Thematische Bibliographien / Hesci

Auswahl der wissenschaftlichen Literatur zum Thema „Hesci“

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Veröffentlicht am 25. Juli 2024

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Zeitschriftenartikel zum Thema "Hesci"

1

Yeo, C. X., J. Rathjen und D. K. Gardner. „112. FETAL CALF SERUM AFFECTS hESC METABOLISM AND GENE EXPRESSION LEADING TO DIFFERENTIATION IN CULTURE“. Reproduction, Fertility and Development 22, Nr. 9 (2010): 30. http://dx.doi.org/10.1071/srb10abs112.

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Fetal calf serum (FCS) has conventionally been used to support the growth and maintenance of human embryonic stem cells (hESCs). FCS however, is an undefined complex mixture containing factors which potentially alter the functionality of hESCs. Inclusion of FCS during embryo culture negatively impacts embryo metabolism and viability but comparative studies on hESCs have been hindered by the lack of serum and feeder independent culture systems. Using a recently available defined culture system, the effects of FCS on hESC metabolism and pluripotentcy were investigated. Mel2 hESCs were grown at 37°C in 5% CO2 for 3 days on matrigel (hESC-qualified) coated tissue culture wells in mTeSR1 medium. hESCs were then cultured in mTeSR1 (control) or in mTeSR1 supplemented with 20% FCS from different manufacturers or knockout serum replacement (KOSR), for 96 hours. Media was renewed daily. At the end of the culture period, spent media was collected and cells were trypsinised and counted and/or collected for gene expression analysis. FCS decreased cell survival and altered hESC morphology from densely packed colonies with distinct borders into non-uniform heterogeneous populations comprising of hESC-like cells and fibroblastic-like cells with high cytoplasmic to nuclear ratios. Media analysis revealed altered cell metabolism with increased glucose consumption rates per cell (P < 0.01) with FCS supplementation, compared to cells cultured in mTeSR1 alone. Gene expression analysis revealed that FCS, regardless of its manufacturer, decreased the expression of some pluripotent markers and increased differentiation markers. A decrease in pluripotent gene expression was also observed in hESCs cultured with KOSR compared to mTeSR1 alone. Maintenance of homogeneity in hESC populations is crucial for the advancement of hESC clinical therapies. This study demonstrates that FCS promotes heterogeneity and impacts the metabolic function and gene expression of hESCs thereby supporting the need for serum-free culture systems as standard practice in hESC culture.
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Wang, Lisheng, Li Li, Pablo Menendez, Chantal Cerdan und Mickie Bhatia. „Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts or conditioned media are capable of hematopoietic development“. Blood 105, Nr. 12 (15.06.2005): 4598–603. http://dx.doi.org/10.1182/blood-2004-10-4065.

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Abstract To date, hematopoietic development of human embryonic stem cells (hESCs) has been limited to cell lines cultured in the presence of either mouse embryonic fibroblasts (MEFs) or MEF-conditioned media (MEF-CM). Anonymous xenogenic factors from MEFs or MEF-CM complicate studies of hESC self-renewal and also raise concerns for the potential clinical applications of generating primitive hematopoietic cells from hESC lines maintained under these ambiguous conditions. Here, we demonstrate that hESCs can be cultured over 30 passages in defined conditions in the absence of MEFs or MEF-CM using only serum replacement (SR) media and high concentrations of basic fibroblast growth factor (SR-bFGF). Similar to hESCs cultured in MEF-CM, hESCs cultured in SR-bFGF sustained characteristics of undifferentiated hESCs, proliferative potential, normal karyotype, in vitro and in vivo 3 germ-layer specification and gave rise to hemogenic-endothelial precursors required for subsequent primitive hematopoietic development. Our report demonstrates that anonymous factors produced by feeder cells are not necessary for hESC maintenance and subsequent hematopoietic specification, thereby providing a defined system for studies of hESC self-renewal and hESC-derived hematopoiesis. (Blood. 2005;105:4598-4603)
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Martin, Colin H., Petter S. Woll, Zhenya Ni, Juan Carlos Zúñiga-Pflücker und Dan S. Kaufman. „Differences in lymphocyte developmental potential between human embryonic stem cell and umbilical cord blood–derived hematopoietic progenitor cells“. Blood 112, Nr. 7 (01.10.2008): 2730–37. http://dx.doi.org/10.1182/blood-2008-01-133801.

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Abstract Hematopoietic progenitor cells derived from human embryonic stem cells (hESCs) develop into diverse mature hematopoietic lineages, including lymphocytes. Whereas functional natural killer (NK) cells can be efficiently generated in vitro from hESC-derived CD34+ cells, studies of T- and B-cell development from hESCs have been much more limited. Here, we demonstrate that despite expressing functional Notch-1, CD34+ cells from hESCs did not derive T cells when cocultured with OP9 cells expressing Delta-like 1, or in fetal thymus organ culture. hESC-derived CD34+ cells also did not produce B cells in vitro. In contrast, CD34+ cells isolated from UCB routinely generated T and B cells when cultured in the same conditions. Notably, both undifferentiated hESCs, and sorted hESC-derived populations with hematopoietic developmental potential exhibited constitutive expression of ID family genes and of transcriptional targets of stem cell factor–induced signaling. These pathways both inhibit T-cell development and promote NK-cell development. Together, these results demonstrate fundamental differences between hESC-derived hematopoietic progenitors and analogous primary human cells. Therefore, hESCs can be more readily supported to differentiate into certain cell types than others, findings that have important implications for derivation of defined lineage-committed populations from hESCs.
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Li, Zongjin, Bryan Smith, Mei Huang, Xiaoyan Xie, Sanjiv Sam Gambhir und Joseph Wu. „Endothelial Differentiation of Human Embryonic Stem Cell and Functional Blood Vessels Formation in Vivo“. Blood 112, Nr. 11 (16.11.2008): 5455. http://dx.doi.org/10.1182/blood.v112.11.5455.5455.

