Auswahl der wissenschaftlichen Literatur zum Thema „FLIMM method“
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Zeitschriftenartikel zum Thema "FLIMM method"
Zhang, Beini, Liping Li, Yetao Lyu, Shuguang Chen, Lin Xu und Guanhua Chen. „A New Instrument Monitoring Method Based on Few-Shot Learning“. Applied Sciences 13, Nr. 8 (21.04.2023): 5185. http://dx.doi.org/10.3390/app13085185.
Der volle Inhalt der QuelleWang, Quan, Yahui Li, Dong Xiao, Zhenya Zang, Zi’ao Jiao, Yu Chen und David Day Uei Li. „Simple and Robust Deep Learning Approach for Fast Fluorescence Lifetime Imaging“. Sensors 22, Nr. 19 (26.09.2022): 7293. http://dx.doi.org/10.3390/s22197293.
Der volle Inhalt der QuelleNeedham, Sarah R., Laura C. Zanetti-Domingues, Kathrin M. Scherer, Michael Hirsch, Daniel J. Rolfe, Selene K. Roberts, Marisa L. Martin-Fernandez, David T. Clarke und Christopher J. Tynan. „Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution“. Biochemical Society Transactions 43, Nr. 3 (01.06.2015): 309–14. http://dx.doi.org/10.1042/bst20140318.
Der volle Inhalt der QuelleJi, Mingmei, Jiahui Zhong, Runzhe Xue, Wenhua Su, Yawei Kong, Yiyan Fei, Jiong Ma, Yulan Wang und Lan Mi. „Early Detection of Cervical Cancer by Fluorescence Lifetime Imaging Microscopy Combined with Unsupervised Machine Learning“. International Journal of Molecular Sciences 23, Nr. 19 (29.09.2022): 11476. http://dx.doi.org/10.3390/ijms231911476.
Der volle Inhalt der QuellePande, P., C. A. Trivedi und J. A. Jo. „Analysis of Fluorescence Lifetime Imaging Microscopy (FLIM) Data“. Methods of Information in Medicine 49, Nr. 05 (2010): 531–36. http://dx.doi.org/10.3414/me09-02-0046.
Der volle Inhalt der QuelleLiu, Guoying, Pengwei Li und Yun Zhang. „A Color Texture Image Segmentation Method Based on Fuzzy c-Means Clustering and Region-Level Markov Random Field Model“. Mathematical Problems in Engineering 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/240354.
Der volle Inhalt der QuelleXU, LINGLING, ZHONG-CHAO WEI, SHAOQUN ZENG und ZHEN-LI HUANG. „QUANTIFYING THE SHORT LIFETIME WITH TCSPC-FLIM: FIRST MOMENT VERSUS FITTING METHODS“. Journal of Innovative Optical Health Sciences 06, Nr. 04 (Oktober 2013): 1350030. http://dx.doi.org/10.1142/s1793545813500302.
Der volle Inhalt der QuelleJi, Chao, Xing Wang, Kai He, Yanhua Xue, Yahui Li, Liwei Xin, Wei Zhao, Jinshou Tian und Liang Sheng. „Compressed fluorescence lifetime imaging via combined TV-based and deep priors“. PLOS ONE 17, Nr. 8 (12.08.2022): e0271441. http://dx.doi.org/10.1371/journal.pone.0271441.
Der volle Inhalt der QuelleMikicin, Mirosław. „Relationships of attention and arousal are responsible for action in sports“. Biomedical Human Kinetics 14, Nr. 1 (01.01.2022): 229–35. http://dx.doi.org/10.2478/bhk-2022-0028.
Der volle Inhalt der QuelleAdhikari, Mou, Rola Houhou, Julian Hniopek und Thomas Bocklitz. „Review of Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis Using Machine Learning“. Journal of Experimental and Theoretical Analyses 1, Nr. 1 (21.09.2023): 44–63. http://dx.doi.org/10.3390/jeta1010004.
Der volle Inhalt der QuelleDissertationen zum Thema "FLIMM method"
Mendoza, Lopez Duvan Alexander. „Étude des phénomènes de piégeage et dépiégeage de charges par mesures de charge d'espace et de décharge photo-stimulée dans des films polymères minces pour le stockage d'énergie“. Electronic Thesis or Diss., Toulouse 3, 2023. http://www.theses.fr/2023TOU30364.
