Zeitschriftenartikel zum Thema „Hub dyno“

In order to analyse the rock breakage process of double-edge ball tooth hob cutter and get the related curve of intrusive force and invasion depth, the numerical simulation model of double-edge ball tooth hob cutter is accurately established. In the process of interaction between hob cutter and rock mass, the material model is also established, the mechanism of rock fragmentation is carefully researched, the elastic-plastic deformation is seriously studied. Finally, we design an experiment which has a good consistency with the simulation analysis.

According to principle of hobbing, this paper set up accurate model of gear processed by full radius hob based on ANSYS/LS-DYNA software. A dynamic contact analysis of gears are completed and we gets stress distribution of tooth root as time changes, making foundation for future study and optimization design of full radius hob.

Adenovirus type 2 (Ad2) binds the coxsackie B virus Ad receptor and is endocytosed upon activation of the αv integrin coreceptors. Here, we demonstrate that expression of dominant negative clathrin hub, eps15, or K44A-dynamin (dyn) inhibited Ad2 uptake into epithelial cells, indicating clathrin-dependent viral endocytosis. Surprisingly, Ad strongly stimulated the endocytic uptake of fluid phase tracers, coincident with virus internalization but without affecting receptor-mediated transferrin uptake. A large amount of the stimulated endocytic activity was macropinocytosis. Macropinocytosis depended on αv integrins, PKC, F-actin, and the amiloride-sensitive Na+/H+ exchanger, which are all required for Ad escape from endosomes and infection. Macropinocytosis stimulation was not a consequence of viral escape, since it occurred in K44A-dyn–expressing cells. Surprisingly, 30–50% of the endosomal contents were released into the cytosol of control and also K44A-dyn–expressing cells, and the number of fluid phase–positive endosomes dropped below the levels of noninfected cells, indicating macropinosomal lysis. The release of macropinosomal contents was Ad dose dependent, but the presence of Ad particles on macropinosomal membranes was not sufficient for contents release. We conclude that Ad signaling from the cell surface controls the induction of macropinosome formation and leakage, and this correlates with viral exit to the cytosol and infection.

Research on the parameters of full blood count and differential white blood count is included in the program of all medical laboratories of primary, secondary and tertiary health care levels. Today, all haematological tests are exclusively performed on the haematology analyzers. Automation of haematology laboratories is a result of the huge requires for haematological test performing, timely issuing of the haematological findings, and possibility of the usage of modern techniques.This work is an evaluation of laser haematology analyzer Cell-Dyn 3700 SL. It investigates the reliability of test results throughout the following parameters: precision, accuracy, sensitivity and specificity of determination methods. It also explores the influence of sample transferring and correlation with haematology analyzer MAXM Retti. Haematology parameters that have been investigated are: white blood cell (WBC), neutrophils (NEU), lymphocytes (LXM), monocytes (MONO), eosinophils (EOS), basophils (BASO), red blood cells (RBC), haemoglobin (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCHC) red cell distribution width (RDW), platelet (PLT), mean platelet volume (MPV), plateletocrit (PCT), and platelet distribution width (PDW).The results confirms that precision of analyzer fulfils the reproducibility of testing parameters: WBC, RBC, HGB, MCV, MCH, MCHC, and PLT. Correlation coefficient values (r) gained throughout the statistical analysis, that is linear regression results obtained throughout the comparison of two analyzers are adequate except for MCHC (r = 0.64), what is in accordance with literature data.Accuracy is tested by haematology analyzer method and microscopic differentiating method. Correlation coefficient results for granulocytes, lymphocytes and monocytes point the accuracy of methods. Sensitivity and specificity parameters fulfil the analytical criteria.It is confirmed that haematology analyzer Cell-Dyn 3700 SL is reliable for the determination of full blood count in everyday work. Analyzer and its program for differential white blood count can be used for the research and separation of normal and pathological blood counts with addition of microscopic methods confirming distribution or morphologic changes of leukocytes.