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Abstract Background: Human embryonic stem (hES) cells are distinguished by their capacity for self-renewal and pluripotency. Here we characterize the differentiation of hES cell-derived endothelial cells (hESC-ECs), use molecular imaging techniques to examine their survival and function in vivo. Methods and Results: Here we introduced two-step procedures to increase endothelial differentiation efficiency of hESCs by subcultured embryoid bodies (EBs) in collagen. Single cell suspensions from EBs sprouting in collagen were obtained by treatment with collagenase and Liberase Blendzyme IV and CD31/CD144 positive cells were isolated by FACScan. After isolation, these hESC-ECs express endothelial cell markers similar to HUVEC, form vascular-like channels, and incorporate DiI-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) in vitro. Real time PCR array described increasing endothelial transcription. Using whole genome microarrays, we investigated the hESCs derived endothelial cells (hESC-ECs) transcriptome that occur among sequenced hESCs differentiation processes and human umbilical vein endothelial cells (HUVECs). We found that hESC-ECs expressed endothelial gene at pattern similar to HUVECs. By intravital microscope, we demonstrated that hESC-ECs can form function vessels with blood flow. Conclusion: Taken together, two-steps procedures increased the endothelial differentiation efficiency hESCs, and hESC-ECs can form functional vessel in vivo.
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Khan, Firdos Alam, Dana Almohazey, Munthar Alomari und Sarah Ameen Almofty. „Isolation, Culture, and Functional Characterization of Human Embryonic Stem Cells: Current Trends and Challenges“. Stem Cells International 2018 (26.08.2018): 1–8. http://dx.doi.org/10.1155/2018/1429351.

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Human embryonic stem cells (hESCs) hold great potential for the treatment of various degenerative diseases. Pluripotent hESCs have a great ability to undergo unlimited self-renewal in culture and to differentiate into all cell types in the body. The journey of hESC research is not that smooth, as it has faced several challenges which are limited to not only tumor formation and immunorejection but also social, ethical, and political aspects. The isolation of hESCs from the human embryo is considered highly objectionable as it requires the destruction of the human embryo. The issue was debated and discussed in both public and government platforms, which led to banning of hESC research in many countries around the world. The banning has negatively affected the progress of hESC research as many federal governments around the world stopped research funding. Afterward, some countries lifted the ban and allowed the funding in hESC research, but the damage has already been done on the progress of research. Under these unfavorable conditions, still some progress was made to isolate, culture, and characterize hESCs using different strategies. In this review, we have summarized various strategies used to successfully isolate, culture, and characterize hESCs. Finally, hESCs hold a great promise for clinical applications with proper strategies to minimize the teratoma formation and immunorejection and better cell transplantation strategies.
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Kim, Yoon Young, Seung-Yup Ku, Zev Rosenwaks, Hung Ching Liu, Sun Kyung Oh, Shin Yong Moon und Young Min Choi. „Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage“. Evidence-Based Complementary and Alternative Medicine 2011 (2011): 1–8. http://dx.doi.org/10.1155/2011/167376.

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Human embryonic stem cells (hESCs) have capacities to self-renew and differentiate into all cell typesin vitro. Red ginseng (RG) is known to have a wide range of pharmacological effectsin vivo; however, the reports on its effects on hESCs are few. In this paper, we tried to demonstrate the effects of RG on the proliferation and differentiation of hESCs. Undifferentiated hESCs, embryoid bodies (EBs), and hESC-derived cardiac progenitors (CPs) were treated with RG extract at 0.125, 0.25, and 0.5 mg/mL. After treatment of undifferentiated hESCs from day 2 to day 6 of culture, BrdU labeling showed that RG treatment increased the proliferation of hESCs, and the expression of Oct4 and Nanog was increased in RG-treated group. To find out the effects of RG on early differentiation stage cells, EBs were treated with RG extract for 10 days and attached for further differentiation. Immunostaining for three germ layer markers showed that RG treatment increased the expressions of Brachyury and HNF3βon EBs. Also, RG treatment increased the expression of Brachyury in early-stage and of Nkx2.5 in late-stage hESC-derived CPs. These results demonstrate facilitating effects of RG extract on the proliferation and early differentiation of hESC.
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Kumar, Deepak, Saniya Gupta, Ying Yang und Nicholas R. Forsyth. „αVβ5 and CD44 Are Oxygen-Regulated Human Embryonic Stem Cell Attachment Factors“. BioMed Research International 2013 (2013): 1–9. http://dx.doi.org/10.1155/2013/729281.

