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1
Li, Shen, Matthias Zenkel, Friedrich E. Kruse, Andreas Gießl und Ursula Schlötzer-Schrehardt. „Identification, Isolation, and Characterization of Melanocyte Precursor Cells in the Human Limbal Stroma“. International Journal of Molecular Sciences 23, Nr. 7 (29.03.2022): 3756. http://dx.doi.org/10.3390/ijms23073756.
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Given their vital role in the homeostasis of the limbal stem cell niche, limbal melanocytes have emerged as promising candidates for tissue engineering applications. This study aimed to isolate and characterize a population of melanocyte precursors in the limbal stroma, compared with melanocytes originating from the limbal epithelium, using magnetic-activated cell sorting (MACS) with positive (CD117/c-Kit microbeads) or negative (CD326/EpCAM or anti-fibroblast microbeads) selection approaches. Both approaches enabled fast and easy isolation and cultivation of pure limbal epithelial and stromal melanocyte populations, which differed in phenotype and gene expression, but exhibited similar functional properties regarding proliferative potential, pigmentation, and support of clonal growth of limbal epithelial stem/progenitor cells (LEPCs). In both melanocyte populations, limbus-specific matrix (laminin 511-E8) and soluble factors (LEPC-derived conditioned medium) stimulated melanocyte adhesion, dendrite formation, melanogenesis, and expression of genes involved in UV protection and immune regulation. The findings provided not only a novel protocol for the enrichment of pure melanocyte populations from limbal tissue applying easy-to-use MACS technology, but also identified a population of stromal melanocyte precursors, which may serve as a reservoir for the replacement of damaged epithelial melanocytes and an alternative resource for tissue engineering applications.
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Imran, Maya, Rosman Ilato, Fitri Hadi Yulia, Meyko Panigoro, Ardiansyah Ardianyah und Sudirman Sudirman. „Pengaruh Pengajaran Remedial Terhadap Pencapaian Kriteria Ketuntasan Minimal Pada Mata Pelajaran IPS Terpadu“. Journal of Economic and Business Education 1, Nr. 3 (07.11.2023): 111–22. http://dx.doi.org/10.37479/jebe.v1i3.20332.
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This study aims to determine the influence of remedial teaching on the achievement of the minimum completeness criteria in the integrated sosial subject in grade VII of SMP Negeri 2 Limboto. The research used an expereimental method with One Group Pretest-Postest Design. The total populations were 86 students, and the samples were 46 students determined through probality sampling. This research was quantitative reswarch with a descriptive analysis teachnique. The results showed that the infuence of remedial teaching on the achievement of the minimun completeness criteria in the integrated social science subject in grade VII of SMP Negeri 2 Limbboto was that there was a difference in the average pretest and posttest scores. The average pretest score was 49,13, while the posttest was 80,09. The percentage obtained before and after remedial teaching was 63% in other words remedial teaching influenced the achiement of the minimum completeness criteria in the integrated social science subject in grade VII of SMP Negeri 2 Limboto.
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Ibrahim, Ahmad Yusuf, Supramana und Giyanto. „Populasi Nematoda Tanah pada Perlakuan Limbah Tanaman Brassicaceae“. Jurnal Fitopatologi Indonesia 19, Nr. 1 (06.03.2023): 19–29. http://dx.doi.org/10.14692/jfi.19.1.19-29.
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Population of Soil Nematodes in The Treatment of Brassicaceae Plant Waste Brassicaceae plants are known to contain glucosinolates which can be hydrolyzed into biofumigant compounds. This research was conducted to examine the effect of biofumigant derived from Brassicaceae plants waste on soil nematode population dynamics. Greenhouse experiment was carried out on 4 types of Brassicaceae plants waste, consisting of leaf waste of radish (Raphanus sativus), broccoli (Brassica oleracea var. italica), cabbage (Brassica oleracea var. capitata), and whole parts kamanilan weed (Roripa indica) in polybags containing 5 L of nematode infested soil. Plant waste was chopped ± 1 cm in size, then as much as 117 g per polybag was mixed in nematode infested soil, doused with water until wet and tightly closed for the biofumigation process for 14 days. At the end of biofumigation step, the polybags were opened for 3-5 days, then 7 day - old cucumber seedlings of var. Roberto 92 were planted and the plants were maintained in greenhouse for up to 8 weeks. Observation of the type and population of soil nematodes was carried out three times, i.e. before treatment, after treatment, and 8 weeks after planting. This experiment was designed in a completely randomized design with 6 treatments and 5 replications. The results of the study showed that the population of free-living nematodes (bacteriovorous nematodes and fungivorous nematodes) was increased in all treatment of Brassicaceae plants waste. On the other hand, there was a significant decrease in the phytonematodes population in the treatment of broccoli leaf waste. All Brassicaceae wastes was able to suppress population of Helicotylenchus sp., Rotylenchulus sp., and Xiphinema sp., significantly. Leaf waste of broccoli leaves showed the highest inhibition value against all phytonematodes genera with inhibition reached 100%.
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Ibrahim, Mustamin, Ilyas H. Husain und Tutin Pratiwi Langaru. „Biodiversity of Migrant Birds in the Limboto Lake Region, Gorontalo Province“. Jurnal Biologi Tropis 23, Nr. 3 (25.06.2023): 282–89. http://dx.doi.org/10.29303/jbt.v23i3.5246.
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The bird population in Indonesia is rich in diversity, this is based on the number of bird populations and environmental conditions that are still maintained so as to allow the birds to live. This study aims to describe the types of migratory birds in Lake Limboto. The method used in this study is the count point method and with 4 observation points for 7 days on different days. The starting point of the observations is carried out alternately for each station so that there are variations in the observation time at each station. Overall, the classification of migratory birds in the Limboto Lake area is classified as moderate with an index carbon index value of 1.79. Migratory birds found in the Lake Limboto area are 9 species of migratory birds belonging to 7 families with a total of 706 individuals. The highest index value H'= 1.63 is at station 1, the diversity index value is moderate and the lowest hidden index H'= 0 is at station 4, the index value is relatively low.
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Shaharuddin, Bakiah, Sajjad Ahmad, Simi Ali und Annette Meeson. „Limbal side population cells: a future treatment for limbal stem cell deficiency“. Regenerative Medicine 8, Nr. 3 (Mai 2013): 319–31. http://dx.doi.org/10.2217/rme.13.18.
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Ayuba, Iyan. „Pengaruh Atribut Produk Dan Pengetahuan Konsumen Terhadap Niat Pembelian Pada PT Hasjrat Abadi Cabang Limboto.“ Gorontalo Management Research 1, Nr. 1 (01.04.2018): 29. http://dx.doi.org/10.32662/gomares.v1i1.123.
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Iyan Ayuba, 2018 the effect of knowledge and attributes of products to customer friendly shopping at pt. hasjrat abadi cabang limboto. The purpose of this study was to determine how attributes influence consumer knowledge of the product and purchase intentions in PT Hasjrat Abadi cabang Limboto and the population in this study are all consumers who never make purchases at PT Hasjrat Abadi cabang Limboto. Samples were taken using purposive sampling sample size of 50 people . Data analysis technique used is multiple regression . The results showed that : 1 ) . Product attributes significantly influence purchase intention on PT Hasjrat Abadi cabang Limboto. 2 ) . Consumer knowledge significantly influence purchase intention on PT Hasjrat Abadi cabang Limboto.
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Polisetti, Naresh, Lyne Sharaf, Ursula Schlötzer-Schrehardt, Günther Schlunck und Thomas Reinhard. „Efficient Isolation and Functional Characterization of Niche Cells from Human Corneal Limbus“. International Journal of Molecular Sciences 23, Nr. 5 (02.03.2022): 2750. http://dx.doi.org/10.3390/ijms23052750.
