Dissertationen zum Thema „Gene patterning“
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Sauers, Daniel J. „Light directed gene patterning using photocaged doxycycline /“. Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 196 p, 2010. http://proquest.umi.com/pqdweb?did=1993328811&sid=6&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Der volle Inhalt der QuelleMellick, Albert S. Jr, und n/a. „Tissue Specific Gene Expression Patterning and Carcinogenesis“. Griffith University. School of Health Science, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20041102.114313.
Der volle Inhalt der QuelleMellick, Albert S. Jr. „Tissue Specific Gene Expression Patterning and Carcinogenesis“. Thesis, Griffith University, 2004. http://hdl.handle.net/10072/365189.
Der volle Inhalt der QuelleThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Health Sciences
Faculty of Health Sciences
Full Text
Blader, Patrick. „Analysis of gene expression during early development of the zebrafish, Brachydanio rerio“. Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282415.
Der volle Inhalt der QuelleBoehm, Christian Reiner. „Gene expression control for synthetic patterning of bacterial populations and plants“. Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/267842.
Der volle Inhalt der QuelleJackman, William R. „Segmentation and molecular patterning of the amphioxus hindbrain, pharynx, and somites /“. view abstract or download file of text, 2000. http://wwwlib.umi.com/cr/uoregon/fullcit?p9998038.
Der volle Inhalt der QuelleTypescript. Includes vita and abstract. Includes bibliographical references (leaves 96-105). Also available for download via the World Wide Web; free to University of Oregon users.
Buxton, Paul Graeme. „Embryonic roles for the slug regulatory gene in hindbrain regulation and limb patterning“. Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266133.
Der volle Inhalt der QuelleMcKnight, Kristen Dawn. „Gene expression profiling reveals novel attributes of the mouse definitive endoderm“. Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/3431.
Der volle Inhalt der QuelleYamamoto, Mako. „The transformation suppressor gene Reck is required for postaxial patterning in mouse forelimbs“. Kyoto University, 2012. http://hdl.handle.net/2433/158062.
Der volle Inhalt der QuelleHidalgo-Downing, Alicia. „Molecular cloning of patched and analysis of its role in intrasegmental patterning in D. melanogaster“. Thesis, University of Oxford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258158.
Der volle Inhalt der QuelleAsh, David Michael Dobens Leonard L. „A dominant negative allele of the bunched gene blocks bunched function during tissue patterning“. Diss., UMK access, 2004.
Den vollen Inhalt der Quelle finden"A thesis in cellular and molecular biology." Typescript. Advisor: Leonard L. Dobens. Vita. Title from "catalog record" of the print edition Description based on contents viewed Feb. 22, 2006. Includes bibliographical references (leaves 51-58). Online version of the print edition.
Fernandes, Jolene Sabrina Rothenberg Ellen V. Sternberg Paul W. „Dissection of gene regulatory networks underlying patterning and morphogenesis in the C. elegans vulva /“. Diss., Pasadena, Calif. : California Institute of Technology, 2007. http://resolver.caltech.edu/CaltechETD:etd-05242007-164706.
Der volle Inhalt der QuelleNulsen, Candice Renee. „The development and evolution of arthropod appendages: Modulations of a limb patterning gene network“. Diss., The University of Arizona, 2000. http://hdl.handle.net/10150/289164.
Der volle Inhalt der QuelleCrease, Devanand John. „Gene induction and patterning in the early Xenopus embryo by molecules with long range activity“. Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624106.
Der volle Inhalt der QuelleAndreu, Cervera Abraham. „Role of the ciliopathy gene Ftm/Rpgrip1l and primary cilia in forebrain patterning and morphogenesis“. Electronic Thesis or Diss., Sorbonne université, 2018. http://www.theses.fr/2018SORUS223.
