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Auswahl der wissenschaftlichen Literatur zum Thema „I-B. Abortus“
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Zeitschriftenartikel zum Thema "I-B. Abortus"
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El-Boshy, M., H. Abbas, S. El-Khodery und S. Osman. „Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius)“. Veterinární Medicína 54, No. 1 (11.02.2009): 25–32. http://dx.doi.org/10.17221/3044-vetmed.
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The present study had the aim of assessing the cytokine response and selected clinicopathological findings associated with brucellosis in camels (<I>Camelus dromedarius)</I>. 340 dromedary camels were examined for brucellosis using agglutination and Complement Fixation tests (CFT). Twenty-five camels (7.35%) were positive by both tests; 14 (4.12%) for <I>B. abortus</I> and 11 (3.23%) for <I>B. melitensis</I>. IL-1β and IL-10 interleukin levels in both <I>B. abortus</I> and <I>B. melitensis</I> infected camels showed significant elevations (<I>P</I> < 0.05) compared with controls. Moreover, there was significantly larger increase in IL-1β interleukins in camels infected with <I>B. abortus</I> compared with <I>B. melitensis</I>. TNF-α, IFN-γ and IL-1α levels showed significant decreases (<I>P</I> < 0.05) in <I>Brucella</I> infected camels compared with non-infected ones; however, there was non-significant changes in IL-6 levels in <I>Brucella</I> infected camels compared with controls. Lymphopenia was recorded in infected camels but not in controls. However, normocytic normochromic anemia, hypoproteinemia, hypoalbuminemia and hypoglycemia were recorded in the <I>B. abortus</I> group only. Sorbitol dehydrogenase (SD), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) showed significant increases (<I>P</I> < 0.05) in infected camels compared with controls, and in <I>B. abortus</I> infected camels compared with <I>B. melitensis</I> infected animals. This is the first report that describes changes in selected cytokines and various haematological and biochemical parameters associated with brucellosios in dromedary camels. Emphasis should be placed on multidisciplinary research to elucidate the immunomodulatory features of camel brucellosis.
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Arriola Benitez, Paula Constanza, Ayelén Ivana Pesce Viglietti, María Mercedes Elizalde, Guillermo Hernán Giambartolomei, Jorge Fabián Quarleri und María Victoria Delpino. „Hepatic Stellate Cells and Hepatocytes as Liver Antigen-Presenting Cells during B. abortus Infection“. Pathogens 9, Nr. 7 (30.06.2020): 527. http://dx.doi.org/10.3390/pathogens9070527.
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In Brucellosis, the role of hepatic stellate cells (HSCs) in the induction of liver fibrosis has been elucidated recently. Here, we study how the infection modulates the antigen-presenting capacity of LX-2 cells. Brucella abortus infection induces the upregulation of class II transactivator protein (CIITA) with concomitant MHC-I and -II expression in LX-2 cells in a manner that is independent from the expression of the type 4 secretion system (T4SS). In concordance, B. abortus infection increases the phagocytic ability of LX-2 cells and induces MHC-II-restricted antigen processing and presentation. In view of the ability of B. abortus-infected LX-2 cells to produce monocyte-attracting factors, we tested the capacity of culture supernatants from B. abortus-infected monocytes on MHC-I and –II expression in LX-2 cells. Culture supernatants from B. abortus-infected monocytes do not induce MHC-I and -II expression. However, these supernatants inhibit MHC-II expression induced by IFN-γ in an IL-10 dependent mechanism. Since hepatocytes constitute the most abundant epithelial cell in the liver, experiments were conducted to determine the contribution of these cells in antigen presentation in the context of B. abortus infection. Our results indicated that B. abortus-infected hepatocytes have an increased MHC-I expression, but MHC-II levels remain at basal levels. Overall, B. abortus infection induces MHC-I and -II expression in LX-2 cells, increasing the antigen presentation. Nevertheless, this response could be modulated by resident or infiltrating monocytes/macrophages.
