Thematische Bibliographien / Laboratory manipulation

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Auswahl der wissenschaftlichen Literatur zum Thema „Laboratory manipulation“

Autor: Grafiati

Veröffentlicht am 28. Juni 2021

Zuletzt aktualisiert am 1. Februar 2022

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Zeitschriftenartikel zum Thema "Laboratory manipulation"

Abouhilal, Abdelmoula, Amine Moulay Taj, Naima Taifi und Abdessamad Malaoui. „Using Online Remote Laboratory in Agriculture Engineering and Electronic Training“. International Journal of Online and Biomedical Engineering (iJOE) 15, Nr. 06 (29.03.2019): 66. http://dx.doi.org/10.3991/ijoe.v15i06.9699.

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<p class="0abstract">Practical manipulations are a core part of engineering training education systems. Remote labs are a new method used for teaching and practicing experimental manipulation using the performance of information and communication technologies. This paper presents a study of two remote labs architecture using low cost embedded systems that could be addressed to the 3rd year bachelor degree students on renewable energy and others on electronics courses. The first manipulation is based on Arduino microcontroller to monitor an irrigation system powered by photovoltaic panels. In addition, the second manipulation uses a powerful PcDuino, to control remotely a logic electronic experience. A simple interface is developed to allow students and instructors to access to these manipulations. This study is aimed to improve the present education systems in the Moroccan universities by managing the practical manipulation for a large number of students, especially in the open-access faculties. Finally, this architecture can be easily extended to other disciplines and courses.</p>

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Hsieh, Mu-Cheng, und Kuu-Young Young. „Effective manipulation for a multi-DOF robot manipulator in laboratory environments“. Journal of the Chinese Institute of Engineers 36, Nr. 5 (Juli 2013): 566–76. http://dx.doi.org/10.1080/02533839.2012.737112.

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Lasky, Larry C. „Hematopoietic Stem Cell Manipulation in the Laboratory“. Laboratory Medicine 18, Nr. 12 (01.12.1987): 843–45. http://dx.doi.org/10.1093/labmed/18.12.843.

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Bulfield, Grahame. „Genetic manipulation of laboratory and farm animals“. Journal of Chemical Technology & Biotechnology 43, Nr. 4 (24.04.2007): 265–72. http://dx.doi.org/10.1002/jctb.280430405.

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Parish, J. H. „Genetic Manipulation of Streptomyces — A Laboratory Manual“. Biochemical Education 14, Nr. 4 (Oktober 1986): 196. http://dx.doi.org/10.1016/0307-4412(86)90228-1.

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Ritchie, D. A. „Genetic manipulation of Streptomyces. A laboratory manual“. Endeavour 11, Nr. 1 (Januar 1987): 55. http://dx.doi.org/10.1016/0160-9327(87)90187-6.

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Bourlioux, P. „Genetic manipulation of Streptomyces: A laboratory manual“. Biochimie 69, Nr. 1 (Januar 1987): 82. http://dx.doi.org/10.1016/0300-9084(87)90278-1.

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Shopova, Dobromira, und Diyan Slavchev. „Laboratory investigation of Accuracy of Impression Materials for Border Molding“. Folia Medica 61, Nr. 3 (30.09.2019): 435–43. http://dx.doi.org/10.3897/folmed.61.e39351.

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Introduction: Border molding of the edge of the individual impression tray is an important stage of prosthetic treatment of edentulous jaws, which often depends on the final result of the treatment. Classical thermoplastic impression materials for border molding have positive qualities that make them preferable by clinicians for their hardness, unlimited manipulation time and high impression sharpness. Modern silicone impression materials for border molding have long manipulating time and appropriate viscosity to allow dentists to perform functional tests. Aim: To determine the accuracy of different impression materials for border molding of individual impression trays. Materials and methods: Four impression materials for border molding were laboratory tested: Kerr impression compound green sticks and thermoplastic GC Iso functional sticks, additive type silicone Detaseal function and condensation type silicone sta-seal f. A modified individual impression tray designed by authors was used, allowing for laboratory load and stability. Ten impressions were taken and their formed edges were measured at 10 points three times - immediately after hardening/elasification, and 24 hours and 48 hours after hardening/elasification. Results: The results were analysed using ANOVA repetition analysis, where a statistically insignificant difference in the accuracy of three of the impression materials for border molding was established, except the C-type of silicone. Conclusions: Good manipulative qualities and measured accuracy in laboratory tests define these materials as very good for border molding procedures.