Der volle Inhalt der QuelleWith the development of the polymer film capacitor market, there is growing interest in the study of charge generation, transport and trapping phenomena within relatively thin dielectric films. Indeed, increased requirements imposed by high supply voltages or elevated temperature conditions favor the appearance of charges which, as they migrate through the material, can be trapped to create space charge. This charge trapping dynamic is susceptible to causing localized intensifications of the electric field, thereby engendering electromechanical stresses that may ultimately culminate in material failure. Consequently, it is imperative to investigate the nature and properties of traps found in polymer dielectrics. The aim is to enrich our understanding of these materials and enhance the reliability of the systems in which they are incorporated. Among the available experimental approaches for investigating the electrical behavior of polymers, those capable of providing specific insights into the energy states of traps remain relatively scarce. The most prevalent methods in this regard are probably the Thermally Stimulated Discharge (TSD) and Photo-Stimulated Discharge (PSD) techniques. The PSD method, which entails measuring discharge currents during exposure to light within the UV-visible spectrum, offers the capability to identify interactions between mobile or trapped charges and the energy of incident photons. This approach possesses an advantage over the TSD method, which relies on a temperature ramp, as it does not induce alterations or destruction of traps through thermal effects. However, it is worth noting that interpreting PSD spectra poses challenges, as the measured current may originate from diverse phenomena, including photogeneration of charges or photoinjection of carriers, all of which are likely to interact with the existing traps. In an attempt to elucidate the underlying mechanisms governing in photoinduced current, we propose to couple PSD measurements to space charge measurements. By implementing protocols that systematically manipulate polarization and irradiation parameters, changes in the density or position of charges will serve as valuable indicators of the origin of charges and the kinetics of their trapping and detrapping processes. To achieve this objective, we intend to employ the Light Intensity Modulation Method (LIMM), which is particularly well-suited for the investigation of thin films (with dimensions on the order of a few micrometers) and offers excellent spatial resolution in proximity to interfaces. It will also be an asset for studying electric field reinforcement effects, linked to the use of an interdigitated electrode for PSD measurement. The integration of LIMM and PSD methods within the same experimental setup, and their use in sequential measurement programs, offers the possibility of continuously monitoring trap filling, trap release by light irradiation, and exploring the relationships between applied light energy and trap characteristics. In fact, the measurement system demonstrates in an innovative approach that the reduction in space charge density after PSD measurements is directly attributed to the light disturbance itself, rather than being influenced by concurrent factors such as the time elapsed after the charge period or manipulations leading to charge release
Hosny, Neveen Amera. „Development of a non-invasive method to detect pericellular spatial oxygen gradients using FLIM“. Thesis, Queen Mary, University of London, 2011. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1262.
Der volle Inhalt der QuelleFERRI, Gianmarco. „A biophysical approach to the study of living β-cell“. Doctoral thesis, Scuola Normale Superiore, 2020. http://hdl.handle.net/11384/91107.
Der volle Inhalt der QuelleChakraborty, Sandeep, und 夏柏杉. „Fluorescence based methods (FLIM and FRET) to study the metabolic state of Parkinson’s disease in cell model and in vitro protein intractions“. Thesis, 2016. http://ndltd.ncl.edu.tw/handle/41738728403186503249.
Der volle Inhalt der Quelle國立陽明大學
生醫光電研究所
104
Fluorescence based spectroscopic and microscopic techniques have been widely used in the field of scientific research and medical diagnostics for their unique advantages, such as specificity, sensitivity, simplicity, and speed. Over the years, two of the most frequently used techniques based on fluorescence are Förster/fluorescence resonance energy transfer (FRET) spectroscopy (and microscopy) and fluorescence lifetime imaging microscopy (FLIM). Fluorescence lifetime imaging technique quantifies the average time a fluorophore remains in the excited state before descending to the ground state. This technique can easily distinguish two molecules with similar fluorescence emission bands, or the same molecule with different structural conformations based on their fluorescence lifetime. In this work, we applied two-photon fluorescence lifetime imaging microscopy (2P-FLIM) to observe two endogenous fluorescent molecules viz. reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavin adenine dinucleotide (FAD). NADH have longer lifetime when it binds to protein and free NADH has shorter lifetime. On the other hand, FAD has shorter lifetime when it binds to protein, while the free FAD has longer time. In this thesis, we