Thematische Bibliographien / Lyoprotectants

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Auswahl der wissenschaftlichen Literatur zum Thema „Lyoprotectants“

Autor: Grafiati

Veröffentlicht am 28. Juni 2021

Zuletzt aktualisiert am 10. Februar 2022

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Zeitschriftenartikel zum Thema "Lyoprotectants"

Li, Li, Xu Yan Zong, Li Xiong, Jing Zhang und Kai Chen. „Optimization of Protection Condition for Composite Freeze-Dried Starter of Paocai“. Advanced Materials Research 798-799 (September 2013): 1087–90. http://dx.doi.org/10.4028/www.scientific.net/amr.798-799.1087.

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To obtain the better formula of the biomaterials lyoprotectant for composite starter of Paocai, five lyoprotectants were studied with math method those are orthogonal design, Quadratic polynomial stepwise regression and etc. The results showed, 14.7% of hydrolyzate of skim milk, 1.1% of amount of glucose, 3.4% of amount of glycerol, 5.0% of amount of sodium glutamate, 1.2% of amount of Vc were added to the culture of Lactobacillus. After lyophilized, colonies of freeze-dried starters can reach 3.25×1010 cfu/mL. Response surfaces are drawn according to the mathematical model among factors, and it was discussed the impact of lyoprotectants.

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Woo, Joshua, und Jeoung Soo Lee. „Effects of lyoprotectants on long-term stability and transfection efficacy of lyophilized poly(lactide-co-glycolide)-graft-polyethylenimine/plasmid DNA polyplexes“. Nanomedicine 16, Nr. 15 (Juni 2021): 1269–80. http://dx.doi.org/10.2217/nnm-2021-0065.

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Aim: We investigated the effect of lyoprotectants on the long-term stability and transfection efficiency of lyophilized (Lyo.) polyplexes prepared from poly(lactide-co-glycolide)-graft-polyethylenimine (PgP) and plasmid DNA encoding green fluorescent protein (pGFP). Materials & methods: Lyo. PgP/pGFP polyplexes prepared with/without lyoprotectants were stored at -20°C over 6 months. Polyplex stability was analyzed by gel electrophoresis and heparin competition assay. Transfection efficiency and cytotoxicity were evaluated in rat glioma (C6) cells in medium containing 10% serum. Results: Lyo. PgP/pGFP polyplexes prepared with 5% sucrose as a lyoprotectant remained stable up to 6 months and retained transfection efficiency up to 4 months. Conclusion: Lyo. PgP-based polyplexes retain bioactivity during extended storage, potentially enabling transport to remote regions and less stable settings, increasing access to life-changing gene therapy.

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Songyang, Lin, Kang Qiaozhen, Pan Dan, Liu Xin, Lu Laizheng, Hao Limin und Lu Jike. „Identification of Lyoprotectants Released from Gradient-freezing Pretreated Entocyte in Lactococcus Lactis Subsp. Lactis IL1403“. Current Topics in Nutraceutical Research 17, Nr. 2 (29.07.2017): 122–34. http://dx.doi.org/10.37290/ctnr2641-452x.17:122-134.

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Performance of Lactococcus lactis as a starter culture in food production largely depends on the use of lyoprotectants during lyophilization. Gradient-freezing of bacterial cultures was conducted at 4°C, −20°C, and −80°C by storing the samples at each temperature for 2 h, successively. The entocytes extracted from the frozen cells were used as a lyoprotectant in the follow-up freeze-drying process of Lactococcus lactis subsp. lactis IL1403. The cell survival rate of gradient-freezing group increased 6.4-fold by bacterial plate count method. Furthermore, a proteomics and bioinformatics method was applied to elucidate the protein changes of Lactococcus lactis subsp. lactis IL1403 in response to gradient-freezing by gel-free proteomics using tandem mass tags (TMTs). The results showed that 121 stress-related proteins were significantly influenced by gradient-freezing. These proteins were involved in several metabolism pathways including ribosome metabolism, amino acid metabolism, quorum sensing, phosphotransferase system (PTS), pentose phosphate pathway, microbial metabolism in diverse environments, and nitrogen metabolism, etc. Some of these proteins especially the up-regulated proteins are potential lyoprotectants in vitro and they still need to be further investigated.

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MacFarlane, D. R., J. Pringle und G. Annat. „Reversible self-polymerizing high Tg lyoprotectants“. Cryobiology 45, Nr. 2 (Oktober 2002): 188–92. http://dx.doi.org/10.1016/s0011-2240(02)00127-x.

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Qi, Kangru, He Chen, Hongchang Wan, Man Hu und Yuxi Wu. „Response Surface Optimization of Lyoprotectant from Amino Acids and Salts for Bifidobacterium Bifidum During Vacuum Freeze-Drying“. Acta Universitatis Cibiniensis. Series E: Food Technology 21, Nr. 2 (01.12.2017): 3–10. http://dx.doi.org/10.1515/aucft-2017-0009.