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Crescimanno, A. „Transposition of standard JACIE in the laboratory of manipulation“. Drugs and Cell Therapies in Hematology 1, Nr. 1 (31.10.2012): 75. http://dx.doi.org/10.4081/dcth.2012.14.

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Bull, D. C., und E. K. Williams. „Chemical Changes in an Estuarine Sediment During Laboratory Manipulation“. Bulletin of Environmental Contamination and Toxicology 68, Nr. 6 (01.06.2002): 852–61. http://dx.doi.org/10.1007/s00128-002-0033-6.

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Dissertationen zum Thema "Laboratory manipulation"

Nevřiva, Václav. „Inteligentní manipulace s laboratorními objekty pomocí robotu ABB YuMi“. Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2021. http://www.nusl.cz/ntk/nusl-442858.

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The aim of the master thesis is to design a laboratory station and a control program operated by a collaborative robot IRB 14000 YuMi using an integrated effector camera to identify laboratory objects and control the progress of the task. In the introductory part, collaborative robots are briefly introduced, the IRB 14000 on which the task is implemented and the RobotStudio development environment together with the IntegratedVision extension are described in more detail. The following chapters describe the laboratory task itself, its solution and testing of the designed program.

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Ng, Koon-kiu, und 吳官橋. „Using zebrafish as a model organism for the study of embryonic hematopoiesis based on chemical screening and genetic manipulation“. Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hub.hku.hk/bib/B5071322X.

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Zebrafish has emerged as an important model for the study of embryonic hematopoiesis. It is a well-characterized model with numerous advantages. Large amount of embryos can be produced by a single pair of zebrafish and the optically transparent embryos allow direct visualization and manipulation of embryonic processes. Large-scale chemical screening using zebrafish embryos can be developed for robust screening of chemical libraries. The zebrafish hematopoiesis resembles that of mammals and occurs in two successive waves, primitive and definitive hematopoiesis. High-throughput read-outs are available to study the effects of different chemicals and genetic modifications on hematopoiesis. In first part of this study, an initial screening using O-dianisidine staining and whole-mount in-situ hybridization as read-out for chemicals that might perturb the regulation of hematopoiesis was conducted. Positive hit was further evaluated by flow cytometry of dissociated transgenic Tg(gata1:GFP) zebrafish embryos. A total of 50 compounds were screened from the "Mechanistic set" chemical libraries obtained from Developmental Therapeutics Program of the National Cancer Institute. One compound, "NSC 643834" was shown to reduce O-dianisidine staining at different concentrations tested. The second part of this study was performed to investigate the role of inca2 in zebrafish hematopoiesis. inca2 was found to be upregulated in chordin morphant zebrafish in which primitive hematopoiesis was expanded. The spatial expression of inca2 was examined by whole mount in-situ hybridization of embryos at different developmental stages. Furthermore the function of inca2 was investigated by gene knockdown using inca2 anti-sense morpholino. Primitive hematopoiesis was perturbed, suggesting that inca2 might play an important role in the regulation of this process. In conclusion, the present study demonstrated the distinct advantages and feasibility of using zebrafish as a platform of high throughput chemical screening and genetic manipulation. The result provided important ground to investigate the regulatory mechanisms of embryonic hematopoiesis.
published_or_final_version
Medicine
Master
Master of Research in Medicine

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Moral, Zamora Beatriz del. „Bioimpedance & dielectrophoresis instrumentation equipments for living cells manipulation and monitoring“. Doctoral thesis, Universitat de Barcelona, 2016. http://hdl.handle.net/10803/395178.

