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Zeitschriftenartikel zum Thema "Periodontal ligament Blood-vessels"

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Brahmanta, Arya, Sutjipto Sutjipto und Ida Bagus Narmada. „Histological changes during orthodontic tooth movement due to hyperbaric oxygen therapy“. Dental Journal (Majalah Kedokteran Gigi) 49, Nr. 2 (14.02.2017): 63. http://dx.doi.org/10.20473/j.djmkg.v49.i2.p63-66.

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Background: Mechanical force of orthodontics causes changes in periodontal ligament vascularization and blood flow, resulting in biochemical and cellular changes as well as changes in the contour of the alveolar bone and in the thickness of the periodontal ligaments. Hyperbaric oxygen (HBO) therapy is one of many solutions stimulating the growth of new blood vessels and increasing tissue oxygenation. Thus, HBO plays a role in recovery of periodontal ligament and osteoblasts. Purpose: This study aimed to determine the effects of HBO therapy for seven days on periodontal ligament size and osteoblast number in the tension site during bone remodeling in tooth movement. Method: The study was true experimental laboratories with completely randomized control group post test only design. Twenty-four males guinea pigs were randomly divided into three groups. K0 was the control group without any treatment, K1 was the group given a mechanical orthodontic pressure, and K2 was the group treated with the addition of hyperbaric oxygen therapy. The maxillary incisors were moved distally by elastic separator. After HBO therapy on day 7, all of the groups were sacrificed, and then periodontal ligament size and osteoblast number were analyzed by one-way Anova and LSD statistical tests. Result: The results showed significant differences in the size of the periodontal ligament and the number of osteoblasts in the tension site among the groups (p<0.05). Conclusion: HBO therapy at 2.4 ATA for 7 days is effective in recovery of periodontal ligament and increased osteoblast number during bone remodeling in tension area of orthodontic tooth movement.
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Pinheiro, Gabriela Veloso Vieira da Silva, Robinson Sabino-Silva, Melissa Rodrigues de Araujo, Shaiene Patrícia Gomes, Stephanie Wutke Oliveira, Emília Maria Gomes Aguiar, Léia Cardoso-Sousa, Carla Castiglia Gonzaga und Marcela Claudino. „Experimental Acute Sepsis Reduced Number of Osteocalcin Immunolabeled Cells in Periodontal Ligament“. Brazilian Dental Journal 31, Nr. 2 (April 2020): 143–51. http://dx.doi.org/10.1590/0103-6440202003024.

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Abstract The aim of this study was to evaluate the effect of acute sepsis in the periodontal ligament, alveolar and furcation bone in absence of periodontitis induction through histological and immunohistochemical analyses. A septic rat model was established by cecal ligation and puncture (CLP). Twelve rats were randomly divided into CLP (n=6) and Sham (n=6) groups. The animals were euthanized at 24 h and hemimandibles were submitted to histomorfometric (bone matrix, collagenous fibers, fibroblasts, osteocytes, inflammatory cells, and blood vessels) and immunohistochemical (BMP-2/4, RANKL and osteocalcin) evaluation in alveolar bone, furcation bone and periodontal ligament. Our results demonstrated that histomorphometric parameters were similar in alveolar bone, furcation bone and periodontal ligament of Sham and CLP rats. Regarding to immunohistochemical analyses, the number of BMP-2/4 and RANKL immunolabeled cells was also similar in both groups. Furthermore, it was detected a reduction in the osteocalcin immunolabeled cells in periodontal ligaments of CLP compared to Sham rats (p=0.0014). In conclusion, the acute sepsis induction resulted in reduced number of osteocalcin labelled cells in periodontal ligament region. Moreover, no significant histological differences were observed in the periodontium of rats under acute sepsis. Considering the role of osteocalcin in bone remodeling, the study contributes to revealing the importance of careful periodontal evaluation in the presence of sepsis.
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Khertek, M. V., und S. V. Logvinov. „Morfoquantitative and ultrastructural analysis of blood and lymph vessels in the first molars and premolars periodontal ligament“. Bulletin of Siberian Medicine 10, Nr. 6 (28.12.2011): 57–60. http://dx.doi.org/10.20538/1682-0363-2011-6-57-60.

