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1

Shen, Minchong, Jiangang Li, Yuanhua Dong, Zhengkun Zhang, Yu Zhao, Qiyun Li, Keke Dang, Junwei Peng, and Hong Liu. "The Effects of Microbial Inoculants on Bacterial Communities of the Rhizosphere Soil of Maize." Agriculture 11, no. 5 (April 25, 2021): 389. http://dx.doi.org/10.3390/agriculture11050389.

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The bacterial community of rhizosphere soil maintains soil properties, regulates the microbiome, improves productivity, and sustains agriculture. However, the structure and function of bacterial communities have been interrupted or destroyed by unreasonable agricultural practices, especially the excessive use of chemical fertilizers. Microbial inoculants, regarded as harmless, effective, and environmentally friendly amendments, are receiving more attention. Herein, the effects of three microbial inoculants, inoculant M and two commercial inoculants (A and S), on bacterial communities of maize rhizosphere soil under three nitrogen application rates were compared. Bacterial communities treated with the inoculants were different from those of the non-inoculant control. The OTU (operational taxonomic unit) numbers and alpha diversity indices were decreased by three inoculants, except for the application of inoculant M in CF group. Beta diversity showed the different structures of bacterial communities changed by three inoculants compared with control. Furthermore, key phylotypes analyses exhibited the differences of biomarkers between different treatments visually. Overall, inoculant M had shared and unique abilities of regulating bacterial communities compared with the other two inoculants by increasing potentially beneficial bacteria and decreasing the negative. This work provides a theoretical basis for the application of microbial inoculants in sustainable agriculture.
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Li, Chong, Zhaohui Jia, Shilin Ma, Xin Liu, Jinchi Zhang, and Christoph Müller. "Plant and Native Microorganisms Amplify the Positive Effects of Microbial Inoculant." Microorganisms 11, no. 3 (February 24, 2023): 570. http://dx.doi.org/10.3390/microorganisms11030570.

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Microbial inoculants can be used to restore abandoned mines because of their positive effects on plant growth and soil nutrients. Currently, soils in greenhouse pot studies are routinely sterilized to eradicate microorganisms, allowing for better inoculant colonization. Large-scale field sterilization of abandoned mining site soils for restoration is difficult, though. In addition, microbial inoculants have an impact on plants. Plants also have an impact on local microbes. The interactions among microbial inoculants, native microorganisms, and plants, however, have not been studied. We created a pot experiment utilizing the soil and microbial inoculant from a previous experiment because it promoted plant growth in that experiment. To evaluate the effects of the plants, native microorganisms, and microbial inoculants, we assessed several indicators related to soil elemental cycling and integrated them into the soil multifunctionality index. The addition of the microbial inoculant and sterilizing treatment had a significant impact on alfalfa growth. When exposed to microbial inoculant treatments, the plant and sterilization treatments displayed radically different functional characteristics, where most of the unsterilized plant treatment indices were higher than those of the others. The addition of microbial inoculant significantly increased soil multifunctionality in plant treatments, particularly in the unsterilized plant treatment, where the increase in soil multifunctionality was 260%. The effect size result shows that the positive effect of microbial inoculant on soil multifunctionality and unsterilized plant treatment had the most significant promotion effect. Plant and native microorganisms amplify the positive effects of microbial inoculant.
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Liu, Yi-Ming, Fang Zheng, Zhao-Hui Liu, Hai-Bo Lan, Ye-Hong Cui, Tong-Guo Gao, Marja Roitto, and Ai-Fang Wang. "Enhanced Root and Stem Growth and Physiological Changes in Pinus bungeana Zucc. Seedlings by Microbial Inoculant Application." Forests 13, no. 11 (November 4, 2022): 1836. http://dx.doi.org/10.3390/f13111836.

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Background and Objectives: As an extensively used tree species in landscaping and afforestation in China, lacebark pine (Pinus bungeana Zucc.) seedlings are in high demand. However, the small number of fine roots and the low growth rate of lacebark pine seedlings increase the risks encountered during transplant and extend the nursery time for outplanting. We aimed to find out whether a microbial inoculant would promote root growth and accordingly, shorten the nursery cultivation time. Materials and Methods: One-year-old lacebark pine seedlings were treated with the inoculant Bacillus subtilis 8–32 six times from June to September. At each application time, five treatments of undiluted microbial inoculants (UM), 30 times diluted microbial inoculants (30 DM), 40 times diluted microbial inoculants (40 DM), 50 times diluted microbial inoculants (50 DM), and distilled water as a control (CTRL) were administered to the seedlings. In the end, all the seedlings were harvested to measure the root growth, aboveground growth, and the physiological indices. Results: Root and stem growth was enhanced by the inoculants in terms of the increased number of root tips, the length and surface area of the roots, the biomass of the roots and stems, as well as the increase in height and basal stem diameter. The chlorophyll a/b of the needles was increased, in spite of the fact that the total chlorophyll content was decreased by the microbial inoculant treatments at the end of the growth phase. Meanwhile, the maximum photochemical efficiency (Fv/Fm) of the needles was increased by the inoculant treatments. The soluble sugar content was additionally translocated into the stems in the UM treatment, suggesting the change in carbon allocation. The content of available potassium, phosphorus, and ammonium nitrogen in the potting soil was increased in the 30 DM group, and the content of soil organic matter was increased in all the inoculant treatments. Conclusions: The microbial inoculant Bacillus subtilis 8–32, in appropriate concentrations, could be applied to promote root and shoot growth and improve the seedling quality of the lacebark pine during cultivation.
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Broschat, Timothy K., and Monica L. Elliott. "Effects of Fertilization and Microbial Inoculants Applied at Transplanting on the Growth of Mexican Fan Palm and Queen Palm." HortTechnology 19, no. 2 (January 2009): 324–30. http://dx.doi.org/10.21273/hortsci.19.2.324.

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Container-grown mexican fan palm (Washingtonia robusta) and queen palm (Syagrus romanzoffiana) transplanted into a field nursery having phosphorus (P)-sufficient and P-deficient soils were treated at the time of planting with four commercial microbial inoculants (each containing arbuscular mycorrhizal fungi, alone or with other microbial components or fertilizers), two fertilizers, or nothing (control). All but the control palms received applications of an 8N–0.9P–10K palm fertilizer every 3 months for 2 years. None of the treatments improved growth over the control in the P-deficient soil. In the P-sufficient soil, none of the microbial inoculants improved growth over that of similarly fertilized noninoculated palms. Discrepancies were observed regarding nonmycorrhizal fungi and bacteria present in the microbial inoculant products. The type and quantity of these microbes listed on the labels of the microbial inoculant products did not necessarily match the type and quantity actually detected in the products.
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Broschat, Timothy K., and Monica L. Elliott. "Effects of Fertilization and Microbial Inoculants Applied at Transplanting on the Growth of Mexican Fan Palm and Queen Palm." HortTechnology 19, no. 2 (January 2009): 324–30. http://dx.doi.org/10.21273/horttech.19.2.324.

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Container-grown mexican fan palm (Washingtonia robusta) and queen palm (Syagrus romanzoffiana) transplanted into a field nursery having phosphorus (P)-sufficient and P-deficient soils were treated at the time of planting with four commercial microbial inoculants (each containing arbuscular mycorrhizal fungi, alone or with other microbial components or fertilizers), two fertilizers, or nothing (control). All but the control palms received applications of an 8N–0.9P–10K palm fertilizer every 3 months for 2 years. None of the treatments improved growth over the control in the P-deficient soil. In the P-sufficient soil, none of the microbial inoculants improved growth over that of similarly fertilized noninoculated palms. Discrepancies were observed regarding nonmycorrhizal fungi and bacteria present in the microbial inoculant products. The type and quantity of these microbes listed on the labels of the microbial inoculant products did not necessarily match the type and quantity actually detected in the products.
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Calvo, Pamela, Dexter B. Watts, Joseph W. Kloepper, and H. Allen Torbert. "The influence of microbial-based inoculants on N2O emissions from soil planted with corn (Zea maysL.) under greenhouse conditions with different nitrogen fertilizer regimens." Canadian Journal of Microbiology 62, no. 12 (December 2016): 1041–56. http://dx.doi.org/10.1139/cjm-2016-0122.

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Nitrous oxide (N2O) emissions are increasing at an unprecedented rate owing to the increased use of nitrogen (N) fertilizers. Thus, new innovative management tools are needed to reduce emissions. One potential approach is the use of microbial inoculants in agricultural production. In a previous incubation study, we observed reductions in N2O emissions when microbial-based inoculants were added to soil (no plants present) with N fertilizers under laboratory incubations. This present study evaluated the effects of microbial-based inoculants on N2O and carbon dioxide (CO2) emissions when applied to soil planted with corn (Zea mays L.) under controlled greenhouse conditions. Inoculant treatments consisted of (i) SoilBuilder (SB), (ii) a metabolite extract of SoilBuilder (SBF), and (iii) a mixture of 4 strains of plant-growth-promoting Bacillus spp. (BM). Experiments included an unfertilized control and 3 N fertilizers: urea, urea – ammonium nitrate with 32% N (UAN-32), and calcium – ammonium nitrate with 17% N (CAN-17). Cumulative N2O fluxes from pots 41 days after planting showed significant reductions in N2O of 15% (SB), 41% (BM), and 28% (SBF) with CAN-17 fertilizer. When UAN-32 was used, reductions of 34% (SB), 35% (SBF), and 49% (BM) were obtained. However, no reductions in N2O emissions occurred with urea. Microbial-based inoculants did not affect total CO2emissions from any of the fertilized treatments or the unfertilized control. N uptake was increased by an average of 56% with microbial inoculants compared with the control (nonmicrobial-based treatments). Significant increases in plant height, SPAD chlorophyll readings, and fresh and dry shoot mass were also observed when the microbial-based treatments were applied (with and without N). Overall, results demonstrate that microbial inoculants can reduce N2O emissions following fertilizer application depending on the N fertilizer type used and can enhance N uptake and plant growth. Future studies are planned to evaluate the effectiveness of these microbial inoculants in field-based trials and determine the mechanisms involved in N2O reduction.
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Vendramini, Joao M. B., and Philipe Moriel. "151 Microbial inoculant effects on cool- and warm-season grass silage." Journal of Animal Science 102, Supplement_3 (September 1, 2024): 392. http://dx.doi.org/10.1093/jas/skae234.445.