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Since the first microfluidic device was developed in the early 1950s, when the basics for today’s inkjet technology were set, thousands of publications have appeared related to the topic. The increasing interest on these technologies is caused by its ability to be scaled and its rapid development, which allows manipulating and detecting small quantities of analites even at the cellular scale. The integration of microfluidic technologies with specific sensors and actuators at minute scales in order to achieve a set of automated laboratory operations and perform a particular solution for a specific application, generally on the life sciences and chemistry fields, was defined as Lab-on-a-chip (LoC). LoC devices have the potential to become a powerful technology for some fields, such as health, food security or environmental control. Their low cost and portability make them also suitable to improve medical diagnosis and research in developing countries. Moreover, these systems permit also to explore new methods for manipulation and characterization of cells by means of electrical cell properties, by using techniques such as dielectrophoresis (DEP) or impedance spectroscopy (IS). In fact, the dielectrophoretic force allows manipulating cells, taking advantage of their electrical properties, by applying an electric field. Likewise, impedance allows measuring electrical properties of materials and, used wisely, inform about characteristics such as presence, composition or size of cells or other biological materials. This work aims, in its final stage, to exploit the combined potential of both techniques, DEP and IS, in a compact system for bioanalytical bench-top applications. The creation of the complete device has been a long procedure alternating theoretical calculations and experimental tests. It has included different steps such as the design of the need electronic equipment stages, the study of different microfluidic designs, an accurate bacteria concentration and manipulation protocol definition, and the study of the viability of the bacteria populations recovered with our device. These studies have made possible to finally obtain an automated bacteria concentrator for microbiology, food, water and environmental control applications while performing impedance cell analysis to monitor bacteria accumulation during the process. The system has been adjusted and proved for the real case of Escherichia Coli (E. coli) concentration and analysis. E. coli presents pathogenic variants that cause morbidity and mortality worldwide being therefore a topic of interest. E. coli is one of the main antimicrobials resistant pathogens in healthcare-associated infections reported to the National Healthcare Safety Network, being the primary cause of widespread pathologies such as significant diarrheal and extra-intestinal diseases or urinary tract infections. Furthermore, E. coli can be found as a bacterial food contamination and causes avian coli-bacillosis, one of the major bacterial diseases in the poultry industry and the most common avian disease communicable to humans. Currently, bacterium presence detection involve long time culture processes only to obtain a valid sample which could be properly detected. DEP concentration is a strong selective manipulation method which allows reducing sample preparation time. Moreover, by taking profit of IS, E. coli could be rapidly detected in the same equipment. For that reason, it is thought the proposed devices will be a useful tool for some current microbiology laboratories. Hence the mainly aims of the present thesis are: (I) to prove the feasibility of custom DEP generator for controlling bacteria and find the best signal to accomplish this, (II) to look for the best microfluidic chip option for bacteria preconcentration purposes on bioanalytical applications, (III) to test the feasibility of a custom IS device and (IV) to use the previous studies to design a complete electronic equipment, taken profit of combination of both techniques to have an autonomous system (V) To demonstrate the proof of concept of the full device with the real case of E. coli concentration.
El objetivo de esta tesis es el diseño de una instrumentación capaz de manipular y caracterizar células, a fin de realizar análisis más exhaustivos de elementos biológicos y acelerar procesos de detección de patógenos para aplicaciones de diagnóstico o de control de calidad de alimentos. El dispositivo se centra en dos tipos de técnicas eléctricas para la manipulación y detección de células: La dielectroforesis (DEP) y la medición de la bioimpedancia. La DEP permite manipular material biológico por medio de campos eléctricos, aprovechando las propiedades eléctricas de la célula y el medio en que se encuentra. La manipulación es por tanto ajustable, mediante el control de estas propiedades, así como a través de la geometría de los electrodos usados, la frecuencia y el módulo de la tensión aplicada. Por otro lado, la IS permite caracterizar material biológico mediante su comportamiento eléctrico en frecuencia. La medida se realiza a través de la aplicación de una corriente alterna controlada y la monitorización del efecto sobre el tejido mediante potencial eléctrico. Los dispositivos de IS son fácilmente integrables con técnicas dielectroforéticas de manipulación, fusionando manipulación con detección. En esta tesis, la combinación de estas técnicas permite la concentración de pequeños patógenos en grandes volúmenes de muestras y su posterior detección. Para ello, se crean diversos módulos de instrumentación electrónica. Algunos, están dedicados a generar señales alternas desfasadas a frecuencias óptimas para la manipulación de patógenos (módulo DEP). Otros, combinan módulos de generación, lectura y tratamiento digital, para la monitorización del comportamiento eléctrico de células (IS). Los módulos diseñados son validados en un entorno real controlado para concentrar y detectar la bacteria Escherichia Coli en grandes volúmenes de agua. Como resultado, se obtiene una electrónica modular válida, autónoma, portátil y de bajo coste, capaz de disminuir tiempos de preparación y detección de muestras en laboratorio.