exploited these properties of NADH and FAD to monitor the cellular metabolic state in Parkinson’s disease (PD) cellular model in terms of the cellular redox ratios NADH/NAD+ and FADH2/FAD via 2P-FLIM. NADH and FAD are two co-enzymes which take part in the ATP production of cells in the inner-mitochondrial membrane; we monitored via 2P-FLIM to map the cellular metabolism in PD. The cellular redox state can be interpreted in terms of the fluorescence lifetime components values of NADH and FAD, and also the relative contributions of free to protein-bound NADH (and FAD). Two-photon excitation was used for lifetime imaging of NADH and FAD, as it causes less photobleaching and yields higher cell viability. PD is a progressive neurological disorder due to the loss of dopaminergic neurons in substantia nigra of mid-brain region and several lines of evidence suggest that mitochondrial dysfunction is responsible for the disease pathology. In this work, PC12 cells were first treated with nerve growth factor (NGF) to differentiate it into neuronal cells which were further treated with 1-methyl-4-phenylpyridinium (MPP+) to establish the PD cellular model. A systematic FLIM data analysis showed a statistically significant (p < 0.001) decrease in the fluorescence lifetime of both free and protein-bound NADH, as well as free and protein-bound FAD in MPP+ treated cells. On the relative contributions of the free and protein-bound NADH and FAD to the life time, however, both the free NADH contribution and the corresponding protein-bound FAD contribution increased significantly (p < 0.001) in MPP+ treated cells, compared to control cells. These results, which indicate a shift in energy production in the MPP+ treated cells from oxidative phosphorylation towards anaerobic glycolysis, can potentially be used as cellular metabolic metrics to assess the condition of PD at the cellular level. In this thesis, we also developed an organic fluorophore based steady-state quantitative FRET assay with a new and modified algorithm to extract FRET emission signal. This method was further applied to quantify the interaction between leukocyte function-associated antigen-1(LFA-1) and intercellular adhesion molecule-1 (ICAM-1) in terms of the dissociation constant (Kd). The interaction between these two transmembrane proteins plays a significant role in cellular adhesion including the extravasation and inflammatory response of leukocytes, and also in the formation of immunological synapse. Moreover, the LFA-1/ICAM-1 interaction may serve as a potential therapeutic target for the treatment of several diseases as irregular expressions of LFA-1 or ICAM-1 or both may lead to autoimmune diseases, metastasis cancer, etc. In addition, we also developed the FRET assay into a screening platform to identify peptides and small molecules that inhibit the LFA-1/ICAM-1 interaction. For the FRET pair, we used Alexa Fluor 488-LFA-1 conjugate as the donor and Alexa Fluor 555-human recombinant ICAM-1 (D1-D2-Fc) as the acceptor. From our quantitative FRET analysis, the Kd between LFA-1 and D1-D2-Fc was determined to be 17.93 ± 1.34 nM. Both the Kd determination and screening assay were performed in a 96-well plate platform, providing the opportunity to develop it into a high-throughput assay. In future, these approaches can be further developed/optimized for the study of in vivo Parkinson’s disease pathogenesis and for small molecules based drug screening.
Buchteile zum Thema "FLIMM method"
Petre, Anca, Didier Marty-Dessus, Laurent Berquez und Jean-Luc Franceschi. „FLIMM and FLAMM Methods“. In Dielectric Materials for Electrical Engineering, 251–70. Hoboken, NJ USA: John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118557419.ch12.
Der volle Inhalt der QuelleKukk, Olga, Jeffrey Klarenbeek und Kees Jalink. „Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET Sensors“. In cAMP Signaling, 105–16. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2245-2_7.
Der volle Inhalt der QuelleMorton, Penny E., und Maddy Parsons. „Measuring FRET Using Time-Resolved FLIM“. In Methods in Molecular Biology, 403–13. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-207-6_27.
Der volle Inhalt der QuelleBonilla, Pedro Andrade, und Rebika Shrestha. „FLIM-FRET Protein-Protein Interaction Assay“. In Methods in Molecular Biology, 261–69. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3822-4_19.
Der volle Inhalt der QuelleYoo, Tae Yeon, und Daniel J. Needleman. „Studying Kinetochores In Vivo Using FLIM-FRET“. In Methods in Molecular Biology, 169–86. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3542-0_11.
Der volle Inhalt der QuelleWindgasse, Lukas, und Carsten Grashoff. „Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM“. In Methods in Molecular Biology, 221–37. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2851-5_15.
Der volle Inhalt der QuelleLuyben, Thomas T., Jayant Rai, Bingyue Zhou, Hang Li und Kenichi Okamoto. „Two-Photon FRET/FLIM Imaging of Cerebral Neurons“. In Methods in Molecular Biology, 33–43. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3810-1_4.
Der volle Inhalt der QuellePandzic, Elvis, Renee Whan und Alex Macmillan. „Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR“. In Methods in Molecular Biology, 257–83. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1843-1_20.
