Zeitschriftenartikel: „Klebsiella pneumoniae Strain PB12“

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Mandal, Amit K., Ipsita K. Sen, Prasenjit Maity, Sourav Chattopadhyay, Ranadhir Chakraborty, Somenath Roy und Syed S. Islam. „Structural elucidation and biological studies of a novel exopolysaccaride from Klebsiella pneumoniae PB12“. International Journal of Biological Macromolecules 79 (August 2015): 413–22. http://dx.doi.org/10.1016/j.ijbiomac.2015.04.077.

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Yan, Kai, Changfu Li, Weiyu Wang, Juan Guo und Haifeng Wang. „The Molecular Identification and Comprehensive Analysis of Klebsiella pneumoniae Isolated from Industrial Wastewater“. Separations 11, Nr. 4 (17.04.2024): 121. http://dx.doi.org/10.3390/separations11040121.

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Industrial wastewater typically contains many organic and inorganic pollutants and is also contaminated by various microorganisms. Microbial species in industrial wastewater have not been extensively investigated. In this experiment, a Klebsiella pneumoniae strain was isolated for the first time from industrial wastewater containing a high concentration of sulfate and phosphate. Mass spectrometry, genetic analysis, and biochemical identification were conducted to understand the genetic and biochemical characteristics of this Klebsiella pneumoniae strain recovered from industrial wastewater. Growth experiments revealed that it exhibited an excellent growth rate in nutrient broth. Further analyses showed that the strain was sensitive to most antibiotics but resistant to chloramphenicol and nitrofurantoin. It also exhibited significant resistance to piperacillin/tazobactam and cefotaxime/clavulanic acid. Resistance gene experiments indicated the presence of gyrA, OqxB, and ParC genes associated with antibiotic resistance in the isolated Klebsiella pneumoniae strain. Proteomics uncovered the following three proteins related to drug resistance: the multi-drug resistant outer membrane protein MdtQ, the multi-drug resistant secretion protein, and the modulator of drug activity B, which are coexistent in Klebsiella pneumoniae. Proteomics and bioinformatics analyses further analyzed the protein composition and functional enrichment of Klebsiella pneumoniae. The isolation of Klebsiella pneumoniae from a high concentration in sulfate and phosphate industrial wastewater provides a new direction for further research on the characteristics and drug resistance traits of industrial wastewater microorganisms and the potential risks they may pose when released into the environment.

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Aminah, Aminah, Citra Trisna und Dewi Lokida. „Deteksi Molekuler Klebsiella pneumoniae K1, K2, dan K5 yang Diisolasi dari Berbagai Spesimen Klinis“. Journal of Medical Laboratory Research 2, Nr. 1 (09.12.2023): 1–6. http://dx.doi.org/10.36743/jomlr.v2i1.633.

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Klebsiella pneumoniae adalah salah satu penyebab utama healthcare-associated infections dengan tingkat multidrug resistance (MDR) tinggi, terutama klon penghasil extended-spectrum beta-lactamase (ESBL) dan carbapenemase (CP). Serotipe kapsul Klebsiella pneumoniae sangat bervariasi sesuai tingkat resistensinya terhadap serum dengan K1, K2, dan K5 menjadi penanda strain hipervirulen yang berbeda dari Klebsiella pneumoniae klasik dalam masyarakat pada umumnya. Penelitian ini bertujuan untuk mengaplikasikan teknik polymerase chain reaction (PCR) untuk mendeteksi genotipe kapsul K1, K2, dan K5 Klebsiella pneumoniae asal pasien Rumah Sakit Umum (RSU) Kabupaten Tangerang. Isolasi dan uji resistensi dilakukan oleh Laboratorium RSU Kabupaten Tangerang. Konfirmasi spesies K. pneumoniae dan deteksi genotipe kapsul dengan metode PCR dilakukan di Laboratorium Molekuler Poltekkes Kemenkes Banten. Sebanyak delapan isolat (26,7%) merupakan K. pneumoniae resistan Carbapenem dan 11 (36,7%) penghasil extended spectrum beta-lactamase (ESBL). Dari total 31 sampel yang diperoleh, hanya satu isolat yang tidak dapat dikonfirmasi. Seluruh isolat yang terkonfirmasi K. pneumoniae diproses untuk tahap selanjutnya. Pemeriksaan PCR menggunakan primer untuk mendeteksi genotipe kapsul K1, K2, dan K5 menunjukkan hanya satu isolat (3,3%) yang diduga merupakan strain hipervirulen dengan genotipe kapsul K2. Tidak ada genotipe K1 dan K5 pada semua isolat yang diperiksa.

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Padilla, Emma, Diana Alonso, Antonio Doménech-Sánchez, Cristina Gomez, José Luis Pérez, Sebastián Albertí und Nuria Borrell. „Effect of Porins and Plasmid-Mediated AmpC β-Lactamases on the Efficacy of β-Lactams in Rat Pneumonia Caused by Klebsiella pneumoniae“. Antimicrobial Agents and Chemotherapy 50, Nr. 6 (Juni 2006): 2258–60. http://dx.doi.org/10.1128/aac.01513-05.

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ABSTRACT The in vivo activities of imipenem, meropenem, and cefepime were studied in a model of rat pneumonia caused by a plasmid-mediated AmpC β-lactamase ACT-1-producing Klebsiella pneumoniae strain (K. pneumoniae strain 12) and a derivative porin-deficient mutant (K. pneumoniae strain 12dp). No differences between these activities were seen with K. pneumoniae 12. Only meropenem showed an activity slightly better than that of imipenem with K. pneumoniae 12dp.

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Crespo-Yanez, Xenia, und Imen Ayadi. „The AI516 and AI523 antibodies recognize the Klebsiella pneumoniae KpGe strain by flow cytometry“. Antibody Reports 5, Nr. 1 (03.03.2022): e682. http://dx.doi.org/10.24450/journals/abrep.2022.e682.

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The recombinant antibodies AE516 and AI523 bind to the surface of the Klebsiella pneumoniae KpGe strain, as detected by flow cytometry. They do not bind to a K. pneumoniae strain defective in O-antigen synthesis.

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Martins, Paula Fabiane, Camila Ortiz Martinez, Giselle de Carvalho, Paulo Irajara Borba Carneiro, Ricardo Antunes Azevedo, Sônia Alvim Veiga Pileggi, Itamar Soares de Melo und Marcos Pileggi. „Selection of microorganisms degrading S-Metolachlor herbicide“. Brazilian Archives of Biology and Technology 50, Nr. 1 (Januar 2007): 153–59. http://dx.doi.org/10.1590/s1516-89132007000100019.

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The aim of this work was to study herbicide degradation through selected microorganisms from humus and soil subjected to different plantation systems. The following bacterial species were identified: Klebsiella pneumoniae pneumoniae GC s.B strain 1, Pseudomonas alcaligenes, Enterobacter aerogenes GC s.A and Klebsiella pneumoniae pneumoniae GC s.B strain 2. Growth studies yet suggested the possibility of a very long lag phase. Although, culture with the herbicide presented biofilm formation and there were color changes in the herbicide that could have interfered with the espectrophotometry readings. After 5 days of incubation at 35ºC, the difference in the concentration of herbicide was 14.42% on average and after 10 days, 35.01%.

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Chen, Kong, Meagan Goodwin, Jeremy McAleer, Nikki Nguyen, Emily Way und Jay Kolls. „Recombinant outer membrane protein: a potential candidate for Th17 based vaccine against Klebsiella pneumoniae. (VAC7P.967)“. Journal of Immunology 192, Nr. 1_Supplement (01.05.2014): 141.12. http://dx.doi.org/10.4049/jimmunol.192.supp.141.12.

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Abstract Bacterial pneumonia is a leading cause of mortality and one major pathogen associated with this disease is Klebsiella pneumoniae. Recently, the emergence of antibiotic resistant strains demands an effective vaccine against these bacteria. We have previously shown that intranasal immunization with heat-killed K. pneumoniae or crude outer membrane proteins isolated from K. pneumoniae induced antigen specific Th17 responses and these Th17 cells conferred serotype independent protection against various clinical isolates of K. pneumoniae including the recently described multidrug resistant New Delhi Metallo-beta-lactamase-1 strain. To develop a clinically relevant Klebsiella vaccine, we cloned single OMP genes by PCR from K. pneumoniae and successfully expressed one recombinant outer membrane protein, OmpX, in the BL21 E. coli strain. The purified recombinant OmpX was recognized by Klebsiella immune serum by direct ELISA and also recognized by Th17 cells from Klebsiella immunized mice. In vivo, intranasal immunization of the purified OmpX induced robust mucosal Th17 responses and left IL-17 producing gamma-delta T cells unaffected. Ongoing work will determine whether these Th17 responses will result in serotype independent protection against live bacterial challenge including the multidrug resistant strains.

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Kang, Fuqiang, Zili Chai, Beiping Li, Mingda Hu, Zilong Yang, Xia Wang, Wenting Liu, Hongguang Ren, Yuan Jin und Junjie Yue. „Characterization and Diversity of Klebsiella pneumoniae Prophages“. International Journal of Molecular Sciences 24, Nr. 11 (23.05.2023): 9116. http://dx.doi.org/10.3390/ijms24119116.

