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Thematische Bibliographien / Hmotnostní spectrometrie / Dissertationen

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Dissertationen zum Thema „Hmotnostní spectrometrie“

Autor: Grafiati

Veröffentlicht am 28. Juni 2021

Zuletzt aktualisiert am 29. Juli 2025

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1

Navrátilová, Romana. "Strukturní studium karboranů s využitím hmotnostní spektrometrie." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216532.

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Boron cluster compounds are substances clearly synthetic, which don't exist outdoors. This group covers boranes, heteroboranes and their derivatives. They are synthesized and examined mostly for their extraordinary structural and bonding properties. Their study also brought many remarkable findings and even allowed practical use of these compounds in science and technology. This thesis is focused on the application of mass spectrometry for the identification of boranes and heteroboranes and on the study of their fragmentation mechanisms using tandem mass spectrometry on spherical ion trap.

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Beneš, Ondřej. "Zpracování a visualizace hmotnostních spekter." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2014. http://www.nusl.cz/ntk/nusl-235426.

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One of new techniques in the field of analytical chemistry, which has more and more practical use, is mass spectrometry imaging. With its ability to record representation of substances in samples during the tissue analyze arise problem with a lot of output data which needs to be handled programmatically. The goal of this work is to create an software for processing and visualization data of new standard imzML. As a part of the work, the field of mass spectrometry, primarily MALDI TOF mass spectrometry, is briefly introduced. There are also introduced some methods for mass spectrometry data pre

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3

Valla, Martin. "Systém pro zpracovaní skóre z metod identifikace proteinů v tandemové hmotnostní spektrometrii." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217552.

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The goal of my diploma thesis was finding a suitable method for unifying score values from various protein identification search tools in MS/MS mass spectrometry into one single score value. Data coming from the output of mass spectrometer are processed in two independent search tools Mascot and X!Tandem. These were selected especially for their wide usage in proteomic labs. Both results are evaluated through newly designed function and unified by single valued score clearly identifying found proteins. Newly designed scoring value is called Matascore and function producing this score was imple

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4

Čumová, Martina. "Využití hmotnostní spektrometrie ke stanovení markerů oxidativního stresu a mykotoxinů." Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-233402.

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The first topic presented in the dissertation thesis is determination of isoprostanes as markers of oxidative stress and other compounds affected by presence of oxidative stress. Isoprostanes iPF2-III, iPF2-VI, iPF2-VI, astaxanthin and polyunsaturated fatty acids (PUFA), especially arachidonic acid (AA) were monitored in Atlantic salmon eggs (Salmo salar). Methods for the determination of these compounds have been developed and optimized using chromatographic separation coupled to conventional or mass spectrometric detection. Freshly laid eggs, eyed embryos and non-viable eggs were used to tes

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Čumová, Martina. "Využití separačních metod s hmotnostní detekcí pro studium degradačních produktů nových polymerních materiálů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216540.

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Synthetic polymer materials have been a part of human life since the 20th century. A variety of their use had resulted in a growing demand for their mass production, which then led to their increasing accumulation at waste dumps. After their deposition, these materials are exposed to many environmental factors causing their decomposition and subsequent release of the degradation products into the environment. This thesis is focused on the analysis of the photodegradation products from synthetic polymer materials and particularly polyurethane foams modified with the biodegradable fillers. One d

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6

Žvaková, Veronika. "Detailní charakterizace pyrolýzního oleje pomocí separačních technik a hmotnostní spektrometrie." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2017. http://www.nusl.cz/ntk/nusl-316220.

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The aim of this work is detailed characterization of pyrolysis oil by advanced analytical separation methods and mass spectrometry. During the pyrolysis process decomposition of complex organic material take place in the presence of high temperatures and the absence of oxygen. Condensable part of vapours and aerosols forms viscous liquid called pyrolysis oil which is a complex mixture of large amount of compounds. In this work samples from microwave pyrolysis of woodchips and sewage sludge from wastewater treatment plant Brno-Modřice were analysed. Samples were provided by Bionic Laboratories

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7

Jurasová, Lenka. "Stanovení vybraných hormonálních přípravků pomocí kapalinové chromatografie s hmotnostním detektorem." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2012. http://www.nusl.cz/ntk/nusl-216864.

