Dissertationen: „Glycoproteins“

1

Jefferies, W. A. "Lymphocyte surface glycoproteins." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355757.

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2

Clark, R. A. C. "Characterisation of neural glycoproteins." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363826.

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3

Premdjee, B. "Semi-synthesis of glycoproteins." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1434897/.

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Glycosylation is a prevalent form of post translational modification, believed to occur on over 50% of human proteins. Homogeneous forms of glycoproteins are essential for developing an understanding of how activity is mediated at a structural level. As biological origins of glycoproteins give rise to complex mixtures of glycoforms, homogeneous glycoprotein production has become an important goal. As chemical protein synthesis is often limited to sequences of 30-50 residues, access to large native glycoproteins is currently restricted to fragment based approaches. Protein semi-synthesis enables the preparation of larger proteins which can be difficult to obtain through chemical synthesis alone. Consequently, a general semi-synthetic strategy towards N-glycoproteins has been proposed and demonstrated on Interferon-β-1 (IFNβ), a 166 residue glycoprotein. A three fragment strategy was designed, relying on the chemical synthesis of a short glycopeptide segment and recombinant expression of the two flanking domains. Homogeneity was established through the chemical synthesis of a glycopeptide containing a natively linked N-acetylglucosamine (GlcNAc), also enabling the selective transfer of complex oligosaccharides. After cloning and expression, the recombinant fragments were functionalised to allow assembly of the protein using Native Chemical Ligation. These desired protein modifications were achieved through the application of highly chemoselective reactions. These reactions were also applied towards the generation of N-glycopeptides compatible with the ligation strategy. Further to this, existing methods enabling the direct synthesis of functionalised N-glycopeptides were also explored. After glycopeptide synthesis, endoglycosidase A enabled the transfer of oligosaccharides to the N-acetylglucosamine motif. This has allowed the preparation of the desired IFNβ glycopeptide as well as a glycosylated variant of glucagon like peptide-1. To expand the utility of endoglycosidase methodology, a novel sugar nucleotide was synthesised to facilitate the incorporation of a sialyl galactose mimic onto N-glycans. The resulting oligosaccharides may serve as novel substrates for endoglycosidases in the preparation of N-glycoprotein mimics.

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4

Crispin, Matthew D. M. "Manipulation and crystallisation of glycoproteins." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.426374.

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5

Priyanka, Pragya. "Chemoenzymatic synthesis of phosphorylated glycoproteins." Thesis, University of Canterbury. Chemistry, 2015. http://hdl.handle.net/10092/10578.

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Phosphorylation of the glycan portion of glycoproteins is an important posttranslational modification. In particular, the presence of mannose-6-phosphate (M6P) residues on high mannose glycans of lysosomal enzymes is important for the transfer of these enzymes to the lysosomes. The synthesis of different types of N-glycan structures containing M6P residues have been reported by various groups. This thesis work concerns the chemoenzymatic synthesis of phosphorylated glycoproteins, wherein M6P containing N-glycans were synthesised chemically and then enzymatically coupled to give homogeneous glycoproteins. Endo-β-N-acetylglucosaminidases (ENGases), have been employed by various research groups for the synthesis of variety of homogeneous glycopeptides and proteins by using oxazolines as activated donors. This thesis reports the synthesis of bis-phosphorylated tetrasaccharide and hexasaccharide oxazoline donors, and the use of ENGases to catalyse their transfer to peptides and proteins.

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6

Duffy, Iain. "Analysis of measles virus glycoproteins." Thesis, Queen's University Belfast, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324842.

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7

Kaye, Jane Frances. "Studies of human cytomegalovirus glycoproteins." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259731.

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8

BECKMANN, M. PATRICIA. "SYNTHESIS AND OLIGOSACCHARIDE PROCESSING OF NORMAL AND ALTERED IMMUNOGLOBULIN M DURING B-CELL DIFFERENTIATION (GLYCOPROTEIN, GLYCOPEPTIDE, MUTANT, CARBOHYDRATE, ASPARAGINE-LINKED)." Diss., The University of Arizona, 1985. http://hdl.handle.net/10150/187906.