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Klebsiella pneumoniae is a common human commensal and opportunistic pathogen. In recent years, the clinical isolation and resistance rates of K. pneumoniae have shown a yearly increase, leading to a special interest in mobile genetic elements. Prophages are a representative class of mobile genetic elements that can carry host-friendly genes, transfer horizontally between strains, and coevolve with the host’s genome. In this study, we identified 15,946 prophages from the genomes of 1437 fully assembled K. pneumoniae deposited in the NCBI database, with 9755 prophages on chromosomes and 6191 prophages on plasmids. We found prophages to be notably diverse and widely disseminated in the K. pneumoniae genomes. The K. pneumoniae prophages encoded multiple putative virulence factors and antibiotic resistance genes. The comparison of strain types with prophage types suggests that the two may be related. The differences in GC content between the same type of prophages and the genomic region in which they were located indicates the alien properties of the prophages. The overall distribution of GC content suggests that prophages integrated on chromosomes and plasmids may have different evolutionary characteristics. These results suggest a high prevalence of prophages in the K. pneumoniae genome and highlight the effect of prophages on strain characterization.

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Brisse, Sylvain, Virginie Passet und Patrick A. D. Grimont. „Description of Klebsiella quasipneumoniae sp. nov., isolated from human infections, with two subspecies, Klebsiella quasipneumoniae subsp. quasipneumoniae subsp. nov. and Klebsiella quasipneumoniae subsp. similipneumoniae subsp. nov., and demonstration that Klebsiella singaporensis is a junior heterotypic synonym of Klebsiella variicola“. International Journal of Systematic and Evolutionary Microbiology 64, Pt_9 (01.09.2014): 3146–52. http://dx.doi.org/10.1099/ijs.0.062737-0.

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Strains previously classified as members of Klebsiella pneumoniae phylogroups KpI, KpII-A, KpII-B and KpIII were characterized by 16S rRNA (rrs) gene sequencing, multilocus sequence analysis based on rpoB, fusA, gapA, gyrA and leuS genes, average nucleotide identity and biochemical characteristics. Phylogenetic analysis demonstrated that KpI and KpIII corresponded to K. pneumoniae and Klebsiella variicola , respectively, whereas KpII-A and KpII-B formed two well-demarcated sequence clusters distinct from other members of the genus Klebsiella . Average nucleotide identity between KpII-A and KpII-B was 96.4 %, whereas values lower than 94 % were obtained for both groups when compared with K. pneumoniae and K. variicola . Biochemical properties differentiated KpII-A, KpII-B, K. pneumoniae and K. variicola , with acid production from adonitol and l-sorbose and ability to use 3-phenylproprionate, 5-keto-d-gluconate and tricarballylic acid as sole carbon sources being particularly useful. Based on their genetic and phenotypic characteristics, we propose the names Klebsiella quasipneumoniae subsp. quasipneumoniae subsp. nov. and K. quasipneumoniae subsp. similipneumoniae subsp. nov. for strains of KpII-A and KpII-B, respectively. The type strain of K. quasipneumoniae sp. nov. and of K. quasipneumoniae subsp. quasipneumoniae subsp. nov. is 01A030T ( = SB11T = CIP 110771T = DSM 28211T). The type strain of K. quasipneumoniae subsp. similipneumoniae subsp. nov. is 07A044T ( = SB30T = CIP 110770T = DSM 28212T). Both strains were isolated from human blood cultures. This work also showed that Klebsiella singaporensis is a junior heterotypic synonym of K. variicola .

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Usman, Nazeef Idris. „Phenotypic characterization, detection of virulence factors, and antibacterial susceptibility profile of clinical isolates of Klebsiella pneumoniae“. Gadau Journal of Pure and Allied Sciences 1, Nr. 2 (02.10.2022): 121–32. http://dx.doi.org/10.54117/gjpas.v1i2.11.

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Klebsiella pneumoniae has been one of the most occurring bacteria in clinical samples causing community-acquired and nosocomial infections. It has been developing resistance to most antimicrobial agents. These have increased the pathogenicity and the chance for the evolvement of more invasive K. pneumoniae. The study was aimed at the identification and detection of Klebsiella pneumoniae from clinical samples. Some selected virulence factors were detected and the antibacterial susceptibility profile of the isolates was conducted. The incubation period, infective dose, and mortality rate of K.pneumoniae were measured using Baggs albino rats (BALB/c) strain. The isolation and identification of 56 Klebsiella pneumoniae from 223 clinical samples were made. Out of 56 K. pneumoniae isolates, all manifested mucoid phenotype, 44(78.6%) capsule antigen and 13(23.2%) Siderophore. The antimicrobial test conducted identified Zinacef 51(91.1%), Rocephin 50 (89.3%), and Ampiclox 50 (91.1%) as the most resistant. Ciprofloxacin 49 (87.5%), chloramphenicol 35(62.5%), Gentamycin 32(57.1%), and Amoxicillin 28 (50%) were the most susceptible to Klebsiella pneumoniae. The mouse lethality test shows that K. pneumoniae hypermucoviscous strain can cause 41.7% lethality and 66.7% mortality in Baggs Albino rats. Meanwhile an infection dose 105cfu/ml, 107cfu/ml and 109cfu/ml produced an incubation period of 9 days, 7days and 5days. The chi-square test shows no significant association between the isolate and the gender of the patients, but there is a significant association between the samples and isolates identified p <0.05. Likewise, the association between mucoid phenotype, capsule antigen, siderophore, and the type of infection is significant (p<0.05). It is finally concluded that K. pneumoniae is the second most prevailing bacterium causing community-acquired infection the resistant pattern recorded identified the test bacteria as a multidrug-resistant strain and manifestation of the virulent factor depends on the type and site of infection caused by the K. pneumoniae.

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Kitchel, Brandon, Daniel R. Sundin und Jean B. Patel. „Regional Dissemination of KPC-Producing Klebsiella pneumoniae“. Antimicrobial Agents and Chemotherapy 53, Nr. 10 (17.08.2009): 4511–13. http://dx.doi.org/10.1128/aac.00784-09.

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ABSTRACT Production of a Klebsiella pneumoniae carbapenemase (KPC) is the most common mechanism of carbapenem resistance in the United States; however, until now, KPC-producing isolates have not been found in western Michigan. Molecular typing of two KPC-producing K. pneumoniae isolates from Michigan showed their similarity to other Midwestern isolates. They were also unrelated to the dominant sequence type observed throughout the United States, multilocus sequence type 258. This could represent regional dissemination of another KPC-producing K. pneumoniae strain.

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Tofarides, Andreas G., Panagiotis Dimitriou, Georgios K. Nikolopoulos, Dimitrios Rogkas, Christina Flourou, Elina Khattab, Diamanto Kasapi, Chara Azina und Eirini Christaki. „Factors Associated with Extended-Spectrum β-Lactamases and Carbapenem-Resistant Klebsiella pneumoniae Bloodstream Infections: A Five-Year Retrospective Study“. Pathogens 12, Nr. 11 (25.10.2023): 1277. http://dx.doi.org/10.3390/pathogens12111277.

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Klebsiella pneumoniae is one of the leading causes of nosocomial infections. It has been estimated that nosocomial infection by Klebsiella pneumoniae comprises 3–8% of all nosocomial infections. Klebsiella pneumoniae bloodstream infections (BSIs) occur worldwide with varying mortality. Resistant strains, like those producing extended-spectrum beta-lactamases (ESBL) and carbapenemases, are becoming increasingly common, especially in hospital settings, posing therapeutic challenges. In this article, we aimed to study the epidemiology and risk factors of BSIs due to resistant Klebsiella pneumoniae strains in the period 1 January 2014–31 December 2018 at the Nicosia General Hospital, the largest tertiary hospital in Cyprus. Data on demographics, co-morbidities, prior hospitalization, prior intensive care unit (ICU) admission, previous antimicrobial use, nosocomial acquisition of the infection, the presence of a prosthetic device or surgery, and the primary site of infection were retrospectively recorded. Associations between the detection of ESBL Klebsiella pneumoniae BSIs and factors/covariates were examined using logistic regression. This study involved 175 patients with BSI caused by Klebsiella pneumoniae. Of these, 61 BSIs were caused by ESBL strains, 101 by non-ESBL, and 13 by carbapenem-resistant (CR) strains. In univariable analyses, age, sex, heart disease, antimicrobial use during current admission, previous hospitalization (ward or ICU), and primary BSI were associated with the presence of an ESBL strain. Antibiotic use during current admission and heart disease remained statistically significantly associated with ESBL Klebsiella pneumoniae BSI in multivariable models. Antibiotic use during current admission, respiratory infection, and a recent history of surgery were more prevalent among CR Klebsiella pneumoniae BSI patients than among non-CR Klebsiella pneumoniae BSI patients. Our study showed that recent antimicrobial use and heart disease were associated with BSI due to ESBL-producing Klebsiella pneumoniae. This finding could inform clinical practice in hospital settings.

