona showed suggestions that photoperiod existed in guar but it took controlled, greenhouse conditions to characterize photoperiodism in guar and to be able to conduct genetic analysis. In greenhouse studies, guar was found to be a quantitative short-day plant, the initiation of buds and floral development were accelerated under short-day conditions. Six guar lines were characterized for the critical photoperiod in days from first true leaf to the first floral bud and from first floral bud to the first flower. No effect of photoperiod on the growth and development from emergence to the first true leaf was observed. The critical photoperiod for days from first true leaf to first bud for the lines are as follows: PI217925-1-1, Mesa, and Mills are between 14 and 15 hours, Kinman and SEAH-90 are between 13 and 14 hours, and PI217925-2 is between 12 and 13 hours. The critical photoperiod for days from first floral bud to first flower for the lines are: PI217925-1-1, Mesa, Kinman, and PI217925-2 are between 12 and 13 hours, SEAH-90 is between 13 and 14 hours, and Mills is day-neutral. Different photoperiodic responses occur for days from first true leaf to first floral bud and days from first floral bud to first flower. This follows a proposed genetic system of photoperiodic actions that has genes for photoperiod sensitivity, short-day versus long-day reaction, critical photoperiod, and genes for the amount of time delay for each developmental stage. The segregations of the guar crosses were explained by the model.
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Mathias, Derrick Kenneth. „The evolution of a seasonal adaptation in the pitcher-plant mosquito, Wyeomyia smithii /“. view abstract or download file of text, 2006. http://proquest.umi.com/pqdweb?did=1276394641&sid=1&Fmt=2&clientId=11238&RQT=309&VName=PQD.
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Thesis (Ph. D.)--University of Oregon, 2006.Typescript. Includes vita and abstract. Includes bibliographical references (leaves 96-103). Also available for download via the World Wide Web; free to University of Oregon users.
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Mendez, Jocelyn V. „Comparison of photoperiod-sensitive and photoperiod-insensitive oat genotypes“. Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23921.
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Two experiments were conducted in 1994 and 1995 to compare the field performance of photoperiod sensitive (PS) and photoperiod insensitive (PI) oat (Avena sativa L) genotypes. In the first experiment, bulks of PS and PI lines from four covered oat crosses, as well as the cultivars Sylva, Newman, the PI naked oat cultivar AC Lotta and the breeding line NO820-3L were tested at seven locations across Quebec and Ontario. The second experiment was a seeding. date trial performed at Ste-Anne-de-Bellevue in which individual PS and PI lines of three covered oat crosses were utilized. In the multi-location experiment, the PI genotypes generally reached the different growth stages significantly earlier. At Normandin, Quebec the northernmost site, PS genotypes had consistently higher yields than the PI genotypes. At one southern site (Elora, Ontario), PI bulks yielded more than their PS counterparts. The PS genotypes sometimes had higher leaf numbers in most of the seven locations. In the experiment with different seeding dates, the PS lines had significantly higher numbers of leaves, biomass, grain weight per plant, harvest index, and yield compared to the PI lines. The response of the PS and PI lines to delayed sowing was similar. There was a progressive reduction in the number of days to reach Zadoks' 30, heading, and maturity: and a reduction in height, above-ground biomass, and yield with delayed sowing. Number of culms, leaves, and fertile culms, and harvest index were not affected by delayed sowing. Based on the results of these experiments, it seems that the Di1 gene does not confer any consistent yield advantage or disadvantage. Nevertheless, there may be some northern environments for which photoperiod sensitive oat genotypes are better suited.
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De, Caluwe Joelle. „Modeling the plant circadian clock: a study of light, photoperiodism, and growth“. Doctoral thesis, Universite Libre de Bruxelles, 2017. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/251373.
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Le travail présenté dans cette thèse consiste en la création et l'étude des propriétés d'un nouveau modèle computationnel de l'horloge circadienne végétale et de certains processus physiologiques qui en dépendent.L'horloge circadienne est un rythme endogène d'une période d'environ 24 heures que possèdent la plupart des êtres vivants. Il est généré au niveau moléculaire par des boucles de rétroaction transcriptionnelles, traductionnelles et/ou post-traductionnelles. L'horloge permet aux organismes de s'a- dapter à leur environnement. L'horloge des plantes se distingue par un nombre important de composants (gènes et protéines) dont la majorité sont régulés par la lumière.Dans un premier temps, un nouveau modèle computationnel qui combine une structure complexe et hautement interconnectée avec un nombre réduit d'équations et de paramètres est construit. Ce modèle reproduit correctement les profils d'expression des gènes de l'horloge du type sauvage ainsi que les altérations provoquées par une perte de fonction de chacun de ces gènes. Plusieurs extensions modélisant des processus physiologiques dépendant de l'horloge, à savoir la croissance de l'hypocotyle et la régulation de la floraison, sont également testées.Ensuite, la réponse particulièrement complexe de l'horloge végétale à la lumière est explorée en détail afin de déterminer l'utilité de multiples récepteurs lumineux. Pour ce faire, l'entraînement de l'oscillateur par des cycles jour-nuit de durée différente de 24 heures est mesuré et les différents comportements observés (entraînement périodique, quasipériodicité, chaos) sont caractérisés. Les simulations suggèrent que les multiples senseurs lumineux permettent d'allier une grande flexibilité et une résistance aux effets des fluctuations rapides de luminosité, améliorant ainsi l'adaptation des plantes à l'environnement.Enfin, plusieurs hypothèses permettant de rendre compte des différences observées entre l'horloge des racines et celle des feuilles sont explorées, et différents mécanismes de synchronisation entre ces deux oscillateurs sont testés.The circadian clock is an endogenous timekeeper with a period of around 24 hours, found in most living beings, which helps organisms adapt to their environment by anticipating daily and seasonal variations. It originates at the molecular level, from transcriptional-translational feedback loops between a small number of genes.In this thesis, a computational model of the plant circadian oscillator is built based on current knowledge of the underlying genetic network. This network is highly complex and interconnected, but the new model needs only a small number of equations and parameters to accurately predict the expression profiles of the main clock genes in various light conditions, as well as the defects associated with a loss of function in those genes. Clock-regulated processes such as hypocotyl growth and flowering are also reproduced with good accuracy. One of the particularities of the plant clock is a large number of light-sensitive components. A study of the role of those multiple light sensors on the entrainment properties of the clock is presented. It uses the newly built model to subject the clock to a very large range of conditions and generate theoretical light-insensitive mutants. The combination of an intricate oscillator and a multiplicity of light sensors makes the plant clock highly flexible, able to adapt to a wide range of conditions but resistant to the disrupting effects of random fluctuations.Preliminary steps towards a more realistic depiction of the plant clock as multiple interacting oscillators are taken. These include modeling a heterogeneous population by changing parameter values, modifying the model to account for known differences between the clocks of the roots and shoots, and testing possible synchronizing mechanisms between those two organs.
Doctorat en Sciences
info:eu-repo/semantics/nonPublished
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Emerson, Kevin James. „Evolutionary and physiological genetics of biological timing /“. Connect to title online (Scholars' Bank) Connect to title online (ProQuest), 2009. http://hdl.handle.net/1794/10286.
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14
Duncan, Neil. „Photoperiodic manipulation and its use in the all year round production of Atlantic salmon, Salmo salar“. Thesis, University of Stirling, 1996. http://hdl.handle.net/1893/21632.
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The Atlantic salmon (Salmo salar) has two developmental processes which are clearly seasonal; smoltification is restricted to the spring and maturation to late autumn. In the farming industry the seasonality of juvenile production is primarily responsible for the seasonal production in market-sized fish. Both smoltification and maturation appear to be controlled by mechanisms timed by photoperiod. This thesis investigates the effects of photoperiod on smoltification and maturation. The performance of both out-of-season eggs and particularly smolts are closely examined and the possible application of photoperiod techniques in the industry discussed. Both potential SI and S2 parr were subjected to natural photoperiods or a range of artificial photoperiods under constant and ambient temperature conditions. S I and S2 smolts reared under natural photoperiods were transferred to sea water during April and May. A 2-3 month period of short days followed by a period of long days was observed to advance smoltification; 0+ and 1+ smolts were transferred to sea during December (4 month advance) and October (6 month advance), respectively. A 12 month seasonal photocycle delayed by 3 months delayed smoltification by 2 months. Out-of-season smolts were produced in January, March, April, May, June, July, October, November and December. All groups of out-of-season smolts exhibited the same growth potential as natural smolts. The different transfer date of out-of-season smolts therefore resulted in different harvest periods compared to natural smolts. The age at maturity of out-of-season smolts was positively related to the length of the seawater growing period prior to the completion of maturation. Out-of-season smolts exhibited increased maturation in association with an increased size (compared to natural smolts) for a particular time of year. These large maturing fish were generally harvested as superior salmon prior to flesh deterioration. Mortality during the first month in sea water was significantly higher in a number of out-of-season smolt groups compared to natural smolts. This was considered to be related to site-specfic environmental conditions rather than the smoltification process. Smoltification was also examined under constant photoperiods and a 4 week period of short daylengths. Dissociation and suppression of certain aspects of smoltification were observed. The different aspects of smoltification: the development of bimodality, hypoosmoregulatory ability, decrease in condition and smolt coloration appeared to be controlled by different independent mechanisms. The development of bimodality, hypoosmoregulatory ability and possibly coloration appeared to be controlled by independent endogenous rhythms. The decrease in condition factor, associated with the parr-smolt transformation required a period of short day length greater than 2 months followed by a period of long daylength. Smoltification and maturation were examined in salmon retained in constant 1DOC borehole water. Smolts held in fresh water grew steadily and matured producing eggs and sperm both in- and out-of-season. There was, however, a period of increased mortality after smoltification and egg quality was reduced especially in fish subjected to photoperiod manipulation. The salmon responded to photoperiodic manipulation. Abrupt changes in photoperiod advanced spawning by 12 weeks and a reciprocal seasonal photoperiod (6 month out of phase with a natural photoperiod) advanced spawning by 22 weeks. The maturation process in the Atlantic salmon would appear to be controlled by similar mechanisms to those described for the rainbow trout. Freshwater holding conditions appeared to decrease the age at maturity and reduce egg quality. The progeny of freshwater broodstock (FI generation) successfully completed smoltification and seawater transfer. Photoperiodic manipulation can be used to produce out-of-season smolts and eggs. Through the use of photoperiod, farms could increase and target production. This could remove the seasonality from the production cycle and help stabilise the pricing structure for market salmon.
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HAUSER, URSULA ESTHER. „PHOTOPERIODIC REGULATION OF THE FEMALE REPRODUCTIVE SYSTEM IN THE SYRIAN HAMSTER (OVARY, PINEAL, HORMONES, PITUITARY)“. Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183862.
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Female golden Syrian hamsters are seasonally breeding animals, capable of maintaining continuous estrous cycles when the daylength is 12.5 hrs. or longer. In shorter photoperiod (SP) the ovaries of anovulatory animals are characterized by few small growing follicles, an absence of corpora lutea and extensive hypertrophied interstitium. Steroid-histochemical studies revealed that enzymes related to steroidogenesis show intense activity in the interstitial tissue of SP-exposed animals. The major objectives of these studies were to examine SP-induced hormonal and ovarian changes which occur prior to onset of the acyclic condition in inbred LSH/SsLak hamsters. Other experiments explored hormonal changes in the absence of ovarian hormones and the interaction of SP and steroids. Initial results revealed that the LSH/SsLak hamster ceased estrous cyclicity between 14 and 31 days of SP exposure, a response far more uniform than generally seen in outbred hamsters. Experiments carried out in SP-exposed cyclic animals indicated that the secondary FSH surge and follicular recruitment were not affected by SP treatment, follicular recruitment were not affected by SP treatment, and that no major changes in gonadotropin levels and ovarian steroids were present on individual days of the estrous cycle. Once the animals were anestrous, daily gonadotropin surges were present and pituitary gonadotropin contents increases. Serum PRL levels showed a slight, yet significant, decrease in SP cycling animals followed by a further reduction in pituitary and serum levels after animals ceased cycling. Medial basal hypothalamic LHRH contents did change in SP, yet there was a significant increase in the preoptic area, and LHRH became significantly elevated in both areas after the animals became anestrous. Ovarian histology revealed fewer corpora lutea and a slight shift from healthy to atretic antral follicles. Experiments carried out in ovariectomized SP-treated animals showed that serum gonadotropin levels were significantly reduced, and that estrogen treatment was either equally or less effective in reducing levels in SP animals. In contrast, PRL levels did not change and responded in a dose dependent way to estrogen treatment. Although the studies yielded no definite proof, the result suggest that SP impairs the maintenance of follicular growth leading eventually to the acyclic state.
