Auswahl der wissenschaftlichen Literatur zum Thema „Mesoscale organisation“

Significant research has investigated synchronisation in brain networks, but the bulk of this work has explored the contribution of brain networks at the macroscale. Here we explore the effects of changing network topology on functional dynamics in spatially constrained random networks representing mesoscale neocortex. We use the Kuramoto model to simulate network dynamics and explore synchronisation and critical dynamics of the system as a function of topology in randomly generated networks with a distance-related wiring probability and no preferential attachment term. We show networks which predominantly make short-distance connections smooth out the critical coupling point and show much greater metastability, resulting in a wider range of coupling strengths demonstrating critical dynamics and metastability. We show the emergence of cluster synchronisation in these geometrically-constrained networks with functional organisation occurring along structural connections that minimise the participation coefficient of the cluster. We show that these cohorts of internally synchronised nodes also behave en masse as weakly coupled nodes and show intra-cluster desynchronisation and resynchronisation events related to inter-cluster interaction. While cluster synchronisation appears crucial to healthy brain function, it may also be pathological if it leads to unbreakable local synchronisation which may happen at extreme topologies, with implications for epilepsy research, wider brain function and other domains such as social networks.

Abstract. This paper presents first results on the investigation of the synoptic conditions that led to the rapid development of a low-pressure system over the Aegean Sea. Indeed, during the period 21–22 January 2004, a very deep cyclone was observed over the Aegean Sea with a minimum central pressure of ~972 hPa, a value which is among the lowest observed over the entire Mediterranean Sea during the last 40 years. The rapid development was associated with a two-trough system that, under the influence of a very intense upper-level jet, was merged in one and then acquired a negative tilting. Additional information on the mesoscale organisation of the system is given, based on lightning data and space borne microwave and infrared observations.

Abstract. The general objective of the international MEDiterranean EXperiment (MEDEX) was the better understanding and forecasting of cyclones that produce high impact weather in the Mediterranean. This paper reviews the motivation and foundation of MEDEX, the gestation, history and organisation of the project, as well as the main products and scientific achievements obtained from it. MEDEX obtained the approval of World Meteorological Organisation (WMO) and can be considered as framed within other WMO actions, such as the ALPine EXperiment (ALPEX), the Mediterranean Cyclones Study Project (MCP) and, to a certain extent, THe Observing System Research and Predictability EXperiment (THORPEX) and the HYdrological cycle in Mediterranean EXperiment (HyMeX). Through two phases (2000–2005 and 2006–2010), MEDEX has produced a specific database, with information about cyclones and severe or high impact weather events, several main reports and a specific data targeting system field campaign (DTS-MEDEX-2009). The scientific achievements are significant in fields like climatology, dynamical understanding of the physical processes and social impact of cyclones, as well as in aspects related to the location of sensitive zones for individual cases, the climatology of sensitivity zones and the improvement of the forecasts through innovative methods like mesoscale ensemble prediction systems.

Cells typically respond to a variety of geometrical cues in their environment, ranging from nanoscale surface topography to mesoscale surface curvature. The ability to control cellular organisation and fate by engineering the shape of the extracellular milieu offers exciting opportunities within tissue engineering. Despite great progress, however, many questions regarding geometry-driven tissue growth remain unanswered.Here, we combine mathematical surface design, high-resolution microfabrication, in vitro cell culture, and image-based characterization to study spatiotemporal cell patterning and bone tissue formation in geometrically complex environments. Using concepts from differential geometry, we rationally designed a library of complex mesostructured substrates (101-103 µm). These substrates were accurately fabricated using a combination of two-photon polymerisation and replica moulding, followed by surface functionalisation. Subsequently, different cell types (preosteoblasts, fibroblasts, mesenchymal stromal cells) were cultured on the substrates for varying times and under varying osteogenic conditions. Using imaging-based methods, such as fluorescent confocal microscopy and second harmonic generation imaging, as well as quantitative image processing, we were able to study early-stage spatiotemporal cell patterning and late-stage extracellular matrix organisation. Our results demonstrate clear geometry-dependent cell patterning, with cells generally avoiding convex regions in favour of concave domains. Moreover, the formation of multicellular bridges and collective curvature-dependent cell orientation could be observed. At longer time points, we found clear and robust geometry-driven orientation of the collagenous extracellular matrix, which became apparent with second harmonic generation imaging after ∼2 weeks of culture.Our results highlight a key role for geometry as a cue to guide spatiotemporal cell and tissue organisation, which is relevant for scaffold design in tissue engineering applications. Our ongoing work aims at understanding the underlying principles of geometry-driven tissue growth, with a focus on the interactions between substrate geometry and mechanical forces.

Abstract. The general objective of the international MEDiterranean EXperiment (MEDEX) was the better understanding and forecasting of cyclones that produce high impact weather in the Mediterranean. This paper reviews the motivation and foundation of MEDEX, the gestation, history and organisation of the project, as well as the main products and scientific achievements obtained from it. MEDEX obtained the approval of WMO and can be considered as framed within other WMO actions, such as ALPEX, MCP and, to certain extent, THORPEX and HyMeX. Through two phases (2000–2005 and 2006–2010) MEDEX has produced a specific database, with information about cyclones and high impact weather events, several main reports and a specific field campaign (DTS-MEDEX-2009). The scientific achievements are significant in fields like climatology, dynamical understanding of the physical processes and social impact of cyclones, as well as on aspects related to the location of sensitive zones for individual cases, climatology of sensitivity zones and the improvement of the forecasts through innovative methods like mesoscale ensemble prediction systems.