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Gootz, Thomas D., Mary Kay Lescoe, Fadia Dib-Hajj, Brian A. Dougherty, Wen He, Phyllis Della-Latta und Richard C. Huard. „Genetic Organization of Transposase Regions Surrounding blaKPC Carbapenemase Genes on Plasmids from Klebsiella Strains Isolated in a New York City Hospital“. Antimicrobial Agents and Chemotherapy 53, Nr. 5 (02.03.2009): 1998–2004. http://dx.doi.org/10.1128/aac.01355-08.

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ABSTRACT Carbapenem-resistant Klebsiella strains carrying Klebsiella pneumoniae carbapenemases (KPC) are endemic to New York City and are spreading across the United States and internationally. Recent studies have indicated that the KPC structural gene is located on a 10-kb plasmid-borne element designated Tn4401. Fourteen Klebsiella pneumoniae strains and one Klebsiella oxytoca strain isolated at a New York City hospital in 2005 carrying either bla KPC-2 or bla KPC-3 were examined for isoforms of Tn4401. Ten of the Klebsiella strains contained a 100-bp deletion in Tn4401, corresponding to the Tn4401a isoform. The presence of this deletion adjacent to the upstream promoter region of bla KPC in Tn4401a resulted in a different −35 promoter sequence of TGGAGA than that of CTGATT present in isoform Tn4401b. Complete sequencing of one plasmid carrying bla KPC from each of three nonclonal isolates indicated the presence of genes encoding other types of antibiotic resistance determinants. The 70.6-kb plasmid from K. pneumoniae strain S9 carrying bla KPC-2 revealed two identical copies of Tn4401b inserted in an inverse fashion, but in this case, one of the elements disrupted a group II self-splicing intron. In K. pneumoniae strain S15, the Tn4401a element carrying bla KPC-2 was found on both a large 120-kb plasmid and a smaller 24-kb plasmid. Pulsed-field gel electrophoresis results indicate that the isolates studied represent a heterogeneous group composed of unrelated as well as closely related Klebsiella strains. Our results suggest that endemic KPC-positive Klebsiella strains constitute a generally nonclonal population comprised of various alleles of bla KPC on several distinct plasmid genetic backgrounds. This study increases our understanding of the genetic composition of the evolving and expanding role of KPC-producing, healthcare-associated, gram-negative pathogens.

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Cortés, Guadalupe, Beatriz de Astorza, Vicente J. Benedí und Sebastián Albertí. „Role of the htrA Gene in Klebsiella pneumoniae Virulence“. Infection and Immunity 70, Nr. 9 (September 2002): 4772–76. http://dx.doi.org/10.1128/iai.70.9.4772-4776.2002.

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ABSTRACT We recently described the use of mini-Tn5 to generate complement-sensitive mutants derived from a complement-resistant Klebsiella pneumoniae clinical isolate deficient in the lipopolysaccharide O side chain. One mutant with a reduced capacity to survive in nonimmune human sera carried the transposon inserted in the htrA gene. We cloned and sequenced the gene and predicted from the deduced amino acid sequence that the putative HtrA homolog contains structural features similar to those of previously described HtrA proteins. To investigate the biological functions and the role of the htrA gene in the virulence of K. pneumoniae, we constructed an isogenic mutant by insertion-duplication mutagenesis. Characterization of the mutant showed that it had greater sensitivity to temperature (50°C) and oxidative stress (H2O2) than the parent strain. Furthermore, the htrA mutant produced less capsule, bound more molecules of complement component C3, and was more sensitive to complement and whole-blood killing than was the parent strain. Finally, disruption of the htrA gene in a virulent K. pneumoniae strain caused a reduction of its virulence in a mice model. Our results indicate that the htrA gene plays an important role in the virulence of K. pneumoniae.

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Singh, Priyanka. „The outcome of different β lactamase production in Klebsiella pneumoniae subspecies pneumoniae isolated from different clinical specimen in tertiary care hospital: a study“. International Journal of Research in Medical Sciences 6, Nr. 5 (25.04.2018): 1568. http://dx.doi.org/10.18203/2320-6012.ijrms20181456.

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Background: Klebsiella pneumoniae subspecies pneumoniae is one of the most commonly isolated bacterial species in Microbiology laboratories and can cause Infection in hospitalised patients with strain resistant to many antimicrobials. Klebsiella pneumoniae is more frequently recovered from clinical specimen. The carrier rate as high as 20% may occur in hospitalized patient.Methods: Isolates are screened for presumptive ESBL by reduced susceptibility to CAC, CAZ, Carbapenemase by Classical Hodge test and modified Hodge test, MBL by DP Test, AmpC production by testing their susceptibility to Cefoxitin using Kirby-Bauer disk diffusion method. KPC by combined Disc method using PBA.Results: Out of total 300 Klebsiella pneumoniae strains studied, 98 (32.7%) were isolated from urine followed by pus and wound swab 74 (24.7%) and 63 (21%) were isolated from blood.Conclusions: Maximum (23.5%) ESBL, (10.2%) AmpC β- lactamases and (10.2%) KPC producing strains were isolated from urine sample. Maximum 32.1% MBL producing Klebsiella pneumoniae strains were isolated from pus and wound swab.

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Wu, June Hsieh, und Cheng Gie Tsai. „Infectivity of Hepatic Strain Klebsiella pneumoniae in Diabetic Mice“. Experimental Biology and Medicine 230, Nr. 10 (November 2005): 757–61. http://dx.doi.org/10.1177/153537020523001009.

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Besides urinary tract infection (UTI) and pneumonia, increased severe liver abscesses caused by Klebsiella pneumoniae (KP), especially in diabetic patients, have been observed in infections acquired in hospitals. This indicates that different KP strains with higher virulence have emerged in recent years. Our goal was to investigate the infectivity of KP isolates in mice from liver abscess or UTI patients. Mice were injected with streptozotocin to induce diabetes. Male ICR mice were infected with KpU1 (UTI strain CG3 for survival experiment only) and KpL1 (liver abscess strain CG5) by tail-vein injection of 5 × 104 colony-forming units (CFU) bacterial suspension. The mice survival rates, cytokine level by enzyme-linked immunosorbent assay (ELISA), and bacterial presence in liver tissue by Giemsa stain were examined. The survival rates for the KpL1-infected animals were 28% and 0% in normal and diabetic groups, respectively, whereas, for the KpU1-infected mice, the rates were 100% and 75% during a 30-day observation. Nonsurviving KpL1-infected mice showed >105 bacteria/ml blood and the bacteria appeared in the liver sinus area and inside liver cells. The KpL1-infected mice showed a tendency to increase the blood interleukin 1β (IL-1β) level in both nondiabetic and diabetic groups, whereas the tumor necrosis factor-Α (TNF-Α) level was significantly decreased in the KpL1-infected diabetic mice (P = 0.002). In conclusion, the KP strain from liver abscess showed a greater virulence in mice than the KP from UTI and was more virulent in diabetic than in nondiabetic mice. The infection with KP from liver abscess significantly decreased the blood TNF-Α level in diabetes mellitus (DM) mice and the blood IL-1β level tended to increase in both infected nondiabetic and diabetic groups. High blood bacterial count and appearance of bacteria in liver sinus and cells usually contribute to death of the animals.

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Duan, Qiaoyan, Qi Wang, Shijun Sun, Qiaozhen Cui, Qi Ding, Ruobing Wang und Hui Wang. „ST11 Carbapenem-Resistant Klebsiella pneumoniae Clone Harboring blaNDM Replaced a blaKPC Clone in a Tertiary Hospital in China“. Antibiotics 11, Nr. 10 (07.10.2022): 1373. http://dx.doi.org/10.3390/antibiotics11101373.

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The nosocomial spread of carbapenem-resistant Enterobacterales (CRE) is extremely common, resulting in severe burdens on healthcare systems. In particular, the high-risk Klebsiella pneumoniae ST11 strain has a wide endemic area in China. The current study describes the results of continuous monitoring of CRE genotypes and phenotypes in a tertiary hospital in North China from 2012 to 2020. A total of 160 isolates were collected, including 109 Klebsiella. pneumoniae (68.13%), 29 Escherichia coli (26.60%), 12 Enterobacter cloacae (7.50%), and 10 other strains (6.25%). A total of 149 carbapenemase genes were detected, of which blaKPC-2 (51.0%) was the most common, followed by blaNDM-1 (22.82%), and blaNDM-5 (23.49%). Based on multi-locus sequence typing, the ST11 strain (66.1%) dominates K. pneumoniae, followed by ST15 (13.8%). Interestingly, the proportion of blaNDM (22.2%, 16/72) in ST11 K. pneumoniae was significantly increased in 2018–2019. Hence, whole-genome sequencing was performed on ST11 K. pneumoniae. Growth curves and in vitro competition experiments showed that K. pneumoniae carrying blaNDM exhibited a stronger growth rate (p < 0.001) and competition index (p < 0.001) than K. pneumoniae carrying blaKPC. Moreover, K. pneumoniae carrying blaNDM had a stronger biofilm-forming ability than K. pneumoniae carrying blaKPC (t = 6.578; p < 0.001). K. pneumoniae carrying blaKPC exhibited increased defense against bactericidal activity than K. pneumoniae carrying blaNDM. Thus, ST11 K. pneumoniae carrying blaNDM has strong adaptability and can locally replace K. pneumoniae carrying blaKPC to become an epidemic strain. Based on these findings, infection control and preventive measures should focus on the high-risk ST11-K. pneumoniae strain.