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Abstract High quality probiotic powder can lay the foundation for the commercial production of functional dairy products. The freeze-drying method was used for the preservation of microorganisms, having a deleterious effect on the microorganisms viability. In order to reduce the damage to probiotics and to improve the survival rate of probiotics during freeze-drying, the Response Surface Methodology (RSM) was adopted in this research to optimize lyoprotectant composed of amino acids (glycine, arginine) and salts (NaHCO3 and ascorbic acid). Probiotic used was Bifidobacterium bifidum BB01. The regression model (p<0.05) was obtained by Box–Behnken experiment design, indicating this model can evaluate the freeze-drying survival rate of B. bifidum BB01 under different lyoprotectants. The results indicated these concentrations as optimal (in W/V): glycine 4.5%, arginine 5.5%, NaHCO3 0.8% and ascorbic acid 2.3%, respectively. Under these optimal conditions, the survival rate of lyophilized powder of B. bifidum BB01 was significantly increased by 80.9% compared to the control group (6.9±0.62%), the results were agreement with the model prediction value (88.7%).

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Kelly, Jessica M., Elizabeth E. Pearce, Douglas R. Martin und Mark E. Byrne. „Lyoprotectants modify and stabilize self-assembly of polymersomes“. Polymer 87 (März 2016): 316–22. http://dx.doi.org/10.1016/j.polymer.2016.02.007.

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Hariyadi, Dewi Melani, Tutiek Purwanti und Destia Wardani. „Stability of Freeze-Dried Ovalbumin-Alginate Microspheres with Different Lyoprotectants“. Research Journal of Pharmacy and Technology 9, Nr. 1 (2016): 20. http://dx.doi.org/10.5958/0974-360x.2016.00005.6.

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Yun, Gyiae, Iqra Haleem, Hyeongmin Kim, Saemi Yoon, Ki‐Hwan Park und Jaehwi Lee. „Redispersible Freeze‐dried Quercetin‐loaded Liposomal Formulations Stabilized with Lyoprotectants“. Bulletin of the Korean Chemical Society 40, Nr. 6 (Juni 2019): 594–97. http://dx.doi.org/10.1002/bkcs.11717.

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Debulis, Katherine, und Alexander M. Klibanov. „Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants“. Biotechnology and Bioengineering 41, Nr. 5 (05.03.1993): 566–71. http://dx.doi.org/10.1002/bit.260410509.

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D'Andrea, G., M. L. Salucci und L. Avigliano. „Effect of lyoprotectants on ascorbate oxidase activity after freeze-drying and storage“. Process Biochemistry 31, Nr. 2 (Januar 1996): 173–78. http://dx.doi.org/10.1016/0032-9592(95)00045-3.

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Dissertationen zum Thema "Lyoprotectants"

Wilding, Kristen Michelle. „Engineering Cell-Free Biosystems for On-Site Production and Rapid Design of Next-Generation Therapeutics“. BYU ScholarsArchive, 2018. https://scholarsarchive.byu.edu/etd/7713.

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While protein therapeutics are indispensable in the treatment of a variety of diseases, including cancer, rheumatoid arthritis, and diabetes, key limitations including short half-lives, high immunogenicity, protein instability, and centralized production complicate long-term use and on-demand production. Site-specific polymer conjugation provides a method for mitigating these challenges while minimizing negative impacts on protein activity. However, the location-dependent effects of polymer conjugation are not well understood. Cell-free protein synthesis provides direct access to the synthesis environment and rapid synthesis times, enabling rapid evaluation of multiple conjugation sites on a target protein. Here, work is presented towards developing cell-free protein synthesis as a platform for both design and on-demand production of next generation polymer-protein therapeutics, including (1) eliminating endotoxin contamination in cell-free reagents for simplified therapeutic preparation, (2) improving shelf-stability of cell-free reagents via lyophilization for on-demand production, (3) coupling coarse-grain simulation with high-throughput cell-free protein synthesis to enable rapid identification of optimal polymer conjugation sites, and (4) optimizing cell-free protein synthesis for production of therapeutic proteins

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Švehlíková, Ingrid. „Lyofilizace polymerních nanomateriálů“. Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-445933.

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Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: PharmDr. Ondřej Holas, PhD. Student: Ingrid Švehlíková Title od thesis: Lyophilization of polymeric nanoparticles Lyophilization is a widely used drying method with extensive application possibilities in the drug preparation. Its importance in pharmacy is growing because it is one of the important methods of stabilizing active substances, especially proteins. A detailed understanding of the properties of the drug and the physicochemical phenomena of the individual phases of lyophilization is a basic prerequisite for the preparation of a safe, effective and stable drug. Biomedical nanoparticles as drug carriers are the type of the dosage forms in which lyophilization is also used. A series of lyophilization experiments were performed using trehalose, mannitol, dextran and xylitol as cryo and lyoprotectant. The parameters assessed were particle size, PDI, appearance and lyophilisate reconstitution. The properties of nanoparticles prepared by nanoprecipitation from PLGA polymer were evaluated. Furthermore, experiments were performed to validate the deep-freezing method as an alternative method to lyophilization for long-term storage of nanoparticles. The nanoparticles were stored in...