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This master’s thesis is focused on optimizing of the analytical method for the determination of selected drugs - estrone, estradiole, ethynylestradiol, estriole, diethylstilestrole, norethindrone, mestranole and progestrone in wastewater using liquid chromatography with mass spectrometry (LC/MS). Solid phase extraction (SPE) using Oasis HLB cartridges was chosen extraction method Different mobile phase, temperature, flow rates and mobil phase gradient were during the process of optimization. Samples of the wastewater were collectedat the inflow and outlow of the wastewater treatment plant in B

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8

Kuba, Pavel. "Zpracování a vizualizace dat z hmotnostního spektrometru typu TOF-MALDI." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2012. http://www.nusl.cz/ntk/nusl-230019.

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This thesis describes the development of control applications for the deposition machine and mass spectrometer. Thesis describes operation principles of both devices and their hardware specifications. Thesis also describes the design of developed applications. Functionality was tested on series of real measurements.

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9

Trčková, Marie. "Využití metody LC/MS k analýze vybraných přírodních fyziologicky aktivních látek." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216232.

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Presented work is focused on application of combined instrumental method RP-HPLC/ESI-MS in analysis of several groups of natural compounds with positive physiological activities. Especially some antioxidants were studied in commonly and abundantly consumed food. Moreover some other substances than standard compounds were observed in complex dies. In conclusion the HPLC/ESI-MS method is comparatively advantageous in phenolic analysis, while another type of ionisation would be used in case of carotenoid compound.

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Obalil, Jiří. "Miniaturizované techniky pro analýzu průmyslových kvasinek." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216334.

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Carotenoids are natural pigments that have antioxidation and antimutagenic abilities. They are produced with the help of new technological methods. For example, carotenoid yeast Rhodotorula glutinis produces -carotene with the yield of up to 6 – 10 mg/g of the dry substance. The method of the mass spectrometry with the nanoelectrospray in the positive mode was optimized for the determination of -carotene, lycopene and astaxanthin in this project. Ionizing voltage of 4 kV and the sample flow rate of 15 – 80 nl/min through the spray silica fused capillary with the internal diameter of 25 µm were

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Burešová, Jitka. "Využití separačních metod pro studium vybraných léčiv a pesticidů ve vodách." Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-234424.

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This work is focused on the study of penetration of pesticides and pharmaceuticals in the aquatic environment. The aim was to develop and verify the optimal method for long-term monitoring of the occurrence of selected pesticides and pharmaceuticals in surface waters. Two frequently used analgesics ibuprofen and diclofenac, and carbamazepine that is used to treat epilepsy, were chosen from the group of drugs. A group of 41 pesticides were selected for monitoring too. The attention was focused on a group of urea pesticides and phenoxyacetic acids, as well as on selected pesticides falling to th

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12

Kerberová, Veronika. "Umělá sladidla a jejich případný vliv na ekosystém." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2017. http://www.nusl.cz/ntk/nusl-295680.

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This diploma thesis deals with the determination and ecotoxicological assessment of artificial sweeteners. The diploma was focused on monitoring the most commonly used sweeteners, which includes acesulfam-K, cyclamate, saccharin and sucralose. These sweeteners were analyzed by liquid chromatography with mass spectrometry, using solid phase extraction for their concentration. The samples for their determination were taken from wastewater treatment plant in Modrice. Artificial sweeteners are synthetically produced substances, which is the main reason for their occurrence in waste water. Some of

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13

Guryčová, Hana. "Syntetické vonné látky ve vodním ekosystému." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216539.

