Zeitschriftenartikel zum Thema „Orchids – Western Australia“

Few plants are so cryptic as the underground orchids, Rhizanthella Rogers (1928: 1), of Australia. Unlike the species on the eastern seaboard of Australia, the Western Australian species spend their entire life cycle, including flowering, below the soil surface (only rarely with the tips of the bracts showing), making them unique among orchids and indeed, among flowering plants generally (Brown et al. 2013). Discovery in 1928 of the first underground orchid in Western Australia was an international sensation where the plant was described as ‘a remarkable subterranean orchid’ (Wilson 1929). The new taxon described in this paper resolves the enigmatic, disjunct distribution of Rhizanthella in Western Australia, where there was thought to be a central and southern node of a single species, R. gardneri Rogers (1928: 1).

Twenty-three orchid species were recorded in Alcoa's permanent vegetation-monitoring plots in unmined and rehabilitated jarrah forest. Of these, 22 were identified in the unmined jarrah forest and 20 were recorded in rehabilitated areas of between 1 and 31 years old. Three species (Cyrtostylis ovata, Lyperanthus serratus and Prasophyllum elatum) were only recorded in the unmined forest and one species was only recorded in rehabilitated areas (Diuris carinata). The overall density of native orchids in the forest was 13 755 plants ha–1, 10 times greater than the density in rehabilitated areas (1381 plants ha–1). The most abundant species in the forest were Cyrtostylis robusta, Caladenia flava, Pterostylis nana and Thelymitra crinita, all with densities greater than 1000 plants ha–1. The most abundant species in the rehabilitated areas were Microtis media, Disa bracteata (an introduced species), Caladenia flava, Pterostylis nana, Diuris longifolia and Pterostylis vittata, all with densities greater than 60 plants ha–1. In rehabilitation older than 10 years, the density of orchids increased to 2685 plants ha–1. Burning in rehabilitated areas resulted in large increases in orchid densities. It is believed that orchid colonisation of rehabilitated bauxite mines is dependent on symbiotic mycorrhiza, which are in turn dependent on development of an organic litter component in the soil.

Recent data on monocotyledonous geophytes from south-western Australia are compiled and compared with those from other areas of mainly mediterranean climate, especially California, Chile and Victoria, Australia. South-western Australia has a high monocot geophyte diversity of 496 species (7% of an estimated native flora of 7100 vascular species), like Victoria (12%) and the Cape region (14%). As in Victoria, orchids are by far the most important group, with c. 400 species, including those likely to be described once ongoing taxonomic research is completed. South-western Australia has higher geophyte numbers than Victoria in all families considered, but a lower geophyte percentage because of a much higher vascular species total. Among south-western Australian non-orchid geophytes, as in Victoria, the most common storage organ is tuberous roots, followed by corms then bulbs and rhizomes, whereas in California bulbs are by far the most common. The presence of microgeophytes with seed-like storage organs is a special feature seen in several phylogenetically unrelated lineages in the south-western Australian and Victorian geophytic flora, especially on granite outcrops. Divergent phylogenetic history is undoubtedly a major factor underlying striking differences between the monocot geophytes of mediterranean Australia, California, Chile, South Africa and Mediterranean countries. Further studies, particularly on the last two regions, will enable better elucidation of these patterns.

This review summarises scientific knowledge concerning the mycorrhizal associations, pollination, demographics, genetics and evolution of Australian terrestrial orchids relevant to conservation. The orchid family is highly diverse in Western Australia (WA), with over 400 recognised taxa of which 76 are Declared Rare or Priority Flora. Major threats to rare orchids in WA include habitat loss, salinity, feral animals and drought. These threats require science-based recovery actions resulting from collaborations between universities, government agencies and community groups. Fungal identification by DNA-based methods in combination with compatibility testing by germination assays has revealed a complex picture of orchid–fungus diversity and specificity. The majority of rare and common WA orchids studied have highly specific mycorrhizal associations with fungi in the Rhizoctonia alliance, but some associate with a wider diversity of fungi. These fungi may be a key factor influencing the distribution of orchids and their presence can be tested by orchid seed bait bioassays. These bioassays show that mycorrhizal fungi are concentrated in coarse organic matter that may be depleted in some habitats (e.g. by frequent fire). Mycorrhizal fungi also allow efficient propagation of terrestrial orchids for reintroduction into natural habitats and for bioassays to test habitat quality. Four categories of WA orchids are defined by the following pollination strategies: (i) nectar-producing flowers with diverse pollinators, (ii) non-rewarding flowers that mimic other plants, (iii) winter-flowering orchids that attract fungus-feeding insects and (iv) sexually deceptive orchids with relatively specific pollinators. An exceptionally high proportion of WA orchids have specific insect pollinators. Bioassays testing orchid-pollinator specificity can define habitats and separate closely related species. Other research has revealed the chemical basis for insect attraction to orchids and the ecological consequences of deceptive pollination. Genetic studies have revealed that the structure of orchid populations is influenced by pollination, seed dispersal, reproductive isolation and hybridisation. Long-term demographic studies determine the viability of orchid populations, estimate rates of transition between seedling, flowering, non-flowering and dormant states and reveal factors, such as grazing and competition, that result in declining populations. It is difficult to define potential new habitats for rare orchids because of their specific relationships with fungi and insects. An understanding of all three dimensions of orchid habitat requirements can be provided by bioassays with seed baits for fungi, flowers for insects and transplanted seedlings for orchid demography. The majority of both rare and common WA orchids have highly specific associations with pollinating insects and mycorrhizal fungi, suggesting that evolution has favoured increasing specificity in these relationships in the ancient landscapes of WA.

Vital-statistics data concerning population viability were gathered for four of the rarest orchids in Western Australia using surveys to define population sizes and habitat areas and annual measurements of plant demographics. These orchids were Caladenia melanema, C. graniticola, C. williamsiae and Drakaea isolata from the wheatbelt of Western Australia. This agricultural area has a Mediterranean climate with unreliable rainfall, and is >80% cleared of native vegetation. Surveys with 10–30 volunteers increased population-size estimates by up to 10 times and provided spatial data to define core habitat areas. These areas included most of the individuals of a species, but were only 2–10 ha in size. Within these areas, orchids were often highly aggregated in patches a few metres wide, potentially resulting in a high degree of intraspecific competition. Vital statistics were obtained using 4-m wide and 30–50-m-long transects to measure rates of emergence, flowering, grazing and seed-set for each orchid. Plants emerging at the same position in different years were considered to be the same individual, but most emerged in new positions. Many plants emerged just once in 4 years, and 2–3 years of dormancy was common. Emergence frequencies were used to provide estimates of population sizes that were two or three times larger than suggested by data from a single year. Seed production was typically very low. Grazing by kangaroos and rabbits was most severe for C. melanema, but was greatly reduced by fencing. Severe drought prevented flowering of C. graniticola in the driest year, whereas other species were more resilient. These orchids are likely to persist as long as there are some years where rainfall is sufficient for flowering and seed set followed by a year with adequate rain for seed germination. Populations of all these orchids were stable or increasing, but they are still at high risk of extinction because of the impacts of increasing soil salinity or fire on their habitats. These species are unlikely to spread elsewhere in the highly cleared and fragmented wheatbelt. Intervention by hand-pollination, grazing protection and translocation to new locations is required to mitigate these risks. Results were summarised in vital statistics report cards with thresholds set to inform conservation management for these species. Core habitat maps and vital-statistics report cards should also be valuable new tools for terrestrial-orchid conservation in other biomes.

The pollination of 20 common terrestrial orchids was studied in a 60-ha urban banksia and eucalypt dominated woodland in Western Australia. Five years of data (24,000 flowers, 6800 plants) measured fruit set relative to floral areas, capsule volumes, climate, phenology, pollination mechanisms, disturbance tolerance and demography. Pollination varied from 0–95% of flowers, floral displays from 90–3300 mm2 and capsules from 15–1300 mm3 per spike. Pollination traits strongly influenced outcomes, with self-pollination highest (59—95%), followed by sexually deceptive autumn or winter-flowering (18–39%), visual deception (0–48%) and sexually deceptive spring-flowering (13–16%). Pollination was limited by drought in autumn or spring and cool winter temperatures. Some orchids were resilient to drought and one formed seed after the leaves withered. Plant density had the greatest impact on fruit set for orchids forming large groups, especially for sexually deceptive pollination. Consequently, small group average (SGA) pollination was up to 4× greater than overall averages and peak seed production occurred in the best locations for genetic exchange and dispersal. SGA rates and seedpod volumes were strongly linked to clonality, but not to demographic trends. Resource competition limited flowering at higher plant densities and competition within spikes resulted in smaller, later-forming seedpods. Pollination data from co-occurring common orchids identified five evolutionary trade-offs linked to pollination, provided baseline data for rare species and revealed impacts of changing climate.