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Marbach, Nathalie. „Risques lies a la manipulation du virus de la vaccine dans les laboratoires“. Université Louis Pasteur (Strasbourg) (1971-2008), 1992. http://www.theses.fr/1992STR1M099.

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Burn, C. C. „Effects of husbandry manipulations and the laboratory environment on rat health and welfare“. Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433382.

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Rabaud, David. „Manipulation et interaction de micro-bulles sous champ acoustique“. Phd thesis, Grenoble, 2010. http://tel.archives-ouvertes.fr/tel-00536932.

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Cette thèse traite de l'action des ondes acoustiques sur des bulles micrométriques excitées à leur fréquence de résonance. En effet, les bulles peuvent être considérées comme des oscillateurs mécaniques et les forces acoustiques à leur résonance, appelées forces de Bjerknes, ont des propriétés non triviales. De plus, les bulles interagissent entre elles par l'émission d'un champ secondaire, menant à leur auto-organisation sur un motif périodique. Ici, les bulles sont confinées dans des microcanaux, elles ne sont pas sphériques et frottent fortement sur les parois. L'étude expérimentale des forces acoustiques (primaire et secondaire) est alors précédée par la modélisation de l'écoulement des bulles, liant la friction sur les parois aux forces externes appliquées. Plusieurs applications aux laboratoires sur puce sont développées, dont le tri en taille, la division asymétrique contrôlée, l'aiguillage automatique à une bifurcation, et la manipulation de goutte.

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Jurníček, Jakub. „Návrh a realizace laboratorní úlohy řízení elektro-pneumatického manipulátoru FESTO“. Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2013. http://www.nusl.cz/ntk/nusl-230572.

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Presented thesis deals with pneumatic actuators, PLC control systems and physical properties of compressed air. The thesis introducing short view of pneumatic components of FESTO manipulator and LabVIEW programming. Experimental results are presented on three axis FESTO manipultor programmed by NI LabVIEW.

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Gajzler, Jakub. „Mikrovlnné výkonové zesilovače s vysokou účinností - laboratorní úloha“. Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217535.

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This Diploma thesis deals with methods that increase efficiency of transistor amplifiers. In the first part of the thesis we describe theoretical background of the constructions of power amplifiers. At first we deal with the classical method that is concerned with a change of position of the static operating point. Secondly we cover the multi harmonic manipulation method (MHM). This method is concerned with a proper loading of particular harmonic components and consequential shaping of voltage runs and currents on the collector. In the second part of the work we have constructed the substitutes of particular accesses. Constructed classes are AB, F and FMHM. Basic S parameters and output signal spectrum were measured. We can see from the calculated efficiencies AB = 11%, F = 16% a Fmhm = 18%, big increase of efficiency F and FMHM only by changing output network.

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Fong, Terrence W. „Design and testing of a Stewart Platform Augmented Manipulator for space applications“. Thesis, Massachusetts Institute of Technology, 1990. http://hdl.handle.net/1721.1/43002.

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Čepička, Martin. „Návrh konstrukce a řízení manipulátoru pro laboratorní měření teplotního pole“. Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2008. http://www.nusl.cz/ntk/nusl-228277.

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Diploma thesis is focused on design of construction of a simple manipulator. It will be used for laboratory measurement of temperature field. The goal is to design model of the manipulator, build up control software and verify by simulation. In thesis is described design of kinematical model, which was programmed in Matlab. Design of model was created in Autodesk Inventor Professional, which allows to conduct dynamic and compactness analysis of manipulator. The Autodesk Inventor Professional was also used for creating the productive documentation. Control of manipulator was design in program LabVIEW. This module will serve as input module to complex of measuring work.

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Bücher zum Thema "Laboratory manipulation"

1951-, Robertson Dominique, Hrsg. Manipulation and expression of recombinant DNA: A laboratory manual. 2. Aufl. Burlington, MA: Elsevier Academic, 2006.