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Abstract Silage has been the preferred method of forage conservation in many regions of the world, primarily due to frequent rainfall during the period of maximum forage growth and limited opportunities to preserve forage as hay. Corn and sorghum are the main forages conserved as silage, but cultivation of annual summer crops is costly and may be risky due to variable climactic conditions. Therefore, livestock producers have relied on perennial grasses as a source of forage for conservation as silage. However, most grasses have undesirable characteristics that reduce the potential of achieving satisfactory fermentation and conservation, such as limited dry matter concentration, and morphological and chemical characteristics. Several management practices have been developed with the objective of improving grass silage fermentation and nutritive value, and microbial inoculants have been among the most used management practices. Nonetheless, most microbial inoculants have been tested and developed for corn silage and later recommended to grass silage with limited scientific evidence for the efficacy of the specific inoculant. The most used grasses as forages are divided into cool- (C3) and warm-season (C4) perennial grasses with notorious differences between them. Cool-season grasses have greater water-soluble carbohydrates and lesser structural carbohydrates concentrations than warm-season perennial grasses, which are desirable traits for better silage fermentation and positive microbial inoculant effects. Warm-season perennial grasses have greater accumulation of starch as non-structural carbohydrate, which does not directly contribute to silage fermentation. In addition to the limitations mentioned previously, warm-season perennial grasses have decreased dry matter concentration and nutritive value, reducing the likelihood of achieving desirable fermentation. A literature review was conducted to evaluate the effects of microbial inoculant on nutritive value and fermentation characteristics (pH, lactic acid, acetic acid, butyric acid, and ammonia concentrations) of grass silage. In addition, the effect of different microbial inoculants (homofermentative vs. heterofermentative) was evaluated. Lastly, results from current research projects testing the effects of microbial inoculants on silage microbiome and gas production were presented.
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Pabar, Sándor Attila, Zsolt Kotroczó, Tünde Takács, and Borbála Biró. "Evaluating the Efficacy of Selected Plant Growth-Promoting Microorganisms in Optimizing Plant Growth and Soil Health in Diverse Soil Types." Agriculture 14, no. 9 (September 12, 2024): 1586. http://dx.doi.org/10.3390/agriculture14091586.

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This study explores the efficacy of bio-efficient solutions, specifically plant growth-promoting microorganisms (PGPMs), in sustainable soil management. This research was conducted in 2020. It evaluates the impact of various single microbial inoculants, including Enterobacter ludwigii, Bacillus subtilis, Pseudomonas fluorescens, Kosakonia cowanii, and Trichoderma harzianum, on plant growth soil enzyme activity and organism abundance. Perennial ryegrass and mustard were used as test plants, in controlled environmental conditions. The results show generally positive effects of microbial inoculants on plant biomass (E. ludwigii increased ryegrass biomass by 9.75%, and P. fluorescens increased mustard biomass by up to 38.81% compared to the control) and on soil microbial activities. Our study further investigated the combined application of all these strains in five different soil types and textures. The results highlight the significance of soil physicochemical properties in determining inoculant efficacy; we found that clayey soils with higher colloid content support more robust microbial activity. Additionally, using natural clay minerals like alginite for enhancing soil conditions showed promising interactions with microbial inoculants, although application requires further optimization. These findings suggest that integrating microbial inoculants in sustainable agricultural practices could enhance plant growth, improve soil health, and reduce the need of chemical fertilizers. Future research should aim to refine the combinations and application methods of these bio-efficient solutions for broader agricultural applicability.
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Prischmann-Voldseth, Deirdre A., Tülin Özsisli, Laura Aldrich-Wolfe, Kirk Anderson, and Marion O. Harris. "Microbial Inoculants Differentially Influence Plant Growth and Biomass Allocation in Wheat Attacked by Gall-Inducing Hessian Fly (Diptera: Cecidomyiidae)." Environmental Entomology 49, no. 5 (August 29, 2020): 1214–25. http://dx.doi.org/10.1093/ee/nvaa102.

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Abstract Beneficial root microbes may mitigate negative effects of crop pests by enhancing plant tolerance or resistance. We used a greenhouse experiment to investigate impacts of commercially available microbial root inoculants on growth and biomass allocation of wheat (Triticum aestivum L. [Cyperales: Poaceae]) and on survival and growth of the gall-inducing wheat pest Hessian fly, Mayetiola destructor (Say). A factorial design was used, with two near-isogenic wheat lines (one susceptible to Hessian fly, the other resistant), two levels of insect infestation (present, absent), and four inoculants containing: 1) Azospirillum brasilense Tarrand et al. (Rhodospirillales: Azospirillaceae), a plant growth-promoting bacterium, 2) Rhizophagus intraradices (N.C. Schenck & G.S. Sm.) (Glomerales: Glomeraceae), an arbuscular mycorrhizal fungus, 3) A. brasilense + R. intraradices, and 4) control, no inoculant. Larval feeding stunted susceptible wheat shoots and roots. Plants had heavier roots and allocated a greater proportion of biomass to roots when plants received the inoculant with R. intraradices, regardless of wheat genotype or insect infestation. Plants receiving the inoculant containing A. brasilense (alone or with R. intraradices) had comparable numbers of tillers between infested and noninsect-infested plants and, if plants were susceptible, a greater proportion of aboveground biomass was allocated to tillers. However, inoculants did not impact density or performance of Hessian fly immatures or metrics associated with adult fitness. Larvae survived and grew normally on susceptible plants and mortality was 100% on resistant plants irrespective of inoculants. This initial study suggests that by influencing plant biomass allocation, microbial inoculants may offset negative impacts of Hessian flies, with inoculant identity impacting whether tolerance is related to root or tiller growth.
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Zhang, Kaizheng. "Production of Synthetical Microbial Inoculant for Low-Temperature Daqu Based on Their Core Functional Microflora." Food Science and Nutrition 9, no. 4 (December 31, 2023): 1–9. http://dx.doi.org/10.24966/fsn-1076/100169.

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By summarizing the core functional microbial flora of low-temperature Daqu, 16 strains belonging to 13 genera were obtained for the preparation of low-temperature Daqu microbial inoculant. In the production of initial microbial inoculants, the core microorganisms were first activated and expanded, and the appropriate centrifugation and concentration conditions were determined by multiple groups of parallel experiments.
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Luo, Zhengyu, Hongrui Han, Hui Yao, Guoru Yan, Jinxin Bai, Lihao Shi, Xiangjun Pei, Jingji Li, and Qiang Li. "Effects of Artificially Modified Microbial Communities on the Root Growth and Development of Tall Fescue in Nutrient-Poor Rubble Soil." Plants 13, no. 23 (November 25, 2024): 3307. http://dx.doi.org/10.3390/plants13233307.

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The granite rubble soil produced through excavation during construction is nutrient-poor and has a simplified microbial community, making it difficult for plants to grow and increasing the challenges of ecological restoration. Recent studies have demonstrated that microbial inoculants significantly promote plant growth and are considered a potential factor influencing root development. Microorganisms influence root development either directly or indirectly, forming beneficial symbiotic relationships with plant roots. However, the mechanisms by which microorganisms affect root development and root anatomy, as well as the dynamics of soil microbial communities following the artificial application of microbial inoculants, remain unclear. This experiment utilized granite rubble soil from construction excavation in a pot trial, implementing five different treatment methods. After the fast-growing grass species tall fescue (Festuca arundinacea) was planted, four growth-promoting microbial inoculants—Bacillus subtilis (K), Bacillus amyloliquefaciens (JD), Aspergillus niger (H), and Trichoderma harzianum (HC)—were applied to the soil in the pots. These treatments were compared with a control group (CK) that received no microbial inoculant. At 120 days of plant growth, the composition of the soil microbial community, biomass, root structure, and root anatomy were measured for each treatment group. This analysis aimed to explore the effects of different microbial treatments on the microbial communities and root development of Festuca arundinacea root soil. The study found that the addition of microbial inoculants reduced the number of microbial operational taxonomic units (OTUs) of bacteria and fungi in the soil, affecting both the marker species and their abundance at the phylum level. Additionally, microbial inoculants promoted the development of the tall fescue root structure, increasing metrics such as the total root length, root surface area, root volume, and root-to-shoot ratio per plant. Redundancy analysis (RDA) revealed that the area ratios of various components in the root anatomy of tall fescue’s primary roots, such as the root cortex area, stele area, and the number of lateral roots, were influenced by Proteobacteria. Mortierellomycota was found to affect the root epidermis area.
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He, Shanmu, Ying Zhang, Xiaolei Yang, Qi Li, Changning Li, and Tuo Yao. "Effects of Microbial Inoculants Combined with Chemical Fertilizer on Growth and Soil Nutrient Dynamics of Timothy (Phleum pratense L.)." Agronomy 14, no. 5 (May 10, 2024): 1016. http://dx.doi.org/10.3390/agronomy14051016.

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Microbial inoculants derived from plant growth-promoting rhizobacteria (PGPR) offer eco-friendly alternatives to traditional chemical fertilizers, maintaining microbiota balance in agricultural systems. However, limited research has explored the combined effects of microbial inoculants and chemical fertilizers on crop growth and soil properties. In this study, we investigated seven fertilizer combinations, ranging from no fertilizer to various proportions of chemical fertilizers with microbial inoculants, on timothy (Phleum pratense L.) growth, chlorophyll content, soil properties, enzyme activities, and soil microbial communities. A randomized block design was employed to analyze these effects. The results indicate that the combination of 85% chemical fertilizer with microbial inoculants significantly increased timothy yield and chlorophyll content. In addition, a reduction to 55% chemical fertilizer in conjunction with microbial inoculants resulted in comparable yield to that of 100% fertilizer with no inoculants. The microbial inoculants treatments notably elevated soil catalase, urease, acid phosphatase, and invertase activities, along with soil fast-acting nutrient content. The sequencing results show that the abundance of beneficial bacteria increased, while that of fungi decreased in the soil rhizosphere after the application of microbial inoculants. This study underscored the potential of microbial inoculants combined with reductions in chemical fertilizers to enhance soil microbiology, nutrient content, and beneficial microbial abundance while suppressing pathogenic fungi, thereby promoting timothy growth and yield. These findings provide a theoretical basis for the use of microbial inoculants in sustainable agricultural practices, providing valuable insights for optimizing microbial inoculants and chemical fertilizer formulations to mitigate the sustainability challenges posed by conventional fertilizers.
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Ma, Hua, Vyacheslav Shurigin, Dilfuza Jabborova, Jeane Aril dela Cruz, Thomas Edison dela Cruz, Stephan Wirth, Sonoko Dorothea Bellingrath-Kimura, and Dilfuza Egamberdieva. "The Integrated Effect of Microbial Inoculants and Biochar Types on Soil Biological Properties, and Plant Growth of Lettuce (Lactuca sativa L.)." Plants 11, no. 3 (February 3, 2022): 423. http://dx.doi.org/10.3390/plants11030423.

