Thematische Bibliographien / Fibre digestion

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Auswahl der wissenschaftlichen Literatur zum Thema „Fibre digestion“

Autor: Grafiati

Veröffentlicht am 27. Juni 2021

Zuletzt aktualisiert am 1. Februar 2022

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Zeitschriftenartikel zum Thema "Fibre digestion"

Suresh, Harsha, Vincent Ho und Jerry Zhou. „Rheological Characteristics of Soluble Fibres during Chemically Simulated Digestion and their Suitability for Gastroparesis Patients“. Nutrients 12, Nr. 8 (17.08.2020): 2479. http://dx.doi.org/10.3390/nu12082479.

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Dietary fibres are an integral part of a balanced diet. Consumption of a high-fibre diet confers many physiological and metabolic benefits. However, fibre is generally avoided by individuals with gastrointestinal motility disorders like gastroparesis due to increased likelihood of exacerbated symptoms. Low-viscosity soluble fibres have been identified as a possible source of fibre tolerable for these individuals. The aim of this study is to determine the rheological properties of 10 common commercially available soluble fibres in chemically simulated digestive conditions and evaluate their suitability for individuals with mild to moderate gastroparesis, a gastric motility disorder. Rheological testing under neutral condition (distilled water pH 7) and chemically simulated gastric digestion were evaluated to determine the yield point and relative viscosity of each fibre. Our results reveal two rheological categories of soluble fibres; pseudoplastic and dilatant. Simulated digestion was shown to significantly alter the yield-points of psyllium husk, iota-carrageenan, beta-glucan, apple-fibre pectin, and inulin. Gum Arabic and partially hydrolysed guar gum showed the lowest viscosities and were not affected under simulated digestion, characteristics that make them potential candidate fibres for patients with gastroparesis. Altogether, our results demonstrate that digestion can have a significant impact on fibre viscosity and should be taken into consideration when evaluating the suitability of fibres for patients with gastric motility disorders.

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Stanogias, George, und G. R. Pearcet. „The digestion of fibre by pigs“. British Journal of Nutrition 53, Nr. 3 (Mai 1985): 513–30. http://dx.doi.org/10.1079/bjn19850061.

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1. The effects of the amount and the type of dietary fibre on the apparent digestibility (AD) by growing pigs of neutral-detergent fibre (NDF) and NDF components, on nitrogen balance and on the rate of passage of digesta were studied using a semi-purified basal diet and fibre in the forms of soya-bean hulls, lupin (Lupinus sp.) hulls, pea (Pisum sativum) hulls, wheat bran, maize hulls, maize cobs, oat hulls and lucerne (Medicago sativa) stems.2. Both the amount and the type of dietary fibre significantly influenced the AD of dietary dry matter, N and energy. The AD of NDF and of NDF components was markedly affected by the type and the amount of fibre in the diet. The proportion of NDF digested ranged from 0.016 to 0.905, of cellulose from 0.026 to 0.931 and of hemicellulose from 0.010 to 0.999.3. N retention by the pigs ranged from 12.9 to 25.8 g/d and with some fibres there was a tendency towards increased N retention with increasing intakes of NDF.4. Rate of passage of digesta, expressed as the 50 and 95% excretion times of stained feed particles, ranged from 22.2 to 85.1 h and 40.0 to 117.1 h respectively. Large individual variations in rate of passage occurred but, in general, the rate of passage tended to increase with increasing intakes of NDF. No strong associations between the rate of passage of digesta and apparent digestibility of NDF components were observed.5. The results suggest that the extent of fibre digestibility depends predominantly on the origin of the fibre and to a lesser extent on the amount of fibre in the diet.

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Cazemier, Anne E., Huub J. M. Op den Camp, Johannes H. P. Hackstein und Godfried D. Vogels. „Fibre Digestion in Arthropods“. Comparative Biochemistry and Physiology Part A: Physiology 118, Nr. 1 (September 1997): 101–9. http://dx.doi.org/10.1016/s0300-9629(96)00443-4.

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Vong, M. H., und M. L. Stewart. „In vitro bacterial fermentation of tropical fruit fibres“. Beneficial Microbes 4, Nr. 3 (01.09.2013): 291–95. http://dx.doi.org/10.3920/bm2013.0006.

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Tropical fruits such as mango, papaya, pineapple and banana are rich sources of dietary fibre. However, few studies have examined the potential physiological effects of fibre from these tropical fruits. The aim of this study was to characterise the fermentability of dietary fibre found in banana, papaya, pineapple and mango as an estimate of the physiological effects of consuming these fruits. Freeze-dried fruit was subjected to in vitro digestion to remove digestible carbohydrates. Digestion residues were freeze-dried prior to fermentation. In vitro fermentation was carried for 24 h under anaerobic conditions to simulate conditions in the large intestine. Gas volume, pH and short chain fatty acids (SCFAs) concentration were measured at 0, 4, 8, 12, and 24 h. SCFAs were analysed by gas chromatography. There was no gas production from 0 to 8 h time points for all samples. Mango fibre resulted in more gas at 12 and 24 h than pineapple, papaya and banana fibres. The slurry pH was significantly lower for mango fibre at 12 and 24 h compared to other samples. Mango fibre resulted in significantly more propionate at 8 h compared to papaya and pineapple fibres. Butyrate concentrations were only significantly different at 4 h. At 24 h total and individual SCFA production did not differ among samples. All fruit fibres were fermentable, with mango fibre being the most rapidly fermented. Additional work is necessary to confirm a benefit on digestive health.