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Balakhonov, S. V., V. I. Dubrovina, V. V. Voitkova, K. M. Korytov, N. L. Barannikova, V. B. Nikolaev und T. T. Shkaruba. „Immunophenotyping of blood cells of experimental animals immunized with Brucella abortus thermoextracts“. Journal of microbiology epidemiology immunobiology, Nr. 4 (02.09.2019): 25–31. http://dx.doi.org/10.36233/0372-9311-2019-4-25-31.
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Aim. To study the subpopulational structure of blood cells of the experimental animals immunized with thermoextracts (TE) of Brucella abortus in L- and S-form. Materials and methods. Total 100 certified («Vector», Novosibirsk) outbred mice were immunized with B. abortus I-206 TE in L- and S-form in 20 μg protein dose. After 1, 3, 7, 14 and 21 days of observation the phenotypes (CD45, CD3, CD4, CD8, CD19, CD69) of blood cells were detected. Results. General regularities were revealed after injection of the experimental preparations. So, B. abortus TE in L- and S-form caused the immune response that increased granulocyte number and expression of early activation marker CD69 by T- and B-lymphocytes of blood in early period of observation (1-3 days), decrease in general B-lymphocyte content in late periods of observation (7-21 days). Thus, mice received B. abortus ТE in L-form demonstrated authentically higher CD69 expression of blood lymphocyte subpopulations than mice received B. abortus ТE in S-form. Distinctions in formation of humoral immune response were revealed that probably was connected with alteration of Brucella chemical composition in the course of L-transformation. Conclusion. The investigation established that B. abortus TE in L- or S-form caused immunological reorganization in the experimental animal organisms. On the basis of the fin
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Astarina, Dhevie Kenny, Eko Sugeng Pribadi und Fachriyan Hasmi Pasaribu. „Penggunaan Imunostik sebagai Uji Serologi untuk Deteksi Brucella abortus pada Sapi (APPLICATION IMMUNOSTICK ASSAY FOR SEROLOGICAL TEST BRUCELLA ABORTUS IN BOVINE)“. Jurnal Veteriner 19, Nr. 2 (31.07.2018): 169. http://dx.doi.org/10.19087/jveteriner.2018.19.2.169.
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Serological test is one of diagnostic method to detect pathogenicity brucella. Several methods are being improving such as Rose Bengal Test (RBT), Complement Fixation Test (CFT) dan Enzyme-linked Immunosorbent Assay (ELISA). Immunostick has an accuracy equivalent to ELISA and is easy to apply in the field so it is possible to be applied as a rapid test for brucellosis detection. The study aim was to know sensitivity and specificity of immunostick that were used to detecte antibody Brucella abortus using commercial antigens of B. abortus Strain 19 (S19) and B. abortus Strain 99 (S99). The test have compared with ELISA. The tests were conducted in two stages, namely (i) immunostick ability to detect antibodies in seropositive and seronegative serum, and (ii) the immunostick result were compared with ELISA result in serum grup that were be know and unknown status. A total of 250 serums were examined and result indicated that immunostick can be detect B. abortus antibodies in cattle serum with sensitivity 100%. Immunostick specifity were 45,45% for B. abortus S99(1) antigen; 78,79% for B. abortus S99(2) antigen and 51,52% for B. abortus S19 antigen. When the test compared with ELISA, the sensitivity 82,86% and the spesifity were 52,31% for B. abortus S99(1) antigen; 93,54% and 79,71 for B. abortus S99(2) antigen and 82,86% and 58,46% for B. abortus S19 antigen.
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Gee, Jason M., Michelle Wright Valderas, Michael E. Kovach, Vanessa K. Grippe, Gregory T. Robertson, Wai-Leung Ng, John M. Richardson, Malcolm E. Winkler und R. Martin Roop. „The Brucella abortus Cu,Zn Superoxide Dismutase Is Required for Optimal Resistance to Oxidative Killing by Murine Macrophages and Wild-Type Virulence in Experimentally Infected Mice“. Infection and Immunity 73, Nr. 5 (Mai 2005): 2873–80. http://dx.doi.org/10.1128/iai.73.5.2873-2880.2005.