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Murphy, Andrew James. „Calmodulin as a regulator of circadian clock function and photoperiodic flowering in Arabidopsis thaliana“. Thesis, University of Exeter, 2009. http://hdl.handle.net/10036/99353.
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Discrete changes in the amplitude, frequency, and cellular localisation of calcium ion (Ca2+) transients encode information about numerous stimuli and function to mediate stimulus-specific responses. Cytoplasmic Ca2+ (Ca2+cyt) undergoes circadian oscillations in concentration that appear to be under the control of the same endogenous oscillator that regulates expression of genes in the photoperiodic-flowering pathway. It is currently not known whether these circadian [Ca2+cyt] oscillations are biochemical artefacts or are decoded and function to transduce clock dependent responses. Calmodulin (CaM) is a primary node in Ca2+ signalling in plants and as such is a promising target for investigating the role of Ca2+ in clock-controlled processes. In this study, Arabidopsis thaliana were treated with experimentally validated concentrations of pharmacological CaM inhibitors. Under inductive photoperiods (16 h light : 8 h dark), CaM inhibition was found to increase developmental flowering time, whilst under non-inductive photoperiods no such changes were evident. Inhibition of CaM led to changes in expression of the key clock gene TIMING OF CAB EXPRESSION 1 and flowering time genes, CONSTANS and FLOWERING LOCUS T and removed repression of flowering in darkness. These observations are consistent with CaM modulating the activity of the putative clock component GIGANTEA and the proteasomal targeting protein SUPPRESSOR OF PHYA-105. Due to the unwanted side effects often generated by chemical CaM inhibitors, a peptide inhibitor of CaM comprising a green fluorescent protein / calspermin fusion and labelled smGN was developed. Surface plasmon resonance analysis and affinity chromatography showed smGN to have extremely high selectivity for, and affinity to, CaM and to function as a powerful inhibitor of CaM in vitro. Further work on the methodology used to deploy smGN as a recombinant alternative to chemical CaM inhibitors in planta is also described.
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DePatie, Nicholas. „Expression of Core Circadian Clock Genes Unable to Explain Changes in the Photoperiodic Timer Across Latitudinal and Altitudinal Gradients in Wyeomyia smithii“. Thesis, University of Oregon, 2018. http://hdl.handle.net/1794/23149.
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Photoperiodism is the ability of plants and animals to utilize day length or night length to mitigate seasonal exigencies. The circadian clock allows organisms to organize daily demands. Both process are set by light, and for more than 80 years a functional relationship has been pursued. Previous experiments have revealed, through phenotypic expression, that the daily circadian clock and seasonal photoperiodic timer have evolved independently, yet molecular evidence is lacking. Herein, we use the mosquito, Wyeomyia smithii, to understand the relationship between the photoperiodic response, diapause, and the daily circadian clock. We measured variation in the formal properties of the core circadian clock over a latitudinal and altitudinal gradient which we compare to the critical photoperiod, a measure of diapause, over the same geographic gradient. We found that there is no correlation with any of the formal properties of the core circadian clock and critical photoperiod, indicating independent evolution.
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Lee, Chee Wee. „The effects of photoperiod on reproductive performance of turkey males /“. Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63294.
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McStay, Elsbeth. „Photoperiod regulation of molecular clocks and seasonal physiology in the Atlantic salmon (Salmo salar)“. Thesis, University of Stirling, 2012. http://hdl.handle.net/1893/11012.
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Recent years have seen considerable advances in the study of biological rhythms and the underlying molecular mechanisms that drive the daily and seasonal physiology of vertebrates. Amongst teleosts the majority of work in this field has focused on the model species the zebrafish to characterise clock genes and the molecular feedback loop that underpins circadian rhythms and physiology. Daily profiles of clock gene expression in a wide variety of tissues and cell types are now relatively well described. However the zebrafish is a tropical species that does not display distinct seasonality and therefore may not be the species of choice to investigate the entrainment of circannual physiology. In contrast, Atlantic salmon is a highly seasonal teleost that displays considerable temporal organisation of most physiological processes. In salmonids photoperiod is widely known to synchronise physiology to the environmental conditions and as such photoperiod manipulation is routinely used by the salmon industry throughout the production cycle to control and manipulate spawning, smoltification and puberty. Previous studies in salmonid species have already identified a set of clock genes that are linked to these seasonal physiological processes. However, to date, the molecular mechanisms regulating daily and seasonal physiology are largely unknown despite the strong commercial relevance in the Atlantic salmon. In the Atlantic salmon, Davie et al (2009) was the first to report the photoperiod dependent circadian expression of clock genes (Clock, Bmal and Per2 and Cry2) in the brain of the Atlantic salmon. In the same investigation the expression of clock genes was reported in a wide variety of peripheral tissues, however 24h profiles of expression in peripheral tissues were not characterised. In order to examine further the role of seasonal photoperiod on the circadian expression of clock genes, the present work first aimed to characterise diel profiles of Clock, Per1 and Per 2 expression in the brain together with plasma melatonin levels in II Atlantic salmon acclimated to either long day (LD), short day (SD), 12L:12D (referred to as experiment 1 throughout) and SNP (referred to as experiment 2 throughout). Photoperiod dependent clocks were also investigated in peripheral tissues, namely in the fin and liver. Results showed circadian profiles of melatonin under all photoperiods. In experiment 1 both Clock and Per2 displayed significant circadian expression in fish exposed to LD. This is in contrast to previous results where rhythmic clock gene expression was observed under SD. In addition, clock gene expression differed in response to experimental photoperiod in the liver, and diel rhythm differed to that of the brain. No rhythmic expression was observed in the fin. Levels of plasma melatonin exhibited a circadian rhythm peaking during the nocturnal phase as expected. However the amplitude of nocturnal melatonin was significantly elevated under LD (experiment 1) and the SNP long day photoperiod and 2010 autumnal equinox samples (experiment 2). Overall results from these experiments suggested that the control of clock gene expression would be photoperiod dependent in the brain and the liver however photoperiod history is also likely to influence clock gene expression. Interestingly, the gradual seasonal changes in photoperiod under SNP did not elicit similar profiles of clock gene expression as compared to experimental seasonal photoperiods and clock gene expression differed between experimental photoperiod and SNP treatments. In experiment 2 significant seasonal differences were also observed in the amplitude of individual clock gene expression. The mechanisms underlying this and potential impact on seasonal physiology are unknown. Developmental changes such as the smoltification process or abiotic factors such as temperature or salinity should be further investigated. In mammals previous work has focused on the molecular switch for photoperiod response and regulation of thyroid hormone bioactivity via deiodinase mediated conversion of T4 to the biologically active form T3. In mammals and birds expression of key seasonal molecular markers i.e. Tsh, Eya3 and Dio2, are up-regulated hours after exposure to the first LD and III persist under chronic LD conditions. In order to confirm the involvement of these genes in the seasonal photoperiodic response in salmon, a microarray study was first carried out. Results displayed transcriptome level differences in the seasonal expression of a wide variety of target genes including Eya3 and Dio1-3 in relation to LD and SD photoperiod suggesting that these genes may have a conserved role in salmon. qPCR validations of selected genes of interest were then performed (Dio1, Dio2 and Dio3, Eya3 and Tshover diel cycles in fish exposed to LD and SD photoperiod (autumn acclimated fish). In addition an unrelated qPCR study was undertaken in salmon parr acclimated to LD, 12L12D and SD photoperiod (spring acclimated fish)(Dio2, Eya3 and Tsh. Consistent with findings obtained in other vertebrate species, circadian expression of Dio2 was observed under LD. However expression of Eya3 and Tsh appeared to be dependent on photoperiod history prior to acclimation to the experimental photoperiods as already suggested for clock gene expression in this thesis. This is potentially a consequence of direct regulation by clock genes. To our knowledge, this is the first report on the expression of key molecular components that drive vertebrate seasonal rhythms in a salmonid species. The thesis then focused on another key component of the photoneuroendocrine axis in fish, the pineal organ. In the Atlantic salmon, as in other teleosts the photoreceptive pineal organ is considered by many to be essential to the generation, synchronisation and maintenance of circadian and seasonal rhythms. This would be primarily achieved via the action of melatonin although direct evidence is still lacking in fish. In salmonids the production of pineal melatonin is regulated directly by light and levels are continually elevated under constant darkness. In non salmonid teleosts the rhythmic high at night/ low during day melatonin levels persists endogenously under constant conditions and is hypothesised to be governed by light and intra- pineal clocks. The aims of the present in vitro and in vivo trials were to determine if circadian clocks and Aanat2 expression, the rate limiting enzyme for melatonin IV production, are present in salmon, test the ability of the pineal to independently re-entrain itself to a different photoperiod and establish whether the candidate clock genes and Aanat2 expression can be sustained under un-entrained conditions. Expression of clock genes was first studied in vitro with pineal organs exposed to either 12L:12D photoperiod, reversed 12D:12L photoperiod and 24D. Clock gene expression was also determined in vivo, in fish exposed to 12L:12D. Results were then contrasted with an in vitro (12L:12D) investigation in the European seabass, a species displaying endogenous melatonin synthesis. Results revealed no rhythmic clock gene (Clock, per1 and per2) expression in isolated salmon pineals in culture under any of the culture conditions. In the seabass, Clock and Per1 did not also display circadian expression in vitro. However rhythmic expression of Cry2 and Per1 was observed in vivo in the salmon pineal. This suggested some degree of extra-pineal regulation of clocks in the Atlantic salmon. In terms of Aanat2 no rhythmic expression was observed in the Atlantic salmon under any experimental conditions while rhythmic expression of Aanat2 mRNA was observed in seabass pineals. This is consistent with the hypothesis that in salmonids AANAT2 is regulated directly at the protein level by light while in other teleosts, such as seabass, AANAT2 is also regulated by clocks at a transcriptional level.
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Chapman, Brent Maynard. „The effects of photoperiod, pinching, and plant growth regulators on growth and flowering of Abutilon x hybridum“. Thesis, Virginia Polytechnic Institute and State University, 1987. http://hdl.handle.net/10919/101174.
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Abutilon x hybridum, flowering maple has the potential to become a profitable floriculture crop. However, growing guidelines need to be developed in order to produce a proportional, well-branched, and uniform-flowering pot plant. The objective of this research project was to form a foundation on which a commercial growing program could be developed.
Growing flowering maple under short-day conditions (SD) significantly reduced plant height. Plants grown under SD were nearly half as tall as those plants grown under long-day conditions (LD). Also, plants grown under SD flowered an average of 5 days earlier than those plants grown under LD. Ancymidol at 50 ppm was successful in the reducing the height of nonpinched plants.
Chemical pinching agents, including mefluidide and Atrinal, induced more lateral branch development and increased flower production of both seed-grown and cutting-grown plants. As the concentration of mefluidide and Atrinal increased, so did the number of flowers that were produced. Atrinal at 1000 ppm appeared to be the treatment of choice for producing the most aesthetic and marketable flowering maple plant.
A foliar application of GA4+7 at 100 ppm was successful in elongating peduncles without producing a disproportional increase in plant height. The peduncles of both seed-grown and cutting-grown plants receiving 100 ppm GA4+7 were nearly twice as long as the controls. Thus, GA could be used to improve the flower display of Abutilon.M.S.
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Ikeda, Kento. „Role of the clock gene period in the circadian rhythm and photoperiodism of the silkmoth Bombyx mori“. Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/264645.
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McAllan, B. M. „The regulation of seasonal reproductive cycles in "Antechinus" : photoperiodic and pineal correlates /“. Title page, contents and abstract only, 1987. http://web4.library.adelaide.edu.au/theses/09SM/09smm114.pdf.