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Human embryonic stem cells (hESCs) have great potential for clinical therapeutic use. However, relatively little is known of the mechanisms which dictate their specificity of adhesion to substrates through adhesion proteins including integrins. Previous observations demonstrated enhanced clonogenicity in reduced oxygen culture systems. Here, we demonstrated via antibody blocking experiments thatαVβ5 andα6 significantly promoted hESC attachment in 2% O2only, whereas blockage of CD44 inhibited cell attachment in 21% O2alone. Immunofluorescence confirmed expression ofαVβ5 and CD44 in both 2% O2and 21% O2cultured hESCs while flow cytometry revealed significantly higherαVβ5 expression in 2% O2versus 21% O2cultured hESCs and higher CD44 expression in 21% O2versus 2% O2cultured hESCs. Adhered hESCs following blockage ofαVβ5 in 2% O2displayed a reduction in nuclear colocalisation of Oct-4 and Nanog with little effect observed in 21% O2. Blockage of CD44 had the converse effect with dramatic reductions in nuclear colocalisation of Oct-4 and Nanog in 21% O2cultured hESC which retained adherence, but not in 2% O2cultured cells. Identification of oxygen-dependent substrate attachment mechanisms in hESCs has the potential to play a role in the development of novel substrates to improve hESC attachment and culture.
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Svensson, Bengt, Srinivasa R. Nagubothu, Christoffer Nord, Jessica Cedervall, Isabell Hultman, Lars Ährlund-Richter, Anna Tolf und Stellan Hertegård. „Stem Cell Therapy in Injured Vocal Folds: A Three-Month Xenograft Analysis of Human Embryonic Stem Cells“. BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/754876.

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We have previously shown that human embryonic stem cell (hESC) therapy to injured rabbit vocal folds (VFs) induces human tissue generation with regained VF vibratory capacity. The aims of this study were to test the sustainability of such effect and to what extent derivatives of the transplanted hESCs are propagated in the VFs. The VFs of 14 New Zealand rabbits were injured by a localized resection. HESCs were transplanted to 22 VFs which were analyzed for persistence of hESCs after six weeks and after three months. At three months, the VFs were also analyzed for viscoelasticity, measured as dynamic viscosity and elastic modulus, for the lamina propria (Lp) thickness and relative content of collagen type I. Three months after hESC cell therapy, the dynamic viscosity and elastic modulus of the hESC treated VFs were similar to normal controls and lower than untreated VFs (p≤0.011). A normalized VF architecture, reduction in collagen type I, and Lp thickness were found compared with untreated VFs (p≤0.031). At three months, no derivatives of hESCs were detected. HESCs transplanted to injured rabbit VFs restored the vibratory characteristics of the VFs, with maintained restored function for three months without remaining hESCs or derivatives.
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Son, Mi-Young, Janghwan Kim, Hyo-Won Han, Sun-Mi Woo, Yee Sook Cho, Yong-Kook Kang und Yong-Mahn Han. „Expression profiles of protein tyrosine kinase genes in human embryonic stem cells“. REPRODUCTION 136, Nr. 4 (Oktober 2008): 423–32. http://dx.doi.org/10.1530/rep-08-0080.

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Complex signaling pathways operate in human embryonic stem cells (hESCs) and are coordinated to maintain self-renewal and stem cell characteristics in them. Protein tyrosine kinases (PTKs) participate in diverse signaling pathways in various types of cells. Because of their functions as key molecules in various cellular processes, PTKs are anticipated to have important roles also in hESCs. In this study, we investigated the roles of PTKs in undifferentiated and differentiated hESCs. To establish comprehensive PTK expression profiles in hESCs, we performed reverse transcriptase PCR using degenerate primers according to the conserved catalytic PTK motifs in both undifferentiated and differentiated hESCs. Here, we identified 42 different kinases in two hESC lines, including 5 non-receptor tyrosine kinases (RTKs), 24 RTKs, and 13 dual and other kinases, and compared the protein kinase expression profiles of hESCs and retinoic acid-treated hESCs. Significantly, up- and downregulated kinases in undifferentiated hESCs were confirmed by real-time PCR and western blotting. MAP3K3, ERBB2, FGFR4, and EPHB2 were predominantly upregulated, while CSF1R, TYRO3, SRC, and GSK3A were consistently downregulated in two hESC lines. Western blot analysis showed that the transcriptional levels of these kinases were consistent with the translational levels. The obstruction of upregulated kinases’ activities using specific inhibitors disturbed the undifferentiated status and induced the differentiation of hESCs. Our results support the dynamic expression of PTKs during hESC maintenance and suggest that specific PTKs that are consistently up- and downregulated play important roles in the maintenance of stemness and the direction of differentiation of hESCs.
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Denham, Mark, Brock J. Conley, Fredrik Olsson, Lerna Gulluyan, Timothy J. Cole und Richard Mollard. „A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species“. American Journal of Physiology-Lung Cellular and Molecular Physiology 292, Nr. 5 (Mai 2007): L1241—L1247. http://dx.doi.org/10.1152/ajplung.00440.2006.

