Thematische Bibliographien / MS TMT

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Auswahl der wissenschaftlichen Literatur zum Thema „MS TMT“

Autor: Grafiati

Veröffentlicht am 28. Juni 2021

Zuletzt aktualisiert am 8. Februar 2022

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Zeitschriftenartikel zum Thema "MS TMT"

Shen, Jianqiao, Vishwajeeth R. Pagala, Alex M. Breuer, Junmin Peng, Bin Ma und Xusheng Wang. „Spectral Library Search Improves Assignment of TMT Labeled MS/MS Spectra“. Journal of Proteome Research 17, Nr. 9 (10.08.2018): 3325–31. http://dx.doi.org/10.1021/acs.jproteome.8b00594.

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Khan, Omar Farooq, Ellen R. Cusano, Soundouss Raissouni, Mica Pabia, Johanna Haeseker, Nicholas Bosma, Jenny J. Ko, Aalok Kumar, Michael M. Vickers und Patricia A. Tang. „Immediate-term chemotherapy-related cognitive impairment (CRCI) following administration of intravenous (IV) chemotherapy.“ Journal of Clinical Oncology 35, Nr. 5_suppl (10.02.2017): 146. http://dx.doi.org/10.1200/jco.2017.35.5_suppl.146.

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146 Background: The acute impact of chemotherapy on cognition is unknown. This study utilized performance on the psychomotor vigilance task (PVT) and trail-making test B (TMT) to assess CRCI immediately following chemotherapy administration. Methods: Patients aged 18-80 years receiving first-line IV chemotherapy for any stage of breast or colorectal cancer were eligible. Patients with brain metastases, neurologic disorders or allergic reactions to chemotherapy were excluded. Patient symptoms, peripheral neuropathy and Stanford Sleepiness Scale were assessed. A five-minute PVT and TMT were completed on a tablet computer pre-chemotherapy and immediately post-chemotherapy. Paired Wilcoxon Rank Sum tests were used to assess changes in median PVT reaction time, TMT completion time, TMT errors and PVT lapses. A priori, increases of 20 ms or over in median PVT reaction times (approximating reaction time changes with blood alcohol concentrations of 0.04 to 0.05 g%) were considered clinically relevant. Results: 144 patients (74 breast, 70 colorectal, median age 55.5 years) were tested. Post-chemotherapy, median PVT reaction time slowed by an average of 12.4 ms (p = 0.01). Post-chemotherapy median PVT times slowed by over 20 ms in 59 patients (40.9%). TMT completion post-chemotherapy was faster by an average of 6.1 seconds (p < 0.001). No differences were seen in TMT errors (p = 0.417) or PVT lapses (p = 0.845). Change in median PVT reaction time was not associated with age, gender, number of prior chemotherapy cycles, use of paclitaxel (which contains alcohol), peripheral neuropathy grade, or self-reported anxiety, fatigue or depression. Conclusions: Median PVT reaction time was significantly slower immediately after chemotherapy compared to a pre-chemotherapy baseline, and impairment correlating to effects of alcohol was seen in 41% of patients. This effect appears independent of age, self-reported symptoms or prior chemotherapy cycles. Further studies assessing the functional implications of immediate-term CRCI are warranted.

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Baetge, Sharon Jean, Michael Dietrich, Melanie Filser, Alina Renner, Nathalie Stute, Marcia Gasis, Margit Weise et al. „Association of Retinal Layer Thickness With Cognition in Patients With Multiple Sclerosis“. Neurology - Neuroimmunology Neuroinflammation 8, Nr. 4 (27.05.2021): e1018. http://dx.doi.org/10.1212/nxi.0000000000001018.

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ObjectiveRetinal layer thickness (RLT) measured by optical coherence tomography (OCT) is considered a noninvasive, cost-efficient marker of neurodegeneration in multiple sclerosis (MS). We aimed to investigate associations of RLT with cognitive performance and its potential as indicator of cognitive status in patients with MS by performing generalized estimating equation (GEE) analyses.MethodsIn this cross-sectional study, patients with at least mild signs of cognitive impairment were examined by OCT as well as by the Brief International Cognitive Assessment for MS and tests assessing attention and executive functions (Trail Making Test [TMT] A and B). Associations of these factors were investigated using GEE models controlling for demographic and disease-related factors and correcting for multiple testing.ResultsA total of 64 patients entered the study. In the final sample (n = 50 [n = 14 excluded due to missing data or drop-outs]; n = 44 relapsing-remitting MS and n = 6 secondary progressive MS, mean Expanded Disability Status Scale score = 2.59 [SD = 1.17], disease duration [median] = 7.34 [interquartile range = 12.1]), 36.0% were cognitively impaired. RLT of the macular retinal nerve fiber layer was associated with performance in TMT-B (β = −0.259). Analyses focusing on the upper and lower tertile of RLT additionally revealed associations between macular ganglion cell-inner plexiform layer and TMT-B and verbal short-term memory and learning, respectively.ConclusionIn patients with MS, at less advanced disease stages, RLT was especially associated with cognitive flexibility promoting OCT as a potential marker advocating further extensive neuropsychological examination.