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Numerous reports confirm the positive effect of biochar application on soil properties and plant development. However, the interaction between root-associated beneficial microbes and different types of biochar is not well understood. The objective of this study was to evaluate the plant growth of lettuce after the application of three types of biochar in loamy, sandy soil individually and in combination with plant-beneficial microbes. Furthermore, total microbial activity in rhizosphere soil of lettuce was measured by means of fluorescein diacetate (FDA) hydrolase and enzyme activities linked to carbon, nitrogen, and phosphorus cycling. We used three types of biochar: (i) pyrolysis char from cherry wood (CWBC), (ii) pyrolysis char from wood (WBC), and (iii) pyrolysis char from maize (MBC) at 2% concentration. Our results showed that pyrolysis biochars positively affected plant interaction with microbial inoculants. Plant dry biomass grown on soil amended with MBC in combination with Klebsiella sp. BS13 and Klebsiella sp. BS13 + Talaromyces purpureogenus BS16aPP inoculants was significantly increased by 5.8% and 18%, respectively, compared to the control plants. Comprehensively, interaction analysis showed that the biochar effect on soil enzyme activities involved in N and P cycling depends on the type of microbial inoculant. Microbial strains exhibited plant growth-promoting traits, including the production of indole 3-acetic-acid and hydrogen cyanide and phosphate-solubilizing ability. The effect of microbial inoculant also depends on the biochar type. In summary, these findings provide new insights into the understanding of the interactions between biochar and microbial inoculants, which may affect lettuce growth and development.
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Sharma, A. K., and P. N. Bhattacharyya. "Effect of Beneficial Microorganisms on Cowpea Productivity and Soil Health." Journal of Advance Research in Pharmacy & Biological Science (ISSN: 2208-2360) 2, no. 5 (May 31, 2016): 15–21. http://dx.doi.org/10.53555/nnpbs.v2i5.702.

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Soil microorganisms are the most abundant biota in soil, responsible for a number of abilities such as nutrient cycling and organic matter decomposition, maintenance of soil fertility and restoration and plant health and sustainability in ecosystem functioning. Beneficial microbial inoculants such as actinomycetes, diazotrophic bacteria, mycorrhizal helper bacteria (MHB), mycorrhizal fungi, rhizobia etc. are known to promote plant growth. Microorganisms are also antagonistic to plant pests, parasites or diseases. Many of the beneficial microbials are naturally present in soil, although in certain cases, it may be advised to increase their populations and activity either through direct inoculation or by applying agricultural management techniques. In cognizance with the above, an experiment was conducted to evaluate the effect of microbial inoculants on overall productivity of cowpea and soil health. Results revealed that the application of Rhizobium sp. as seed treatment increased the productivity of cowpea (up to 15%) at various stages of plant growth parameters like plant dry weight, no. of fresh leaves and branches, pods, overall leaf moisture and root length as compared to control. Total microbial population numbers, available K and phosphorus (P) in soil were also increased significantly after the soil was treated with this microbial inoculant indicating the role of beneficial microbial in improving the plant nutrient status and soil health.
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Adesemoye, A. O., H. A. Torbert, and J. W. Kloepper. "Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system." Canadian Journal of Microbiology 54, no. 10 (October 2008): 876–86. http://dx.doi.org/10.1139/w08-081.

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A 3 year field study was conducted with field corn from 2005 to 2007 to test the hypothesis that microbial inoculants that increase plant growth and yield can enhance nutrient uptake, and thereby remove more nutrients, especially N, P, and K from the field as part of an integrated nutrient management system. The field trial evaluated microbial inoculants, which include a commercially available plant growth-promoting rhizobacteria (PGPR), arbuscular mycorrhiza fungi (AMF), and their combination across 2 tillage systems (no-till and conventional till) and 2 fertilization regimes (poultry litter and ammonium nitrate). Data were collected on plant height, yield (dry mass of ears and silage), and nutrient content of corn grain and silage. In addition, nutrient content of soil was determined, and bioavailability of soil nutrient was measured with plant root simulator probes. Results showed that inoculants promoted plant growth and yield. For example, grain yields (kg·ha–1) in 2007 for inoculants were 7717 for AMF, 7260 for PGPR+AMF, 7313 for PGPR, 5725 for the control group, and for fertilizer were 7470 for poultry litter and 6537 for NH4NO3. Nitrogen content per gram of grain tissues was significantly enhanced in 2006 by inoculant, fertilizer, and their interactions. Significantly higher amounts of N, P, and K were removed from the plots with inoculants, based on total nutrient content of grain per plot. These results supported the overall hypothesis and indicate that application of inoculants can lead to reduction in the build up of N, P, and K in agricultural soils. Further studies should be conducted to combine microbial inoculants with reduced rates of fertilizer.
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Raja, P., and V. P. Santhi. "Comparative study of microbial inoculants of cultivated and virgin soils of Nilgiri Biosphere for plant growth promotion." INTERNATIONAL JOURNAL OF AGRICULTURAL SCIENCES 17, no. 2 (June 15, 2021): 293–98. http://dx.doi.org/10.15740/has/ijas/17.2/293-298.

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In virgin soils, microorganisms and plants live in harmony and both are dependent on each other for their livelihood. Absence of cultivation practices, undisturbed soil condition, high organic matter condition and other favourable conditions enables to flourish beneficial microbes. The research work was started to identify beneficial microbes from undisturbed virgin soils of Nilgiri biosphere with the ability to grow under low pH and under low temeperature conditions. Bio-inoculants viz., Azospirillum, Phosphobacteria, Azotobacter, Rhizobium and pseudomonas were obtained from cultivated and virgin soil samples of Nilgiris biosphere. When compared with type cultures, virgin soil isolates of respective inoculants have recorded better results in promoting plant dry weight in paper towel method. In cross streak assay, selected isolates found to be compatible with each other. In lignite carrier base formulation, the inoculants have reached a maximum population level of 107 and phosphobacteria reached 108 level. The population remained steady at this level up to 3 months. In the field trial studies conducted, the treatment of Azospirillum + Azotobacter + Phosphobacteria + Pseudomonas + 75% RDF has recorded maximum population of all the inoculants at 45th day after sowing. However, the maximum yield was observed in 100% RDF and bio-inoculant consortium applied treatment. This was closely followed by 75% RDF and bio-inoculant consortium applied plots. The results of the field trial have shown that bio-inoculant consortium along with 75% RDF application will lead to maximum yield with 25% saving in chemical fertilizer application.
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Oliveira, Andréia de, Marcelo Akira Saito, Alessandra Guedes Baleroni, Robson Akira Matsuzaki, Filipe Bertagna, Amanda Tami Kuroda Colevate, Carlos Alberto Scapim, and Leandro Simoes Azevedo Gonçalves. "Methods of inoculation of plant growth-promoting rhizobacteria in specialty maize genotypes under organic agriculture system." Acta Scientiarum. Agronomy 44 (May 24, 2022): e54910. http://dx.doi.org/10.4025/actasciagron.v44i1.54910.

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Organic agriculture systems have the nutrients supplied by plant or animal by-products, bioinoculants, and compost-based products as earthworm composts and green manures. However, the quantitative and qualitative parameters of soil amendments depend on their sources, and soil amendments are generally not sufficient to supply the nutritional requirements of maize crops. Moreover, specialty maize requires high levels of N. Thus, the aim of this study was to investigate specialty maize varieties supplied with two microbial inoculants applied in two inoculation methods. These factorial treatments were compared with their checks (varieties without inoculation), and the interaction among these factors was also investigated. The trials were carried out during the growing season in 2017–2018 in the State University of Maringá. The popcorn trial followed the randomized complete block design where the factorial 3 × 2 × 2 + 3 had five replications. The trial with white grits maize followed the same experimental design but the factorial scheme was 2 × 2 × 2 + 2 with three replications. Both trials had maize varieties and two species of microbial inoculants (Azospirillum brasilense and Methylobacterium sp.) applied in two inoculation methods, in the seeds and the foliar spray at V4 stage of plant development. The response traits were grain yield and the components of crop production. In both trials, we verified that the majority of the interactions among the factors was non-significant (p > 0.05), indicating the independence of these factors. Furthermore, the microbial inoculants had no beneficial effects on the traits. The possibility of a higher crop yield did not confirm the application of the inoculant in the stage V4. The organic compost may be the key point in mitigating the treatments with microbial inoculants due to the availability of N in the first stages of plant development. The traits also suggest the necessity of more trials about the influence of microbial inoculants on specialty maize production.
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Loredana, Canfora, Costa Corrado, Pallottino Federico, and Mocali Stefano. "Trends in Soil Microbial Inoculants Research: A Science Mapping Approach to Unravel Strengths and Weaknesses of Their Application." Agriculture 11, no. 2 (February 16, 2021): 158. https://doi.org/10.3390/agriculture11020158.