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Morfoquantitative and ultrastructural analysis of the specific volumes of blood and lymph vessels in the periodontal first molars and premolars with different surfaces (medial, distal, buccal, and palatal) and at different levels of the roots are studied. It was noted that at physiological pressure on the molars and premolars, the specific volume of the investigated values are different. Throughout the root of the observed two zones of compression and expansion of periodontal. Depending on these areas of blood and lymphatic vessels was significantly different.
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Hasegawa, Tomoka, Yukina Miyamoto-Takasaki, Miki Abe, Zixuan Qiu, Tomomaya Yamamoto, Yimin, Taiji Yoshida et al. „Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats“. Microscopy 68, Nr. 5 (03.07.2019): 349–58. http://dx.doi.org/10.1093/jmicro/dfz021.

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Abstract In this study, we aimed to clarify the role of ascorbic acid in collagen synthesis in periodontal ligaments using osteogenic disorder Shionogi (ODS)/ShiJcl-od/od rats lacking L-gulonolactone oxidase. These rats cannot synthesize ascorbic acid in vivo. Eight-week-old ODS/ShiJcl-od/od male rats were administered ascorbic acid solution at a concentration of 200 mg/dL (control group, n = 6) or ascorbic acid solution at concentration of 0.3 mg/dL (insufficient group, n = 12). Six rats of the insufficient group were then given with ascorbic acid solution at concentration of 200 mg/dL for additional 3 weeks (rescued group, n = 6), and then, their mandibles were histochemically examined. Consequently, the insufficient group specimens were seen to possess fewer collagen fibers, and silver impregnation revealed numerous fine, reticular fiber-like fibrils branching off from collagen in the periodontal ligaments. In control group, faint immunoreactivities for matrix metalloproteinase (MMP)2 and cathepsin H were seen in the periphery of blood vessels and throughout the ligament, respectively. In contrast, in the insufficient group, intense MMP2-immunoreactivity was observed to be associated with collagen fibrils in the periodontal ligaments, and cathepsin H-immunopositivity was seen in ligamentous cells. The rescued group showed abundant collagen fibers filling the periodontal ligament space. Under transmission electron microscopy, ligamentous fibroblasts incorporated collagen fibrils into tubular endosomes/lysosomes while simultaneously synthesizing collagen fibril bundles. Thus, ascorbic acid insufficiency affected the immunolocalization of cathepsin H and MMP2; however, ligamentous fibroblasts appear to possess the potential to synthesize collagen fibers when supplied with ascorbic acid.
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Al-Maula, Bushra Habeeb, Zena Jehad Wally, Mohanad Jameel Najm Al-Magsoosi, Rasha Hatem Dosh, Ruba M. Mustafa, Suhad Jabbar Hamed Al-Nasrawi, Abdullatif Alfutimie und Julfikar Haider. „Studying Effects of Calcium Oxide Nanoparticles on Dentinogenesis in Male Wistar Rats“. International Journal of Dentistry 2021 (24.07.2021): 1–9. http://dx.doi.org/10.1155/2021/9983538.

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This study aimed to evaluate potential impacts of calcium oxide nanoparticles (CaO-NPs) at different dosages on predentin thickness, number of blood vessels, periodontal ligament thickness, and blood glucose level of Wistar rats. Twelve rats were randomly gathered into four groups, untreated (control) and CaO-NP-treated groups at three concentrations (25, 50, and 100 mg/kg of the body weight) over a period of 60 days. Histological investigation was performed on twenty-four lower incisor teeth extracted from all the tested groups under a light microscope, and an automatic Fujifilm was used to measure the blood glucose level. The results showed that regular nanoparticle treatment significantly increased predentin and periodontal ligament thicknesses, a gradual decrease in vascularization in the pulp tissue, and an increase in the blood glucose level as the dosages of nanoparticles administered to the rats increased. Administration of the CaO-NPs at low dosage (25 mg/kg) could be beneficial for the growth and integrity of teeth and dentinal tissues in rats.
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Karakida, Lilian Mary, Cristiano Miranda de Araujo, Aline Cristina Batista Rodrigues Johann, Elisa Souza Camargo, Orlando Motohiro Tanaka und Odilon Guariza Guariza Filho. „Interaction of Anabolic Androgenic Steroids and Induced Tooth Movement in Rats“. Brazilian Dental Journal 28, Nr. 4 (August 2017): 504–10. http://dx.doi.org/10.1590/0103-6440201601119.