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Lai, Yi-Chyi, Shu-Li Yang, Hwei-Ling Peng und Hwan-You Chang. „Identification of Genes Present Specifically in a Virulent Strain of Klebsiella pneumoniae“. Infection and Immunity 68, Nr. 12 (01.12.2000): 7149–51. http://dx.doi.org/10.1128/iai.68.12.7149-7151.2000.

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ABSTRACT Klebsiella pneumoniae is a common cause of septicemia and urinary tract infections. The PCR-supported genomic subtractive hybridization was employed to identify genes specifically present in a virulent strain of K. pneumoniae. Analysis of 25 subtracted DNA clones has revealed 19 distinct nucleotide sequences. Two of the sequences were found to be the genes encoding the transposase of Tn3926 and a capsule polysaccharide exporting enzyme. Three sequences displayed moderate homology with bvgAS, which encodes a two-component signal transduction system in Bordetella pertussis. The rest of the sequences did not exhibit homology with any known genes. The distribution of these novel sequences varied greatly in K. pneumoniae clinical isolates, reflecting the heterogeneous nature of the K. pneumoniae population.

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Cheng, Yi-Hsiang, Tzu-Wen Huang, Chih-Han Juan, Sheng-Hua Chou, Yao-Yi Tseng, Ting-Wen Chen, Tsuey-Ching Yang und Yi-Tsung Lin. „Tigecycline-non-susceptible hypervirulent Klebsiella pneumoniae strains in Taiwan“. Journal of Antimicrobial Chemotherapy 75, Nr. 2 (08.11.2019): 309–17. http://dx.doi.org/10.1093/jac/dkz450.

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Abstract Objectives Emergent antimicrobial-resistant hypervirulent Klebsiella pneumoniae (hvKp) is an important public health issue. We aimed to investigate resistance mechanisms and hypervirulent traits among tigecycline-non-susceptible (TNS) K. pneumoniae clinical strains, focusing on one hvKp strain with in vivo evolution of tigecycline resistance. Methods TNS K. pneumoniae strains causing invasive diseases in a medical centre in Taiwan between July 2015 and April 2018 were collected. Resistance mechanisms were determined and hvKp strains were defined as rmpA/rmpA2-carrying strains. Isogenic strains with and without tigecycline resistance were subjected to WGS and in vivo virulence testing. Further, site-directed mutagenesis was used to confirm the resistance mechanism. Results In total, 31 TNS K. pneumoniae strains were isolated, including six hypervirulent strains. Tigecycline resistance mechanisms were mostly caused by overexpression of AcrAB and OqxAB together with up-regulation of RamA or RarA, respectively. One TNS hypervirulent strain (KP1692; MIC=6 mg/L) derived from its tigecycline-susceptible counterpart (KP1677; MIC=0.75 mg/L) showed acrAB overexpression. WGS revealed four genetic variations between KP1677 and KP1692. In addition, using site-directed mutagenesis, we confirmed that a 1 bp insertion in the ramA upstream region (RamR-binding site), leading to ramA and acrAB overexpression in KP1692, was responsible for tigecycline resistance. The in vivo virulence experiment showed that the TNS hvKp strain KP1692 still retained its high virulence compared with KP1677. Conclusions hvKp strains accounted for 19.4% among TNS strains. We identified alterations in the ramA upstream region as a mechanism of in vivo tigecycline resistance development in an hvKp strain.

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Cortés, Guadalupe, Dolores Álvarez, Carles Saus und Sebastián Albertí. „Role of Lung Epithelial Cells in Defense against Klebsiella pneumoniae Pneumonia“. Infection and Immunity 70, Nr. 3 (März 2002): 1075–80. http://dx.doi.org/10.1128/iai.70.3.1075-1080.2002.

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ABSTRACT The airway epithelium represents a primary site for the entry of pathogenic bacteria into the lungs. It has been suggested for many respiratory pathogens, including Klebsiella pneumoniae, that adhesion and invasion of the lung epithelial cells is an early stage of the pneumonia process. We observed that poorly encapsulated K. pneumoniae clinical isolates and an isogenic unencapsulated mutant invaded lung epithelial cells more efficiently than highly encapsulated strains independent of the K type. By contrast, the unencapsulated mutant was completely avirulent in a mouse model of pneumonia, unlike the wild-type strain, which produced pneumonia and systemic infection. Furthermore, the unencapsulated mutant bound more epithelially produced complement component C3 than the wild-type strain. Our results show that lung epithelial cells play a key role as a host defense mechanism against K. pneumoniae pneumonia, using two different strategies: (i) ingestion and control of the microorganisms and (ii) opsonization of the microorganisms. Capsular polysaccharide avoids both mechanisms and enhances the virulence of K. pneumoniae.

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Bator, Tomasz. „A New Laboratory Cultivation of Paramecium bursaria Using Non-Pathogenic Bacteria Strains“. Zeitschrift für Naturforschung C 65, Nr. 7-8 (01.08.2010): 479–82. http://dx.doi.org/10.1515/znc-2010-7-810.

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In most studies dealing with the laboratory cultivation of paramecia (Paramecium bursaria), Klebsiella pneumoniae bacteria are used to inoculate the medium. However, Klebsiella pneumoniae is a typical pathogen, and its use is always associated with a risk of infection. The aim of the present research was to examine non-pathogenic bacteria strains as components of the medium for Paramecium bursaria. The paramecia were incubated on lettuce infusions bacterized with different bacteria strains: Bacillus subtilis DSM 10, Bacillus megaterium DSM 32, Escherichia coli DSM 498, Micrococcus luteus DSM 348. A strain derived from the natural habitat of Paramecium bursaria was used as the control one. Experiments were conducted under constant light and in the dark. Paramecia cells were counted under a stereomicroscope on consecutive days of incubation. The obtained results show that the most intensive growth of Paramecium bursaria occurs in the presence of Escherichia coli DSM 498. The use of this strain as a component of the medium allows one to obtain a high number of ciliates regardless of the light conditions. It can be concluded that the Paramecium bursaria cultivation procedure can be modified by using the non-pathogenic bacteria strain Escherichia coli DSM 498 instead of Klebsiella pneumoniae.

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Lin, Yi-Tsung, Yi-Hsiang Cheng, Sheng-Hua Chou, Ying-Chi Huang und Liang Chen. „494. Fitness Cost of mcr-1-Mediated Colistin Resistance in Carbapenemase-Producing Klebsiella pneumoniae“. Open Forum Infectious Diseases 6, Supplement_2 (Oktober 2019): S241. http://dx.doi.org/10.1093/ofid/ofz360.563.

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Abstract Background The emergence of mobile colistin resistance gene mcr-1, a plasmid-borne polymyxin resistance mechanism, in carbapenem-resistant Klebsiella pneumoniae is an alarming concern. However, previous studies showed that the acquisition of mcr-1 was associated with a significant biological fitness cost in K. pneumoniae. We aimed to study the impact of mcr-1 on the biological fitness in clinical carbapenemase-producing K. pneumoniae strains. Methods Clinical carbapenemase-producing K. pneumoniae strains were collected consecutively at the Taipei Veterans General Hospital between November 2017 and December 2018. The strain positive for mcr-1 was subjected to whole-genome sequencing to delineate its genomic features. Escherichia coli J53 strain was used as the recipient strain in plasmid conjugation assay and the transconjugants were selected with sodium azide and colistin. Plasmid stability was tested by serial passaging in antibiotic-free LB broth for 28 days. The growth rate was compared between the parental mcr-1-bearing strain and the plasmid-cured strain. Results One ST11 strain isolated from a fatal case with bacteremia (KP2509) was found to harbor blaKPC-2, blaOXA-48, and mcr-1. This strain was resistant to colistin (MIC=8 mg/L) and imipenem (MIC≥16 mg/L). Whole-genome sequencing of KP2509 showed that mcr-1, blaKPC-2 and blaOXA-48 were located on an IncHI-FIB type plasmid of 319 Kb, an IncFII type plasmid of 96 Kb, and an IncL type plasmid of 64 Kb, respectively. Conjugation efficiency of mcr-1-bearing plasmid was 2.24 × 10–4, and the colistin MIC of E. coli J53 transconjugant increased from 0.5 to 8 mg/L. The mcr-1-bearing plasmid in KP2509 showed high plasmid stability, and only ~1% were lost after 27-day passages. The resulting plasmid-cured strain (PC-KP2509) was susceptible to colistin (MIC=0.5 mg/L) and had a similar growth rate to that of parental mcr-1-bearing strain KP2509. Conclusion We identified an ST11 K. pneumoniae strain carrying blaKPC-2, blaOXA-48, and mcr-1 genes causing a fatal bacteremia. The large mcr-1-bearing plasmid confers a moderate level of colistin resistance but without significant biological fitness cost in carbapenemase-producing K. pneumoniae, which could result in a serious threat clinically. Disclosures All authors: No reported disclosures.

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Wei, Dong, Yuki Yuminaga, Jiping Shi und Jian Hao. „Non-capsulated mutants of a chemical-producing Klebsiella pneumoniae strain“. Biotechnology Letters 40, Nr. 4 (10.02.2018): 679–87. http://dx.doi.org/10.1007/s10529-018-2524-5.