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Khan, Omar Farooq, Ellen R. Cusano, Soundouss Raissouni, Mica Pabia, Johanna Haeseker, Nicholas Adam Bosma, Jenny J. Ko et al. „Immediate-term chemotherapy-related cognitive impairment (CRCI) following administration of intravenous (IV) chemotherapy.“ Journal of Clinical Oncology 35, Nr. 15_suppl (20.05.2017): 10084. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.10084.

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10084 Background: The acute impact of chemotherapy on cognition is unknown. This study utilized performance on the psychomotor vigilance task (PVT) and trail-making test B (TMT) to assess CRCI immediately following chemotherapy administration. Methods: Patients aged 18-80 years receiving first-line IV chemotherapy for any stage of breast or colorectal cancer were eligible. Patients with brain metastases, neurologic disorders or allergic reactions to chemotherapy were excluded. Patient symptoms, peripheral neuropathy and Stanford Sleepiness Scale were assessed. A five-minute PVT and TMT were completed on a tablet computer pre-chemotherapy and immediately post-chemotherapy. Paired Wilcoxon Rank Sum tests were used to assess change in median PVT reaction time, TMT completion time, TMT errors and PVT lapses. A priori, an increase in median PVT reaction times by over 20 ms (approximating reaction time changes with blood alcohol concentrations of 0.04 to 0.05 g%) was considered a clinically relevant change. Results: 144 patients (74 breast, 70 colorectal, median age 55.5 years) were tested. Post-chemotherapy, median PVT reaction time slowed by an average of 12.4 ms ( p = 0.01). Post-chemotherapy median PVT times slowed by over 20 ms in 59 patients (40.9%). TMT completion post-chemotherapy was faster by an average of 6.1 seconds ( p < 0.001). No differences were seen in TMT errors ( p = 0.417) or PVT lapses ( p = 0.845). Change in median PVT reaction time was not associated with age, gender, number of prior chemotherapy cycles, peripheral neuropathy grade, self-reported symptoms (anxiety, fatigue or depression). Change in median PVT reaction time was also not significantly associated with use of any specific chemotherapeutic drug or class, including paclitaxel (which includes ethanol as an excipient). Conclusions: Median PVT reaction time was significantly slower immediately after chemotherapy compared to a pre-chemotherapy baseline, and impairment correlating to effects of alcohol was seen in 41% of patients. This effect appears independent of age, self-reported symptoms or prior chemotherapy cycles. Further studies assessing functional impact of immediate-term CRCI are warranted.

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Sinclair, John, und John F. Timms. „Quantitative profiling of serum samples using TMT protein labelling, fractionation and LC–MS/MS“. Methods 54, Nr. 4 (August 2011): 361–69. http://dx.doi.org/10.1016/j.ymeth.2011.03.004.

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Huang, Ting, Meena Choi, Manuel Tzouros, Sabrina Golling, Nikhil Janak Pandya, Balazs Banfai, Tom Dunkley und Olga Vitek. „MSstatsTMT: Statistical Detection of Differentially Abundant Proteins in Experiments with Isobaric Labeling and Multiple Mixtures“. Molecular & Cellular Proteomics 19, Nr. 10 (17.07.2020): 1706–23. http://dx.doi.org/10.1074/mcp.ra120.002105.

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Tandem mass tag (TMT) is a multiplexing technology widely-used in proteomic research. It enables relative quantification of proteins from multiple biological samples in a single MS run with high efficiency and high throughput. However, experiments often require more biological replicates or conditions than can be accommodated by a single run, and involve multiple TMT mixtures and multiple runs. Such larger-scale experiments combine sources of biological and technical variation in patterns that are complex, unique to TMT-based workflows, and challenging for the downstream statistical analysis. These patterns cannot be adequately characterized by statistical methods designed for other technologies, such as label-free proteomics or transcriptomics. This manuscript proposes a general statistical approach for relative protein quantification in MS- based experiments with TMT labeling. It is applicable to experiments with multiple conditions, multiple biological replicate runs and multiple technical replicate runs, and unbalanced designs. It is based on a flexible family of linear mixed-effects models that handle complex patterns of technical artifacts and missing values. The approach is implemented in MSstatsTMT, a freely available open-source R/Bioconductor package compatible with data processing tools such as Proteome Discoverer, MaxQuant, OpenMS, and SpectroMine. Evaluation on a controlled mixture, simulated datasets, and three biological investigations with diverse designs demonstrated that MSstatsTMT balanced the sensitivity and the specificity of detecting differentially abundant proteins, in large-scale experiments with multiple biological mixtures.

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Niesta, Daniela, Immo Fritsche und Eva Jonas. „Mortality Salience and Its Effects on Peace Processes“. Social Psychology 39, Nr. 1 (Januar 2008): 48–58. http://dx.doi.org/10.1027/1864-9335.39.1.48.