The objective of this study was to evaluate the influence of time of sample storage, method of analysis, and storage temperature on stability of HCT, HGB, and RBC in avian blood samples. Blood samples from mute swans were stored at 24°C or 4°C. Analyses of HCT, HGB, and RBC were carried out after 5 and 25 hours after collection of blood using both traditional and Cell-Dyn 3700 analyzer methods. Storage temperature had no significant influence on HCT, HGB, and RBC values of mute swan blood. However hematological parameters obtained by the traditional method were significantly lower than the results from the analyzer. Time of storage had a significant influence on values obtained traditionally for HCT, HGB, and RBC, but did not affect results that were obtained by the multi-parameter automated method. These results indicate that the mute swan blood can be stored at both room and refrigerator temperature. However, the analysis should be performed as soon as possible, especially in the case of traditional methods. Reference intervals for this species should always include information about the method used for hemogram determination and time elapsed since the blood collection.

Sodium lignosulphonate (SLS) is a product of isolated lignin sulphonation process using NaHSO3 as sulphonation agent.The research begins with isolated lignin from palm shell amd continued with sulphonation with mass ratio of palm shell lignin – NaHSO3(b/b) of 1:0.2, 1:0.3, and 1:0.5 and initial pH of 6, 7, and 8 at temperature 85 oC and 95 oC. The purity of SLS made in this studies was 66,782 %. The CMC value of this SLS ranged from 0,014 – 0,052; while the lowering of surface tension of water ranged from 53,63 – 59,83 dyne/cm. The HLB value ranged from 3,805 – 4,278; means that this surfactant classified as lipophilic. The resulted SLS was dark brown, and water soluble.

AbstractBackgroundBlood counters are primarily used to measure peripheral blood cells including platelets (PLTs). In routine quality control of platelet concentrates (PCs), counters are also used to analyze very high PLT counts. To meet the requirements of national and European guidelines for quality assurance, the accuracy of counting very high PLT counts has to be validated. The aim of the present study was to validate four blood counters (one of which has two detection methods) focusing on the PLT count.MethodsThe comparison was performed with PCs using the blood counter devices CELL-DYN Ruby (optical count) and CELL-DYN Emerald (impedance count), Sysmex K-4500 (impedance count), Sysmex XN-550 (impedance count) and Sysmex XN-550 (optical count). For precision performances, samples were measured serially 5 times and the coefficients of variation were calculated and compared with manufacturers’ requirements. Additionally, 50 peripheral blood samples were analyzed and standard hemogram parameters (red blood cells [RBC], white blood cells [WBC], hemoglobin [HGB], hematocrit [HCT], PLTs) were compared.ResultsThe comparison showed significant differences between the studied blood counter devices in measuring high PLT counts. The CELL-DYN-Emerald, the Sysmex K-4500 and Sysmex XN-500 with the optical counting method measured significantly higher PLT counts compared to the CELL-DYN-Ruby and the Sysmex XN-500 with the impedance counting technology (p<0.0001) independent of their principle of measurement. The manufacturers provide comparable coefficients of variation. We achieved similar results for all counters. All results of the peripheral blood count parameters were comparable.ConclusionsOur study showed the importance of blood counter validations focusing on PCs with high PLT counts before routine use. Not only the generally fundamental method, but also the manufacturers’ peculiarities seem to play an important role.