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Synthetic fragrances or MUSK compounds are artificialy synthetised organic substances, used as fragrant constituents in various homecare products, such as cosmetics, detergents, air fresheners etc. The aim of diploma thesis was the quantification of some selected MUSK compounds in the influent and efluent of the water treatment plants.The method of quantification of MUSK compounds in organic matrix was also optimised during the research. Two modifications of solid phase microextraction were tested and evaluated. The analytes were identified and quantified by gas chromatography - mass spectrome

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14

Nohelová, Gabriela. "Bisfenol A ve vodním ekosystému." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2014. http://www.nusl.cz/ntk/nusl-217085.

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This diploma thesis deals with Bisphenol A, especially with its impact on the aquatic ecosystem. Information about its properties, production and current use are summarized here. Its harmful impact on the environment, especially on the aquatic ecosystem and the human body is characterized. Also the methods of its degradation within the aquatic environment have been described. A summary of the options of a determination of Bisphenol A in water samples is incorporated and the method of gas chromatography with mass spectrometry (GC/TOF MS) and comprehensive two-dimensional gas chromatography with

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Nevědělová, Klára. "Stanovení vybraných fytofarmak a jejich metabolitů v odpadních vodách." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216446.

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This diploma thesis will be focused on the identification and quantification of selected phytopharmaceuticals ant theri transformation products using separation and spectrometric methods available in the laboratories of ICTEP.

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Hajdučková, Iva. "Stanovení výskytu toxických prvků ve vínech." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2013. http://www.nusl.cz/ntk/nusl-216907.

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The aim of this study was to determine the occurrence of selected toxic elements in wine. The theoretical part described technology of wine production and selected toxic elements (cadmium, lead, copper, zinc, nickel and chromium) in the terms of their impact on wine quality and their effects on the human organism. It provides an overview of instrumental analytical methods, which can be used for the analysis of toxic metals. In the experimental part inductively coupled mass spektrometry technique was used for the analysis of wine samples. The measured values are statistically processed and comp

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Brestovská, Marta. "Stanovení tenzidů ve vodách pomocí separačních metod." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217166.

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Surfactants are synthetically made surface-active agents contained in washing and cleaning products. They decrease the surface tension and remove dirt. Surfactants can be divided into three basic classes: anionic, cationic and non-ionic. Thanks to the massive use they penetrate into waste water and can disturb the environment. This diploma thesis deals with the occurrence of surfactants in water and their determination by separation methods, especially LC / MS.

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Borůvková, Tereza. "Stanovení polárních pesticidů v odpadních a povrchových vodách." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240792.

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This work is focused on the determination of polar pesticides in surface and waste waters. In this study Mecoprop-P, MCPA, Dichlorprop-P, 2,4-D and MCPBA in differently loaded surface waters and communal waters from sewage treatment plants were analyzed. These herbicides are included in the group identified as environmental quality standards. Some of herbicides (2,4-D and MCPA) were selected because their consumption in the Czech Republic exceeds 30 tons a year. Samples were collected prom three rivers near town of Jaroměř (Elbe, Úpa and Metuje Rivers), from one river near the town of Brno (Sv

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Cáhová, Miroslava. "Stanovení těžkých polycyklických aromatických uhlovodíků v půdách a sedimentech." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216379.

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This diploma thesis will be focused on the identification and quantification of PAHs with molecular mass exceeding 278 Da by separation and spectrometric methods available at the laboratories of ICTEP.

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Fojtíková, Nikola. "Diagnostika plazmatu generovaného v atmosféře simulující podmínky na Marsu." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-445140.

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The aim of the diploma thesis was the diagnostics of plasma generated in the atmosphere simulating conditions on Mars. This diploma thesis is focused on the simulation of Mars’ atmosphere at atmospheric pressure and normal laboratory temperature. Due to the similar conditions of Mars' atmosphere with Earth, this planet has been explored in the past as well as up to now. Mars' atmosphere is composed mostly of carbon dioxide, which makes up more than 90 % of Mars' atmosphere. A glow discharge generated in a special reactor at atmospheric pressure at a flow of pure CO2 was used to simulate the at

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Matejčíková, Eva. "Stanovení stability přípravku TauroLock." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376887.