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Human embryonic stemlike cells (hESCs) are pluripotent cells derived from blastocysts. Differentiating hESCs into respiratory lineages may benefit respiratory therapeutic programs. We previously demonstrated that 24% of all mouse embryonic stem cell (mESC) derivatives cocultured with embryonic day 11.5 (E11.5) mouse lung rudiments display immunoreactivity to the pneumonocyte II specific marker surfactant-associated protein C (Sftpc). Here we further investigate the effects of this inductive niche in terms of its competence to induce hESC derivative SFTPC immunoreactivity and the expression of other markers of terminal lung secretory units. When hESCs were cocultured as single cells, clumps of ∼10 cells or embryoid bodies (EBs), hESC derivatives formed pan-keratin-positive epithelial tubules at high frequency (>30% of all hESC derivatives). However, human-specific SFTPC immunoreactivity associated with tubule formation only at low frequency (<0.1% of all hESC derivatives). Human-specific SFTPD and secretoglobin family 1A member 1 ( SCGB1A1, also known as CC10) transcripts were detected by PCR after prolonged culture. Expression of other terminal lung secretory unit markers ( TITF1, SFTPA, and SFTPB) was not detected at any time point analyzed. On the other hand, hESC derivatives cultured as plated EBs in media previously demonstrated to induce Sftpc expression in isolated mouse fetal tracheal epithelium expressed all terminal lung secretory unit markers examined. mESCs and hESCs thus display fundamental differences in their response to the E11.5 mouse lung inductive niche, and these data provide an important step in the delineation of signaling mechanisms capable of efficiently inducing hESC differentiation into terminal secretory units of the lung.
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Dissertationen zum Thema "Hesci"

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Müller-Kelwing, Karin. „Michael Hesch“. Böhlau Verlag, 2020. https://slub.qucosa.de/id/qucosa%3A75086.

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Hesch, Christian [Verfasser]. „Dynamics of continua with interfaces / Christian Hesch“. Siegen : Universitätsbibliothek der Universität Siegen, 2013. http://d-nb.info/1036776441/34.

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Chong, Tsz-yat Ian, und 莊子逸. „Inducing the progressive differentiation of hESCs into pancreatic progenitor cells“. Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/196433.

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Diabetes is a chronic disorder of the pancreas, where a decline in the insulin-producing β-cell population disrupts metabolic homeostasis. Pancreatic transplantation has shown to be effective in circumventing the problem of β-cell insufficiency. However, availability of donor islets remains an obstacle. Although progressive differentiation of embryonic stem cells (ESCs) to pancreatic β-cells is a solution, current protocols are wrought with inefficiencies. It is obvious that to realize ESC differentiation for therapy many steps need to be optimized, and this study describes improvement of Pdx1+pancreatic progenitor derivation, a critical determinant of pancreatic fate. The compounds melatonin and sPDZD2 have been suggested to act through the Protein Kinase A (PKA) pathway to exert transcriptional effects, and in particular sPDZD2 stimulates the expression of pancreatic genes in INS-1E rat pancreatic cells. This led to the hypothesis that the PKA-targeting characteristics of said molecules could be exploited for pancreatic specification through post-translational activation ofPdx1. hESCs were first induced to form definitive endoderm before treatment with melatonin and sPDZD2. Pdx1 expression induced by these molecules was then compared with levels triggered by known pancreatic progenitor inducer Indolactam V (ILV). A secondary objective of this study was to assess the endoderm induction potential of small molecules in hESCs, which claim to be potentially useful in differentiation. In this research, I show that small molecules are noticeably more challenging to use in the hESC context. Between the TGF-β pathwayactivatorsIDE-1 and 2, the latter is more potent at inducing endoderm formation, though it does not surpass the capabilities of Stauprimide, a molecule originally thought to only serve a priming purpose in mESCs.IDE-2 and Stauprimide consistently perform better than Activin A, the near universal factor for endoderm induction. Possible synergy between IDE-2 and Stauprimide was explored, but their combination appears detrimental to Sox17expression. Subsequent pancreatic differentiation was also inefficient, and my results affirm the immaturity of chemically-induced endoderm by contrasting with mainstream means of endoderm induction; levels of endoderm marker expression between the two methods are millions of folds apart. This work exposes the risks of using small molecules, and they necessitate proper characterization before being adopted for differentiation. Most favorably, both sPDZD2 and melatonin were able to trigger Pdx1 expression in STEMDiffTm derived definitive endoderm; 10 and 30folds respectively, comparable to the known Pdx1 inducer ILV (25 folds). I also reveal concentration-mediated differentiation and proliferative purposes of ILV and sPDZD2, which are highly reminiscent of the signaling mechanisms involved during pancreatic development. Preliminary quantification of Pdx1+ cells suggest that high concentrations of ILV and sPDZD2 favor self-renewal of Pdx1+ progenitors, whilst lower doses elevate Pdx1 expression. Demonstration of Pdx1 at both gene and protein expression levels was encouraging, but it remains uncertain if melatonin and sPDZD2 manipulate PKA signaling to exert Pdx1 promoting effects. My work supports the use of melatonin as a candidate for pancreatic differentiation, and suggests involvement of sPDZD2 in deriving and expanding progenitors during pancreatic organogenesis.
published_or_final_version
Biochemistry
Master
Master of Philosophy
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Hesch, Christian [Verfasser]. „Mechanische Integratoren für Kontaktvorgänge deformierbarer Körper unter großen Verzerrungen / Christian Hesch“. Siegen : Universitätsbibliothek Siegen, 2008. http://d-nb.info/999228978/34.

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Kukuczková, Anna. „Vnitřní procesy herce“. Master's thesis, Akademie múzických umění v Praze.Hudební a taneční fakulta. Knihovna, 2017. http://www.nusl.cz/ntk/nusl-369707.