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Robertson, Dominique. Manipulation and expression of recombinant DNA: A laboratory manual. San Diego: Academic Press, 1997.

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1918-, Shugar Gershon J., Ballinger Clinton T und Shugar Gershon J. 1918-, Hrsg. Chemical technicians' ready reference handbook. 5. Aufl. New York: McGraw-Hill, 2011.

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A, Fuchs James, und Messing Joachim W, Hrsg. An introduction to recombinant DNA techniques: Basic experiments in gene manipulation. 2. Aufl. Menlo Park, Calif: Benjamin/Cummings, 1988.

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1952-, Costantini Frank, und Lacy Elizabeth, Hrsg. Manipulating the mouse embryo: A laboratory manual. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory, 1986.

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Hogan, Brigid. Manipulating the mouse embryo: A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1986.

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Pomilio, Alicia B. Métodos experimentales de laboratorio en química orgánica. Washington, D.C: Secretaría General de la Organización de los Estados Americanos, Programa Regional de Desarrollo Científico y Tecnológico, 1988.

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Ralf, Kühn, Hrsg. Laboratory protocols for conditional gene targeting. Oxford: Oxford Uninversity Press, 1997.

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Bernier, Stéphane. 100 fiches pratiques: Sécurité des produits chimiques au laboratoire. 2. Aufl. Paris: Dunod, 2008.

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Sambrook, Joseph. Molecular cloning: A laboratory manual. 2. Aufl. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory, 1989.

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Buchteile zum Thema "Laboratory manipulation"

Li, Xiu-Qing. „Laboratory Methods for Investigating Nuclear and Cytoplasmic Genomes and Transcriptome“. In Somatic Genome Manipulation, 323–52. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2389-2_14.

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Gardner, R. L. „Embryo Transfer and Manipulation“. In New Developments in Biosciences: Their Implications for Laboratory Animal Science, 147–62. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-3281-4_27.

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Highfield, P. E. „The use of gene manipulation for the production of antigens“. In New Technologies in Clinical Laboratory Science, 45–48. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-4928-7_6.

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Teodoro, Vitor Duarte. „Direct Manipulation of Physical Concepts in a Computerized Exploratory Laboratory“. In Computer-Based Learning Environments and Problem Solving, 445–64. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-77228-3_21.

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Neuwelt, Edward A. „Blood-Brain Barrier Manipulation: Current Status of Laboratory and Clinical Studies“. In New Concepts of a Blood—Brain Barrier, 277–85. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4899-1054-7_27.

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D’Amelio, Marcello, und Francesco Cecconi. „Apoptosome Pharmacological Manipulation: From Current Developments in the Laboratory to Clinical Implications“. In Apoptosome, 271–81. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3415-1_14.

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Milton, John, und Toru Ohira. „Characterizing and Manipulating Oscillations“. In Mathematics as a Laboratory Tool, 339–62. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-69579-8_12.

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Schäfer, Martin, und Stefan Meldau. „Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies“. In Methods in Molecular Biology, 127–39. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6831-2_10.

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Luo, Yiqi, und Dafeng Hui. „Gradual Global Environmental Change in the Real World and Step Manipulative Experiments in Laboratory and Field: The Necessity of Inverse Analysis“. In Real World Ecology, 267–91. New York, NY: Springer New York, 2009. http://dx.doi.org/10.1007/978-0-387-77942-3_10.

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Foschi, Martha. „Hypotheses, Operationalizations, and Manipulation Checks“. In Laboratory Experiments in the Social Sciences, 247–68. Elsevier, 2014. http://dx.doi.org/10.1016/b978-0-12-404681-8.00011-x.

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Konferenzberichte zum Thema "Laboratory manipulation"

Kardos, Slavomir, Alena Pietrikova, Juraj Durisin und Martin Kusko. „Encased manipulation chamber for technologic laboratory“. In 2010 33rd International Spring Seminar on Electronics Technology (ISSE). IEEE, 2010. http://dx.doi.org/10.1109/isse.2010.5547338.