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This diploma thesis deals with the study of the anticoagulant and antimicrobial agent TauroLockTM, which is manufactured by the german company TauroPharm GmbH. Based on the background research and taking into account the equipment of analytical laboratories of the Institute of Chemistry and Technology of Environmental Protection, optimal methods of analysis were proposed and a series of experiments were carried out which focused on the determination of the active substance and degradation products of taurolidine in TauroLockTM which was stored for a certain period of time in real conditions. T

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Doležalová, Jana. "Stanovení alkylderivátů rtuti ve vodním ekosystému." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216536.

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Diploma thesis is oriented on the question of the fate and occurence of mercury and its derivatives in water ecosystems. At first, deep literature review will be worked out. At second, the optimal analytical procedure for the determination of mercury alkylderivatives in water ecosystem compartments will be proposed and verified. At third, real samples from selected water ecosystems will be taken and analyzed.

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Veverková, Radka. "Plazmochemická depozice tenkých fluorocarbonových vrstev." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2011. http://www.nusl.cz/ntk/nusl-216697.

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Deposition of thin films is one of the most widespread applications used for the changes of surface properties of various materials. This diploma thesis is focused on diagnosing of thin film generated by a PECVD technique. The capacitively coupled RF discharge at low pressure was used for the thin films deposition using tetrafluoromethane (CF4) with addition of hydrogen (H2) as a precursor. The aim of the work was the search of optimal conditions for a hydrophobic thin layer preparation on the surface of polymer NOA. The depositions were performed in continuous and pulsed mode with different d

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Jagošová, Klára. "Hormony v čistírenských kalech." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376816.

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Currently there is a spate of interest in the presence of pharmacologically active substances in the environment. These substances are excreted in active or metabolized form and with wastewater pass the wastewater treatment plant. Current treatment technologies do not always eliminate all pharmaceuticals effectively and therefore they enter the environment. One of these active groups is the group of steroid hormones. Steroid hormones belong to the group of endocrine disruptors and they are considered to be dangerous for the ecosystems. Due to the hydrophobic character of steroid hormones they

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Šilhánková, Lenka. "Vývoj metody pro semikvantitativní stanovení mikroplastů v půdách metodou TGA-MS." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376860.

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Plasty, pronikající do životního prostředí ať už neúmyslně či záměrně, mohou být fragmentovány za vzniku částic v řádu mikrometrů (tzv. mikroplastů) a znečišťovat tak půdní systémy. Doposud vyvinuté analýzy pro stanovení mikroplastů v půdách jsou především zaměřeny na kvalitativní stanovení, nicméně i jejich provedení se zatím stále potýká s různými úskalími. Navíc, téměř všechny tyto analýzy vyžadují časově náročnou předúpravu vzorku. V této studii jsme se zaměřili na vývoj nové analytické metody pro kvantitativní stanovení mikroplastů polyvinylchloridu (PVC) a polystyrenu (PS) v půdách bez p

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Maier, Jan. "Kvantitativní analýza inhibitorů tyrozinkinázy metodou kapalinové chromatografie s hmotnostní detekcí." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-446439.

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The submitted thesis is devoted to the quantitative analysis of tyrosine kinase inhibitors, specifically imatinib and nilotinib, by liquid chromatography-mass spectrometry method. The main purpose of developing this new method of analysis at the Department of Clinical Biochemistry and Diagnostics at the University Hospital in Hradec Králové was measuring and monitoring serum or plasma concentration levels of these drugs in patients with chronic myeloid leukemia, less often in patients with gastrointestinal stromal tumour. The main task during the elaboration of the thesis was to fully optimize

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Rejšek, Jan. "Vývoj a aplikace technik ambientní hmotnostní spektrometrie." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-370610.