Der volle Inhalt der QuellePrimary cilia are essential for central nervous system development. In the mouse, they play a critical role in patterning the spinal cord and telencephalon via the regulation of Hedgehog/Gli signaling. However, despite the frequent disruption of this signaling pathway in human forebrain malformations, the role of primary cilia in forebrain morphogenesis has been little investigated outside the telencephalon. Here we studied development of the diencephalon, hypothalamus and eyes in mutant mice in which the Ftm/Rgprip1l ciliopathy gene is disrupted. At the end of gestation, Ftm-/- fetuses displayed anophthalmia, a reduction of the ventral hypothalamus and a disorganization of diencephalic nuclei and axonal tracts. In Ftm-/- embryos, we found that the ventral forebrain structures and the rostral thalamus were missing. Optic vesicles formed but lacked the optic cups. We analyzed the molecular causes of these defects. In Ftm-/- embryos, Sonic hedgehog (Shh) expression was lost in the ventral forebrain but maintained in the zona limitans intrathalamica (ZLI), the mid-diencephalic organizer. In the diencephalon, Gli activity was dampened in regions adjacent to the Shh-expressing ZLI but displayed a higher Hh-independent ground level in the other regions. Our data uncover a complex role of cilia in development of the diencephalon, hypothalamus and eyes via the region-specific control of the ratio of activator and repressor forms of the Gli transcription factors. They call for a closer examination of forebrain defects in severe ciliopathies and for a search for ciliopathy genes as modifiers in other human conditions with forebrain defects
Van, Leen Eric. „On the morphogenesis of the D. melanogaster pupa : a study on gene patterning and tissue folding“. Electronic Thesis or Diss., Sorbonne université, 2020. http://www.theses.fr/2020SORUS387.
Der volle Inhalt der QuelleIn order to achieve complex shapes during development, multicellular organisms need to coordinate cellular behaviors to form complex and functional organs. Identifying genes that are expressed in patterns that correlate with cellular processes is therefore primordial. Using the dorsal epithelium (the notum) of drosophila pupa as a model, my thesis aimed at uncovering the molecular mechanisms which control the spatial regulation of morphogenesis at the cell and tissue scale. First, I developed spatial transcriptomics which enabled the identification of new expression patterns involved in notum morphogenesis. Second, I developed, in collaboration with the imaging platform of Institut Curie, Rotating Sample Confocal Microscopy. Using this technique, I was able to simultaneously observe the morphogenesis of the notum, hinge and wing blade. This enabled the discovery of a new morphogenetic movement in the notum between 45-50hAPF. My results suggest that this extensive folding and elongation of the notum is independent of folding in the wing. Furthermore, I demonstrated that the expression of serine proteases regulate the attachment of the tissue to the cuticle which triggers the onset of the folding and determines the final shape of the tissue. Overall, this work increases our understanding of the spatial regulation of morphogenesis and contributes to the knowledge on how the extracellular matrix can regulate tissue shape
Goea, Laura Elena. „Regulatory interactions involved in pronephros development and patterning“. Electronic Thesis or Diss., Sorbonne université, 2018. http://www.theses.fr/2018SORUS576.
Der volle Inhalt der QuelleVertebrate organisms have evolved various mechanisms to control the spatial and temporal patterns of gene expression during growth, differentiation and development. It has become increasingly obvious that the regulatory frameworks are highly dependent on the transcriptional regulation in metazoan organism. The vertebrate kidney develops as three spatially and temporally distinct excretory systems with increasing complexity: the pronephros, the mesonephros, and, finally, the adult kidney or the metanephros (Saxen, 1987). In amphibians, the pronephros is the functional kidney of the tadpole. In Xenopus laevis, it includes a pair of single, large, non-integrated nephron, on each side of the embryo. It can be subdivided into three distinct domains: the glomus, where the blood is filtered into the coelomic cavity, the tubules, where selective re-absorption and secretion is taking place, and the connecting tubule, which carries the waste the cloaca. The pronephric kidney is segmented into distinct domains of the mammalian nephron, as evidenced by the differential expression of several membrane transporter genes (Raciti et al., 2008; Zhou and Vize, 2004). In addition, the Xenopus renal primordium represents a unique system to study gene network organization due to its relative simplicity and the increasing number of genetic regulators identified as necessary for its development.In vertebrates, the transcription factors Pax8 and Pax2 are crucial regulators of kidney development. A large set of studies performed in mouse, zebrafish