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Auswahl der wissenschaftlichen Literatur zum Thema „Microscopie haute résolutive“
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Zeitschriftenartikel zum Thema "Microscopie haute résolutive"
Baida, Fadi, Claudine Bainier und Daniel Courjon. „Interférometrie haute résolution par microscopie optique en champ proche“. Microscopy Microanalysis Microstructures 5, Nr. 4-6 (1994): 409–25. http://dx.doi.org/10.1051/mmm:0199400504-6040900.
Der volle Inhalt der QuelleVincent Tarazona, Par. „Système d'analyse à haute résolution pour microscopie électronique à transmission“. Biofutur 1997, Nr. 169 (Juli 1997): 6–8. http://dx.doi.org/10.1016/s0294-3506(97)84146-4.
Der volle Inhalt der QuellePénisson, J. M. „Analyse chimique d'un superalliage base nickel par microscopie électronique à haute résolution“. Le Journal de Physique IV 06, Nr. C2 (März 1996): C2–117—C2–123. http://dx.doi.org/10.1051/jp4:1996216.
Der volle Inhalt der QuelleBonnet, R., M. Loubradou und J. M. Pénisson. „Microscopie à haute résolution d'une interface semi-cohérente phase µ/matrice γ-γ'“. Le Journal de Physique IV 06, Nr. C2 (März 1996): C2–165—C2–170. http://dx.doi.org/10.1051/jp4:1996223.
Der volle Inhalt der QuelleBres, E. „Etude des interfaces de phosphates de calcium par microscopie électronique à haute résolution.“ Revue de Métallurgie 91, Nr. 9 (September 1994): 1303. http://dx.doi.org/10.1051/metal/199491091303.
Der volle Inhalt der QuelleHaddadi, K., D. Szymik, V. Hoel und G. Dambrine. „Développement de solutions hyperfréquences pour la société moderne : Bio-radar et Microscopie électromagnétique“. J3eA 18 (2019): 1013. http://dx.doi.org/10.1051/j3ea/20191013.
Der volle Inhalt der QuelleWang, Y., M. Gandais und A. I. Ekimov. „Couche mince de silice dopée en nanocristaux de CDS Microscopie électronique en transmission à haute résolution, microanalyse“. Revue de Métallurgie 90, Nr. 9 (September 1993): 1204. http://dx.doi.org/10.1051/metal/199390091204.
Der volle Inhalt der QuelleFredj, A. Ben, und G. Nihoul. „Observation en microscopie électronique à haute résolution d'oxydes à grande maille (c-Dy2O3) et interprétation des images obtenues“. physica status solidi (a) 91, Nr. 2 (16.10.1985): 351–69. http://dx.doi.org/10.1002/pssa.2210910204.
Der volle Inhalt der QuellePénisson, J. M., M. Bacia und T. Vystavel. „Étude par microscopie à haute résolution et imagerie filtrée de la ségrégation et du début de précipitation dans les joints de grains“. Le Journal de Physique IV 09, PR4 (April 1999): Pr4–123—Pr4–128. http://dx.doi.org/10.1051/jp4:1999415.
Der volle Inhalt der QuelleColaitis, D., W. Coene, S. Amelinckx, L. Brohan und R. Marchand. „Structures incommensurables modulées par des défauts dans la transition de phase du bronze de titane Na1−xTi4O8: Etude par diffraction électronique et microscopie électronique de haute résolution“. Journal of Solid State Chemistry 75, Nr. 1 (Juli 1988): 156–74. http://dx.doi.org/10.1016/0022-4596(88)90313-1.
Der volle Inhalt der QuelleDissertationen zum Thema "Microscopie haute résolutive"
Bai, Jiachuan. „Deep learning augmented single molecule localization microscopy reconstruction : enhancing robustness and moving towards live cells“. Electronic Thesis or Diss., Sorbonne université, 2023. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2023SORUS337.pdf.