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Glycoproteins play a key role in cellular growth and differentiation. In order to study glycoprotein biosynthesis and processing, we have chosen the murine Immunoglobulin M (IgM) system as a model. Our system utilizes hybridoma, lymphoma and plasmacytoma cell lines which synthesize intracellular, membrane-bound and secreted IgM. Each type of IgM is synthesized during a specific phase of B-cell differentiation. We have examined the kinetics of IgM synthesis and processing in cells at each developmental stage. The rate of synthesis of membrane-bound and soluble IgM are different. Characteristic rates for membrane versus soluble IgM may be dependent on the extent of oligosaccharide processing. The membrane-bound IgM contains more high-mannose oligosaccharide than does the secreted product. In addition, we have begun to determine how protein structural requirements can affect final glycosylation patterns on the glycoprotein. Two cell lines were studied which secreted smaller than normal IgM heavy chains in tissue culture. One cell line studied (208) contains one glycosylation site, while another (562) retains three sites on the molecule synthesized in tissue culture. Studies performed on these cell lines in tissue culture indicate greater processing of the oligosaccharides on these mutant IgM molecules when compared to the parental cell line (PC700). Studies on the 208 IgM molecules synthesized in the mouse and purified from ascites fluid confirm these results. Upon injection into the mouse, the 562 cell line reverts to produce protein and carbohydrate structures characteristic of the parental cell line. Studies on the 562 protein purified from ascites fluid illustrate the need for more precisely defined cell lines and genetic engineering for the study of altered protein structures.

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9

Chan, Chun-yu. "Mass spectrometric analysis of selected glycoproteins." Thesis, Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B3147942X.

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10

Perry, J. Jefferson P. "Structural studies of cell surface glycoproteins." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368608.

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11

Edwards, Cathryn M. "Mucus glycoproteins in the diverted colorectum." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365830.

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12

Sage, Karen Anne. "The synthesis of glycopeptides and glycoproteins." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360466.

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13

Gupta, G. "Artificial lectins : biomimetic ligands for glycoproteins." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599789.

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This thesis describes the rational design and synthesis of selective and sterilisable biomimetic ligands for the resolution of glycosylated and carbohydrate depleted protein forms. Based on the principles of natural carbohydrate recognition, a putative library of 196 glycoprotein-binding ligands was designed and synthesised by solid phase combinatorial approaches. A preparative affinity chromatography screening assay against a well-characterised complex-type model glycoprotein, helped identify a triazine-based acyclic immobilised ligand 11/11, comprising bis-benzylamine substituents as the 'lead' ligand. The carbohydrate recognising potential of the 'lead' ligand was revealed by reduced binding of periodate oxidised model glycoprotein, and by 'sharp' elution profiles achieved with borate buffer eluents. Specific elution and competitive binding experiments of bound glycoprotein against free sugars showed that the monosaccharide specificity of the immobilised ligand was in the order mannoside>glucoside>galactoside. The ability of immobilised ligand 11/11 to bind several well-characterised oligomannose-, complex- and hybrid-type glycoproteins, and quantitatively elute them with mannoside confirmed its hexose sugar selectivity. Resolution of protein glycoforms was demonstrated by the ability of immobilised ligand 11/11 to retain and selectively elute the glycosylated form of an oligomannose-type protein, whilst the carbohydrate deficient counterpart bound non-specifically and irreversibly to the ligand. The structural integrity of immobilised ligand 11/11 was established by a semi-solution synthetic strategy, whereby the intermediate products were characterised by TLC, 1H-NMR and MS. This work has demonstrated the development of a diastereo-selective, sterilisable, stable and regulatory authority compliant biomimetic ligand, capable of resolving protein glycoforms.

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14

Juhasz, Katalin. "Major glycoproteins of turkey rhinotracheitis virus." Thesis, University of Warwick, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283496.

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15

Bristow, Richard G. W. "Antibody recognition of HIV-1 glycoproteins." Thesis, Open University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315370.

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16

Stephenson-Brown, Alexander James. "Synthetic sensors for saccharides and glycoproteins." Thesis, University of Birmingham, 2015. http://etheses.bham.ac.uk//id/eprint/5728/.