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Szabo, Orsolya, Bela Kocsis, Nikolett Szabo, Katalin Kristof und Dora Szabo. „Contribution of OqxAB Efflux Pump in Selection of Fluoroquinolone-Resistant Klebsiella pneumoniae“. Canadian Journal of Infectious Diseases and Medical Microbiology 2018 (23.09.2018): 1–5. http://dx.doi.org/10.1155/2018/4271638.

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The role of OqxAB efflux pump in Klebsiella pneumoniae was investigated in correlation with ciprofloxacin exposure. K. pneumoniae SE23 and K. pneumoniae SE191 were isolated from urinary tract infections and were analyzed in this study. Each carried oqxAB resistance determinant and exhibited ciprofloxacin MIC of 0.06 and 0.5 mg/L, respectively. Tested strains were initially exposed to their ciprofloxacin MIC values for 24 hours. Later on, the ciprofloxacin exposition has been increased to a daily 1, 2, 4, and to a final 8 mg/L. Total cellular RNA was extracted at 30, 60, 90, and 120 minutes of initial exposure and after every 24 hours. Quantitative reverse-transcriptase PCR was performed from each RNA sample. Mutation in gyrA and parC genes was analyzed in each strain and multilocus sequence typing (MLST) was performed. Ciprofloxacin exposure selected resistant strain from K. pneumoniae SE191; by contrast, K. pneumoniae SE23 was not adjustable to the increasing ciprofloxacin concentrations. During initial exposure, both oqxA and oqxB expression remained low (2−ΔCt = 1-2.03). However, increasing ciprofloxacin promoted oqxB expression as it reached fold increase of 15.8–22.8, while oqxA expression was maintained (2−ΔCt = 2-2.15). An amino acid substitution Ser83Tyr in gyrA was detected in K. pneumoniae SE191, but no additional mutations occurred as consequence to ciprofloxacin exposure. MLST identified K. pneumoniae SE191 as ST274, while K. pneumoniae SE23 belonged to the novel ST2567. Ciprofloxacin concentration-dependent upregulation of oqxAB efflux pump in K. pneumoniae is clonally related and contributes to selection for higher level of fluoroquinolone resistance.

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Bidet, Philippe, Béatrice Burghoffer, Valérie Gautier, Naïma Brahimi, Patricia Mariani-Kurkdjian, Alaa El-Ghoneimi, Edouard Bingen und Guillaume Arlet. „In Vivo Transfer of Plasmid-Encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an Infant and Selection of Impermeability to Imipenem in K. pneumoniae“. Antimicrobial Agents and Chemotherapy 49, Nr. 8 (August 2005): 3562–65. http://dx.doi.org/10.1128/aac.49.8.3562-3565.2005.

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ABSTRACT We describe in vivo selection of a Klebsiella pneumoniae strain with diminished imipenem susceptibility attributable to plasmid-encoded ACC-1 β-lactamase production and loss of a 36-kDa major outer membrane protein, together with transfer of this plasmid from K. pneumoniae to Escherichia coli in a Tunisian infant.

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Zhang, Cheng, Qingkai Hao, Zhengyi Zhang, Xianghui Zhang, Hongyu Pan, Jiahuan Zhang, Hao Zhang und Fengjie Sun. „Whole Genome Sequencing and Analysis of Chlorimuron-Ethyl Degrading Bacteria Klebsiella pneumoniae 2N3“. International Journal of Molecular Sciences 20, Nr. 12 (22.06.2019): 3053. http://dx.doi.org/10.3390/ijms20123053.

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Klebsiella pneumoniae 2N3 is a strain of gram-negative bacteria that can degrade chlorimuron-ethyl and grow with chlorimuron-ethyl as the sole nitrogen source. The complete genome of Klebsiella pneumoniae 2N3 was sequenced using third generation high-throughput DNA sequencing technology. The genomic size of strain 2N3 was 5.32 Mb with a GC content of 57.33% and a total of 5156 coding genes and 112 non-coding RNAs predicted. Two hydrolases expressed by open reading frames (ORFs) 0934 and 0492 were predicted and experimentally confirmed by gene knockout to be involved in the degradation of chlorimuron-ethyl. Strains of ΔORF 0934, ΔORF 0492, and wild type (WT) reached their highest growth rates after 8–10 hours in incubation. The degradation rates of chlorimuron-ethyl by both ΔORF 0934 and ΔORF 0492 decreased in comparison to the WT during the first 8 hours in culture by 25.60% and 24.74%, respectively, while strains ΔORF 0934, ΔORF 0492, and the WT reached the highest degradation rates of chlorimuron-ethyl in 36 hours of 74.56%, 90.53%, and 95.06%, respectively. This study provides scientific evidence to support the application of Klebsiella pneumoniae 2N3 in bioremediation to control environmental pollution.

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Abdelwahab, Radwa, Munirah M. Alhammadi, Ehsan A. Hassan, Entsar H. Ahmed, Nagla H. Abu-Faddan, Enas A. Daef, Stephen J. W. Busby und Douglas F. Browning. „Antimicrobial Resistance and Comparative Genome Analysis of Klebsiella pneumoniae Strains Isolated in Egypt“. Microorganisms 9, Nr. 9 (05.09.2021): 1880. http://dx.doi.org/10.3390/microorganisms9091880.

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Klebsiella pneumoniae is an important human pathogen in both developing and industrialised countries that can causes a variety of human infections, such as pneumonia, urinary tract infections and bacteremia. Like many Gram-negative bacteria, it is becoming resistant to many frontline antibiotics, such as carbapenem and cephalosporin antibiotics. In Egypt, K. pneumoniae is increasingly recognised as an emerging pathogen, with high levels of antibiotic resistance. However, few Egyptian K. pneumoniae strains have been sequenced and characterised. Hence, here, we present the genome sequence of a multidrug resistant K. pneumoniae strain, KPE16, which was isolated from a child in Assiut, Egypt. We report that it carries multiple antimicrobial resistance genes, including a blaNDM-1 carbapenemase and extended spectrum β-lactamase genes (i.e., blaSHV-40, blaTEM-1B, blaOXA-9 and blaCTX-M-15). By comparing this strain with other Egyptian isolates, we identified common plasmids, resistance genes and virulence determinants. Our analysis suggests that some of the resistance plasmids that we have identified are circulating in K. pneumoniae strains in Egypt, and are likely a source of antibiotic resistance throughout the world.

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Ilyashenko, A. N. „The effect of Bacillus in the composition of the probiotic Natupro® against bacterial pathogenic strains in vitro“. Agrarian science, Nr. 3 (25.03.2024): 80–84. http://dx.doi.org/10.32634/0869-8155-2024-380-3-80-84.

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Relevance. The use of probiotics for health benefits is becoming popular because of the quest for safer products with protective and therapeutic effects against diseases and infectious agents. The emergence and spread of antimicrobial resistance among pathogens had prompted restrictions over the nontherapeutic use of antibiotics for prophylaxis and growth promotion, especially in animal husbandry. While single-strain probiotics are beneficial to health, multi-strain probiotics might be more helpful because of synergy and additive effects among the individual isolates.Methods. In this study, the effectiveness of the multi-strain probiotic Natupro on pathogenic bacterial strains (Escherichia coli 320, Salmonella typhimurium 415, Staphylococcus aureus 12600, Streptococcus uberis 700407, Klebsiella pneumoniae 13883, Listeria monocitogenes 766/20) in vitro was studied.Results. According to the results of the work carried out, it was found that the bacterial strains in Natupro® exhibit lytic activity against pathogenic strains of Salmonella typhimurium 415 and Klebsiella pneumoniae 13883 and have a bacteriostatic effect on Escherichia coli strain 320 and Salmonella typhimurium 415.

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Hussain, Rahma Ali Alhadi, Ashwak Jasim Kzar und Alaa Salim Hamza. „Detection Sequencing NDM and blaOXA Genes in Metallo-$\beta$-Lactamase Producing Klebsiella Pneumoniae“. Journal of Pioneering Medical Science 12, Nr. 3 (31.12.2023): 36–40. http://dx.doi.org/10.61091/jpms20231238.

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The prevalence of Carbapenem-resistance in Enterobacteriaceae is rising worldwide and poses a treatment challenge for healthcare facilities. This study aims to detect a sequence of New Delhi metallo- -lactamase (NDM) and bla OXA-48 gene in metalo-beta-lactamase-producing Klebsiella pneumoniae isolated from a few hospitals in the city of Baghdad. 74 Klebsiella pneumoniae were isolated from 320 clinical samples that were routinely submitted to the diagnostic laboratory of the chosen hospital. These samples included sputum, tissue swabs, folly tips, urine, ear swabs, bloodstream, pus, wound swabs, endotracheal tubes, and body fluids. In this study, 74 isolates were examined for expression of carbapenemase by phenotyping method. The modified Hodge Test (MHT) is utilized for phenotypic detection. MHT was positive for 24 carbapenem-resistant Klebsiella pneumoniae. Genotyping was accomplished by using polymerase chain reaction (PCR) to amplify target genes by employing particular primers. The NDM gene was detected in 19/24 isolates, and the blaOXA-48 gene was detected in 5/24 isolates. The presence of bla NDM and the dangerously high frequency of MBL in the Klebsiella pneumoniae strain are both concerning, and it has been demonstrated to be challenging to treat and inhibit infection.