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The thesis examines the processes as they occur in the actor ́s mind during work on the character while rehearsing and acting. It also describes extensively the steps prior to the rehearsal process and acting from the actor ́s point of view regarding the actor ́s mental states. Additionally, the focus is on various approaches to character building during rehearsals and on various wals of working with the text. In her analyses, the authoress is aware of the specific differences of the theatrical genres. The thesis is based largely on her experience.
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Murray, Karen T., Carolyn S. Merriman und Carolyn Adamson. „Use of the HESI Admission Assessment to Predict Student Success“. Digital Commons @ East Tennessee State University, 2008. https://dc.etsu.edu/etsu-works/8518.

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This study examined the value of the HESI Admission Assessment in predicting student success. Associate degree (N ≤ 68) and baccalaureate (N ≤ 69) nursing students took the HESI Admission Assessment after acceptance into the nursing programs for the purpose of identifying their academic weaknesses and focusing their remediation efforts. Findings indicated that the HESI Admission Assessment was a valid predictor of students' academic ability to succeed in the nursing programs. In the associate degree nursing program, HESI Admission Assessment scores were significantly positively correlated with 88.89% of all nursing course grades in the program and 100% of the beginning-level course grades. In the baccalaureate nursing program, HESI Admission Assessment scores were significantly positively correlated with 50.00% of all nursing course grades in the program and 80.00% of beginning-level course grades. Furthermore, associate degree nursing students who completed the program had significantly higher HESI Admission Assessment scores than those who did not complete the program.
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Hamidi, Sofiane. „Etudes de la monocytopoïèse issue de cellules hESC ou iPSC“. Paris 7, 2013. http://www.theses.fr/2013PA077266.

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Mon travail a consisté à caractériser la monocytopoïèse dérivée d'hESC et à confirmer in-vitro que les macrophages obtenus partageaient les mêmes fonctions homéostatiques que ceux dérivés de foetus. J'ai ensuite dérivé des lignées d'iPSC à partir de cellules CD34+ d'un sujet sain et muté JAK2V617F. Ma première étude a consisté à comparer les macrophages issus de la différenciation d'hESC à ceux issus d'iPSC contrôle. Cette partie de mon travail a permis de montrer que les macrophages issues d'IPSC sont très proches de ceux dérivées de hESC mais présentent des différences significatives dans la sécrétion de certaines molécules de la réponse inflammatoire à l'IFN-y et au LPS. Enfm nous avons souhaité étudier grâce à la technologie des iPSC l'implication de la voie JAK/STAT dans le phénotype fonctionnel des macrophages embryonnaires et dans leur défaut de réponse à l'IFN-7 et au LPS. Les résultats préliminaires ne montrent ni de différences dans la polarisation des monocytes/ macrophages JAK2V617F, ni d'altération dans l'activation de STAT1 via l'IFN-7. Les différences ontogéniques sont donc liées soit à des modifications épigénétiques dans les gènes impliqués dans l'inflammation ou dans les partenaires de STAT1. Un travail encore plus préliminaire a été d'explorer les éventuelles indépendances au bFGF des iPS( pluripotentes. En effet un travail de Griffiths et al a montré que les mESC JAK2V617F pouvaient mainteni leur phénotype pluripotent en l'absence de LIF via son action nucléaire indirecte sur le promoteur de Nanog Les résultats préliminaires montrent que les lignées d'IPSC JAK2V617F humaines pouvaient maintenir li phénotype pluripotent et cela en l'absence de bFGF
My work was focused on the monocyte and macrophage lineages. We have shown that Monocyte/macrophage derived from ES cells are cells extremely specialized in tissue remodeling, pro-angiogenesis and immune suppression but with low inflammatory potential. I have investigated the characteristics of monocytes/macrophages from iPS. Those monocyte/macrophage were quite similar to hESC derived, but exhibited more inflammatory potential suggesting some incomplete reprogrammation during derivation of IPS. We hypothesize that the decrease or absence of inflammatory potential of the monocyte/macrophages could be related to a decrease activation of the JAK2/STAT pathway induced by IFN-y and GM-CSF, two cytokines implicated in MI polarization. In this purpose we derive iPS from patient harboring the JAK2V617F mutation, a gain of fonction mutation associated with myéloproliférative neoplasm. In preliminary results, no significant differences were observed in the polarization of JAK2V617F or JAK2WT monocyte/Macrophages derived from IPS. Furthermore we found that IFN-y was capable to normally induce STAT1 activation in these cells suggesting that the blockage in inflammatory response is downstream STAT1 and may be related to epigenetic regulation of inflammatory gens. Finally I have obtained preliminary results showing that JAK2V617F may induce independence to bFGF of the pluripotent iPSC, a result very similar to those reported by Griffiths and al on JAK2V617F mESC who could maintain their pluripotent phenotype without addition of LIF. This was not related to the induction of the canonical STAT pathway but to an effect of nuclear JAK2 on the epigenetic regulation of Nanog
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Roleček, Vít. „Herec a strach“. Master's thesis, Akademie múzických umění v Praze.Divadelní fakulta. Knihovna, 2016. http://www.nusl.cz/ntk/nusl-263138.