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The sensing of biological compounds is of vital importance to the screening and diagnosis of disease. The importance of such assays is due to the correlation observed between the observed levels of biological compounds and diseases such as cancer and diabetes mellitus. Compounds such as sugars and proteins are included in this useful class of molecules which can be used to detect pathology. Currently the detection of these compounds is achieved through the use of other biologically derived molecules- typically antibodies and enzymes. However, sensors based on these compounds can be limited in terms of their stability and suitability. Therefore there is a constant drive for novel detection methods for such molecules. In this context, the aims of the work described herein, are to produce synthetic sensing systems for the selective detection of saccharides and glycoproteins. This work will use principles of nanotechnology and self-assembly to produce surface sensors which exploit the revisable interactions of boronic acids to bind compounds of interest, and which employ surface plasmon resonance spectroscopy to enable the label free reporting of these binding events.

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17

Wood, Sarah Louise. "Glycoproteins of the chromaffin granule membrane." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/19427.

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18

Demers, Audrey Gertrude. "Structural studies of glycoproteins in solution." Case Western Reserve University School of Graduate Studies / OhioLINK, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=case1054759177.

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19

Macmillan, Derek. "The synthesis of novel homogeneous glycoproteins." Thesis, University of Edinburgh, 1999. http://webex.lib.ed.ac.uk/abstracts/macmil01.pdf.

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20

Vasiliauskaite, Ieva. "Structural characterization of viral envelope glycoproteins." Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066507/document.

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Les glycoprotéines virales sont impliquées dans les deux principales étapes d’entrée des virus enveloppés dans leurs cellules hôtes : l’attachement des virus aux récepteurs cellulaires et la fusion des membranes virale et cellulaire. Je me suis d’abord attachée à l’étude structurale de la principale glycoprotéine, E2, de deux hépacivirus : la forme B du virus GB (GBV-B) et le virus de l’hépatite C (HCV). Mes tentatives de cristallisation de l’ectodomaine de la protéine E2 du GBV-B sont restées vaines, mais l’analyse des propriétés de ses fragments a suggéré un rôle de son extrémité C-terminale dans la liaison à son récepteur. En parallèle, j’ai co-cristallisé un peptide synthétique correspondant à la principale boucle de liaison de E2 à son récepteur, avec un fragment d’anticorps dirigé contre cette boucle. Etonnament, le peptide forme une hélice , en nette contradiction avec la conformation étendue adoptée dans un fragment du cœur de E2. Associé à des données biochimiques, cela suggère une flexibilité inattendue de cette région de l’ectodomaine d’E2. Dans un second temps, je me suis intéressée à la glycoprotéine F des baculovirus. J’ai résolu la structure du trimère d’un fragment tryptique de F dans sa conformation post-fusion. Cette structure a validé une prédiction selon laquelle la protéine F était une protéine de fusion de classe I homologue à celle des paramyxovirus. La protéine F des baculovirus est ainsi le premier exemple d’une protéine de fusion de classe I encodée par un virus à ADN. Mes résultats confortent donc l’hypothèse que toutes les protéines F ont un ancêtre commun et suggèrent un lien évolutif intéressant entre les virus à ADN, à ARN et leurs hôtes Viral glycoproteins are responsible for the two major steps in entry into host cells by enveloped viruses: 1) attachment to cellular receptor/s and 2) fusion of the viral and cellular membranes. My thesis concentrated first on the structural analysis of the major envelope glycoprotein E2 of two hepaciviruses: GB virus B (GBV-B) and hepatitis C virus (HCV). Crystallization of the GBV-B E2 ectodomain remained unsuccessful, but the characterization of truncated versions of E2 suggested an important role of its C-terminal moiety in receptor binding. In parallel, I co-crystallized a synthetic peptide mimicking HCV E2 with an antibody fragment directed against the major receptor-binding loop of E2 that is targeted by broadly neutralizing antibodies. The structure unexpectedly revealed an α-helical peptide conformation, which is in stark contrast to the extended conformation of this region observed in the structure of an E2 core fragment. Together with further biochemical evidence this suggests an unanticipated structural flexibility within this region in the context of the soluble E2 ectodomain. Secondly, I focused on the structural analysis of the baculovirus glycoprotein F. I determined the crystal structure of the post-fusion trimer of a trypsin-truncated F fragment. This structure confirmed previous predictions that baculovirus F protein adopts a class I fusion protein fold and is homologous to the paramyxovirus F protein. Baculovirus F is therefore the first class I fusion protein encoded by a DNA virus. My results support the hypothesis that F proteins may have a common ancestor and imply interesting evolutionary links between DNA and RNA viruses and their hosts