Data on monocotyledonous geophytes from a recent Victorian flora are compiled and compared with those from California and some other areas of mainly mediterranean climate. Victoria's monocot geophyte diversity of 9% places it with parts of South Africa and Western Australia in a group of much higher diversity than California and Chile. The Victorian list is dominated by orchids (all with tuberous roots) and that from California by Alliaceae, Calochortaceae and Liliaceae, with bulbs being the predominant storage organ. Only four families of the 17 involved have native species in both California and Victoria. Most taxa in both areas are dormant in summer and grow during the cool season. However, the Amaryllidaceae found in the Sonoran Desert and the driest parts of Victoria are able to grow in the warm season in response to summer rain.

Orchids require mycorrhizal fungi for germination of seed and growth of seedlings. The colonisation of bauxite-mine rehabilitation areas by orchids is therefore dependent on the availability of both seed and mycorrhizal fungi. Orchid mycorrhizal fungi baiting trials were carried out in rehabilitation areas that were 1, 10 and 26 years old (established in 2001, 1992 and 1976) and adjacent unmined jarrah forest areas at Jarrahdale, Western Australia. Fungal baits consisted of buried six-chambered nylon-mesh packets containing seed of six jarrah forest orchid taxa, Caladenia flava subsp. flava R.Br., Disa bracteata Sw., Microtis media subsp. media R.Br., Pterostylis recurva Benth., Pyrorchis nigricans (R.Br.) D.L.Jones & M.A.Clem. and Thelymitra crinita Lindl. Detection of orchid mycorrhizal fungi was infrequent, especially at the youngest rehabilitation sites where only mycorrhizal fungi associated with P. recurva were detected. Mycorrhizal fungi of the other orchid taxa were widespread but sparsely distributed in older rehabilitation and forest areas. Detection of mycorrhizal fungi varied between taxa and baiting sites for the two survey years (2002 and 2004). Caladenia flava subsp. flava and T. crinita mycorrhizal fungi were the most frequently detected. The presence of C. flava mycorrhizal fungi was correlated with leafy litter cover and maximum depth, and soil moisture at the vegetation type scale (50 × 5 m belt transects), as well as tree and litter cover at the microhabitat scale (1-m2 quadrats). The presence of T. crinita mycorrhizal fungi was positively correlated with soil moisture in rehabilitation areas and low shrub cover in forest. The frequency of detection of orchid mycorrhizal fungi both at rehabilitated sites (15–25% of baits) and in unmined forest (15–50% of baits) tended to increase with rehabilitation age as vegetation recovered. The failure of some orchid taxa to reinvade rehabilitation areas is unlikely to be entirely due to absence of the appropriate mycorrhizal fungi. However, since the infrequent detection of fungi suggests that they occur in isolated patches of soil, the majority of dispersed orchid seeds are likely to perish, especially in recently disturbed habitats.

Orchids are generally regarded as plants with an insignificant invasive potential and so far only one species has proved to be harmful for native flora. However, previous studies on Epipactis helleborine and Arundina graminifolia indicate that the ecological aspects of range extension in their non-native geographical range are not the same for all species of orchids. Disa bracteata in its native range, South Africa, is categorized as of little concern in terms of conservation whereas in Australia it is naturalized and considered to be an environmental weed. The aim of this research was to determine the ecological preferences enabling the spread of Disa bracteata in Western and South Australia, Victoria and Tasmania and to evaluate the effect of future climate change on its potential range. The ecological niche modeling approach indicates that most of the accessible areas are already occupied by this species but future expansion will continue based on four climate change scenarios (rcp26, rcp45, rcp60, rcp85). Further expansion is predicted especially in eastern Australia and eastern Tasmania. Moreover, there are some unpopulated but suitable habitats in New Zealand, which according to climate change scenarios will become even more suitable in the future. The most striking result of this study is the significant difference between the environmental conditions recorded in the areas which D. bracteata naturally inhabits and invasive sites—that indicates a possible niche shift. In Australia the studied species continues to populate a new niche or exploit habitats that are only moderately represented in South Africa.

Drakaea Lindley, 1840 is a genus of 10 species of geophytic orchids endemic to the South-west Australian Floristic Region. The genus is renowned for its morphological and chemical adaptations, achieving pollination by sexual deception of male thynnid wasps. The history of taxa in Drakaea has been one of dispute and confusion right to the present day. Here we provide a revision of the genus, the first made by using modern collections and field data, formalising names for undescribed taxa featured by Hoffman and Brown (1992, 1998), several of which are threatened with extinction. We describe six new species: D. andrewsiae, D. concolor, D. confluens, D. gracilis, D. isolata and D. micrantha. Experimental baiting of male wasps has helped show the specific status of some of these new taxa. Molecular phylogenetic research is needed to clarify relationships and patterns of speciation in the genus. Five of the 10 Drakaea species are legally protected under the Western Australian Wildlife Conservation Act and the Commonwealth Environment Protection and Biodiversity Conservation Act, signalling the ongoing need for research and management to ensure the conservation of this unique part of Australia’s orchid heritage. D. andrewsiae has been recorded only three times from the Gnowangerup–Tunney district. Urgent surveys are needed to establish its conservation status.

Dormancy is common in many terrestrial orchids in southern Australia and other temperate environments. The difficulty for conservation and management when considering dormancy is ascertaining whether non-emergent plants are dormant or dead. Here we use a multi-state capture–recapture method, undertaken over several seasons, to determine the likelihood of a plant becoming dormant or dying following its annual emergent period and evaluate the frequency of the length of dormancy. We assess the transition probabilities from time series of varying lengths for the following nine terrestrial orchids in the genus Caladenia: C. amoena, C. argocalla, C. clavigera, C. elegans, C. graniticola, C. macroclavia, C. oenochila, C. rosella and C. valida from Victoria, South Australia and Western Australia. We used a Bayesian approach for estimating survivorship, dormancy and the likelihood of death from capture–recapture data. Considering all species together, the probability of surviving from one year to the next was ~86%, whereas the likelihood of observing an individual above ground in two consecutive years was ~74%. All species showed dormancy of predominantly 1 year, whereas dormancy of three or more years was extremely rare (<2%). The results have practical implications for conservation, in that (1) population sizes of Caladenia species are more easily estimated by being able to distinguish the likelihood of an unseen individual being dormant or dead, (2) population dynamics of individuals can be evaluated by using a 1–3-year dormancy period and (3) survey effort is not wasted on monitoring individuals that have not emerged for many years.

Two new sympatric species of Caladenia from relatively inaccessible, high lateritic plateaux in a high rainfall area in southwestern Western Australia are described here as new to science. The species occur in a restricted area, c 100 km southeast of Perth, south of Dwellingup and northeast of Waroona. The two species co-occur and are associated with putative floral model species that they are mimicking. Caladenia lateritica mimics Conostylis setosa (Haemodoraceae) in terms of flowering time, height, colour and fragrance. Caladenia rosea mimics the flowers of Hypocalymma robustum (Myrtaceae) in terms of flowering time, colour and scent. We assume that these orchids share a pollinator with their mimics and attract the pollinator via food deception as the ‘hosts’ are sources of nectar and pollen for visiting native bees. Because the species co-occur and exhibit a remarkable evolutionary ‘arms race’, they would make an ideal model for further study of the processes driving sympatric speciation. Unfortunately, both new species are under threat from mining activities and poorly informed burning regimes.