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Gilbert, Harry J., und Geoffrey P. Hazlewood. „Genetic modification of fibre digestion“. Proceedings of the Nutrition Society 50, Nr. 2 (01.08.1991): 173–86. http://dx.doi.org/10.1079/pns19910028.

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Boctor, Dana. „The role of dietary fibre and prebiotics in the paediatric diet“. Paediatrics & Child Health 25, Nr. 4 (Juni 2020): 263. http://dx.doi.org/10.1093/pch/pxaa032.

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Abstract Dietary fibres are resistant to digestion and absorption. Prebiotics are fermentable dietary fibres that confer health benefits through their effects on microbiome composition and activity. The range of physiological benefits from consuming dietary fibres is broad. Encouraging children to eat fibre-rich foods promotes a nutrient-dense diet. Introducing a variety of dietary fibre sources to young children helps establish future dietary choices and a more diverse intestinal microbiota. Low-fibre intake is associated with a higher prevalence of constipation and obesity.

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Dawson, Terence J., Peter J. Whitehead, Adam McLean, F. D. Fanning und William R. Dawson. „Digestive function in Australian magpie geese (Anseranas semipalmata)“. Australian Journal of Zoology 48, Nr. 3 (2000): 265. http://dx.doi.org/10.1071/zo00011.

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The Australian magpie goose (Anseranas semipalmata) is not really a ‘goose’ but an aberrant species representing the monotypic family Anseranatidae. It is herbivorous but its ability to utilise dietary fibre is uncertain. We examined digestive processes in tame birds fed natural forages in metabolism cages and in wild birds. An examination of the gross anatomy of the gut showed features similar to those in waterfowl of the family Anatidae, the true ducks and geese. In a total-collection feeding trial geese were fed either unhusked rice grain or fresh aquatic grass. The aquatic grass was high in fibre (neutral detergent fibre (NDF) was 74% of dry matter) and magpie geese could not maintain energy or nitrogen balance on this feed. The maintenance energy requirement of the caged magpie geese, as estimated on the rice diet, was 573 kJ kg–1 day–1, which was similar to that found for other species of geese. The maintenance nitrogen requirement was 0.44 g N kg–1 day–1 or 0.52 g N kg–0.75 day–1, which also was similar to the average value for birds. Fibre digestion on both diets was small, 19% and 27% of NDF for rice and grass respectively. Rates of passage of fibrous digesta through the gut of magpie geese varied with diet. The mean retention time for fibre was longer when feeding on the aquatic grass than on unhusked grain, 7.7 3.0 h v. 3.7 0.6 h Data from wild magpie geese clarified the process of digestion. The patterns of pH and short-chain fatty acids along the gut pointed to acid and enzymic digestion occurring in most of the tract, down to the ileocaecal junction. Fermentation appeared restricted to the caeca, rectum and cloaca, though, of note, the caeca contained little fibre, 5% NDF. Higher levels of fibre digestion were indicated in wild geese but fibre still was not a major contributor to the energy intake of these birds. The digestive capabilities of the magpie geese were considered in relation to their impact on the feeding and reproductive biology of these ‘geese’ in monsoonal, northern Australia

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Stanogias, George, und G. R. Pearce. „The digestion of fibre by pigs“. British Journal of Nutrition 53, Nr. 3 (Mai 1985): 531–36. http://dx.doi.org/10.1079/bjn19850062.

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1. The effect of including lupin (Lupinus sp.) hulls, maize cobs, wheat bran and lucerne (Medicago sutiva) stems in a basal fibre-free diet on the concentrations and the relative proportions of volatile fatty acids (VFA) in the proximal colon of pigs, 17–18 h after feeding, was studied.2. Concentrations of total VFA in the proximal colon increased with increasing levels of neutral-detergent fibre (NDF) intake, and this increase was highly dependent on the source of NDF in the diet.3. Molar proportions of the VFA were significantly affected by the level of NDF intake only in the cases of acetic and butyric acids, whereas the source of dietary NDF had a marked influence on the molar proportions of all acids.4. The results indicate that the extent of fermentative breakdown of fibre in the pig intestine can be influenced substantially by the type and the level of fibre in the diet.

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Lu, C. D., J. R. Kawas und O. G. Mahgoub. „Fibre digestion and utilization in goats“. Small Ruminant Research 60, Nr. 1-2 (Oktober 2005): 45–52. http://dx.doi.org/10.1016/j.smallrumres.2005.06.035.

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Stanogias, George, und G. R. Pearce. „The digestion of fibre by pigs“. British Journal of Nutrition 53, Nr. 3 (Mai 1985): 537–48. http://dx.doi.org/10.1079/bjn19850063.