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21

Vasiliauskaite, Ieva. "Structural characterization of viral envelope glycoproteins." Electronic Thesis or Diss., Paris 6, 2014. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2014PA066507.pdf.

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Les glycoprotéines virales sont impliquées dans les deux principales étapes d’entrée des virus enveloppés dans leurs cellules hôtes : l’attachement des virus aux récepteurs cellulaires et la fusion des membranes virale et cellulaire. Je me suis d’abord attachée à l’étude structurale de la principale glycoprotéine, E2, de deux hépacivirus : la forme B du virus GB (GBV-B) et le virus de l’hépatite C (HCV). Mes tentatives de cristallisation de l’ectodomaine de la protéine E2 du GBV-B sont restées vaines, mais l’analyse des propriétés de ses fragments a suggéré un rôle de son extrémité C-terminale dans la liaison à son récepteur. En parallèle, j’ai co-cristallisé un peptide synthétique correspondant à la principale boucle de liaison de E2 à son récepteur, avec un fragment d’anticorps dirigé contre cette boucle. Etonnament, le peptide forme une hélice , en nette contradiction avec la conformation étendue adoptée dans un fragment du cœur de E2. Associé à des données biochimiques, cela suggère une flexibilité inattendue de cette région de l’ectodomaine d’E2. Dans un second temps, je me suis intéressée à la glycoprotéine F des baculovirus. J’ai résolu la structure du trimère d’un fragment tryptique de F dans sa conformation post-fusion. Cette structure a validé une prédiction selon laquelle la protéine F était une protéine de fusion de classe I homologue à celle des paramyxovirus. La protéine F des baculovirus est ainsi le premier exemple d’une protéine de fusion de classe I encodée par un virus à ADN. Mes résultats confortent donc l’hypothèse que toutes les protéines F ont un ancêtre commun et suggèrent un lien évolutif intéressant entre les virus à ADN, à ARN et leurs hôtes Viral glycoproteins are responsible for the two major steps in entry into host cells by enveloped viruses: 1) attachment to cellular receptor/s and 2) fusion of the viral and cellular membranes. My thesis concentrated first on the structural analysis of the major envelope glycoprotein E2 of two hepaciviruses: GB virus B (GBV-B) and hepatitis C virus (HCV). Crystallization of the GBV-B E2 ectodomain remained unsuccessful, but the characterization of truncated versions of E2 suggested an important role of its C-terminal moiety in receptor binding. In parallel, I co-crystallized a synthetic peptide mimicking HCV E2 with an antibody fragment directed against the major receptor-binding loop of E2 that is targeted by broadly neutralizing antibodies. The structure unexpectedly revealed an α-helical peptide conformation, which is in stark contrast to the extended conformation of this region observed in the structure of an E2 core fragment. Together with further biochemical evidence this suggests an unanticipated structural flexibility within this region in the context of the soluble E2 ectodomain. Secondly, I focused on the structural analysis of the baculovirus glycoprotein F. I determined the crystal structure of the post-fusion trimer of a trypsin-truncated F fragment. This structure confirmed previous predictions that baculovirus F protein adopts a class I fusion protein fold and is homologous to the paramyxovirus F protein. Baculovirus F is therefore the first class I fusion protein encoded by a DNA virus. My results support the hypothesis that F proteins may have a common ancestor and imply interesting evolutionary links between DNA and RNA viruses and their hosts

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22

Ritchie, Gayle E. "The glycosylation of viral envelope glycoproteins and the effect of glycosidase inhibitors on virus replication and glycoprotein properties." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.442908.