Olives (Olea europaea ssp. europaea), dispersed from 19th century orchards in the Adelaide area, have become established in remnant bushland as a major environmental weed. Recent expansion of the Australian olive industry has resulted in the widespread planting of olive orchards in South Australia, Victoria, New South Wales, Western Australia, Queensland and parts of Tasmania. This paper reviews the literature on the activity of vertebrate (principally avian) olive predators and their potential as vectors for spreading this plant into Australian remnant bushland. The effects of feralisation on the olive plant, which enhances its capacity for dispersal as a weed, place wider areas of south-eastern Australia at risk. A number of approaches for the control of olives as woody weeds are addressed. Proponents of new agricultural crops have moral and environmental obligations to assess the weed potential of these crops.

A method is described for the in vitro propagation of the Australian terrestrial orchid Diuris longifolia R. Br. Explants from inflorescences of D. longifolia were successfully cultured on modified Burgeffs N3f containing the cytokinin N6 benzyladenine. Protocorm-like bodies formed after 49 days on explants taken from the basal section of flower buds and axillary nodes from inflorescences. Root formation occurred 70 days after transfer of 10-20-rnm-long shoots onto medium containing coconut water and no cytokinin, and rooted plantlets were transferred to soil and acclimatised 2-3 weeks later. Rooting of in vitro shoots was improved by increasing the sucrose concentration to 40 g/L or by addition of 0.05% activated charcoal to the culture medium. Plantlets developed flowering stems in vitro and flowered in pots 11 months after excision of explants from the parent plant. This tissue culture method has been successfully applied to the propagation of D. purdiei, a rare and threatened species from Western Australia.

The impact of seed drying, seed storage and development of testing procedures for seed viability assessment was undertaken for a selection of common taxa with congeners that are rare and endangered (Caladenia, Diuris, Pterostylisand Thelymitra). Freshly collected seed showed significantly lower levels of germination compared with seed that had been subjected to drying over silica gel for 24 h. Seed dried over silica gel for 24 h and plunged into liquid nitrogen exhibited a further increase in germination levels. Germination of seed stored at 4, 18 or 22˚C for 1 year was substantially higher than freshly collected seed (4 weeks after dehiscence), but germination was highest overall after storage of dried seed in liquid nitrogen (–196˚C). Mycorrhizal fungi that promote the germination and growth of plants were also successfully preserved in liquid nitrogen. The use of cryoprotectants on fungal isolates had no observable deleterious effects on fungal regeneration. Histochemical staining procedures (tetrazolium, fluorescein diacetate and Evans blue) substantially overestimated seed viability, relative to symbiotic seed germination, for most seed treatments indicating a need for re-evaluation of the effectiveness of staining procedures for testing viability. The implications of the long-term ex situ storage of orchid seed and fungal symbionts for the conservation of endangered orchids is discussed.

The Western Australian members of the broombush complex, Melaleuca uncinata R.Br., are revised. Eleven species of the complex are recognised, of which seven are newly described: M. atroviridis Craven & Lepschi, M.�exuvia Craven & Lepschi, M.�interioris Craven & Lepschi, M.�osullivanii Craven & Lepschi, M.�scalena Craven & Lepschi, M.�vinnula Craven & Lepschi, M.�zeteticorum Craven & Lepschi. An identification key is provided, and the Western Australian distribution of each species is mapped. The occurrence of hybridisation between members of the complex is reported. The broombush species with which the Western Australian underground orchid, Rhizanthella gardneri Rogers (Orchidaceae), is associated are discussed.

The establishment of five species of temperate terrestrial orchids (Caladenia arenicola Hopper & A.P.Brown, Diuris magnifica D.L.Jones, D. micrantha D.L.Jones, Pterostylis sanginea D.LJones & M.A.Clem. and Thelymitra manginiorum ms) in natural habitat through in situ seed sowing, or by planting of seedlings and dormant tubers, was evaluated. Seed of the Western Australian temperate terrestrial taxa, Caladenia arenicola and Pterostylis sanguinea germinated best when sown into soil inoculated with mycorrhizal fungi at field sites but failed to develop the tubers necessary for surviving summer dormancy. However, seedling survival improved when actively growing symbiotic seedlings were transferred to natural habitat during the growing season. Caladenia arenicola and P. sanguinea seedlings survived the initial transfer to field sites but only P. sanguinea survived into the second growing season. Highest survival was obtained by translocating dormant tubers of C. arenicola and Diuris magnifica, with D. magnifica persisting at the site 5 years after translocation. However, outplanted C. arenicola survived for only 2 years. In another trial, where seedlings and dormant tubers of a rare orchid Thelymitra manginiorum were translocated into eucalypt woodland, 18% persisted after 5 years. The rare orchid D. micrantha exhibited the highest survival rates, with greater than 80% of tubers surviving 5 years after transfer of mature dormant tubers to field sites. This study highlights the benefit of using optimised methods for seedling production by symbiotic germination and nursery growth to produce advanced seedlings or dormant tubers to maximise the survival of translocated plants. It also demonstrates the need to consider different strategies when dealing with individual species.

Domestic wastewater reuse is currently not permitted anywhere in Australia but is widely supported by the community, promoted by researchers, and improvised by up to 20% of householders. Its widespread implementation will make an enormous contribution to the sustainability of water resources. Integrated with other strategies in the outdoor living environment of settlements in arid lands, great benefit will be derived. This paper describes six options for wastewater reuse under research by the Remote Area Developments Group (RADG) at Murdoch University and case studies are given where productive use is being made for revegetation and food production strategies at household and community scales. Pollution control techniques, public health precautions and maintenance requirements are described. The special case of remote Aboriginal communities is explained where prototype systems have been installed by RADG to generate windbreaks and orchards. New Australian design standards and draft guidelines for domestic greywater reuse produced by the Western Australian State government agencies for mainstream communities are evaluated. It is recommended that dry composting toilets be coupled with domestic greywater reuse and the various types available in Australia are described. For situations where only the flushing toilet will suffice the unique “wet composting” system can be used and this also is described. A vision for household and community-scale on-site application is presented.

The development of a simple, durable, lightweight and disposable beehive for high-density (and netted) orchards and crops distant from beekeeping areas provides a useful product and a further diversification for beekeepers involved in professional pollination services. The total weight of a fully developed Beetube ranged from 2.23 to 3.64 kg, contained about 9101 bees, 3038 cm2 of comb, and had a morning and afternoon flight activity of 19-43 and 11-34 honey bees per minute, respectively.

This research aimed to improve the success of soil transfer of terrestrial orchid seedlings after symbiotic germination in the laboratory. Three native Western Australian terrestrial orchids (Caladenia arenicola Hopper & A.P.Brown, Diuris magnifica D.L.Jones and Thelymitra crinita Lindley) were used in this study. The key to improved seedling survival on transfer to soil was found to be the use of an intermediate stage between the Petri dish and soil where larger seedlings were grown in an axenic environment with controlled humidity. There was no apparent benefit of pre-inoculating potting medium with appropriate strains of mycorrhizal fungi for subsequent growth of symbiotic seedlings under glasshouse conditions. Initial survival of seedlings in the glasshouse was high. However, some seedlings failed to produce tubers (from modified roots or droppers) necessary for plant survival through the summer dormancy period, and this caused survival to decrease to 40–60% of the glasshouse-grown seedlings in the first year. The initiation of tubers on droppers by C. arenicola was inversely correlated with leaf size, with smaller plants more likely to form tubers. This suggests that leaves and tubers were competing for resources. However, larger seedlings that did tuberise had larger tubers that were more likely to survive summer dormancy. There was no correlation between leaf size and root tuber size in D. magnifica, but the number of tubers produced was greatest in larger plants. As with C. arenicola, plants of D. magnifica and T. crinita with larger tubers were more likely to survive summer dormancy. Methods developed in this study enable the production of both actively growing symbiotic seedlings and dormant tubers which improve the success of translocation of laboratory-grown terrestrial orchids to field sites.