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The fate decision of limbal epithelial progenitor cells (LEPC) at the human corneal limbus is determined by the surrounding microenvironment with limbal niche cells (LNC) as one of its essential components. Research on freshly isolated LNC which mainly include limbal mesenchymal stromal cells (LMSC) and limbal melanocytes (LM) has been hampered by a lack of efficient protocols to isolate and purify these cells. We devised a protocol for rapid retrieval of pure LMSC, LM and LEPC populations by collagenase digestion of limbal tissue and subsequent fluorescence-activated cell sorting (FACS) using antibodies against CD90 and CD117. The sorted cells were characterized by immunophenotyping and functional assays. The effects of LMSC and LM on LEPC were studied in 3D co-cultures and LEPC differentiation status was assessed by immunohistochemistry. Enzymatic digestion and flow sorting yielded pure populations of LMSC (CD117−CD90+), LM (CD117+CD90−), and LEPC (CD117−CD90−). The LMSC exhibited self-renewal capacity (55.0 ± 4.6 population doublings), expressed mesenchymal stem cell markers (CD73, CD90, CD105, and CD44), and transdifferentiated to adipocytes, osteocytes, or chondrocytes. The LM exhibited self-renewal capacity and sustained melanin production. The sorted LEPC expressed epithelial progenitor markers (CK14, CK19, and CK15) and showed a colony-forming ability. Co-cultivation of LMSC and LM with LEPC resulted in a 4–5-layered stratified epithelium and supported the preservation of a LEPC phenotype, as reflected by increased p63+ and Ki67+ cells and decreased CK12+ cells compared with LEPC monocultures. A highly efficient isolation of pure LM, LMSC, and LEPC populations from a single preparation may allow for direct transcriptomic and proteomic profiling as well as functional studies on native unpassaged LNC, which can be considered as proper equivalents of LNC in vivo. The developed biomimetic 3D co-culture method could provide an experimental model for investigating the functional role of LNC in the limbal stem cell niche.
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Abdul, Elfis Mus. „Pengaruh Persepsi Bauran Pemasaran Terhadap Kepuasan Pelanggan Di Percetakan Tiara Limboto Kabupaten Gorontalo“. Gorontalo Management Research 1, Nr. 1 (01.04.2018): 74. http://dx.doi.org/10.32662/gomares.v1i1.128.
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ELFIS MUS ABDUL, 2018. marketing hotchpotch perception influence towards customer satisfaction at Printing Office Tiara Limboto regency.This watchfulness aims to detect influence presepsi marketing hotchpotch towards customer satisfaction at Printing Office Tiara Limboto regency Gorontalo. Data that used in this watchfulness primary data that baseds on from admission filling kuisioner. Watchfulness population number 50 customers by using incedental method. Technics analysis that used to answer watchfulness problem and test hypothesis doubled regression analysis constructively software statistical package social science (spss) that is used to analyze data. Before do hypothesis test, done validity testing and reliabilitas instrument that continued pegujian regression analysis rules.This watchfulness finding (1) Found positive influence and significant Product towards customer satisfaction at Printing Office Tiara Limboto regency Gorontalo, (2) Found positive influence and significant Price towards customer satisfaction at printing office Tiara Limboto regency Gorontalo, (3) Found positive influence and significant Place towards customer satisfaction at Printing Office Tiara Limboto regency Gorontalo, (4) Found positive influence and significant Promotion towards customer satisfaction at Printing Office Tiara Limboto regency Gorontalo.Testing simultaneously attribute that product, price, place and promotion positive influential and significant towards customer satisfaction at printing office Tiara Limboto regency Gorontalo. Influential dominant variable towards customer satisfaction at Printing Office Tiara Limboto regency Gorontalo is Place.
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Liu, Shaohui, Jing Li, Chuanfu Wang, Donald Tan und Roger Beuerman. „Human Limbal Progenitor Cell Characteristics are Maintained in Tissue Culture“. Annals of the Academy of Medicine, Singapore 35, Nr. 2 (15.02.2006): 80–86. http://dx.doi.org/10.47102/annals-acadmedsg.v35n2p80.
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Introduction: Acute central serous chorioretinopathy (CSCR) afflicts young middle-aged males in the Western population. We aimed to analyse patient demographics and to determine the angiographic characteristics of acute CSCR in an Asian population. Materials and Methods: This is a retrospective study of all patients presenting with acute CSCR who had fundal fluorescein angiograms performed within a 4-year period (between 1 January 1998 and 31 December 2001). Results: The fluorescein angiograms of 128 patients were analysed. The majority were male (109/128) with a male-to-female ratio of 5.7:1. The age range of patients was 26 to 60 years, with a mean age of 41 years. The majority of patients (84%) were aged 30 to 50 years. With regard to racial distribution, 83% were Chinese, 6% were Malays and 11% were Indians or of other races. Unilateral disease was found in 74 patients (58%) and 52 had bilateral disease. The macula was the most common site of fluorescein leakage and was found in 97 patients (76%). Almost half the patients (44%) had more than one site of disease involvement (i.e., multifocal). The inkblot leakage pattern was found in 103 patients (80%). Conclusions: The patient demographics of acute CSCR in our population were compared to that reported in the West. The gender ratio was similar, with males being afflicted 6 to 10 times more compared to females. There was no racial predilection found for acute CSCR in the local population. We also found a significant proportion of patients with bilateral and multifocal disease compared to the West. The inkblot pattern of leakage was the most common pattern seen on angiography. There were a significant number of cases with bilateral and multifocal involvement, exceeding those reported in non-Asian populations. Key words: Cell culture, Feeder layer, Limbal epithelial cells, Progenitor cells, Stem cells
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Aprianto Sabihi, Nurfaika Nurfaika und Syahrizal Koem. „PEMANFAATAN TEKNOLOGI SISTEM INFORMASI GEOGRAFI UNTUK PEMETAAN POLA ALIRAN AIR TANAH DI KECAMATAN LIMBOTO“. Ocean Engineering : Jurnal Ilmu Teknik dan Teknologi Maritim 1, Nr. 4 (16.12.2022): 51–63. http://dx.doi.org/10.58192/ocean.v1i4.370.
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Limboto Subdistrict, which is the capital of Gorontalo Regency, has fairly high population growth and is an area that is vulnerable to the effects of drought. This causes the availability of groundwater to decrease. This study aims to utilize the GIS application to determine the pattern of groundwater flow in the Limboto District. This groundwater flow pattern can be used as a reference for further research related to groundwater. The type of data in this study is the type of primary data. The method used in this research is a survey method, namely by measuring directly in the field. The data analysis technique used to determine the pattern of groundwater flow in Limboto Regency is utilizing software (GIS) using ArcGIS 10.7 software to obtain a map of groundwater flow patterns in Limboto District. The results of the spatial analysis of the groundwater flow pattern map in Limboto District show that water flows from the northern part of the study site to the southern part of the study site.
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Tote, Sachin, Sanjay Wanjari und Darshana Tote. „ESTIMATION OF STATURE FROM UPPER LIMB ANTHROPOMETRY IN MAHARASHTRIANS POPULATION: A CADAVERIC STUDY“. International Journal of Anatomy and Research 5, Nr. 4.3 (01.12.2017): 4640–44. http://dx.doi.org/10.16965/ijar.2017.358.
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Langor, David W. „HOST EFFECTS ON THE PHENOLOGY, DEVELOPMENT, AND MORTALITY OF FIELD POPULATIONS OF THE MOUNTAIN PINE BEETLE, DENDROCTONUS PONDEROSAE HOPKINS (COLEOPTERA: SCOLYTIDAE)“. Canadian Entomologist 121, Nr. 2 (Februar 1989): 149–57. http://dx.doi.org/10.4039/ent121149-2.
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AbstractPhenology, fecundity, development, and mortality were studied for co-occurring, declining populations of Dendroctonus ponderosae Hopkins in limber pine and lodgepole pine at two sites in the Porcupine Hills of southwestern Alberta in 1985–1986. Beetles reared in lodgepole pine emerged and attacked new hosts 7–8 days earlier than those in limber pine in 1985. Beetles were able to utilize over two-thirds of the length of each limber pine bole but only about one-third of the length of each lodgepole pine bole. Also, beetles infesting limber pine had significantly higher fecundity, produced more eggs per centimetre of gallery length, and their progeny developed faster and survived better than beetles infesting lodgepole pine. There was no apparent phenological or other barrier that might inhibit gene flow between D. ponderosae populations in limber pine and lodgepole pine. In the area studied, limber pine was a better host for D. ponderosae reproduction, development, and survival than was lodgepole pine. Thus, beetle populations may be able to increase much more quickly in limber pine, arguing for regular monitoring of these populations.