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23

Fyrner, Timmy. "Synthesis of Structures Related to Antifreeze Glycoproteins." Thesis, Linköping University, The Department of Physics, Chemistry and Biology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-11941.

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In this thesis, synthesis of structures related to antifreeze glycoproteins (AFGPs) are presented. Synthetic routes to a protected carbohydrate derivative, 2,3,4,6-tetra-O-benzyl-β-galactopyranosyl-(1→3)-2-deoxy-2-azido-4,6-di-O-benzyl-β-D-thio-1-galactopyranoside, and a tBu-Ala-Thr-Ala-Fmoc tripeptide, are described. These compounds are meant to be used in the assembly of AFGPs and analogues thereof. A Gal-GlcN disaccharide was synthesized via glycosylation between the donor, bromo-2-O-benzoyl-3,4,6-tri-O-benzyl-α-Dgalactopyranoside, and acceptor, ethyl 4,6-O-benzylidene-2-deoxy-2-N-phthalimido-β-D-1-thio-glucopyranoside, using silver triflate activation. Subsequent epimerization to a Gal-GalN disaccharide was achieved using Moffatt oxidation followed by L-selectride® reduction. The tripeptide was synthesized in a short and convenient manner using solid phase peptide synthesis with immobilized Fmoc-Ala on Wang® resins as starting point.

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24

Kramer, Holger. "Synthesis of Glycoproteins and C-linked Glycopeptides." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.487272.

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Protein glycosylation is of great importance in nature due to its significance towards protein folding and stability, cell-cell communication and immunology. This thesisÃ‚Â·reports strategies that allow the synthesis of glycoprotein mimics as single glycoforms. This has been achieved through a combination of site-directed mutagenesis with unnatural amino acid incorporation and subsequent orthogonal chemical modification. The method also extends to the preparation of differentially modified glycoprotein mimics by means of two mutually orthogonal modification reactions. In addition to that, the direct synthesis of unprotected C-linked glycopeptides by olefin cross metathesis was investigated. Unnatural functionality which can be modified in an orthogonal manner to all other functional groups occurring in proteins has been introduced using methionine analogue incorporation. By combining residue specific replacement with site-directed mutagenesis alkene, alkyne and azide functionalities were site-selectively installed on the protein surface. The chemical modification was exemplified using Cu(!) catalysed triazole formation on Azido homoalanine (Aha) and Homopropargyl glycine (Hpg) residues with glycosyl azides and. alkynyl glycosides as reaction partners. The formed protein conjugates are attractive mimics of naturally occurring glycoproteins. The unnatural triazole linkage has the advantages of high chemical stability and it is not expected to be susceptible towards enzymatic degradation. The natural enzymatic activity of the protein was found to be retained in both the mutant proteins containing unnatural amino acids as well as in the modified protein conjugates. In addition to retaining the natural function of the enzyme, new lectin-binding properties were conferred upon the glycoconjugates. Specificity and strength of lectin-binding were shown to be dependent on the nature of the conjugated glycan. A combination of disulfide formation using glyco-MTS reagents and triazole synthesis allowed the creation of the first artifical glycoprotein bearing two different carbohydrate structures. Differential modification using the abovemethods also allowed the synthesis of a mimic of P-selectin glycoprotein ligand as a biological probe. This demonstrated the utility of this approach not only towards conjugation of glycosides, but also in the mimicry of other posttranslational modifications such as tyrosine sulfation. The synthesis of vinyl glycine (vGly) from selenomethionine (SeMet) was investigated as a means of incorporation of this ~,'Y-unsaturated amino acid into proteins and peptides. Facile oxidation to the selenoxide was achieved using hydrogen peroxide. The selenoxide elimination step required thermal activation and was shown to depend on the reaction medium and on the addition of seleninic acid scavengers. Furthermore, the utility of olefin cross metathesis (CM) for the direct synthesis of unprotected C-linked glycopeptides was investigated. To this end a number of alkene containing model peptides and olefinic C-glycosides were synthesized.' For the peptide systems both vinyl glycine (vGly) and homoalyll glycine (Hag) were utilized. The CM reaction was tested with a view towards modification of biologically important macromolecules. Therefore polar protic reaction media were investigated. It could be shown that the phosphine-free 2nd generation Hoveyda-Grubbs catalyst is stable over extended periods of time in methanol as reaction solvent. Nevertheless, catalyst decomposition occurs in the presence of terminal alkenes. As a result, repeated addition of the catalyst is required to achieve conversion in the metathesis reaction. At the same time olefin isomerisation processes take place with increasing catalyst loading. A variety of reaction conditions were investigated in order to limit the undesired side reactions and promote the productive CM process.