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1. The aim of the study was to determine the relative effects of feeding growing pigs with graded amounts of neutral-detergent fibre (NDF) from various sources on the empty wet weight of segments of the pig gastrointestinal tract, on the weight and moisture content of their digesta, and on the pattern of digesta movement in them.2. Increased NDF intakes were associated with significantly higher wet weights of all gastrointestinal segments and increased lengths of the caecum. The lengths of both the small and the large intestines were unaffected by the ingestion by the pigs of different amounts of NDF from various sources. However, the caecum responded to these increased intakes of NDF by significant increases in length. The source of NDF in the diet was a factor that markedly influenced both the length and the weight of the distal colon. The nature of these increases in weight and length morphologically and their biological significance have not been determined.3. Despite some significant differences, neither the type nor the level of dietary NDF had any appreciable effect on the dry weight and on the proportion of dry matter (DM) of the contents in the stomach. As the level of NDF intake was increased, more undigested dietary material was found in all segments of the digestive tract of the pigs. The proportion of DM in the residues decreased progressively from the caecal contents to the contents of the distal colon. In most cases the degree to which the level of NDF intake affected the weight of the contents and the proportion of DM in them was highly dependent on the source of NDF in the diet.4. The distribution of the feed consumed in the morning and in the evening, as measured with stained feed particles and polyethylene beads, was extremely variable. It is concluded that prolonged intakes by pigs of diets containing high levels of fibre may lead to a hypertrophy and hence increased weight of segments of the gastrointestinal tract.

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Dissertationen zum Thema "Fibre digestion"

Stebbens, Helen Rose. „The digestion and utilisation of food fibre by growing pigs“. Thesis, University of Edinburgh, 1988. http://hdl.handle.net/1842/27458.

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A series of investigations were undertaken to provide information for compounders to consider when including fibrous foods in diets for growing pigs. The work involved measurements of nutrient digestibilities, growth trials and calorimetric studies. The digestibility of the neutral detergent fibre (NDF) fraction of a food was dependent on a number of factors including the source of fibre, the adaptation period and liveweight of the pig, and the protein and lipid contents of the diet. The level of addition of food fibre was not important in determining the digestibility of the NDF component of a fibre source, whereas an inverse relationship was found between the digestibility of nitrogen and energy, and the level of added food fibre. It was concluded that increasing the level of addition of food fibre is more important in determining the depressive effect on the digestibility of non-fibre components than its own. Rapid growth was achieved when young, weaned and growing pigs were given diets containing wheatfeed and sugar-beet pulp. A substantial part of the energy supplied was in the form of VFA produced by the fermentation of non-starch polysaccharides in the wheatfeed and sugar-beet pulp. A growth trial with diets containing sugar-beet pulp and maize revealed that fermented energy from beet pulp was used with an efficiency of 0.72 that of the energy of maize. The apparently digested energy arising from the fermentation of non-starch polysaccharides, therefore, does not correspond directly in terms of potential use to the animal with that obtained by the enzymic digestion of maize starch. A net energy value for sugar-beet pulp was also calculated. This work has shown that wheatfeed and sugar-beet pulp may be useful as dietary ingredients for growing pigs. However, growth rates may be slightly depressed due to a lower efficiency of utilisation of apparently digested energy from the fibrous fraction of the diet. This could lead to on farm problems associated with a decreased throughput eg overstocking. However, the use of a corrected digestible energy value and ileal digestibilities of amino acids for a fibrous food, when formulating rations, could lead to an improvement in the prediction in performance. As well as the nutritional aspects of feeding fibrous foods, there are also a number of non-nutritional factors which will influence the use of fibrous materials and these are discussed in the light of their practical implications for the feeding of fibrous foods to pigs.

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Key, Fiona Brigit. „Digestion and large intestinal fermentation of breads and haricot beans (Phaseolus vulgaris)“. Thesis, University of Newcastle Upon Tyne, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308980.

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Useni, Bilungi Alain. „Effect of exogenous fibrolytic enzymes on fibre and protein digestion in ruminant animals“. Thesis, Stellenbosch : University of Stellenbosch, 2011. http://hdl.handle.net/10019.1/6744.