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ABSTRACT Two-dimensional gel electrophoretic analysis of cell lysates from Brucella abortus 2308 and the isogenic hfq mutant Hfq3 revealed that the RNA binding protein Hfq (also known as host factor I or HF-I) is required for the optimal stationary phase production of the periplasmic Cu,Zn superoxide dismutase SodC. An isogenic sodC mutant, designated MEK2, was constructed from B. abortus 2308 by gene replacement, and the sodC mutant exhibited much greater susceptibility to killing by O2 − generated by pyrogallol and the xanthine oxidase reaction than the parental 2308 strain supporting a role for SodC in protecting this bacterium from O2 − of exogenous origin. The B. abortus sodC mutant was also found to be much more sensitive to killing by cultured resident peritoneal macrophages from C57BL6J mice than 2308, and the attenuation displayed by MEK2 in cultured murine macrophages was enhanced when these phagocytes were treated with gamma interferon (IFN-γ). The attenuation displayed by the B. abortus sodC mutant in both resting and IFN-γ-activated macrophages was alleviated, however, when these host cells were treated with the NADPH oxidase inhibitor apocynin. Consistent with its increased susceptibility to killing by cultured murine macrophages, the B. abortus sodC mutant also displayed significant attenuation in experimentally infected C57BL6J mice compared to the parental strain. These experimental findings indicate that SodC protects B. abortus 2308 from the respiratory burst of host macrophages. They also suggest that reduced SodC levels may contribute to the attenuation displayed by the B. abortus hfq mutant Hfq3 in the mouse model.
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Van Lent, Sarah, Heather Huot Creasy, Garry S. A. Myers und Daisy Vanrompay. „The Number, Organization, and Size of Polymorphic Membrane Protein Coding Sequences as well as the Most Conserved Pmp Protein Differ within and across Chlamydia Species“. Journal of Molecular Microbiology and Biotechnology 26, Nr. 5 (2016): 333–44. http://dx.doi.org/10.1159/000447092.
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Variation is a central trait of the polymorphic membrane protein (Pmp) family. The number of <i>pmp</i> coding sequences differs between <i>Chlamydia</i> species, but it is unknown whether the number of <i>pmp</i> coding sequences is constant within a <i>Chlamydia</i> species. The level of conservation of the Pmp proteins has previously only been determined for <i>Chlamydia trachomatis.</i> As different Pmp proteins might be indispensible for the pathogenesis of different <i>Chlamydia </i>species, this study investigated the conservation of Pmp proteins both within and across <i>C. trachomatis,</i><i>C. pneumoniae,</i><i>C. abortus,</i> and <i>C. psittaci.</i> The <i>pmp</i> coding sequences were annotated in 16 <i>C. trachomatis,</i> 6 <i>C. pneumoniae,</i> 2 <i>C. abortus,</i> and 16 <i>C. psittaci</i> genomes. The number and organization of polymorphic membrane coding sequences differed within and across the analyzed <i>Chlamydia </i>species. The length of coding sequences of <i>pmpA,</i><i>pmpB,</i> and <i>pmpH</i> was conserved among all analyzed genomes, while the length of <i>pmpE/F</i> and <i>pmpG,</i> and remarkably also of the subtype <i>pmpD,</i> differed among the analyzed genomes. PmpD, PmpA, PmpH, and PmpA were the most conserved Pmp in <i>C. trachomatis,</i><i>C. pneumoniae,</i><i>C. abortus,</i> and <i>C. psittaci</i>, respectively. PmpB was the most conserved Pmp across the 4 analyzed <i>Chlamydia</i> species.