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25

Späte, Anne-Katrin [Verfasser]. "Metabolic Engineering of Glycoproteins / Anne-Katrin Späte." Konstanz : Bibliothek der Universität Konstanz, 2016. http://d-nb.info/1114893870/34.

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26

Hemming, Richard John. "Radioautographical and biochemical studies on nucleoplasmic glycoproteins." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41298.

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EM radioautography was used to examine the tissue distribution of cells exhibiting nucleoplasmic labeling after being exposed to $ sp3$H-sugars or $ sp{35}$S-sulphate to indicate the general extent of the occurrence of nucleoplasmic glycoproteins within animal cells. The observation of some degree of such labeling in virtually all cells in tissues of three animal species suggests that nucleoplasmic glycoproteins are a common cellular feature. To better define the distribution and nature of the putative labeled nucleoplasmic glycoproteins, cultured cells were used as a model cell type for both quantitative EM radioautographic and biochemical studies. After exposure to $ sp3$H-sugars, all three lines of cultured cells examined exhibited significant nucleoplasmic reaction in which the euchromatin, heterochromatin and nucleoli were all labeled to some extent. Studies on isolated, envelope-depleted nuclei from myeloma cells confirmed that the molecules in the nucleoplasm itself were the source of the radioautographic reaction observed over nuclei. Biochemical analyses of fractions of isolated nuclei indicated that much of the label resided in nuclear matrix glycoproteins of different molecular weights. Lectin binding studies on nuclear matrix fractions revealed the presence of galactose, fucose, and/or sialic acid residues in proteins. Glycosidase experiments indicated that some but not all of these glycoproteins had N-linked sidechains.

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27

Krishna, Sudhir. "T cell recognition of HSV-1 glycoproteins." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317944.

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28

Smyth, Edward. "Raman optical activity of proteins and glycoproteins." Thesis, University of Glasgow, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312130.

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29

Field, Mark C. "Structural studies on oligosaccharides from mammalian glycoproteins." Thesis, University of Oxford, 1989. http://ora.ox.ac.uk/objects/uuid:163fb9d7-43b7-4347-ab81-ce3cb87cb3f9.

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30

Snowden, B. W. "Structural and functional studies of herpesvirus glycoproteins." Thesis, University of Leeds, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355709.

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31

Fielding, Adel Kay. "Targeting fusogenic retroviral glycoproteins by ligand display." Thesis, University of London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322305.

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32

Spring, F. A. "Surface glycoproteins of normal and leukaemic leucocytes." Thesis, University of Bristol, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370674.

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33

Zeng, Chenhui. "Structure determination of glycoproteins by mass spectrometry." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/40167.

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34

Graham, Richard Peter. "Purification of glycoproteins from herpes simplex virus." Master's thesis, University of Cape Town, 1985. http://hdl.handle.net/11427/25694.