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Abstract. The present paper offers a review of the relationship between existential individual threats and peace-hampering as well as peace-facilitating factors. An overwhelming bulk of literature on terror management theory (TMT) demonstrates negative effects of mortality salience such as derogation of outgroup members, prejudice, stereotyping, aggression, and racism. These negative reactions may be detrimental in peace-processes and critical in explaining intergroup conflicts, severe hostilities, and war. Complementary empirical insights derived from TMT, however, demonstrate positive effects of mortality salience (MS) that lead to prosocial reactions. The findings that are reviewed throughout this paper aim at reconciling the seemingly contradictory findings of antisocial and prosocial reactions to reminders of death. In concluding, a variety of conceivable interventions are discussed that may override genuinely detrimental consequences of MS and help to foster peace processes.

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Gich, Jordi, Jordi Freixanet, Rafael García, Joan Carles Vilanova, David Genís, Yolanda Silva, Xavier Montalban und Lluís Ramió-Torrentà. „A randomized, controlled, single-blind, 6-month pilot study to evaluate the efficacy of MS-Line!: a cognitive rehabilitation programme for patients with multiple sclerosis“. Multiple Sclerosis Journal 21, Nr. 10 (25.02.2015): 1332–43. http://dx.doi.org/10.1177/1352458515572405.

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Background: MS-Line! was created to provide an effective treatment for cognitive impairment in multiple sclerosis (MS) patients. Objective: To assess the efficacy of MS-Line!. Methods: A randomized, controlled, single-blind, 6-month pilot study. Patients were randomly assigned to an experimental group (cognitive rehabilitation with the programme) or to a control group (no cognitive rehabilitation). Randomization was stratified by cognitive impairment level. Cognitive assessment included: selective reminding test, 10/36 spatial recall test (10/36 SPART), symbol digit modalities test, paced auditory serial addition test, word list generation (WLG), FAS test, subtests of WAIS-III, Boston naming test (BNT), and trail making test (TMT). Results: Forty-three patients (22 in the experimental group, 21 in the control group) were analyzed. Covariance analysis showed significant differences in 10/36 SPART ( P=0.0002), 10/36 SPART delayed recall ( P=0.0021), WLG ( P=0.0123), LNS ( P=0.0413), BNT ( P=0.0007) and TMT-A ( P=0.010) scores between groups. Conclusions: The study showed a significant improvement related to learning and visual memory, executive functions, attention and information processing speed, and naming ability in those patients who received cognitive rehabilitation. The results suggest that MS-Line! is effective in improving cognitive impairment in MS patients.

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Nazeer, Khurram, Michael G. Janech, Jim J. C. Lin, Kevin J. Ryan, John M. Arthur und Milos N. Budisavljevic. „Changes in protein profiles during course of experimental glomerulonephritis“. American Journal of Physiology-Renal Physiology 296, Nr. 1 (Januar 2009): F186—F193. http://dx.doi.org/10.1152/ajprenal.90222.2008.

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Better characterization of the molecular mechanisms underlying glomerular cell proliferation may improve our understanding of the pathogenesis of glomerulonephritis and yield disease-specific markers. We used two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) to generate expression profiles of glomerular proteins in the course of anti-Thy-1 nephritis. Glomeruli were isolated from Wistar rats by sieving, and proteins were separated by 2DE. In preliminary studies using normal rats, we identified known glomerular proteins from microfilaments [tropomyosin (Tm)] and intermediate filaments (vimentin and lamin A), proteins involved in assembly (α-actinin-4, F-actin capping protein) and membrane cytoskeletal linking (ezrin), as well as several enzymes (protein disulfide isomerase, ATP synthase, and aldehyde dehydrogenase). Comparison of glomerular protein abundance between normal rats and rats in the early phase of anti-Thy-1 nephritis yielded 28 differentially expressed protein spots. MS analysis identified 16 differentially expressed proteins including Tm. Altered Tm abundance in the course of anti-Thy-1 nephritis was confirmed, and specific isoforms were characterized by Western blotting. We demonstrated a complex change in Tm isoform abundance in the course of anti-Thy-1 nephritis. The early mesangiolytic phase of the disease was characterized by decreased abundance of low-molecular-weight isoforms Tm5a/5b and increased abundance of high-molecular-weight isoforms Tm6, Tm1, Tm2, and Tm3. The late proliferative phase of the disease was associated with increased abundance of isoforms Tm5a/5b, Tm6, and Tm1 and decreased abundance of Tm3. Isoforms Tm4 and Tm5 remained unchanged in the course of this model of experimental glomerulonephritis. Characterization of Tm isoform abundance in the course of clinical glomerulonephritis may identify disease-specific markers.

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Fenstermacher, Erika A., Jessica Birg, Vincent Barbieri und Nathaniel Herr. „THE IMPACT OF MORTALITY SALIENCE ON COLLEGE STUDENTS’ INTENT TO HELP OLDER ADULTS“. Innovation in Aging 3, Supplement_1 (November 2019): S80. http://dx.doi.org/10.1093/geroni/igz038.311.