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Mikhailov, L. M., N. L. Barannikova, L. E. Tokareva, S. A. Vityazeva, T. P. Starovoytova, V. I. Dubrovina und S. V. Balakhonov. „Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs“. Epidemiology and Vaccine Prevention 15, Nr. 4 (20.08.2016): 82–86. http://dx.doi.org/10.31631/2073-3046-2016-15-4-82-86.
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Relevance. In the Russian Federation is noted the negative dynamics of epizootic process of brucellosis among epidemiologically important species of farm animals (cattle and small ruminants), which represents a threat to the population. Used in Russia live vaccine based on a strain of Brucella abortus 19 the BA has reduced virulence, but capable at high doses (108 -2 109 m.c.) cause a generalized infection in guinea pigs and humans, and in violation of the rules cause post-vaccination complications. Goal. Assess possibility of the thermo-extract derived from the S- and L-forms of Brucella, get an immune response in guinea pigs, and reduce the risk of infection with virulent brucella. Materials and methods. Two series of experiments were carried out on guinea pigs. Immunized guinea pigs thermo-extracts (TE) from a strain of B. abortus I-206 in the S- and L-forms, and live brucellosis vaccine (Scientific and Production Association for Immunological Preparations «Microgen», Russia). To infect guinea pigs using virulent B. abortus 544 (Reference) and B. melitensis I-203 from the museum of living cultures of the Irkutsk Scientific Research Anti-Plague Institute. Results. In the first and second experiment after immunization L TE in dose 5 mg and 10 mg after infection with B. abortus and B. melitensis 5441-203 were approximately similar results. Immunization of Brucella in TE S-form or complex of L + S TE either of two doses (5 mg or 10 mg) cultures after infection B. abortus and B. melitensis 5441-203 gave the same result as a vaccine B. abortus 19VA. Conclusions. The results indicate the prospects of further study of experimental steps for using immunizing agents TE S, L TE and TE S + L on the laboratory animals.
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Velasco, J., J. A. Bengoechea, K. Brandenburg, B. Lindner, U. Seydel, D. González, U. Zähringer, E. Moreno und I. Moriyón. „Brucella abortus and Its Closest Phylogenetic Relative, Ochrobactrum spp., Differ in Outer Membrane Permeability and Cationic Peptide Resistance“. Infection and Immunity 68, Nr. 6 (01.06.2000): 3210–18. http://dx.doi.org/10.1128/iai.68.6.3210-3218.2000.
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ABSTRACT The outer membrane (OM) of the intracellular parasiteBrucella abortus is permeable to hydrophobic probes and resistant to destabilization by polycationic peptides and EDTA. The significance of these unusual properties was investigated in a comparative study with the opportunistic pathogens of the genusOchrobactrum, the closest known Brucellarelative. Ochrobactrum spp. OMs were impermeable to hydrophobic probes and sensitive to polymyxin B but resistant to EDTA. These properties were traced to lipopolysaccharide (LPS) because (i) insertion of B. abortus LPS, but not of Escherichia coli LPS, into Ochrobactrum OM increased its permeability; (ii) permeability and polymyxin B binding measured with LPS aggregates paralleled the results with live bacteria; and (iii) the predicted intermediate results were obtained with B. abortus-Ochrobactrum anthropi and E. coli-O. anthropiLPS hybrid aggregates. Although Ochrobactrum was sensitive to polymyxin, self-promoted uptake and bacterial lysis occurred without OM morphological changes, suggesting an unusual OM structural rigidity.Ochrobactrum and B. abortus LPSs showed no differences in phosphate, qualitative fatty acid composition, or acyl chain fluidity. However, Ochrobactrum LPS, but not B. abortus LPS, contained galacturonic acid. B. abortusand Ochrobactrum smooth LPS aggregates had similar size and zeta potential (−12 to −15 mV). Upon saturation with polymyxin, zeta potential became positive (1 mV) for Ochrobactrum smooth LPS while remaining negative (−5 mV) for B. abortus smooth LPS, suggesting hindered access to inner targets. These results show that although Ochrobactrum and Brucella share a basic OM pattern, subtle modifications in LPS core cause markedly different OM properties, possibly reflecting the adaptive evolution ofB. abortus to pathogenicity.