Complete blood count (CBC) is one of the laboratory tests most often influenced by doctors. The use of a hematology analyzer offers a wider range of probe parameters. The pre-analytic stage accounts for 70% of errors, one of which is the delay of the examination. Changes in report results were reported due to changes in blood cell morphology due to EDTA additives and room temperature. The aim of this research is the disturbance of stability of the results of examination of various CBC parameters in blood samples that are left at room temperature for 24 hours using a hematology analyzer. This experimental laboratory research was conducted at the Pramita Jemur Andayani Clinical Laboratory. Blood samples were obtained from volunteers, stored at room temperature and subjected to immediate examination (control) and after a delay of 6, 12 and 24 hours (treatment). A total of 30 respondents, consisting of 8 men and 22 women. The mean age of the respondents was 22 ± 1 year. There was no difference in the results of the examination (p-value > 0.05) in the RBC, Hct MCV, MCHC, PLT and PDW. The results of the examination (p-value < 0.05) were found on Hgb, MCH, RDW, WBC, NEU, IG, MONO, EO, BASO, LYM, PLT and PDW. Delayed CBC examinations using the CELL-DYN Ruby hematology analyzer directly gave different results on several parameters ranging from 6 hours delay of examination.

The article presents the design of a drum furnace, providing for the provision of differentiated heat flows through the use of an internal perforated pipe. The design of the presented horizontal drum dryer with a perforated tube having openings is designed to eliminate such technological risk factors as the ignition of the oil component of the processed material, and the metal dispersed material itself having pyrophoric properties. As a result of the numerical simulation using the LS–DYNA program, the temperature and heat fluxes in the quasi-boiling zone of the drained oil-containing dispersed material were determined. The calculations made it possible to establish the dependence of the temperature in the quasi-boiling zone on the number of holes of the perforated pipe. Analysis of the obtained graphs allows us to conclude that the gradient of temperature (heat) flows in fixed sections of the furnace of the quasi-boiling zone.

The article presents the design of a drum furnace, providing for the provision of differentiated heat flows through the use of an internal perforated pipe. The design of the presented horizontal drum dryer with a perforated tube having openings is designed to eliminate such technological risk factors as the ignition of the oil component of the processed material, and the metal dispersed material itself having pyrophoric properties. As a result of the numerical simulation using the LS–DYNA program, the temperature and heat fluxes in the quasi-boiling zone of the drained oil-containing dispersed material were determined. The calculations made it possible to establish the dependence of the temperature in the quasi-boiling zone on the number of holes of the perforated pipe. Analysis of the obtained graphs allows us to conclude that the gradient of temperature (heat) flows in fixed sections of the furnace of the quasi-boiling zone.