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Abstract Terror Management Theory (TMT) states that the awareness of one’s own death causes humans to experience intense anxiety, which must be continuously managed. Much of the research on TMT has focused on negative outcomes, rather than prosocial behavior, begging the question: “Can priming individuals with the thought of their own death trigger them to behave in ways that benefit others?”. Jonas et al. (2002), found that when mortality salience was primed prosocial behavior increased. In line with TMT, they hypothesized that people may behave in a more prosocial manner as it fits in with their personal values. The present study recruited 108 students who were randomly assigned to a mortality salience (MS) or control condition. Participants also completed baseline self-reports, which included measures of ageism, social desirability, personality, and empathy. After the study seemed to end, participants were given a disguised measure of helping behavior, which they believed to be an interest survey for a student volunteer group. Preliminary analyses indicate that those in the MS condition were more willing to be contacted to volunteer with kids than being contacted to volunteer with older adults. We also found that those in the MS condition were more likely to be contacted to volunteer with kids than those in the control condition. Our findings are consistent with previous work showing that individuals favor their ingroup when primed with their death. This reflects the importance of focused efforts on encouraging young people to identify with older adults and on promoting prosocial behavior.

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Dissertationen zum Thema "MS TMT"

Golovkova, Nataliya. „Návrh protiopatření k útokům na konektivitu vozů“. Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2021. http://www.nusl.cz/ntk/nusl-442359.

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The diploma thesis is focused on the issue of connective cars and the types of threats that can occur and how to protect against them. The general part described the general model of the car. In the next part of the work, templates were created in the Microsoft Threat Modeling Tool with threats and countermeasures to them.

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Al, Hage Yassmin. „Qualitatitive and quantitative analysis of fish spoilage processes for development of new food monitoring systems“. Master's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/9382/.

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This study investigates the growth and metabolite production of microorganisms causing spoilage of Atlantic cod (Gadus morhua) fillets packaged under air and modified atmosphere (60 % CO2, 40 % O2). Samples were provided by two different retailers (A and B). Storage of packaged fillets occurred at 4 °C and 8 °C. Microbiological quality and metabolite production of cod fillets stored in MAP 4 °C, MAP 8 °C and air were monitored during 13 days, 7 days and 3 days of storage, respectively. Volatile compounds concentration in the headspace were quantified by Selective ion flow tube mass spectrometry and a correlation with microbiological spoilage was studied. The onset of volatile compounds detection was observed to be mostly around 7 log cfu/g of total psychrotrophic count. Trimethylamine and dimethyl sulfide were found to be the dominant volatiles in all of the tested storage conditions, nevertheless there was no close correlation between concentrations of each main VOC and percentages of rejection based on sensory evaluation. According to results it was concluded that they cannot be considered as only indicators of the quality of cod fillets stored in modified atmosphere and air.

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Valla, Martin. „Systém pro zpracovaní skóre z metod identifikace proteinů v tandemové hmotnostní spektrometrii“. Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217552.

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The goal of my diploma thesis was finding a suitable method for unifying score values from various protein identification search tools in MS/MS mass spectrometry into one single score value. Data coming from the output of mass spectrometer are processed in two independent search tools Mascot and X!Tandem. These were selected especially for their wide usage in proteomic labs. Both results are evaluated through newly designed function and unified by single valued score clearly identifying found proteins. Newly designed scoring value is called Matascore and function producing this score was implemented in MATLAB. Function and its results were successfully tested by real data available in public databases on the Internet.

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Jeske, Helene [Verfasser], und Rainer [Akademischer Betreuer] Marutzky. „Methodenetablierung und Untersuchungen der Pyrolyseprozesse intumeszierender Brandschutzbeschichtungen mittels gekoppelter thermogravimetrischer und -mechanischer Analyse (TGA-FTIR-MS und TMA) sowie DSC-Mikroskopie / Helene Jeske ; Betreuer: Rainer Marutzky“. Braunschweig : Technische Universität Braunschweig, 2012. http://d-nb.info/1175823155/34.

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Hösl, Simone. „Laserablation mit induktiv gekoppelter Plasma-Massenspektrometrie für die medizinische Diagnostik“. Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät, 2017. http://dx.doi.org/10.18452/17716.

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In dieser Arbeit wurde eine neue Markierungsstrategie von Antikörpern mit dem Markierungsreagenz MeCAT (Metal Coded Tag) unter physiologischen Reaktionsbedingungen, sowie deren Anwendung in einem 8-fach Multiplex-Immunoassay von in Formalin-fixierten und in Paraffin-eingebetteten Gewebeschnitten entwickelt. Für eine aussagekräftige LA-ICP-MS Detektion von MeCAT-modifizierten Antikörpern, wurde eine Standardisierung für biologische Proben auf NC-Membranen, basierend auf einer homogenen Aufbringung eines internen Standards und Kalibrierstandards durch einen kommerziell verfügbaren Tintenstrahldrucker entwickelt und mit der ICP-MS Analyse von Lösungen evaluiert. Die LA-ICP-MS wurde in zwei 8-fach Multiplex-Immunoassays von Tissue Micro Arrays vom Prostatakarzinom und in Maushirngewebeschnitten zur Einschätzung von neurogenerative Erkrankungen erfolgreich eingesetzt werden. Es konnte hierbei gezeigt werden, dass das Nachweisvermögen, der hier entwickelten Methode bereits ausreicht, um die gängigen klinischen Biomarker mit guter Ortsauflösung nachzuweisen.
In this work a new tagging strategy of antibodies with the tagging reagent MeCAT (Metal Coded Tag) was developed under physiological reaction conditions. Their application was proved in an 8-fold multiplex immunoassay of formalin-fixed and paraffin-embedded tissue sections. For a significant LA-ICP-MS detection of MeCAT tagged antibodies standardization for biological samples owere developed. The standardization based on a homogeneous deposition onto the NC membrane via conventional CD-ink-jet printer was validated in addition with the ICP-MS analysis of solutions. The internal standardization of LA-ICP-MS was successfully applied in two 8-fold multiplex immunoassays for Tissue Micro Arrays (TMA) of prostate cancer and for detection of biomarkers for neurodegenerative diseases in mouse brain tissue sections. In both examples it could be shown that the detection capability of the new tagging strategy in combination with the printing standardization allows the detection of the clinical biomarker with good spatial resolutions.