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Microbial inoculants are widely accepted as potential alternatives or complements to chemical fertilizers and pesticides in agriculture. However, there remains a lack of knowledge regarding their application and effects under field conditions. Thus, a quantitative description of the scientific literature related to soil microbial inoculants was conducted, adopting a science mapping approach to observe trends, strengths, and weaknesses of their application during the period of 2000–2020 and providing useful insights for future research. Overall, the study retrieved 682 publications with an increasing number during the 2015–2020 period, confirming China, India, and the U.S. as leading countries in microbial inoculants research. Over the last decade, the research field emphasized the use of microbial consortia rather than single strains, with increasing attention paid to sustainability and environmental purposes by means of multidisciplinary approaches. Among the emerging topics, terms such as “persistence” indicate the actual need for detecting and monitoring the persistence and fate of soil microbial inoculants. On the other hand, the low occurrence of terms related to failed studies as well as formulation processes may have limited the overall comprehension of the real potential of microbial inoculants to date. In conclusion, successful application of soil microbial inoculants in agriculture requires filling the fundamental knowledge gaps related to the processes that govern dynamics and interactions of the inoculants with soil and its native microbiota
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Ferraretto, Luiz. "155 Modulating silage fermentation with microbial inoculants." Journal of Animal Science 102, Supplement_3 (September 1, 2024): 386–87. http://dx.doi.org/10.1093/jas/skae234.439.

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Abstract Silage is a vital feed ingredient for beef and dairy cattle diets in many areas worldwide. Even though ensiling is a well-established method of forage preservation, producers face a myriad of challenges to produce, harvest, and store forages optimally. Challenging circumstances like these create difficulties for proper silage fermentation, increase dry matter (DM) losses and silage contamination with mycotoxins, and reduce the nutritive value of ensiled forages. Losses of DM and nutrients occur during production, fermentation, and feeding of silage. Although some losses are unavoidable, poor management may increase DM losses up to 60% which may eventually lead to decreased feed efficiency, as each percentage-unit lost as DM is a percentage-unit of feed that cannot be converted into animal products. Loss of DM during forage production and preservation is estimated to cost beyond a billion dollars worldwide. Even in well-managed silages, yeasts and molds that survive anaerobic fermentation may grow rapidly and reduce aerobic stability after silo opening, and mold count in silage is positively related to DM loss. The use of silage microbial inoculants is a key strategy to modulate silage fermentation and preserve forage successfully. This presentation will discuss microbial inoculant strategies to optimize silage fermentation and minimize silage losses. Homofermentative bacterial inoculants, which contain bacteria that almost exclusively produce lactic acid, have been shown to effectively reduce silage pH and improve DM recovery. The use of homofermentative microbial inoculants have been shown to attenuate DM loss during ensiling and to increase dry matter intake and hence lactation performance by dairy cows. Heterofermentative bacterial inoculants, which contain bacteria capable of producing both lactic and acetic acids, reduce mold and yeast counts and improve silage aerobic stability.
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MATOS, ANABELLE, and JAY L. GARLAND. "Effects of Community Versus Single Strain Inoculants on the Biocontrol of Salmonella and Microbial Community Dynamics in Alfalfa Sprouts†." Journal of Food Protection 68, no. 1 (January 1, 2005): 40–48. http://dx.doi.org/10.4315/0362-028x-68.1.40.

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Potential biological control inoculants, Pseudomonas fluorescens 2-79 and microbial communities derived from market sprouts or laboratory-grown alfalfa sprouts, were introduced into alfalfa seeds with and without a Salmonella inoculum. We examined their ability to inhibit the growth of this foodborne pathogen and assess the relative effects of the inoculants on the alfalfa microbial community structure and function. Alfalfa seeds contaminated with a Salmonella cocktail were soaked for 2 h in bacterial suspensions from each inoculant tested. Inoculated alfalfa seeds were grown for 7 days and sampled during days 1, 3, and 7. At each sampling, alfalfa sprouts were sonicated for 7 min to recover microflora from the surface, and the resulting suspensions were diluted and plated on selective and nonselective media. Total bacterial counts were obtained using acridine orange staining, and the percentage culturability was calculated. Phenotypic potential of sprout-associated microbial communities inoculated with biocontrol treatments was assessed using community-level physiological profiles based on patterns of use of 95 separate carbon sources in Biolog plates. Community-level physiological profiles were also determined using oxygen-sensitive fluorophore in BD microtiter plates to examine functional patterns in these communities. No significant differences in total and mesophilic aerobe microbial cell density or microbial richness resulting from the introduction of inoculants on alfalfa seeds with and without Salmonella were observed. P. fluorescens 2-79 exhibited the greatest reduction in the growth of Salmonella early during alfalfa growth (4.22 log at day 1), while the market sprout inoculum had the reverse effect, resulting in a maximum log reduction (5.48) of Salmonella on day 7. Community-level physiological profiles analyses revealed that market sprout communities peaked higher and faster compared with the other inoculants tested. These results suggest that different modes of actions of single versus microbial consortia biocontrol treatments may be involved.
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Chernyuk, S., V. Bomko, A. Zagorodnii, O. Chernyavskyy, M. Slomchynskyy та S. Babenko. "Ефективність відгодівлі молодняку великої рогатої худоби за використання силосу, законсервованого біологічним інокулянтом". Ukrainian Journal of Ecology 7, № 4 (28 грудня 2017): 583–88. http://dx.doi.org/10.15421/2017_164.

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<p>There has been the impact on the corn silage harvesting and the influence of the corn silage store technology of the microbial inoculants studied. The main advantages and the prospects of the silage inoculants using were outlined. This has been the supplements of microorganism’s inhibition impact on the molds and the fungi development established. Thereby, this was possible to provide the initial properties of raw materials preservation. The use of the 11C33 inoculants at a dose of1 gramper 1 tone of the silage during the storage period provides the dry matter reduction at a rate of 6.9 % versus 14.9 % in comparison with the control group respectively. According to the requirements of the GOST 4782:2007 and the results of the biochemical silage studies the harvested feed without the inoculants using can be classified as the 3rd class. However, the treated silage with the 11C33 inoculant can be classified as the 1st class. The biological conservant inoculation during the milky-wax stage of ripeness helps the feed nutrients to have the better preservation. And the corn silage feeding as a part of its ration is likely to increase the average bulls daily weight gain for the feed costs production reduction. The microbial inoculants using during the corn silage harvesting helped to reduce the feed costs with the1 kgof the bull’s live weight gain by 6.07 %. We found that the preslaughter live weight at the age of 15 months was characterized by bulls of the trial group of the Ukrainian black and white milk breed and they were raised on the corn silage with the microbial inoculants. These bulls showed the better results comparing to the control group by 4.3 % (P<0.05) and the bull’s carcass yield of the trial group was higher than the control one, and it was 56.4 %.</p>
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Rajasekar, Kuppuraj, Thilagavathy Daniel, and Natchimuthu Karmegam. "Microbial Enrichment of Vermicompost." ISRN Soil Science 2012 (March 8, 2012): 1–13. http://dx.doi.org/10.5402/2012/946079.

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The present study has been conducted to explore the possibility of enrichment of vermicompost with microbial inoculants (i.e., biofertilizer organisms), Azospirillum brasilense and Rhizobium leguminosarum, optimization of inoculum level, and time of inoculation during vermicomposting. The survival rate of each microbial inoculant, total microbial population in vermicompost, and their correlation with the microbial inoculants during the storage period (180 days) were assessed. The change in population of A. brasilense and R. leguminosarum in vermicompost (at 30, 35, and 40 mL/175 g substrates) with reference to storage period showed highly significant negative correlation (). The total microbial population in A. brasilense and R. leguminosarum inoculated vermicompost was high during initial phases of storage and then total microbial population declined towards the end. The inoculum level of A. brasilense and R. leguminosarum at 35 mL per 175 g vermibed substrate is sufficient to maintain viable cells up to 160 days after ther harvesting of vermicompost. The inoculum of these two biofertilizer organisms into vermibed on the 30th day showed increased survival rate and, hence, the optimized inoculation of 35 mL of inoculum per 175 g substrate on the 30th day of vermicomposting is helpful for the maintenance of sufficient viable population for more than five months in the enriched vermicompost.
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Canfora, Loredana, Corrado Costa, Federico Pallottino, and Stefano Mocali. "Trends in Soil Microbial Inoculants Research: A Science Mapping Approach to Unravel Strengths and Weaknesses of Their Application." Agriculture 11, no. 2 (February 16, 2021): 158. http://dx.doi.org/10.3390/agriculture11020158.

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Microbial inoculants are widely accepted as potential alternatives or complements to chemical fertilizers and pesticides in agriculture. However, there remains a lack of knowledge regarding their application and effects under field conditions. Thus, a quantitative description of the scientific literature related to soil microbial inoculants was conducted, adopting a science mapping approach to observe trends, strengths, and weaknesses of their application during the period of 2000–2020 and providing useful insights for future research. Overall, the study retrieved 682 publications with an increasing number during the 2015–2020 period, confirming China, India, and the U.S. as leading countries in microbial inoculants research. Over the last decade, the research field emphasized the use of microbial consortia rather than single strains, with increasing attention paid to sustainability and environmental purposes by means of multidisciplinary approaches. Among the emerging topics, terms such as “persistence” indicate the actual need for detecting and monitoring the persistence and fate of soil microbial inoculants. On the other hand, the low occurrence of terms related to failed studies as well as formulation processes may have limited the overall comprehension of the real potential of microbial inoculants to date. In conclusion, successful application of soil microbial inoculants in agriculture requires filling the fundamental knowledge gaps related to the processes that govern dynamics and interactions of the inoculants with soil and its native microbiota.
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Leal, Aline Jaime, Edmo Montes Rodrigues, Patrícia Lopes Leal, Aline Daniela Lopes Júlio, Rita de Cássia Rocha Fernandes, Arnaldo Chaer Borges, and Marcos Rogério Tótola. "Microbial inoculants produced from solid waste compost for bioremediation of diesel-contaminated soils." Boletim do Museu Paraense Emílio Goeldi - Ciências Naturais 14, no. 2 (August 27, 2019): 233–44. http://dx.doi.org/10.46357/bcnaturais.v14i2.177.