and Xenopus have demonstrated their requirement for the establishment of the renal lineage, along with metanephric kidney induction, branching morphogenesis and nephron differentiation. Yet, the mechanisms by which they operate and the genes they regulate are still elusive. In Xenopus, loss of Pax8 leads to a complete absence of pronephric tubule, due to a defect in kidney field organization. In an attempt to identify Pax8 downstream targets during renal specification, we compared the microarray screen profiles of Pax8-deficient pronephric cells to the wildtype’s and identified several deregulated genes, including hnf1b, evi1 and mnx1. Morpholino-mediated knockdown experiments evidenced that Pax8 has indeed an essential role in the onset of evi1 and mnx1 expression and is sufficient to activate evi1 expression in ectodermal animal cap cells, as previously shown for hnf1b. Using in silico approaches, we identified an evolutionary conserved non-coding sequence (CNS1) located ~30kb upstream hnf1b gene locus, encompassing a putative Pax8 recognition motif (BS1). Electrophoretic mobility shift assay (EMSA) confirmed that this novel CNS1 directly interacts with Pax8. Using transfection assays in several kidney-derived cell lines, we demonstrate that luciferase activity driven by CNS1 was strongly up-regulated in response to either endogenous or exogenously added Pax8 and that this up-regulation is dependent on the presence of an intact Pax8-binding site. These findings suggest that Pax8 may regulate hnf1b1 expression through specific binding to the hnf1b CNS1, which may clarify the potential mechanism of Pax8 and hnf1b in nephrogenesis. Gene expression profile analysis revealed that evi1, hnf1b and mnx1 are expressed in a subset of the pax8 domain and in a specific region of the pronephric field in accordance with their known role in nephron patterning. We explored the potential cross-regulation between these transcription factors since it could contribute to establish their expression domain. Loss and gain-of-function experiments point to a genetic regulation between mnx1 and evi1, while no relationship can be observed between the other members. We view our results as a start toward building an eventually larger network of interactions between numerous directly and indirectly regulated molecular pathways that mediate specification and patterning of renal cells
Karam, Sana. „Mechanisms of pattern formation in the developing cerebellum : role for Eph receptor gene family /“. Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/10557.
Der volle Inhalt der QuellePahari, Shankar, und University of Lethbridge Faculty of Arts and Science. „The arabidopsis gene Grassy, is required for auxin transport and patterning of leaf vein, shoot and root“. Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2008, 2008. http://hdl.handle.net/10133/670.
Der volle Inhalt der Quellexii, 76 leaves : ill. ; 29 cm.
Ng, Medard Hein Tsoeng. „Genetic and molecular analyses of nubbin, a gene involved in proximal-distal patterning of the Drosophila wing“. Thesis, Open University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309862.
Der volle Inhalt der QuelleHeppert, Jennifer K. „Evolution of TOO MANY MOUTHS and stomatal patterning mechanisms in the monocot Dioscorea bulbifera“. Honors in the Major Thesis, University of Central Florida, 2009. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1273.
Der volle Inhalt der QuelleBachelors
Sciences
Biology
Brannon, Mark K. „Wnt pathway-mediated transcriptional regulation of the Xenopus dorsoanterior organizing gene siamois /“. Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/9256.
Der volle Inhalt der QuelleBehesti, Isfahani Hourinaz. „An analysis of the regulation of dorso-ventral patterning and T-box gene function in early eye development“. Thesis, University College London (University of London), 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433906.
Der volle Inhalt der QuelleWallbank, Richard William Roy. „Structure and function of the regulatory regions of pannier, a gene involved in the bristle patterning of Drosophilidae“. Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610369.
Der volle Inhalt der QuelleSjödal, My. „Specification of the lens and olfactory placodes and dorsoventral patterning of the telencephalon /“. Umeå : Univ, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1347.
Der volle Inhalt der QuelleWahl, Jacquelyn Marie Bell. „Genetics of merle patterning in the domestic dog and gene transcript profiling and immunobiology of dermatomyositis in the shetland sheepdog“. Thesis, [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2840.
Der volle Inhalt der QuellePersson, Madelen. „The role of transcriptional repression in Shh signalling and patterning of the ventral neural tube /“. Stockholm : Karolinska institutet, 2004. http://diss.kib.ki.se/2004/91-7349-834-3/.
Der volle Inhalt der QuelleAragón, Manresa Ferran. „The role of vHnf1 and Fgf Signaling in the caudal Hindbrain Patterning“. Doctoral thesis, Universitat Pompeu Fabra, 2008. http://hdl.handle.net/10803/7147.
