Zeitschriftenartikel zum Thema „Persicaria perfoliata“

Abstract The introduced invasive vine mile-a-minute weed Persicaria perfoliata (formerly Polygonum perfoliatum) is continuing to spread throughout the Mid-Atlantic states and beyond, from its initial site of introduction near York, Pennsylvania. This weed frequently interferes with forest regeneration and until recently had few natural enemies attacking it in its introduced range. In 2004, a permit for field release of a host-specific Asian weevil Rhinoncomimus latipes was obtained, and the weevil has since been released in five states. The weevil has established well and is beginning to impact growth and development of the weed.

Seven species, viz., Calamagrostis stoliczkai Hook. f., Juncus bracteatus Buchen., Matthiola flavida Boiss., Parrya nudicaulis (L.) Regel, Persicaria perfoliata (L.) H. Gross, Ptilotrichum canescens (DC.) C. Meyer and Stellaria tibetica Kurz have been recorded from Pin Valley National Park, Lahaul-Spiti as new additions to the flora of Himachal Pradesh.

AbstractMile-a-minute weed or devil's tearthumb (Polygonum perfoliatum, syn. =Persicaria perfoliata) is an invasive annual vine in the Mid-Atlantic and Northeastern United States that reproduces solely through seeds. Our study aimed to identify how mile-a-minute seed viability is affected by time of year and the maturity of the fruit surrounding the achene. Full-sized immature (green) and mature (blue) fruits were collected from five field sites every 2 wk over a 3 mo period, and seed viability was assessed using a triphenyl tetrazolium chloride (TZ) assay. At the onset of seed production in mid-August, 35% of seeds from immature fruits were viable. This percentage increased steadily, peaking at 84% in late September before declining at some sites around the time of the first frost. In contrast nearly all seeds with mature fruits (96%) were viable at all collection dates. Thus land managers who apply physical or chemical control methods for mile-a-minute weed should do so before the onset of any seed production and not simply before fruit maturation. If it is necessary to apply control methods after fruit set, it should be done as early in the season as possible.

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

Mile-a-minute (Persicaria perfoliata (L.) H. Gross; family: Polygonaceae) is an exotic annual barbed vine that has invaded the northeastern USA and Oregon (2). In July of 2010, in a search for potential biological control pathogens (3), diseased P. perfoliata plants were found along the Firtina River near Ardesen, Turkey. Symptoms were irregular dark necrotic lesions along leaf margins and smaller irregular reddish lesions on the lamellae of leaves. Symptomatic leaves were sent to the quarantine facility of FDWSRU, USDA, ARS in Ft. Detrick, MD, for pathogen isolation and testing. Symptomatic leaves were excised, surface disinfested in 0.615% NaOCl, and then incubated for 2 to 3 days in sterile moist chambers at 20 to 25°C. Numerous waxy sub-epidermal acervuli with 84-μm-long (mean) black setae were observed in all of the lesions after 2 to 3 days of incubation. Conidiophores within acervuli were simple, short, and erect. Conidia were one-celled, hyaline, guttulate, subcylindrical, straight, 12.3 to 18.9 × 3.0 to 4.6 μm (mean 14.3 × 3.7 μm). Pure cultures were obtained by transferring conidia onto 20% V-8 juice agar. Appressoria, formed 24 h after placing conidia on dialysis membrane over V-8 juice agar, were smooth, clavate, aseptate, regular in outline, and 6.4 to 10.0 × 5.1 to 7.2 μm (mean 7.5 × 6.6 μm). These characters conformed to the description of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. (1). A voucher specimen was deposited in the U.S. National Fungus Collections (BPI 882461). Nucleotide sequences for the internal transcribed spacers (ITS 1 and 2), directly sequenced from ITS 1 and ITS 4 standard primers (4), were deposited in GenBank (JN887693). A comparison of these sequences with ITS 1 and 2 sequences of the C. gloeosporioides epitype IMI 356878 (GenBank EU 371022) (1) using BLAST found 479 of 482 identities with no gaps. Conidia from 14-day-old cultures, in an aqueous suspension of 1.0 × 106 conidia ml–1, were spray-inoculated onto healthy stems and leaves of twenty 30-day-old P. perfoliata plants. Another 10 plants were not inoculated. All plants were placed in a dew chamber at 25°C for 16 h with no lighting. They were then placed in a 20 to 25°C greenhouse with a 14-h photoperiod. Light was generated using 400W sodium vapor lights. Lesions developed on leaves and stems of all inoculated plants after 7 days, and symptoms were the same as observed in the field. Each plant was rated weekly for disease severity on a 0 to 10 rating scale where 0 = no disease symptoms and 10 = 100% symptomatic tissue. After 28 days, the average disease rating of inoculated plants was 3.95 ± 0.94. No disease developed on noninoculated plants. C. gloeosporioides was reisolated from all inoculated plants. Host range tests will determine the potential of this isolate as a biological control agent for P. perfoliata. To our knowledge, this is the first report of anthracnose caused by C. gloeosporioides on P. perfoliata. References: (1) P. F. Cannon et al. Mycotaxon 104:189, 2008. (2) J. T. Kartesz and C. A. Meacham. Synthesis of the North American Flora, Version 1.0., North Carolina Botanical Garden, Chapel Hill, N.C. 1999. (3) D. L. Price et al. Environ. Entomol. 32:229, 2003. (4) T. J. White et al. PCR Protocols: A Guide to Methods and Applications. Academic Press, Inc., San Diego, CA, 1990.