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Zacarés, Sanmartín Laura. „Nuevas aportaciones al metabolismo secundario del tomate. Identificación y estudio de moléculas implicadas en la respuesta a la infección con pseudomonas syrinagae pv. tomato“. Doctoral thesis, Universitat Politècnica de València, 2008. http://hdl.handle.net/10251/3021.

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Los fenilpropanoides constituyen un grupo de metabolitos secundarios producidos y utilizados por las plantas como parte de la respuesta defensiva tanto constitutiva como inducible. Un gran número de ellos están implicados en la resistencia frente a la enfermedad a diferentes niveles: señalización (ácido salicílico), agentes antimicrobianos (fitoalexinas), y endurecimiento de la pared celular (lignina). Las amidas derivadas del ácido hidroxicinámico (HCAAs) son un conjunto de metabolitos, pertenecientes al grupo de los fenilpropanoides, que desempeñan un importante papel en la defensa de las plantas frente a patógenos y predadores. Las HCAAs se forman a partir de la condensación de tioésteres de hidroxicinamoil-CoA con feniletilaminas, tales como la tiramina. El último paso en la biosíntesis de las HCAAs está catalizado por el enzima tiramina hidroxicinamoil transferasa (THT). En la presente tesis se muestra la identificación y el estudio de cuatro HCAAs, p-cumaroildopamina, feruloildopamina, p-cumaroiltiramina y feruloiltiramina, asociadas a la infección de tomate con la bacteria Pseudomonas syringae pv. tomato. Su identificación y caracterización estructural se han llevado a cabo mediante técnicas de cromatografía líquida de alta resolución y espectrometría de masas (HPLC-MS). Se ha analizado la posible implicación del ácido salicílico y del etileno en la inducción patogénica de dichas moléculas y del enzima responsable de su biosíntesis (THT). Además, se ha estudiado la actividad antioxidante y antibacteriana in vitro de las cuatro HCAAs identificadas. Por último, se han obtenido líneas transgénicas de Arabidopsis thaliana y de tomate que sobreexpresan el gen de la THT, y se han analizado los perfiles cromatográficos de dichas líneas.
Zacarés Sanmartín, L. (2008). Nuevas aportaciones al metabolismo secundario del tomate. Identificación y estudio de moléculas implicadas en la respuesta a la infección con pseudomonas syrinagae pv. tomato [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/3021
Palancia

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Ruchti, Jacqueline. „Analysis of TNT, DNA Methylation, and Hair Pigmentation via Gas Chromatography-Mass Spectrometry and Spectroscopic Techniques“. Thesis, 2019. http://hdl.handle.net/1805/20023.

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Cheng, Chin-Fu, und 鄭清福. „The studies on the efficacy, toxicity, and residue of tricaine methanesulfonate (TMS;MS-222) in the tilapia, Oreochromis hybrids“. Thesis, 2014. http://ndltd.ncl.edu.tw/handle/84491856735777987669.

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博士
國立中興大學
獸醫學系暨研究所
102
This study is to research the toxicity and anesthetic effectiveness of TMS (tricaine methanesulfonate, TMS) on tilapia (Oreochromis hybrids). The method applied was high- performance liquid chromatography (HPLC) plus ultraviolet detector to examine and the residues of TMS in the tissue and serum of tilapia after eight hours of immersion in TMS. Then after, by analyzing the behavior of tilapia during the final anesthesia and resuscitation, we can divide it in to 6 and 4 stages respectively. The results show that under different temperatures and concentrations of TMS, the effectiveness of anesthesia and time of resuscitation varies. The result reveals that the time needed to reach stage A4 of anesthesia shortens as concentration of TMS and temperature rise. Also, the time needed for resuscitation is fixated around five minutes at a concentration of 340 ppm. Furthermore, when the concentration of TMS is controlled around 260~300 ppm, the time needed for tilapia to reach A4 stage and R3 stage is 3 minutes and 5 minutes respectively. These results consistent under different temperatures, thus, under proper maintenance of anesthesia, we may be able to apply it to surgery for tilapia. After observing the effectiveness of anesthesia, we performed tests based on 5 log concentration between maximum safety dose and minimum lethal dose and aimed at the toxicity of TMS for tilapia were run under a 24 hour and 96 hour time frame. The results show that the LD50 for 24 hour and 96 hour exposure were 79.23 ppm and 78.06 ppm respectively. This indicates that under the longer the exposure, the concentration for LD50 decreases. The methodology used to examine the residue of an eight hour long exposure to 30 ppm of TMS was HPLC. HPLC is the method of using McIIvaine buffer (0.1 M citric acid/ 0.2 M disodium hydrogen phosphate, 1：1) for extraction, then filtered through C18 cartridges, and set with ultraviolet detector at a wavelength of 220 nm. The results indicate that such a method is both accurate and precise, plus it also has a minimum detection limit of 0.01 ppm. TMS residues in serum, liver, and muscle of tilapia are under the minimum detection limit after 48, 192, and 24 hours respectively. This study, in addition to the establishment of the necessary reference tilapia clinical doses of different applications, the detection method can be effectively applied to the tilapia fish tissue residues or relevant aquatic products for the detection of TMS.