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Junus, Nirwan, und Karlin Zakaria Mamu. „Limboto Lake Band Land Rights Arrangements“. Jambura Law Review 4, Nr. 2 (30.07.2022): 328–43. http://dx.doi.org/10.33756/jlr.v4i2.18795.
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This paper aims to examine the regulation of land use by the Limboto Lake community. This study uses an empirical juridical approach. The study results show that the regulation of land rights, especially land along the banks, has been regulated by the government and local governments, starting from laws and regulations, regional regulations, and other decisions. The existence of laws and regulations has given legitimacy to the state, in this case, the government, regarding the control and utilization of the land on the banks of the lake because the state legally owns the land on Lake Limboto. However, the fact is that the land on the banks of the lake has changed its function to become a community settlement. , making it difficult to carry out an inventory of the surrounding population. In order to prevent mismanagement or management that is not based on the rule of law, it is necessary to have clear and accurate boundaries regarding the allotment of areas/zones for various interests so that there is no overlap between the government and the land agency, considering that some community lands already have land rights (land certificate). Thus, an alternative solution to save the lake is establishing a zoning system.
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Tamaria, Savita, Davinder K. Gaur, Nidhi Kalra und Sumit Kalra. „Comparison of Lower Limb and Trunk Muscle Strength Training on Balance in Elderly Population“. Physiotherapy and Occupational Therapy Journal 9, Nr. 3 (2016): 103–7. http://dx.doi.org/10.21088/potj.0974.5777.9316.4.
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Putri, Gusti Ayu, Syamsidar Syamsidar, Yustisia Ali und Mansyur Tomayahu. „SARAPAN PAGI TERHADAP PRESTASI BELAJAR SISWA KELAS VIII SMP NEGERI 1 LIMBOTO“. Journal Nursing Care Jurusan Keperawatan Politeknik Kesehatan Gorontalo 6, Nr. 1 (10.11.2021): 37. http://dx.doi.org/10.52365/jnc.v6i1.339.
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ABSTRACT
Background: Education is one of importent factor to improve the quality of national future. Learning is a basic activity of all lebvel aducation. Purpose. The riset aims to relationship between breakfast and student achievement in class VIII SMP Negeri 1 Limboto. Methods: The study used a retrospective with a cross sectional study design. The population is 163 class VIII students of SMP Negeri 1 Limboto with a research sample of 116 respondents using random sampling technique. Results: Based on the results of the analysis of the relationship between breakfast and student achievement in class VIII SMP Negeri 1 Limboto using the chi-square correlation test with a calculated X2 value of 24.061 which is greater than X2 table. Conclusion: There is a relationship between breakfast and student achievement in class VIII
ABSTRAK
Latar Belakang: Pendidikan merupakan salah satu faktor penting untuk meningkatkan kualitas dari bangsa dimasa yang akan datang. Belajar merupakan kegiatan yang sangat mendasar dalam penyelenggaraan setiap jenis dan jenjang pendidikan. Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan sarapan pagi dengan prestasi belajar siswa kelas VIII SMP Negeri 1 Limboto. Metode: Penelitian menggunakan retrospektif dengan rancangan cross sectional study. Populasi sebanyak 163 siswa kelas VIII SMP Negeri 1 Limboto dengan sampel penelitian sebanyak 116 responden dengan menggunakan teknik sampling Random Sampling. Hasil: Berdasarkan hasil uji analisa hubungan sarapan pagi dengan prestasi belajar siswa kelas VIII SMP Negeri 1 Limboto menggunakan uji korelasi chi-square dengan nilai X2 hitung sebesar 24,061 lebih basar dari X2 tabel. Kesimpulan: Ada hubungan sarapan pagi dengan prestasi belajar siswa kelas VIII SMP Negeri 1 Limboto.
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Jääskeläinen, E., P. Juola, J. Kurtti, M. Haapea, M. Kyllönen, J. Miettunen, P. Tanskanen et al. „Associations between brain morphology and outcome in schizophrenia in a general population sample“. European Psychiatry 29, Nr. 7 (September 2014): 456–62. http://dx.doi.org/10.1016/j.eurpsy.2013.10.006.
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AbstractObjectiveTo analyse associations between brain morphology and longitudinal and cross-sectional measures of outcomes in schizophrenia in a general population sample.MethodsThe sample was the Northern Finland 1966 Birth Cohort. In 1999–2001, structural brain MRI and measures of clinical and functional outcomes were analysed for 54 individuals with schizophrenia around the age of 34. Sex, total grey matter, duration of illness and the use of antipsychotic medication were used as covariates.ResultsAfter controlling for multiple covariates, increased density of the left limbic area was associated with less hospitalisations and increased total white matter volume with being in remission. Higher density of left frontal grey matter was associated with not being on a disability pension and higher density of the left frontal lobe and left limbic area were related to better functioning. Higher density of the left limbic area was associated with better longitudinal course of illness.ConclusionsThis study, based on unselected general population data, long follow-up and an extensive database, confirms findings of previous studies, that morphological abnormalities in several brain structures are associated with outcome. The difference in brain morphology in patients with good and poor outcomes may reflect separable aetiologies and developmental trajectories in schizophrenia.
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Fadila, Nur, Nurfaika Nurfaika und Rusiyah Rusiyah. „Pemetaan Kebutuhan Air Domestik Masyarakat Di Kecamatan Limboto Provinsi Gorontalo“. Geosfera: Jurnal Penelitian Geografi 1, Nr. 1 (30.06.2022): 24–31. http://dx.doi.org/10.34312/geojpg.v1i1.14300.
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Domestic water has an important role to meet the needs of human life. The need and use of water always increase which is directly proportional to population growth. Limboto Subdistrict has increased every year, causing an increase in population, development and also the need for water is increasing both domestic, agricultural and plantation water needs. For this reason, planning and conservation related to water resources, especially groundwater, is important to study. The formulation or purpose in this paper is to analyze the spatial distribution of domestic water demand in eight villages in Limboto District. The method used in this study is the direct interview method to determine the water needs of the community, domestic water needs are analyzed using the standard calculation method for domestic water needs based on SNI 9-6728.1.202. The results of this study indicate that Hunggaluwa Village and Hutuo Village are areas that have high domestic water needs (QDMI) with a total of 633,477 (L/person/day) and 589,985 (L/person/day) while areas that have domestic water needs (QDMI) very low, namely in Kayu Merah Village with a total of 115,980 (L/person/day).
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Patanduk, Ricky, Wawan Budianta, Inacio Maria Deonal de Fatima und Darmawel Umar. „Application of DRASTIC method to identify the groundwater vulnerability to pollution in the sub-district of West Limboto and Limboto, Gorontalo Regency, Indonesia“. E3S Web of Conferences 479 (2024): 02003. http://dx.doi.org/10.1051/e3sconf/202447902003.
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The increment in population and agricultural sectors in West Limboto and Limboto, Gorontalo, affects the increased production of pollutants. The community uses shallow groundwater as the main water source. Since shallow groundwater is vulnerable to pollution due to community activity, this study aims to identify its vulnerability in West Limboto and Limboto. It was identified using the DRASTIC Method that will be integrated with Geographic Information System (GIS) Technique. The used parameters are Depth to Water Table, Recharge, Aquifer Media, Soil Media, Topography, Impact of Vadoze Zone, and Hydraulic Conductivity of the Aquifer. Depth to Water Table data is obtained from direct field measurement. Recharge amount data is obtained from the calculation of groundwater recharge. Aquifer Media, the Impact of Vadoze Zone, and Soil Media data are obtained from surface and subsurface geological data. Topography data is obtained from Digital Elevation Model Map. Hydraulic Conductivity is obtained from Pumping Test Data. The analysis result shows that the groundwater vulnerability of the study area was classified into 2 classes, the high index was in the southern part, and the low index was in the middle to the northern part of the study area.