Rhizanthella gardneri R.S.Rogers is an entirely subterranean mycoheterotrophic orchid known only from two isolated populations within south-western Western Australia (WA). This rare species appears restricted to habitats dominated by species of the Melaleuca uncinata complex. R. gardneri purportedly forms a tripartite relationship with Melaleuca1, via a connecting mycorrhizal fungus, for the purpose of carbohydrate and nutrient acquisition. Here, we quantify key climate, soil and vegetation characteristics of known R. gardneri habitats to provide baseline data for monitoring of known R. gardneri populations, to better understand how R. gardneri interacts with its habitat and to identify possible new sites for R. gardneri introduction. We found that the habitats of the two known R. gardneri populations show considerable differences in soil chemistry, Melaleuca structure and Melaleuca productivity. Multivariate analyses showed that both multidimensional scaling (MDS) and principal components analysis (PCA) ordinations of soil chemical characteristics were very similar. Individual sites within populations were relatively similar in all attributes measured, whereas overall northern and southern habitats were distinct from each other. These results suggest that R. gardneri can tolerate a range of conditions and may be more widespread than previously thought, given that there are extensive areas of Melaleuca thickets with similar habitat characteristics across south-western WA. Variability within the habitats of known R. gardneri populations suggests translocation of this species into sites with similar vegetation may be a viable option for the survival of this species.

Damage to 6 orchards of apples, pears, plums and nectarines in Western Australia by the parrots Purpureicephalus spurius, Platycercus icterotis and Barnardius zonarius was studied in 1973-75 and damage by the cockatoo Calyptorhynchus funereus baudinii was recorded opportunistically for comparison. There was up to 12% damage to individual varieties of fruit, least to green varieties of apple. In any one season no single orchard had losses over 1.4% of total fruit grown and none had net income affected by more than $A100. Damage by those smaller parrots was of little economic significance during the study. The cockatoo caused more damage to apples, and in a shorter time, than did the small parrots.

Five species of introduced earthworm were recorded in the northern jarrah forest of Western Australia during 1980-83. These are Aporrectodea trapezoides (Duges), A. caliginosa (Savigny), Eisenia fetida (Savigny), Octolasion cyaneum (Savigny) (all Lumbricidae) and Microscolex dubius (Fletcher) (Megascolecidae). A. trapezoides was recorded most frequently. These introduced species occur within the forest only where there has been major disturbance, especially where forest has been replaced by pasture, orchards or settlement. They have not been recorded in forest that has been logged or in plantations of exotic trees. Introduced species of earthworm were frequently found in association with indigenous species. Most individuals of A. trapezoides kept in jarrah forest soil in the laboratory lost weight over 30 days, in contrast to an indigenous species of earthworm. How and when earthworm species were introduced is discussed in terms of the early European history of the jarrah forest.

An experiment was conducted at Manjimup Research Station in Western Australia from 1973 to 1982 to determine the optimum rates of fertilizer applications for apple trees (Granny Smith/MM.104 and Hi Early Red Delicious/MM.104) growing in a P-deficient soil (about 6 mg/g bicarbonate-extractable P). The trees were planted at 6.1 multiply 6.1 m spacings. Superphosphate at 5 or 8 kg/tree consistently increased fruit set, but the increase varied from year to year from 8 to 91%. Weight of prunings increased by 24-29% and tree height multiply width by 11-16%. When results were averaged over both cultivars, weight of fruits harvested from 1973 to 1982 was increased from 853 to 1226 kg/tree by superphosphate application at 5 kg/tree. Ammonium nitrate applied at up to 4 kg/tree gave small non-significant increases in growth and no increase in weight of fruits harvested for Granny Smith, but the weight of the Red Delicious crop rose by 11%. No response to potassium chloride at 1 kg/tree was found. P fertilizer application increased P concentration in the first fully mature leaves of Granny Smith trees from approximately 0.14% to 0.17-0.19% and in Red Delicious leaves from 0.17 to 0.20-0.21%. N fertilizer application increased N concentration in Granny Smith leaves from approximately 2.3 to 2.5%. Lack of P was the main limiting factor on growth and cropping and it is suggested that the importance of P nutrition in Western Australian apple orchards has been underestimated.

Diuris is a terrestrial orchid genus of at least 61 and possibly more than 100 species, restricted to Australia except for one species endemic in Timor. Distinctive species groups have respective eastern and western centres of distribution. Although species affinities have been vaguely understood for many years, no formal infrageneric treatment has been undertaken as Diuris possesses few reliable morphological characters for a classification system. We have undertaken cladistic parsimony and Bayesian phylogenetic analyses of Diuris by using the ITS1–5.8S–ITS2 region of nuclear rDNA and morphological characters, with a subset of samples also studied by amplified fragment length polymorphism (AFLP) as an independent test of phylogenetic relationships. Four major clades with strong bootstrap support were resolved and are named here according to a recently published classification; D. sulphurea forms a lineage (subg. Paradiuris) of its own that is well supported as the sister to the rest of Diuris. Two other major eastern clades contained species related to D. maculata (subg. Xanthodiuris) and D. punctata (subg. Diuris), respectively. Although these latter two subgenera are genetically well resolved, there is minimal genetic variation at species level, consistent with recent, rapid speciation. A fourth clade (subg. Hesperodiuris) has a centre of distribution in Western Australia, and has more genetic and morphological variation than the eastern subgenera. Total evidence analysis provides support for the western clade being sister group to the two eastern subgenera Diuris and Xanthodiuris; however, this relationship was not resolved by molecular data. Hybridisation is known to be common among species within subgenera Diuris and Xanthodiuris. Instances of incongruence between different datasets were found suggestive of hybridisation events between species of different sections of Diuris.

Abstract The mating system of Western Australian sandalwood (Santalum spicatum) was investigated in seed collections from a family trial. The mean outcrossing rate was high (95.2%) although there was variation among families and one family showed particularly low outcrossing, high bi-parental inbreeding and high correlated paternity. Variation in flowering time between this family and others in the trial was the most likely cause of the high inbreeding and low number of fathers contributing to the seed crop in trees from this family. The high level of outcrossing in the rest of the families indicated that S. spicatum has a preferentially outcrossed mating system, but that it is capable of selfing when conditions are unsuitable for outcrossing. The seedling progeny showed no influence of inbreeding on seedling height at 4 months. Analysis of genetic diversity revealed high levels of genetic diversity have been captured in the seed crop from the trial, and this diversity is comparable to that identified in a previous study of natural populations from throughout the range of the species. These results indicated that production of seed through seed orchards will be a successful means of deployment of breeding gains in the species, however it is essential to ensure overlap in flowering times among different families planted in a seed orchard.

Low capsule set is a major factor limiting seed production in Eucalyptus globulus seed orchards. Trials were conducted in E. globulus seed orchards in Tasmania, Australia, to identify the timing of capsule development and abortion, as well as the influence of pollination type, the number of ovules fertilised and weather events on capsule set. Controlled pollination (CP), mass supplementary pollination (MSP), open pollination (OP) and isolated unpollinated control (UP) treatments were performed on 21 genotypes in an orchard in southern Tasmania in 2004–2005 and on six genotypes in a higher-altitude orchard in north-western Tasmania in 2005–2006. No capsules were set in the UP control treatment, and capsule set was significantly lower following CP than OP and MSP. The major period of capsule abortion occurred between 20 and 80 days after pollination for all pollination methods across both sites, coinciding with the period of capsule growth. A positive correlation between the number of fertilised ovules per aborted capsule and the length of time capsules were held on the tree was recorded. Given that capsule abortion occurred during a period of fruit growth and that capsules with the lowest number of fertilised ovules aborted first, it is argued that fertilisation level and the level of resource competition are major factors determining capsule abortion.

The macadamia nut was introduced to the Kenyan highlands from Australia in the early 1960s. Seedlings were propagated at a nursery near Kiambu in central Kenya by Bob Harris and were subsequently distributed in the central and eastern highlands and later the western highlands. The majority of seedlings planted were one two species, Macadamia integrifolia Maiden and Betche or M. tetraphylla L. S. A less common species, Macadamia ternifolia, was also planted. Several hybrids of M. integrifolia and M. tetraphylla have been identified in the central and eastern highlands. A macadamia improvement research program was launched in the early part of 1980 by the Ministry of Agriculture. Since then, 30 trees of the seedlings planted in the later part of 1960s have been selected and evaluated in trial orchards located in the Kenyan highlands. Most of the recently planted orchards constitute of 10 clones that yield between 40 to 90 kg of nuts annually. Five high-yielding macadamia varieties from Hawaii were introduced to Kenya in the early 1980s. To date >90% of the cultivated macadamia trees in Kenya are either M. integrifolia or hybrids of M. integrifolia and M. tetraphylla. Until the late 1970s, there was no market for macadamia nuts in Kenya. Since then, several companies market this crop, which is mainly exported to Japan and Europe.