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(EN) Ambient mass spectrometry defines the versatile group of methods providing analysis of solid sample surfaces and liquids in an open atmospheric pressure environment, where the sample is simultaneously accessible to another treatment. Ambient mass spectrometry is a sharply developing research area in the analytical chemistry. It provides fast, direct analysis of objects without any sample pretreatment with the use of the mass spectrometer. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) equipped with software control of the sample holde

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Rejšek, Jan. "Využití ambientních ionizačních technik v hmotnostní spektrometrii." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-324164.

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Keywords: ambient ionization techniques; mass spectrometry; desorption electrospray ionization; desorption atmospheric pressure photoionization; thin layer chromatography; lipids, mass spectrometry imaging Ambient ionization technique in mass spectrometry is an ionization, which carries out in open space outside the machine and which does not require any, or only a minimal sample pretreatment. DESI (desorption electrospray ionization) and DAPPI (desorption atmospheric pressure photoionization) equipped with software control of the spray emitter position for analysis of low molecular organic co

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Kosíková, Lenka. "Chirální rozeznávání pomocí hmotnostní spektrometrie." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-304410.

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Title: Chiral recognition by mass spectrometry Author: Bc. Lenka Kosíková Department: Department of Organic Chemistry Supervisor: doc. Mgr. Jana Roithová, Ph.D. This work is focused on the use of mass spectrometry as the analytical method for chiral recognition in the gas phase. We have examined formation of complexes (S and R) isomers [dimethyl-2,2'-dihydroxy-1,1'binaphthalene-3,3'-dicarboxylate] with sodium. Chiral effect for the fomation of sodium dimers is 1,34±0,06 and that for formation of trimers 2,38±0,22 in favor of the formation of homochiral complexes. Part of the work is devoted to

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Pavlásková, Kateřina. "Hmotnostní spektrometrie v proteomice: strukturní biologie a klinické aplikace." Doctoral thesis, 2011. http://www.nusl.cz/ntk/nusl-311574.

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Mass spectrometry (MS) is a rapid, specific and very sensitive analytical method with a broad spectrum of proteomic applications such as protein identification and sequencing, 3D protein structure characterization or study of protein-protein interaction. The introduction of two ionization techniques in late 1980's that are able to ionize the large biomolecules such as proteins, oligosaccharides or nucleic acids with no or low fragmentation has started the rapidly expanding field of MS-based proteomics. The presented thesis was aimed at the application of mass spectrometric approaches to answer

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Stýblová, Marie. "Analýza přírodních organických barviv a pigmentů pomocí hmotnostní spektrometrie." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-304086.

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This thesis named "Analysis of natural organic dyes and pigments by mass spectrometry" investigates the identification of selected natural organic dyes and pigments by qualitative Laser Desorption/Ionisation - Time of Flight Mass Spectrometry analysis (LDI-TOF MS). The advantage of LDI-TOF MS method is its quick and reliable identification of low molecular weight material without preparatory chemical or time-consuming modification of samples. This method can be used for the analysis of works of art or for verifying their authenticity thanks to requiring only a little amount of sample. The LDI-

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Vopelková, Alžběta. "Využití technik hmotností spektrometrie v analýze přírodních látek." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-412220.

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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Bc. Alžběta Vopelková Supervisor: doc. Ing. Lucie Cahlíková, Ph.D. Title of diploma thesis: Using of techniques of mass spectrometry in the analysis of natural compounds This diploma thesis deals with the topic " Using of techniques of mass spectrometry in the analysis of natural compounds ". The aim of the work was to create a research work and describe the profile of alkaloids in individual genera of the family Amaryllidaceae. The introduction to the theoretical part

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Kukačka, Zdeněk. "Charakterizace struktury proteinů pomocí chemického zesítění a hmotnostní spektrometrie." Doctoral thesis, 2015. http://www.nusl.cz/ntk/nusl-334650.