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Sevgi, Mehmet Ali. „The Invisible Inhabitants of a Cultural Limbo“. Anthropology of the Middle East 18, Nr. 1 (01.06.2023): 20–34. http://dx.doi.org/10.3167/ame.2023.180103.
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Abstract This qualitative study, based on interviews and participant observation, is an effort to understand the religious identities of Turkish Shi'is (Ja'faris) living in Igdir by examining their rituals, their social and cultural lives, and their relations with Iran. The local population of Igdir consists of Shi'i Turks and Sunni Kurds. While the Turkish Shi'is living in the region meet on common ground with the majority of the country with their ethnic identities, they differ in their religious identities. It is possible to see these differences in the sociocultural life of the city, its religious rituals, and its multifaceted relations with Iran, the center of Shi'ism. The results of this research, which spanned a long period and included people from different parts of society, may constitute a basis for the further discussion of some points about the Ja'faris living in Igdir.
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de Paiva, Cintia S., Zhuo Chen, Rosa M. Corrales, Stephen C. Pflugfelder und De-Quan Li. „ABCG2 Transporter Identifies a Population of Clonogenic Human Limbal Epithelial Cells“. Stem Cells 23, Nr. 1 (Januar 2005): 63–73. http://dx.doi.org/10.1634/stemcells.2004-0093.
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Polisetti, Naresh, Thomas Reinhard und Günther Schlunck. „Efficient Isolation and Expansion of Limbal Melanocytes for Tissue Engineering“. International Journal of Molecular Sciences 24, Nr. 9 (25.04.2023): 7827. http://dx.doi.org/10.3390/ijms24097827.
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Limbal melanocytes (LMs) are found in the corneoscleral limbus basal epithelial layer and interact with neighboring limbal epithelial progenitor cells. The difficulty of isolating and cultivating LMs is due to the small fraction of LMs in the overall limbal population and the frequent contamination of primary cultures by other cell types. This has limited the research on freshly isolated LMs and the investigation of their biological significance in the maintenance of the limbal stem cell niche. Here, we describe an optimized protocol for the efficient isolation and expansion of LMs from cadaveric corneal limbal tissue using CD90 and CD117 as selective markers in fluorescence-activated cell sorting to obtain a pure population of LMs (CD90− CD117+) with self-renewal capacity and sustained melanin production. The isolation of pure LMs from a single preparation enables direct transcriptomic and proteomic analyses, as well as functional studies on freshly isolated LMs, which can be considered the proper counterparts of LMs in vivo and have potential applications in tissue engineering.
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Pakaya, Gita Regista, Dwi Nur Octaviani Katili und Siskawati Umar. „The Effect of Counseling using decision-making tools for family planning on interest in using the IUD“. Journal of Community Health Provision 1, Nr. 1 (27.02.2021): 31–37. http://dx.doi.org/10.55885/jchp.v1i1.27.
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Increasing the used of long-term contraception method of Intra Uterine Device (IUD)user required a good and quality counseling between the client and the provider (medical personnel). The research amis to determine the effect of counseling using decision-making tools for family planning on the interest in using IUDs in Dr. M.M. Dunda Hospital, Limboto District. The research type is Quasi Experiment, the population of all pregnant women who are admitted to Dr. M.M. Dunda Limboto. The sample was all 22 pregnant women who came in April 2020 which were divided into two groups, the intervention group and the control group. Sampling technique used accidental sampling. Data collected using a check list sheet and using chi square analysis. The results showed that X2 count> X2 table (6.6> 3,841) then H0 was rejected and Ha was accepted, so it could be concluded that there was an effect of counseling using ABPK on the interest in using IUDs in Dr. M.M. Dunda Hospotal, Limboto District.
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Habu, Azhar J., und Sumarni Simon. „PENGARUH PELAYANAN CUSTOMER SERVICE DALAM MENGATASI KELUHAN NASABAH PADA PT BANK SULUTGO CABANG LIMBOTO“. JEKSYAH (Islamic Economics Journal) 1, Nr. 1 (03.05.2021): 27–37. http://dx.doi.org/10.54045/jeksyah.v1i1.292.
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This study aims to determine and test the Effect of Customer Service Services in Overcoming Customer Complaints of Customer Satisfaction in P. Sulutgo Bank Limboto Branch. This type of research is quantitative research. The population in this study is the customer at P. Sulutgo Bank Limboto Branch. The sample used in this study was 100 customers. The data used are primary data obtained through a questionnaire that is processed using the IBM SPSS 25 application. Data analysis method used is a simple regression analysis. The results of this study indicate that there is a positive and significant influence of Customer Service on Customer Satisfaction at PT. Bank Sulutgo Limboto Branch with a significance value of 0.040. the value of t count is greater than t table for customer service variables of 2,077> 1,660 and a significance value of 0.040. This significance value is still smaller than the alpha value (0.05) so that H_0 is rejected.
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Habu, Azhar J., und Sumarni Simon. „PENGARUH PELAYANAN CUSTOMER SERVICE DALAM MENGATASI KELUHAN NASABAH PADA PT BANK SULUTGO CABANG LIMBOTO“. JEKSYAH (Islamic Economics Journal) 1, Nr. 1 (03.05.2021): 27–37. http://dx.doi.org/10.54045/jeksyah.v1i1.292.
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This study aims to determine and test the Effect of Customer Service Services in Overcoming Customer Complaints of Customer Satisfaction in P. Sulutgo Bank Limboto Branch. This type of research is quantitative research. The population in this study is the customer at P. Sulutgo Bank Limboto Branch. The sample used in this study was 100 customers. The data used are primary data obtained through a questionnaire that is processed using the IBM SPSS 25 application. Data analysis method used is a simple regression analysis. The results of this study indicate that there is a positive and significant influence of Customer Service on Customer Satisfaction at PT. Bank Sulutgo Limboto Branch with a significance value of 0.040. the value of t count is greater than t table for customer service variables of 2,077> 1,660 and a significance value of 0.040. This significance value is still smaller than the alpha value (0.05) so that H_0 is rejected.
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J. Habu, Azhar, und Sumarni Simon. „Pengaruh Pelayanan Customer Service Dalam Mengatasi Keluhan Nasabah Pada PT Bank Sulutgo Cabang“. JEKSYAH (Islamic Economics Journal) 1, Nr. 1 (03.05.2021): 27–37. http://dx.doi.org/10.54045/jeksyah.v1i1.7.
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This study aims to determine and test the Effect of Customer Service Services in Overcoming Customer Complaints of Customer Satisfaction in P. Sulutgo Bank Limboto Branch. This type of research is quantitative research. The population in this study is the customer at P. Sulutgo Bank Limboto Branch. The sample used in this study was 100 customers. The data used are primary data obtained through a questionnaire that is processed using the IBM SPSS 25 application. Data analysis method used is a simple regression analysis. The results of this study indicate that there is a positive and significant influence of Customer Service on Customer Satisfaction at PT. Bank Sulutgo Limboto Branch with a significance value of 0.040. the value of t count is greater than t table for customer service variables of 2,077> 1,660 and a significance value of 0.040. This significance value is still smaller than the alpha value (0.05) so that H_0 is rejected
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Tomy Gobel, Titin Dunggio und William Indra S. Mooduto. „THE INFLUENCE OF LEADERSHIP, WORK COMMITMENT AND ORGANIZATIONAL CULTURE ON EMPLOYEE PERFORMANCE AT THE BANK SULUTGO LIMBOTO“. Journal of Economic, Business, and Administration (JEBA) 2, Nr. 2 (21.04.2022): 18–27. http://dx.doi.org/10.47918/jeba.v2i2.232.
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This research was conducted at the Bank Sulutgo Limboto in November 2020. With the aim of research to determine the influence of leadership, organizational culture, work commitment on employee performance at the Bank Sulutgo Limboto.