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Tzafestas, C. S., N. Palaiologou und M. Alifragis. „Experimental Evaluation and Pilot Assessment Study of a Virtual and Remote Laboratory on Robotic Manipulation“. In Proceedings of the IEEE International Symposium on Industrial Electronics. IEEE, 2005. http://dx.doi.org/10.1109/isie.2005.1529184.

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Nagchaudhuri, Abhijit, Shinivas Saishyam, John Wood und Anthony Stockus. „Mechatronics Laboratory at UMES: A Platform to Promote Synergy in Education and Research Across Disciplinary Boundaries“. In ASME 2003 International Mechanical Engineering Congress and Exposition. ASMEDC, 2003. http://dx.doi.org/10.1115/imece2003-42883.

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Mechatronics is the synergistic integration of mechanics, instrumentation and control, software engineering and information technology. As such it integrates well with not only the modern evolution of mechanical engineering curricula but has wide and growing manifestation in the new generation of industrial products as well as children’s toys. The present set-up of the laboratory consists of an industrial SCARA (Selective Compliance Articulated Robot Arm) robot equipped with machine vision capability for guidance, inspection and recognition associated with robotic manipulation of parts. An open loop stable vibration control platform, an open loop unstable inverted pendulum and a dual water tank system interfaced with appropriate sensors and actuators provide capabilities for learning both analog and digital control of systems belonging to the solid mechanics and fluid mechanics fields. Modern software tools that include graphical programming capability using Simulink and compilation via Real time Windows Target, Real time Workshop (all from Mathworks) and Visual C++ (Microsoft) allow for developing and executing variety of control algorithms on these systems. Capabilities for remote operation of these systems over the internet have also been implemented. The laboratory facilities provide education and research capability at the interfaces of traditional disciplinary boundaries. The laboratory is also equipped with LEGO MINDSTORM and LEGO DACTA products as well as the MIT Handyboard for exploration of mechatronics and robotics activities for prospective engineers and K-12 students.

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Alazzam, Anas, Bashar El-Khasawneh und Mohammad Abutayeh. „Simple, Fast, and Low Cost Fabrication Methods of Microchannels for Manipulation of Living Cells“. In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-66152.

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This work details simple non-cleanroom fabrication techniques to build hermetic microchannels using laboratory available material and equipment. Four different methods are presented for fast fabrication of microchannels at low cost. The microchannels are to be used for the manipulation of living cells. These methods of fabrication of microfluidics devices have previously been used and documented as reactors, fluid mixer, and for fluids transportation and inspection. In this work, all methods were used for manipulation of living cells. Each method, its advantages and disadvantages for this particular application are reported. The microfluidic device built using the first method includes a polymer-based part and a glass substrate with a layer of patterned electrodes. The Polymer-based microchannel is made outside the cleanroom facility using a simple mold made from adhesive tape. Moreover, the fabrication of a non-polymer microchannel made from double-sided tape is described. The microchannel height is about 50 μm while the width varies between 100 μm to a few hundred microns. The third method of fabrication is made by a 3D printer. The master molds for the polymer-based microfluidic device are fabricated by 3D printing of biocompatible material on glass substrate. The fourth method is a simple embossing of a male die in plastic or polymer substrate. A computer numerical control (CNC) machine was used to fabricate the embossing mold in stainless steel, brass, and aluminum. Microchannels were created by stamping the mold in a Cyclic Olefin Copolymer (COC) substrate.

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Stewart, Paul, Pietro Buttolo und Yifan Chen. „CAD Data Representations for Haptic Virtual Prototyping“. In ASME 1997 Design Engineering Technical Conferences. American Society of Mechanical Engineers, 1997. http://dx.doi.org/10.1115/detc97/cie-4307.

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Abstract For many years designers have relied on visual feedback and two-dimensional pointing devices to interact with Computer-Aided Design (CAD) models. The “sense of touch”, that is fundamental to interaction and manipulation in everyday life, is not yet a part of the standard human-computer interface. An on-going project at the Ford Research Laboratory has created an immersive virtual prototyping system with which a designer can not only visualize a CAD model but also “touch” it using a force-feedback device. This paper will briefly introduce human perception of haptic information and then describe the process of haptically rendering an object. Then the issues involved with the correct selection of a data representation for haptic rendering will be addressed. Lastly, a brief description of the experimental system implemented at the Ford Research Laboratory will be given.