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Hsu, Ren-Yu, und 許仁裕. „The Investigation of GC-ESI/MS of TMS Derivative and the Development of Low EOF Hydrodynamic Flow Assisted Double Junction Interface“. Thesis, 2016. http://ndltd.ncl.edu.tw/handle/66289447296777811107.

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博士
國立臺灣大學
化學研究所
104
Gas chromatography (GC) and capillary electrophoresis (CE) are the common analytical techniques, both of them provide high separation efficiency and low sample consumption. The coupling of GC (or CE) with mass spectrometry (MS) provides a technique with high separation efficiency and high selectivity. Among the ionization techniques, electron ionization (EI) is the most commonly used ionization technique in GC/MS. Unlike EI, little fragmentation is observed under electrospray ionization (ESI). Therefore, the GC–ESI/MS combination provides a technique with high separation efficiency, high selectivity and abundant information on the molecular weight of the analyte. GC is an effective separation technique for volatile and nonpolar compounds. However, for polar analytes, derivatization is often required, because it can enhance the separation efficiency of polar compounds. Among the derivatization methods, trimethylsilylation is one of the most widely adopted approaches. A drawback of trimethylsilyl (TMS) derivatives is that they are sensitive to moisture. In aqueous environments, TMS derivatives may hydrolyze back to their native form. After GC separation, the TMS derivatives eluted from the GC column interact with the aqueous charged droplets during ESI analysis. The TMS derivatives may hydrolyze back to their native form and thus become more suitable for ESI analysis. In this work, several types of compounds (organic acids, phenols and beta-agonists) were trimethylsilylated before GC–ESI/MS analysis. The hydrolysis efficiency and application of GC-ESI/MS in TMS-derivatives were studied. Analyzing TMS-organic acids by GC–ESI/MS indicated that hydrolysis was incomplete. Both the TMS derivative ([M+TMS+H]+) and the hydrolysis product ([M-H]−) were detected. According to the results of polyprotic acids, the hydrolysis efficiency was lower with more TMS groups. Although the hydrolysis is incomplete, the detection of a TMS derivative and its hydrolysis product at the same retention time may facilitate compound confirmation. The analysis of TMS-phenols indicated that the hydrolysis efficiency was affected by the functional group attached to phenol. For example, partial hydrolysis was observed for methylphenols and nitrophenols. Both [M+TMS+H]+ and [M-H]− were detected. However, only the hydrolysis product, [M-H]−, was detected for CPs. This result suggested that TMS-CPs eluted from the GC were hydrolyzed back to their native form during the ESI process. To evaluate the limit of detection of GC-ESI/MS analysis of TMS-CPs, collision-induced dissociation (CID) was used. The LOD of TMS-CPs was estimated to be in the range of 0.25–5 ng/mL except for TMS-CPs with poor fragmentation efficiency. In GC–ESI/MS analysis of beta-agonists, only the protonated molecules of TMS-beta agonists were detected. The results suggested that the hydrolysis efficiency of TMS-beta agonists was considerably lower than that of TMS-organic acids and TMS-CPs. No hydrolysis product was detected even after the flow rate of the spray solvent was increased. The ESI mass spectra were dominated by protonated molecule signals with little fragmentation. Under CID, the LODs were estimated to be in the range of 0.5–10 ng/mL, although they did not hydrolyze back to their polar native form during ESI. Besides GC, CE is another separation technique with high separation efficiency. CE often involves adding nonvolatile additives or salts to improve the separation. However, nonvolatile additives are not suitable for ESI-MS, primarily because of their ion suppression effect. In this work, a hydrodynamic flow assisted double junction interface was used to prevent nonvolatile additives from entering the ESI source, to alleviate ion suppression caused by nonvolatile additives in CE-ESI/MS analysis. The hydrodynamic flow assisted double junction interface was fabricated with a liquid junction reservoir and conducting liquid reservoir and a short transfer column (1 cm). The hydrodynamic flow was introduced by a syringe into between the separation column and transfer column. By adjusting the hydrodynamic flow and electric field, the analytes, but not additive, were pushed to the ESI sprayer. To improve the versatility of the hydrodynamic flow assisted double junction interface, a conventional uncoated fused silica transfer column was replaced with a polyvinyl alcohol (PVA) column. Because of PVA-coated column has an extremely low electroosmotic flow (EOF), the velocity of an ion was determined by its electrophoretic mobility and the hydrodynamic flow applied to the interface. EOF no longer plays a role in the velocity of an ion. The migration behavior of the analyte and additive in transfer columns could be controlled by adjusting the hydrodynamic flow applied to the interface to alleviate the problem of ion suppression.