This research was traced using multiple linear regression analysis method. The research location chosen was the Limboto Branch of Bank SulutGo in Gorontalo Regency and was carried out around October-November 2020. The study population was employees of Bank SulutGo Limboto branch with a total of 40 people. Sampling is done by means of saturated samples or census because the number of employees is 40 people so all of them are taken to represent research information. As for the research components to be studied are the dependent variable / dependent variable (performance of Bank SulutGo employees), and the independent variables / independent variables are (leadership, organizational culture, and work commitment). Primary data collection techniques by means of observation, interviews, and surveys using multiple regression analysis.
The results showed that there was a positive influence between leadership on employee performance of 3.103> 0.685 and p value of 0.004 <0.005, work commitment had an effect on the performance of Bank Sulutgo employees of 3.171> 0.685 and p value of 0.003 <0.005 and organizational culture had an effect on the performance of employees of Bank Sulutgo Limboto is 3.053> 0.685 and p value 0.004 <0.005, simultaneously the three variables contribute positively on the employees performance of the Bank Sulutgo Limboto based on the F-count value (20.430) greater than the F-table value (2.863) and p value 0.000 < 0.005.
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Hunta, Syafrudin. „EVALUASI PENURUNAN TINGKAT KEHILANGAN AIR KOTA LIMBOTO PADA PERUSAHAAN DAERAH AIR MINUM (PDAM) KABUPATEN GORONTALO“. Jurnal Teknik Sipil, Arsitek, Perencanaan Wilayah (J-TSIAP) 2, Nr. 2 (28.07.2023): 82–93. http://dx.doi.org/10.37971/j-tsiap.v2i2.126.
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The aim of this research is to determine the estimated magnitude of the increase in clean water demand and the service level of the Limboto Unit PDAM until 2020, as well as to evaluate the distribution of water loss levels in the Limboto City SPAM clean water network. The research method used is a case study by analyzing and evaluating the required data, including data on the number of residents, number of customers and data on the production and distribution of PDAM Limboto Unit water for the last 5 years. This research is divided into several activity stages, namely: projection of the number of consumers and amount of clean water usage, analysis of needs and level of clean water services until 2020 and evaluation of the distribution of water loss levels in PDAM Limboto City. The research results show that PDAM's water distribution capacity in 2020 to serve customer needs according to the estimated population is 229.55 lt/s out of a total number of consumers of 88,146 people. Service coverage in 2011 in Limboto City has reached 78.01%. The level of water loss that occurs in the Limboto City SPAM clean water supply system is estimated for the distribution network on average at 9.80% and for the transmission network on average at 25.94%. According to the results of the analysis and evaluation, activities are needed to increase the capacity of the transmission network and production system in stages of 40-80 liters/second, adding reservoirs, adding and replacing pipes and meters in line with the increasing number of customers and the need for clean water until 2020.
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Tätzsch, Kathryn. „Local Solutions for Displaced Populations – Alternatives to a “Transit”-Generation’s Time “in-limbo”“. Sicherheit & Frieden 34, Nr. 1 (2016): 52–59. http://dx.doi.org/10.5771/0175-274x-2016-1-52.
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Mano, Debby Hariyanti J., Hasim Hasim und Marini Susanti Hamidun. „Development of Ectourism In Limboto Lake Through Supply and Demand Approach“. Jambura Agribusiness Journal 4, Nr. 2 (31.01.2023): 84–93. http://dx.doi.org/10.37046/jaj.v4i2.17733.
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Limboto Lake is one of the lakes in Indonesia which is increasingly degraded due to uncontrolled sedimentation rates and aquatic plant populations, causing siltation. This study seeks to identify the ecotourism potential in Limboto Lake with a supply and demand approach. It applied questionnaires distribution, observation, and literature study method, while the analysis used is scoring and descriptive. Based on findings, Limboto Lake possesses supply aspects, including tourist attractions, facilities, accessibility, and community. For tourist attractions, there are a variety of lake activities that can be turned into ecotourism attractions, as well as the biodiversity of flora and fauna, art, and culture. In addition, the tourist facilities are still minim, which requires supporting facilities such as public toilets, homestays, information boards, and parking. Further, demand potential includes the characteristics, motivation, and tourists’ desires/demands. The tourists are dominated by women, and domestic tourists, with frequent visits. The main activity for a visit is lake boating, and the most preferred object is the natural scenery. Information about the potential for ecotourism with conservation principles can be one way to support efforts to save Limboto Lake.
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Alfiansyah, Muhammad Dimas, Azwar Thaib und Nurhayati Nurhayati. „Pengaruh Pengkayaan PUN – 11 (Perikanan Unaya 2011) dengan Limbah Sawi terhadap Jumlah Populasi Cacing Sutra (Tubifex sp.)“. Jurnal TILAPIA 4, Nr. 1 (31.01.2023): 75–82. http://dx.doi.org/10.30601/tilapia.v4i1.3656.
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The success of cultivation is influenced by several aspects, one of which is the natural food in the hatchery or fish larvae phase. One type of natural feed that is suitable for fish larvae is silk worms (Tubifex sp.). But the availability of silk worms is still very limited, so further culture is needed. This study aims to determine the effect of PUN-11 enrichment with mustard greens waste on the growth of silkworm populations. The design used in this study was a comparative design consisting of 2 treatments and 3 replications. The treatments used were P1 (PUN = 11%) and P2 (PUN = 11%) + mustard greens waste (10%). The results showed that the highest population of silk worms (Tubifex sp.) was obtained in treatment P2 (PUN: 11 90% + mustard greens waste 10%), which was 3105 ind/cm2, and the lowest number of silk worms was in treatment P1 (PUN: 11 100%), which is 2868 ind/cm2. The results showed that the T test showed that P2 had a significantly different effect (P > 0.05) on the silkworm population growth.
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Masaong, Abd Kadim, Sabirin B. Syukur und Mohamad Ariyanto Tuna. „Work Stress Management Nurse RSUD dr. M.M Dunda Limboto“. PROMOTOR 7, Nr. 1 (01.02.2024): 25–29. http://dx.doi.org/10.32832/pro.v7i1.494.
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Job or work-related stress is a response that a person may have when faced with demands and work pressures that are not their knowledge and ability to be able to handle it. The purpose of this study is to find out how the stress management of nurses at RSUD dr. M.M Dunda Limboto. This research method is quantitative research with a descriptive analysis research design. Data collection was carried out using questionnaire sheets with a population of 73 respondents. The number of respondents was taken using purposive sampling techniques. The data was obtained by statistically using frequency distribution. The results of nurses' work stress management with an individual approach with sufficient category 60.3%, organizational approach sufficient category 57.5%, and social approach sufficient category 50.7%. It is suggested that this study is expected to add references to health services on how stress management in nurses.
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Kusbianto, Dwi Erwin, Laura Yohana Sitompul, Ayu Puspita Sari und Bimantara Suryaridya Oetama. „Total Mikroba pada Tanah yang Dicemari Sludge Limbah Pabrik Kertas dengan Perlakuan Pupuk Guano“. Berkala Ilmiah Pertanian 6, Nr. 2 (31.05.2023): 43. http://dx.doi.org/10.19184/bip.v6i2.39126.
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There are 83 paper mills in Indonesia recorded by the Indonesian Pulp and Paper Association (APKI) spread throughout the archipelago. These paper companies produce products in the form of tissues, various types of paper, and other fiber-based products. Efforts to meet the needs of this raw material produce innovations in the form of using waste paper by separating the ink from the fibers contained in the paper, this separation process is known as the deinking process. Paper mill solid waste can be in the form of sludge, pith, and biosludge. Sludge is a factory solid waste originating from the settling of WWTP which consists of 90% solid and 10% liquid. Paper mill waste in the form of solid waste from sludge contains heavy metals such as Cd, Cr, Pb, Ni, Cu, and Zn. These metals can be overcome by bioremediation using guano fertilizer which is an organic material derived from bat droppings which is rich in phosphate. The purpose of this study was to determine the microbial population in soil contaminated with paper mill sludge waste treated with guano fertilizer. The parameters observed in this study were the total number of bacteria and fungi that survive in soil contaminated with paper mill waste as an indication that the land can still be planted with certain plants. This research was conducted at the Laboratory of Soil Biology, Laboratory of Soil and Land Fertility Agrotechnopark – Faculty of Agriculture – University of Jember. This study used a 3x3 two factorial experimental design with the basic pattern of Randomized Block Design (RAK), namely between the concentration of paper mill effluent and the dose of guano fertilizer, each treatment was repeated three times. The microbial population can be determined by analyzing total bacteria, total fungi. Soil that had been contaminated with paper mill waste was incubated with guano fertilizer, each combination was analyzed at 15 hst and 30 hst for total bacterial and total fungal populations. During the incubation period, irrigation was carried out using a sprayer to maintain moisture in the incubation media. The results showed that the total population of bacteria and fungi in the growing media contaminated with paper mill sludge waste by administering different doses of guano showed results that were not significantly different, but the total population tended to increase. with increasing incubation period. Pollution of sludge waste in the planting medium has a significant effect on increasing the total Fungi Population when compared to the control. The relationship between paper mill effluent pollution and guano fertilization is very low with a coefficient of determination of less than 0.5.