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Ghafar-Zadeh, Ebrahim, Mohamad Sawan und Daniel Therriault. „Direct-Write Fabrication of Microchannel in Epoxy Matrix“. In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-82905.

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This paper describes the fabrication of microchannels by direct-write assembly and their integration into CMOS circuit technologies. A robotic apparatus was used to deposit a fugitive ink filament on glass and CMOS chip substrates. An epoxy and a nanocomposite matrix were both used to enclose the ink which was subsequently removed under heat and pressure in order to form a microchannel. Here, we demonstrate the fabrication of a microchannel by direct-write assembly on top of a CMOS chip for potential laboratory-on-chip applications such as bioparticle detection and manipulation.

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Langerman, Michael A., Gregory A. Buck, Umesh A. Korde und Vojislav D. Kalanovic. „Thermal Control of Laser Powder Deposition: Heat Transfer Considerations“. In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-60386.

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Laser based solid free-form fabrication is an emerging metallurgical forming process aimed at rapid production of high quality, near net shape products directly from starting powders. Laser powder deposition shares, with other free-form technologies, the common characteristic that part fabrication occurs directly from a 3-D computer aided design (CAD) model. The microstructure evolution and resulting material properties of the component part (strength, ductility, etc.) fabricated using laser deposition are dependent upon process operating parameters such as melt pool size, laser power, head (manipulator) speed, and powder flow rate. Presently, set points for these parameters are often determined through manual manipulation of the system control and trial and error. This paper discusses the development of a path-planning, feed-forward, process-driven control system algorithm that generates a component part thermal history within given constraints, thereby assuring optimal part quality and minimizing final residual stresses. A thermal model of the deposition process drives the control algorithm. The development of the thermal model is the subject of this paper. The model accounts for temperature-dependent properties and phase change processes. Model validation studies are presented including comparisons with known analytic solutions as well as comparisons with data from experiments conducted in the laser laboratory at SDSM&T.

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Zhou, Nana, Chen Yang und David Tucker. „Evaluation of Compressor Bleed Air Transients in a Fuel Cell Gas Turbine Hybrid System Using Hardware Simulation“. In ASME Turbo Expo 2015: Turbine Technical Conference and Exposition. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/gt2015-43596.

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Thermal management in the fuel cell component was a critical issue in the operation of a solid oxide fuel cell gas turbine (SOFC/GT) hybrid system. The effective management of fuel cell cathode air mass flow was thought to be a potential method to improve the thermal management during transients. The U.S. Department of Energy, National Energy Technology Laboratory (NETL) designed and built a hybrid performance (HyPer) facility by interfacing a real time solid oxide fuel cell system numerical model through hardware with a physical gas turbine system. Perturbations were accomplished by diverting part of the compressor discharge directly to atmosphere through the manipulation of a bleed-air bypass valve in open loop experiments using the HyPer facility. Two tests were performed: the fuel cell numerical simulation model was both decoupled and fully coupled with the gas turbine hardware component. The responses of both physical subsystem and virtual subsystem to the disturbances were evaluated in this paper. Distributed temperatures and current densities along the fuel cell were evaluated. Turbine speed and system pressures were analyzed. The application of bleed-air bypass valve was shown to have a minimal impact on cathode airflow, but a significant effect on turbine speed. Thus, the manipulation of compressor bleed was expected to be an effective means to mitigate the impact of a sudden increase in turbine speed, such as fuel cell load reductions or load trips.

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Dubey, Venketesh N., und Richard M. Crowder. „Grasping and Control Issues in Adaptive End Effectors“. In ASME 2004 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2004. http://dx.doi.org/10.1115/detc2004-57126.

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Research into robotic grasping and manipulation has led to the development of a large number of tendon based end effectors. Many are, however, developed as a research tool, which are limited in application to the laboratory environment. The main reason being that the designs requiring a large number of actuators to be controlled. Due to the space and safety requirements, very few have been developed and commissioned for industrial applications. This paper presents design of a rigid link finger operated by a minimum number of actuators, which may be suitable for a number of adaptive end effectors. The adaptive nature built into the end effector (due to limited number of actuators) presents considerable problems in grasping and control. The paper discusses the issues associated with such designs. The research can be applicable to any adaptive end effectors that are controlled by limited number of actuators and evaluates their suitability in industrial environment.