Der volle Inhalt der QuelleWhile microscopy has been a central technique for cell biology since centuries, it has long been limited by diffraction to a resolution of ~200-300 nm. As a consequence, many molecular structures, such as viruses, nuclear pores, or microtubules were left unresolved. Single-molecule localization microscopy (SMLM) offers a high spatial resolution (e.g., 20 nm or better), allowing to resolve biological structures at or near the molecular scale. However, SMLM acquisition necessitates acquiring many thousands of low-resolution frames, mostly limiting its applications to fixed cells or to structures undergoing slow dynamics. To overcome this limitation, Ouyang et al. (2018) developed a deep learning-based approach called ANNA-PALM that can reconstruct a super-resolution image from much fewer low-resolution frames. However, the original ANNA-PALM method faced several limitations. First, ANNA-PALM had only been trained and tested on images from our laboratory. Second, the method exhibits artifacts when applied to images obtained using different protocols or experimental conditions than the training data. Third, ANNA-PALM had only been demonstrated on fixed cells. The objectives of my Ph.D. thesis are to address these limitations by 1) improving the robustness of ANNA-PALM reconstructions when applied to data obtained from distinct laboratories and 2) extending ANNA-PALM to reconstruct super-resolved time-lapse image sequences for dynamic biologicalstructures in live cells. 1. Improving the robustness of ANNA PALM: an obvious approach to improve robustness is to retrain the model using a larger and more varied data set. However, SMLM datasets are not usually publicly accessible. To address this, our lab developed ShareLoc, an online platform (shareloc.xyz) that allows the gathering and reuse of SMLM datasets acquired by the microscopy community. I first validated the platform's functionalities, curated SMLM data, implemented a ShareLoc ontology, and wrote relevant documentation. Next, I took advantage of ShareLoc data to retrain ANNA-PALM on larger and more diverse images and quantitatively evaluated the image reconstruction quality compared to the original model. I demonstrated that the robustness and reconstruction quality of ANNA-PALM significantly improved, notably when applied to images of microtubules taken under biological perturbation conditions never seen by the model during training. 2. Extending ANNA-PALM to reconstruct super-resolution movies of moving structures in live cells: achieving high-quality super-resolution reconstructions of structural dynamics is challenging. To avoid motion blur, each frame of the reconstructed movie is defined from localizations in only a small number of consecutive low-resolution frames. This leads to a strong under-sampling of the structures by single molecule localization events and does not enable super-resolution. Although ANNA-PALM can reconstruct high-quality super-resolved images from under-sampled localization data, training ANNA-PALM for live cells is more difficult, because a clear ground truth is lacking. The absence of ground truth also makes it difficult to assess reconstruction quality. To address these challenges, I first developed a method to generate ground truth super-resolution movies from static SMLM images obtained from long acquisition sequences. I implemented and tested this strategy using both simulated and experimental SMLM images. Second, I extended the ANNA-PALM architecture to 3D data, where the third dimension is time, in order to incorporate temporal information. I used simulations of microtubule dynamics to quantitatively evaluate the reconstruction quality of this approach in comparison with the original 2D ANNA-PALM, and as function of structure velocity and localization rates. [...]
Liu, Hui. „Microscopie tomographique diffractive et profilométrie multivue à haute résolution“. Thesis, Mulhouse, 2014. http://www.theses.fr/2014MULH9558/document.
Der volle Inhalt der QuelleWe have developed a tomographic diffractive microscope in reflection, which permits observation of sample surfaces with an improved lateral resolution, compared to a conventional holographic microscope. From the same set of data, high-precision measurements can be performed on the shape of the reflective surface by reconstructing the phase of the diffracted field. doing so allows for several advantages compared to classical holographic interferometric measurements: improvement in lateral resolution, easier phase unwrapping, reduction of the coherent noise, combined with the high-longitudinal precision provided by interferometric phase measurements. We demonstrate these capabilities by imaging various test samples
Gardeazabal, Rodriguez Pedro Felipe. „Développement d'une technique d'imagerie 3D haute résolution“. Paris 6, 2008. http://www.theses.fr/2008PA066594.
Der volle Inhalt der QuelleMeignen, Pierre-Antoine. „Capteur ultrasonore multiélément dédié à la caractérisation quantitative haute résolution“. Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT267/document.
Der volle Inhalt der QuelleThe work presented in this thesis is applied to the characterization of mechanical properties by acoustic microscopy. It describes an innovative focused sensor that enables both topography and quantitative imaging of an elastic material. The innovation consists in the separation of the different propagation modes of a material excited by a focused multielement probe. Measuring the surface mode propagation velocity of elastic and anisotropic materials thanks to their time of flight provides a possibility of quantifying the module characterizing the elasticity: the Young's modulus. The dimensions of the multielement probe are described here and rely on an acoustic field model developed to anticipate the field radiated by each element. A second model studies the temporal behaviour of the focused probe and also verifies the discrimination of the different waves that propagate. The measurement of mechanical properties by the multielement probe is applied to different samples and provides consistent results with high sensitivity. The ability to produce images of mechanical properties is thus demonstrated. First suitable for frequencies near thirty megahertz, this sensor has a limited number of elements to ensure a simplicity of design and manufacture for a subsequent miniaturization of the sensor to achieve frequencies near the gigahertz
Moneron, Gael. „Microscopie tridimensionnelle à très haute résolution par tomographie par cohérence optique“. Paris 6, 2006. http://www.theses.fr/2006PA066623.
Der volle Inhalt der QuelleMurtin, Chloé Isabelle. „Traitement d’images de microscopie confocale 3D haute résolution du cerveau de la mouche Drosophile“. Thesis, Lyon, 2016. http://www.theses.fr/2016LYSEI081/document.