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Domngang Noche, Christelle, Pierre Rene Fotsing Kwetche, Thierry Tumameu, Ernest Tambo, Roger Moyou, Assumpta Lucienne Bella und Udo Schumacher. „Relationship between tropical endemic limbo-conjunctivitis and intestinal helminths in a population of Cameroonian children“. Cogent Medicine 4, Nr. 1 (01.01.2017): 1345587. http://dx.doi.org/10.1080/2331205x.2017.1345587.
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Masood, Faisal, Jin-Hong Chang, Anosh Akbar, Amy Song, Wen-Yang Hu, Dimitri T. Azar und Mark I. Rosenblatt. „Therapeutic Strategies for Restoring Perturbed Corneal Epithelial Homeostasis in Limbal Stem Cell Deficiency: Current Trends and Future Directions“. Cells 11, Nr. 20 (16.10.2022): 3247. http://dx.doi.org/10.3390/cells11203247.
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Limbal stem cells constitute an important cell population required for regeneration of the corneal epithelium. If insults to limbal stem cells or their niche are sufficiently severe, a disease known as limbal stem cell deficiency occurs. In the absence of functioning limbal stem cells, vision-compromising conjunctivalization of the corneal epithelium occurs, leading to opacification, inflammation, neovascularization, and chronic scarring. Limbal stem cell transplantation is the standard treatment for unilateral cases of limbal stem cell deficiency, but bilateral cases require allogeneic transplantation. Herein we review the current therapeutic utilization of limbal stem cells. We also describe several limbal stem cell markers that impact their phenotype and function and discuss the possibility of modulating limbal stem cells and other sources of stem cells to facilitate the development of novel therapeutic interventions. We finally consider several hurdles for widespread adoption of these proposed methodologies and discuss how they can be overcome to realize vision-restoring interventions.
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Sniezko, Richard A., Robert Danchok, Douglas P. Savin, Jun-Jun Liu und Angelia Kegley. „Genetic resistance to white pine blister rust in limber pine (Pinus flexilis): major gene resistance in a northern population“. Canadian Journal of Forest Research 46, Nr. 9 (September 2016): 1173–78. http://dx.doi.org/10.1139/cjfr-2016-0128.
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Limber pine, Pinus flexilis E. James, a wide-ranging tree species in western North America, is highly susceptible to white pine blister rust (WPBR), caused by the non-native fungal pathogen Cronartium ribicola J.C. Fisch. The Canadian populations in particular have been heavily impacted, and in 2014, limber pine was designated endangered in Canada by the Committee on the Status of Endangered Wildlife in Canada (COSEWIC). Little is known about genetic resistance to WPBR in limber pine, but major gene resistance (MGR) has been characterized in some populations in the United States. This study examines resistance in seedling families from 13 parent trees from British Columbia, Alberta, and Oregon, representing the northern- and northwestern-most populations. Most families were susceptible, with 100% of the seedlings cankered, but one family from Alberta segregated 1:1 for cankered and canker free. This is the first report of (a) MGR in Canada of any of the four species of five-needle pines native to Canada and (b) any resistance in limber pine in Canadian populations and is the northernmost known incidence of putative R-gene resistance in a natural stand of any five-needle pine species. Many of the Canadian selections were from stands with high incidence of WPBR infection, and their high susceptibility in this trial suggests that further infection and mortality is likely in the Canadian populations.
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Wahyono, Sri. „KEBIJAKAN PENGELOLAAN LIMBAH ELEKTRONIK DALAM LINGKUP GLOBAL DAN LOKAL = Electronic Waste Management Policies in the Scope of Global and Local“. Jurnal Teknologi Lingkungan 14, Nr. 1 (01.12.2016): 49. http://dx.doi.org/10.29122/jtl.v14i1.1437.
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In line with population growth and lifestyle, the number of electronic waste increased. Electronicwaste is a hazardous waste that threaten human survival. Various efforts have been and are beingtaken to prevent environmental degradation caused by poor management of electronic waste.However, electronic waste is a source of valuable material that can be recovered through a series ofprocesses in the manufacturing of electronic waste. Support from various parties necessary for therealization of a sustainable e-waste management.Keywords: electronic waste, pollution, extended producer responsibilityAbstrakSejalan dengan pertumbuhan penduduk dan gaya hidup, limbah elektronik semakin meningkatjumlahnya. Limbah elektronik secara umum merupakan limbah berbahaya sehingga dapatmengancam kelangsungan hidup manusia. Berbagai upaya telah dan sedang dilakukan untukmencegah terjadinya degradasi lingkungan akibat pengelolaan limbah elektronik yang buruk. Namundemikian, limbah elektronik adalah sumber material berharga yang dapat di-recovery melaluiserangkaian proses di industri pengolahan limbah elektronik. Dukungan dari berbagai pihakdiperlukan untuk terwujudnya pengelolaan limbah elektronik yang berkelanjutan.Kata Kunci: Limbah elektronik, pencemaran, extended producer responsibility
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Mehlman, Max L., Shawn S. Winter, Stephane Valerio und Jeffrey S. Taube. „Functional and anatomical relationships between the medial precentral cortex, dorsal striatum, and head direction cell circuitry. I. Recording studies“. Journal of Neurophysiology 121, Nr. 2 (01.02.2019): 350–70. http://dx.doi.org/10.1152/jn.00143.2018.
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Head direction (HD) cells fire as a function of the animal’s directional heading and provide the animal with a sense of direction. In rodents, these neurons are located primarily within the limbic system, but small populations of HD cells are found in two extralimbic areas: the medial precentral cortex (PrCM) and dorsal striatum (DS). HD cell activity in these structures could be driven by output from the limbic HD circuit or generated intrinsically. We examined these possibilities by recording the activity of PrCM and DS neurons in control rats and in rats with anterodorsal thalamic nucleus (ADN) lesions, a manipulation that disrupts the limbic HD signal. HD cells in the PrCM and DS of control animals displayed characteristics similar to those of limbic HD cells, and these extralimbic HD signals were eliminated in animals with complete ADN lesions, suggesting that the PrCM and DS HD signals are conveyed from the limbic HD circuit. Angular head velocity cells recorded in the PrCM and DS were unaffected by ADN lesions. Next, we determined if the PrCM and DS convey necessary self-motion signals to the limbic HD circuit. Limbic HD cell activity recorded in the ADN remained intact following combined lesions of the PrCM and DS. Collectively, these experiments reveal a unidirectional functional relationship between the limbic HD circuit and the PrCM and DS; the limbic system generates the HD signal and transmits it to the PrCM and DS, but these extralimbic areas do not provide critical input or feedback to limbic HD cells. NEW & NOTEWORTHY Head direction (HD) cells have been extensively studied within the limbic system. The lesion and recording experiments reported here examined two relatively understudied populations of HD cells located outside of the canonical limbic HD circuit in the medial precentral cortex and dorsal striatum. We found that HD cell activity in these two extralimbic areas is driven by output from the limbic HD circuit, revealing that HD cell circuitry functionally extends beyond the limbic system.
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Malyutina, Darya. „Russian Journalists in Ukraine: Caught in Limbo?“ Russian Politics 2, Nr. 1 (09.03.2017): 98–122. http://dx.doi.org/10.1163/2451-8921-00201006.