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Lin, Di, Jian Chen, Yan Yang, Jun Cheng und Changgui Sun. „Epidemiological study of carbapenem-resistant Klebsiella pneumoniae“. Open Medicine 13, Nr. 1 (16.10.2018): 460–66. http://dx.doi.org/10.1515/med-2018-0070.

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AbstractBackgroundThis research is aimed to study the resistance and molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP).Methodology38 isolated CRKP strains were collected from clinical specimens.ResultsThe resistance rates were more than 70.0%. Ampicillin had the highest rates among them (100.0%). 34 strains (89.5%) among the 38 CRKP strains carried blaKPC-2 gene, and 3 strains (7.9%) carried blaIMP-4 gene. 36 strains (94.7%) among the 38 CRKP strains carried blaSHV gene, 29 strains (76.3%) carried blaTEM gene, and 26 strains (68.4%) carried blaCT-M gene. 7 strains (18.4%) among the 38 CRKP strains carried blaDHA-1 gene. 15 strains (39.5%) in 38 CRKP strains lost two fenestra proteins, ompK35 and ompK36, and the rest 23 strains carried ompK36 genes. 38 CRKP strains were divided into five kinds of ST types, with ST11 type as the most (86.8%, 33/38). The rest of the ST types included 2 strains of ST23 (5.3%, 2/38), one strain of ST15, ST1373 and ST1415 (2.6%, 1/38).ConclusionsCRKP resistance is severe, and the mechanism of drug resistance has become increasingly complex. Various ST types and resistance genes are related to CRKP. The clinical prevention and control work is imminent.

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Padilla, Emma, Enrique Llobet, Antonio Doménech-Sánchez, Luis Martínez-Martínez, José Antonio Bengoechea und Sebastián Albertí. „Klebsiella pneumoniae AcrAB Efflux Pump Contributes to Antimicrobial Resistance and Virulence“. Antimicrobial Agents and Chemotherapy 54, Nr. 1 (26.10.2009): 177–83. http://dx.doi.org/10.1128/aac.00715-09.

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ABSTRACT Respiratory infections caused by Klebsiella pneumoniae are characterized by high rates of mortality and morbidity. Management of these infections is often difficult, due to the high frequency of strains that are resistant to multiple antimicrobial agents. Multidrug efflux pumps play a major role as a mechanism of antimicrobial resistance in Gram-negative pathogens. In the present study, we investigated the role of the K. pneumoniae AcrRAB operon in antimicrobial resistance and virulence by using isogenic knockouts deficient in the AcrB component and the AcrR repressor, both derived from the virulent strain 52145R. We demonstrated that the AcrB knockout was more susceptible, not only to quinolones, but also to other antimicrobial agents, including β-lactams, than the wild-type strain and the AcrR knockout. We further showed that the AcrB knockout was more susceptible to antimicrobial agents present in human bronchoalveolar lavage fluid and to human antimicrobial peptides than the wild-type strain and the AcrR knockout. Finally, the AcrB knockout exhibited a reduced capacity to cause pneumonia in a murine model, in contrast to the wild-type strain. The results of this study suggest that, in addition to contributing to the multidrug resistance phenotype, the AcrAB efflux pump may represent a novel virulence factor required for K. pneumoniae to resist innate immune defense mechanisms of the lung, thus facilitating the onset of pneumonia.

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Doménech-Sánchez, Antonio, Luis Martínez-Martínez, Santiago Hernández-Allés, María del Carmen Conejo, Álvaro Pascual, Juan M. Tomás, Sebastián Albertí und Vicente Javier Benedí. „Role of Klebsiella pneumoniae OmpK35 Porin in Antimicrobial Resistance“. Antimicrobial Agents and Chemotherapy 47, Nr. 10 (Oktober 2003): 3332–35. http://dx.doi.org/10.1128/aac.47.10.3332-3335.2003.

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ABSTRACT OmpK35 from Klebsiella pneumoniae is the homologue of Escherichia coli OmpF porin. Expression of OmpK35 in K. pneumoniae strain CSUB10R (lacking both OmpK35 and OmpK36) decreased the MICs of cephalosporins and meropenem ≥128-fold and decreased the MICs of imipenem, ciprofloxacin, and chloramphenicol ≥8-fold. MIC reductions by OmpK35 were 4 times (cefepime), 8 times (cefotetan, cefotaxime, and cefpirome), or 128 times (ceftazidime) higher than those caused by OmpK36, but the MICs were similar or 1 dilution lower for other evaluated agents.

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Yang, Chen, Miaomiao Yang, Wanhua Zhao, Yue Ding, Yu Wang und Jian Li. „Establishing a Klebsiella pneumoniae-Based Cell-Free Protein Synthesis System“. Molecules 27, Nr. 15 (22.07.2022): 4684. http://dx.doi.org/10.3390/molecules27154684.

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Cell-free protein synthesis (CFPS) systems are emerging as powerful platforms for in vitro protein production, which leads to the development of new CFPS systems for different applications. To expand the current CFPS toolkit, here we develop a novel CFPS system derived from a chassis microorganism Klebsiella pneumoniae, an important industrial host for heterologous protein expression and the production of many useful chemicals. First, we engineered the K. pneumoniae strain by deleting a capsule formation-associated wzy gene. This capsule-deficient strain enabled easy collection of the cell biomass for preparing cell extracts. Then, we optimized the procedure of cell extract preparation and the reaction conditions for CFPS. Finally, the optimized CFPS system was able to synthesize a reporter protein (superfolder green fluorescent protein, sfGFP) with a maximum yield of 253 ± 15.79 μg/mL. Looking forward, our K. pneumoniae-based CFPS system will not only expand the toolkit for protein synthesis, but also provide a new platform for constructing in vitro metabolic pathways for the synthesis of high-value chemicals.

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Ali, Fawad, Sajid Hussain, Farmanullah Khan, Muhamad Faheem, Neelum Gul Qazi, Hamza Hussain Bangash und Zahra Batool Manzoor. „Horizontal Transfer of Plasmid Mediated Carbapenem Resistant Genes from Klebsiella pneumoniae to Escherichia coli“. Molecular Medicine Communications 2, Nr. 02 (31.12.2022): 135–47. http://dx.doi.org/10.55627/mmc.002.002.0145.

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Carbapenem resistance among Enterobacteriaceae is an emerging problem worldwide. Klebsiella pneumonia carbapenemase (blaKPC) enzymes are amongst the most common beta-lactamases that have been studied. This broad group of organisms with various resistance mechanisms is associated with increased lengths of hospitalization, costs of medical care, morbidity, and mortality. The global spread of Carbapenem Resistance Enterobacteriaceae (CRE) has largely been attributed to disseminating a dominant strain of Klebsiella pneumoniae producing a serine beta-lactamase, termed as Klebsiella pneumonia carbapenemase (KPC). This study was designed to determine in vitro efficacy of horizontal transfer of plasmid-mediated carbapenem-resistant genes from Klebsiella pneumoniae to Escherichia coli. A total of 30 gram-negative rods from different clinical samples were taken. After preliminary antimicrobial susceptibility screening, isolates that showed a resistance zone were included in the study. These isolates were then subjected to the Modified Hodge test for confirmation of carbapenemase production. Out of 10 isolates, four were positive for carbapenemase production. The plasmid from these resistant strains was transformed into DH5α. RFLP analysis was carried to evaluate these transgenic cells resulted in horizontal gene transfer of resistant plasmid DNA from Klebsiella pneumoniae to Escherichia coli.

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Nang, Sue C., Faye C. Morris, Michael J. McDonald, Mei-Ling Han, Jiping Wang, Richard A. Strugnell, Tony Velkov und Jian Li. „Fitness cost of mcr-1-mediated polymyxin resistance in Klebsiella pneumoniae“. Journal of Antimicrobial Chemotherapy 73, Nr. 6 (05.03.2018): 1604–10. http://dx.doi.org/10.1093/jac/dky061.

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Abstract Objectives The discovery of mobile colistin resistance mcr-1, a plasmid-borne polymyxin resistance gene, highlights the potential for widespread resistance to the last-line polymyxins. In the present study, we investigated the impact of mcr-1 acquisition on polymyxin resistance and biological fitness in Klebsiella pneumoniae. Methods K. pneumoniae B5055 was used as the parental strain for the construction of strains carrying vector only (pBBR1MCS-5) and mcr-1 recombinant plasmids (pmcr-1). Plasmid stability was determined by serial passaging for 10 consecutive days in antibiotic-free LB broth, followed by patching on gentamicin-containing and antibiotic-free LB agar plates. Lipid A was analysed using LC–MS. The biological fitness was examined using an in vitro competition assay analysed with flow cytometry. The in vivo fitness cost of mcr-1 was evaluated in a neutropenic mouse thigh infection model. Results Increased polymyxin resistance was observed following acquisition of mcr-1 in K. pneumoniae B5055. The modification of lipid A with phosphoethanolamine following mcr-1 addition was demonstrated by lipid A profiling. The plasmid stability assay revealed the instability of the plasmid after acquiring mcr-1. Reduced in vitro biological fitness and in vivo growth were observed with the mcr-1-carrying K. pneumoniae strain. Conclusions Although mcr-1 confers a moderate level of polymyxin resistance, it is associated with a significant biological fitness cost in K. pneumoniae. This indicates that mcr-1-mediated resistance in K. pneumoniae could be attenuated by limiting the usage of polymyxins.