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Seco-Mediavilla, Patricia, Jean-Michel Verger, Maggy Grayon, Axel Cloeckaert, Clara M. Marín, Michel S. Zygmunt, Luis Fernández-Lago und Nieves Vizcaíno. „Epitope Mapping of the Brucella melitensis BP26 Immunogenic Protein: Usefulness for Diagnosis of Sheep Brucellosis“. Clinical Diagnostic Laboratory Immunology 10, Nr. 4 (Juli 2003): 647–51. http://dx.doi.org/10.1128/cdli.10.4.647-651.2003.
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ABSTRACT Sequencing of bp26, the gene encoding the Brucella sp. immunogenic BP26 periplasmic protein, was performed in the reference strains of Brucella abortus, B. suis, and B. ovis. The three bp26 sequences were almost identical to that published for B. melitensis 16M bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. The bp26 genes of the seven B. abortus biovar reference strains and B. abortus S19 and RB51 vaccine strains were also sequenced. Again, only minor differences were found. Surprisingly, the bp26 nucleotide sequence for B. abortus S19 was almost identical to that found for B. melitensis 16M and differed from the sequence described previously by others (O. L. Rossetti, A. I. Arese, M. L. Boschiroli, and S. L. Cravero, J. Clin. Microbiol. 34:165-169, 1996) for the same B. abortus strain. The epitope mapping of BP26, performed by using a panel of monoclonal antibodies and recombinant DNA techniques, allowed the identification of an immunodominant region of the protein interesting for the diagnosis of B. melitensis and B. ovis infection in sheep. A recombinant fusion protein containing this region of BP26 reacted indeed, in Western blotting, as the entire recombinant BP26 against sera from B. melitensis- or B. ovis-infected sheep while it avoided false-positive reactions observed with sera from Brucella-free sheep when using the entire recombinant BP26. Thus, use of this recombinant fusion protein instead the entire recombinant BP26 could improve the specific serological diagnosis of B. melitensis or B. ovis infection in sheep.
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Ciocchini, Andrés E., Mara S. Roset, Nora Iñón de Iannino und Rodolfo A. Ugalde. „Membrane Topology Analysis of Cyclic Glucan Synthase, a Virulence Determinant of Brucella abortus“. Journal of Bacteriology 186, Nr. 21 (01.11.2004): 7205–13. http://dx.doi.org/10.1128/jb.186.21.7205-7213.2004.
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ABSTRACT Brucella abortus cyclic glucan synthase (Cgs) is a 316-kDa (2,831-amino-acid) integral inner membrane protein that is responsible for the synthesis of cyclic β-1,2-glucan by a novel mechanism in which the enzyme itself acts as a protein intermediate. B. abortus Cgs uses UDP-glucose as a sugar donor and has the three enzymatic activities necessary for synthesis of the cyclic polysaccharide (i.e., initiation, elongation, and cyclization). Cyclic glucan is required in B. abortus for effective host interaction and complete expression of virulence. To gain further insight into the structure and mechanism of action of B. abortus Cgs, we studied the membrane topology of the protein using a combination of in silico predictions, a genetic approach involving the construction of fusions between the cgs gene and the genes encoding alkaline phosphatase (phoA) and β-galactosidase (lacZ), and site-directed chemical labeling of lysine residues. We found that B. abortus Cgs is a polytopic membrane protein with the amino and carboxyl termini located in the cytoplasm and with six transmembrane segments, transmembrane segments I (residues 419 to 441), II (residues 452 to 474), III (residues 819 to 841), IV (residues 847 to 869), V (residues 939 to 961), and VI (residues 968 to 990). The six transmembrane segments determine four large cytoplasmic domains and three very small periplasmic regions.