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In my master thesis, I am trying to name the origins and signals of an actor`s fear. I have came from my own existing stage experiences previously and whilst studying classical acting at the Dramatic Faculty of the Academy of Performing Arts in Prague, and also from my first experiences with differing professional groups from theatres from around the Czech Republic. My thesis is supported by professional literature, with which one I have read during my studies at the Academy, and it is inspired by talks and by consultation with people who are active in an artistic field. In my work I reflect my mental prosesses, states and blocks, not only during stage creations, but also in my personal life. I think that a fear can rule an actor and cause stage fright. This is why I have decided to choose this subject for my thesis.
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Křivánková, Jindřiška. „Současný herec v mimickém divadle“. Master's thesis, Akademie múzických umění v Praze.Hudební a taneční fakulta. Knihovna, 2013. http://www.nusl.cz/ntk/nusl-177936.

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This magister thesis is about the task of contemporary performer in mime theater. The thesis deals with a question of authorship and physical being of the performer on the stage. The thesis is based on analysis of graduation performence of Jindřiška Křivánková titled The hunters and on the basis of this analysis entitle principals of authorial theater and contemporary acting.
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Sitková, Kristina. „Herec a monolog“. Master's thesis, Akademie múzických umění v Praze.Divadelní fakulta. Knihovna, 2014. http://www.nusl.cz/ntk/nusl-202472.

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This thesis deals with theme The actor and the monologue. After defining the concept of monologue, there is an excursion into history and the development of the monologue. There are given personal experiences of working on monologues during the studies and talks about transformation of the ideas about monologue. In the end there is also definition of the concept of monodrama and experiences with unfinished work on monodrama by Ivan Vyrypajev called July.
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Bücher zum Thema "Hesci"

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Mauerová, Nad̕a. Herci. V Praze: Středočeské nakl. a knihkupectví, 1987.

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2

Mauerová, Naďa. Herci. V Praze: Středočeské nakl. a knihkupectví, 1987.

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3

Holanec, Václav. Herci. Praha: Knižní klub, 2012.

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4

R, Ramaty, und Mandzhavidze Natalie, Hrsg. High energy solar physics: Anticipating HESSI : proceedings of a conference held in College Park, Maryland, 18-20 October, 1999. San Francisco, Calif: Astronomical Society of the Pacific, 2000.

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5

Pravda, Jiří. Přijeli herci, zavřete slepice. Helvetia: Cramerius, 1987.

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6

Fikejz, Miloš. Český film: Herci a herečky. Praha: Libri, 2006.

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Čavojský, Ladislav. Prví a prvoradí herci SND. Bratislava: TÁLIA-press, 1993.

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Fikejz, Miloš. Český film: Herci a herečky. Praha: Libri, 2006.

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9

Krejča, Otomar. Otomar Krejča - divadlo jsou herci. Praha: Akademie múzických umění v Praze, 2011.

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10

Fikejz, Miloš. Český film: Herci a herečky. 2. Aufl. Praha: Libri, 2007.

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Buchteile zum Thema "Hesci"

1

Jain, Pankaj. „HESCO“. In Science and Socio-Religious Revolution in India, 11–19. New York : Routledge, 2017. | Series: Routledge studies in Asian religion and philosophy ; 20: Routledge, 2016. http://dx.doi.org/10.4324/9781315776316-3.

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Jain, Pankaj. „HESCO“. In Science and Socio-Religious Revolution in India, 20–66. New York : Routledge, 2017. | Series: Routledge studies in Asian religion and philosophy ; 20: Routledge, 2016. http://dx.doi.org/10.4324/9781315776316-4.

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3

Behbahan, Iman Saramipoor, und Mark A. Zern. „hESC-Derived Hepatocytes“. In Advances in Stem Cell Research, 49–66. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-940-2_4.

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4

Oleaga, Carlota, Gregg Legters, L. Richard Bridges, Lee Kumanchik, Candace Martin, Yunqing Cai, Mark Schnepper, Christopher W. McAleer, Christopher J. Long und James J. Hickman. „Contractile Force Readout of hESC-Cardiomyocytes“. In Methods in Pharmacology and Toxicology, 229–46. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6661-5_12.

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Liu, Ying, Uma Lakshmipathy, Ali Ozgenc, Bhaskar Thyagarajan, Pauline Lieu, Andrew Fontes, Haipeng Xue, Kelly Scheyhing, Chad MacArthur und Jonathan D. Chesnut. „hESC Engineering by Integrase-Mediated Chromosomal Targeting“. In Methods in Molecular Biology, 229–68. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-369-5_13.

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Ferrari, Deborah, Guochun Gong, Robert A. Kosher und Caroline N. Dealy. „Chondrogenic Differentiation of hESC in Micromass Culture“. In Springer Protocols Handbooks, 359–67. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-267-0_26.

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Zaninovic, Nikica, Qiansheng Zhan und Zev Rosenwaks. „Derivation of Human Embryonic Stem Cells (hESC)“. In Methods in Molecular Biology, 121–44. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0659-8_6.

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Gottweis, Herbert, Brian Salter und Catherine Waldby. „Bioethics and the Global Moral Economy of HESC Science“. In The Global Politics of Human Embryonic Stem Cell Science, 127–47. London: Palgrave Macmillan UK, 2009. http://dx.doi.org/10.1057/9780230594364_7.

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9

Hu, Bao-Yang, und Su-Chun Zhang. „Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs“. In Cellular Programming and Reprogramming, 123–37. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-691-7_8.

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Galán, Amparo, und Carlos Simón. „Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR“. In Methods in Molecular Biology, 135–50. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-369-5_8.