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Tucker, David, Larry Lawson, Thomas P. Smith und Comas Haynes. „Evaluation of Cathodic Air Flow Transients in a Hybrid System Using Hardware Simulation“. In ASME 2006 4th International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2006. http://dx.doi.org/10.1115/fuelcell2006-97107.

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Effective control of cathode airflow in a direct fired solid oxide fuel cell gas turbine (SOFC/GT) hybrid power system is critical to thermal management of a fuel cell stack. Hybrid fuel cell turbine designs often incorporate the use of a valved hot air bypass in parallel with the cathode flow to divert a portion of the compressor effluent around the fuel cell system. The primary objective of this valve in the early development of hybrid power systems was to facilitate system startup. From a system controls perspective, the hot air bypass offers the means to balance and manipulate the level of airflow supplied to the fuel cell stack at a minimal efficiency penalty. Manipulation of this valve has a significant impact on stack performance and reliability, as well as cathodic exhaust airflow conditions. Since the turbine is directly coupled to the fuel cell subsystem through the cathode airflow, non-linear effects are propagated through the system components in response to any hot air bypass valve change. The effect of cathode flow transients on hybrid system performance has been evaluated though the manipulation of a hot air bypass valve on a hardware-based simulation facility designed and built by the U.S. Department of Energy, National Energy Technology Laboratory (NETL). A brief overview of this experimental facility is provided and has been described in more detail previously. Open loop experiments were conducted using the facility, where a perturbation was made to the hot air bypass flow and turbine speed was allowed to change in response. The impact of the transients to both fuel cell and turbine performance are discussed.

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Berichte der Organisationen zum Thema "Laboratory manipulation"

Kress, R. L., J. F. Jansen, L. J. Love und A. M. H. Basher. Hydraulic manipulator design, analysis, and control at Oak Ridge National Laboratory. Office of Scientific and Technical Information (OSTI), September 1996. http://dx.doi.org/10.2172/665942.

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Matthew, Gray. Data from "Winter is Coming – Temperature Affects Immune Defenses and Susceptibility to Batrachochytrium salamandrivorans". University of Tennessee, Knoxville Libraries, Januar 2021. http://dx.doi.org/10.7290/t7sallfxxe.

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Environmental temperature is a key factor driving various biological processes, including immune defenses and host-pathogen interactions. Here, we evaluated the effects of environmental temperature on the pathogenicity of the emerging fungus, Batrachochytrium salamandrivorans (Bsal), using controlled laboratory experiments, and measured components of host immune defense to identify regulating mechanisms. We found that adult and juvenile Notophthalmus viridescens died faster due to Bsal chytridiomycosis at 14 ºC than at 6 and 22 ºC. Pathogen replication rates, total available proteins on the skin, and microbiome composition likely drove these relationships. Temperature-dependent skin microbiome composition in our laboratory experiments matched seasonal trends in wild N. viridescens, adding validity to these results. We also found that hydrophobic peptide production after two months post-exposure to Bsal was reduced in infected animals compared to controls, perhaps due to peptide release earlier in infection or impaired granular gland function in diseased animals. Using our temperature-dependent infection results, we performed a geographic analysis that suggested that N. viridescens populations in the northeastern United States and southeastern Canada are at greatest risk for Bsal invasion. Our results indicate that environmental temperature will play a key role in the epidemiology of Bsal and provide evidence that temperature manipulations may be a viable Bsal management strategy.

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March-Leuba, S., J. F. Jansen, R. L. Kress, S. M. Babcock und R. V. Dubey. Development of the Symbolic Manipulator Laboratory modeling package for the kinematic design and optimization of the Future Armor Rearm System robot. Office of Scientific and Technical Information (OSTI), August 1992. http://dx.doi.org/10.2172/6956182.

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March-Leuba, S., J. F. Jansen, R. L. Kress, S. M. Babcock und R. V. Dubey. Development of the Symbolic Manipulator Laboratory modeling package for the kinematic design and optimization of the Future Armor Rearm System robot. Ammunition Logistics Program. Office of Scientific and Technical Information (OSTI), August 1992. http://dx.doi.org/10.2172/10191974.

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