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A atividade microbiana pode ser estimulada a promover a remoção de hidrocarbonetos no solo ao se introduzir microrganismos hidrocarbonoclásticos. Inoculantes microbianos foram produzidos utilizando-se composto de lixo sólido municipal (CLSM) para a biorremediação de solos contaminados com óleo diesel. A aplicação de diesel ocorreu a cada quatro dias para o inoculante A e a cada oito para o inoculante B. Análises respirométricas, contagem de bactérias heterotróficas totais e avaliação de hidrocarbonetos totais do petróleo (HTP) foram realizadas. Os inoculantes foram avaliados imediatamente após a produção e o armazenamento, à temperatura ambiente e sob refrigeração. A degradação de HTP após 20 dias foi maior na concentração de 30 g/kg. Nesta concentração, a biodegradação de HTP permaneceu entre 98,3 e 99,4%. Após armazenamento, a eficiência do inoculante A foi de 96,5% de degradação (temperatura ambiente) e 98,1% (sob refrigeração). O inoculante B apresentou significativa redução de eficiência após armazenamento, especialmente à temperatura ambiente. A adição de inoculantes aumentou significativamente a densidade de bactérias cultiváveis no solo contaminado com diesel, mesmo após armazenamento. O uso de CLSM na dose de 30 g/kg foi uma estratégia efetiva para a biorremediação de solos contaminados com diesel, permitindo a eliminação de mais de 99% dos contaminantes em 20 dias.
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R, Lakshmipathy, Tejaswi T., Venugopala Rao R., and Bagyaraj D.J. "Soil microbial population as influenced by different crops and the same crop grown in different locations of Paderu, Andhra Pradesh." JOURNAL OF SOIL BIOLOGY AND ECOLOGY 43, no. 2 (September 26, 2023): 70–76. http://dx.doi.org/10.58682/jsbe.43.2/jogy3732.

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Microbial population of soil varies depending on the soil type, climatic conditions, cropping pattern and different cultural practices followed. In the present study, the microbial inoculants with various PGPR were distributed to the farmers of different tribal hamlets. After application of these inoculants, the sampling was made during different crop growth period and microbial population was determined. In general the microbial population was very much improved in the soils of the fields where microbial inoculants were applied. The total microbial population and PGPR viz, Rhizobium, Azospirillum, Azotobacter, PSB, KSB and Pantoea dispersa varied from place to place and in different crops. However, the population was more in plantation crop than other annual crops. In annual crops, the microbial population was more during flowering stage than initial and harvesting stage. This study clearly showed improved microbial population due to application of microbial inoculants in different crops over uninoculated control plots and also more microbial population in plantation crop than in annual crops.
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Bozhinova, Radka. "Investigation on the effect of broomrape infection and mycorrhizal inoculants on mineral composition of oriental tobacco." Bulgarian Journal of Soil Science, Agrochemistry and Ecology 57, no. 3 (September 25, 2023): 38–47. http://dx.doi.org/10.61308/majb3436.

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The influence of broomrape infection and microbial inoculants (Rhizo-Vam Basic®, Mycoplant® and Funky Fungi®) on the mineral composition of oriental tobacco was studied in a pot experiment. Eight treatments were tested, distributed in the following four groups: (1) control; (2) treatment with broomrape infection; (3) treatments with mycorrhizal inoculants and (4) treatments with broomrape+mycorrhizal inoculants. Broomrape infection reduced the dry weight of tobacco leaves by 31.4% compared to the control. A tendency towards a decrease in the concentrations of the essential nutritional elements – P, K, Zn and Cu, and a more pronounced increase in the concentrations of Ca and Mg in the leaves of tobacco infected with broomrape was found. Nutrient removal by infected tobacco leaves was also reduced, especially by removing phosphorus, zinc and copper. Microbial inoculants containing arbuscular mycorrhizal fungi increased the content of mineral nitrogen, available phosphorus and potassium in the soil. Application of mycorrhizal inoculants, in most cases, increased the phosphorus concentration in tobacco leaves - by 18.2%-22.7% compared to the control. This was associated with an improvement in N concentrations (+1.1%-13.5%), K concentrations (+4.3%-32.2%), and Zn concentrations (+9.8%-21.1%) in leaves of inoculated plants. Mycorrhizal inoculants did not prevent biomass reduction of broomrape-infected plants, but partially reduced weight losses. The application of a mycorrhizal inoculant did not completely prevent the decrease in the essential nutrient concentration in the plants infected with broomrape, but it improved the mineral balance and had a positive effect on nitrogen and phosphorus nutrition.
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Buntic, Aneta, Olivera Stajkovic-Srbinovic, Magdalena Knezevic, Djordje Kuzmanovic, Natasa Rasulic, and Dusica Delic. "Development of liquid rhizobial inoculants and pre-inoculation of alfalfa seeds." Archives of Biological Sciences 71, no. 2 (2019): 379–87. http://dx.doi.org/10.2298/abs181008062b.

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Application of liquid microbial inoculants on legume seeds is a sustainable agricultural practice that can improve plant nutrient uptake and increase crop productivity. Inoculants should provide long-term survival of rhizobia in the final product and after application, to legume seeds. Ten different medium formulations of microbial inoculants were examined (yeast mannitol broth with the addition of agar, sodium-alginate, calcium chloride, glycerol or ferric chloride and combinations thereof) for the survival of the efficient nitrogen-fixing rhizobium, Sinorhizobium (Ensifer) meliloti L3Si strain. The most suitable liquid inoculant for survival of L3Si during a storage time of 150 days was the medium formulation containing glycerol in combination with agar or sodium-alginate. Alfalfa seeds were pre-inoculated with four formulations (yeast mannitol broth (YMB), YMB with agar (1 g L-1), YMB with 1 or 5 g L-1 sodium-alginate) for up to three months. Seeds pre-inoculated and stored for one month produced successful alfalfa plants. The nitrogen content in alfalfa obtained from pre-inoculated seeds one month before sowing was adequate and ranged from 3.72-4.19%. Using S. meliloti-based liquid inoculants for alfalfa and application of the pre-inoculation technique can increase the quality of alfalfa crops and reduce cultivation cost.
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Nikolaidou, Charitini, Nikolaos Monokrousos, Pantelitsa D. Kapagianni, Michael Orfanoudakis, Triantafyllia Dermitzoglou, and Efimia M. Papatheodorou. "The Effect of Rhizophagus irregularis, Bacillus subtilis and Water Regime on the Plant–Microbial Soil System: The Case of Lactuca sativa." Agronomy 11, no. 11 (October 29, 2021): 2183. http://dx.doi.org/10.3390/agronomy11112183.

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Inoculation with beneficial microbes represents a promising solution for sustainable agricultural production; however, knowledge on the effects of inoculants on the indigenous microbial communities remains limited. Here, we evaluated the impact of the arbuscular mycorrhizal fungus Rhizophagus irregularis and the promoting rhizobacterium Bacillus subtilis on the growth of Lactuca sativa. The biomass, the composition, and the enzyme activity (urease, acid phosphatase, and β-glycosidase) of the rhizosphere microbial community at two soil moisture levels (5 and 10% soil water content) were evaluated. Fungal colonization was lower in co-inoculated plants than those only inoculated with R. irregularis. Plant growth was enhanced in co-inoculated and B. subtilis inoculated soils. Bacterial biomass and the composition of the microbial communities responded to the joint effect of inoculant type × water regime while the biomass of the other microbial groups (fungi, actinomycetes, microeukaryotes) was only affected by inoculant type. Co-inoculation enhanced the activity of acid phosphatase, indicating a synergistic effect of the two inoculants. Co-inoculation positively impacted the index reflecting plant–microbial soil functions under both water regimes. We concluded that the interactions between the two inocula as well as between them and the resident rhizosphere microbial community were mainly negative. However, the negative interactions between R. irregularis and B. subtilis were not reflected in plant biomass. The knowledge of the plant and rhizosphere microbial responses to single and co-inoculation and their dependency on abiotic conditions is valuable for the construction of synthetic microbial communities that could be used as efficient inocula.
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Tang, Jing, Jin Nan Chen, Jin Xiang Fu, Hong Ming E, and Ming Fan. "Application and Influencing Factors of Complex Microbial Inoculants in Wastewater Treatment." Advanced Materials Research 610-613 (December 2012): 1459–62. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.1459.

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Complex microbial inoculants are highly effective compound bacteria which are widely used in the removal of pollutants, such as organic matter, nitrogen and phosphorus in the paper, printing and dyeing, petroleum and high-salt wastewater. The development of complex microbial inoculants was introduced in this paper, especially their application and influence factors in wastewater treatment. Finally we put forward current problems and development prospects of complex microbial inoculants.
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Basiru, Sulaimon, and Mohamed Hijri. "Does Commercial Inoculation Promote Arbuscular Mycorrhizal Fungi Invasion?" Microorganisms 10, no. 2 (February 9, 2022): 404. http://dx.doi.org/10.3390/microorganisms10020404.

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Interventions with commercial inoculants have the potential to reduce the environmental footprint of agriculture, but their indiscriminate deployment has raised questions on the unintended consequences of microbial invasion. In the absence of explicit empirical reports on arbuscular mycorrhizal fungi (AMF) invasion, we examine the present framework used to define AMF invasion and offer perspectives on the steps needed to avoid the negative impacts of AMF invasion. Although commercial AMF isolates are potential invaders, invasions do not always constitute negative impacts on native community diversity and functions. Instead, the fates of the invading and resident communities are determined by ecological processes such as selection, drift, dispersal, and speciation. Nevertheless, we recommend strategies that reduce overdependence on introduced inoculants, such as adoption management practices that promote the diversity and richness of indigenous AMF communities, and the development of native propagules as a supplement to commercial AMF in applicable areas. Policies and regulations that monitor inoculant value chains from production to application must be put in place to check inoculant quality and composition, as well as the transport of inoculants between geographically distant regions.
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Thanh Loi, Nguyen Thi, Vu Thi Hanh Nguyen, Pham Quynh Anh, Quach Ngoc Tung, Tran Hiep, Nguyen Thi Thu An, Chu Hoang Ha, and Phi Quyet Tien. "Effects of microbial inoculant on chemical compositions and in vitro digestibility of elephant grass-based silage." Vietnam Journal of Biotechnology 21, no. 1 (August 26, 2023): 189–96. http://dx.doi.org/10.15625/1811-4989/17406.