AbstractThe Forest Products Commission of Western Australia manages a sandalwood (Santalum spp.) core germplasm collection at Kununurra in the states far north. This collection serves as a significant seed source for sandalwood plantations in the area and remains an important resource for ongoing research. The collection contains S. album trees sourced from Indian arboreta, along with a few trees from West Timor, Indonesia. Also present are representatives of S. macgregorii from Papua New Guinea and S. austrocaledonicum from Vanuatu and/or New Caledonia. Despite the apparently diverse seed origins, the genetic background of many of the accessions remains vague. In this study, diversity and relatedness was assessed by nuclear and chloroplast RFLPs and a phylogeny was inferred. Nuclear RFLPs revealed very low levels of genetic diversity for a tree species, with an observed and expected heterozygosity (Ho and He) of 0.047. Nineteen genotypes were identified within the 233 S. album individuals sampled, with only one tree known to have originated from Timor being differentiated from Indian material. Other trees thought to have come from Timor grouped with those believed to be from India, indicating they were either incorrectly labelled or sourced from heavily modified populations. Despite the poor sample size, chloroplast RFLP analysis revealed no genetic distinction between the Timorese and Indian S. album, which supports the theory of human mediated seed dispersal from Timor to India. The structure of the phylogeny and associated relatedness has assisted in the establishment of seed orchards, designed to ensure maximum diversity is maintained through limiting the proximity of highly related trees. Finally, in light of these and other findings, a hypothesis concerning the evolution of S. album is proposed.

The chestnut (Castanea Mill.) industry in the northwestern United States is in its relative infancy, with most orchards being less than 10 years of age. Currently there are an estimated 300 acres (121 ha) in Oregon and Washington. California has about 500 acres (202 ha) in chestnuts. Current worldwide production is over 500,000 tons (435,600 t). China is the leading producer with 40%, followed by Korea at 15%. Italy, Turkey and Japan grow 10% each, while France, Greece and Spain grow 4% each. The United States, Chile, Argentina, New Zealand and Australia each grow less than 1%. The value of chestnuts imported into the United States is estimated to be $10 to 15 million annually. Domestic producers hope to displace some of the imported chestnuts in the marketplace. The leading variety being grown in the western United States is `Colossal,' a hybrid between european chestnut (C. sativa Mill.) and japanese chestnut (C. crenata Gillet). `Dunstan' hybrids are chestnut blight (Cryphonectria parasitica Murr.) resistant, and were bred in Florida using chinese chestnut (C. mollisima Blume) and american chestnut (C. dentata Marsh. Borkh.) parentage. Prices received by chestnut producers in the northwestern United States have ranged from $1.20 to $7.00/lb ($2.64 to $15.40/kg). The marketing of chestnuts has been through brokers into wholesale markets, farmers markets, mail order and direct sales through catalogues and World Wide Web sites.

Monilinia fructicola, the causal agent of brown rot, is a destructive fungal pathogen that affects mainly stone fruits (Prunoideae). It causes fruit rot, blossom wilt, twig blight, and canker formation and is common in North and South America, Australia, and New Zealand. M. fructicola is listed as a quarantine pathogen in the European Union and was absent from this region until 2001 when it was detected in France. In August 2009, mature peaches (Prunus persica cv. Royal Glory) with brown rot were found in a 5-year-old orchard in Goriška, western Slovenia. Symptoms included fruit lesions and mummified fruits. Lesions were brown, round, rapidly extending, and covered with abundant gray-to-buff conidial tufts. The pathogen was isolated in pure culture and identified based on morphological and molecular characters. Colonies on potato dextrose agar (PDA) incubated at 25°C in darkness had an average daily growth rate of 7.7 mm. They were initially colorless and later they were light gray with black stromatal plates and dense, hazel sporogenous mycelium. Colony margins were even. Sporulation was abundant and usually developed in distinct concentric zones. Limoniform conidia, produced in branched chains, measured 10.1 to 17.7 μm (mean = 12.1 μm) × 6.2 to 8.6 μm (mean = 7.3 μm) on PDA. Germinating conidia produced single germ tubes whose mean length ranged from 251 to 415 μm. Microconidia were abundant, globose, and 3 μm in diameter. Morphological characters resembled those described for M. fructicola (1). Morphological identification was confirmed by amplifying genomic DNA of isolates with M. fructicola species-specific primers (2–4). Sequence of the internal transcribed spacer (ITS) region (spanning ITS1 and ITS 2 plus 5.8 rDNA) of a representative isolate was generated using primers ITS1 and ITS4 and deposited in GenBank (Accession No. GU967379). BLAST analysis of the 516-bp PCR product revealed 100% identity with several sequences deposited for M. fructicola in NCBI GenBank. Pathogenicity was tested by inoculating five mature surface-sterilized peaches with 10 μl of a conidial suspension (104 conidia ml–1) obtained from one representative isolate. Sterile distilled water was used as a control. Peaches were wounded prior to inoculation. After 5 days of incubation at room temperature and 100% relative humidity, typical brown rot symptoms developed around the inoculation point, while controls showed no symptoms. M. fructicola was reisolated from lesion margins. Peach and nectarine orchards in a 5-km radius from the outbreak site were surveyed in September 2009 and M. fructicola was confirmed on mummified fruits from seven orchards. The pathogen was not detected in orchards from other regions of the country, where only the two endemic species M. laxa and M. fructigena were present. To our knowledge, this is the first report of M. fructicola associated with brown rot of stone fruits in Slovenia. References: (1) L. R. Batra. Page 106 in: World Species of Monilinia (Fungi): Their Ecology, Biosystematics and Control. J. Cramer, Berlin, 1991. (2) M.-J. Côté et al. Plant Dis. 88:1219, 2004. (3) K. J. D. Hughes et al. EPPO Bull. 30:507, 2000. (4) R. Ioos and P. Frey. Eur. J. Plant Pathol. 106:373, 2000.

Capsicum chlorosis virus (CaCV), a thrips-transmitted, tentative species in the genus Tospovirus, family Bunyaviridae, was first identified in solanaceous crops, but also infects several ornamental crops such as orchid (4), gloxinia (3), and calla lily (1). From 2005 to 2007, virus-like yellow ringspots were observed on the leaves of amaryllis (Hippeastrum hybridum Hort.) and blood lily (Haemanthus multiflorus Martyn.) plants cultured in screenhouses and a private garden, respectively. Three of several hundred amaryllis plants in screenhouses from two places were observed as showing yellow ringspot symptoms and one of six blood lily plants was observed as showing similar yellow ringspot symptoms. Sap extracts from symptomatic leaves were inoculated to Chenopodium quinoa Willd. and the resulting local lesions were passaged three successive times to C. quinoa for virus isolation. Using the tospovirus genus-specific primers gL3637 and gL4435c designed from the conserved region in the L RNA (2), DNA fragments of the expected size of 800 bp were amplified by reverse transcription (RT)-PCR from field samples and local lesions from C. quinoa. Extracts from the diseased plants and local lesions of C. quinoa reacted strongly with antiserum against the nucleocapsid (N) protein of CaCV in ELISA and western blotting. To confirm the identity of this virus, we amplified the N gene from three amaryllis and one blood lily source using primer pair WN2328 and WN3534 designed from the S RNA of Watermelon silver mottle virus (1), and these products were cloned and sequenced. The sequence from each virus isolate was determined from three independent clones. The nucleotide and deduced amino acid sequences of N genes for the blood lily isolate (GenBank Accession No. EF101344) and three amaryllis isolates (GenBank Accession Nos. EF101343, EF137177, and FJ185170) had identities greater than 97% with that of a CaCV isolate infecting Capsicum spp. found in Australia (GenBank Accession No. AY036057). Phylogenetic analysis using maximum parsimony showed that these sequences clustered with CaCV. These results show that the virus identified from amaryllis and blood lily that were expressing yellow ringspot symptoms are isolates of CaCV. To our knowledge, this is the first report of CaCV naturally infecting amaryllis and blood lily and it could become an important threat to ornamental production in Taiwan. References: (1) C. C. Chen et al. Plant Dis. 91:1201, 2007. (2) F. H. Chu et al. Phytopathology 91:361, 2001. (3) H. T. Hsu et al. J. Gen. Plant Pathol. 66:167, 2000. (4) Y. X. Zheng et al. Eur. J. Plant Pathol. 120:199, 2008.