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Some proteins require presence of their specific ligand, cofactor or prosthetic group for their activity. Binding of this specific molecule can cause conformational changes which permit to perform their function. In some occasions the identification of conformational changes could be really challenging task. In this thesis we describe the novel approach for monitoring structural changes in proteins using chemical cross-linking and high resolution mass spectrometry and its application on model calmodulin system. It is demonstrated that analysis using isotope-labelled cross-linking agents enable

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Pravdová, Adéla. "Separace a identifikace lipopeptidů pomocí chromatografie a hmotnostní spektrometrie." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-397588.

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5 Abstract Protein lipidation occupies an important place in the post-translational modification group. It plays an important role in the processes of cell differentiation or synaptic transmission. Changes in the presence of lipidated proteins may in some cases indicate the appearance of human diseases such as Huntington's disease, schizophrenia, or cancer. For this reason, their analysis has become desirable, but also problematic due to its specific characteristics. This work is focused on the development of conditions for measurement of lipopeptides produced by cleavage of proteins containin

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Strmeň, Timotej. "Strukturní analýza přírodních látek s větvenými alifatickými řetězci pomocí hmotnostní spektrometrie." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-347932.

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The diploma thesis focuses on analysis of fatty alcohols with mass spectrometry. Theoretical part describes distribution, main properties, usage and analytical methods for analysis of fatty alcohols. Experimental work focuses on the search for a proper derivatisation procedure for fatty alcohols, which would enable their detection with soft ionisation techniques of mass spectrometry, as well as their structural analysis. The main aim of the structural analysis is to find the methyl branching in the fragmentation spectra of derivatives of the fatty alcohols.

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Vavrušová, Aneta. "Chromatografická a hmotnostně-spektrometrická analýza lipidů novorozeneckého mázku." Doctoral thesis, 2020. http://www.nusl.cz/ntk/nusl-436933.

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Lipidomics is a rapidly expanding research field that has captured extensive attention worldwide in the past few years due to the increasing awareness of the crucial roles of lipids in biological systems. The aim of lipidomics is to comprehensively analyze all lipids, to study their structure, biological function within the cell as well as interactions of lipids with other molecules. A combination of advanced analytical techniques, such as extraction, chromatography and mass spectrometry, is an effective tool for studying all aspects of lipidomics. This dissertation thesis is based on two jour

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Křížová, Věra. "Analýza produktů reakcí ftalaldehydu s vybranými aminokyselinami." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-339149.

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This thesis focuses on analysis of reaction products of phthalaldehyde with selected amino acids using the combination of high-performance liquid chromatography with mass spectrometry. Only one product with dihydroisoindole structure is formed in the case of simple amino acids (glycine, glycine ethyl ester, alanine, α-aminobutyric acid, valine, leucine and isoleucine). Reactions of phthalaldehyde with amino acids with two amino groups (lysine, asparagine, glutamine and arginine) yield different types of compounds. Main products are formed by the interaction of both of the amino groups with one

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Míková, Radka. "Analýza lipidů novorozeneckého mázku chromatografickými metodami a hmotnostní spektrometrií." Doctoral thesis, 2016. http://www.nusl.cz/ntk/nusl-352040.

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(EN) Vernix caseosa is a white creamy substance that covers the skin of a newborn. It is produced during the third trimester by the skin of the baby and remains there until the age of one or even two weeks. It is uniquely human. In utero, vernix protects the skin from maceration, during the birth it serves as a lubricant and after the delivery it protects the baby against infection and regulates the temperature. As vernix is produced in third trimester, prematurely born infants lack it and this may lead to, among other things, suffering from desiccation and therefore heat loss. It is important

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Kuchař, Ladislav. "Tandemová hmotnostní spektrometrie sfingolipidů s aplikací pro metabolické studie a diagnostiku sfingolipidos." Doctoral thesis, 2013. http://www.nusl.cz/ntk/nusl-328211.