Der volle Inhalt der QuellevHnf1 es un dels primers factors de transcripció expressats en el rombencèfal. En aquest projecte s'ha estudiat el paper de vHnf1 així com la implicació de la senyalització FGF en la regionalització del rombencèfal caudal del embrió de pollet. Els resultats mostren que vHnf1 s'expressa tempranament en el rombencèfal amb un límit rostral d'expressió coincident amb la frontera prospectiva entre els rombomers 4 i 5. Experiments de sobrexpressió mostren que vHnf1 es capaç de conferir caràcter caudal a rombomers rostrals tot induint Krox20 in MafB i reprimint Hoxb1 a r4. Les induccions de Krox20 i MafB resultaren ésser dependents de la senyalització per FGFs. Sorprenentment, els nostres resultats també mostren que vHnf1 indueix fortament la expressió de Fgf3. És més, anàlisis per RT-PCRs semiquantitatives demostraren que aquesta inducció es molt ràpida suggerint que Fgf3 és directament regulat per vHnf1. En aquest projecte també es presenta l'anàlisi dels perfils d'expressió d'alguns gens del grup de "sinexpressió" dels FGFs en el rombencéfal caudal. Finalment es determina que la senyalització FGF funciona a través de la via intracel·lular Ras-MAPK en el procés de regionalització del rombencéfal caudal sense implicació de la via PI3K-Akt.
Aquests resultats ofereixen nova informació sobre els mecanismes moleculars implicats en la regionalització del rombencèfal caudal en vertebrats. De manera interessant aquests resultats posen de manifest certes diferencies en els mecanismes de regulació que operen en la regionalització del rombencéfal de diferents especies.
During early embryonic development of chordates, the hindbrain, which is the caudalmost brain vesicle, is transiently organized along the AP axis in a series of segments called rhombomeres (r). This segmental organization serves as scaffold for several structures that develop within the hindbrain in repeated patterns. Each rhombomere has a molecular identity given by a specific combination of gene expression. Rhombomeric identity is the result of a progressively refined patterning that involves the interplay of different cell signaling pathways and rhombomere-specific transcription factors.
In the present project the role of vHnf1, one of the earliest transcription factors expressed in the hindbrain, and its interplay with FGF signaling has been analyzed during the chick embryo development. The results show that vHnf1 is very early expressed in the chick neuroepithelium with a sharp boundary of expression coinciding with the presumptive r4/r5 interrhombomeric boundary. Gain-of-function experiments demonstrated that vHnf1 is able to confer partial caudal character to rostral rhombomeres through the mediation of FGF signaling. We also analyzed the expression of genes of the FGF synexpression group in the caudal hindbrain. Finally, we determined that the role of FGF signaling in regulating the caudal rhombomeric markers Krox20 and MafB is mediated through the Ras-ERK1/2 intracellular pathway.
The results of this project provide new information about the molecular mechanisms involved in patterning the vertebrate caudal hindbrain. Interestingly, while requirement of vHnf1 and FGF signaling for caudal hindbrain patterning is an evolutionary conserved feature, the ways by which FGF signals are regulated during this process differ across species.
Chen, Hui-Min. „A More Accessible Drosophila Genome to Study Fly CNS Development: A Dissertation“. eScholarship@UMMS, 2015. http://escholarship.umassmed.edu/gsbs_diss/758.
Der volle Inhalt der QuelleBarbosa, Spinola Carla Maria. „Characterization of the use of AAVs as tools for in utero gene delivery and implications for the study of role of Sonic Hedgehog signaling in the differentiation and patterning of the cochlear epithelium“. Electronic Thesis or Diss., Sorbonne université, 2023. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2023SORUS408.pdf.