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Weng, Xingbei, Qiucheng Shi, Sheng Wang, Yubo Shi, Dinghe Sun und Yunsong Yu. „The Characterization of OXA-232 Carbapenemase-Producing ST437 Klebsiella pneumoniae in China“. Canadian Journal of Infectious Diseases and Medical Microbiology 2020 (08.07.2020): 1–5. http://dx.doi.org/10.1155/2020/5626503.

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Carbapenem-resistant Klebsiella pneumoniae (CRKP) was epidemic around the world and become a global threat to public health. The most important carbapenem-resistant mechanism is producing carbapenemases, especially Klebsiella pneumoniae carbapenemase (KPC), which is prevalent in the international clonal complex CC11. The high-risk multidrug-resistant CC11 is widespread worldwide, and KPC-producing and (New Delhi metallo) NDM-producing strains had been reported in this clonal complex before; moreover, cases with the CC11 strain faced more severe forms of drug resistance and treatment challenges than other clonal complexes. In this study, we identified an OXA-232-producing ST437 Klebsiella pneumoniae isolate in China, which belonged to CC11. The isolate was resistant to β-lactams, aminoglycosides, and fluoroquinolones but susceptible to fosfomycin, tigecycline, and colistin. The blaOXA-232 gene was located on a 6141 bp ColKP3-type nonconjugative plasmid, and the plasmid was transformed by chemical transformation successfully. This is the first report of OXA-232-producing ST437 K. pneumoniae in China, a new clone of high-risk multidrug-resistant CC11.

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Ventura, Anna, Elena Addis, Anna Bertoncelli und Annarita Mazzariol. „Multiple detection of hypermucoviscous and hypervirulent strains of Klebsiella pneumoniae: An emergent health care threat“. Acta Microbiologica et Immunologica Hungarica 69, Nr. 4 (06.12.2022): 297–302. http://dx.doi.org/10.1556/030.2022.01908.

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AbstractThis study focused on the characterization of 19 hypermucoviscous Klebsiella pneumoniae strains, that were identified from 26 hypermucosal strains. In order to identify hypermucoviscous strains of K. pneumoniae, the string test was applied. This phenotype is known in the literature as one of the virulence factors of this species together with the production of biofilm and other hypervirulence factor genes such as: rmpA, rmpA2, iucA, iroB, peg-344. We also investigated presence of magA gene that correlates with the hyper-production of capsule of K1 serotype. Of the strains under study, 13 out of 19 harboured at least one virulence factor.Sequence type (ST) was determined in order to identify known high-risk clones or new emerging high-risk clones and their variability in a single clinical setting. Important STs found among these strains were ST65 and ST29. Carbapenem resistance was also investigated and 4 out of 19 strains harboured at least a carbapenemase: one strain harboured a KPC enzyme alone, one strain carried a KPC and an OXA-48 like, one strain produced OXA-48-like alone, and the last strain harboured two metallo-β-lactamases (VIM-1 and NDM-5) plus OXA-48-like. In particular, this latter strain belongs to ST383, which was recently reported in Northern Italy as a hypervirulent and XDR strain.The global spread of hypervirulent K. pneumoniae is an important epidemiological issue that should be considered in diagnostic and therapeutic managements of patients with K. pneumoniae infections.

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Borkar, S. G., und T. S. Ajayasree. „Reaction of Indian Chicks to Klebsiella Pneumoniae Strain Borkar, a Plant Pathogen Causing Pneumonia in Plants“. European Journal of Environment and Earth Sciences 1, Nr. 4 (24.07.2020). http://dx.doi.org/10.24018/ejgeo.2020.1.4.26.

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Klebsiella pneumoniae is emerging as an important bacterial plant pathogen in Asia region particularly in China and India. The Chinese strain of Klebsiella pneumoniae causing top rot of maize is reported to be pathogenic on mice also. The Indian strain of Klebsiella pneumoniae causing root bark necrosis and wilt in pomegranate is found non- pathogenic on Indian bird chicken, thereby indicating it to be plant host specific.

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Pei, Na, Zijuan Jian, Yirui Liu, Tianzhu Liang, Wenen Liu und Junhua Li. „Draft Genome Sequence of a Polymyxin-Resistant Klebsiella pneumoniae Clinical Strain Carrying mcr-8.1 and bla NDM-5“. Microbiology Resource Announcements 10, Nr. 12 (25.03.2021). http://dx.doi.org/10.1128/mra.01224-20.

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Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major threat to global health. Here, we report the draft genome sequence of a Klebsiella pneumoniae clinical strain carrying mcr-8.1 and bla NDM-5 .

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Budnick, James A., X. Renee Bina und James E. Bina. „Complete Genome Sequence of Klebsiella pneumoniae Strain ATCC 43816“. Microbiology Resource Announcements 10, Nr. 5 (04.02.2021). http://dx.doi.org/10.1128/mra.01441-20.

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Klebsiella pneumoniae is a member of Enterobacteriaceae that causes a multitude of infections in compromised and healthy individuals. The rise of hypervirulent and multiple-drug-resistant K. pneumoniae strains has made this organism a global health threat. Here, we report the complete genome sequence of K. pneumoniae strain ATCC 43816.

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Chen, Liang, Barun Mathema, Johann D. D. Pitout, Frank R. DeLeo und Barry N. Kreiswirth. „Epidemic Klebsiella pneumoniae ST258 Is a Hybrid Strain“. mBio 5, Nr. 3 (24.06.2014). http://dx.doi.org/10.1128/mbio.01355-14.

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ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE), especially Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae, pose an urgent threat in health facilities in the United States and worldwide. K. pneumoniae isolates classified as sequence type 258 (ST258) by multilocus sequence typing are largely responsible for the global spread of KPC. A recent comparative genome study revealed that ST258 K. pneumoniae strains are two distinct genetic clades; however, the molecular origin of ST258 largely remains unknown, and our understanding of the evolution of the two genetic clades is incomplete. Here we compared the genetic structures and single-nucleotide polymorphism (SNP) distributions in the core genomes of strains from two ST258 clades and other STs (ST11, ST442, and ST42). We identified an ~1.1-Mbp region on ST258 genomes that is homogeneous to that of ST442, while the rest of the ST258 genome resembles that of ST11. Our results suggest ST258 is a hybrid clone—80% of the genome originated from ST11-like strains and 20% from ST442-like strains. Meanwhile, we sequenced an ST42 strain that carries the same K-antigen-encoding capsule polysaccharide biosynthesis gene (cps) region as ST258 clade I strains. Comparison of the cps-harboring regions between the ST42 and ST258 strains (clades I and II) suggests the ST258 clade I strains evolved from a clade II strain as a result of cps region replacement. Our findings unravel the molecular evolution history of ST258 strains, an important first step toward the development of diagnostic, therapeutic, and vaccine strategies to combat infections caused by multidrug-resistant K. pneumoniae. IMPORTANCE Recombination events and replacement of chromosomal regions have been documented in various bacteria, and these events have given rise to successful pathogenic clones. Here we used comparative genomic analyses to discover that the ST258 K. pneumoniae genome is a hybrid—80% of the chromosome is homologous to ST11 strains, while the remaining 20% is homologous to that of ST442. Meanwhile, a recent study indicated that ST258 strains can be segregated into two ST258 clades, with distinct capsule polysaccharide gene (cps) regions. Our analysis suggests ST258 clade I strains evolved from clade II through homologous recombination of cps region. Horizontal transfer of the cps region appears to be a key element driving the molecular diversification in K. pneumoniae strains. These findings not only extend our understanding of the molecular evolution of ST258 but are an important step toward the development of effective control and treatment strategies for multidrug-resistant K. pneumoniae.

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Granata, Guido, und Nicola Petrosillo. „Resistance to colistin in Klebsiella pneumoniae: a 4.0 strain?“ Infectious Disease Reports 9, Nr. 2 (31.05.2017). http://dx.doi.org/10.4081/idr.2017.7104.

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The global rise of multidrug-resistant gram-negative bacteria represents an increasing threat to patient safety. From the first observation of a carbapenem-resistant gramnegative bacteria a global spread of extendedspectrum beta-lactamases and carbapenemases producing Klebsiella pneumoniae has been observed. Treatment options for multidrug-resistant K. pneumoniae are actually limited to combination therapy with some aminoglycosides, tigecycline and to older antimicrobial agents. Unfortunately, the prevalence of colistin-resistant and tigecycline- resistant K. pneumoniae is increasing globally. Infection due to colistin-resistant K. pneumoniae represents an independent risk factor for mortality. Resistance to colistin in K. pneumoniae may be multifactorial, as it is mediated by chromosomal genes or plasmids. The emergence of transmissible, plasmidmediated colistin resistance is an alarming finding. The absence of new agents effective against resistant Gram-negative pathogens means that enhanced surveillance, compliance with infection prevention procedures, and antimicrobial stewardship programs will be required to limit the spread of colistinresistant K. pneumoniae.