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Abstract This study evaluated the interaction between tooth movement and two anabolic androgenic steroids (AAS), Deposteron® and Nebido®. One hundred Wistar rats were divided into 3 groups: control (C) n=30, Nebido experimental (N) n=35 and Deposteron experimental (D) n=35. The control group was subdivided into 6 subgroups: 1, 2, 3, 5, 7 and 14. The experimental groups were subdivided into 7 subgroups: 0, 1, 2, 3, 5, 7 and 14, which corresponded to the day of animal’s euthanasia after applying orthodontic force. Orthodontic devices were used to induce tooth movement using 50 cN of reciprocal force between the maxillary right first molar and the maxillary incisors. After euthanasia, the tissues were processed and stained with hematoxylin and eosin (HE) and tartrate-resistant acid phosphatase (TRAP). Osteoclasts, Howship’s lacunae and blood vessels were quantified. Groups N and D showed acceleration in the reorganization of the periodontal ligament compared to group C. The peak of the histological events occurred in group C on day 5 and in groups N and D on day 3 after installation of the orthodontic device. There was a statistically significant difference in the number of osteoclasts (p<0.05) between groups N3 and C3, and between groups N3 and D3. Supra-physiological doses of the AAS Nebido® and Deposteron® altered the number of osteoclasts, Howship’s lacunae and blood vessels, accelerating the reorganization of the periodontal ligament, resulting in accelerated biological effects from the induced tooth movement in rats.
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Zanoni, Jacqueline N., Nathalia M. Lucas, Aline R. Trevizan und Ivan D. S. Souza. „Histological evaluation of the periodontal ligament from aged wistar rats supplemented with ascorbic acid“. Anais da Academia Brasileira de Ciências 85, Nr. 1 (01.03.2013): 327–35. http://dx.doi.org/10.1590/s0001-37652013005000003.

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Ascorbic acid (AA) is able to neutralize reactive oxygen species and is essential for collagen synthesis. In aging process oxidative stress is elevated. This study aims to investigate the effects of AA supplementation on the periodontal ligament (PL) of rats during aging. Twenty five rats were used and divided into groups: J90 (90-day-old control), E345 (345-day-old control), E428 (428-day-old control), EA345 (345-day-old supplemented with AA from 90-day-old on) and EA428 (428-day-old supplemented with AA from 90-day-old on). We analyzed the thickness, density of fibroblasts and blood vessels and collagen fibers types in the PL. In group J90 there was predominantly type III collagen fibers (87.64%). In animals supplemented with AA, the area filled by type I fibers (group EA345: 65.67%, group EA428: 52.23%) was higher than type III fibers. PL in group EA428 was thicker than the one observed in group E428 (P < 0.05). During natural aging process, AA promoted the maturation of collagen fibers and enhanced angiogenesis in periodontal ligament. One can conclude that the supplementation with AA represented a beneficial factor for the development of PL in aged rats.
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Imber, Jean-Claude, Andrea Roccuzzo, Alexandra Stähli, Nikola Saulacic, James Deschner, Anton Sculean und Dieter Daniel Bosshardt. „Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold“. International Journal of Molecular Sciences 22, Nr. 20 (09.10.2021): 10915. http://dx.doi.org/10.3390/ijms222010915.

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(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, p = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (p = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects.
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Imamura, Kentaro, Yusuke Hamada, Wataru Yoshida, Tasuku Murakami, Saki Nakane-Koyachi, Kouki Yoshikawa und Atsushi Saito. „Investigating the Effects of Dehydrated Human Amnion-Chorion Membrane on Periodontal Healing“. Biomolecules 12, Nr. 6 (20.06.2022): 857. http://dx.doi.org/10.3390/biom12060857.