This study analyzed the flora, life form, and vegetation of the Nakdong River wetland. Vegetation analysis was performed on 37 plots using the phytosociological method of the Zürich-Montpellier School. PCA analysis was conducted by using the vegetation data (ground cover of class; 1~9) of 37 plots surveyed by phytosociological method. PCA (Principal Component Analysis) was used to statistically analyze the objectivity of the community classification and the character species. The traditional classification and mathematical statistic methods were used. A total of 82 taxa belonging to 28 families, 65 genera, 72 species, 2 subspecies, and 8 varieties were present in the vegetation of the survey area. The life form was analyzed to be the Th-R5-D4-e type. The communities were classified into seven communities: Miscanthus sacchariflorus community, Phragmites communis community, Phragmites communis–Carex dispalata community, Ulmus parvifolia community, Zizania latifolia community, Setaria viridis community, and Salix koriyanagi–Salix chaenomeloides community. As a result of PCA analysis, it was classified into seven communities. Seven communities were analyzed, where the most dominant species (M. sacchariflorus, P. communis, C. dispalata, U. parvifolia, Z. latifolia, S. viridis, S. koriyanagi, S. chaenomeloides) of each community were examined as character species. Another species is analyzed as Salix koreensis. Of the sixteen M. sacchariflorus communities, Pterygopleurum neurophyllum was present in six plots (A-2 group) but not in ten plots (A-1 group). These two groups showed differences in coverage and the number of occurring species. As for the relative net contribution degree (r-NCD) in the A-2 group, most species showed low r-NCD except for M. sacchariflorus, which showed an r-NCD of 100. The r-NCDs in the A-1 group were as follows: Miscanthus sacchariflorus (100), P. neurophyllum (21.74), and Persicaria perfoliata (10.14). Therefore, P. neurophyllum is difficult to grow in the A-1 group. As a result, it is thought that the high density of M. sacchariflorus affects the growth and distribution of P. neurophyllum. In order to expand and maintain P. neurophyllum, the habitat environment needs to be altered by adjusting the density of M. sacchariflorus.

The chemical composition of hydrophytes is affected by many environmental and physiological factors. The content of certain nutrients and micro components in the tissues of four hydrophytes, Persicaria senegalensis L., Ceratophyllum demersum L., Potamogeton crispus L. and P. perfoliatus L., was investigated over four seasons. These hydrophytes were collected from different sites in the Nile River in Aswan subjected to domestic-like, industrial and agricultural pollutions and non-polluted conditions. The plant tissues were digested and nitrate (NO3-), phosphate (PO43-), sulfate (SO42-), calcium (Ca2+), magnesium (Mg2+), chloride (Cl-), fluoride (F-) and bromide (Br-) were measured using atomic absorption spectroscopy. The distribution of the different hydrophytes was related to different levels or types of pollution. Persicaria senegalensis was the most tolerant of the pollution conditions that appeared in all sites during all seasons. Both pollution and seasonal variability affected the concentration of different nutrients in the tissues of the studied hydrophytes. In general, the concentration of all nutrients increased in the polluted conditions. The concentrations of NO3- and PO43- were low comparing to other nutrients in both water samples and plant tissues. The highest concentrations of SO42- were detected in summer for all studied hydrophytes. The highest concentrations of Ca2+ and Mg2+ in P. senegalensis were measured in autumn and spring, respectively. The seasonal maxima of the concentrations of Ca2+ and Mg2+ of C. demersum, P. crispus and P. perfolitas were detected in winter, spring and autumn, respectively. In conclusion, Persicaria senegalensis was proven to have the widest range of tolerance of water quality, suggesting it could be a more reliable bio-indicator than the other three hydrophytes.