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Since the 2004 Orange Revolution, Ukrainian media have provided a number of Russian journalists an alternative place to continue their career, whether they were looking for a comparatively free and pluralistic media space, a new job, or safety. The number of Russian media professionals in Ukraine increased in the subsequent years. Euromaidan and the annexation of Crimea in 2014, followed by Russia’s military intervention in Donbas, have contributed to decisions to move or extend their stay in Ukraine. However, these events have also complicated the position of Russian journalists in Ukraine. This paper seeks to explore the challenges connected with being a Russian journalist and working in Ukraine-based media during Russia’s hybrid war against Ukraine. This population of migrant media workers has arrived from an aggressor country, where mainstream media have been producing manipulative anti-Ukrainian discourse. They are diverse in terms of social backgrounds and migration histories, but mostly are qualified and experienced professionals. They do not form a tightly knit migrant community, and do not work for media outlets targeting such a community. This article addresses the experiences of a number of these media personalities, drawing upon a series of interviews conducted in late 2015, and open source materials. I argue that the Maidan, the annexation of Crimea, and the ongoing conflict have had a significant impact on Russian migrant journalists in Ukraine, by providing a migration context, influencing their work ethics and making them particularly sensitive to the ideas of responsibility and journalistic subjectivity.
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Rauf, Rahmat, M. Ramoend Manahung und Yuwin R. Saleh. „Pengaruh Media Dakota Terhadap Hasil Belajar Siswa Pada Pembelajaran Matematika FPB Dan KPK“. EDUCATOR (DIRECTORY OF ELEMENTARY EDUCATION JOURNAL) 2, Nr. 2 (21.12.2021): 139–51. http://dx.doi.org/10.58176/edu.v2i2.165.
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This study was conducted to find out how big the influence of Dakota Media on Student Learning Outcomes in Mathematics Learning of FPB and KPK in Class IV SDN 11 Limboto, Gorontalo Regency. The type of this research is a quantitative research with a Pre-Experimental approach, type One Group Pre-test Post-test Design. Determination of the population and samples taken from class IV totaling 30 students where all the population was sampled (saturated sampling). In collecting data, the author uses a multiple choice test with a total of 20 questions. From the results of the analysis of hypothesis testing, the results of the paired sample test were obtained with the final data obtained on the results of the Pre-test and Post-test, the value of t_count was 14,278 with df = 29 and t_table with a level of = 0.05 of 2,045. Thus, t_count 14,278 > t_table 2,045 which means that H1 is accepted and H0 is rejected with the result that there is an effect of Dakota Media on student learning outcomes in mathematics learning at FPB and KPK in fourth grade at SDN 11 Limboto, Gorontalo Regency.
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Sriana, Hemy, und Sarifah Noor Farah. „PERBANDINGAN PERTUMBUHAN EKSPLAN PADA PENGGUNAAN AIR LIMBAH AC (AIR CONDITONER) DENGAN AKUADES DALAM MEDIA SUBKULTUR DURIAN LAHUNG (Durio dulcis)“. EnviroScienteae 19, Nr. 4 (28.11.2023): 131. http://dx.doi.org/10.20527/es.v19i4.17818.
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Durian lahung (Durio dulcis) is one of the germplasm of Kalimantan which is becoming rare. One method of propagation with the advantage of obtaining lots of seeds, simultaneously and free from pests is through in vitro technique. One of the obstacles encountered in this process in the laboratory is the use of large amounts of aquades (distilled water), so alternative water is needed with almost the same characteristics so that it does not interfere the growth of explants, namely AC wastewater. The aim of this research is to compare the growth results of Lahung durian explants in a second subculture medium using AC wastewater which is unused waste with distilled water. The experimental design uses the t test by comparing 2 independent populations. The sample consists of a population from subculture result on media with an AC wastewater source and a population on distilled water. Each population consists of 10 replications and each replication consists of 5 bottles, so there are 100 planting bottles. The observations were made on the variables of contamination percentage (%), life percentage (%), browning percentage (%), number of roots, number of shoots, number of leaves, number of plumules, and shoot height. The results of the observations at 6 weeks after subculture showed that the explants from both treatments did not experience contamination, browning, and root growth, and 100% of the explants were alive. Meanwhile, the number of leaves, the number of plumules, shoots, nodes and shoot height were not significantly different between the two treatments.
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Latta, Robert G., und Jeffry B. Mitton. „A Comparison of Population Differentiation Across Four Classes of Gene Marker in Limber Pine (Pinus flexilis James)“. Genetics 146, Nr. 3 (01.07.1997): 1153–63. http://dx.doi.org/10.1093/genetics/146.3.1153.
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We examined genetic differentiation among seven populations of limber pine using four classes of gene marker. Among-population differentiation was much higher for maternally inherited mitochondrial DNA polymorphisms than for paternally inherited chloroplast DNA, indicating that wind-dispersed pollen is the main agent of gene flow. Chloroplast DNA differentiation is consistent with gene flow estimated in a prior paternity analysis. Using the estimates of seed and pollen flow derived from mtDNA and cpDNA differentiation, we predicted the value of Fst expected at nuclear loci. Allelic frequency differentiation at seven allozyme loci was relatively homogeneous across loci and consistent with the level of differentiation predicted from the organellar haplotypes. By contrast four of the nine randomly applied polymorphic DNA (RAPD) markers we examined were more strongly differentiated than this prediction, suggesting the action of diversifying selection. However, the differentiated RAPDs and mtDNA were concordant in dividing the populations into two groups, suggesting some historical division. Simulations show that such historical division can increase the interlocus variance in Fst but neither a historical nor an equilibrium model could account for the joint distribution of Fst estimates across both allozyme and RAPD loci. Thus at least one group of loci appears to be experiencing natural selection.
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Verma, Sudhir, Xiao Lin und Vivien J. Coulson-Thomas. „The Potential Reversible Transition between Stem Cells and Transient-Amplifying Cells: The Limbal Epithelial Stem Cell Perspective“. Cells 13, Nr. 9 (25.04.2024): 748. http://dx.doi.org/10.3390/cells13090748.
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Stem cells (SCs) undergo asymmetric division, producing transit-amplifying cells (TACs) with increased proliferative potential that move into tissues and ultimately differentiate into a specialized cell type. Thus, TACs represent an intermediary state between stem cells and differentiated cells. In the cornea, a population of stem cells resides in the limbal region, named the limbal epithelial stem cells (LESCs). As LESCs proliferate, they generate TACs that move centripetally into the cornea and differentiate into corneal epithelial cells. Upon limbal injury, research suggests a population of progenitor-like cells that exists within the cornea can move centrifugally into the limbus, where they dedifferentiate into LESCs. Herein, we summarize recent advances made in understanding the mechanism that governs the differentiation of LESCs into TACs, and thereafter, into corneal epithelial cells. We also outline the evidence in support of the existence of progenitor-like cells in the cornea and whether TACs could represent a population of cells with progenitor-like capabilities within the cornea. Furthermore, to gain further insights into the dynamics of TACs in the cornea, we outline the most recent findings in other organ systems that support the hypothesis that TACs can dedifferentiate into SCs.
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Umemoto, Terumasa, Masayuki Yamato, Kohji Nishida, Joseph Yang, Yasuo Tano und Teruo Okano. „Limbal Epithelial Side-Population Cells Have Stem Cell-Like Properties, Including Quiescent State“. Stem Cells 24, Nr. 1 (Januar 2006): 86–94. http://dx.doi.org/10.1634/stemcells.2005-0064.
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Tomback, Diana F., Anna W. Schoettle, Kristen E. Chevalier und Cheri A. Jones. „Life on the edge for limber pine: Seed dispersal within a peripheral population“. Écoscience 12, Nr. 4 (Januar 2005): 519–29. http://dx.doi.org/10.2980/i1195-6860-12-4-519.1.