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In recent years, mass spectrometry (MS) become the dominant technology in lipidomic analysis and widely influenced research and diagnosis of diseases of lipid metabolism, e.g. lysosomal storage disorders (LSD) characterized by impairment of the lysosomal functions. Defects in lysosomal processing of sphingolipids SFL belong to the category of sphingolipidoses. This condition has severe and even fatal clinical outcome. The primary aim of this work was to establish quantitative and qualitative methods of SFL analysis useful for research and diagnosis of LSD. At first, semisynthesis of mass label

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Vaňátková, Petra. "Využití hmotnostní spektrometrie a dalších analytických technik pro analýzu rheniových komplexů." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-352472.

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Rhenium complexes coordinated with suitable organic ligand gain increased interest in radiopharmaceutical medicine. Besides suitable radiological properties given by a metal ion itself, detailed knowledge of overall chemical properties of formed complexes, namely their exact structures, chemical stabilities and possible degradation pathways are essential pre-requisites for their clinical application. Selected rhenium complexes with pyrogallol 2,3-dihydroxynaphthalene and catechol as strongly bound ligands were prepared by a reaction of tetrabutylammonium tetrachlorooxorhenate with twofold mola

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Brůhová, Michalčíková Regina. "Nové metody hmotnostní spektrometrie pro stopovou analýzu látek v lidském dechu." Doctoral thesis, 2019. http://www.nusl.cz/ntk/nusl-409264.

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This dissertation thesis summarizes results of experiments that have been carried out during my PhD studies related to the new mass spectrometric methods for trace gas analysis of human breath. The thesis is divided into the theoretical and experimental part. The chapter at the beginning of this dissertation summarizes the current research in the area of breath analysis. It is describing the common breath metabolites, benefits and challenges of the method for therapeutic monitoring and clinical diagnosis and current applications. The next chapter of the theoretical introduction describes the t

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Rosůlek, Michal. "Studium vlivu kofaktoru na strukturu proteinu pomocí hmotnostní spektrometrie." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-331768.

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Bacterial protein WrbA from E. coli is the founding member of a new family of FMN-dependent NAD(P)H oxidoreductases, forming a functional and structural bridge between bacterial flavodoxin and certain mammalian NAD(P)H:quinone oxidoreductase. For these reasons, protein WrbA is recently intensively studied using various analytical and computing methods. Protein WrbA participates in the protection of cells against oxidative stress, but precise function of the protein WrbA in vivo is still unknown. Protein WrbA forms multimers in solutions. In μM concentrations and at low temperature (4 řC) the p

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Tužil, Jan. "Hledání potenciálního vazebného partnera glutamátkarboxypeptidasy II pomocí hmotnostní spektrometrie." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-324149.

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English Abstract The incoming paradigm of the network (or systems) biology calls for a new high throughput tool for a wide scale study of protein-protein interactions. Mass spectrometry-based proteomics have experienced a great progress in recent years and have become an indispensable technology of elementary as well as clinical research. Glutamate carboxypeptidase II (GCPII; EC 3.5.17.21) is a transmembrane protein with two known enzymatic activities. Its expression is highly upregulated in some solid tumors and also in tumor-associated neovasculature in general. Nevertheless, none of the two

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Tsybizova, Alexandra. "Přímé monitorování reakcí katalyzovaných kovy pomocí hmotnostní spektrometrie s elektrosprejovou ionizaci." Doctoral thesis, 2016. http://www.nusl.cz/ntk/nusl-351051.

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The current dissertation thesis is focused on the investigation of metal-catalyzed reactions using electrospray ionization mass spectrometry as the primary research technique. However, other gas- phase methods such as tandem mass spectrometry, infrared multiphoton dissociation spectroscopy and quantum chemical calculations have also been involved to unravel and support the findings and proposals. As organometallic chemistry is a very broad and complicated topic, this thesis is only focused on a few projects. The fist of them is dedicated to copper acetate speciation in organic solvents, the se

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Slavata, Lukáš. "Studium interakce mezi DNA a transkripčními faktory pomocí hmotnostní spektrometrie." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-332586.