Fatty acids, as part of cell membranes, determine their fluidity and dielectric constant. In addition, they play an important role in human and animal nutrition. This work examines the composition and fatty acid content of dried higher aquatic plants from Lake Kotokel (Republic of Buryatia, Russia) and their potential as a raw material for the production of feed and biologically active food supplements. A modified Bligh-Dyer method was used to extract lipid fraction. Fatty acids in the form of their methyl esters were analysed by gas chromatography–mass spectrometry. Palmitic C16:0 acid was the main saturated fatty acid found in Myriophyllum sibiricum, Elodea canadensis, Persicaria amphibia and Potamogeton perfoliatus in Lake Kotokel. The main unsaturated acid found in plants, regardless of their type, was the α-linolenic C18:3-ω3 acid. In addition, a relatively high content of stearic C18:0, myristic C14:0 and pentadecanoic C15:0 acids was noted. Branched acids (i-C14:0, i-C15:0, i-C16:0, i-C17:0, i-C20:0, a/i-C15:0, a/i-C17:0 and 10Me-C16:0) and odd carbon numbered acids (C13:0, C15:0, C17:0, C19:0, C21:0 and C23:0) were identified. An analysis of the authors’ and literature data revealed that the studied species of higher aquatic plants in reservoirs in the Asian part of Russia are characterised by a high content of α-linolenic C18:3-ω3 (up to 56%) and a low content of oleic C18:1-ω9 (up to 1.81%) acids. The content of α-linolenic C18:3-ω3 acid in plants from reservoirs in the European part of Russia was lower (up to 42%), while that of oleic acid C18:1-ω9 was higher (up to 14%).

The Bolshoy Irgiz River is 375 km long and has a catchment area of 24 thousand km, including the Orenburg Region and the Samara Region. The flora contains 64 species of plants: 14 belong to the class of monocotyledons (Liliopsida) and 48 belong to the class of dicotyledons (Magnoliopsida), the most numerous families by the number of species are Lamiaceae (5), Cyperaceae (5), Potamogetonaceae (5), Polygonaceae (4), Poaceae (4). The ecological spectrum of the flora is represented by hydrophytes (20 species), helophytes (8), hygrohelophytes (5), hygrophytes (19) and mesophytes (12). These are mainly broad-area species of the Eurasian (23), Holarctic (23) and pluriregional (11) ranges. There are only 7 species of plants with narrow range borders. In economic terms the flora consists of 28 species of medicinal plants, as well as forage (23), tanning (15), food (14), dye (13), honey (12), ornamental (11) and poisonous (8) plants. Vegetation is represented by 17 formations and is represented by the coastal-aquatic vegetation type and two subtypes: coastal and aquatic vegetation. The first subtype contains formations: Phragmiteta australis, Typheta angustifolitae, Scirpeta lacustris, Sagittarieta sagittifoliae, Persicarieta amphibii, Alismateta plantago-aquaticae. The second subtype contains: Nymphaeeta candidae, Nuphareta luteae, Potameta lucentis, Potameta perfoliati, Potameta crispus, Lemneta minori, Salvinieta natantis, Hydrocharieta morsus-ranae, Spirodeleta polyrhizi, Lemneta trisulcae, Ceratophylleta demersi. Coastal plant communities are characterized by a 23-tier structure, a high degree of projective coverage, and a relatively high abundance of species (from 15 to 26). Water phytocoenoses are poor in species composition, have a spotty character of overgrowth and 12 tiered structure. The Irgiz Floodplain nature monument requires compliance with the stipulated protection measures in order to preserve the habitat of rare plant populations Nuhar luteum L., Nymphaea candida J. et C. Presl., Salvinia natans L. (All.), Cicuta virosa L., Najas major All. Descriptions of phytocenoses were carried out according to the generally accepted method of geobotanical research in the summer of 2018, while tiering, abundance, projective cover and vitality were noted as well as all species included in plant communities were registered.