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Sprague, Matthew. „Environmental influences on the physiological and behavioural growth responses in salmonids : with reference to the growth-dip phenomenon“. Thesis, University of Stirling, 2006. http://hdl.handle.net/1893/212.
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Photoperiod manipulations are widely used throughout the Atlantic salmon (Salmo salar) farming industry as a means of producing a product of uniform quality all-year round. However, farmers still remain sceptical over their effectiveness to regulate growth and maturation during the on-growing stage. Furthermore, reports of a characteristic growth-dip following light exposure suggest that light may negatively affect the physiological performance of fish in the short-term. Thus, this thesis investigates the effects of light characteristics (spectral quality, intensity and photoperiod) on growth and maturation of salmonid fish and addresses some of the uncertainties surrounding photoperiod use currently reported within the industry. Rainbow trout (Oncorhynchus mykiss) are seemingly an ideal model species for examining photoperiod effects on growth. Consequently, the application of constant light exposure (LL) at two different intensities (28W and 16W) during two different thermal conditions (summer and winter) was examined on individually tagged fish. Feed intake and growth appeared to be related to the ambient water temperature and did not appear to be affected by intensity or photoperiod, although the onset of constant light did appear to initially affect growth rate. This may indicate that LL has a limiting effect on the growth of trout or that the prevailing water temperature at which light is applied may override the photoperiodic effect. Furthermore, the lack of enhanced growth in trout exposed to LL, unlike that demonstrated for other salmonids, suggest that there may be a species-specific response to environmental variables. Thus, questions regarding photoperiod effects should be limited to the species in question. The main source of variation in results observed under photoperiod manipulations stems from the salmon industry. Atlantic salmon post-smolts were reared in seawater tanks and either maintained under a natural photoperiod (NP) or exposed to a simulated natural photoperiod (SNP), constant light superimposed on the natural light (NPLL) or constant light only (LL). Artificial light onset, irrespective of photoperiod, resulted in an apparent trend for a reduced appetite lasting up to 60 days. Furthermore, the onset of constant light resulted in a significant chronic elevation of plasma cortisol levels and changes to growth and thyroid hormone levels, providing direct evidence that constant light exposure induces stress. In addition, fish exposed to SNP failed to exhibit a stress response despite a low feed intake. However, differences in the plasma melatonin levels during twilight times, as compared to NP, suggest that gradual changes in the natural light intensity throughout the day, particularly around dawn and dusk, may be important for synchronizing daily events. No differences in growth were observed between the NP and NPLL regimes, although fish reared in an enclosed regime (SNP and LL) exhibited a significantly lower weight gain than fish in an open environment (NP and NPLL). This further highlights the impact that the rearing environment has on the growth performances of fish and the need for commercially run trials. Advances in lighting technologies and a greater understanding of how light is transformed through the water column have focussed research on the spectral sensitivity of fish. Therefore the lighting efficiency of novel blue narrow bandwidth LED lighting units through the water column and their effects on growth and maturation performances of salmon reared in commercial production cages were compared against the standard metal halide units currently utilized throughout the industry. LL application, irrespective of intensity or spectrum, reduced the numbers of fish maturing as compared to fish reared under a natural photoperiod. However, this was greatest under the standard metal halide units reflecting a greater light penetration and perception as determined by plasma melatonin levels. The metal halide groups exhibited the greatest relative weight gain over the trial period as compared to control fish. No evidence was observed for a growth-dip under metal halide light, although blue lit treatments exhibited an initial significant reduction in food consumption, suggesting a possible welfare issue. Nevertheless, the prototype blue LED units showed possible potential for commercial application by penetrating the water depth at half the distance of the metal halide units for only one eighth the power and one fifth the brightness. However, further tests of these prototype spectral units are required to examine the potential welfare and physiological growth and reproductive effects. These studies have shown that the efficacy of artificial light regimes is largely dependent upon the effectiveness of the light source through the underwater environment and its perception by fish, providing a sufficient intensity is emitted exceeding the physiological threshold level for the species cultured. Moreover, whilst the onset of artificial light may elicit a stress response and demonstrate a trend for a suppression of appetite for salmon reared in experimental tanks, no compelling evidence for a suppression of appetite or growth was found under normal commercial cage conditions. This suggests that the growth-dip observed within the industry may in part be a combination of a physiological response to the onset of light further exaggerated by the farmer’s perception and altered judgement in feeding. In addition, the results obtained from this study have helped to standardize the use of light regimes within the industry. Nevertheless, further studies are necessary to fully elucidate the underlying mechanisms which may govern growth and maturation in fish following the onset of light exposure.
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De, Jager Pieter Herodes. „Effect of photoperiod on sexual development, growth and production of quail (coturnix coturnix japonica)“. Thesis, Port Elizabeth Technikon, 2003. http://hdl.handle.net/10948/227.
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The aim of the study was to determine the effect of photoperiod on production of quail in South Africa. Day old chicks were randomly divided into two groups. One group (n=74) received continuous light (LL) while the other group (n=77) received 13 h of light (LD) per day. Traits measured were sexual characteristics (age at sexual maturity and testis weight, egg production and egg weight) and growth (body weight) development in males and females respectively. Significant differences in growth existed between sexes; females were 171.44±17.15 and 182.91±17.75 g compared to the 151.77±13.20 and 155.00±16.86 g for males in both LL and LD groups respectively. Both males and females in the LL group initially outperformed the LD group in growth rate but, by day 72 the LD group had compensated and were 4.6% heavier than the LL group. A similar trend was observed for sexual maturity between the LL and LD groups. However, photoperiod did significantly influence initial egg production in favour of the LL group. The LL group had 80% of the females in production by day 44 compared to the 60% of the LD group. The initial egg weight of the LL group was 14 % higher than those of the LD group. Quail subjected to continuous light attained earlier sexual maturity and production with lower final weight compared to quail subjected to an intermediate photoperiod. The application of a longer photoperiod would therefore favour an egg production system.
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Rondon, Josimara Nolasco. „Autoecologia de Bauhinia holophylla Steud. (Leguminosae-Caesalpiniolideae), na Reserva Biologica e Estação Experimental de Moji Guaçu, SP“. [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315250.
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Orientador: Lilian Beatriz Penteado ZaidanTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Uma população de Bauhinia holophylla Steud. localizada no cerrado sensu stricto da Reserva Biológica e Estação Experimental de Moji Guaçu (RBEE de Moji Guaçu) foi estudada quanto a sua fenologia reprodutiva, expectativa de vida foliar, predação de sementes, germinação, fotoperíodo e regeneração natural. O padrão de distribuição da população adulta e jovem de B. holophylla, na área estudada é do tipo agregado. A população jovem e adulta de B. holophylla na RBEE de Moji Guaçu pode ser considerada juvenil, concentrando a maioria dos indivíduos nas primeiras classes de crescimento em altura e diâmetro. A expectativa média de vida da folha no primeiro ano de estudo foi de 8,2 meses, enquanto no segundo ano foi de 7,6 meses. A produtividade primária da fração folhas de 21 plantas de B. holophylla amostradas foi mais intensa nos meses de julho a agosto, durante o período de estudo (dois anos), portanto, durante a estação seca. A fração flores e botões esteve associada ao período da estação chuvosa, com produção maior nos meses de novembro a janeiro. A mortalidade foliar coincidiu com o período de menor precipitação na estação seca. A queda total das coortes foliares ocorreu ao mesmo tempo, na estação seca, estando a presença da folha durante a estação chuvosa, relacionada à produção de fotoassimilados para a planta, que serão usados na reprodução e/ou armazenados como amido no xilopódio. Plantas de B. holophylla apresentaram maior altura e diâmetro caulinar, e maior número de folhas nos tratamentos fotoperiódicos de 16 e 20 horas que nos tratamentos de 8 e 12 horas. A predação de sementes é uma das principais causas da inexistência de plântulas no cerrado. Contudo, a regeneração natural não está comprometida devido à presença do xilopódio. As sementes de B. holophylla são neutras à luz para a germinação e germinam em uma faixa ampla de temperatura. A permanência efêmera de sementes de B. holophylla no banco de sementes do solo de cerrado deve-se muito provavelmente à neutralidade à luz e velocidade de germinação alta em temperaturas na faixa de 25ºC a 30ºC. Práticas de manejo na RBEE de Moji Guaçu a partir do controle de larvas e de insetos predadores são fundamentais para aumentar a produção de sementes sadias de B. holophylla. Investigações sobre auto-ecologia, serapilheira, fenologia reprodutiva e dinâmica populacional devem ser propostas para espécies do Cerrado. Essas investigações podem auxiliar nos programas de recuperação de áreas remanescentes de cerrado e constituem modelos para estudos de populações de espécies vegetais do cerrado
Abstract: A population of Bauhinia holophylla located at a cerrado area in the Reserva Biológica e Estação Experimental de Moji Guaçu was studied in relation to reproductive phenology, leaf life span, seed predation, seed germination and aspects of plant regeneration. The distribution pattern of the juvenile and adult population is considered agregated. This population can be considered on the juvenile stage, due to, the greater number of plants concentrate in to the first size classes (height and diameter). The mean leaf life span was 8.2 months in the first year and 7.6 months in the second year. The primary productivity of leaves was more intense, during dry season (July-August). Leaf mortality with the period of the minor precipitation registered (dry season). Flower and bud production was associated to the wet season. Leaf fall ocurred during dry season. The new leaves could garantee the production of photoassimilates that would be utilized by all plant organs in their metabolic activities and as a reserve carbohydrate stored in the xylopodium as starch. Higher stems and stem diameter and leaf number in plants were observed in the photoper iodic treatments of 16h and 20 hours (long days) than in plants maintened in 8h and 12 hours (short days). Seeds predation is the principal cause of inexistence of seedlings in that the cerrado area. However, plant regeneration is provided by xylopodium. The seed is neutral to light for germination at the temperature range of 20ºC to 35ºC. Germination speed germination at 25ºC in the dark was higher than under light. The ephemeral permanence of the seeds of B. holophylla in the cerrado soil seed bank, is probably due to neutrality of light and their fast germination at 25ºC-30ºC. Thus, management polices at the Reserva Biológica e Estação Experimental de Moji Guaçu to decrease larvae and predator insects are fundamental to enlarge the production of healthy seeds of B. holophylla. Investigation on auto-ecology, litter and reproductive phenology, may give important information about of species occurring in the Cerrado. Investigations can support recuperation programmes of remnant cerrado areas and constitute model to study populations of plants species the Cerrado
Doutorado
Biologia Vegetal
Doutor em Biologia Vegetal
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Chew, Yin Hoon. „Multi-scale whole-plant model of Arabidopsis growth to flowering“. Thesis, University of Edinburgh, 2013. http://hdl.handle.net/1842/8008.
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In this study, theoretical and experimental approaches were combined, using Arabidopsis as the studied species. The multi-scale model incorporates the following, existing sub-models: a phenology model that can predict the flowering time of plants grown in the field, a gene circuit of the circadian clock network that regulates flowering through the photoperiod pathway, a process-based model describing carbon assimilation and resource partitioning, and a functional-structural module that determines shoot structure for light interception and root growth. First, the phenology model was examined on its ability to predict the flowering time of field plantings at different sites and seasons in light of the specific meteorological conditions that pertained. This analysis suggested that the synchrony of temperature and light cycles is important in promoting floral initiation. New features were incorporated into the phenology model that improved its predictive accuracy across seasons. Using both lab and field data, this study has revealed an important seasonal effect of night temperatures on flowering time. Further model adjustments to describe phytochrome (phy) mutants supported the findings and implicated phyB in the temporal gating of temperature-induced flowering. The improved phenology model was next linked to the clock gene circuit model. Simulation of clock mutants with different free-running periods highlighted the complex mechanism associated with daylength responses for the induction of flowering. Finally, the carbon assimilation and functional-structural growth modules were integrated to form the multi-component, whole-plant model. The integrated model was successfully validated with experimental data from a few genotypes grown in the laboratory. In conclusion, the model has the ability to predict the flowering time, leaf biomass and ecosystem exchange of plants grown under conditions of varying light intensity, temperature, CO2 level and photoperiod, though extensions of some model components to incorporate more biological details would be relevant. Nevertheless, this meso-scale model creates obvious application routes from molecular and cellular biology to crop improvement and biosphere management. It could provide a framework for whole-organism modelling to help address global issues such as food security and the energy crisis.