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Thesis (MScAgric (Animal Sciences))--University of Stellenbosch, 2011.
Includes bibliography.
ENGLISH ABSTRACT: Forages are the main feed components in ruminant production systems for the reason that they are often the major source of energy available to the animal. However, only 10 to 35% of energy intake is available as net energy because the digestion of plant cell walls is not complete. This can significantly affect livestock performance and profits in production systems that use forages as a major source of nutrients of the diet. As a result of low and variable nutritive values of forage feedstuffs, attempts to improve ruminal fibre degradability have been an ongoing research topic. The use of exogenous fibrolytic enzymes (EFE) has been proposed as means to improve forage digestibility. Positive results with regard to rumen forage digestibility and other animal production traits have consequently been obtained due to increased rumen microbial activity following EFE addition in ruminant diets. Two EFE (Abo 374 and EFE 2) and one commercial yeast preparation were firstly identified and selected for their potential to improve the cumulative gas production (GP) at 24 hours of a range of feed substrates using the in vitro GP system as a screening step to identify the superior EFE products. The different feed substrates were lucerne hay, wheat straw, wheat straw treated with urea and a commercial concentrate diet. An in vitro experiment was undertaken on these four different substrates in order to evaluate the two EFE and the yeast preparation. This was to identify the most promising EFE capable of producing a significant effect on feed digestibility using organic matter digestibility (in vitro true digestibility) and fermentation characteristics (in vitro GP system). Results from the in vitro evaluation showed that EFE significantly enhanced in vitro DM degradability and GP profiles (P < 0.05). Abo 374 enzyme showed potential to increase in vitro microbial protein synthesis (MPS) of GP residues of the concentrate diet. In addition, no correlation was found between the in vitro MPS and the 48 hours cumulative GP of all the tested substrates (P < 0.05; R2 < 0.30). Treatments were found to increase in vitro MPS, feed degradability and the cumulative GP of different quality forages and the concentrate diet, with Abo 374 being the best treatment (P < 0.05). However in vitro responses of EFE were variable depending on the energy concentration and chemical composition of different substrates. Variation in MPS was mostly due to the low recovery of purine derivates with the purine laboratory analysis. On the basis of these results, Abo 374 was selected and consequently further tested in another in vitro and in situ trial using a mixed substrate of lucerne hay and wheat straw. Abo 374 significantly improved the cumulative GP, in vitro DM and NDF disappearance of the mixed substrate (P < 0.05). In addition, no correlation was found between the in vitro MPS and the cumulative GP at 48 hours (P = 0.68; R2 < 0.25). The in situ disappearance of feed nutrients (DM, NDF and CP) with Abo 374 was similar to the control. The lack of significance of disappearance was probably due to the small number of sheep used in the study and the relatively high coefficient of variation associated with measuring ruminal digestion. Abo 374 significantly increased the in situ MPS (P = 0.0088) of the mixed substrate of lucerne hay and wheat straw. Evidence of the increased MPS and both in vitro and in situ disappearance of DM and NDF resulted from the Abo 374 activity during either the pre-treatment or the digestion process. The addition of Abo 374 to the mixed substrate of lucerne hay and wheat straw appeared to have been beneficial for microbial colonization of feed particles as a result of the increased rumen activity. It could be speculated that the primary microbial colonization was thus initiated, leading to the release of digestion products that attract in return additional bacteria to the site of digestion. This EFE may be efficient to produce some beneficial depolymerisations of the surface structure of the plant material and the hydrolytic capacity of the rumen to improve microbial attachment and the feed digestibility thereafter. Therefore, the mechanism of action by which Abo 374 improved the feed digestion can be attributed to the increased microbial attachment, stimulation of the rumen microbial population and synergistic effects with hydrolases of ruminal micro-organisms. With regard to these findings, the addition of EFE in ruminant systems can improve the ruminal digestion of DM, NDF and CP to subsequently enhance the supply of the metabolizable protein to the small intestine. Key words: crude protein (CP), exogenous fibrolytic enzymes (EFE), dry matter (DM), gas production (GP), neutral detergent fibre (NDF), microbial protein synthesis (MPS).
AFRIKAANSE OPSOMMING: Ruvoere is die hoof-voerkomponent in herkouer produksiesisteme aangesien dit dikwels die vernaamstebron van energie aan herkouer is. Slegs 10 tot 35% van die energie-inname is beskikbaar as netto-enrgie, omdat die vertering van selwande onvolledig is. Dit kan die prestasie en profyt in produksiesisteme drasties beïnvloed waar ruvoere as ’n hoofbron van nutriënte in die dieet gebruik word. Aangesien die nutriëntwaarde van ruvoere laag is en baie varieer, is navorsing vir verbeterde ruminale veselvertering steeds ’n voorgesette onderwerp. Dit is voorgestel dat eksogeniese fibrolitese ensieme (EFE) gebruik kan word vir verbeterde ruvoervertering. Positiewe resultate in ruminale ruvoerverterig en ander diereproduksie-eienskappe, is verkry as gevolg van toenemende rumen mikrobiese aktiwiteit na EFE aanvulling in herkouerdiëte. Twee EFE’s (Abo 374 en EFE 2) en `n gisproduk is geïdentifiseer en geselekteer vir hul potensiaal om die kumulatiewe gasproduksie (GP) na 24 uur met ’n reeks voersubstrate te verbeter met die gebruik van die in vitro GP sisteem as seleksiemetode om die superieure EFE produkte te identifiseer. Die verskillende ruvoersubstrate was lusernhooi, koringstrooi, ureumbehandelde koringstrooi en ’n kommersiële konsentraatdieet. ’n In vitro eksperiment was onderneem om die vier verskillende substrate te gebruik om die twee EFE’s en gisproduk te evalueer. Hierdeur sou die belowendste EFE’s identifiseer kon word wat ’n betekenisvolle effek op ruvoervertering het. Die vertering van ruvoer sal bepaal word deur organiese materiaal vertering (in vitro ware vertering), asook fermentasie-eienskappe (in vitro GP sisteem). Resultate van die in vitro evaluering het getoon dat EFE’s in vitro DM degradering en GP profiele verbeter. Dit blyk dat die Abo 374 ensiem ’n potensiële toemame in in vitro mikrobiese proteïensintese (MPS), soos bepaal deur die GP oorblyfsels van konsentraat diëte, tot gevolg gehad het. Daar was geen korrelasie tussen die in vitro GP en MPS van al die proefsubstrate nie. Dit blyk dat die behandelings ’n toename in in vitro GP, MPS en ruvoerdegradeerbaarheid van lae kwaliteit ruvoer- en konsentraatdiëte gehad het, waar Abo 374 die beste behandeling was. Die in vitro reaksies van die EFE’s was egter wisselend, afhangende van die energiekonsentrasie en die chemiese samestelling van die verskillende substrate. Variasie van MPS was meestal as gevolg van die lae herwinning van purienderivate tydens die purienanalise. Op grond van dié resultate, is Abo 374 geselekteer om verdere toetse in ander in vitro en in situ proewe te doen. Die substraat wat gebruik is, was ’n 1:1 mengsel van lusernhooi en koringstrooi. Abo 374 het die kumulatiewe RP, in vitro DM en NBV verdwyning van die gemengde substraat verbeter. Boonop is geen korrelasie tussen die MPS en in vitro GP gevind nie. In situ verdwyning van DM, NBV en RP was hoër vir Abo 374, maar nie betekenisvol nie. Die gebrek aan betekenisvolle verdwynings mag die gevolg wees van die klein hoeveelheid skape wat in die proef gebruik is, asook die relatiewe hoë koëffisient van variasie wat gepaard gaan met die bepaling van ruminale vertering. Abo 374 het die in situ MPS betekenisvol verhoog. Verhoogde MPS en in vitro en in situ verdwyning van DM en NBV is waargeneemwaarskynlik as gevolg van die aktiwiteit van Abo 374 gedurende die voorafbehandeling óf die verterings proses. Die byvoeging van Abo 374 tot die gemengde substraat van lusernhooi en koringstrooi blyk om voordelig te wees vir mikrobiese kolonisering van voerpartikels as gevolg van ’n toename in rumenaktiwiteit. Die primêre mikrobiese kolonisering het waaarskynlik gelei tot die vrystelling van verteringsprodukte wat addisionele bakterieë na die plek van vertering lok. Die EFE mag geskik wees vir voordelige depolimerisasie op die oppervlakstruktuur van die plantmateriaal, asook verbeterde hidrolitiese kapasiteit van die rumen om sodoende mikrobiese aanhegting, asook ruvoervertering te verbeter. Dus, Abo 374 se meganisme van aksie wat verbeterde ruvoervertering tot gevolg het, kan toegeskryf word aan `n verhoogde mikrobiese aanhegting, stimulering van die rumen mikrobiese populasie en die sinergistiese effek met hidrolases van rumen mikroörganismes. Ten opsigte van die bevindings, kan die byvoeging van EFE in herkouersisteme ruminale vertering van DM, NBV en RP verbeter, wat dan daaropvolgend die dunderm met meer metaboliseerbare proteïn sal voorsien. Sleutelwoorde: eksogene fibrolitiese ensieme (EFE), droëmaterial (DM), ruproteïen (RP), neutraal bestande vesel (NBV), mikrobiese proteïensintese (MPS), gasproduksie (GP).