Aquatic and semi-aquatic communities of Koryak national district are so far studied very poorly. Only B. Tikhomirov (1935) had cited few data on the aquatic vegetation of Penzhina River basin. This paper presents an analysis of 68 relevés (obtained in 2011–2016) of aquatic, littoral and shore vegetation in the surroundings of Talovskoye lake, the largest lake of the Parapolskiy dol area (Penzhinskiy district, Kamchatka Territory). The plant community classification was elaborated using the Russian school dominant-determinant approach. The vegetation of water area and lake shores is referred to Potamogetonetion, Limoselletion, Phragmitetion vegetation types; that of flood-plain terraces to Festucetion pratensis and Salicetion. 23 formations and 30 associations (including 2 new ones) were characterized. The vegetation cover of Talovskoye lake and its surroundings is low in species number and community diversity. Oligotrophic communities, widely spread around Northern Eurasia, predominate. Oligotrophic species are abundant in the aquatic communities while oligomesotrophic and mesotro­phic ones prevailed in oxbows and flood-plain lakes. The high abundance of Arctophila fulva, Batrachium trichophyllum, Callitriche palustris, Caltha palustris subsp. sibirica, C. natans, Deschampsia komarovii, Eleocharis acicularis, Glyceria lithuanica, Hippuris vulgaris, Limosella aquatica, Myriophyllum verticillatum, Persicaria lapathifolia, Potamogeton gramineus, Ranunculus gmelinii, R. reptans, Sparganium angustifolium, S. emersum, S. hyperboreum, S. natans, Subularia aquatica is common in seasonally drained sites of the lake bottom. The shores of small-size lakes (250–300 m in diameter) are usually low and swampy. Hydrophyte vegetation is represented by pondweed (Potamogeton gramineus, P. perfoliatus) and bur-reed communities (Sparganium emersum, S. angustifolium, S. hyperboreum). The onshore helophyte communities dominated by Carex rhynchophysa, C. rostrata, Comarum palustre, Eriophorum polystachyon, Hippuris vulgaris, and Menyanthes trifoliata form narrow strips. Underdeveloped aquatic and semi-aquatic littoral vegetation is character for medium-size lakes (300–700 м in diameter). The helophyte border (up to 3 m wide) is well developed along the whole lake shore line. Sometimes the swampy shores and quagmires with the predominance of Menyanthes trifoliata are met; occasionally the stretches of boggy shores with the predominance of cotton grass (Eriophorum)–Sphagnum-rich communities occur. The lakes of medium-size are filled by well-developed aquatic and littoral ve­getation. About 30 % of shoreline is occupied by wide (10 m wide and more) helophyte stripe formed by the thickets of Equisetum fluviatile, Arctophila fulva, Cicuta virosa, Comarum palustre, Carex rhynchophysa, C. rostrata. The hydrophyte communities­ are made by pondweeds (Potamogeton gramineus, P. perfoliatus), Myriophyllum verticillatum, sometimes by Utricularia intermedia, U. macrorhiza that formed thickets in the water profile. On the lake shores, lowland grass meadows with the predominance of Arctophila fulva, Glyceria lithuanica, Deschampsia komarovii occurred as well as shrubby willow thickets (Salix pulchra). Grass meadows (Calamagrostis purpurea subsp. langsdorffii) and large-sedge communities are common on the lake shores. The large-size lakes (more than 700 m in diameter) have the vast areas of shallow water. These are the ­areas of littoral helophyte communities. Monodominant thickets of Arctophila fulva and Equisetum fluviatile occupy the shallow waters rich by organic matter. On seasonally drained clay and sandy loam bottoms, Senecio palustris predominates. Along the shores wide strips of sedge hummocks (Carex appendiculata) stretched alternating with the patches of swampy sedge communities (C. cryptocarpa, C. rostrata) with admixture of Cicuta virosa. Further from the lake shores, the flooded grass meadows and shrub willow thickets are common. The