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Konferenzberichte zum Thema "Hesci"

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Chen, Weiqiang, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Paul H. Krebsbach und Jianping Fu. „Nanotopography Directs Fate of Human Embryonic Stem Cells“. In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80222.

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Human embryonic stem cells (hESCs) have remarkable potentials for breakthroughs in future cell-based therapeutics owing to their self-renewal capability and pluripotency [1–2]. However, their intrinsic mechanosensitivity to biophysical signals from the local cellular microenvironment is not well characterized [3–4]. In this work, we introduced a simple, yet precise, microfabrication strategy for accurate control and patterning of local nanoroughness on glass surfaces using photolithography and reactive ion etching (RIE). Our results demonstrated that nanoscale topological features could provide a potent regulatory signal over a diverse array of hESC behaviors, including their morphology, adhesion, proliferation and clonal expansion, and differentiation.
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Weiland, Johannes, und Martin Burkert. „Hessi James“. In ACM SIGGRAPH 2001 video review. New York, New York, USA: ACM Press, 2001. http://dx.doi.org/10.1145/945191.945213.

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3

Korin, Natanel, Avishay Bransky, Uri Dinnar und Shulamit Levenberg. „Modeling and Studying Human Embryonic Stem Cell Culture Conditions in Pulsed Flow Micro-Reactors“. In ASME 2008 9th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2008. http://dx.doi.org/10.1115/esda2008-59168.

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Embryonic stem (ES) cells research is a promising field for tissue engineering due to their proliferative capacity and differentiation abilities. The culture of Human Embryonic Stem Cells (hESC) in microchannel bioreactors can be valuable for hESC cell biology studies and hESC tissue engineering applications. We have previously demonstrated the long-term culture of mammalian (HFF-Human Foreskin Fibroblasts) cells in a microchannel (130μm) bioreactor under constant perfusion in a simple approach. However, hESC were found to be highly sensitive to flow and did not grow under flow rates which were proper for HFF long-term culture. Here, we propose the use of a novel automated periodic perfusion system to co-culture hESC with HFF in a microchannel bioreactor. The method is based on short temporal pulsed flows of medium renewal followed by long static incubation periods. The short pulsed exposure to shear enables shear sensitive cells (e.g., hESC) to withstand the medium flow. The present work studies experimentally and via numerical simulations the conditions required for hESC culture in a microchannel bioreactor using the periodic perfusion method. Conventional soft-lithography techniques were used to fabricate PDMS microchannels (100 μm) sealed reversibly with glass cover slides. HESC were seeded in the microchannel with HFF, incubated for several hours and then connected to a perfusion system which contained: a syringe pump, a permeable tube oxygenator, and waste container. The ability of the periodic perfusion protocols to prevent hESC de-attachment and maintain their culture was examined. Mass transport and fluid mechanics models were used to evaluate the culture conditions within the micro-bioreactor (shear stress, oxygen level, nutritious etc.). 3D finite element mass transport analysis (Comsol 3.3) was preformed to examine the oxygen levels in the microchannel as a function of time and design parameters. Altogether, the experimental results and the theoretical model indicate that the use of a periodic perfusion bioreactor is a suitable and promising method to culture hESC in a microreactor. Culturing undifferentiated human ES cell colonies in a micro-bioreactor is an initial step toward utilizing microfluidic techniques to investigate embryonic stem cell biology.
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Sun, Yubing, Luis G. Villa-Diaz, Raymond H. W. Lam, Weiqiang Chen, Paul H. Krebsbach und Jianping Fu. „Investigation of Mechanoresponsive Behaviors of Human Embryonic Stem Cells Using Microfabricated Elastomeric Post Arrays“. In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80269.

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Much effort has been recently directed to investigate how soluble factors in the local cellular microenvironment of embryonic stem cells (ESCs) regulate their fate decisions [1]; however, effects of mechanical signals in the local cellular microenvironment on the fates of ESCs are still not yet well understood. Early experimental evidence established in recent years has shown that mechanical signals and external forces experienced through interactions with extracellular matrix (ECM) mechanics can play critical roles in regulating survival, proliferation and differentiation of ESCs. However, there is still limited knowledge of how mechanical signals in the local cellular microenvironment regulate the fate decisions of human ESCs (hESCs), and advancing such knowledge will be critical for both fundamental understanding and clinical applications of hESCs. This work was thus set to explicitly investigate the mechanosensitive properties of hESCs.
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Dennis, Brian R., Robert P. Lin, Richard C. Canfield, Carol J. Crannell, A. Gordon Emslie, Gordon D. Holman, Hugh H. Hudson et al. „High-Energy Solar Spectroscopic Imager (HESSI)“. In SPIE's 1996 International Symposium on Optical Science, Engineering, and Instrumentation, herausgegeben von David M. Rust. SPIE, 1996. http://dx.doi.org/10.1117/12.259716.

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6

Creamer, Glenn. „The HESSI magnetic attitude control system“. In Guidance, Navigation, and Control Conference and Exhibit. Reston, Virigina: American Institute of Aeronautics and Astronautics, 1999. http://dx.doi.org/10.2514/6.1999-3969.

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7

D Senn, William, Gayle Prybutok, Kittipong Boonme und Victor R. Prybutok. „Development and Testing of an Education Service Quality Model [Abstract]“. In InSITE 2021: Informing Science + IT Education Conferences. Informing Science Institute, 2021. http://dx.doi.org/10.28945/4781.