Abstract STUDY QUESTION Does activin A contribute to testicular fibrosis under inflammatory conditions? SUMMARY ANSWER Our results show that activin A and key fibrotic proteins are increased in human testicular biopsies with leukocytic infiltrates and impaired spermatogenesis and in murine experimental autoimmune orchitis (EAO) and that activin A stimulates fibrotic responses in peritubular cells (PTCs) and NIH 3T3 fibroblasts. WHAT IS KNOWN ALREADY Fibrosis is a feature of EAO. Activin A, a regulator of fibrosis, was increased in testes of mice with EAO and its expression correlated with severity of the disease. STUDY DESIGN, SIZE, DURATION This is a cross-sectional and longitudinal study of adult mice immunized with testicular homogenate (TH) in adjuvant to induce EAO, collected at 30 (n = 6), 50 (n = 6) and 80 (n = 5) days after first immunization. Age-matched mice injected with adjuvant alone (n = 14) and untreated mice (n = 15) were included as controls. TH-immunized mice with elevated endogenous follistatin, injected with a non-replicative recombinant adeno-associated viral vector carrying a gene cassette of follistatin (rAAV-FST315; n = 3) or vector with an empty cassette (empty vector controls; n = 2) 30 days prior to the first immunization, as well as appropriate adjuvant (n = 2) and untreated (n = 2) controls, were also examined. Human testicular biopsies showing focal inflammatory lesions associated with impaired spermatogenesis (n = 7) were included. Biopsies showing intact spermatogenesis without inflammation, from obstructive azoospermia patients, served as controls (n = 7). Mouse primary PTC and NIH 3T3 fibroblasts were stimulated with activin A and follistatin 288 (FST288) to investigate the effect of activin A on the expression of fibrotic markers. Production of activin A by mouse primary Sertoli cells (SCs) was also investigated. PARTICIPANTS/MATERIALS, SETTING, METHODS Testicular RNA and protein extracts collected from mice at days 30, 50 and 80 after first immunization were used for analysis of fibrotic marker genes and proteins, respectively. Total collagen was assessed by hydroxyproline assay and fibronectin; collagen I, III and IV, α-smooth muscle actin (α-SMA) expression and phosphorylation of suppressor of mothers against decapentaplegic (SMAD) family member 2 were measured by western blot. Immunofluorescence was used to detect fibronectin. Fibronectin (Fn), αSMA (Acta2), collagen I (Col1a2), III (Col3a1) and IV (Col4a1) mRNA in PTC and NIH 3T3 cells treated with activin A and/or FST288 were measured by quantitative RT-PCR (qRT-PCR). Activin A in SC following tumour necrosis factor (TNF) or FST288 stimulation was measured by ELISA. Human testicular biopsies were analysed by qRT-PCR for PTPRC (CD45) and activin A (INHBA), hydroxyproline assay and immunofluorescence. MAIN RESULTS AND THE ROLE OF CHANCE Production of activin A by SC was stimulated by 25 and 50 ng/ml TNF (P < 0.01, P < 0.001, respectively) as compared to untreated cells. INHBA mRNA was increased in human testicular biopsies with leukocytic infiltrates and impaired spermatogenesis, compared with control biopsies (P < 0.05), accompanied by increased total collagen (P < 0.01) and fibronectin deposition. Total testicular collagen (P < 0.0001) and fibronectin protein expression (P < 0.05) were also increased in EAO, and fibronectin expression was correlated with the severity of the disease (r = 0.9028). In animals pre-treated with rAAV-FST315 prior to immunization with TH, protein expression of fibronectin was comparable to control. Stimulation of PTC and NIH 3T3 cells with activin A increased fibronectin mRNA (P < 0.05) and the production of collagen I (P < 0.001; P < 0.01) and fibronectin (P < 0.05). Moreover, activin A also increased collagen IV mRNA (P < 0.05) in PTC, while αSMA mRNA (P < 0.01) and protein (P < 0.0001) were significantly increased by activin A in NIH 3T3 cells. LARGE SCALE DATA N/A. LIMITATIONS, REASONS FOR CAUTION A limited number of human testicular specimens was available for the study. Part of the study was performed in vitro, including NIH 3T3 cells as a surrogate for testicular fibroblasts. WIDER IMPLICATIONS OF THE FINDINGS Resident fibroblasts and PTC may contribute to the progression of testicular fibrosis following inflammation, and activin A is implicated as a key mediator of this process. STUDY FUNDING/COMPETING INTEREST(S) This work was supported by the National Health and Medical Research Council of Australia, the Victorian Government’s Operational Infrastructure Support Program and the International Research Training Group between Justus Liebig University (Giessen) and Monash University (Melbourne) (GRK 1871/1–2) on `Molecular pathogenesis on male reproductive disorders’ funded by the Deutsche Forschungsgemeinschaft and Monash University. The authors declare no competing financial interests.

A detailed study of taxonomic features of the Eriochilus dilatatus (Orchidaceae) complex (white bunny orchids) in Western Australia found that there were no consistent differences among most subspecies when herbarium specimens or living plants were compared. These subspecies were originally segregated primarily by differences in leaf size and shape and the number of flowers produced, but a critical examination of herbarium specimens found that these features were highly inconsistent within taxa. These features were also found to be highly variable over time and space within populations of living plants. The distribution patterns, habitat preferences and flowering times of these taxa were found to overlap, even for subspecies brevifolius and orientalis, which occupy the northern and eastern limits of the distribution of this species. Eriochilus dilatatus subsp. magnus and subsp. multiflorus were shown to be synonyms of subsp. dilatatus, whereas subsp. undulatus and subsp. orientalis are synonymised under subsp. brevifolius. As a result of this study, the two recognised subspecies are subsp. dilatatus and subsp. brevifolius, which can be readily separated by plant height, flower numbers and leaf morphology, except for a few intermediate plants where ranges overlap. New keys and descriptions to these taxa are provided. The reasons for previous taxonomic confusion in this group and in many other Western Australian orchids are discussed and research approaches to resolve these issues are suggested.

Abstract Many orchids are characterized by small, patchily distributed populations. Resolving how they persist is important for understanding the ecology of this hyper-diverse family, many members of which are of conservation concern. Ten populations of the common terrestrial orchid Drakaea glyptodon from south-western Australia were genotyped with ten nuclear and five plastid simple sequence repeat (SSR) markers. Levels and partitioning of genetic variation and effective population sizes (Ne) were estimated. Spatial genetic structure of nuclear diversity, together with plastid data, were used to infer the effective number of seed parents per population. We found high genetic diversity, Ne values that generally exceed predictions based on the number of flowering individuals and moderate levels of gene flow. Two populations were founded by less than five colonists suggesting some populations are colonized by few seeds, with growth largely resulting from in situ recruitment. A value of 3.65 for mp /ms indicates that pollinators play a greater role than seed in introducing genetic diversity to populations via gene flow. Our results highlight that D. glyptodon is highly effective at persisting in patchily distributed populations. However, it is important to examine how insights from this common, widespread species transfer to species that are rare and/or occur in fragmented landscapes.

Abstract A new distribution map is provided for Odontoglossum ringspot virus. Virus: Tobamovirus. Main hosts: Orchidaceae, including Cattleya, Cymbidium, Dendrobium, tiger orchid (Rossioglossum grande) and Vanilla sp. Information is provided on the geographical distribution in Europe (Belgium, Croatia, France, Germany, Hungary, Lithuania, the Netherlands, Poland, UK, Ukraine), Asia (China, Guangdong, Hainan, India, Sikkim, Indonesia, Japan, Honshu, Korea Republic, Malaysia, the Philippines, Singapore, Taiwan, Thailand, Vietnam), Africa (Reunion, South Africa), North America (Canada, British Columbia, Ontario, Quebec, USA, California, Florida, Hawaii, Pennsylvania, Texas, Virginia), Central America and Caribbean (Guadeloupe, Puerto Rico), South America (Brazil, Sao Paulo, Colombia, Venezuela), Oceania (Australia, New South Wales, Northern Territory, Queensland, South Australia, Tasmania, Victoria, Western Australia, Cook Islands, Fiji, French Polynesia, New Zealand, Niue, Tonga, Vanuatu).