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The aim of this work was to purify type-specific glycoproteins from herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) for diagnostic use. The most likely candidate for a type-specific glycoprotein of HSV-1 is glycoprotein C (gC), although it has recently been shown to contain some type-common antigenic determinants. HSV-1 and HSV-2 were produced in BHK-21 cells and labelled with either (³H)-glucosamine ((³H)-gln) or a mixture of (¹⁴C)-amino acids ((¹⁴C)-aa). Analysis of the radiolabelled products by analytical sodium dodecyl sulphate polyacrylamide gel electrophoresis (SOS-PAGE) and autoradiography revealed that in the HSV-1 infected cells the radiolabelled components were incorporated into viral specific proteins only, whereas in the HSV-2 infected cells they were incorporated into host cell proteins as well as viral proteins. Preparative polyacrylamide gel electrophoresis (Prep-PAGE) was used as an initial step in separating HSV-1 infected cell proteins labelled with (³H)-gln. Two cycles of Prep-PAGE were sufficient to produce solutions containing either glycoprotein B ( gB) or glyco- protein C (gC), which were free of other HSV-1 glycoproteins. However, these solutions still contained a number of non-glycosylated proteins. Two different techniques were utilized to remove the non-glycosylated proteins from the glycoprotein solutions. Hydroxylapatite (HAUltrogel) chromatography in the presence of sodium dodecyl sulphate (SDS-HTP) did not separate the different HSV-1 glycoproteins and was not satisfactory for removing the non-glycosylated proteins. Gel-bound lectin affinity chromatography using wheat germ lectin and Helix pomatia lectin was not successful in purifying the glycoproteins because the glycoproteins which bound to the lectins could not be eluted under normal conditions. Difficulties encountered in eluting the HSV-1 glycoproteins from the lectins may have been due to the sodium dodecyl sulphate (SOS) in which the proteins were solubilized. For this reason, the gelbound lectin affinity chromatography was repeated using HSV-1 membrane proteins solubilized in a non-ionic detergent, Triton X-100. Using material prepared in this way, several HSV-1 glycoproteins were bound by wheat germ lectin and eluted under normal conditions to yield glycoproteins which were purified with respect to nonglycosylated proteins.

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35

Soby, Lynn Margaret. "Structure and viscoelasticity of proteoglycans and glycoproteins." Case Western Reserve University School of Graduate Studies / OhioLINK, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=case1059058747.

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36

Wang, Siyao. "Total Synthesis of Homogeneous Glycopeptides and Glycoproteins." Thesis, The University of Sydney, 2018. http://hdl.handle.net/2123/18329.

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Glycosylation is a ubiquitous post-translational modification of proteins. Access to pure glycoproteins is extremely challenging owing to the enzymatic glycosylation process which is not templated and, as such, affords heterogeneous mixtures of glycoforms. Chemical synthesis provides an attractive avenue to access homogeneously glycosylated peptides and proteins, thus providing a means to elucidate the role of specific glycan modifications on structure and function. This thesis outlined the development of a number of novel synthetic methods to access biologically important glycopeptides and glycoproteins in pure form. Examples would include the synthesis of an unusual glycan modification of arginine which is present on key proteins from pathogenic bacteria and the synthesis of a library of homogenously glycosylated chemokines and cytokines. The results from these studies would inform new anti-infective and anti-inflammatory strategies in the future.

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D'Souza, Yvonne. "Glycoproteins of drusen and drusen-like lesions." Thesis, University of Manchester, 2008. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489005.

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38

Bill, Roslyn M. "A study towards the chemical glycosylation of recombinant human erythroprotein." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240573.

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39

Byth, Katharine Fiona. "The targeted disruption of the CD45 gene in transgenic mice." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336538.

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40

Tomlinson, Michael Graham. "Structure and function of the leukocyte and surface antigens CD53 and CD37." Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308502.

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41

Lam, Ka-wai, and 林嘉維. "Glycodelin-A as a modulator of trophoblast invasion." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45598964.

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42

Kemp, Pauline Anne. "The glycosylation of human alpha-1-antitrypsin expressed in transgenic mouse milk." Thesis, University of Kent, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298167.

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43

Runswick, Sarah Kay. "Expression of laminin in the developing central nervous system of the chick embryo." Thesis, University of Liverpool, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295823.

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44

Haston, Jennifer Louise. "The inhibition of type II collagen fibril formation in rheumatoid arthritis." Thesis, University of Strathclyde, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248329.

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45

Malloy, Andrew Robert. "Atomic force microscopy studies of glycophorin A-BRAC 30 antigen-antibody interactions." Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247173.

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46

Molesworth, Sara J. I. M. "Expression and assembly of the Epstein-Barr virus (EBV) gp85, gp25 and gp42 fusion complex in the baculovirus system." Thesis, University of Bristol, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361142.