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Yang, Xue-Jing, Sai Wang, Jun-Min Cao und Jia-Hui Hou. „Draft Genome Sequence of Klebsiella pneumoniae Strain AS Isolated from Zhejiang Provincial Hospital of TCM, China“. Genome Announcements 4, Nr. 5 (22.09.2016). http://dx.doi.org/10.1128/genomea.00930-16.

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Klebsiella pneumoniae is a Gram-negative, nonmotile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium. Here we present draft genome assemblies of Klebsiella pneumoniae AS, which was isolated in China. The genomic information will provide a better understanding of the physiology, adaptation, and evolution of K. pneumoniae.

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„Klebsiella Distinctive Syndrome Presenting with Muscular Abscess and Osteomyelitis: Case Report“. Journal of the Medical Association of Thailand 103, Nr. 11 (15.11.2020): 1236–39. http://dx.doi.org/10.35755/jmedassocthai.2020.11.11111.

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The present article describes a case of 46-year-old man who presented with progressive pain and swelling at the right lower back and buttock for three weeks. The investigation showed an abscess at the right lower back and buttock, osteomyelitis of the iliac bone, and a hepatic abscess. The causative pathogens were confirmed to be Klebsiella pneumoniae, both K1 and K2 serotype. This patient was treated with surgical management, percutaneous drainage, intravenous antibiotic, and control of his underlying disease risk factor, which was diabetic mellitus. Keywords: Klebsiella distinctive syndrome, Klebsiella liver abscess syndrome, Hypervirulent strain of Klebsiella pneumoniae, Klebsiella pneumoniae, Liver abscess

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Carl, Gaby, Claudia Jäckel, Josephine Grützke, Stefan Hertwig, Mirjam Grobbel, Burkhard Malorny, Jörg Rau, Annemarie Käsbohrer und Jens A. Hammerl. „Complete Genome Sequence of the Temperate Klebsiella pneumoniae Phage KPP5665-2“. Genome Announcements 5, Nr. 43 (26.10.2017). http://dx.doi.org/10.1128/genomea.01118-17.

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ABSTRACT We describe here the genome sequence of the novel temperate Klebsiella pneumoniae phage KPP5665-2 isolated from a Klebsiella pneumoniae strain recovered from milk in Germany in 2016. The phage exhibited a narrow host range and a siphoviridal morphology. KPP5665-2-related prophage sequences were detected in whole-genome sequencing (WGS) data of various Klebsiella species isolates.

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Jousset, Agnès B., Saoussen Oueslati, Cécile Emeraud, Rémy A. Bonnin, Laurent Dortet, Bogdan I. Iorga und Thierry Naas. „KPC-39-Mediated Resistance to Ceftazidime-Avibactam in a Klebsiella pneumoniae ST307 Clinical Isolate“. Antimicrobial Agents and Chemotherapy 65, Nr. 12 (17.11.2021). http://dx.doi.org/10.1128/aac.01160-21.

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Resistance to the ceftazidime (CAZ)-avibactam (AVI) combination is increasingly being reported. Here, we report a CAZ-AVI-resistant Klebsiella pneumoniae strain belonging to the high-risk sequence type 307 (ST307) clone and producing Klebsiella pneumoniae carbapenemase 39 (KPC-39), a single-amino-acid variant of KPC-3 (A172T).

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Tambassi, Martina, Elena Passarini, Ilaria Menozzi, Melissa Berni, Chiara Bracchi, Alessandra Dodi, Luca Bolzoni et al. „Klebsiella pneumoniae carrying multiple alleles of antigen 43-encoding gene of Escherichia coli associated with biofilm formation“. European Journal of Clinical Microbiology & Infectious Diseases, 25.01.2023. http://dx.doi.org/10.1007/s10096-023-04552-6.

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AbstractA clinical strain of Klebsiella pneumoniae typed as sequence type 307 carrying three different alleles of the flu gene encoding the Escherichia coli virulence factor antigen 43 associated with biofilm formation was detected and characterized. The flu alleles are located in the chromosome inside putative integrative conjugative elements. The strain displays the phenotypes associated with Ag43, i.e. bi-phasic colony morphology and enhanced biofilm production. Furthermore, the strain produces low amount of capsule known to affect Ag43 function. Analysis of 1431 worldwide deposited genomes revealed that 3.7% Klebsiella pneumoniae carry one or two flu alleles.

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Fang, Jianhua, Guoyu Wang, Xiuhua Kang, Zhenhui Pan, Yanfang Mei, Huade Chen, Yang Liu und Tianxin Xiang. „Analysis of the hypovirulent Klebsiella pneumoniae with the NDM-5 gene on IncN plasmids“. Microbiology Spectrum, 29.11.2023. http://dx.doi.org/10.1128/spectrum.03443-23.

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ABSTRACT Our study aims to analyze the hypovirulent Klebsiella pneumoniae with the NDM-5 gene on IncN plasmids. This strain was isolated from a patient with a liver abscess, and the ST type was ST147. The characterization, antimicrobial drug resistance, and virulence of the strain with the NDM-5 gene on IncN plasmids were analyzed by whole-genome sequencing. Virulence testing was conducted using the Galleria mellonella infection model, serum killing, and biofilm formation assay. Growth measurement analysis, an in vitro competition experiment, and a plasmid stability assay were calculated using fitness costs. A conjugation assay was used to test NDM-5 transfer between bacterial strains. For statistical analysis and image visualization, GraphPad Prism 8.0 was employed. The result revealed that the hypovirulent strain with the NDM-5 gene was located on the IncN plasmid (92.284 kb), which is rare in Klebsiella pneumoniae . Whole-genome sequencing revealed the presence of 25 and 22 resistance and virulence genes, respectively. The strain KP4089 was resistant to carbapenems, β-lactam, ceftazidime-avibactam, and macrolides but remained susceptible to aminoglycosides, mucilage, and tigecycline in antimicrobial susceptibility tests. Furthermore, the strain KP4089 exhibited significantly lower serum killing and biofilm formation rates, demonstrating that the strain with the NDM-5 gene had low virulence. The strain with the NDM-5 gene on the IncN plasmid was genetically stable and had no fitness cost associated with identifying IncN plasmids. Future research should focus on clinical surveillance of hypovirulent multidrug-resistant Klebsiella pneumoniae to identify the potential strain and mechanistic studies to prevent its spread. IMPORTANCE It is crucial to strengthen the ongoing clinical surveillance of non-highly virulent, multi-resistant Klebsiella pneumoniae .

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Gilcrease, Eddie B., Sherwood R. Casjens, Ananda Bhattacharjee und Ramesh Goel. „A Klebsiella pneumoniae NDM-1+ bacteriophage: Adaptive polyvalence and disruption of heterogenous biofilms“. Frontiers in Microbiology 14 (17.02.2023). http://dx.doi.org/10.3389/fmicb.2023.1100607.

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Bacteriophage KL-2146 is a lytic virus isolated to infect Klebsiella pneumoniae BAA2146, a pathogen carrying the broad range antibiotic resistance gene New Delhi metallo-betalactamase-1 (NDM-1). Upon complete characterization, the virus is shown to belong to the Drexlerviridae family and is a member of the Webervirus genus located within the (formerly) T1-like cluster of phages. Its double-stranded (dsDNA) genome is 47,844 bp long and is predicted to have 74 protein-coding sequences (CDS). After challenging a variety of K. pneumoniae strains with phage KL-2146, grown on the NDM-1 positive strain BAA-2146, polyvalence was shown for a single antibiotic-sensitive strain, K. pneumoniae 13,883, with a very low initial infection efficiency in liquid culture. However, after one or more cycles of infection in K. pneumoniae 13,883, nearly 100% infection efficiency was achieved, while infection efficiency toward its original host, K. pneumoniae BAA-2146, was decreased. This change in host specificity is reversible upon re-infection of the NDM-1 positive strain (BAA-2146) using phages grown on the NDM-1 negative strain (13883). In biofilm infectivity experiments, the polyvalent nature of KL-2146 was demonstrated with the killing of both the multidrug-resistant K. pneumoniae BAA-2146 and drug-sensitive 13,883 in a multi-strain biofilm. The ability to infect an alternate, antibiotic-sensitive strain makes KL-2146 a useful model for studying phages infecting the NDM-1+ strain, K. pneumoniae BAA-2146.

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Lin, Wei-Hung, Po-Xing Zheng, Tsunglin Liu, Chin-Chung Tseng, Wei-Chu Chen, Ming-Cheng Wang und Jiunn-Jong Wu. „Complete Genome Sequence of Community-Acquired Klebsiella pneumoniae KP36, a Strain Isolated from a Patient with an Upper Urinary Tract Infection“. Genome Announcements 4, Nr. 6 (15.12.2016). http://dx.doi.org/10.1128/genomea.01403-16.

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Here, we announce the complete genome sequence of Klebsiella pneumoniae KP36, a strain isolated from a patient with a severe community-acquired urinary tract infection. This genome provides insights into the pathogenesis of a pandemic K. pneumoniae strain from a community-acquired urinary tract infection.

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