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Amin, Sohil, Elmira Jalilian, Eitan Katz, Charlie Frank, Ghasem Yazdanpanah, Victor H. Guaiquil, Mark I. Rosenblatt und Ali R. Djalilian. „The Limbal Niche and Regenerative Strategies“. Vision 5, Nr. 4 (22.09.2021): 43. http://dx.doi.org/10.3390/vision5040043.
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The protective function and transparency provided by the corneal epithelium are dependent on and maintained by the regenerative capacity of limbal epithelial stem cells (LESCs). These LESCs are supported by the limbal niche, a specialized microenvironment consisting of cellular and non-cellular components. Disruption of the limbal niche, primarily from injuries or inflammatory processes, can negatively impact the regenerative ability of LESCs. Limbal stem cell deficiency (LSCD) directly hampers the regenerative ability of the corneal epithelium and allows the conjunctival epithelium to invade the cornea, which results in severe visual impairment. Treatment involves restoring the LESC population and functionality; however, few clinically practiced therapies currently exist. This review outlines the current understanding of the limbal niche, its pathology and the emerging approaches targeted at restoring the limbal niche. Most emerging approaches are in developmental phases but show promise for treating LSCD and accelerating corneal regeneration. Specifically, we examine cell-based therapies, bio-active extracellular matrices and soluble factor therapies in considerable depth.
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Claudia, Juli Gladis, und Wirdawaty S. Adam. „Efektifitas Senam Hamil terhadap Kejadian Rupture Perineum pada Ibu Bersalin di Puskesmas Limboto“. Gorontalo Journal of Public Health 1, Nr. 1 (19.04.2018): 053. http://dx.doi.org/10.32662/gjph.v1i1.152.
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Exercise in pregnancy have positive effect on the pregnancy outcomebecause stumulate to strengthen and maintain elasticity in abdominalmuscles and pelvic muscles which was associated with labor that whenstraining the muscles will relax actively that the head baby will outeasily and the pelvic floor muscle is weak, will not easily tear when willgive birth. The incidence of perineum rupture at Limboto CommunityHealth Center is 46,7%. The purpose of this study was to analyze theeffectiveness of gymnastics pregnancy against rupture perineum inMaternity at Limboto Community Health Center. The method of researchwas used pre-experimental research with statistical group comparisondesign with using chi square test. The population in this study were allthirty pregnant trimester III which amounted 30 people. The sample inthis study were 15 case groups and 15 control groups. The variablepregnant exercise was independent variabel and the dependentvariable was incidence of perineal rupture. The result of this researchby using chi square statistic test was showed t arithmetic 8,571, thatmean the respondent who have high pregnancy exercise did notexperience rupture perineum. Results p - value 0.009 with degree ofsignificance 0.05 (p < 0.05). The conclusion there was a significant effectof pregnancy exercise effectiveness on the incidence of perineal rupturein maternal mothers at Limboto Community Health Center.
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Zerger, Suzanne, Katherine Francombe Pridham, Erin Plenert, Caitlin Newberry, Adam Whisler, Indira Fernando und Naveed Ahmed. „Research Assistants Caught in Limbo: Considering Their Role in Quantitative, Longitudinal Research with Vulnerable Populations“. Journal of Health Care for the Poor and Underserved 26, Nr. 4 (2015): 1391–400. http://dx.doi.org/10.1353/hpu.2015.0107.
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PRADANA, ANKARDIANSYAH PANDU, DIANA PUTRI und ABDUL MUNIF. „Potensi Seduhan Limbah Baglog Jamur Tiram (Pleurotus ostreatus) dalam Menekan Populasi Radopholus similis dan Meloidogyne spp. pada Tanah Asal Perakaran Tanaman Lada (Piper nigrum L)“. Jurnal Sumberdaya Hayati 2, Nr. 2 (02.03.2017): 39–44. http://dx.doi.org/10.29244/jsdh.2.2.39-44.
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Indonesia is one of the largest pepper producing countries in the world. One of the pepper-producing provinces in Indonesia is the Bangka Belitung Islands (Babel). However, the infection of Radopholus similis and Meloidogyne spp. which causes yellow disease becomes one of obstacles in pepper cultivation in Babel. Thus, alternative solution to overcome this yellow disease is by reducing the amount of nematode inoculum of R. similis and Meloidogyne spp. in soil. This study aimed to determine the effectiveness of the infusion of oyster mushrooms baglog waste enriched with molasses to control the population of R. similis and Meloidogyne spp. and to increase the population of rhizobacteria. Infusion of oyster mushrooms baglog waste was mixed with 1% of molasses and was poured into soil obtained from the pepper plant roots. The concentrations used were 10%, 20%, 30% and 50%. Population of phytonematode, rhizobacteria, proteolytic bacteria, and group of fluorescence bacteria were calculated before and 7 days after treatment. Results showed that the populations of R. similis and Meloidogyne spp. in the soil poured with the influsion of oyster mushroom baglog waste decreased by 29.11% (R. similis) and 24.61% (Meloidogyne spp.), compared to the before and control treatments. The suppression of nematode population was found to be the highest in the influsion treatment at concentration of 50%. Overall, treatment of all concentrations succeeded to increase the population of rhizobacteria, proteolytic bacteria, and group of fluorescence bacteria in soil. Moreover, the highest increase was found in soil treated with concentration of 50%. This study provided new information that the infusion application of oyster mushrooms baglog waste enriched with molasses had the potential to increase the population of rhizobacteria and suppress the amount of pathogens R. similis and Meloidogyne spp.
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Edward Efendi Silalahi und Siti Nurmayanti. „PENGARUH BEBAN KERJA DAN KOMPENSASI TERHADAP KINERJA PEGAWAI PADA BADAN LAYANAN UMUM DAERAH PENGELOLAAN AIR LIMBAH DOMESTIK (BLUD PALD) KOTA BEKASI“. Juremi: Jurnal Riset Ekonomi 2, Nr. 5 (26.03.2023): 667–76. http://dx.doi.org/10.53625/juremi.v2i5.5332.
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This study aims to determine whether there is an effect of workload and compensation on employee performance at the Badan Layanan Umum Daerah Pengelolaan Air Limbah Domestik Kota Bekasi, either partially or simultaneously. This study uses quantitative methods, where primary data is obtained from the distribution of questionnaires. The population in this study were all employees of the Badan Layanan Umum Daerah Pengelolaan Air Limbah Domestik Kota Bekasi, totaling 63 employees, and the sample taken by all employees was 63 people, the technique used was saturated sampling. Saturated sampling is a sampling technique where all members of the population are used as samples, other terms are saturated samples or censuses. The results showed that partially the workload variable had a positive and significant effect on employee performance. Partially, the compensation variable has a positive and significant effect on employee performance. And simultaneously Workload and Compensation have a positive and significant effect on Employee Performance at the Badan Layanan Umum Daerah Pengelolaan Air Limbah Domestik (BLUD PALD) Kota Bekasi
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I. Biahimo, Nur Uyuun, Firmawati Firmawati, Inne Ariane Gobel und Aldawati A. Husuna. „Risk Factors for Bullying in Adolescents at SMA Negeri 1 Limboto“. PROMOTOR 7, Nr. 1 (01.02.2024): 34–41. http://dx.doi.org/10.32832/pro.v7i1.508.
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Bullying is an act of physical and verbal violence when the bully degrades and threatens the victim so that the victim is unable to resist. Bullying behavior has a negative impact, very disturbing and dangerous for adolescents if it is not immediately addressed. The purpose of this study was to determine the risk factors for bullying in adolescents at SMA Negeri 1 Limboto. This study used an analytical survey design, a population of 645 students of grades X and XI of SMA Negeri 1 Limboto, a sample of 86 students of grades X and XI of SMA Negeri 1 Limboto, the type of static random sampling sample, the instrument used was a questionnaire, the statistical test used in this study was a logistic regression test and using univariate analysis and bivariate analysis. The results obtained were the risk factors that most influenced bullying behavior, namely family factors with an R Square value of 74%, then media factors with an R Square value of 56%, and self-control bullying factors with an R Square value of 32%. The conclusion is that family factors are the most risky factors for bullying with the highest R square value, the suggestion from this study is that the better the family parenting style, the impact on the behavior produced by the child himself.