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47

Dee, Valerie Murielle. "Multiple forms of human complement factor H." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670272.

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48

Cheng, Chi-keung. "Structural organization of the mouse testin gene and characterization of its promoter sequence." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22424763.

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49

Perrine, Cynthia L. "Profiling Glycosyltransferase Peptide Substrate Specificities: Studies on ppGalNAc T1, T2, T10, and T-synthase That Initiate Mucin-Type O-Glycosylation." Cleveland, Ohio : Case Western Reserve University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=case1253046997.

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Thesis(Ph.D.)--Case Western Reserve University, 2009 Title from PDF (viewed on 2009-12-30) Department of Chemistry Includes abstract Includes bibliographical references and appendices Available online via the OhioLINK ETD Center

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50

Sabry, Zaki Tlep Sahar. "Identification of the molecular origins of disease in a cohort of patients with suspected congenital disorders of glycosylation (CDG)." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066715/document.

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Contexte : Les désordres congénitaux de la glycosylation (CDGs) sont des maladies rares dues à des mutations dans des gènes codant pour des protéines de la biosynthèse des glycoconjugués. Les CDGs présentent avec des glycoprotéines sériques hypoglycosylées avec un spectre clinique large. Le diagnostic moléculaire des CDG est important dans le cadre du diagnostic prénatal et du développement de stratégies thérapeutiques. Objectif : Déterminer les mutations causales dans une cohorte de cas suspects de CDG. Deux cas ont fait l’objet d’explorations biochimiques afin de comprendre les conséquences des mutations et d’envisager des stratégies thérapeutiques. Sujets/méthodes : Des explorations biochimiques sur des fibroblastes cutanés d’une cohorte de patients présentant des signes cliniques suggérant un CDG et hypglycosylation des protéines sérique. Résultats et conclusions: Le premier patient présentait une maladie multisystémique sévère. Des mutations affectant le gène codant pour la dehydrodolichol diphosphate synthase (DHDDS) ont été trouvées. Une activité diminuée la DHDDS était accompagnée de la diminution du dolichol phosphate. Ce patient est le premier cas de DHDDS-CDG présentant une atteinte multi-viscérale. Dans une deuxième étude deux siblings présentaient une thrombopénie associée à des atteintes neurologiques. Une mutation bi-allélique dans le gène codant pour le transporteur golgien du CMP-acide sialique (SLC35A1) associé avec une hypoglycosylation des protéines sériques a été détectée. Des profils anormaux des glycosphingolipides ont été mis en évidence et supplémentation des cellules de patient par de l’acide sialique a augmenté la biosynthèse des gangliosides Background: Congenital disorders of glycosylation (CDGs) are rare inherited diseases caused by mutations in genes required for glycoconjugate biosynthesis. CDG clinical presentations range from monosystemic to multiorgan failure. Often these diseases are diagnosed biochemically by the presence of hypoglycosylated serum proteins. Molecular diagnosis of CDG is crucial for both antenatal diagnostics and development of treatment strategies. Aims: To determine the molecular origins of disease in suspected CDG patients. Two cases were chosen for more extended biochemical explorations in order to investigate the consequences of the mutations and possible treatment strategies. Subjects/Methods: Biochemical explorations of skin biopsy fibroblasts from a cohort of patients presenting with signs suggestive of CDG, and serum protein hypoglycosylation. Results and conclusions: In the first study, a patient presented with multisystemic disease suggesting CDG. Fibroblasts revealed both truncated dolichol-linked oligosaccharides and polymannose-type N-glycans. Mutations in the dehydrodolichol diphosphate synthase (DHDDS) gene were found as well as low DHDDS activity and dolichol phosphate levels. As previous cases of DHDDS-CDG present with retinitis pigmentosa only, we describe the first case of a CDG syndrome associated with mutations in DHDDS. In the second study, two siblings presented with thrombocytopenia and CNS signs. A biallelic mutation in the CMP-sialic acid transporter gene (SLC35A1) was associated with hyposialylated serum glycoproteins. Altered glycosphingolipid profiles were seen and sialic acid supplementation of patient cells increased the appearance of gangliosides

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