long-drained lake basins are occupied by Arctophila fulva, sedge communities and hygrophilous herbs. Floodplain grass-rich meadows (Calamagrostis purpurea subsp. langsdorffii, Glyceria lithuanica, Deschampsia komarovii) and hummocky sedge communities (Carex appendiculata) covered the low shores of Talovskoye lake. The vast shallow water zone adjoined to the shores is occupied by the monodominant helophytic communities (Arctophila fulva, Caltha natans, Hippuris vulgaris, Equisetum fluviatile) and hydrophytic communities dominated by Sparganium emersum, S. angustifolium, S. hyperboreum, S. natans, Potamogeton gramineus, P. perfoliatus, Myriophyllum verticillatum and others. The Lake is surrounded by shrubby willow thickets (Salix pulchra) with the grass layer formed by Calamagrostis purpurea subsp. langsdorffii, Rubus arcticus, Carex cryptocarpa. The survey of the vegetation units of Talovskoye lake and its surroundings had revealed 4 vegetation types, 23 formations and 30 associations, according to dominant-determinant approach. There are communities of 19 formations and 21 associations on the waters of the Lake and on its drained bottom; of 2 formations and 5 associations in the floodplain ponds and oxbows; of 2 formations and 4 associations on the low lakeside terraces. Hygromesophyte grass meadows (Calamagrostis purpurea subsp. langsdorffii) and hygrophyte sedge and grass stands (Glyceria lithuanica, Carex appendiculata) formed the plant cover of low terraces. There are also communities of willow shrubs (Salix pulchra) which are not discussed in the present paper. The main characteristic features of the syntaxonomic composition of the aquatic, littoral and shore vegetation of Talovskoye lake are the absence of numerous syntaxa that are common for the Kamchatka Peninsula: nymphaea (Nupharetosa) and free-floating aquatic vegetation (Lemnetosa minoris); communities of underwater rooting spore-bearing plants (Isoёtоsa). The low community diversity of submerged rooted plants (Potamogetonetosum) is noticed. So, in Talovskoye lake several pondweed formations (Potamogetoneta pectinati, P. pusilli, P. praelongi, P. friesii) widely spread in Eurasia and Kamchatka Peninsula, were not found. Two new syntaxa — Deschampsietum komarovii ranunculosum gmelinii and Glycerietum lithuanicae deschampsiosum komarovii — are suggested for the damp meadows with the predominance of Deschampsia komarovii and Glyceria lithuanica. The communities formed by D. komarovii are endemic for the north of the Far East. The lack of the published relevѐs of these communities confirmed their poor knowledge. Glyceria lithuanica has Eurasiatic areal but it is rarely recorded as edificator. In some regions of the European Russia, this species, considered as the very rare, is included into regional Red Data Books (Skvortsov et al., 2000). The main part of formations and associations found for Talovskoye lake and its surroundings had large distribution areas due to the wide geographical distribution of the dominants and the similarity of the ecological conditions of aquatic and littoral habitats of different regions of Northern Eurasia. The formation Deschampsieta komarovii is considered to be endemic for the North of the Far East. Unusually dry season of 2016 allowed describing the communities of herbaceous annuals (ephemeras). They were assigned to the special vegetation type Limoselletion. A new class of formations Callitrichetosa palustris, united the aquatic communities with the predominance of rooting perennial amphibian plants adapted to periodic drying, is distinguished. Together with the communities of annual hygrophytes, it corresponds to the alliance Eleocharition acicularis Pietsch ex Dierßen 1975 (Dierßen, 1996; Šumberová, 2011; Šumberová et al., 2011a, b). The resemblance of a large part of syntaxa of aquatic and semi-aquatic communities in two classifications may be explained by their mono- or oligodominance and low species diversity.