Der volle Inhalt der QuelleThe cochlea is the auditory sensory organ of mammals. It is a long spiral-shaped organ that decomposes complex sounds into distinct frequency components analyzed at different positions along its longitudinal axis (length). This frequency-position organization of the cochlea is supported by its morphological and mechanical properties that gradually vary along its longitudinal axis, also called basal-to-apical axis. Past studies showed that the Sonic Hedgehog (Shh) signaling pathway controls the establishment of the basal-to-apical axis of the cochlea, its size, and the differentiation of its auditory sensory cells. Yet, the time windows during which Shh controls these distinct processes during embryonic development are not well defined. It’s also not clear how morphological gradations arise along the basal-to-apical axis of the cochlea, and how Shh signaling contributes to these processes. My thesis project falls within the scope of these questions, and my objectives were (i) to characterize the activity of the Shh pathway within the developing cochlear neurosensory epithelium and (ii) to develop tools to manipulate gene expression temporally and spatially in the developing cochlea, at embryonic stages, with a primary focus on Shh signaling. To characterize Shh signaling activity, I first imaged whole mount cochlea expressing the Gli-reporter gene Tg(GBS-GFP) at embryonic days E13.5 and E14.5, but I couldn’t detect any specific GFP signal within the epithelium, suggesting that Shh signaling might be too low to be detected by this approach. Yet some GFP positive cells were present in the underlying mesenchyme. I next performed RNAscope in sections of whole head embryos (from E12.5 to E15.5), using probes targeting Shh pathway components (Gli transcription factors, Patched1 receptor, and Sonic Hedgehog). Overall, these genes are expressed in the sensory epithelium and surrounding tissues. Remarkably, Patched1 shows a graded expression, with highest expression at the cochlear apex at later stages (E15.5), nearest to the known spiral ganglion source of Shh, suggesting a modulation of Shh signaling by the epithelium. In parallel, I characterized two adeno-associated viral (AAV) vectors as tools for manipulating gene expression in the developing sensory epithelia of the inner ear. The AAV-PHP.eB and AAV-DJ serotypes were known to transduce sensory cells and supporting cells, respectively, with a high efficiency at postnatal stages, but their transduction rate was not characterized at embryonic stages. My first challenge was to develop the surgical procedure and practice to routinely perform in utero injections into the inner ear, from E13.5. By quantifying the transduction rate of AAV-PHP.eB at 48h, 72h and ~5 days post-injection, I found comparable transduction rates in the right and left inner ears, a higher transduction rate at the base than at the apex of the cochlea, and overall, a higher transduction rate at ~5 days post-injection. Injecting AAV-PHP.eB later at E14.5 also increased the transduction rate. As for AAV-DJ, the transduction was efficient only when the injection was performed after E15.5. These results suggest that the transduction efficiency of these viral vectors increases parallel to the maturation of cells from the base to the apex. In addition, more than 3 days are necessary to obtain a high expression of the reporter gene. My work revealed the expression pattern of the major Shh-pathway components within the developing neurosensory epithelium from E12.5 to E15.5. My work also established the temporal windows suitable for an efficient transduction of cochlear cell by the AAV-PHP.eB and AAV-DJ serotypes. This viral approach appears suited for manipulating gene expression from late embryonic stages on. For earlier embryonic stages, other conditional approaches should be considered including other viral vectors or genetics (CreERT2 -lox system)
John, Alphy. „Propriétés subcellulaires et dynamique à l'échelle de l'embryon gouvernant la morphogenèse“. Electronic Thesis or Diss., Université Côte d'Azur, 2021. http://theses.univ-cotedazur.fr/2021COAZ6017.
Der volle Inhalt der QuelleMorphogenesis is the process of reshaping single-cell zygotes to the final form of a developed animal. Embryonic gene patterning systems determine the body axes and lay down the spatiotemporal specification coordinates for cells. Gene patterning systems also affect the organization of cytoskeletal components in order to drive tissue morphogenesis. While much work was done to understand how AP and DV patterning independently control morphogenesis, little is known on how cross-patterning functions. We use the Drosophila embryo as a model system and focus on the process of tissue folding, a process that is vital for the animal since folding defects can impair neurulation in vertebrates and gastrulation in all animals which are organized into the three germ layers. Past work has shown that an actomyosin meshwork spanning the apical-medial side of prospective mesoderm cells and under the control of the embryo DV patterning plays a key role in mesoderm invagination. Nevertheless, both experimental and theoretical pieces of evidence have argued against apical constriction being the sole mechanism driving invagination. In this study, I have uncovered a lateral cell junctional network under the control of both AP and DV patterning. This contractile network generates tension along the apical-basal axis and within the tissue plane, 10-15 μm inside the mesoderm epithelium initiating lateral cell intercalation. Lateral forces in mesoderm cells seem to play a multivalent role in both driving mesoderm extension and invagination. Finally, by implementing 4D multi-view light-sheet imaging, infra-red femtosecond ablation to perturb the cytoskeleton, and optogenetics to synthetically control tissue morphology, this work shines new light on the origin and functions of a novel mechanism responsible for coordinated tissue elongation and folding
Moreno, González Eduardo. „Characterization of the Hox patterning genes in acoel flatworms“. Doctoral thesis, Universitat de Barcelona, 2010. http://hdl.handle.net/10803/1909.