Abstract Sickle Cell Disease (SCD) is an inherited disorder of the β-globin chain of hemoglobin, in which a single point mutation leads to decreased deformability of red blood cells (RBCs) and increased cellular adhesion to endothelium. The effect of this mutation on RBCs has been well characterized, and the interplay of endothelial cells, RBCs, and white blood cells (WBCs) have also been well characterized. However, few studies have specifically investigated how platelets interact with endothelial cells and other blood cells in the context of SCD and the role these cell fragments may have in vaso-occlusion. To that end, we utilized microfluidic technology previously developed in our lab to perform a “real time” in vitro analyses of platelet-endothelial cell interactions in SCD patient samples. This “microvasculature-on-a-chip” enables the visualization of blood cell-endothelial cell interactions under a controlled hemodynamic environment (Tsai et al, JCI, 2012). As shear stress can trigger platelet activation, we further modified and optimized our standard microfluidic devices to encompass 3 different physiologic shear rates. Our device features microchannels 50µm in diameter with human umbilical vein endothelial cells (HUVEC) confluently lining the channels; there are 12 channels in each device, grouped in 3 sets of 4 channels with graduating shear rates spanning 3 orders of magnitude (Figure 1). Our initial experiments were performed under normoxic conditions allowing characterization of platelet-endothelial interactions in an “arterial” in vitro environment. Whole blood samples were obtained from 3 patient populations: patients with HgbSS SCD on hydroxyurea (HgbSS+HU), patients with HgbSS SCD not on hydroxyurea (HgbSS-no HU), and normal healthy controls. Over 30 minutes, whole blood stained with fluorescently labeled CD41 to identify platelets and Hoeschst to identify HUVEC nuclei was perfused at a rate of 1.5µl/minute under videomicroscopy. Accumulation of platelets on the endothelialized channels and platelet aggregates were quantified based on anti-CD41 fluorescence. Within 1 minute of perfusion, HgbSS-no HU whole blood samples exhibited extensive platelet aggregates at 1 and 10 dyne/cm2 (Figure 2); this phenomenon did not occur under any of the shear conditions in blood samples from Hgb SS+HU or healthy control samples. In HgbSS-no HU blood samples, some of these “thrombi”-like aggregates were stable under flow, increased in size, and persisted for the remainder of the 30 minute experiments. In contrast, mild, uniform, platelet adhesion slowly developed at high shear conditions in Hgb SS+HU with fewer platelet aggregates forming as compared to patients with HgbSS- no HU. Healthy control samples did not exhibit this platelet aggregation. There appears to be an attenuating effect of hydroxyurea on platelets that prevents platelet clumping from occuring as frequently under various shear conditions that is not present in the Hgb SS-no HU samples (Figure 3). In conclusion, using our novel in vitro system, we have demonstrated the platelets from Hgb SS-no HU patients have a significantly increased propensity to adhere, aggregate, and accumulate in endothelialized microvasculature-sized microchannels. Interestingly, this effect appears to be attenuated in blood samples from Hgb SS+HU patients and not present in healthy controls, demonstrating that hydroxyurea appears to be an important modifier of this phenomenon. Experiments investigating the underlying mechanisms of this phenomenon, the effects of deoxygenation and the potential role of platelets in vaso-occlusion, the effects of sickle cell platelet adhesion/aggregation on endothelial function, and how hydroxyurea may or may not affect any or all of these parameters, are all currently ongoing. Figure 1 Figure 1. Figure 2 Figure 2. Figure 3 Figure 3. Disclosures No relevant conflicts of interest to declare.

Abstract Introduction Several studies have shown slow wave sleep (SWS) is altered with ageing. However, most of these studies have been conducted in-lab and usually over a single night. In this study, we assessed the evolution of process S with ageing by analysing the dynamics of endogenous and auditory-evoked slow waves in a large population. Methods 300 participants (200 M, 20 - 70 y.o.) were selected from volunteers users wearing a sleep headband for at least 3 nights, meeting the criteria of high signal quality and having no subjective sleep complaints nor being shift-workers. The Dreem headband is a connected device able to monitor EEG signals as well as pulse and movement and performs sleep staging in real-time automatically. Slow waves were detected as large negative deflections on the filtered EEG signals during NREM sleep. The auditory evoked slow waves were done using a previously validated closed-loop procedure. Results In our study, age was strongly correlated with N3 sleep duration (r=-0.34, p&lt;0.0001), slow wave amplitude (r=-0.25, p&lt;0.0001), and slow wave density (r=-0.40, p&lt;0.0001). The slope of the slow wave activity, representing the process S here, was significantly decreased (r=-0.32, p&lt;0.0001). This effect was mainly due to changes in the density of slow waves in the first 2 hours of sleep (r=-0.41, p&lt;0.0001). Finally, our results show a decrease in the probability of auditory evoked slow waves (r=-0.43, p&lt;0.0001). Conclusion These results confirmed the in-lab studies showing a heterogeneous alteration of homoeostatic process S with age, as well as a general decrease of slow wave occurrences, that is observed in parallel of a decrease of the probability of evoking slow waves, suggesting a global change in the system responsible for slow wave generation. Support This study was supported by Dreem sas and ANR, FLAG ERA 2015, HPB SLOW-Dyn