Dissertationen zum Thema "I-B. Abortus"
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Skosana, Banele Irene. „Validation of the Fluorescence Polarization Assay (FPA) for the diagnosis of Bovine Brucellosis“. Diss., 2021. http://hdl.handle.net/10500/27541.
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Abstracts in English and ZuluFluorescence polarization assay (FPA), a serological assay, was validated as an alternative test for the rapid and cost-effective diagnosis of bovine brucellosis, with the aim of improving the control of brucellosis in South Africa. The FPA is anticipated to distinguish between vaccinated and infected cattle, circumventing the challenge associated with the tests that are currently used. Positive cattle serum samples (n =420) confirmed by Complement Fixation Test were tested in conjunction with serum samples (n = 446) from non-infected cattle initially tested on Rose Bengal Test, CFT and compared with FPA. The optimum cut-off value that offers the highest diagnostic sensitivity (Dsn) and diagnostic specificity (Dsp) was determined as 87 mP with the use of ROC analysis. The Dsn and Dsp of FPA using this cut-off value was calculated at 99.09% - 100% and 68.09%- 76.61% respectively with a 95% confidence interval (cl). The area under curve (AUC) was calculated at 0.9842 with a 95% standard error (S.E) of 0.005532 with positive and negative likelihood ratio (+LR) (-LR) at 3.643 and 1.002, respectively. The FPA was found to be as effective as CFT and should be considered because of its accuracy and other advantages such as speed, high throughput and the objectivity of the interpretation of results that can be obtained electronically by the (PHERAstar) machine. The test should be included in routine serological diagnosis for brucellosis.
I- Fluorescence polarization assay (i-FPA) ukuhlolwa kwe-serological okuqinisekiswe njengenye indlela yokuhlola ukuxilongwa okusheshayo nengabizi kwe-bovine brucellosis, okuzokwenza ngcono ukulawulwa kwe-brucellosis eNingizimu Afrika. Ngaphezu kwalokho, i-FPA kulindeleke ukuthi yehlukanise phakathi kwezinkomo ezigonyiwe nezithelelekile futhi lokhu kuzonciphisa inselelo ehambisana nokuhlolwa esetshenziswa njengamanje. Amasampula amahle avumayo we-serum ezinkomo (n = 420) aqinisekiswa yi-CFT ahlolwe ngokuhlangana namasampula e-serum (n = 446) avela ezinkomeni ezingathelelekile ezahlolwa kuqala ku-RBT, CFT futhi kuqhathaniswa ne-FPA. Inani elinqunyiwe elikhulu elinikezela ukuzwela okuphezulu kokuxilonga (i-Dsn) kanye nokucaciswa kokuxilongwa (i-Dsp) kunqunywe njenge-87 mP kusetshenziswa ukuhlaziywa kwe-ROC. I-Dsn ne-Dsp ye-FPA esebenzisa leli nani elisikiwe libalwe ngama-99.09% - 100% no-68.09% - 76.61% ngokulandelana kwesikhathi sokuzethemba esingu-95% (cl). Indawo engaphansi kwe-Curve noma ijika thizeni (i-AUC) ibalwe ku-0.9842 enephutha elingu-95% elijwayelekile (SE) lika- 0.005532 elinezilinganiso ezinhle nezimbi ze-likehood (+ LR) (-LR) ngo-3.643 no- 1.002, ngokulandelana. I-FPA isebenza njenge-CFT futhi kufanele ibhekwe ngenxa yokunemba eneqiniso kwayo nezinye izinzuzo ezifana nejubane lokuthola imiphumela kanye nenhloso yokuchazwa kwemiphumela engatholakala ngomshini wekhomphuyitha (PHERAstar), i-FPA kufanele ifakwe ekuhlolweni okuvamile ngokujwayelekile kwe-serological ye-brucellosis.
Agriculture and Animal Health
M. Sc. (Agriculture)