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Anelich, Claudia Bruna. „Association between grinding energy and in-vitro neutral detergent fibre digestion kinetics in forages“. Diss., University of Pretoria, 2017. http://hdl.handle.net/2263/63233.

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Through the measurement of grinding energy, the possibility exists to predict forage fragility as related to the chemical composition of forages. It is also possible to predict a potential relationship between forage fragility and 240 hour in vitro neutral detergent fibre digestibility (uNDF240). These results could lead to improved predictions of particle size reduction, animal chewing activity and energy usage during the process of chewing. Physically effective fibre (peNDF) is a key component of many nutritional models used to predict the effect of forage particle size on cow chewing response. Chewing activity is a response which reflects the chemical and physical properties of feeds, including intrinsic fragility. Forage fragility, or the ease of particle size reduction during chewing, has been said to be similar among different sources of NDF, when attempting to estimate peNDF. However, different NDF sources with similar particle sizes can elicit ariable chewing responses and this variation has serious implications for nutritional models which use peNDF values. This variation has led to numerous inaccuracies in the system; therefore factors affecting peNDF particularly forage fragility, need to be better understood as forage fragility may be closely linked to NDF digestibility. Therefore, in this study, a possible association between forage fragility and short term or long term in vitro NDF digestibility (ivNDFd) was investigated. In order to investigate the possibility of predicting an association between forage fragility and in vitro NDF digestion, a total of 35 forage samples from three forage species were collected from 25 different locations. Forage species included commonly used fibre sources in ruminant nutrition in South Africa, namely Medicago sativa, Maize silage and Eragrostis curvula. The forage samples were analysed for numerous chemical components, as well as 6-, 12-, 18-, 24-, 36-, 48-, 72-, 96-, 120-, 240-h ivNDFd and rate of NDF digestion (kd). The 240-h ivNDFd was used to estimate indigestible NDF (iNDF). Particle size distributions were measured for all forage samples. Dried samples were pre-cut with a knife mill fitted with a 2 cm screen, after which particle size distributions were determined for each sample using a Retsch sieve shaker. For the measurement of grinding energy, 10 g duplicates of the 2 cm milled samples were milled with an ultra-centrifugal mill, fitted with a 1 mm screen. During the grinding process, energy usage of the specific mill was measured using a data logger with corresponding computer software and energy transducer. Energy measurements were reported as J/g sample on dry matter (DM) basis. The 1 mm samples were then used for determining particle size distribution again, in order to analyse change in particle distribution for each forage sample. The results of this study showed, according to the final models, that initial particle size (IPS), final particle size (FPS), cellulose and undigested NDF at 6 hours digestion (uNDF6) explain most of the variation in forage fragility. All of these variables can be associated with a decrease in forage fragility, due to an increase in energy usage during grinding with an increase in any of the aforementioned components. Upon adding species as a variable that could influence forage fragility, it could be seen that an interaction between M. sativa and FPS can be associated with a decrease in forage fragility, whereas an interaction between maize silage and FPS can be associated with an increase in forage fragility, due to a decrease in energy usage during grinding with an increase in this interaction. From the simple associations and correlations, it was evident that kd can be associated with increased forage fragility, as there was a decrease in energy usage during grinding with an increase in this parameter. Further correlations and/or linear associations indicate that NDF, acid detergent fibre (ADF), uNDF18, uNDF24, uNDF36 and uNDF48 can possibly be associated with a decrease in forage fragility, due to an increase in energy usage during grinding with an increase in any one of these variables. It would be expected that acid detergent lignin (ADL) is also associated with decreased forage fragility; however, this can only be assumed as the results for the effect of lignin on forage fragility are non-significant in this study. The use of grinding energy has the potential to be a practical and useful measure to predict forage fragility. However, the relative contribution of physical factors such as original particle size, particle shape, surface area, morphology and a multitude of chemical factors toward the fragility of forages is difficult to predict. Additional research is needed on the prediction of forage fragility and the possible relationship between forage fragility and NDF digestion and which factors influence this concept, before it can be incorporated as a meaningful and accurate input into nutritional models such as the National Research Council (NRC) and the Cornell Net Carbohydrate and Protein System (CNCPS).
Dissertation (MSc)--University of Pretoria, 2017.
Animal and Wildlife Sciences
MSc (Agric)
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Colombatto, Dario. „Use of enzymes to improve fibre utilisation in ruminants : a biochemical and in vitro rumen degradation assessment“. Thesis, University of Reading, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340076.