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Bücher zum Thema "MS TMT"

A parent's guide to video games: A practical guide to selecting and managing home video games. Post Falls, Idaho: DMS, 1991.

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NIMERSHEIM. Tmg Ms/dos 5. Longman Higher Education, 1991.

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GC-MS of Biologically and Environmentally Significant Organic Compounds: TMS Derivatives. Wiley & Sons, Limited, John, 2020.

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Materials Science & Technology 2004: AIST/TMS Proceedings : September 26-29, 2004, New Orleans, Louisiana (MS&T '04). Iron & Steel Society, 2004.

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TNT DOS-extender reference manual. Cambridge, MA: Phar Lap Software, 1993.

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Buchteile zum Thema "MS TMT"

Sinclair, John, und John F. Timms. „Proteomic Profiling of Ovarian Cancer Models Using TMT-LC-MS/MS“. In Methods in Molecular Biology, 271–84. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-547-7_20.

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O’Brien, Darragh P., und John F. Timms. „Employing TMT Quantification in a Shotgun-MS Platform“. In Shotgun Proteomics, 187–99. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0685-7_12.

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de Oliveira, Bruno Menezes. „Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscle“. In Shotgun Proteomics, 201–9. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0685-7_13.

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Erdjument-Bromage, Hediye, Fang-Ke Huang und Thomas A. Neubert. „Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS)“. In Methods in Molecular Biology, 135–49. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7659-1_11.

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Natsui, Shungo, Ryota Shibuya, Hiroshi Nogami, Tatsuya Kikuchi und Ryosuke O. Suzuki. „Synchronized High-Speed Microscopy and Thermoanalytical Measurement for Sub-mm/sub-ms-scale Cathodic Behavior in Molten Salt Electrolysis“. In TMS 2021 150th Annual Meeting & Exhibition Supplemental Proceedings, 338–45. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-65261-6_30.

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Stephanou, Euripides. „Determination of Acidic and Neutral Residues of Alkylphenol Polyethoxylate Surfactants Using GC/MS Analysis of their TMS Derivatives“. In Organic Micropollutants in the Aquatic Environment, 155–61. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-4660-6_18.

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Bagwari, Ashish, Jyotshana Kanti und Geetam Singh Tomar. „Multiple Antennas-Based Improved Sensing Detector“. In Advances in Wireless Technologies and Telecommunication, 143–63. IGI Global, 2019. http://dx.doi.org/10.4018/978-1-5225-5354-0.ch009.

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5G is one of the newest and most acclaimed engineering technology of wireless era. This technology is also known as CRNs, in which spectrum detection plays a key role. Through this chapter, the authors explore multiple antennas-based improved sensing detector (MA_ISD). In the said scheme by using adaptive threshold and multiple antennas patterns, its easy to mitigate sensing failure issue enriches reliability. The presented scheme uses two detectors (TD) concept. The concept of two detectors (i.e., TD scheme has been applied in which multiple antennas are used for electing the quality signals). The considered model upgrades the detection operation and acquires limited or inferior detection time. The first energy detector uses a single adaptive threshold (ED-SAT) while another energy detector employs two adaptive thresholds (ED-TAT). The threshold value is accommodative as it relays on noise variance (σ_ω^2), the behavior of noise variance transforms in accordance with noise signal. Both the detectors run collaboratively and their gain is then supply to a decision device which operates OR functions. In this research work, results shows that with cooperation of two antennas (Nr=2) in multiple antennas-based improved sensing detector (MA_ISD) technique delivers enhancement in detection performance by 24.6%, 53.4%, 37.9%, and 49.6%, in contrast to existing schemes (i.e., EDT-ASS-2015 scheme, ED and cyclo-2010, adaptive SS-2012, and conventional-ED) scheme at -12dB SNR, respectively. During the time, proposed technique also reduces the sensing time in the order of 47.0 ms, 49.0 ms, and 53.2 ms as compared to existing schemes (EDT-ASS-2015, Adaptive SS-2012, and ED and Cyclo-2010) scheme at - 20 dB SNR, respectively.

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Konferenzberichte zum Thema "MS TMT"

Ramirez-San-Juan, Julio C., Alia T. Tuqan, Kristen M. Kelly, J. Stuart Nelson und Guillermo Aguilar. „Evaluation of Sub-Zero and Residence Times After Continuous Versus Multiple Intermittent Cryogen Spray Cooling Exposure on Human Skin Phantom“. In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-59635.