This paper presents the tracking and short-term forecasting of mesoscale convective cloud clusters (CCs) that occurred over southeast Brazil and the adjacent Atlantic Ocean during 2009–17. These events produce intense rainfall and severe storms that impact agriculture, defence, hydroelectricity and offshore oil production. To identify, track and forecast CCs, the Geostationary Operational Environmental Satellite infrared imagery and the Forecasting and Tracking the Evolution of Cloud Clusters method are used. The forecast performance is investigated by applying statistical analyses between the observed and forecasted CCs’ physical properties. A total of 7139 mesoscale convective CCs were identified, tracked and selected for the short-term forecasting at their maturation phases. The CC tracking showed a high frequency of CCs over the Atlantic Ocean and mainly over continental and coastal southeast Brazil during the wet season. This indicates an important role played by the cold fronts and convective diurnal forcing on the organisation of convective cloudiness over that region. The majority of the CCs reached their maturation phases within the first 2h of life cycle, which occurred mostly between the late afternoon and evening. The CCs had short lifetimes and were predominantly in meso-β scales, followed by meso-α convective CCs. The CCs showed cloud-top temperatures typical of clouds with strong vertical development and potential to produce rainfall. The short-term forecasting of CCs at their maturation phases revealed different behaviours of the statistical indices with forecast range. For the 30–60-min timeframe, the forecasts performed relatively well. For longer forecast lead times (90–120min), the forecasts overestimated the occurrences, intensities and growth of the CCs and forecasted the CCs to be further north and east of their actual observed locations. Overall, our results may contribute to improving the forecast quality of these intense weather events.

The thesis is presented in alternative format, meaning that the results of the thesis take the form of three journal articles, each telling a distinct story within the subject matter, but collectively highlighting the sensitivity of bands to frictional and diabatic processes. Paper 1 is an idealised-modelling study with the Weather Research and Forecasting (WRF) model, in which moist baroclinic waves are simulated from an initial zonally uniform mid-latitude jet on an f-plane at 20-km grid spacing, and the sensitivity of the resulting precipitation bands is explored. Paper 2 employs further WRF idealised-baroclinic-wave simulations and takes a simulation from Paper 1, after the cold front has formed, as the initial condition. A nested domain at 4-km grid spacing is inserted when this simulation is re-initialised to invesigate the sensitivity of finer-scale precipitation cores along the surface cold front. In both Papers 1 and 2, friction and latent-heat release enhance multiple banding at the two distinct horizontal scales, while surface fluxes hinder multiple banding. Paper 3 studies post-frontal snowbands over the English Channel and Irish Sea during extreme cold-air outbreaks in the winters of 2009-10 and 2010-11, via a climatology of precipitation-radar, sounding, and SST data, and real-data WRF sensitivity simulations of one such band over the English Channel. The observational and modelling results show that strong winds and large differential heat fluxes between land and sea were necessary to generate banded precipitation. Coastal orography and the land-sea frictional contrast aided the morphology of bands, but banded precipitation did still form in the absence of these influences in the sensitivity simulations. These three studies and the thesis as a whole highlight the role of frictional and diabatic processes in modifying various types of precipitation bands within baroclinic waves, and in generating bands that would otherwise not exist.

La chimie covalente dynamique est un outil efficace pour former des (co)polymères à blocs d’architecture complexe, difficilement atteignables par voies classiques, mais aussi développer des matériaux qui répondent aux enjeux écologiques et économiques (recyclables, réparables, bon marché). Dans ce travail, nous avons implémenté la liaison imine dans des copolymères en peigne et des co-réseaux de PDMS et PEG, par une voie "grafting to" d’oligomères PEG fonctionnalisés benzaldéhyde et d’un polymère PDMS à fonctions amines pendantes. Dans une première partie, nous avons étudié les propriétés des co-réseaux PDMS-r-PEG en fonction du taux de réticulation imine. Les matériaux sont souples, peu ou pas collants et transparents. L’analyse thermo-mécanique montre qu’ils sont micro-séparés mais sans ordre à longue distance (AFM, SAXS). Ils sont reprocessables à chaud et recyclables par voie chimique (en milieu acide ou en présence d’amine). Ces propriétés s’appuient sur la dynamique associative d’échange et la réversibilité de la liaison imine.Dans une seconde partie, nous avons étudié l’auto-assemblage en solution aqueuse des copolymères en peigne PDMS-g-PEG en fonction du taux de greffage de chaines PEG. Pour un taux de greffage suffisant, les copolymères forment des suspensions colloïdales stables sur plusieurs mois mais dont la forme/taille évolue avec l’hydrolyse des liens imines. Leur capacité à encapsuler et relarguer un composé hydrophobe, en phase simple et en émulsion, a également été démontrée<br>Dynamic covalent chemistry is an effective tool to synthetize complex architectural block (co)polymers which are difficult to achieve by conventional techniques. It permits also to develop materials that meet ecological and economic challenges (recycling, sustainability, cheap raw materials and products). In this work, we implemented imine bonds in amphiphilic comb-like copolymers and co-networks starting from PDMS with pendant amines and benzaldehyde-functionalized PEG oligomers. In a first part, we studied the properties of PDMS-r-PEG co-networks as a function of the imine crosslinking rate. The materials are flexible, little or no sticky and transparent. Thermo-mechanical analysis shows that they are micro-separated but with no long-distance order (AFM, SAXS). They can be thermally reprocessed and chemically recycled (acidic medium or in the presence of an amine), thanks to the reversible and exchangeable imine crosslinking points. In a second part, we studied the self-assembly in aqueous solution of PDMS-g-PEG comb-like copolymers with the degree of grafting of PEG chains. For a sufficient degree of grafting, the copolymers form colloidal suspensions which are stable over several months even though their shape / size change with the hydrolysis of the imine bonds. Their ability to encapsulate and release a hydrophobic compound, in single phase and in emulsion, has also been demonstrated