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Rallis, Ilias. „Intelligent chromatic fibre optic sensors and monitoring systems for enhancing useful by-products from anaerobic digestion“. Thesis, University of Liverpool, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.417968.

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Chikunya, Sifelani. „Microbial protein synthesis and fibre digestion in the rumen in relation to the form of supplementary nitrogen“. Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624398.

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Dickner-Ouellet, Laurie. „Optimisation de l'usage des coproduits dans l'alimentation des porcs en croissance : impact du type de fibre et de la xylanase sur la digestion des nutriments“. Master's thesis, Université Laval, 2018. http://hdl.handle.net/20.500.11794/29576.

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L’augmentation du coût des ingrédients de base des aliments pour porcs a permis à certains ingrédients alternatifs de se tailler une place sur les marchés nord-américains de l’alimentation animale. En effet, leur utilisation judicieuse en production porcine peut représenter un atout économique important. Toutefois, l’usage d’ingrédients alternatifs est limité dans les aliments pour porcs, notamment à cause leur effet parfois négatif sur la qualité de la carcasse ainsi que de leur teneur élevée en fibres alimentaires, peu valorisées par les monogastriques. Ce projet avait comme objectif de mieux comprendre l’impact de l’usage des ingrédients alternatifs disponibles dans l’Est du Canada afin de diminuer les coûts d’alimentation, sans modifier les performances de croissance chez le porc. Plus précisément, le projet visait à évaluer l’impact de différents types de fibres (solubles et insolubles) et de la supplémentation en xylanase, une enzyme exogène, sur la digestibilité iléale apparente des composants nutritionnels ainsi que sur les performances de croissance et la composition corporelle chez le porc en croissance-finition. Pour ce faire, deux expérimentations ont été réalisées. La première était un essai de digestibilité réalisé à l’aide de porcs canulés chez lesquels la digestibilité iléale apparente a été mesurée pour différents aliments variant en leur teneur et leur type de fibres, et selon l’addition ou non de xylanase. La seconde expérimentation consistait en un essai de croissance chez le porc en finition afin de mesurer ces mêmes traitements sur les performances de croissance, la composition corporelle et la qualité du gras. Les résultats obtenus montrent que les niveaux élevés de fibres alimentaires utilisés n’ont pas eu d’effets majeurs sur la digestibilité iléale apparente des nutriments et de l’énergie, sur la composition corporelle et sur les performances de croissance des animaux. De plus, l’ajout de xylanase dans les aliments a eu peu d’effets sur les critères mesurés

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Selvin, David. „Regulation of Myoplasmic Ca2+ During Fatigue in KATP Channel Deficient FDB Muscle Fibres“. Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/26174.

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It is known that muscles that lack KATP channel activity generate much greater unstimulated [Ca2+]i and force than normal muscles during fatigue. The increase in unstimulated force in KATP channel deficient muscles is abolished by a partial inhibition of L-type Ca2+ channels, suggesting that it is due to a Ca2+ influx through L-type Ca2+ channels and a subsequent increased myoplasmic Ca2+. However, there is also evidence that the increase in resting force is abolished by NAC, a ROS scavenger. The objective of this study was to reconcile these observations by studying the hypothesis that “the increase in resting [Ca2+]i during fatigue in KATP channel deficient muscles starts with an excess Ca2+ influx through L-type Ca2+ channels, followed by an excess ROS production that causes a further increase in resting [Ca2+]i”. To test the hypothesis, single FDB fibres were fatigued with one tetanic contraction/sec for 180 sec. KATP channel deficient fibres were obtained i) by exposing wild type muscle fibers to glibenclamide, a KATP channel blocker and ii) by using fibres from Kir6.2-/- mice, which are null mice for the Kir6.2 gene that encodes for the protein forming the channel pore. Verapamil, a L-type Ca2+ channel blocker, applied at 1 μM, significantly reduced resting [Ca2+]i during fatigue in glibenclamide-exposed wild type fibres. NAC (1 mM) also reduced resting [Ca2+]i in glibenclamide-exposed muscles. The results suggest that the increase in resting [Ca2+]i during fatigue in KATP channel deficient FDB fibres is due to an influx through L-type Ca2+ channels, and an excess ROS production.