Der volle Inhalt der QuelleFinding the extant bilateral organism closest to the bilaterian ancestor is the first and necessary step to open new ways of analysis. Recent molecular phylogenies have convincingly shown that the acoel flatworms, traditionally classified within the turbellarian Platyhelminthes, are the sister group of the remaining Bilateria, branching out before the common ancestor of protostomes, and deuterostomes.
Hox and ParaHox genes encode for transcriptional regulators involved in the control the AP body axis in all bilateral animals. Hox genes are usually organized in clusters in Bilateria, which have been originated by means of several gene tandem duplications from an original ProtoHox gene. In addition, Hox genes show a collinear correspondence between gene order within the cluster and the body levels at which these genes are expressed.
On the contrary, in the phylogenetic sister group of Bilateria, the Cnidaria, Hox genes are not linked in a single cluster and do not seem to play a similar role for patterning the OA axis.
Since it is still unclear when in the evolutionary history of bilaterians the Hox system first conferred positional identity along the AP-axis, the comparative study of the patterning genes Hox and ParaHox in acoel flatworms, could be crucial to understand the origin of the Hox-ParaHox axial patterning system and how the morphological transition from radial to bilateral animals took place.
In this Thesis, we report on the cloning, genomic arrangement, and expression domains of Hox genes in the acoel species Symsagittifera roscoffensis. Three Hox genes were detected: one from each of the major groups of Hox genes, which are anterior, central, and posterior, named SrHox1, SrHox5 and SrHoxPost respectively. All acoel species studied to date contain the same minimal complement of three Hox genes and one Cdx ParaHox gene, suggesting that the last common bilaterian ancestor (or Urbilateria) had a simple Hox gene complement, composed of only 3 or 4 genes.
In bacterial artificial chromosome cloning, sequencing, and chromosomal fluorescence in situ hybridization, Hox genes were not observed as being clustered in a unique genomic region in S. roscoffensis. Nevertheless, despite its dispersion within the genome, Hox genes are expressed in nested domains along the AP axis in the juvenile worm. The basic set of Hox genes in acoels and their coarse nested spatial deployment might be the first indicators of the role of Hox genes in the evolution of bilateral symmetry and AP positional identity from a hypothetical radial ancestor.
In order to understand how the AP axis has been established over evolutionary time, the execution of functional analyses is essential. With this purpose, we have performed the knockdown of the posterior Hox, IpHoxPost, during the postembryonic development, regeneration and adulthood of the acoel species Isodiametra pulchra, using RNA interference technologies.
The analysis has been done for the first time in acoels, and we demonstrate that the functions of this gene are restricted to the posterior region of the animal, within the muscular and neural tissues. We conclude, therefore, that the posterior Hox genes were used to specify and maintain defined anatomical regions within the AP axis of animals since the beginning of bilaterian evolution.
"Caracterización de los genes Hox en el acelo Symsagittifera roscoffensis"
TEXTO:
Los genes Hox codifican factores de transcripción que regionalizan el eje antero-posterior durante el desarrollo embrionario en todos los animales bilaterales estudiados.
Los animales radiales (cnidarios y ctenóforos) poseen genes Hox, pero estos no desempeñan un rol similar al de sus homólogos en Bilateria, por lo que el sistema de regionalización Hox puede ser considerado una innovación de los Bilateria.
Recientes análisis filogenéticos han demostrado que Acoelomorpha (acelos y nemertodermátidos), un grupo de gusanos clasificados tradicionalmente como platelmintos, divergieron antes del último antecesor común de protóstomos y deuteróstomos.
En consecuencia, representan el grupo de organismos bilaterales idóneo para estudiar la evolución del sistema Hox entre cnidarios y bilaterales. Por este motivo, el objetivo principal de esta tesis ha sido analizar el sistema Hox en acelos.