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Probiotic microbial inoculants have been used to improve fermentation quality of green forage feed in pig farming This study aimed to formulate and evaluate effects of microbial inoculants on quality and digestibility of elephant grass-based silage. Green forage formula was made based on economical and agro ingredients resulting in 36% of cost reduction in comparison to the basal diet. The additive containing 1% microbial mixture of Lactobacillus plantarum LCN13, Bacillus velezensis VTX9 and Saccharomyces cerevisiae MCN9 as starter cultures significantly reduced crude fiber (12.39 ± 1.01% Dry Matter (DM)) and neutral detergent fiber concentrations (28.49 ± 0.93% DM) after 120 h (p < 0.05). In contrast, remarkable increases (p < 0.05) were determined in metabolizable energy (2972 ± 18.18% DM) and crude protein (16.32±1.15% DM). These results indicated that metabolic activities by microbial inoculants contributed considerably to the nutritive value of the elephant grass-based diet. The experiment along ensiling time revealed that significant changes in nutritive compositions were observed at 120 h and 168 h (p < 0.05). In support of these results, in vitro digestibility indexes including dry matter, organic matter, and crude protein showed an increasing trend corresponding to ensiling times. Since there were no significant differences between 120 h and 168 h, fermentation time of 120 h was sufficient to obtain desired outcomes. These findings emphasized that elephant grass-based forage fermented with microbial inoculant could be a promising cost-effective and high-quality feed for pig production. Further investigations are required to evaluate safety, growth performance, and pig intestinal microbiota.
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Yadav, Aarti. "Microbial Inoculants for Sustainable Agriculture." International Journal of Current Microbiology and Applied Sciences 7, no. 05 (May 10, 2018): 800–804. http://dx.doi.org/10.20546/ijcmas.2018.705.097.

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Reddy, M. S., L. M. Funk, D. C. Covert, D. N. He, and E. A. Pedersen. "Microbial Inoculants for Sustainable Forests." Journal of Sustainable Forestry 5, no. 1-2 (April 4, 1997): 293–306. http://dx.doi.org/10.1300/j091v05n01_08.

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Khomina, Veronika, Vitalii Lapchynskyi, Zoya Pustova, Kateryna Nebaba, and Danylo Plahtiy. "Microbial inoculants as a means of improving soil and crop yields." Scientific Horizons 27, no. 10 (August 27, 2024): 79–90. http://dx.doi.org/10.48077/scihor10.2024.79.

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The article presented the results of studies of the influence of microbial inoculants Rhizobium japonicum and Azospirillum brasilense on the growth and development of soybean (Glycine max) at the initial stages of its development. The study was conducted on three experimental plots: a control plot without treatment, a plot with Rhizobium japonicum inoculum and a plot with Azospirillum brasilense inoculum. The main indicators were evaluated: plant height, number of leaves, root system development and total biomass at different stages of plant growth, as well as laboratory analysis of nitrogen content in plant tissues. The results of the study showed that the inoculants had a significant impact on all measured parameters compared to the control plot. In particular, the plants in the plot with Rhizobium japonicum showed 50% higher growth and development of the root system, which was confirmed by the formation of root nodules responsible for nitrogen fixation. Azospirillum brasilense also improved plant growth and root system development, but its effect was less pronounced compared to Rhizobium japonicum. Nitrogen content analysis showed that plants in the Rhizobium japonicum plot had 45% higher tissue nitrogen content compared to the control plot, indicating effective nitrogen fixation. In plants treated with Azospirillum brasilense, the nitrogen content was also 25% higher, but without nodule formation, the effect was less pronounced. The aim of the study was to evaluate the effectiveness of microbial inoculants in improving plant growth and development in the early stages of vegetation, to investigate their effect on root system productivity, nitrogen uptake and total plant biomass, and to determine the optimal conditions for maximising the impact of inoculants in agricultural conditions. The results emphasised the importance of using microbial inoculants to increase soybean productivity and resistance in the early stages of its development. The application of Rhizobium japonicum provided a greater increase in biomass, root system and nitrogen assimilation, which makes this inoculant more effective than Azospirillum brasilense
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Bradáčová, Klára, Maximilian Sittinger, Katharina Tietz, Benjamin Neuhäuser, Ellen Kandeler, Nils Berger, Uwe Ludewig, and Günter Neumann. "Maize Inoculation with Microbial Consortia: Contrasting Effects on Rhizosphere Activities, Nutrient Acquisition and Early Growth in Different Soils." Microorganisms 7, no. 9 (September 7, 2019): 329. http://dx.doi.org/10.3390/microorganisms7090329.

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The benefit of plant growth-promoting microorganisms (PGPMs) as plant inoculants is influenced by a wide range of environmental factors. Therefore, microbial consortia products (MCPs) based on multiple PGPM strains with complementary functions, have been proposed as superior, particularly under challenging environmental conditions and for restoration of beneficial microbial communities in disturbed soil environments. To test this hypothesis, the performance of a commercial MCP inoculant based on 22 PGPM strains was investigated in greenhouse experiments with maize on three soils with contrasting pH, organic matter content and microbial activity, under different P and N fertilization regimes. Interestingly, the MCP inoculant stimulated root and shoot growth and improved the acquisition of macronutrients only on a freshly collected field soil with high organic matter content, exclusively in combination with stabilized ammonium fertilization. This was associated with transiently increased expression of AuxIAA5 in the root tissue, a gene responsive to exogenous auxin supply, suggesting root growth promotion by microbial auxin production as a major mode of action of the MCP inoculant. High microbial activity was indicated by intense expression of soil enzyme activities involved in C, N and P cycling in the rhizosphere (cellulase, leucine peptidase, alkaline and acid phosphatases) but without MCP effects. By contrast, the MCP inoculation did not affect maize biomass production or nutrient acquisition on soils with very little Corg and low microbial activity, although moderate stimulation of rhizosphere enzymes involved in N and P cycling was recorded. There was also no indication for MCP-induced solubilization of Ca-phosphates on a calcareous sub-soil fertilized with rock-phosphate. The results demonstrate that the combination of multiple PGPM strains with complementary properties as MCP inoculants does not necessarily translate into plant benefits in challenging environments. Thus, a better understanding of the conditions determining successful MCP application is mandatory.
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Rezaeian, M., A. S. Chaudhry, and J. Honarzad. "Effects of a bacterial inoculant on chemical composition and fermentation parameters of corn silage ensiled in a laboratory silo." Proceedings of the British Society of Animal Science 2007 (April 2007): 231. http://dx.doi.org/10.1017/s1752756200021347.

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The use of lactic acid bacteria (LAB) as microbial inoculants in order to increase bacterial fermentation and decrease the pH of silage which in turn inhibit the growth of undesirable microbes and provide stable ensiling conditions has been well documented (McDonald, 1991). Although corn silage is used as major forage for dairy cattle at most commercial farms in Iran no information is available regarding the suitability of LAB for their use as inoculants in preparing corn silage. The objective of this study was therefore to determine the effects of the addition of a bacterial inoculant on chemical composition and fermentation characteristics of corn forage ensiled in 2 kg laboratory silos.
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Putri, Sindy Marieta, Iswandi Anas, Fahrizal Hazra, and Ania Citraresmini. "VIABILITAS INOKULAN DALAM BAHAN PEMBAWA GAMBUT, KOMPOS, ARANG BATOK DAN ZEOLIT YANG DISTERIL DENGAN IRADIASI SINAR GAMMA Co-60 DAN MESIN BERKAS ELEKTRON." Jurnal Ilmu Tanah dan Lingkungan 12, no. 1 (April 1, 2010): 23. http://dx.doi.org/10.29244/jitl.12.1.23-30.

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Carrier is one of the important factor to determine the quality of biofertilizer. The inoculant carriers should contain no or less microbial contaminant. The purpose of this research was to investigate viability of Azospirillum, Azotobacter and Phosphate Solubilizing Fungi inoculants in carrier material that were sterilized by Gamma Irradiation Co-60 and Electron Beam Machine (EBM). Each inoculants was injected to the sterilized peat, compost, coconut shell charcoal and zeolite. Viability of inoculants in steriled carrier was evaluated at 0, 7, 14, 42 and 70 days after preparation. The stirage was done in incubator at 25ºC. The result of viability of Azospirillum, Azotobacter and Phosphate Solubilizing Fungi inoculants on sterilized carrier material by Gamma Irradiation Co-60, EBM and autoclave tended to decline during storage 70 days at room temperature (250C). Viability of Azospirillum inoculants in zeolite sterilized by Gamma Irradiation Co-60 and autoclave gave the highest numbres of viable cells. Storage of these inoculants at 25ºC for 70 days only reduce the number of viable cells by 11.1%. Viability of Phosphate Solubilizing Fungi inoculants in coconut shell charcoal or compost sterilized by Gamma Irradiation Co-60 went down by 99.8% after 70 days. The sterilization of carriers by using autoclave or Gamma Irradiation Co-60 were better than EBM sterilization. . Keywords: Carrier, Electron Beam Machine, Gamma Irradiation Co-60, sterilization, viability
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Miteu, Goshen David, Afolabi Ayomide Emmanuel, Irenosen Addeh, Olayemi Ojeokun, Temidayo Olayinka, James Sunday Godwin, Elizabeth Oluwapelumi Folayan, and Elohozino Oghale Benneth. "The Application of Microbial Inoculants as a Green Tool towards Achieving Sustainable Agriculture." IPS Journal of Nutrition and Food Science 2, no. 2 (July 22, 2023): 52–61. http://dx.doi.org/10.54117/ijnfs.v2i2.31.