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Cryogen spray cooling (CSC) is used to minimize the risk of epidermal damage during various laser dermatologic surgeries. However, as the application of single or multiple cryogen spurts becomes available on some commercial lasers devices, it is necessary to determine the optimal CSC parameters for different laser surgeries. The objective of this study was to measure the time the sprayed surface of a human skin phantom (HSP) remains below water freezing temperature 0°C, referred to as subzero time (ts), and below the cryogen boiling temperature −26°C, referred to as residence time (tr), as well as the minimum surface temperature (Tmin) and the time at which Tmin occurs (tTmin) for two HSP-initial temperatures (20 °C and 70 °C) during and after the application of single (SCS) and multiple cryogen spurts (MCS). For this propose, a HSP was used to measure ts, tr, Tmin, and tTmin for nine sequences: one SCS of ΔtT = TCT = 40 ms; four MCS sequences, all adding to a ΔtT of 40 ms but with different TCT up to 110 ms and, finally; four SCS that matched the TCT of the four MCS sequences, but lead to different ΔtT. Our results show that the differences between SCS and MCS sequences with the same TCT are negligible for all variables measured (ts, tr, Tmin, tTmin). Moreover, in this interval (40 ms ≤ TCT ≤ 110 ms), this variables show a remarkable linear dependence with the TCT.

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Benda, Martin, Jakub Vrbka und Patrik Scudla. „Multifunctional Footwalk for TMS, MS and MMS“. In 2019 International Conference on Military Technologies (ICMT). IEEE, 2019. http://dx.doi.org/10.1109/miltechs.2019.8870108.

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HONDA, M., K. ONO, S. TARUCHA, T. SATO, T. MANAGO und H. AKINAGA. „ENHANCED TMR SIGNAL IN A SPIN-VALVE TRANSISTOR“. In Toward the Controllable Quantum States - International Symposium on Mesoscopic Superconductivity and Spintronics (MS+S2002). WORLD SCIENTIFIC, 2003. http://dx.doi.org/10.1142/9789812705556_0008.

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Wang, Yunping, Xiaoming Ji und Xinbo Ruan. „The output property of photovoltaic MS and TCT configurations under partially shaded conditions“. In 2017 IEEE 26th International Symposium on Industrial Electronics (ISIE). IEEE, 2017. http://dx.doi.org/10.1109/isie.2017.8001389.

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Rui, Xiaoting, Guoping Wang, Laifeng Yun, Bin He, Fufeng Yang und Bao Rong. „Advances in Transfer Matrix Method of Multibody System“. In ASME 2009 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2009. http://dx.doi.org/10.1115/detc2009-86433.

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Multibody system dynamics has become important theoretical tool for wide engineering problems analysis in the world. Transfer matrix method of multibody system (MS-TMM) is a new approach for multibody system dynamics, which is developed in 20 years. In this paper, the transfer matrix method for linear and nonlinear multibody systems are introduced respectively. For linear multibody systems, the new concept of body dynamics equation and augmented eigenvector, the construction method of orthogonality, and the computing method of vibration characteristics and dynamic response are introduced; For nonlinear multibody systems, the discrete time transfer matrix method of multibody system (MS-DT-TMM) are presented. The apply of the transfer matrix method for multibody systems with tree, closed loop and network structures are also introduced. The transfer matrix method has good characteristics: 1 It does not require overall dynamics equations of system and simplify the solution procedure. 2 It has high computing speed, because the system matrices are always small irrespective of the size of a system. 3 It avoids the difficulties caused by developing overall dynamic equations of a system and by computing too high order matrices. 4 It provides maximum flexibility in modeling various configurations of multibody systems, by creating library of transfer matrices and assembling them easily, and by introducing any suitable numerical integration scheme. The new method is efficient for linear and nonlinear multi-rigid-flexible-body system, and it has been paid great attention, because many engineering problem of important mechanical system were solved effectively by using this method.

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Smith, D. E. „True Government Take (TGT): A Measurement of Fiscal Terms“. In SPE Hydrocarbon Economics and Evaluation Symposium. Society of Petroleum Engineers, 1987. http://dx.doi.org/10.2118/16308-ms.

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Hiekal, S. A., G. M. Hamada und M. Khalil. „Gas Saturation Detection Model Applied To Heterogeneous Reservoir Using (TDT)“. In Middle East Oil Show and Conference. Society of Petroleum Engineers, 1997. http://dx.doi.org/10.2118/37777-ms.

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Lim, Hwee San, Mohd Zubir Mat Jafri und Khiruddin Abdullah. „A New Algorithm for Retrieval of Air Quality from Landsat TM7“. In SPE Asia Pacific Health, Safety and Environment Conference and Exhibition. Society of Petroleum Engineers, 2005. http://dx.doi.org/10.2118/95410-ms.

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Hamada, Gharib Moustafa, und Ahmed Hegazy. „Hydrocarbon Potential Monitoring in Gas Sandstone Reservoir Using CHFR and TDT Techniques“. In EUROPEC/EAGE Conference and Exhibition. Society of Petroleum Engineers, 2007. http://dx.doi.org/10.2118/105003-ms.

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Hamada, Gharib Moustafa. „Hydrocarbon Potential Monitoring of By-passed Pay Zones Using TDT and CHFR Logs“. In Asia Pacific Oil and Gas Conference and Exhibition. Society of Petroleum Engineers, 2007. http://dx.doi.org/10.2118/106748-ms.

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