Encontramos un complemento simple de 3 genes Hox en las 2 especies de acelos estudiadas: Symsagittifera roscoffensis e Isodiametra pulchra. Estos genes no están ligados en el genoma de S. roscoffensis pero se expresan de forma colinear durante el desarrollo postembrionario, lo que representa el primer ejemplo de expresión colinear de genes Hox en Bilateria, indicando que la colinearidad estuvo presente en el ancestro de todos los Bilateria.
Las funciones del Hox posterior fueron analizadas mediante RNA de interferencia en I. pulcra. El fenotipo knockdown indica que IpHoxPost está implicado en la regulación del establecimiento de las estructuras morfológicas en la parte posterior, especialmente de los músculos bucales y los situados alrededor de los aparatos copuladores; así como en el proceso de maduración de los huevos y la proliferación celular. Esto indica que el rol del Hox posterior en la regulación del desarrollo y diversificación del mesodermo postembrionario y la musculatura ha surgido tempranamente durante la evolución de los Bilateria.
de, Sousa Nunes Rita Matos Dias. „Search for endoderm genes involved in early patterning of vertebrates“. Thesis, University College London (University of London), 2004. http://discovery.ucl.ac.uk/1446642/.
Der volle Inhalt der QuelleKolm, Peggy J. (Peggy Jeannette). „Patterning of the posterior neurectoderm by labial-like Hox genes and retinoids“. Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/43468.
Der volle Inhalt der QuelleJames, Karen Elizabeth. „The role of Ras in dorsoventral patterning and morphogenesis, and the developmental mechanism of eggshell evolution in Drosophila /“. Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/10298.
Der volle Inhalt der QuelleHaddon, Catherine. „The development of the Zebrafish ear and a quest for genes involved in sensory patterning“. Thesis, Open University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363972.
Der volle Inhalt der QuelleGremski, Kristina. „Gynoecium patterning in Arabidopsis thaliana control of transmitting tract development by the HECATE genes /“. Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3221292.
Der volle Inhalt der QuelleTitle from first page of PDF file (viewed September 8, 2006). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references.
Sakamoto, Susumu. „Id genes are required for morphogenesis and cellular patterning in the developing mammalian cochlea“. Kyoto University, 2020. http://hdl.handle.net/2433/253482.
Der volle Inhalt der QuelleRaciti, Marilena. „Reprogramming fibroblasts to neural-stem-like cells by structured overexpression of pallial patterning genes“. Doctoral thesis, SISSA, 2012. http://hdl.handle.net/20.500.11767/3924.
Der volle Inhalt der QuelleFRANCHINI, EMANUELA. „ROLE OF ALOG FAMILY GENES IN INFLORESCENCE PATTERNING IN ORYZA SATIVA AND ARABIDOPSIS THALIANA“. Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/714283.
Der volle Inhalt der QuelleFetter, Karl Christian. „Natural Selection For Disease Resistance In Hybrid Poplars Targets Stomatal Patterning Traits And Regulatory Genes“. ScholarWorks @ UVM, 2019. https://scholarworks.uvm.edu/graddis/1162.
Der volle Inhalt der QuelleMILLER, LEIGH-ANNE DEBORAH. „SPATIAL-TEMPORAL EXPRESSION OF SONIC HEDGEHOG REGULATES GROWTH, PATTERNING AND BRANCHING MORPHOGENESIS OF THE EMBRYONIC MOUSE LUNG“. University of Cincinnati / OhioLINK, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1070490268.
Der volle Inhalt der QuelleAllan, Deborah M. „Retinoic acid and mouse development : identification of retinoic acid receptor target genes involved in axial patterning“. Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=38451.
Der volle Inhalt der QuelleA precise level of RA signaling is also required for proper specification of vertebral identity. Exposure to excess RA during the early stages of gastrulation results in posterior homeotic transformations of several vertebrae. These transformations are correlated with shifts in the anterior boundaries of Hox expression. In contrast, the loss of functional RARs leads to anterior vertebral transformations. Although retinoic acid response elements have been identified in Hox promoters, it is likely that RA regulates the expression of some Hox genes through intermediary factors. Cdx1 is a homeobox-containing transcription factor that influences vertebral patterning in a manner similar to RARs, and is directly regulated by RA in the mouse embryo. Analysis of an allelic series of RARgamma/Cdx1 null mutant mice demonstrated that Cdx1 and RARgamma act synergistically to pattern certain cervical vertebrae. In addition, Cdx1 is required for the full effect of RA treatment on the vertebral column. However, Cdx1 does not mediate all of the effects of RARgamma as the incidence of a thoracic to cervical vertebral transformation is significantly higher in RARgamma/Cdx1 double mutants as compared to either single null mouse.