Background. Hematological abnormalities are common in pulmonary tuberculosis (PTB) patients, which is one of the major public health problems worldwide. However, there is paucity of information about the hematological profile of PTB patients with and without HIV in the study area. Therefore, this study aimed to assess hematological abnormalities of pulmonary tuberculosis patients with and without HIV at the University of Gondar Hospital, Northwest Ethiopia. Methods. A comparative cross-sectional study was conducted at the University of Gondar Hospital. Sociodemographic data was collected using a pretested, structured questionnaire. Five milliliters of venous blood sample was collected and divided into a 3 ml EDTA tube for complete blood count with the Cell Dyn 1800 hematological analyzer and a 2 ml citrated tube for erythrocyte sedimentation rate determination. Data were entered into Epi Info version 3.5.3 and then transferred to SPSS 20 for analysis. The independent samples t-test was used to compare the mean values of hematological parameters between PTB patients and PTB-HIV coinfected patients. Result. A total of 100 study subjects (50 PTB and 50 PTB-HIV coinfected) were included with a mean age of 31.3 ± 10.3 years for PTB patients and 32.1 ± 9.2 years for PTB-HIV coinfected patients. In this study, there were significantly lower mean values of Hgb (P = 0.049), platelet count (P < 0.001), and neutrophils counts (P = 0.007) among PTB-HIV coinfected patients when compared with PTB patients. Of the PTB infected patients 46% were anemic, 6% leukopenic, 22% neutropenic, 8% lymphopenic, and 8% thrombocytopenic. On the other hand, of the PTB-HIV coinfected patients 60% were anemic, 14% leukopenic, 66% neutropenic, 12% lymphopenic, and 20% thrombocytopenic. ESR value was increased in all patients. Conclusion. This study demonstrated high prevalence of neutropenia, anemia, and thrombocytopenia among PTB-HIV coinfected patients. HIV coinfection worsens hematological abnormalities of PTB patients. Assessment of hematological parameters can be used as an indicator in the diagnosis and follow-up of PTB patients coinfected with HIV. We recommended assessment of PTB patients with or without HIV for various hematological disorders such as neutropenia, anemia, and thrombocytopenia.

Abstract Background Recent evidences indicated that shear stress is critical in orchestrating gene expression in cardiovascular disease. It is necessary to identify the mechanism of shear stress influencing gene expression in physiology and pathophysiology conditions. This paper aimed to identify candidate hub genes and its transcription factors with bioinformatics. Methods We analyzed microarray expression profile of GSE16706 to identify differentially expressed genes (DEGs) in low shear stress (1 dyne/cm2) treated human umbilical vein endothelial cells (HUVECs) compared with static condition for 24 h. Results 652 DEGs, including 333 up-regulated and 319 down-regulated DEGs, were screen out. Functional enrichment analysis indicated enrichment items mainly included cytokine-cytokine receptor interaction and cell cycle. Five hub genes (CDC20, CCNA2, KIF11, KIF2C and PLK1) and one significant module (score = 17.39) were identified through protein–protein interaction (PPI) analysis. Key transcriptional factor FOXC1 displayed close interaction with all the hub genes via gene-transcriptional factor network. Single-gene GSEA analysis indicated that CDC20 was linked to the G2M_CHECKPOINT pathway and cell cycle pathway. Conclusions By using integrated bioinformatic analysis, a new transcriptional factor and hub-genes network related to HUVECs treated with low shear stress were identified. The new regulation mechanism we discovered may be a promising potential therapeutic target for cardiovascular disease.

Abstract: This study has selected a vortex tracking algorithm scheme for simulating the activity of tropical cyclone in the Vietnam East Sea by CCAM model. The results show that the CCAM model is able to simulate well the large scale in each month through a reasonable description of the movement rules of the tropical cyclone in the study area. Then, this vortex tracking algorithm scheme was applied to test the seasonal forecast with the outputs of the CCAM model with a resolution of 20km for September 2018 and October 2018. The obtaining results are forecasted quite closely in terms of both quantity and high potential occurrence areas of the tropical cyclone when compared with reality. In particular, for October 2018, although the activity area of ​​the tropical cyclone - YUTU is significantly different from the multi-year average activity position, the seasonal forecast results are obtained from the 120 members of the CCAM model captured this difference. 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