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Konagaya, Tatsuro. „Ecophysiological study on the alternative life cycles of males in the Japanese common grass yellow Eurema mandarina“. Kyoto University, 2018. http://hdl.handle.net/2433/232289.
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Matsuda, Naoki. „Physiological study on the transgenerational timing mechanism in an aphid“. Kyoto University, 2020. http://hdl.handle.net/2433/253117.
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Gillanders, S. W. „A comparison of the circadian control of locomotor activity and photoperiodism in Drosophila melanogaster, wild-type and period mutants“. Thesis, University of Edinburgh, 1993. http://hdl.handle.net/1842/14915.
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Mutant Drosophila melanogaster flies with drastically altered free-running periods of locomotor activity (period mutants) were used to investigate the relationship between circadian control of overt rhythm production and photoperiodic time measurement. Comparison of the free-running rhythms of wild-type, short period and long period mutants revealed that alterations in this periodicity resulted from a decrease in the active phase (short period mutant) or an increase in the inactive phase (long period mutant) when compared with wild-type. Light phase response curves were determined for light pulses of different durations which suggested that the mutants also had an altered sensitivity to light. Comparisons of the photoperiodic response of these strains revealed a remarkable similarity from which it was concluded that the period locus is not essential for PPTM but may be involved in a peripheral role. The light PRC data were used to model the CDL of the photoperiodic response in the wild-type, short period and long period mutants and the modelled results did not agree with the experimental ones. A feedback model for photoperiodic time measurement involving two oscillations, a pacemaker controlling overt rhythmicity and weakly coupled to a slave involved in PPTM was shown to replicate the experimental results. In conclusion, although the locomotor activity rhythm and the photoperiodic time measuring system are both governed by circadian oscillators, they are believed to be, at best, weakly coupled together so that the oscillator controlling locomotor activity may play a role in photoperiodism.
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Johnson, Kerry. „Photoperiod induction, Gibberellic acid, mulch and row cover effects on fresh cut flower production of three Rudbeckia hirta L. cultivars“. Diss., Mississippi State : Mississippi State University, 2006. http://sun.library.msstate.edu/ETD-db/ETD-browse/browse.
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Al-Khamees, Sami A. „Photoperiod effects on circadian rhythms and puberty onset in African catfish Clarias gariepinus“. Thesis, University of Stirling, 2009. http://hdl.handle.net/1893/1819.
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Photoperiod manipulation is routinely used in the aquaculture industry with the aim to enhance growth by manipulating the timing of reproduction in several commercially important temperate fish species. However, there are clear gaps in our understanding of how photoperiod is perceived by the circadian axis and transmitted to the brain to alter reproduction. Furthermore, due to the wide range of environments inhabited by fish, it is unlikely that one single organization exists. It is therefore believed that comparative studies of temperate species “models” with tropical species such as the African catfish (Clarias gariepinus) that adapted to different environments characterized by weaker light signals can help in such an aim. A number of studies were therefore performed in this PhD project to expand our knowledge on circadian biology and environmental physiological effects in African catfish. The first aim was to characterize the circadian melatonin system in this species (chapter 3). Results clearly showed that the control of melatonin production by the pineal gland was very different in the African catfish as compared to temperate species such as salmon and trout. Indeed, melatonin production appeared to mainly depend on light stimuli perceived by the eyes as opposed to salmonids where light directly perceived by the pineal gland regulates its own melatonin production within photoreceptors. The main evidence was obtained in ophthalmectomised fish that were unable to synthesize and release melatonin into the blood circulation during the dark period. This was the first time that such a decentralized organisation, similar in a way to the mammalian system, was found in any teleost species. In vitro results also supported such findings as African catfish pineal glands in isolation were not able to normally produce melatonin at night as usually seen in all other fish species studied so far. This indirectly suggested that pineal gland photo-sensitivity might be different in this tropical species. Further studies were performed to better determine the amount of light that can be perceived by the African catfish pineal gland depending on light transmittance though the skull (where the pineal gland is located). Surprisingly, it appeared that catfish cranium act as a stronger light filter than in other species resulting in lower light irradiance of the pineal gland. This could explain, although it still needs to be further confirmed, why African catfish photic control of melatonin produced by the pineal would have evolved differently than in temperate species. The work then focused on better characterizing diel melatonin production and endogenous entrainment through exposure to continuous photic regimes (continuous light, LL or darkness, DD) (chapter 4). Daily melatonin profiles of fish exposed to 12L:12D photoperiod (routinely used in indoor systems) confirmed low melatonin production at day (<10 pg/ml) and increase at night (50 pg/ml) as reported in most vertebrate species studied to date. Interestingly, results also showed that melatonin production or suppression can anticipate the change from night to day with basal melatonin levels observed 45 mins prior to the switch on of the light. These observations clearly suggest the involvement of a clock-controlled system of melatonin secretion that is capable of anticipating the next photophase period. Furthermore, when constant light (LL) was applied, day/night melatonin rhythms were abolished as expected due to the constant photic inhibition of AANAT activity (e.g. one of the enzyme responsible for the conversion of serotonin into melatonin). However when fish were exposed to constant darkness (DD), a strong endogenous melatonin rhythm (maintained for at least 4 days and 18 days in catfish and Nile tilapia respectively) was found, demonstrating once again the presence of robust circadian oscillators in this species. The next aim of the doctoral project was then to investigate circadian behaviour of catfish through locomotor activity studies (Chapter 5). African catfish is again a very interesting “model” due to its reported nocturnal activity rhythmicity as compared to most other teleosts species. Locomotor activity is considered as a very useful tool to elucidate the mechanisms of circadian organization in both invertebrates and vertebrates circadian. Results first confirmed the nocturnal activity rhythms in the species. Furthermore, clear circadian endogenous rhythms were observed under constant light (LL) or darkness (DD) during several days before losing rhythmicity. Interestingly, the activity levels varied depending on the stocking density. Finally, the last aim of this project was to test the effects of a range of photoperiodic manipulations on growth performances, sexual development and reproductive performances in African catfish reared from eggs to puberty. Results did not show any differences at the early sages (up to 90 days post hatching) in growth performances nor mortality (high) between control 12L:12D and LL treatments. In contrast, during the juvenile-adult period (from 120 to 360 DPH), significant growth effects were observed, as previously reported in other catfish species, with fish under LL displaying lower growth rate, food consumption and feed conversion efficiency in comparison to most other treatments (12:12, LL, 6:6, 6:18, 12-LL and LL-12) especially 12l:12D. However, no major effects of the photoperiodic treatments were observed with all fish recruited into puberty and developing gonads although differences in the timing of gametogenesis could be observed, especially a delay (circa 2 months) in females exposed to short daylength (6L:18D and 6L:6D). As for egg quality, egg diameter was the only parameter to differ between treatments (slightly larger in egg batch from LL treated females). Overall, none of the photoperiodic regime suppressed maturation in African catfish as opposed to some temperate species. The work carried out during this PhD project clearly advanced our understanding of circadian rhythmicity, light perception and effects of photoperiod on physiology in a tropical species. Future studies are now required to further characterise the circadian system and link it to evolutionary trends within vertebrates.
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Taylor, John Frank. „The effects of photoperiod manipulation on growth and reproduction in rainbow trout (Oncorhynchus mykiss)“. Thesis, University of Stirling, 2004. http://hdl.handle.net/1893/12323.
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Photoperiod is an important signal involved in the timing and co-ordination of many processes such as growth and reproduction in salmonids. Both growth and reproduction appear to be controlled by endogenous rhythms, which under natural conditions, is entrained by the seasonal changes in daylength, that is accurately reflected by the diel pattern of melatonin. This thesis investigates the influence of photoperiod on growth and reproduction in rainbow trout (Oncorhynchus mykiss), and examines the effect on plasma insulin-like growth factor-I (IGF-I). These studies aim to further our scientific understanding of the endocrine mechanisms controlling growth and reproduction and transfer this knowledge to commercial trout farming practices. Studies at fry (<5g) and fingerling (>25g) stages investigated the effect of exposure to constant long-days (LD18:6) and constant light (LL) on growth performance. In all experiments, there was a significant growth enhancing effect following exposure to extended light regimes relative to those under ambient or constant short-day photoperiods. Growth enhancement appeared to be caused through direct photo-stimulation or a phaseshift in an underlying endogenous rhythm dependent on the timing of photoperiod application. Measurement of plasma IGF-I accurately reflected growth rate during juvenile development. Furthermore, a clear autumnal increase in IGF-I was observed that was apparently up-regulated by long-day photoperiods. Additionally, it was shown that melatonin implants significantly reduced growth rate below that of unimplanted controls irrespective of photoperiod, but did not reduce circulating IGF-I levels suggesting that elevated melatonin levels masked the perception of daylength but did not act directly on the somatotropic axis to control growth. As size or one of its correlates (growth rate, energy balance or nutritional status) is regarded as an important determinant of the ability to undergo puberty, the interaction of natural (SNP) and advancing (ADV: long-short day) photoperiods with growth, IGF-I and reproduction was investigated in virgin female broodstock. Under SNP 63% of the population attained maturity while only 29% spawned in the ADV regime. Under SNP both size and growth rate in late spring/early summer appeared to determine whether an individual may initiate maturation. Conversely, under ADV, condition factor appeared to be a better predictor. A complete seasonal relationship between plasma IGF-I, daylength and temperature was demonstrated for the first time in rainbow trout under natural conditions, and provides direct evidence for the relationship between maturation and IGF-I. IGF-I levels showed a negative correlation with testosterone as fish initiated maturation. Furthermore, IGF-I levels accurately reflected growth rate prior to the initiation of vitellogenesis, suggesting that IGF-I may provide an endocrine signal between the somatotropic and reproductive axes that nutritional status, growth rate and/or size is sufficient to initiate maturation. In addition, maturing individuals under both photoperiods typically expressed higher circulating IGF-I levels than those that remained immature and may reflect a greater opportunity for IGF-I to act on the pituitary to stimulate gonadotropin production. In this sense, the observation of elevated levels for 3 months under SNP compared to only 1 month under ADV may reflect a reduction in the window of opportunity to initiate maturation under advancing photoperiods and hence explain the reduction in fish spawning. Finally, using a commercially available ELISA kit provided evidence of a possible detection method for measurement of a leptin-like peptide as absolute levels differed significantly between mature and immature fish. This provides preliminary evidence for a possible involvement of a leptin-like peptide in rainbow trout reproduction and energy homeostasis. Given the above evidence of a growth enhancing effect of photoperiod in covered systems, the transfer of constant light (LL) regimes to uncovered commercial trout farming practices was also examined. The application of LL regimes during either fry grow-out in tanks using floating lights or ongrowing to harvest size during winter in cages using submersible lights was investigated. In all trials conducted, the exposure to LL in autumn significantly enhanced winter growth rate and feeding efficiency by up to 30% and 25% respectively relative to those maintained under ambient light. Furthermore, the effect of light intensity was clearly shown to be an important requirement in successfully applying photoperiod regimes onto the ambient photoperiod in order to enhance commercial production in "uncovered systems". These studies clearly indicate the importance of photoperiod in influencing growth and reproduction in rainbow trout. Furthermore, the use of plasma IGF-I as an indicator of growth provides a practical tool for studying growth-photoperiod-reproduction interactions in this species. However, further studies are necessary to further our understanding of the endocrine pathways governing physiological mechanisms, especially growth and reproduction. Overall, this work has provided important information to improve both scientific understanding and commercial development although it is clear that substantial research is still required.
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Rutterford, Zoë Susan. „The barley circadian clock in relation to photoperiod response“. Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609468.
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Magiero, Emanuelle Cavazini. „Fisiologia da floração e atividade alelopática de Artemisia annua L. cultivar Artemis cultivada em clima subtropical úmido“. Universidade Tecnológica Federal do Paraná, 2009. http://repositorio.utfpr.edu.br/jspui/handle/1/237.