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Transcription factors play crucial regulatory role within the cell and the entire multicellular organism. The important factor is its ability to interact with other regulatory proteins and DNA. Despite the fact that a large part of the interaction network is already documented, detailed information on the structure and dynamics of protein-protein and protein-DNA complexes is still scarce. In this thesis we focused on the possibility of studying conformational changes given by the transcription factor-DNA complex formation using the methods of structural mass spectrometry: hydrogen/deuterium ex

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Zdražilová, Lucie. "Analýza obsahu dolicholu v moči u pacientů s dědičnými poruchami glykosylace pomocí hmotnostní spektrometrie." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-379509.

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Dolichol is a membrane lipid, which carries monnosaccharides and glycans for N-linked protein glycosylation and glycosylphosphatidylinositol-anchor biosynthesis occuring in endoplasmic reticulum. Its structure is composed of isoprenoid units. Dolichol is present in all tissues and in most of the membrane organelles of eukaryotic cells. Recently some types of congenital disorders of glycosylation have been described as a consequence of dolichol biosynthesis and metabolism defects, which are not detectable by standard methods. The aim of this diploma thesis was to analyze dolichol content in uri

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Mazurová, Martina. "Struktura a interakce lidského regulačního proteinu 14-3-3: fotoafinitní značení in vitro a hmotnostní spektrometrie." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-345310.

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This work is focused on the interactome study of 14-3-3ζ protein, a regulatory protein found in all eucaryotic cells. An important 14-3-3 protein feature is the ability to bind a number of structurally and functionally distinct protein ligands. This link is usually implemented through phosphorylated serine and threonine motifs. The first aim of this work is the preparation of sufficient amount of recombinant 14-3-3ζ protein with incorporated photoactivatable analogue of methionine (foto-Met, L-2-amino-5,5- azihexan acid). The four different conditions of recombinant expression in auxotrophic E

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Novák, Jiří. "Podobnostní vyhledávání v databázích hmotnostních spekter." Doctoral thesis, 2013. http://www.nusl.cz/ntk/nusl-322611.

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Shotgun proteomics is a widely known technique for identification of protein and peptide sequences from an "in vitro" sample. A tandem mass spectrometer generates tens of thousands of mass spectra which must be annotated with peptide sequences. For this purpose, the similarity search in a database of theoretical spectra generated from a database of known protein sequences can be utilized. Since the sizes of databases grow rapidly in recent years, there is a demand for utilization of various database indexing techniques. We investigate the capabilities of (non)metric access methods as the datab

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Strmeň, Timotej. "Hmotnostní spektrometrie s chemickou ionizací za atmosférického tlaku při nízkých průtocích: konstrukční řešení a využití." Doctoral thesis, 2020. http://www.nusl.cz/ntk/nusl-436920.

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(EN) The first part of the thesis describes the ionization reactions in the APCI source in the presence (enclosed configuration) and absence (open configuration) of the ion source housing. Commercial APCI source was modified and used in enclosed, as well as open configuration. All other parameters were kept the same (including the source geometry). In the positive mode, the biggest differences were observed for non-polar analytes dissolved in aprotic solvents like toluene, chloroform and carbon disulfide. Protonated species were dominant in the open configuration, while radical cations were mo

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Harazim, Eva. "Lipidomická analýza novorozeneckého mázku." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-369445.

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Methods of analytical chemistry are widely used in lipidomics. Separation techniques coupled to mass spectrometry or nuclear magnetic resonance are used very often. They make it possible to identify lipids present in the matrix in very small quantities. This work summarizes the application of modern analytical methods and instrumentation for identifying and characterizing lipids in vernix caseosa. It is shown how I contributed during the Ph.D. studies to the elucidation of the structure and characterization of unknown lipid classes followed by more detailed description of those lipid classes a

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