Introduction“From every kind of man obedience I expect; I’m the Emperor of Japan.” (“Miyasama,” from Gilbert and Sullivan’s musical The Mikado, 1885)This commentary is facilitated by—surprisingly resilient—oriental stereotypes of an imagined Japan (think of Oscar Wilde’s assertion, in 1889, that Japan was a European invention). During the Victorian era, in Britain, there was a craze for all things oriental, particularly ceramics and “there was a craze for all things Japanese and no middle class drawing room was without its Japanese fan or teapot.“ (V&A Victorian). These pastoral depictions of the ‘oriental life’ included the figures of men and women in oriental garb, with fans, stilt shoes, kimono-like robes, and appropriate headdresses, engaging in garden-based activities, especially tea ceremony variations (Landow). In fact, tea itself, and the idea of a ceremony of serving it, had taken up a central role, even an obsession in middle- and upper-class Victorian life. Similarly, landscapes with wild seas, rugged rocks and stunted pines, wizened monks, pagodas and temples, and particular fauna and flora (cranes and other birds flying through clouds of peonies, cherry blossoms and chrysanthemums) were very popular motifs (see Martin and Koda). Rather than authenticity, these designs heightened the Western-based romantic stereotypes associated with a stylised form of Japanese life, conducted sedately under rule of the Japanese Imperial Court. In reality, prior to the Meiji period (1868–1912), the Emperor was largely removed from everyday concerns, residing as an isolated, holy figure in Kyoto, the traditional capital of Japan. Japan was instead ruled from Edo (modern day Tokyo) led by the Shogun and his generals, according to a strict Confucian influenced code (see Keene). In Japan, as elsewhere, the presence of feudal-style governance includes policies that determine much of everyday life, including restrictions on clothing (Rall 169). The Samurai code was no different, and included a series of protocols that restricted rank, movement, behaviour, and clothing. As Vincent has noted in the case of the ‘lace tax’ in Great Britain, these restrictions were designed to punish those who seek to penetrate the upper classes through their costume (28-30). In Japan, pre-Meiji sumptuary laws, for example, restricted the use of gold, and prohibited the use of a certain shade of red by merchant classes (V&A Kimono).Therefore, in the governance of pre-globalised societies, the importance of clothing and textile is evident; as Jones and Stallybrass comment: We need to understand the antimatedness of clothes, their ability to “pick up” subjects, to mould and shape them both physically and socially—to constitute subjects through their power as material memories […] Clothing is a worn world: a world of social relations put upon the wearer’s body. (2-3, emphasis added)The significant re-imagining of Japanese cultural and national identities are explored here through the cataclysmic impact of Western ideologies on Japanese cultural traditions. There are many ways to examine how indigenous cultures respond to European, British, or American (hereafter Western) influences, particularly in times of conflict (Wilk). Western ideology arrived in Japan after a long period of isolation (during which time Japan’s only contact was with Dutch traders) through the threat of military hostility and war. It is after this outside threat was realised that Japan’s adoption of military and industrial practices begins. The re-imagining of their national identity took many forms, and the inclusion of a Western-style military costuming as a schoolboy uniform became a highly visible indicator of Japan’s mission to protect its sovereign integrity. A brief history of Japan’s rise from a collection of isolated feudal states to a unified military power, in not only the Asian Pacific region but globally, demonstrates the speed at which they adopted the Western mode of warfare. Gunboats on Japan’s ShorelinesJapan was forcefully opened to the West in the 1850s by America under threat of First Name Perry’s ‘gunboat diplomacy’ (Hillsborough 7-8). Following this, Japan underwent a rapid period of modernisation, and an upsurge in nationalism and military expansion that was driven by a desire to catch up to the European powers present in the Pacific. Noted by Ian Ferguson in Civilization: The West and the Rest, Unsure, the Japanese decided […] to copy everything […] Japanese institutions were refashioned on Western models. The army drilled like Germans; the navy sailed like Britons. An American-style system of state elementary and middle schools was also introduced. (221, emphasis added)This was nothing short of a wide-scale reorganisation of Japan’s entire social structure and governance. Under the Emperor Meiji, who wrested power from the Shogunate and reclaimed it for the Imperial head, Japan steamed into an industrial revolution, achieving in a matter of years what had taken Europe over a century.Japan quickly became a major player-elect on the world stage. However, as an island nation, Japan lacked the essentials of both coal and iron with which to fashion not only industrial machinery but also military equipment, the machinery of war. In 1875 Japan forced Korea to open itself to foreign (read: Japanese) trade. In the same treaty, Korea was recognised as a sovereign nation, separate from Qing China (Tucker 1461). The necessity for raw materials then led to the Sino-Japanese War (1894–95), a conflict between Japan and China that marked the emergence of Japan as a major world power. The Korean Peninsula had long been China’s most important client state, but its strategic location adjacent to the Japanese archipelago, and its natural resources of coal and iron, attracted Japan’s interest. Later, the Russo-Japanese War (1904–05), allowed a victorious Japan to force Russia to abandon its expansionist policy in the Far East, becoming the first Asian power in modern times to defeat a European power. The Russo-Japanese War developed out of the rivalry between Russia and Japan for dominance in Korea and Manchuria, again in the struggle for natural resources (Tucker 1534-46).Japan’s victories, together with the county’s drive for resources, meant that Japan could now determine its role within the Asia-Pacific sphere of influence. As Japan’s military, and their adoption of Westernised combat, proved effective in maintaining national integrity, other social institutions also looked to the West (Ferguson 221). In an ironic twist—while Victorian and Continental fashion was busy adopting the exotic, oriental look (Martin and Koda)—the kimono, along with other essentials of Japanese fashions, were rapidly altered (both literally and figuratively) to suit new, warlike ideology. It should be noted that kimono literally means ‘things that you wear’ and which, prior to exposure to Western fashions, signified all worn clothing (Dalby 65-119). “Wearing Things” in Westernised JapanAs Japan modernised during the late 1800s the kimono was positioned as symbolising barbaric, pre-modern, ‘oriental’ Japan. Indeed, on 17 January 1887 the Meiji Empress issued a memorandum on the subject of women’s clothing in Japan: “She [the Empress] believed that western clothes were in fact closer to the dress of women in ancient Japan than the kimonos currently worn and urged that they be adopted as the standard clothes of the reign” (Keene 404). The resemblance between Western skirts and blouses and the simple skirt and separate top that had been worn in ancient times by a people descended from the sun goddess, Amaterasu wo mikami, was used to give authority and cultural authenticity to Japan’s modernisation projects. The Imperial Court, with its newly ennobled European style aristocrats, exchanged kimono silks for Victorian finery, and samurai armour for military pomp and splendour (Figure 1).Figure 1: The Meiji Emperor, Empress and Crown Prince resplendent in European fashions on an outing to Asukayama Park. Illustration: Toyohara Chikanobu, circa 1890.It is argued here that the function of a uniform is to prepare the body for service. Maids and butlers, nurses and courtesans, doctors, policemen, and soldiers are all distinguished by their garb. Prudence Black states: “as a technology, uniforms shape and code the body so they become a unit that belongs to a collective whole” (93). The requirement to discipline bodies through clothing, particularly through uniforms, is well documented (see Craik, Peoples, and Foucault). The need to distinguish enemies from allies on the battlefield requires adherence to a set of defined protocols, as referenced in military fashion compendiums (see Molloy). While the postcolonial adoption of Western-based clothing reflects a new form of subservience (Rall, Kuechler and Miller), in Japan, the indigenous garments were clearly designed in the interests of ideological allegiance. To understand the Japanese sartorial traditions, the kimono itself must be read as providing a strong disciplinary element. The traditional garment is designed to represent an upright and unbending column—where two meters of under bindings are used to discipline the body into shape are then topped with a further four meters of a stiffened silk obi wrapped around the waist and lower chest. To dress formally in such a garment requires helpers (see Dalby). The kimono both constructs and confines the women who wear it, and presses them into their roles as dutiful, upper-class daughters (see Craik). From the 1890s through to the 1930s, when Japan again enters a period of militarism, the myth of the kimono again changes as it is integrated into the build-up towards World War II.Decades later, when Japan re-established itself as a global economic power in the 1970s and 