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A Artemisia annua L. (Asteraceae) é uma planta herbácea, nativa da Ásia e aclimatada no Brasil. As folhas apresentam até 1,4% do peso seco em artemisinina, sendo fonte abundante desta lactona sesquiterpênica que, conjuntamente aos seus derivados semi-sintéticos, apresentam ação efetiva contra as cepas resistentes das espécies de Plasmodium causadoras da malária. A artemisinina também está sendo testada com sucesso no tratamento de câncer e apresenta-se efetiva contra inúmeros parasitas que afetam a saúde humana e animal. Os objetivos deste trabalho foram determinar as épocas de plantio e colheita da cultivar Artemis de A. annua em Pato Branco-PR, como também, determinar em campo o fotoperíodo crítico e o número de ciclos fotoindutivos necessários para o florescimento desta cultivar. Um segundo experimento foi realizado com o objetivo de determinar o potencial alelopático desta espécie sobre sementes de alface e leiteiro. No experimento em campo, os tratamentos utilizados foram seis diferentes épocas de
plantio, sendo as 09 plantas centrais das parcelas colhidas para a determinação da
massa seca de folha e caules, relação folha/caule e teor de artemisinina na massa
seca de folhas quando 50% das plantas da parcela iniciaram a emissão dos botões
florais. O número de ciclos fotoindutivos encontrado para a cultivar Artemis foi de
aproximadamente 36 ciclos e o fotoperíodo crítico aproximado foi de 13 horas, que
ocorreu em 09/02/2007 em Pato Branco-PR. Esse resultado determinou que a época
de colheita para a cultivar Artemis, na região de Pato Branco-PR, deve ser na segunda quinzena do mês de março. O maior rendimento de biomassa nesse experimento ocorreu no plantio de 27/07/2007, 231 dias após o transplantio, mas recomenda-se tomar cuidado com as geadas que podem ocorrer até a primeira quinzena de setembro, sugerindo-se optar pelo plantio na segunda quinzena de setembro, período livre de geadas na região. Os maiores teores de artemisinina (em média 0,80% na matéria seca de folhas) foram obtidos nas épocas que mais
vegetaram a campo devido à maior produção de biomassa. Para o experimento de alelopatia, o extrato aquoso bruto foi preparado a partir de folhas frescas na proporção de 1 L de água destilada para 250 g do material. Foram utilizadas as seguintes concentrações do extrato aquoso: 100%, 75%, 50%, 25% e 0% (água destilada). Foram analisadas as variáveis germinabilidade, velocidade média de germinação, tempo médio de germinação, comprimento da radícula e massa seca
das plântulas. Extratos aquosos de A. annua apresentaram ação alelopática inibitória sobre a germinação de sementes e o desenvolvimento de plântulas de alface e leiteiro.The Artemisia annua L. (Asteraceae) is a herbaceous plant, native of Asia and acclimated in Brazil. The leaves are abundant source of artemisinin, a sesquiterpene lactone that, jointly to yours derived semi-synthetic, they present effectiveness action against the resistant stumps of the species of Plasmodium against malaria. The artemisinin is also being tested with success in the cancer treatment and against countless parasites that affect the human and animal health. The objectives of this work went to determine the planting times and crop of the cultivar Artemis of A. annua in Pato Branco-PR, as well as, to determine in field the critical photoperiod and the number of inductive photoperiod for the flowering of this cultivar. A second experiment was accomplished with the objective of determining the allellopathic effect of this species on lettuce seeds and wild poinsettia. In the experiment in field, the treatments were six different transplant times, being the 09 central plants of the portions picked for the determination of the mass dries of leaf and stems, relationship leaf/stems and artemisinin in the mass dries of leaves when 50% of the plants of the portion began the emission of the floral buttons. The number of cycles inductive found for the cultivar Artemis was of approximately 36 cycles and the critical photoperiod was approximately 13 hours, that happened in 09/02/2007 in Pato Branco-PR. This results determines that the crop time for cultivar Artemis, in Pato Branco-PR will be in the second fortnight of the month of March. The largest biomass income in that experiment happened in the planting of 27/07/2007, 231 days after the transplant, but it is recommended to take care with the frost that can happen until the first fortnight of September, suggesting choose for the transplant in the second fortnight of September, period free from frost in the area. The largest artemisinin tenor (0,88% in dry matter leaves) was obtained in the times that more they vegetated to field due to the largest biomass production. Besides this experiment, a second experiment was developed to determine the allellopathic effects of this species on lettuce seeds and wild poinsetia. The aqueous extract was prepared starting from fresh leaves in the proportion of 1 L of water distilled for 250 g of the material, after, the extract was filtrate and centrifuged, being used the sobrenadante. The following concentrations of the extract were used: 100%, 75%, 50%, 25% and 0% (distilled water). They were analyzed the variables germinability, germination velocity, germination time, length of the radicle and weight of the dry matter of the seedlings. Aqueous extracts of A. annua is allellopathic on the germination and development of lettuce and wild poinsettia seedlings.
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Doong, Tzyy-Jye. „Regulation of Gibberellin Metabolism by Environmental Factors in Arabidopsis Thaliana“. Columbus, Ohio : Ohio State University, 2002. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1034085989.
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Thesis (Ph. D.)--Ohio State University, 2002.Title from first page of PDF file. Document formatted into pages; contains xi, 148 p.; also contains graphics (some col.). Includes abstract and vita. Advisor: James Metzger, Dept. of Horticulture and Crop Science. Includes bibliographical references (p. 132-148).
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Covington, Michael Fulton. „ELF3 and the light resetting mechanism of the circadian clock in Arabidopsis thaliana /“. view abstract or download file of text, 2002. http://wwwlib.umi.com/cr/uoregon/fullcit?p3061940.
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Doyle, Alice. „Understanding maturity : insights into the mechanisms underpinning maturity in gadoids“. Thesis, University of Stirling, 2016. http://hdl.handle.net/1893/25265.
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Marked shifts in the life history traits of fish have been reported in many exploited fish stocks, with a particular trend towards decreasing size and age at maturity. Though other environmental and behavioural factors have been implicated, the key driver of these changes links to fishing pressure, through both the direct selective effects of fishing itself, and indirectly through the manipulation of important biological and environmental factors. Although reproduction itself has been well described in teleosts, the mechanisms of environmental and endogenous entrainment of maturation remain unclear and it was the principal aim of this thesis to improve current understanding of these systems in gadoids. Photoperiod has been identified as the strongest environmental cue for entraining seasonal behaviours, including seasonal reproduction. Over the last decade, several of the key drivers involved in the photoneuroendocrine cascade have been elucidated in mammals and birds, with the Eya3 pathway merging as an important mechanism for entraining maturation. However, little is yet known of their influence on maturation in fish. In the first study, the photoperiodic regulation of the Eya3-Tshβ-Dio2 cascade was analysed in Atlantic cod exposed to either continuous light (reproductive inhibition) or simulated natural photoperiod (reproductive stimulation) from July to December. Monthly expression was measured through QPCR, demonstrating a strong activation of pituitary Eya3 under declining photoperiod. As this coincided with the onset of secondary gametogenesis, these results suggest that Eya3 may play a stimulatory role in the photoneuroendocrine cascade of Atlantic cod. Although photoperiod represents the most reliable and noise free proximate signal to entrain the reproductive process, it is clear that a minimum growth and energetic state must be reached for maturation to progress. This directed the second line of study – a series of diet restriction trials on haddock and cod designed to investigate the influence of naturally occurring lipid levels in the diet on growth and reproduction, define the “critical window” in which fish assess their energetic state and how this is analysed before commencing secondary gametogenesis, and to assess whether Eya3 is regulated by the growth axis in cod. The results of these experiments indicate that overall size around the autumn equinox is the most accurate indicator of maturation commitment in cod. Additionally, Eya3 expression was elevated in maturing fish indicating a role for this marker in linking the energetic signal with entrainment of the reproductive axis. Finally, an analysis of the physiological and genetic stock structure of cod from the North Sea IVa stock region and westward into VIa was performed. The results of this analysis support previous genetic studies, indicating further fine-scale structuring of these stocks, reflective of the structure indicate by the differences in maturation strategies of the component populations. The results identified both environmental and harvest related pressures which may be driving the current stock structure. The results of these studies greatly improves our understanding of the key drivers and mechanisms regulating maturation in cod, highlighting new avenues for future research.
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Corrêa, Simone Toni Ruiz. „Adaptação do modelo LINTUL (Light Interception and Utilization) para estimação da produtividade potencial da cultura de soja“. Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-11022009-100352/.
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Em 2007 o complexo de soja foi responsável por 19 por cento do total das exportações do agronegócio brasileiro, gerando mais de U$11 bilhões em divisas. Projeções indicam que até 2020 o Brasil deverá produzir 105 milhões de toneladas de soja, ou 81 por cento a mais do que a atual produção. Apesar de incidir sobre a produtividade a maior expectativa de crescimento, espera-se que a expansão significativa em área ocorra nas regiões de baixa latitude dos Cerrados, e em especial, no estado do Mato Grosso. É fundamental a determinação de estratégias que considerem produção, dimensões econômicas e ambientais. O uso da modelagem agrícola é a única forma de explorar sistematicamente o potencial produtivo em escala global e predizer produtividades futuras em determinadas condições. A utilização de computadores e banco de dados torna-se uma ferramenta cada vez mais necessária, no sentido de disponibilizar rapidamente informações de qualidade a um custo relativamente inferior, quando em comparação a experimentos de campo. O modelo LINTUL, desenvolvido pela Universidade de Wageningen, foi extensamente utilizado para predizer a produtividade potencial de culturas baseado no uso eficiente da radiação solar, sob condições ótimas de desenvolvimento. A cultura de soja, porém, nunca foi testada por este modelo. Sendo assim, os objetivos deste trabalho foram: (i) adaptação, por intermédio da parametrização e da calibração do modelo LINTUL para estimação da produtividade potencial da cultura de soja; (ii) disponibilizar um algoritmo em Visual Basic (Microsoft), validando e replicando os procedimentos do LINTUL, tornando este modelo acessível a uma ampla gama de usuários. Para a parametrização do modelo, foram consideradas características específicas da cultura, como por exemplo, a sensibilidade ao fotoperíodo. Para calibração, foram usados dados de experimento de campo conduzido em Planaltina-DFe Sorriso- MT. Os parâmetros não avaliados no experimento foram extensivamente revisados com base na literatura. Os principais componentes do processo de crescimento e desenvolvimento da cultura foram analisados individualmente através de comparativos de respostas. Posteriormente, um estudo de adequação do modelo foi feito, com objetivo de viabilizar uma comparação de comportamentos, quando em diferentes situações. As variáveis climáticas usadas neste modelo foram temperatura máxima e mínima e radiação solar. A simulação dos estádios fenológicos é baseada na soma térmica. O modelo LINTUL-soja demonstrou excelente desempenho quanto à predição de tendências, com a variação dos valores dos parâmetros. Todavia, o modelo necessita de ajustes quanto a introdução de módulos, que contemplem o balanço de carboidratos. O uso de uma linguagem de programação aberta, como o Visual Basic, replicou a linguagem usada pelo modelo LINTUL com sucesso.In 2007 the soybean complex was responsible for more than 19% of the total Brazilian agribusiness exports, which corresponded to US$ 11 billion generated in currencies. According to projections, soybean production in Brazil will increase significantly till 2020, reaching 105 million tons, or an addition of 81 percent to the current crop season. That huge difference is expected to come from a significant increase in yield, while the increase in acreage should come from the low-latitude regions of Cerrados, mainly in Mato Grosso state. Thus, it becomes extremely important to set out strategies that consider production, economic and environmental dimensions. The usage of agricultural modeling is the only mean of systematically exploring the production potential on global scale and predict future yield in certain conditions. Computers and database application becomes an implement even more efficient in respect of turn quickly and quality information available with lower costs, when compared to the field experiments. The LINTUL model was developed by the Wageningen University, and extensively used to predict crop potential production based on radiation use efficiency concept, under optimal conditions of growth. The soybean crop has never been tested by this model. The goals of this research are: (i) to adapt - through parameterizing and calibration - the LINTUL model to estimate the potential production of soybean; (ii) to provide an algorithm in Visual Basic language, in order to validate the calculations and replicate the procedures executed by LINTUL, making this model accessible to a wide range of users. In order to parameterize the model, crop-specific characteristics were taken into account, such as the sensibility of soybean to photoperiod. In respect to calibration, it was used field data collected in Planaltina-DF and Sorriso-MT. The mainly components of the growth and development process were analyzed based on response comparatives. Afterward, it was done a study of adequacy of the model, in order to enable a comparison of behaviors, when in different situations. The climatic variables used in this model were maximum and minimum temperature and solar radiation. The simulation of phenological stages was based on thermal unit concept. The LINTUL-soybean model has demonstrated an excellent performance to predict trends, with the change in the parameters values. However, the model needs to be adjusted in respect to an introduction module to calculate the carbohydrates balance into the canopy. The use of an open programming language, such as Visual Basic, replied the language used by LINTUL model successfully.