Wu, Shan. „The roles of OVATE and other elongation genes in regulating proximal-distal patterning of tomato fruit“. The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437586702.
Der volle Inhalt der QuellePuli, Oorvashi Roy G. „Defective proventriculus (Dve), a Novel Role in Dorsal-Ventral Patterning of the Drosophila Eye“. University of Dayton / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1406732166.
Der volle Inhalt der QuelleNewton, J. Michael 1969. „Molecular studies of two genes, Agouti andextension, which determine pigment production and patterning in the domestic dog“. Diss., The University of Arizona, 1998. http://hdl.handle.net/10150/282613.
Der volle Inhalt der QuellePei, Zhenglei. „The role of Gsx homeobox genes in the specification and differentiation of mouse lateral ganglionic eminence progenitors“. University of Cincinnati / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1298324694.
Der volle Inhalt der QuelleChoy, Siu Wah. „Characterization of a transcriptional repressor complex and mab-21 interacting genes in male sensory ray patterning of C. elegans /“. View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202006%20CHOY.
Der volle Inhalt der QuelleSiemanowski, Janna [Verfasser], Gregor [Akademischer Betreuer] Bucher und Roland [Akademischer Betreuer] Dosch. „Identification and analysis of novel insect head patterning genes / Janna Siemanowski. Gutachter: Gregor Bucher ; Roland Dosch. Betreuer: Gregor Bucher“. Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1076911498/34.
Der volle Inhalt der QuelleHorng, Sam H. „Identification and functional characterization of two patterning genes, Zic4 and Ten_m3, in topographic map formation of the visual pathway“. Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/58371.
Der volle Inhalt der QuelleCataloged from PDF version of thesis.
Includes bibliographical references (p. 114-123).
A central feature of visual pathway development is its organization into retinotopic maps. The developmental process by which these maps form involves a transition from early patterning cues to arrays of axonal guidance factors allowing the relative order of retinotopic axons to be preserved. Mechanisms linking patterning molecules of early development to topographic wiring and subsequent functional responses are not well understood. In this thesis, I performed a microarray screen comparing gene expression in early visual and auditory regions of the thalamus in order to identify early patterning candidates with a potential role in visual pathway differentiation. Among the candidates enriched in the visual thalamus, the transcription factor, Zic4, was found to be expressed in gradients of the developing retina, lateral geniculate nucleus (LGN) and primary visual cortex (V 1). Mice lacking Zic4 exhibited a deficit in eye-specific patterning to the thalamus that was complementary to the phenotype seen in mice lacking Tenm3, a type II homophilic transmembrane receptor and transcriptional regulator. Using intrinsic signal optical imaging techniques, I characterized the functional properties of primary visual cortical retinotopic maps in Zic4 and Ten_m3 null mice and identified complementary changes in the ipsilateral representation of V1, as well as evidence for eye-specific mismatch in the cortical binocular zone. Additionally, complementary positional shifts in VI were found in these mutants identifying a bidirectional modulation of mapping mechanisms in the visual pathway.
(cont.) In order to test whether Zic4 and Ten_m3 interact in serial or parallel pathways, I analyzed the retinogeniculate and cortical maps in the combination mutant. The Ten_m3/Zic4 double null mouse exhibited a partial rescue of retinogeniculate mapping and a complete reversal of the cortical changes found in either mutant alone, suggesting that the two genes interact to modulate common downstream effectors in opposite directions. In sum, this thesis presents a gene microarray screen used to identify Zic4 as a novel visual patterning gene, characterizes its loss-of-function phenotype on retinotopic mapping in the thalamus and cortex, and studies its antagonistic interaction with Ten_m3, another visual pathway patterning gene with a complementary loss-of-function phenotype.
by Sam H. Horng.
Ph.D.