Der volle Inhalt der QuelleBecker, Wolfgang, Samuel Frere und Inna Slutsky. „Recording Ca++ Transients in Neurons by TCSPC FLIM“. In Advanced Optical Methods for Brain Imaging, 103–10. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-9020-2_5.
Der volle Inhalt der QuelleLionetti, Maria Chiara, und Caterina Anna Maria La Porta. „FLIM-FRET Investigation of Heterogeneous Huntingtin Aggregation in HeLa Cells“. In Methods in Molecular Biology, 595–604. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2597-2_36.
Der volle Inhalt der QuelleKonferenzberichte zum Thema "FLIMM method"
Pham, C. D., V. Griseri und L. Berquez. „Space charge distribution detection by FLIMM and PEA method on electron beam irradiated dielectric films“. In 2009 IEEE Conference on Electrical Insulation and Dielectric Phenomena (CEIDP). IEEE, 2009. http://dx.doi.org/10.1109/ceidp.2009.5377748.
Der volle Inhalt der QuelleCerqueira, Matheus A., und Alexandre X. Falcão. „User-Assisted Design of a Neural Network for Brain Tumor Segmentation“. In Anais Estendidos da Conference on Graphics, Patterns and Images. Sociedade Brasileira de Computação - SBC, 2023. http://dx.doi.org/10.5753/sibgrapi.est.2023.27455.
Der volle Inhalt der QuelleLeiter, Nina, Maximilian Wohlschläger und Martin Versen. „Frequency-domain fluorescence lifetime imaging as method to analyze wood structures“. In CLEO: Applications and Technology. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/cleo_at.2022.jw3a.19.
Der volle Inhalt der QuelleNguyen, T. X., S. Bouchareb, V. Griseri und L. Berquez. „Post-electronic irradiation measurements by PEA and FLIMM methods on dielectric films“. In 2011 IEEE Conference on Electrical Insulation and Dielectric Phenomena - (CEIDP 2011). IEEE, 2011. http://dx.doi.org/10.1109/ceidp.2011.6232780.
Der volle Inhalt der QuelleKanaani, Salar, Mohammad Sadegh Helfroush, Habibollah Danyali und Mohammad Ali Kazemi. „Segmentation of skin lesions using an improved FLICM method“. In 2017 7th International Conference on Computer and Knowledge Engineering (ICCKE). IEEE, 2017. http://dx.doi.org/10.1109/iccke.2017.8167919.
Der volle Inhalt der QuelleJunek, J., und K. Zidek. „FLIM via RATS Method Using Single Pixel Camera“. In 3D Image Acquisition and Display: Technology, Perception and Applications. Washington, D.C.: OSA, 2020. http://dx.doi.org/10.1364/3d.2020.jw5c.1.
Der volle Inhalt der QuelleAlfonso-Garcia, Alba, Lisanne Kraft, Xiagnan Zhou, Julien Bec, Laura Marcu, Dongguang Wei, Shiro Urayama und Asha Cogdill. „Colorectal Polyp Assessment with Label-Free Fluorescence Lifetime Imaging“. In Clinical and Translational Biophotonics. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/translational.2024.tm3b.2.
Der volle Inhalt der QuelleHuang, YuHeng, Huaixin Guo und Tangsheng Chen. „Thermal conductivity of SiN flims with different thicknesses by 3ω method“. In Sixth Symposium on Novel Photoelectronic Detection Technology and Application, herausgegeben von Huilin Jiang und Junhao Chu. SPIE, 2020. http://dx.doi.org/10.1117/12.2557726.
Der volle Inhalt der QuelleSchwarz, Jonas, Maximilian Wohlschläger, Nina Leiter, Veronika Auer, Michael Risse und Martin Versen. „Frequency Domain Fluorescence Lifetime Imaging Microscopy (FD-FLIM) analysis of Quercus robur samples for origin differentiation purposes“. In Applied Industrial Spectroscopy. Washington, D.C.: Optica Publishing Group, 2023. http://dx.doi.org/10.1364/ais.2023.jtu4a.10.
Der volle Inhalt der QuelleKomarova, A. D., S. D. Sinyushkina, A. M. Mozherov, I. S. Kritchenkov, S. P. Tunik, I. N. Druzhkova, V. I. Shcheslavskiy und M. V. Shirmanova. „IN VIVO STUDY OF THE OXYGEN AND METABOLIC STATUS OF TUMORS DURING ANTI-TUMOR THERAPY USING THE PLIM/FLIM METHOD“. In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-185.
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