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Weil, Zachary M. „Social And Temporal Determinants Of Brain, Behavior And Immune Function“. The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1219085420.
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FANTINI, ELIO. „Genetic selection for flowering time traits during speciation of tomato“. Doctoral thesis, Università degli Studi di Verona, 2010. http://hdl.handle.net/11562/343262.
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SINTESI
Negli ultimi dieci anni, un network di informazioni sulla famiglia delle Solanacee è stato creato all’interno progetto internazionale SOL (International Solanaceae Genome Project), il cui scopo finale è quello di spiegare come un insieme comune di geni/proteine può portare a una vasta gamma di organismi morfologicamente e ecologicamente distinti come quelli della famiglia delle Solanacee. Questo taxon comprende più di 3000 specie, molte delle quali si sviluppano nella regione andina- amazzonica dell’America meridionale. Il loro habitat varia enormemente, dalle foreste pluviali ai deserti all’alta montagna. Inoltre, quella delle Solanacee è la terza famiglia economicamente più importante tra quelle in cui sono presenti specie coltivate, superata solo dai cereali (come riso, mais e grano) e dai legumi (come la soia), e la più importante tra le colture orticole. Essa comprende, tra gli altri, pomodoro, patata, melanzana, peperone, petunia e tabacco.
Tra le solanacee, il pomodoro è stato scelto come riferimento, avendo un genoma diploide relativamente piccolo (950 Mb) per il quale sono disponibili linee pure omozigoti, oltre a un’avanzata mappa fisica basata su BAC utile per iniziare il sequenziamento. Esso offre inoltre il vantaggio di un ciclo vitale breve, della presenza di efficaci tecnologie di trasformazione, e della disponibilità di risorse genetiche e genomiche.
In questo progetto, abbiamo unito strategie di genomica strutturale e funzionale, al fine di studiare nel pomodoro uno dei caratteri più importanti in termini di fitness e di adattamento: la fioritura. Abbiamo concentrato i nostri sforzi nello studio dei geni coinvolti nella via regolativa fotoperiodica, giacché alcune specie selvatiche di pomodoro, che crescono tra 0 e -25 gradi di latitudine e tra 0 e 3700 m di altitudine, mostrano risposte fotoperiodiche diverse. Questi geni sono membri di tre famiglie geniche, i cui ortologhi in Arabidopsis e riso svolgono un ruolo chiave nella regolazione della fioritura in dipendenza della lunghezza del giorno. Esse sono la famiglia genica dei TCOL (tomato CONSTANS-like), quella dei Criptocromi e quella di GIGANTEA.
Nel capitolo 2 vengono fornite informazioni strutturali sull'organizzazione e sull'espressione di queste famiglie geniche nel pomodoro coltivato, e si analizza la microsintenia con Arabidopsis. Sono stati inoltre identificati quattro nuovi COL e un nuovo GIGANTEA.
Nel capitolo 3 è stata analizzata la diversificazione a livello di sequenza delle tre famiglie di geni durante la speciazione nel clade pomodoro, utilizzando sia approcci di sequenziamento che analisi dei profili di espressione. In particolare, abbiamo osservato un alto grado di selezione diversificante per TCOL3, che presenta anche un cline geografico nella frequenza delle mutazioni sinonime, suggerendo un ruolo di questo gene nell’adattamento alle basse latitudini.
Infine, nel capitolo 4, vengono usati quattro approcci di genetica inversa (VIGS, RNAi, TILLING e overespressione) per una caratterizzazione funzionale della famiglia genica dei Criptocromi di pomodoro. Nuovi mutanti cry1b- e cry2-, piante RNAi di CRY1b e CRY2 e overespressori di CRY1a sono descritti per la prima volta e nuovi fenotipi di sviluppo e fioritura sono attribuiti ai vari geni.In the last decade, a coordinated network of knowledge about the Solanaceae family has been created by the International Solanaceae Genome Project (SOL), whose final purpose is to explain how a common set of genes/proteins can result in a wide range of morphologically and ecologically distinct organisms like those in the Solanaceae family. This taxon includes more than 3000 species many of which evolved in the Andean/Amazonian regions of South America. Their habitats vary dramatically, from rain forests to deserts to high mountains. Moreover, the Solanaceae is the third most valuable crop family exceeded only by the grasses (e.g. rice, maize, wheat) and legumes (e.g. soybean), and the most valuable in terms of vegetable crops. It includes, among others, tomato, potato, eggplant, pepper, petunia, tobacco. Among Solanaceae, tomato was selected as a reference since it provides the smallest diploid genome (950 Mb) for which homozygous inbreds are available, as well as an advanced BAC-based physical map to start the sequencing. It also offers the vantage of short generation time, routine transformation technology, and availability of rich genetic and genomic resources. In this project, we merged structural and functional genomic approaches, in order to study in tomato one of the most important characters in terms of fitness and adaptation: flowering. We have focused our efforts in the study of genes involved in the photoperiodic regulatory pathway, since some wild tomato species, that growth between 0 and -25 degrees of latitude and between 0 and 3700 m of elevation, show different photoperiodic responses. These genes are members of three gene families, whose orthologs in Arabidopsis and rice play a key role in the regulation of flowering in dependence of day length. They are the TCOL (tomato CONSTANS-like), the CRYPTOCHROME and the GIGANTEA gene families. In chapter 2 we provide structural information on the organization and expression of the CRY, COL and GI gene families in tomato and we analyse the microsynteny with Arabidopsis. We also identify four new COL and one new GI gene. In chapter 3 we investigate the sequence diversification of the three gene families during speciation in the tomato clade, using both sequencing and expression profiling approaches. In particular, we observed a high degree of diversifying selection for TCOL3, that presents also a geographical cline in the frequency of synonymous mutations, suggesting a role of this gene in adaptation to low latitudes. Finally, in chapter 4, we use four reverse genetic approaches (VIGS, RNAi, TILLING and overexpression) for a functional characterization of the tomato Cryptochrome gene family. Novel cry1b- and cry2- mutants and RNAi plants and CRY1a overexpressors are described for the first time and novel developmental and flowering phenotypes are attributed to the various genes.
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Hoffman, Christopher John. „THE EFFECT OF PHOTOPERIOD AND TEMPERATURE UPON ADULT ECLOSION OF THE SWEETPOTATO WHITEFLY, BEMISIA TABACI (GENNADIUS)“. Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275371.
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Martinez, Chavez Carlos Cristian. „Photic Entrainment and onset of puberty in Nile tilapia Oreochromis niloticus niloticus“. Thesis, University of Stirling, 2008. http://hdl.handle.net/1893/354.
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Despite teleosts being the largest and most diverse group of vertebrates, fish models currently used to study photoperiodic effects on fish physiology have been limited to a few species, most of which are temperate seasonal breeders. The overall aim of this work was to expand our knowledge on circadian biology and environmental physiological effects in Nile tilapia (Oreochromis niloticus niloticus), a continuous breeding species of tropical-subtropical origin. The circadian light axis of Nile tilapia is described with regards to melatonin production. Circadian melatonin profiles of fish under 12L:12D photoperiods were observed to be low at day and high at night, suggesting melatonin to be an entraining signal as observed in all other vertebrates. When constant light (LL) was used, such day and night fluctuations where abolished. However when fish where exposed to constant darkness (DD) a strong robust endogenous melatonin rhythm was found, suggesting the presence of circadian oscillators in this species. Importantly, this endogenous rhythm was observed to be maintained for at least three weeks under darkness and proved to be circadian in nature. Moreover, although the melatonin system was able to produce day and night melatonin rhythms when exposed to a different (6L:6D) photocycle, the oscillator appeared to not be entrainable to such a short photo cycle when exposed to DD, as melatonin levels remained high. When comparing the circadian organization of different teleost species including Nile tilapia, preliminary studies showed at least three divergent circadian light organizations in teleosts. Nile tilapia was characterised by a pineal gland far less sensitive than in other fish species as demonstrated through in vitro studies. Furthermore, pineal melatonin production was clearly dependent on the light perceived by the eyes as ophthalmectomy resulted in basal plasma melatonin levels during the dark period. These findings are the first to be reported in a teleost and could be comparable to the circadian light organization of higher vertebrates such as mammals. The onset of puberty of Nile tilapia was studied with regards to the newly discovered Kiss1/GPR54 system. Such a system has recently been discovered in mammals and found to be the primary switch of the brain-pituitary-gonadal (BPG) axis. The results of this study not only suggest a link between the Kiss1/GPR54 system and the onset of III puberty in this tropical batch spawning teleost, that would be a highly conserved feature across vertebrates, but also that the transcriptional mechanisms regulating GPR54 expression could be directly or indirectly influenced by light. Finally, a study was conducted on the effects of different intensities of continuous light (LL) on the growth and sexual development of Nile tilapia up to first maturation. The results showed a significant growth response of fish in all LL treatments compared to control fish. Importantly, this confirmed that LL enhances growth in this species and suggests that it is the light regime more than the intensity which is having an effect. This work thus provides important basic knowledge of the light entrainment pathway and circadian melatonin rhythms in Nile tilapia. Of special importance is the discovery of a strong endogenous melatonin oscillator and a novel circadian organization in fish which would seem to be homologous to that observed in higher vertebrates. Moreover, this work provides evidence that the newly discovered Kiss1/GPR54 system has a similar role in fish as has been found in mammals and that such a system could be directly or indirectly regulated by light. If so, Nile tilapia and other fish species could become important models in the chronobiology and reproduction fields. Finally, this work not only increases our basic and applied knowledge of this species, but also broadens our understanding of the circadian light axis in teleosts and its mediatory effects on reproduction.
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Davie, Andrew. „Effects of photoperiod manipulation on growth and reproduction in Atlantic cod (Gadus morhua L.)“. Thesis, University of Stirling, 2005. http://hdl.handle.net/1893/109.