1980s, the kimono was re-authenticated as Japan’s ‘traditional’ garment. This time it was not the myth of a people descended from solar deities that was on display, but that of samurai strength and propriety for men, alongside an exaggerated femininity for women, invoking a powerful vision of Japanese sartorial tradition. This reworking of the kimono was only possible as the garment was already contained within the framework of Confucian family duty. However, in the lead up to World War II, Japanese military advancement demanded of its people soldiers that could win European-style wars. The quickest solution was to copy the military acumen and strategies of global warfare, and the costumes of the soldiery and seamen of Europe, including Great Britain (Ferguson). It was also acknowledged that soldiers were ‘made not born’ so the Japanese educational system was re-vamped to emulate those of its military rivals (McVeigh). It was in the uptake of schoolboy uniforms that this re-imagining of Japanese imperial strength took place.The Japanese Schoolboy UniformCentral to their rapid modernisation, Japan adopted a constitutional system of education that borrowed from American and French models (Tipton 68-69). The government viewed education as a “primary means of developing a sense of nation,” and at its core, was the imperial authorities’ obsession with defining “Japan and Japaneseness” (Tipton 68-69). Numerous reforms eventually saw, after an abolition of fees, nearly 100% attendance by both boys and girls, despite a lingering mind-set that educating women was “a waste of time” (Tipton 68-69). A boys’ uniform based on the French and Prussian military uniforms of the 1860s and 1870s respectively (Kinsella 217), was adopted in 1879 (McVeigh 47). This jacket, initially with Prussian cape and cap, consists of a square body, standing mandarin style collar and a buttoned front. It was through these education reforms, as visually symbolised by the adoption of military style school uniforms, that citizen making, education, and military training became interrelated aspects of Meiji modernisation (Kinsella 217). Known as the gakuran (gaku: to study; ran: meaning both orchid, and a pun on Horanda, meaning Holland, the only Western country with trading relations in pre-Meiji Japan), these jackets were a symbol of education, indicating European knowledge, power and influence and came to reflect all things European in Meiji Japan. By adopting these jackets two objectives were realised:through the magical power of imitation, Japan would, by adopting the clothing of the West, naturally rise in military power; and boys were uniformed to become not only educated as quasi-Europeans, but as fighting soldiers and sons (suns) of the nation.The gakuran jacket was first popularised by state-run schools, however, in the century and a half that the garment has been in use it has come to symbolise young Japanese masculinity as showcased in campus films, anime, manga, computer games, and as fashion is the preeminent garment for boybands and Japanese hipsters.While the gakuran is central to the rise of global militarism in Japan (McVeigh 51-53), the jacket would go on to form the basis of the Sun Yat Sen and Mao Suits as symbols of revolutionary China (see McVeigh). Supposedly, Sun Yat Sen saw the schoolboy jacket in Japan as a utilitarian garment and adopted it with a turn down collar (Cumming et al.). For Sun Yat Sen, the gakuran was the perfect mix of civilian (school boy) and military (the garment’s Prussian heritage) allowing him to walk a middle path between the demands of both. Furthermore, the garment allowed Sun to navigate between Western style suits and old-fashioned Qing dynasty styles (Gerth 116); one was associated with the imperialism of the National Products Movement, while the other represented the corruption of the old dynasty. In this way, the gakuran was further politicised from a national (Japanese) symbol to a global one. While military uniforms have always been political garments, in the late 1800s and early 1900s, as the world was rocked by revolutions and war, civilian clothing also became a means of expressing political ideals (McVeigh 48-49). Note that Mahatma Ghandi’s clothing choices also evolved from wholly Western styles to traditional and emphasised domestic products (Gerth 116).Mao adopted this style circa 1927, further defining the style when he came to power by adding elements from the trousers, tunics, and black cotton shoes worn by peasants. The suit was further codified during the 1960s, reaching its height in the Cultural Revolution. While the gakuran has always been a scholarly black (see Figure 2), subtle differences in the colour palette differentiated the Chinese population—peasants and workers donned indigo blue Mao jackets, while the People’s Liberation Army Soldiers donned khaki green. This limited colour scheme somewhat paradoxically ensured that subtle hierarchical differences were maintained even whilst advocating egalitarian ideals (Davis 522). Both the Sun Yat Sen suit and the Mao jacket represented the rejection of bourgeois (Western) norms that objectified the female form in favour of a uniform society. Neo-Maoism and Mao fever of the early 1990s saw the Mao suit emerge again as a desirable piece of iconic/ironic youth fashion. Figure 2: An example of Gakuran uniform next to the girl’s equivalent on display at Ichikawa Gakuen School (Japan). Photo: Emerald King, 2015.There is a clear and vital link between the influence of the Prussian style Japanese schoolboy uniform on the later creation of the Mao jacket—that of the uniform as an integral piece of worn propaganda (Atkins).For Japan, the rapid deployment of new military and industrial technologies, as well as a sartorial need to present her leaders as modern (read: Western) demanded the adoption of European-style uniforms. The Imperial family had always been removed from Samurai battlefields, so the adoption of Western military costume allowed Japan’s rulers to present a uniform face to other global powers. When Japan found itself in conflict in the Asia Pacific Region, without an organised military, the first requirement was to completely reorganise their system of warfare from a feudal base and to train up national servicemen. Within an American-style compulsory education system, the European-based curriculum included training in mathematics, engineering and military history, as young Britons had for generations begun their education in Greek and Latin, with the study of Ancient Greek and Roman wars (Bantock). It is only in the classroom that ideological change on a mass scale can take place (Reference Please), a lesson not missed by later leaders such as Mao Zedong.ConclusionIn the 1880s, the Japanese leaders established their position in global politics by adopting clothing and practices from the West (Europeans, Britons, and Americans) in order to quickly re-shape their country’s educational system and military establishment. The prevailing military costume from foreign cultures not only disciplined their adopted European bodies, they enforced a new regime through dress (Rall 157-174). For boys, the gakuran symbolised the unity of education and militarism as central to Japanese masculinity. Wearing a uniform, as many authors suggest, furthers compliance (Craik, Nagasawa Kaiser and Hutton, and McVeigh). As conscription became a part of Japanese reality in World War II, the schoolboys just swapped their military-inspired school uniforms for genuine military garments.Re-imagining a Japanese schoolboy uniform from a European military costume might suit ideological purposes (Atkins), but there is more. The gakuran, as a uniform based on a close, but not fitted jacket, was the product of a process of advanced industrialisation in the garment-making industry also taking place in the 1800s:Between 1810 and 1830, technical calibrations invented by tailors working at the very highest level of the craft [in Britain] eventually made it possible for hundreds of suits to be cut up and made in advance [...] and the ready-to-wear idea was put into practice for men’s clothes […] originally for uniforms for the War of 1812. (Hollander 31) In this way, industrialisation became a means to mass production, which furthered militarisation, “the uniform is thus the clothing of the modern disciplinary society” (Black 102). There is a perfect resonance between Japan’s appetite for a modern military and their rise to an industrialised society, and their conquests in Asia Pacific supplied the necessary material resources that made such a rapid deployment possible. The Japanese schoolboy uniform was an integral part of the process of both industrialisation and militarisation, which instilled in the wearer a social role required by modern Japanese society in its rise for global power. Garments are never just clothing, but offer a “world of social relations put upon the wearer’s body” (Jones and Stallybrass 3-4).Today, both the Japanese kimono and the Japanese schoolboy uniform continue to interact with, and interrogate, global fashions as contemporary designers continue to call on the tropes of ‘military chic’ (Tonchi) and Japanese-inspired clothing (Kawamura). References Atkins, Jaqueline. Wearing Propaganda: Textiles on the Home Front in Japan, Britain, and the United States. Princeton: Yale UP, 2005.Bantock, Geoffrey Herman. Culture, Industrialisation and Education. London: Routledge & K. Paul, 1968.Black, Prudence. “The Discipline of Appearance: Military Style and Australian Flight Hostess Uniforms 1930–1964.” Fashion & War in Popular Culture. Ed. Denise N. Rall. Bristol: Intellect/U Chicago P, 2014. 91-106.Craik, Jenifer. Uniforms Exposed: From Conformity to Transgression. 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