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Each growth factor (GF) has different effects and targets, and plays a critical role in periodontal healing. Dehydrated human amnion-chorion membrane (dHACM) contains various GFs and has been used to enhance wound healing. The purpose of this study was to evaluate the effects of dHACM on periodontal healing, using in vitro and in vivo experimental approaches. Standardized periodontal defects were created in rats. The defects were randomly divided into three groups: Unfilled, filled with hydroxypropyl cellulose (HPC), and dHACM+HPC. At 2 and 4 weeks postoperatively, periodontal healing was analyzed by microcomputed tomography (micro-CT), and histological and immunohistochemical analyses. In vitro, periodontal ligament-derived cells (PDLCs) isolated from rat incisors were incubated with dHACM extract. Cell proliferation and migration were evaluated by WST-1 and wound healing assay. In vivo, micro-CT examination at 2 weeks revealed enhanced formation of new bone in the dHACM+HPC group. At 4 weeks, the proportions of vascular endothelial growth factor (VEGF)-positive cells and α-smooth muscle actin (α-SMA)-positive blood vessels in the dHACM+HPC group were significantly greater than those in the Unfilled group. In vitro, dHACM extracts at 100 µg/mL significantly increased cell proliferation and migration compared with control. These findings suggest that GFs contained in dHACM promote proliferation and migration of PDLCs and angiogenesis, which lead to enhanced periodontal healing.
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Parlange, L. M., und M. R. Sims. „A T.E.M. stereological analysis of blood vessels and nerves in marmoset periodontal ligament following endodontics and magnetic incisor extrusion“. European Journal of Orthodontics 15, Nr. 1 (01.02.1993): 33–44. http://dx.doi.org/10.1093/ejo/15.1.33.

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Dissertationen zum Thema "Periodontal ligament Blood-vessels"

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Lew, Kenneth. „Microvascular changes in the rat molar periodontal ligament incident to orthodontic tooth extrusion : with special reference to fenestrae“. Title page, contents and summary only, 1986. http://web4.library.adelaide.edu.au/theses/09DM/09dml669.pdf.

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Parlange, Louise Maureen. „A T.E.M. stereological analysis of blood vessels and nerves in marmoset periodontal ligament following orthodontic extrusion /“. Title page, table of contents and summary only, 1991. http://web4.library.adelaide.edu.au/theses/09DM/09dmp252.pdf.

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Foong, Kelvin W. C. „A transmission electron microscope stereological analysis of the blood vessels and nerves in the human mandibular premolar periodontal ligament /“. Title page, contents and summary only, 1994. http://web4.library.adelaide.edu.au/theses/09DM/09dmf686.pdf.

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Kat, Petrina Sai Peng. „Distribution of the epithelial rests of malassez and their relationship to blood vessels of the periodontal ligament during rat tooth development“. Title page, table of contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09DM/09dmk19.pdf.

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Bibliography: leaves 107-117. Describes research which set out to study the distribution of the epithelial rests of Malassez (ERM) and blood vessels in the peridontal ligament (PDL) of the first molar root in developing rat teeth. ERM were originally thought to be fuctionless but are now believed to not only play a role in dental cyst formation, but may also protect against root resorption.
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Chintavalakorn, Suthichai. „T.E.M. stereology of microvascular and neural reconstitution in marmoset incisor periodontal ligament following incisor decoronation, endodontic therapy, orthodontic extrusion, and long term retention“. Thesis, 1994. http://hdl.handle.net/2440/110340.

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On the basis of the animal model, a retention period of between 1 and 3 years in human terms is suggested in order to maximise reconstitution of both vascular and neural systems
Thesis (M.D.S.) -- University of Adelaide, Dept. of Dentistry, 1995?
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Parlange, Louise Maureen. „A T.E.M. stereological analysis of blood vessels and nerves in marmoset periodontal ligament following orthodontic extrusion“. Thesis, 1991. http://hdl.handle.net/2440/127458.

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Kat, Petrina Sai Peng. „Distribution of the epithelial rests of malassez and their relationship to blood vessels of the periodontal ligament during rat tooth development“. Thesis, 2001. http://hdl.handle.net/2440/122247.

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Describes research which set out to study the distribution of the epithelial rests of Malassez (ERM) and blood vessels in the peridontal ligament (PDL) of the first molar root in developing rat teeth. ERM were originally thought to be fuctionless but are now believed to not only play a role in dental cyst formation, but may also protect against root resorption.
Thesis (M.D.S.) -- University of Adelaide, Dental School, 2001.
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