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Wilfart, Aurélie. „Digestion et transit digestif chez le porc en croissance : influence de la composition de l’aliment“. Rennes, Agrocampus, 2007. http://www.theses.fr/2007NSARB178.

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La mise a point de modèles de digestion nécessite de caractériser le transit et la digestibilité des nutriments dans les différents segments digestifs ces paramètres dépendent de l’animal et de la composition de l’aliment. Le premier objectif du travail de thèse était d’évaluer les conséquences d’une modification de la composition de l’aliment sur la digestibilité des nutriments et le transit dans les segments digestifs. Le deuxième était de quantifier les cinétiques d’hydrolyses des matières premières dans ces mêmes organes. Le temps de séjour moyen des digesta était de 1 heure dans l’estomac, 4 heures dans l’intestin grêle et 38 heures dans le gros intestin. L’augmentation de la teneur en fibres dans l’aliment réduit le temps de transit dans l’intestin grêle etle gros intestin diminue la digestibilité fécale des nutriments. Les cinétiques d’hydrolyse ont révélé que l’amidon était essentiellement hydrolysé dans l’intestin grêle et que l’hydrolyse dépendant de la matière première
To develop mechanistic models of digestion, digesta transit and nutrient digestibility have to be quantified in different segments of the gastrointestinal tract. The processes are affected by the animal and feed composition. The objectives of the thesis were to quantify the consequences of a modification inf feed composition on nutrient digestibility and on passage kinetics in the gut, and quantifying the kinetics of hydrolysis (solubilisation) of main dietary constituents. The mean retention time averaged 1, 4 and 38 h in the stomach, small intestine, and large intestine respectively. Increasing the insoluble fibre content in the diet decreased the mean retention time in the small intestine and the large intestine and decreases faecal digestibility of nutrients. Kinetics of hydrolysis indicated that starch was essentially hydrolyzed in the large intestine. The extent and rate of hydrolysis depended on the raw material

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Bücher zum Thema "Fibre digestion"

Karppinen, Sirpa. Dietary fibre components of rye bran and their fermentation in vitro. Espoo [Finland]: VTT Technical Research Centre of Finland, 2003.

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Virginia, Hopkins, Hrsg. Dr. Earl Mindell's what you should know about fiber and digestion. New Canaan, Conn: Keats, 1997.

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Digestive health and nondigestible carbohydrates. Ames, Iowa: Wiley-Blackwell, 2011.

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Paeschke, Teri M., Teri M. Paeschke und William R. Aimutis. Digestive health and nondigestible carbohydrates. Ames, Iowa: Wiley-Blackwell, 2011.

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Mulligan, Finbar J. The in vivo digestibility of ruminant feed ingredients. Dublin: University College Dublin, 1997.

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Kenny, Maria J. Prediction of in vivo digestibility of ruminant feed ingredients by laboratory methods. Dublin: University College Dublin, 1997.

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Aimutis, William R., und Teresa M. Paeschke. Nondigestible Carbohydrates and Digestive Health. Wiley & Sons, Incorporated, John, 2010.

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Aimutis, William R., und Teresa M. Paeschke. Nondigestible Carbohydrates and Digestive Health. Wiley & Sons, Incorporated, John, 2011.

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Aimutis, William R., und Teresa M. Paeschke. Nondigestible Carbohydrates and Digestive Health. Wiley & Sons, Incorporated, John, 2011.

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(Editor), J. R. Bennett, und R. H. Hunt (Editor), Hrsg. Therapeutic Endoscopy and Radiology of the Gut. 2. Aufl. Hodder Arnold, 1998.

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Buchteile zum Thema "Fibre digestion"

Gidenne, T., R. Caraba�o, R. Abad-Guam�n, J. Garc�a und C. de Blas. „Fibre digestion.“ In Nutrition of the rabbit, 69–88. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789241273.0069.

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Flourié, B. „The Influence of Dietary Fibre on Carbohydrate Digestion and Absorption“. In Dietary Fibre — A Component of Food, 181–96. London: Springer London, 1992. http://dx.doi.org/10.1007/978-1-4471-1928-9_10.

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Eggum, B. O. „The Influence of Dietary Fibre on Protein Digestion and Utilisation“. In Dietary Fibre — A Component of Food, 153–65. London: Springer London, 1992. http://dx.doi.org/10.1007/978-1-4471-1928-9_8.

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Johnson, I. T. „The Influence of Dietary Fibre on Lipid Digestion and Absorption“. In Dietary Fibre — A Component of Food, 167–80. London: Springer London, 1992. http://dx.doi.org/10.1007/978-1-4471-1928-9_9.