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For decades, the surging demand for agricultural produce to alleviate hunger has spurred extensive research into novel and sustainable agricultural practices. Given the detrimental environmental impact of some contemporary agricultural methods, this paper underscores the potential of microbial inoculants as environmentally friendly tools for achieving sustainable agriculture. This narrative review evaluates the use of microbial inoculants in various agricultural practices such as monocropping, crop rotation, and intercropping, contrasts their effectiveness to synthetic agricultural compounds, and highlights potential limitations and strategies for their mitigation. The review reveals that microbial inoculants, as renewable, eco-friendly, crop-supportive, and cost-effective alternatives to synthetic agricultural compounds, hold immense promise for sustainable agriculture. The study concludes with a call for greater emphasis on the use of microbial inoculants, encouraging their broader adoption by farmers to promote sustainability in agriculture.
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Selvarajoo, Rubini Devi, and Nurul 'Azyyati Sabri. "Performance of Okra and Soil Using Indigenous Microorganisms Inoculants." Malaysian Applied Biology 52, no. 4 (October 31, 2023): 19–25. http://dx.doi.org/10.55230/mabjournal.v52i4.i058.

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Microbial inoculants are beneficial microorganisms applied to plants or the soil to promote plant growth and control pest disease and weeds. Microbial inoculants isolated from local surroundings are indigenous microorganisms (IMO) inoculants. The performance of the IMO inoculants is varied depending on the sources and the local environment. Therefore, it is important to identify the right sources to enhance the efficiency of the IMO inoculants. This research aims to study the performance of okra and soil by mixing potential yeast sources for indigenous microorganisms (IMO) inoculants. Longan and mango were chosen as the sources of yeasts. The IMO inoculants were fermented for a week, and the microorganisms group was identified. Then, the IMO inoculants were applied to the okra and tested for physical and mineral content analysis. IMO inoculants with mango and longan showed a higher yeast population than the control. However, IMO inoculants with mango showed the best plant growth and harvesting time performance. The soil treated with both IMO inoculants also showed higher potassium and calcium. To conclude, plants treated with both IMO inoculants performed better than the control. Thus, IMO inoculants with longan and mango may potentially enhance the yeast community in IMO inoculants, indirectly improving okra growth and benefiting the agriculture field in the future.
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Sun, Lianhao, Yuexiang Zhou, Hui Nie, Chong Li, Xin Liu, Jie Lin, Xiongfei Zhang, and Jinchi Zhang. "Solid Microbial Fertilizers Prepared with Different Carriers Have the Potential to Enhance Plant Growth." Forests 16, no. 3 (March 19, 2025): 539. https://doi.org/10.3390/f16030539.

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Microbial inoculants are vital for promoting plant growth and facilitating the ecological restoration of degraded forested regions near abandoned mine sites. However, the direct application of liquid microbial inoculants is often challenging due to low microbial activities and poor transport efficiencies, which limit their effectiveness in complex soil environments. To tackle these challenges, this study utilized immobilized microbial technology to evaluate the effectiveness of solid microbial inoculants sourced from peat (P), biochar (BC), and spent mushroom substrates (SMSs) in enhancing the soil’s multifunctionality and promoting plant growth. Specifically, this research sought to assess the effectiveness of solid microbial inoculants derived from peat (P), biochar (B), and spent mushroom substrates (SMSs) in enhancing soil multifunctionality and promoting plant growth in nutrient-deficient soils that were affected by abandoned mine sites. We aimed to evaluate the performance of different solid microbial inoculants in improving the soil’s nutrient content and enzyme activities. A 24-week pot experiment was conducted using Medicago sativa L. in nutrient-poor soil. The results demonstrated that, in contrast to peat and biochar, SMSs effectively interacted with microbial inoculants and significantly improved the nutrient content and enzyme activities of nutrient-deficient soil. It was noted that β-1,4-glucosidase (BG), invertase, β-1,4-N-acetylglucosaminidase (NAG), urease, and soil available phosphorus increased by 204%, 405%, 118%, 198%, and 297%, respectively. The soil’s multifunctionality improved by 320% compared with the CK, and the plant biomass also increased significantly. Further, our random forest analysis indicated that the soil available phosphorus, ammonium nitrogen, total nitrogen, total carbon content, arylsulfatase, pH, total phosphorus, NAG, and BG were key environmental factors that induced changes in plant biomass. These findings highlighted the potential of SMSs as an effective carrier for immobilized microbial inoculants, which provides a sustainable approach for the restoration of forest soils surrounding abandoned mine sites, as well as a promising avenue for the valorization of agricultural waste.
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Ávila, Carla Luiza da Silva, Alexandre Rocha Valeriano, José Cardoso Pinto, Henrique César Pereira Figueiredo, Adauton Vilela de Rezende, and Rosane Freitas Schwan. "Chemical and microbiological characteristics of sugar cane silages treated with microbial inoculants." Revista Brasileira de Zootecnia 39, no. 1 (January 2010): 25–32. http://dx.doi.org/10.1590/s1516-35982010000100004.

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The aim of this research was to evaluate the effect of microbial additives containing heterofermentative or homofermentative bacteria on the chemical and microbiological characteristics of sugar cane (Saccharum spp) silages. Sugar cane was inoculated with Lactobacillus plantarum, L. paracasei, L. brevis or L. buchneri previously isolated from sugar cane silages or commercial inoculants containing L. buchneri or L. plantarum. Silages were produced in laboratory silos (10 × 60 cm PVC tubes) and evaluated 90 days after ensiling. A randomized complete design was used with eight treatments (seven inoculants and control - without inoculant) and three replications. The inoculation with bacteria affected lactic acid bacteria, yeast populations, volatile fatty acids and ethanol contents in the silages. Inoculation with different strains of the same species of bacteria result in silages with different chemical and microbiological characteristics. Two of the L. buchneri strains show the best results in relation to silage quality.
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Muck, Richard. "Recent advances in silage microbiology." Agricultural and Food Science 22, no. 1 (March 27, 2013): 3–15. http://dx.doi.org/10.23986/afsci.6718.

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Recent advances in silage microbiology are reviewed. Most new techniques in silage microbiology use the polymerase chain reaction (PCR) to make copies of a portion of the DNA in microorganisms. These techniques allow us to identify and quantify species as well as do community analysis. The PCR-based techniques are uncovering new species, both bacteria and fungi, during storage and feeding. Silage inoculants are widely available, but of greater interest has been research investigating why inoculants are so successful. Various inoculant strains have been found to produce bacteriocins and other compounds that inhibit other bacteria and fungi, improving their chances for success. In vitro ruminal fermentation research is showing that some inoculated silages affect rumen microorganisms, reducing methane in some cases and increasing microbial biomass production in others. Better understanding of silage microbiology will allow us to better manage silos and develop better inoculants to improve silage quality.
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Araújo, Fátima Natália Fontoura, Lucien Bissi da Freiria, Murilo Vargas da Silveira, Yasmim Rodrigues Vilas Boas e Silva, Fagton de Mattos Negrão, Luciano da Silva Cabral, Emerson Silva Miranda, and Flávio Henrique Bravim Caldeira. "Chemical characteristics of grass silage with the use of acidifier after opening the bag." Brazilian Journal of Animal and Environmental Research 7, no. 3 (September 16, 2024): e72853. http://dx.doi.org/10.34188/bjaerv7n3-075.

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The objective of this work was to evaluate the chemical characteristics of Miyagi grass silage (Megathyrsus maximus cv. Miyagi), with the addition of moisture sequestrants (ground corn grain - GCG, and dried distillers grain with solubles and high fiber, DDGS), and addition of inoculant (SiloSolveMC) and/or acid (Mold-Nil Liquid). The experimental design was completely randomized in a 3x3 factorial arrangement, with five replications: factor 01, the use of moisture scavengers (without additive, with the addition of GCG and/or DDGS), and factor 2, the use of bacterial inoculant (SiloSolveMC) and/or acid (Mold-Nil Liquid). For the chemical evaluation of the silage, the percentage of dry matter (DM), the insoluble fiber in neutral detergent (NDF), mineral matter contents (MM), and crude protein (CP). There were no interactions between moisture scavengers, bacterial inoculants, and/or acids. The use of moisture scavengers interfered with DM (P<0.05), observing a value with GCG and DDGS use of 36.18% and 35.46%, respectively, higher than MGS (27,68%). For NDF values using GCG and DDGS, values of 51.39% and 65.47%, respectively, lower (P<0.05) than MGS (70.72%) were observed. The use of microbial inoculant and acid did not alter (P>0.05) the OM values, with an average value of 92%. As for CP, there was no interaction (P>0.05) of moisture scavengers, microbial inoculant, and/or acid. It is recommended to include moisture-sequestering foods associated with MGS to obtain better DM, NDF, and CP values after opening the bag and the use of microbial inoculants and/or acids in the MGS did not influence these characteristics.
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G.*, Swapna, Divya M., and Brahmaprakash G.P. "Survival of Microbial consortium in granular formulations, degradation and release of microorganisms in soil." Annals of Plant Sciences 5, no. 05 (June 21, 2016): 1348. http://dx.doi.org/10.21746/aps.2016.05.004.

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The green revolution bought amazing consequences in food grain production but with insufficient concern for agriculture sustainability. Biofertilizers are gaining importance in sustaining agriculture. Various complementing combinations of microbial inoculants for management of major nutrients are necessary for agriculture sustainability. The present investigation was conducted to study the survivability of granular formulations containing Nitrogen fixing bacteria (Azotobacter chrococcum), phosphate solubilizing bacteria (Bacillus megaterium) and plant growth promoting bacteria (Pseudomonas fluorescens) in consortium prepared using different flour. Maximum survival of microbial consortium was observed in Soybean, followed by Soybean + Semolina and Rice inoculant formulations. Minimum survival of population was observed in Ragi + Semolina inoculant formulation. Wheat, wheat + semolina, soybean, soybean + semolina granular formulations have shown better degradation compared to other granular formulations both in presence and absence of tomato and finger millet (Eleusine coracana). Among different granular inoculant formulations, Maximum release was observed in soybean granular inoculant formulations in presence of tomato and Finger millet plant and in absence of plant and minimum release of microbial consortium was observed in ragi + semolina granular inoculant formulations during incubation in soil.
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Bertham, Yudhi Harini, Abimanyu Dipo Nusantara, Bambang Gonggo Murcitro, and Zainal Arifin. "PERUBAHAN KARAKTERISTIK TANAH DAN PENAMPILAN BEBERAPA VARIETAS PADI GOGO PADA KAWASAN PESISIR DENGAN PENAMBAHAN PUPUK HAYATI DAN BIOKOMPOS." Jurnal Ilmu-Ilmu Pertanian Indonesia 22, no. 2 (December 3, 2020): 79–84. http://dx.doi.org/10.31186/jipi.22.2.79-84.