Der volle Inhalt der QuelleAlthough laser scanning microscopy is a powerful tool for obtaining thin optical sections, the possible depth of imaging is limited by the working distance of the microscope objective but also by the image degradation caused by the attenuation of both excitation laser beam and the light emitted from the fluorescence-labeled objects. Several workaround techniques have been employed to overcome this problem, such as recording the images from both sides of the sample, or by progressively cutting off the sample surface. The different views must then be combined in a unique volume. However, a straightforward concatenation is often not possible, because the small rotations that occur during the acquisition procedure, not only in translation along x, y and z axes but also in rotation around those axis, making the fusion uneasy. To address this problem we implemented a new algorithm called 2D-SIFT-in-3D-Space using SIFT (scale Invariant Feature Transform) to achieve a robust registration of big image stacks. Our method register the images fixing separately rotations and translations around the three axes using the extraction and matching of stable features in 2D cross-sections. In order to evaluate the registration quality, we created a simulator that generates artificial images that mimic laser scanning image stacks to make a mock pair of image stacks one of which is made from the same stack with the other but is rotated arbitrarily with known angles and filtered with a known noise. For a precise and natural-looking concatenation of the two images, we also developed a module progressively correcting the sample brightness and contrast depending on the sample surface. Those tools we successfully used to generate tridimensional high resolution images of the fly Drosophila melanogaster brain, in particular, its octopaminergic and dopaminergic neurons and their synapses. Those monoamine neurons appear to be determinant in the correct operating of the central nervous system and a precise and systematic analysis of their evolution and interaction is necessary to understand its mechanisms. If an evolution over time could not be highlighted through the pre-synaptic sites analysis, our study suggests however that the inactivation of one of these neuron types triggers drastic changes in the neural network
Khamis, David. „SECM à haute résolution temporelle pour l'étude de processus électrocatalytiques“. Paris 6, 2011. http://www.theses.fr/2011PA066327.
Der volle Inhalt der QuelleJerosolimski, Guillaume. „Imagerie thermique haute résolution de circuits intégrés en fonctionnement par thermoréflectance“. Paris 6, 2005. http://www.theses.fr/2005PA066596.
Der volle Inhalt der QuelleCaillat, Ludovic. „Nano-sondes optiques à forte non-linéarite pour l'imagerie cellulaire à haute résolution“. Paris 6, 2013. http://www.theses.fr/2013PA066059.
Der volle Inhalt der QuelleMajor bottleneck in microscopic imaging is the limited lateral resolution due to the diffraction of light. To overcome this limit, here we demonstrate the up-conversion process in the rare earth doped nanoparticles, which may serve as an original fluorescence source mechanism. Rare earth doped nanoparticles, have been reported to serve as efficient bio-labels for cellular and small animal imaging. In this work, we demonstrate that non-linearity of up-conversion allows achieving high lateral resolution in the images using multiphoton microscopy, demonstrating significant improvement in lateral resolution, using low pumping laser power. This new technique may serve as another approach for high-resolution optical imaging
Fahys, Audrey. „Micro et nano-antennes adaptées à la microscopie champ proche et à l'imagerie haute résolution“. Besançon, 2007. http://www.theses.fr/2007BESA2046.
Der volle Inhalt der QuelleThis work consists of the study and the implementation of a near-field optical microscopy using annular nano-antennas to distinguish electric and magnetic components of the electromagnetic field, detected close to a sample. We present near-field microscopy by advancing the importance of intrinsic characteristics of an illumination system, and also the role of nanocollector in interaction with electromagnetic field. The antennas are one of major stake in the art to detect electromagnetic fields, then we give antennas mapping in various scope frequencies and their respective applications for near-field microscopy. We explain the fabrication in four steps of a metallic annular nano-antenna with specific size at the extremity of an optical fiber. After describing the illumination system and the experimental setup for the optical characterization, we present the study of a new typical probe: an annular nano-antenna at the extremity of fiber micro-axicon. By running the polychromatic polarized Bessel beam properties, we can access to collection properties due to the annular structure. We show that a nano-antenna can selectively collect the longitudinal component of the electric field from a radially polarized beam and the longitudinal component of the magnetic field from an azimuthally polarized beam. Therefore, we can expect a new analyze for electric or magnetic properties of samples in near-field scanning optical microscopy
Bücher zum Thema "Microscopie haute résolutive"
Florian, Banhart, Hrsg. In-situ electron microscopy at high resolution. Hackensack, NJ: World Scientific, 2008.
Den vollen Inhalt der Quelle findenBuchteile zum Thema "Microscopie haute résolutive"
Fredj, A. Ben, und G. Nihottl. „Observation en microscopie électronique à haute résolution d'oxydes à grande maille (c-Dy203) et interprétation des images obtenues“. In October 16, 351. De Gruyter, 1985. http://dx.doi.org/10.1515/9783112500941-005.
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