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Sexual maturation during commercial culture of Atlantic cod (Gadus morhua L.) represents a significant production bottleneck restricting the profitability of the industry. Such problems in other species have traditionally been addressed by artificial manipulation of photoperiod cycles, however little research exists in this field in cod. This thesis therefore investigates the interactions between artificial photoperiod manipulation, sexual maturation and somatic growth in this species. In the first experiment, populations of Atlantic cod (hatched, spring 1999) were maintained on either a simulated natural photoperiod (SNP) or continuous illumination (LL) from approximately 15 months post hatch (MPH) (July 2000) in an enclosed tank system. Growth performance was recorded monthly along with observations of reproductive activity over the subsequent 2 years (up to July 2002). At both 2 and 3 years of age the entire population raised under SNP matured and spawned, during which time mean weight reduced by 13% and 24% respectively. No spawning individuals were recorded at 2 years of age in the LL population and only 18% were observed to spawn at 3. However, observations of both changes in gonadal morphology (observed via ultrasound scanning) and a suppression in growth rate at 2 years of age in the LL population alluded to a maturation “dummy run” regulated by an endogenous clock. Despite this phenomenon, the LL treatment realised a 39% and 43% improvement in wet weight following 1 and 2 years of exposure to LL respectively. When the diel cycle of plasma melatonin was compared between the treatments in February 2001 (23MPH) the SNP population displayed an A-profile diel rhythm ranging between 20 and 50 pg/ml while the LL treatment did not display any rhythm. In the second experiment of this work, two populations of cod (hatched, spring 2001) were reared in commercial open cage systems, one of which experienced continuous additional artificial illumination between July 2002 (15MPH) and October 2003 (30MPH) provided by four, 400W submerged lighting units. Growth and maturation were assessed in both populations throughout. In March 2003 (24MPH) it was apparent that spawning individuals were present in both the SNP and LL populations though a significantly lower number of spawning individuals in the LL treatment suggested that the peak in spawning activity was delayed by about 1 to 2 months. With both populations apparently maturing at 2 years of age, there was no significant difference in weight between the populations at the end of the trial. In comparison to experiments I and IV of this work, these results would suggest that in comparison to salmonids for example, Atlantic cod appear to have a heightened sensitivity to light allowing individuals to differentiate the ambient photoperiod signal from the application of continuous artificial light. In the third experiment, 6 populations of approximately 20 tagged individuals (hatched spring 1999) were maintained, from December 2000 to July 2002, under either SNP, LL or one of four, out of season “square wave” photoperiod regimes (repeating cycles with a 12 month period, consisting of a 6 month window of LL followed by six months of short day lengths [SD, 7L:17D] which had been staggered to start over a six month period). Each individual was monitored monthly for maturation status. Out of season “square wave” photoperiods were demonstrated to successfully entrain maturation and hence significantly alter the spawning profiles in these populations. Application of LL from December 2000 failed to inhibit maturation in the spring of 2001 and, in fact, advanced the spawning season by 1 month while those that experienced SD from the same date showed significant extension of the subsequent spawning season. Interestingly, the males maintained on LL throughout the experiment matured both in the spring of 2001 and one year later in the spring of 2002 while females under the same treatment only matured and spawned in 2001. In the fourth experiment, a total of 830 tagged individuals were raised either under SNP or one of 7 photoperiod treatments, consisting of 5 groups transferred from SNP to LL at 3 monthly intervals between 6 and 18 MPH where they remained and a further two groups maintained on LL from 6 to 15MPH and 6 to 21 MPH respectively before being returned to SNP. Both the gonadic and somatic axes were monitored at the physiological and endocrinological level at three monthly intervals from 6 to 27 MPH. The results demonstrated that it is the falling autumnal photoperiod signal after the summer solstice, more specifically after October, that is responsible for recruiting individuals to enter the sexual maturation cycle. Furthermore, in all treatments where this signal was masked i.e. those which experienced LL starting at or prior to 15MPH, except for some restricted spermatogenic activity in the males testis observed at 27MPH, there was no significant reproductive activity and growth was improved by up to 60% at 27 MPH. While providing evidence for direct photic stimulation of somatic growth, the growth results were also correlated with the measurement of plasma IGF-I and demonstrated its potential as a tool to assess growth rates in the species. Plasma melatonin measured at 15MPH, as in experiment I, was suppressed in all populations which were under LL photoperiods. By identifying the photoperiod “window of opportunity” which recruits individuals into the sexual maturation cycle, this work was able to conclude that the application of LL from the summer solstice prior to maturation is the most efficient photoperiod strategy to be adopted by the aquaculture industry to realise maximum growth potential from their cultured stocks.
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Earl, Colin R. „The regulation of the timing of melatonin secretion in the sheep“. Title page, summary and table of contents only, 1989. http://web4.library.adelaide.edu.au/theses/09PH/09phe12.pdf.
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Includes bibliographical references (leaves 166-195) Addresses the nature of the central mechanisms involved in the regulation of the circadian pattern of secretion of the pineal hormone melatonin in the highly seasonal Suffolk breed of sheep. Provides new information on the behaviour of the onset and offset of melatonin secretion under different photoperiodic conditions.
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Lara, Márcio André Stefanelli. „Respostas morfofisiológicas de genótipos de Brachiaria spp. sob duas intensidades de desfolhação e modelagem da produção de forragem em função das variações estacionais da temperatura e fotoperíodo: adaptação do modelo CROPGRO“. Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-24052011-093743/.
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Para poder compreender e descrever o crescimento das plantas forrageiras é necessário ter conhecimento acerca de seus aspectos agronômicos e morfofisiológicos e suas respostas aos fatores edafoclimáticos. O desenvolvimento de ferramentas que viabilizem a racionalização dessas inter-relações é de grande importância para a comunidade científica, ao facilitar a explicação da dinâmica do crescimento, e para o pecuarista dando opções de planejamento e auxílio à tomada de decisão na propriedade pecuária. O objetivo deste trabalho foi avaliar as respostas agronômicas e morfofisiológicas de cinco genótipos de Brachiaria spp. e gerar modelos de crescimento baseados em soma térmica e fotoperíodo além de adaptar o modelo CROPGROForragem, visando explorar esses recursos computacionais para estabelecer uma base racional de manejo desses capins. Foram estudados quatro genótipos de Brachiaria brizantha (Hochst. ex A. Rich.) Stapf (Marandu, Xaraés, Arapoty e Capiporã) e uma de B. decumbens Stapf (cv. Basilisk) colhidos a 15 e 7,5 cm durante onze ciclos distribuídos em um ano. As unidades experimentais (parcelas de 9 x 4 m) foram irrigadas e adubadas com o equivalente a 220 kg ha-1 ano-1 de N e K2O. O delineamento experimental foi de blocos completos casualizados em parcelas subdivididas com quatro repetições. Foi calculada a produção total e estacional de forragem. Foram monitoradas uma rebrotação de verão e de inverno, descrevendo-se a massa do resíduo, o fluxo de tecidos, padrão de acúmulo de forragem e as taxas de fotossíntese foliar dos capins, que foram utilizadas para a simulação da taxa de fotossíntese do dossel. Os capins Capiporã e Xaraés foram mais produtivos durante o verão. No inverno não houve diferença na produção dos genótipos. A altura de corte de 7,5 cm proporcionou maior acúmulo de forragem acima do resíduo para todos os genótipos, sendo que nesse regime de corte as plantas apresentaram as maiores taxas de crescimento em nível de perfilho, expressas pela maior taxa de alongamento e aparecimento de folhas. Além disso, houve maior renovação de perfilhos sob essas condições. Apesar dos capins terem produções diferentes, suas taxas de fotossíntese de dossel, foram semelhantes nas duas alturas de corte dentro de cada estação, sugerindo um mecanismo de compensação entre a área foliar e fotossíntese de folhas individuais. Os modelos de acúmulo de forragem baseados em graus dia (GD) e unidades fototérmicas (UF) se ajustaram bem à produção. O uso da UF mostrou-se efetivo na predição de características produtivas dos capins estudados. O resultado da adaptação do modelo CROPGRO-Forragem sugere que essa é uma ferramenta eficiente para integrar aspectos fisiológicos de B. brizantha e pode ser usado para simular o crescimento com boa exatidão.In order to understand and describe forage growth knowledge is needed on their agronomical and physiological aspects as well as their responses to soil and climate. Developing tools that enable the rationalization of these relationships is important both under an academic (e.g., in studies of growth dynamics) and a practical (management and decision making in productions systems) standpoints. The objective of this study was to evaluate the agronomic, morphological and physiological responses of five genotypes of Brachiaria spp. and to develop growth models based on thermal sum (growing degree-days, GDD) and daylength (photothermal unit, PU) as well as to adapt the CROPGRO-Forage model, aiming at evaluating the potential of this resources in forage research and production. Four genotypes of Brachiaria brizantha (Hochst. ex A. Rich.) Stapf (Marandu, Xaraés, Arapoty, and Capiporã) and one of B. decumbens Stapf (Basilisk) were harvested at 15 and 7.5 cm over 11 cycles in one year. The experimental units (9 x 4 m plots) were irrigated and fertilized with the equivalent to 220 kg ha-1 yr-1 of N and K2O. The experimental design was a randomized complete block in split-plot with four replications. Total annual and seasonal forage yields were calculated. One mid-summer and one mid-winter regrowths were monitored weekly to describe stubble mass, tissue flow, forage accumulation rate and the rates of leaf photosynthesis, which were used to simulate canopy photosynthesis. Capiporã and Xaraés were more productive during the summer, but in winter there was no difference among genotypes in yield. Clipping at 7.5 cm yielded more forage for all genotypes and under this management, plants showed the highest growth rates at the tiller level, expressed as higher leaf appearance and elongation rates. There was also higher tiller turnover under these conditions. Despite the differences in forage yield among grasses rates of canopy photosynthesis were similar within the two clipping heights within each season, suggesting the operational of a compensatory mechanism between leaf area and photosynthesis per unit of leaf area. The accumulation models based on GDD and PU showed good fit to observed forage production. The PU model was effective in predicting the productivity of Brachiaria grasses. The adaptation of the CROPGRO-Forage model suggests it is an efficient tool to integrate physiological aspects of B. brizantha and can be used to simulate yield with good accuracy.
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Martín, Matas Guiomar. „Identification of suppressors of the constitutive photomorphogenic phenotype of pifq: from chloroplasts to rhythmic growth“. Doctoral thesis, Universitat de Barcelona, 2015. http://hdl.handle.net/10803/350563.
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Plants germinated in the dark under the soil surface, undergo skotomorphogenic development. Once they emerge from the soil surface perceive light for the first time. Then, seedlings switch from skotomorphogenic to photomorphogenic development, a process that is known as seedling deetiolation. At this moment, seedlings rapidly form the photosynthetic apparatus to start to produce energy and avoid photodamage. In addition, seedlings adapt their morphology to enhance light capture for photosynthesis, inhibiting hypocotyl elongation and stimulating cotyledon separation and expansion. At the molecular level, after first exposure to light, phytochromes photoreceptors interact with a subgroup of transcription factors named PIFs (Phytochrome Interacting Factors). This light-induced interaction between the phytochromes and PIF initiates a cascade of transcriptional changes that allows plants to adjust to the new light environment.
At the beginning of this thesis, the PIF-regulated transcriptional networks that operate during photomorphogenesis had been described. However, the primary target genes that implement downstream cellular functions or amplify the PIF signaling pathway had not yet been identified. Therefore the main goal of this work was to identify the downstream effector genes of the PIF transcription factors to elucidate the PIF-regulated transcriptional network that represses photomorphogenic development. In order to identify new regulators of seedling deetiolation acting downstream of PIF transcription factors, we designed a genetic screen to search for mutants that suppress the constitutive photomorphogenic phenotype of the pifq mutant. Interestingly, our genetic screen has allowed the identification of 23 genes that suppress or enhance the pifq constitutive photomorphogenic phenotype. Among them, we found a big representation of genes codifying for chloroplast proteins, then, we decided to study the interplay of chloroplast biogenesis (a PIF-regulated process) and chloroplast retrograde signaling in the regulation of seedling deetiolation. The work performed allowed us to determine that dysfunctions in chloroplast biogenesis repress photomorphogenesis, through the transcriptional repression of light induced-PIF repressed genes, in order to adjust seedling development to the photosynthetic capacity of the chloroplast. Moreover, we studied the role of CDF5, a transcription factor found in our suppressor screening, and we concluded that the transcriptional regulation of CDF5 by the circadian clock and the PIF transcription factors defines a novel mechanism of regulation of growth under diurnal conditions.Las plantas, durante todo su ciclo vital, adaptan constantemente su desarrollo y crecimiento a los cambios que se producen en el ambiente que las rodea. En periodos de oscuridad, ya sea cuando las plantas germinan bajo la superficie de la tierra o cuando crecen en condiciones diurnas (noche y día), se acumulan en el núcleo los factores de transcripción llamados Phytochrome Interacting Factors (PIFs). Estos factores de transcripción regulan la expresión de miles de genes con el fin de inducir crecimiento y reprimir el desarrollo fotomorfogénico. Tras la germinación en oscuridad, una vez las plantas alcanzan la superficie y se exponen a la luz, los fitocromos, fotoreceptores que perciben la presencia de luz roja y roja lejana, se activan y se traslocan al núcleo, donde inducen la degradación de los factores de transcripción PIFs. La degradación de estas proteínas produce amplios cambios en el transcriptoma de las plantas induciendo el desarrollo fotomorfogénico. Al inicio de la tesis, las redes transcripcionales reguladas por los PIFs habían sido descritas, sin embargo, los genes que inician la cascada transcripcional que implementan las funciones celulares que permiten a las plantas desarrollarse fotomorfogénicamente eran desconocidos. Nuestro principal objetivo ha sido determinar estos genes, para ello realizamos un cribado de supresores del fenotipo fotomorfogénico constitutivo de plantas mutantes de genes PIF. Los resultados del cribado nos han permitido identificar nuevos reguladores del desarrollo fotomorfogénico que actúan como intermediarios de la cascada de señalización iniciada por los factores de transcripción PIF. Estos resultados permiten ampliar el conocimiento de la función transcripcional de los PIFs, mostrando ejemplos específicos de genes tanto inducidos como reprimidos. Además, nuestro trabajo ha permitido determinar que la biogénesis del cloroplasto es un proceso necesario para que las plantas se desarrollen fotomorfogénicamente.