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Aim/Purpose: This study builds upon theory to develop and test a parsimonious model of service quality importance, the Higher Education Service Quality Importance (HESQI) Model, for use in standardized, frequent surveys of student satisfaction in higher education service delivery. Background: Educational institutions operating in the higher education marketplace are facing increased competition for students. In order to be competitive in terms of the student services provided, these institutions need a method to evaluate and measure, on a frequent and recurring basis, the quality and performance of their services. Methodology: A survey was developed and administered to a stratified random sample of 1,400 students at a large public university in the southwestern United States. The 56% response rate was comprised of 790 total respondents. Partial least squares structural equation modelling (PLS-SEM) was used to analyze model constructs and latent variables. Reliability, validity, non-response bias, and common method bias were assessed as part of the research. Contribution: The HESQI is a compact, parsimonious instrument that can be administered in a cost-effective manner for faster, point-in-time checks of student satisfaction with less survey fatigue than larger instruments. Findings Service quality is significantly correlated with student satisfaction. The developed model is capable of explaining nearly 70% of the variance in student perceptions of satisfaction. Recommendations for Practitioners: The developed HESQI instrument addresses the need for a quick and easy measurement instrument to assess student satisfaction in higher education institutions. The HESQI instrument simplifies data collection and analysis and can be used on a frequent and ongoing basis to gain rapid insight into service and quality issues affecting students. Recommendations for Researchers: The development of the HESQI provides an instrument that researchers can use to study the delivery of auxiliary services in higher education. In addition, the methodology used has implications for how to develop and test other parsimonious instrument for use in other contexts. Impact on Society: Higher education is of critical value to societal mobility. As such providing a better experience for those who seek education is important and services are an important part of that experience. The HESQI has an important role in helping to improve that experience because it allows measuring the satisfaction with changes that are made to improve auxiliary services which are important to the overall environment and experience. Future Research: Future research may be carried out to further validate and confirm the research findings and use it in other environments. Also, research may consider a single item instrument in similar environments.
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Lin, R. P. „The High Energy Solar Spectroscopic Imager (HESSI) mission“. In Acceleration and transport of energetic particles observed in the heliosphere (ACE-2000 symposium). AIP, 2000. http://dx.doi.org/10.1063/1.1324313.

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Hasib, Musaddaqul, Zane Lybrand, Vanesa Nieto Estevez, Jenny Hsieh und Yufei Huang. „Charactering hESCs Organoids from Electrical Signals with Machine Learning“. In 2019 IEEE EMBS International Conference on Biomedical & Health Informatics (BHI). IEEE, 2019. http://dx.doi.org/10.1109/bhi.2019.8834587.

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10

Lee, Jonathan, Semih Bayraktar, Vincent Knight-Schrijver, Maria Colzani und Sanjay Sinha. „BS30 A scaffold for heart repair: using human embryonic stem cell derived (HESC) epicardial extracellular matrix to enhance hesc-cardiomyocyte function“. In British Cardiovascular Society Annual Conference, ‘Future-proofing Cardiology for the next 10 years’, 5–7 June 2023. BMJ Publishing Group Ltd and British Cardiovascular Society, 2023. http://dx.doi.org/10.1136/heartjnl-2023-bcs.243.

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Berichte der Organisationen zum Thema "Hesci"

1

Al-Chaar, Ghassan, Marion Banko, Brian Eick und Thomas Carlson. Performance of HESCO Bastion units under combined normal and cyclic lateral loading. Construction Engineering Research Laboratory (U.S.), Februar 2017. http://dx.doi.org/10.21079/11681/21447.

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Canto, Patricia, Hrsg. Mugaz haraindiko lankidetza sustatzeko ekimenetarako ikaskuntzak: Akitania Berria-Euskadi-Nafarroa euroeskualdeko esperientzia bat. Universidad de Deusto, 2022. http://dx.doi.org/10.18543/zxzo3953.

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Koaderno honen helburua da berrikuntzarako mugaz haraindiko lankidetza ekimenak bultzatzen dituzten erakundeentzat eta ekimen horietan parte hartzeko interesa dutenentzat ikaskuntzak ateratzea. Akitania Berria-Euskadi-Nafarroa mugaz haraindiko eremuan mugaz haraindiko hiru klusterren elkartean epe ertaineko ibilbidea duten entitateen portaera nola aztertu azaldu nahi dugu, mugaz haraindiko lankidetzari eta berrikuntzari dagokienez. Azterketa Klusteuro (Akitania Berriko, Euskadiko eta Nafarroako euroeskualdeko klusterren sarea) osatzen duten entitateei egindako inkesta batean oinarritzen da. Alde batetik, entitate horiek mugaz haraindiko lankidetza eragotzi edo erraztu dezaketen hesiei (berrikuntzarako mugaz haraindiko lankidetzarako hesiak) zenbateko garrantzia ematen dieten aztertu da, Klusteuron parte hartzeak hesi horiek gainditzen laguntzen duen ikusteko. Beste alde batetik, entitateek garatu ohi duten berrikuntza mota identifikatzen da, berrikuntza mota bat edo beste garatzerakoan klusterrak duen eginkizuna ulertzeko. Azkenik, mugaz haraindiko kluster batean edo berrikuntzarako mugaz haraindiko lankidetza sustatzeko beste ekimenen batean parte hartzeak ekar dezakeen balioaren inguruko hainbat ikaskuntza atera ditugu.
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