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[CHANGE IN SOIL CHARATERISTICS AND PERFORMANCE OF UPLAND RICE VARIETIES IN COASTAL AREA AS AMENDED WITH BIOFERTILIZER AND BIOCOMPOST]. Dryland in the coastal area has good potential for the cultivation of upland rice to reach food self-sufficiency and the development of future agriculture. Low fertility of the land the area can be overcome by using appropriate technology such as the use of superior varieties, bio-fertilizers, and bio compost. This study aimeds to (1) determine chemical and biological properties of coastal land to improve the growth of upland rice (2) find out the growth of upland rice in the coastal area using low input technology and (3) obtain upland rice varieties with high adaptability to a coastal area environment. The experimental design used was a split-plot design with the main plot of 3 upland rice varieties, namely Inpago 10, Serantan, and Local Variety, while the subplots are were fertilizer inputs namely [double inoculant P solubilized microbial p (pf) + K solubilized microbially + N fixation microbial N], [double inoculant P solubilized microbial (fma) + K solubilized microbially + N fixation micarobia], [biocompost at a dose of 10 tons/ha], and [inorganic fertilizer recommended by BPTP ie 200 kg Urea/ha, 100 kg SP36/ha, 100 kg KCl/ha]. The results showed that the coastal area has the potential for the development of upland rice cultivation. Also, the double inoculants of biological fertilizers were able to increase plant nutrient uptake, soil biological characteristics, and the growth of upland rice as compared to controls. Specifically, the best treatment is produced by application of [double inoculant microbial solvent p (pf) + microbial solvent K + microbial N fixation] combined with upland rice Inpago variety 10.
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Mamun, Abdullah Al, Günter Neumann, Narges Moradtalab, Aneesh Ahmed, Brice Dupuis, Geoffrey Darbon, Fahim Nawaz, et al. "Microbial Consortia Versus Single-Strain Inoculants as Drought Stress Protectants in Potato Affected by the Form of N Supply." Horticulturae 10, no. 1 (January 20, 2024): 102. http://dx.doi.org/10.3390/horticulturae10010102.

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This study investigated the drought protection effects of six fungal and bacterial inoculants and ten consortia thereof on vegetative growth, nutritional status, and tuberization of potato under controlled and field conditions. It was hypothesized that microbial consortia offer improved drought protection as compared with single strains, due to complementary or synergistic effects, with differential impacts also of N fertilization management. Under NO3− fertilization, a 70% reduction in water supply over six weeks reduced shoot and tuber biomass of non-inoculated plants by 30% and 50%, respectively, and induced phosphate (P) limitation compared to the well-watered control. The P nutritional status was significantly increased above the deficiency threshold by three single-strain inoculants and eight consortia. This was associated with the presence of the arbuscular mycorrhizal fungus (AMF) inoculant Rhizophagus irregularis MUCL41833 (five cases) and stimulation of root growth (five cases). Additionally, Bacillus amyloliquefaciens FZB42 and AMF + Pseudomonas brassicacearum 3Re2-7 significantly reduced irreversible drought-induced leaf damage after recovery to well-watered conditions. However, the microbial inoculants did not mitigate drought-induced reductions in tuber biomass, neither in greenhouse nor in field experiments. By contrast, NH4+-dominated fertilization significantly increased tuber biomass under drought stress (534%), which was further increased by additional AMF inoculation (951%). This coincided with (i) improved enzymatic detoxification of drought-induced reactive oxygen species (ROS), (ii) improved osmotic adjustment in the shoot tissue (glycine betaine accumulation), (iii) increased shoot concentrations of ABA, jasmonic acid, and indole acetic acid, involved in drought stress signaling and tuberization, and (iv) reduced irreversible drought-induced leaf damage. Additional application of bacterial inoculants further improved ROS detoxification by increasing the production of antioxidants but stimulated biomass allocation towards shoot growth at the expense of tuber development. The results demonstrated that microbial consortia could increase the probability of drought protection effects influenced by the form of N supply. However, protective effects on vegetative growth do not necessarily translate into yield benefits, which can be achieved by adequate combination of inoculants and fertilizers.
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Ertekin, Ibrahim. "Effects of commercial bacterial inoculants on fermentation and nutritive quality of wheat and annual legume mixed silages." Bangladesh Journal of Botany 52, no. 3 (October 12, 2023): 775–82. http://dx.doi.org/10.3329/bjb.v52i3.68896.

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In the present study fermentation quality, microbial populations and nutritive value of wheat and some cool season annual legume (forage pea, grass pea and vetch) mixed silages (25:75, 50:50 and 75:25) using various commercial bacterial inoculants (Pioneer 1188, Pioneer 11A44 and Pioneer 11CFT) were determined. Wheat and legume species were sown in separate plots and these plants were harvested when wheat reached the dough stage about 180 days after plant emergence. The present study was conducted in a factorial (3 legume species×3 inoculants×5 mixtures) arrangement design. Results showed that effects of legume species on ADF, crude ash, pH, lactic acid bacteria, enterobacteria, yeast and mold, lactic acid, acetic acid, propionic acid, butyric acid and ammonia nitrogen were significant (P < 0.05). Crude protein, silage fermentation features and microbial populations were affected (P<0.05) by inoculants. Effects of mixture ratios on dry matter, chemical compositions, relative feed value, fermentation parameters of silages were significant (P < 0.05). It was determined that ensiling the 50 % wheat with 50% grass pea with the use of inoculant 1188 as an additive could give a good result both in terms of nutritive value and fermentation quality. Bangladesh J. Bot. 52(3): 775-782, 2023 (September)
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Santos, Lidiane Figueiredo dos, Marliane De Cássia Soares Silva, Rogério De Paula Lana, Nayron Vilela Diogo, Maria Catarina Megumi Kasuya, and Karina Guimarães Ribeiro. "Effective microorganisms: Microbial diversity and its effect on the growth of palisade grass." Tropical Grasslands-Forrajes Tropicales 8, no. 3 (September 30, 2020): 177–86. http://dx.doi.org/10.17138/tgft(8)177-186.

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Effective microorganisms (EM) are inoculants used by farmers on various crops, and the actual efficiency of EM and their composition have been widely discussed. The objective of this study was to analyze the profile of the microbial community in soils after applying 3 EM inoculants from different origins with and without manure and to determine the impacts on growth and chemical composition of Urochloa brizantha (palisade grass). We showed, by PCR-DGGE technique, that the community structure of the fungi and bacteria in soil differed with EMs from different sources and that adding manure to the soil also significantly altered the bacterial and fungal profile. We also found that adding manure to soil resulted in a pronounced increase in both dry matter yield and crude protein concentration in palisade grass, while benefits of applying EM were largely restricted to a farmer-produced inoculant, where CP% was increased and NDF% was reduced when applied along with manure.
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Bhardwaj*, Vibha. "Microbial Inoculant: A Potential and Sustainable way for Improvement and Production of Crops for Agricultural Sector Development." Journal of Biomedical Research & Environmental Sciences 6, no. 2 (February 2025): 204–11. https://doi.org/10.37871/jbres2073.

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Introduction: Increasing demand for food due to rapidly increase in global population, it is necessary to meet the food requirements without degrading the environment. In cultivated zones, around highly populated areas, there is excessive use of fertilizers. There is a requirement to opt for natural or biological fertilizers to substitute the chemical fertilizers, due to the increasing demand for agriculture sustainability. Microbial Inoculants could be effective for crop production improvement without negative effects on the environment. Objective: The present study focuses on the efficacy and utilization of Bacillus subtilis (ATCC 6633) and Pseudomonas sp. (ATCC 27853) as microbial inoculant to analyse the growth rate and of mustard (Brassica nigra) and carom seeds (Trachyspermum ammi) and compare with untreated seeds. Bacterial culture in the form of seed treatment and foliar spray were used as microbial Inoculant for the growth of plant. Results and Discussion: The major seed treatment effect was observed with Bacillus subtilis that showed antifungal activity against phytopathogens. Initially, faster germination percentage was found with the carom seeds with seed treatment showed the best results, with 90% and 82% in 3 days compared to the untreated seeds and mustard seeds. Mustard Seeds treated with Bacillus subtilis showed faster plant growth rate after sowing in terms of increase in shoot length 11.6cm, 13.2 cm and root length 2.3cm, respectively, after 20 days. Spraying treatment resulted in shoot length 6.2 cm and root length 1.4cm, respectively, higher than compared to the control. Conclusion: Bacillus subtilis ATCC 6633 and Pseudomonas sp. (ATCC 27853) microbial inoculum can be used as Microbial Inoculants, and they could act as an environmental-friendly and economical alternative to synthetic liquid fertilizer for promoting sustainable agriculture.
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Naamala, Judith, and Donald L. Smith. "Relevance of Plant Growth Promoting Microorganisms and Their Derived Compounds, in the Face of Climate Change." Agronomy 10, no. 8 (August 12, 2020): 1179. http://dx.doi.org/10.3390/agronomy10081179.

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Climate change has already affected food security in many parts of the world, and this situation will worsen if nothing is done to combat it. Unfortunately, agriculture is a meaningful driver of climate change, through greenhouse gas emissions from nitrogen-based fertilizer, methane from animals and animal manure, as well as deforestation to obtain more land for agriculture. Therefore, the global agricultural sector should minimize greenhouse gas emissions in order to slow climate change. The objective of this review is to point out the various ways plant growth promoting microorganisms (PGPM) can be used to enhance crop production amidst climate change challenges, and effects of climate change on more conventional challenges, such as: weeds, pests, pathogens, salinity, drought, etc. Current knowledge regarding microbial inoculant technology is discussed. Pros and cons of single inoculants, microbial consortia and microbial compounds are discussed. A range of microbes and microbe derived compounds that have been reported to enhance plant growth amidst a range of biotic and abiotic stresses, and microbe-based products that are already on the market as agroinputs, are a focus. This review will provide the reader with a clearer understanding of current trends in microbial inoculants and how they can be used to enhance crop production amidst climate change challenges.
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