Polygonaceae is a large family of medicinal herbs that includes many species used as traditional Chinese medicine, such as Persicaria perfoliate. Here, we sequenced the complete cp genome of P. perfoliata using Illumina sequencing technology with the purpose to provide a method to facilitate accurate identification. After being annotated, the cp genome of P. perfoliata was compared with Fagopyrum tataricum, Persicaria chinensis, Fagopyrum dibotrys and Fallopia multiflora. The complete cp genome of P. perfoliata is 160,730 bp in length, containing a small single copy (SSC) region of 12,927 bp, a large single copy (LSC) region of 85,433 bp and a pair of inverted repeats (IR) regions of 62,370 bp. A total of 131 genes were annotated, including eight rRNA genes, 34 tRNA genes and 84 protein-coding genes. Forty-two simple sequence repeats and fifty-five repeat sequences were identified. Mutational hot spots analyses indicated that five genes (matK, ndhF, ccsA, cemA, rpl20) could be selected as candidates for molecular markers. Moreover, phylogenetic analysis showed that all the Polygonaceae species formed a monophyletic clade, and P. perfoliata showed the closest relationship with P. chinense. The study provides valuable molecular information to accurately identify P. perfoliata and assist in its development and application.

This datasheet on Persicaria perfoliata covers Identity, Overview, Distribution, Dispersal, Hosts/Species Affected, Diagnosis, Biology & Ecology, Environmental Requirements, Natural Enemies, Impacts, Uses, Prevention/Control, Further Information.

Abstract Rhinoncomimus latipes Korotyaev is a specialist biocontrol agent of mile-a-minute weed, Persicaria perfoliata (L.) H. Gross (Caryophyllales: Polygonaceae). Currently, R. latipes is released by hand where the presence of the weed is readily detected. However, the hand-release method is not applicable to weed patches spread in hard-to-access areas. This study was conducted to develop a spatially targeted biocontrol strategy by using an unmanned aircraft system (UAS, a.k.a. drone) for the detection of P. perfoliata and aerial release of R. latipes. A ground survey was performed to locate P. perfoliata patches and then a rotary-wing UAS was flown at 15 different altitudes to determine the detectability of P. perfoliata patches. We developed an insect-release system including a pod that housed R. latipes for aerial release. The pod was 3D printed with biodegradable polyvinyl alcohol (PVA), and field tests were conducted to determine the ability of R. latipes to escape the pod and assess their post-release mortality and feeding ability. The results of this study showed that P. perfoliata patches were readily detectable on the aerial images taken at ≤15 m above the ground. More than 98% of R. latipes (n = 118) successfully escaped from the pod within 24 h after aerial deployment. There were no significant (P > 0.05) effects of PVA exposure on the mortality and feeding ability of R. latipes. These results indicate that aerial detection of P. perfoliata and deployment of R. latipes for spatially targeted biological control in hard-to-access areas can be accomplished using a rotary-wing UAS.

In august 2018, fauna of aquatic macroinvertabrates from Persicaria amphibia (L.) Delarbre, Butomus umbellatus L. and Potamogeton perfoliatus L. was studied in Kubenskoe Lake as well as in Vozhe Lake and Mologa River in order to compare the phytophilic and bottom fauna of heterogeneous water bodies. Macrophyte-associated invertebrates and samples of zoobenthos in thickens (in total 37 samples were analyzed) were collected. 68 species of aquatic invertebrates were recorded, including 49 species from Kubenskoe Lake, 41 from Mologa River, and 31 from Vozhe Lake. In the thickets of three macrophyte species, less than a third of all macroinvertebrate richness from the investigated water bodies is recorded. The most abundant species were Endochironomus albipennis Meig., Glyptotendipes gripekoveni Kief., Cricotopus gr. sylvestris. By using cluster analysis, the fauna of all biotopes was divided into macrophyte-associated and bottom-associated. The abundance and biomass of invertebrates in zoophytos in most cases is 3–15 times higher than in zoobenthos. In lakes, species diversity in zoophytos is 1.5–5 times lower than in soil. In the Mologa River the species diversity of zoophytos, on the contrary, is slightly higher. In lakes in the trophic structure, filter-collectors predominate. In the Vozhe Lake a high proportion of predators in the biomass is also recorded. In the Mologa River the trophic structure is more various: along with the collector-filterers, scrapers, shredders and predators are represented. In comparison with research of the 1970s, community structure of macrophyte-associated invertebrates in Vozhe Lake has not practically changed. The dominance of E. albipennis was detected in the Kubenskoe Lake and that was not previously indicated for this reservoir. The species composition and abundance of aquatic macroinvertebrates is determined by the type of substrate (soil or plant), while the structure of communities depends primarily on a type of reservoir, and not on a type of thicket.