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Williamson, Michelle Leigh. „Differential responses of tillers to floral induction in perennial ryegrass (Lolium perenne L.) : implications for perenniality : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Plant Biology at Massey University, Palmerston North, New Zealand“. Massey University, 2008. http://hdl.handle.net/10179/842.
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Development of sustainable practices is an important goal in agriculture. One possibility involves the development of perennial cereal crops, but the mechanisms of perenniality first need to be understood. While in annual cereals flowering structures die following seed production, in perennial grasses, perenniality is achieved by maintaining at least one shoot in a vegetative state. There are two views on perennating tiller origin in perennial grasses: some authors suggest that all over-wintering tillers flower in spring and summer, leaving spring-initiated tillers to perennate, while others indicate that spring-initiated tillers are too immature to survive summer conditions, thereby implying that flowering must be prevented in some over-wintering tillers. An understanding of perenniality will therefore require an understanding of flowering control in these species. Temperate perennial grasses have dual induction requirements for flowering, where plants become competent to perceive inductive signals following vernalisation, and flowering is initiated by inductive photoperiods. Two hypotheses were formulated to test these models. The ‘environmental control hypothesis’ stated that all adequately vernalised perennial ryegrass tillers would flower on sufficient exposure to inductive photoperiods. Alternatively, the ‘spatial control hypothesis’ stated that in addition to the environmental mechanisms, a spatial control mechanism acts to regulate flowering. Two experiments were conducted to test these hypotheses. Perennial ryegrass and Italian (annual) ryegrass were induced to flower and differences between the annual and perennial habits at flowering time were observed. However neither hypothesis was proven. In the second experiment, flowering was studied in detail in individual tillers of perennial ryegrass. The eldest tiller flowered in all flowering plants. The second eldest tiller did not flower in 72% of plants with more than one reproductive tiller, while the third eldest tiller flowered in 94% of these plants. These data favour the spatial control hypothesis which suggests that a spatial regulatory mechanism might act to repress flowering in some competent perennial ryegrass tillers. These results were supported by studies of meristem morphology and by a preliminary gene expression study. Maintenance of older, established tillers in a vegetative state might allow the perennial plant a greater chance of survival during summer.
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Berghauer, Erika Mary. „Photoperiodic response of commercial calibrachoa cultivars“. [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0007015.
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Carvalho, Rogério Falleiros. „Uso de mutantes fotomorfogenéticos no estudo da competência para regeneração in vitro em micro-tomateiro (Lycopersicon esculentum CV Micro-Tom“. Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-19022004-103113/.
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Paralelamente ao modelo Arabidopsis thaliana, o tomateiro (Lycopersicon esculentum) tem sido crescentemente utilizados em abordagens genéticas de questões fisiológicas. Uma das principais vantagens de Arabidopsis como planta de laboratório tem sido seu pequeno porte e ciclo de vida curto. Contudo, a cultivar Micro-Tom (MT) de tomateiro possui tamanho muito reduzido (8 cm) e pode produzir até 5 gerações por ano. Mutantes fotomorfogenéticos em tomateiro deficientes na síntese do cromóforo do fitocromo (au), mutantes deficientes na síntese das apoproteínas PHYA e PHYB1 (fri e tri, respectivamente) e mutantes superexpressando o fitocromo (hp, atv e Ip) constituem-se em um modelo para estudos da fotomorfogênese. No que se refere à capacidade de regeneração in vitro como uma resposta fotomorfogenética, poucos trabalhos têm sido realizados. O presente trabalho teve como objetivo transferir as mutações au, fri, tri, hp, Ip e atv, bem como o locus de regeneração (Rg1) da cultivar MsK, para a cultivar Micro-Tom. As linhagens obtidas foram utilizadas para verificar o efeito da fotomorfogênese na competência para regeneração in vitro. Para tanto, foram realizados tratamentos com luz branca, vermelho (V) e vermelho-extremo (VE) em explantes radiculares, caulinares e foliares do genótipo micro-MsK em meio MS mais 5mM de BAP e tratamentos com luz branca em explantes radiculares, caulinares e foliares de micro-mutantes fotomorfogenéticos também em meio MS mais 5mM de BAP. Para todos os tratamentos utilizou-se a cultivar MT como controle. Sob V, as raízes de micro-MsK apresentaram-se diferenciadas, enquanto sob VE não ocorreu diferenciação. O maior número de gemas formadas tanto para caule quanto para folhas de micro-MsK ocorreu sob V, enquanto sob VE foi observado um decréscimo na formação de gemas. A partir destes resultados sugere-se que a forma ativa do fitocromo, induzida pelo V, interage com o Rg1 na aquisição de competência para regeneração. Nos tratamentos com luz branca, raízes de micro-MsK e de mutantes micro-hp, micro-atv e micro-Ip apresentaram-se diferenciadas, enquanto não houve diferenciação para o mutante micro-au ou para o controle MT. O número de gemas formadas alcançou maiores valores para folhas de micro-hp e micro-Ip e a para caules de micro-atv. Apenas um número muito reduzido de gemas foi formado a partir de folhas de micro-au. Com base na alta competência para regeneração de micro-MsK e de mutantes que superexpressam o fitocromo, sugere-se que o fitocromo promove, em uma via de sinalização, a indução de fatores de regeneração (Rg1). Alternativamente, o locus Rg1 poderia promover a alta capacidade regenerativa tornando os explantes mais competentes ao efeito da superexpressão do fitocromo, o qual poderia induzir outros fatores de regeneração.Parallel to Arabidopsis thaliana model, the tomato (Lycopersicon esculentum) has been increasingly used as a genetic approach to address physiological questions. One of the main advantages of Arabidopsis as a laboratory plant has been its small size and short life cycle. However, the tomato cultivar Micro-Tom (MT) possesses reduced size (8 cm) and can produce up to 5 generations per year. Tomato photomorphogenic mutants deficient for the synthesis of phytochrome chromophore (au) or the apoprotein PHYA and PHYB1 (fri and tri, respectively), as well as mutants superexpressing phytochrome (hp, atv and Ip) consist on a model to study photomorphogenesis. Concerning the in vitro regeneration capacity as a photomorphogenic response, fewer works have been carried through. The current work aimed at transfering the mutations au, fri, tri, hp, Ip and atv, as well as the regeneration locus (Rg1) of cv MsK to the cv Micro-Tom (MT). The genotypes obtained were used to verify the effect of photomorphogenesis on the competence for in vitro regeneration. Root, stem and leaf explants from MT and Micro-MsK were incubated in MS plus 5mM BAP under white, red (R) and far-red (FR) light. Root, stem and leaf explants from MT and photomorphogenic micro-mutants were incubated in MS plus 5mM BAP under white light. Under R, roots of micro-MsK were presented differentiation, while under FR the differentiation did not occur. Under R, stem explants from micro-MsK formed more shoots than did leaf explants, while under FR was observed a decrease in shoot formation for all types of explants. These results suggest that the active form of phytochrome, induced by R, interacts with the Rg1 in the acquisition of competence for regeneration. In the treatments with white light, roots of micro-MsK and of mutants micro-hp, micro-atv and micro-Ip presented differentiation, while no differentiation was observed for the mutant micron-au or control MT. The number of shoots formed reached the highest values for leaf explants of micro-hp and micro-Ip and for stem explants of micron -atv. Only a low number of shoots was formed from micro-au leaf explants. On the basis of the high competence for regeneration of micro-MsK and mutants that super express phytochrome, it is suggested that the phytochrome promotes, in a signaling pathway, the induction of regeneration factors ( Rg1 ). Alternatively, the Rg1 locus may turn the explant most competent to respond to phytochrome, which could induces others regeneration factors.
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Clokie, Benjamin Gregory James. „Impact of lighting conditions on the developmental physiology of Atlantic salmon (Salmo salar)“. Thesis, University of Stirling, 2017. http://hdl.handle.net/1893/27240.
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The Atlantic salmon (Salmo salar) lifecycle is punctuated by distinct ontogenic stages which are routinely manipulated commercially by photoperiod regimes to enable year-round production. As such, light plays a critical role throughout the production cycle, however, it remains poorly characterised and light spectrum and intensity have not been defined optimally yet. This thesis was therefore set out to test the effects of narrow bandwidth light (Blue-λ(max) 444 nm, Green-λ(max) 523 nm, Red-λ(max) 632 nm and White) and intensity in freshwater (FW). Fry-parr development, out-of-season smoltification and ocular and vertebral health were examined as was the long-term effects of FW light regimes on seawater (SW) growth and muscle structure. In addition, the impact of photoperiod regimes on out-of-season smolts following transfer to SW was investigated.
Major findings from the trials conducted show that light spectrum and intensity influence parr development with lower intensities performing better than higher intensities. Both the initiation and duration of smoltification was impacted by spectrum. Importantly, this doctoral work showed that daily changes in light intensity, from low during the scotophase to high during the photophase applied for the duration of a standard out-of-season smoltification regime was capable of providing a sufficient cue for the induction of smoltification. Historic FW light exposure impacted SW performance and post-transfer SW photoperiod had significant impact upon growth and maturation development. Results based on changes to the gonadosomatic index provide important guidance for suitable post-transfer photoperiods for smolt transferred to SW around the winter solstice. Importantly, from the parameters tested, exposure to different spectrum or light intensities did not adversely affect vertebral or ocular health.
This thesis did not only focus on the physiological effects of light but also aimed to characterise better the pathways involved in light perception and integration. To do so, the neural response to both broad spectrum white light, darkness and Red and Blue light was investigated through deep brain insitu-hybridisation and high throughput sequencing (NGS) of the pituitary gland. Results showed substantial spectral and light/dark changes in the both the deep brain and pituitary transcriptome. Overall, this research provides both scientifically interesting and commercially relevant guidance for the optimisation of lighting systems for use in captive salmon aquaculture. Major findings from the trials conducted show that light spectrum and intensity influence parr development with lower intensities performing better than higher intensities. Both the initiation and duration of smoltification was impacted by spectrum. Importantly, this doctoral work showed that daily changes in light intensity, from low during the scotophase to high during the photophase applied for the duration of a standard out-of-season smoltification regime was capable of providing a sufficient cue for the induction of smoltification. Historic FW light exposure impacted SW performance and post-transfer SW photoperiod had significant impact upon growth and maturation development. Results based on changes to the gonadosomatic index provide important guidance for suitable post-transfer photoperiods for smolt transferred to SW around the winter solstice. Importantly, from the parameters tested, exposure to different spectrum or light intensities did not adversely affect vertebral or ocular health. This thesis did not only focus on the physiological effects of light but also aimed to characterise better the pathways involved in light perception and integration. To do so, the neural response to both broad spectrum white light, darkness and Red and Blue light was investigated through deep brain insitu-hybridisation and high throughput sequencing (NGS) of the pituitary gland. Results showed substantial spectral and light/ dark changes in the both the deep brain and pituitary transcriptome. Overall, this research provides both scientifically interesting and commercially relevant guidance for the optimisation of lighting systems for use in captive salmon aquaculture.