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Tseng, Tzu-Yun, und 曾子芸. „Studies of fermentation conditions and lyoprotectant for Lactobacillus paracasei subsp. paracasei NTU 101“. Thesis, 2018. http://ndltd.ncl.edu.tw/handle/bfke4j.

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碩士
國立臺東大學
生命科學系碩士班
106
Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) is a health food material with a variety of effects. However, no studies have been made on fermentation and adding freeze-drying lyoprotectants effect of subsequent storage stability. In this study In this study, the effect of oxygen level or different pH value on the growth kinetics of NTU 101 was investigated in a 2.0 L fermentor. The results showed that culture the NTU 101 with N2 gas using an orifice sparger had the highest cell density ((2.36 ± 0.21)x109 CFU/mL) and specific productivity ((1.97 ± 0.18)x108 CFU/mL/h). However, the highest specific growth rate (0.61 h-1, p < 0.05) was achieved under the control of pH 6.0. The fermentation time was shortened to 9 hours (0.5625 times decrease), 10% (w/v) soybean milk powder as an alternative medium after under the same conditions culturing NTU 101, the unit cost increased 1.53 times comparing to pH 6.0 group, which effectively reduced the production cost. In the other study, the long-term stored in room temperature after 12 months, the result showed that count of NTU 101 freeze-drying powder down to 106 CFU/g, that was not conducive to product sales. Therefore, the effect of adding lyoprotectants on the storage stability of freeze-drying powder was further investigated. The results showed that viable cells numbers of 10% skim milk, 10% trehalose and 10% lactose was (9.34 ± 4.43)x106, (1.57 ± 1.16)x106 and (2.18 ± 0.31)x107 CFU/g DCW, compared with control (no protective agent) increased 31.04, 6.16 and 65.52 times survival rate, respectively. It is conducive to apply on the subsequent manufacturing process. Using RT-qPCR analyzed the effect of oxidative stress, pH value, cold, heat and osmotic stress for NTU 101 stress gene expression. The results showed that culture with N2 gas decreased the expression of DnaJ, DnaK, sHsp and GroES, then controlling the optimal pH would further reduce the expression of DnaJ, DnaK and GroES, indicating the growth restriction of NTU 101 is related to the expression of these three genes. Under aerobic, higher or lower pH conditions expressed Csp, DnaJ, DnaK, sHsp, and GroES are all involved in the protein folding repair mechanism, protein misfolding caused by environmental stress may be the main limiting factor for the growth of NTU101 in this study. In future, it could use of these gene expression as an indicator of NTU 101 physiological status and further explores the relevance of stress treatment to survival.

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Vuová, Ngoc Lien. „Formulace lyofilizovaných tablet pro bukální aplikaci vakcín“. Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-449105.

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Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Mentor: Doc. PharmDr. Zdeňka Šklubalová, Ph.D. Student: Ngoc Lien Vuová Title of Thesis: Formulation of freeze dried tablets for buccal application of vaccines Mucosal vaccines represent an attractive way of vaccination with an advantage of inducing both systemic and local immunity. The aim of this diploma thesis was designing a composition of freeze-dried tablets for buccal administration of a model Bordetella pertusis vaccine. The easy removal from blister, firmness and aesthetic appearance as well as the appropriate taste and mucoadhesivity were the required product quality parameters. The excipients were characterised by differential scanning calorimetry, osmolality and pH measurement; the mechanical properties and disintegration time of freeze-dried tablets were evaluated. Among the variety of studied excipients and their combinations, dextran 40 as the main component of the preparation provided the best results. For the further modification of properties, the addition of fish gelatin, iota carrageenan and macrogol 300 is suitable. Freeze-dried tablets containing trehalose, mannitol and povidon 25 did not achieve the desirable quality parameters. Freeze- dried tablets containing iota carrageenan...

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Macáková, Eliška. „Formulace lyofilizovaných tablet pro orální aplikaci peptidů“. Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-411612.

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Charles University, Faculty of Pharmacy in Hradec Králové Department of: Pharmaceutical Technology Consultant: Doc. PharmDr. Zdeňka Šklubalová, Ph.D. Student: Eliška Macáková Title of Thesis: Formulation of freeze dried tablets for oromucosal administration of peptides Freeze-drying is one out of the important methods for stabilization of active substances, particularly peptides, in pharmacy. The formulation of freeze-dried buccal/sublingual tablets for administration of peptides into the oral cavity is the main target of this thesis. The aim is to propose the combination of appropriate excipients and their concentration to achieve the suitable organoleptic properties and disintegration time of the product cake. The measurement of pH, osmolality, the thermal properties of substances, as well as the evaluation of mechanical quality of tablets and their disintegration were used. In conclusion, the combination of excipients for the composition of matrix for the futher development.

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Konferenzberichte zum Thema "Lyoprotectants"

Azoddein, Abdul Aziz Mohd, Yana Nuratri, Faten Ahada Mohd Azli und Ahmad Bazli Bustary. „Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant“. In ADVANCED MATERIALS FOR SUSTAINABILITY AND GROWTH: Proceedings of the 3rd Advanced Materials Conference 2016 (3rd AMC 2016). Author(s), 2017. http://dx.doi.org/10.1063/1.5010534.

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