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Bautil, A., J. Verspreet, J. Buyse, P. Goos, M. R. Bedford und C. M. Courtin. „Chapter 12 Adaptation of the microbiome towards fibre digestion: effects of age and dietary ingredients“. In The value of fibre, 199–216. The Netherlands: Wageningen Academic Publishers, 2019. http://dx.doi.org/10.3920/978-90-8686-893-3_12.

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Montoya, Carlos A., Suzanne Hodgkinson und Paul J. Moughan. „Tools and Methods to Quantify the Digestion of Protein, Lipid, Starch and Fibre from a Chemistry/Microbiology Perspective“. In Interdisciplinary Approaches to Food Digestion, 199–229. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-03901-1_10.

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Moore, Kenneth J. Moore, und Dwayne R. Buxton. „Fiber Composition and Digestion of Warm-Season Grasses“. In Native Warm-Season Grasses: Research Trends and Issues, 23–33. Madison, WI, USA: Crop Science Society of America and American Society of Agronomy, 2015. http://dx.doi.org/10.2135/cssaspecpub30.c2.

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Venema, Koen. „Impact of Fiber on Gastrointestinal Microbiota“. In Nondigestible Carbohydrates and Digestive Health, 125–64. Oxford, UK: Wiley-Blackwell, 2011. http://dx.doi.org/10.1002/9780470958186.ch6.

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Rose, Devin J., und Bruce R. Hamaker. „Overview of Dietary Fiber and its Influence on Gastrointestinal Health“. In Nondigestible Carbohydrates and Digestive Health, 185–221. Oxford, UK: Wiley-Blackwell, 2011. http://dx.doi.org/10.1002/9780470958186.ch8.

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Paeschke, Teri M., und William R. Aimutis. „Introduction to Fiber and Nondigestible Carbohydrates: Definition, Health Aspects, and Perspectives“. In Nondigestible Carbohydrates and Digestive Health, 1–13. Oxford, UK: Wiley-Blackwell, 2011. http://dx.doi.org/10.1002/9780470958186.ch1.

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Konferenzberichte zum Thema "Fibre digestion"

Asmatulu, R., und V. Movva. „Evaluation of Advanced Composites Using Destructive Testing Techniques“. In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-62754.

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A very basic problem in manufacturing advanced composites is the occurrence of defects, such as voids, delamination, resin rich areas, distorted fibers and foreign material inclusions. Of these defects, voids are the unavoidable defects mostly coming from a variety of reasons, including resin type, poor vacuum source, air entrapment, leakage of vacuum bag and by-products formed during the curing cycle of laminates. These voids seriously affect the mechanical properties of composite panels, and cause severe damages during the service. In the present study, various destructive testing techniques, such as visual, burn-out or ignition loss of cured composites, image analysis and matrix digestion or acid digestive were used on advanced composite panels at various porosity levels (e.g., 0–2%, 3–6% and greater than 7%), and the obtained results were compared each other. Also, testing parameters were optimized using the standard samples to achieve maximum possible error free data of voids, fiber volume, fiber wash, inter laminar and trans laminar cracks, miss-oriented fibers, lay-up orders and latent defects. Optimization of parameters was performed using the design of experiments. We determined that the test results were significantly changed based on the test techniques, composite samples, as well as testing time, operator skill, cost of experimentation, initial equipment cost, pre and post treatments and lack of standardized testing patterns.

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Keleman, Michael P. „Economics of Wastewater Treatment Codigestion“. In ASME 2010 4th International Conference on Energy Sustainability. ASMEDC, 2010. http://dx.doi.org/10.1115/es2010-90397.

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Wastewater treatment is the method by which sewage of both residential and industrial sources is processed to promote public health and reduce environmental impacts on receiving waters. This physical and biological process generates sludge, which after being treated to reduce pathogens, is referred to as biosolids. In the US there are over 16,000 wastewater treatment plants (WWTP), and every year they produce approximately 7 million tons of biosolids according to the EPA.1 These biosolids are handled differently depending upon local conditions, but most are either buried in landfills, land applied for agriculture or incinerated. Reducing the volume of biosolids produced by each facility is desirable for improving operational efficiency since lower volumes are easier to manage and cheaper to handle and dispose. Most facilities utilize either aerobic digestion to process sludge into biosolids, but larger facilities use anaerobic digestion because this process reduces the overall volume of solids left for management. Anaerobic digestion is more complex and capital intensive, so typically only those facilities treating flows higher than 5 million gallons per day (MGD) use anaerobic digestion. Given current economic conditions and rising energy costs, however, anaerobic digestion is becoming more attractive to utility managers as they attempt to offset energy costs. The anaerobic process produces methane gas. Also called biogas, methane can be utilized not only to fire boilers for heating digesters and nearby buildings, but also to fuel internal combustion engines, microturbines or fuel cells to generate power for plant processes such as blowers in the aeration system. There is also the potential for WWTPs to obtain carbon credits for utilizing renewable energy, especially in those states with renewable portfolio standards. Because anaerobic digestion has limited application in the US, this study evaluated economic viability at plants with design flows less than 5 MGD by incorporating codigestion of food waste to improve the production of biogas for use as energy to reduce operational costs and recover capital costs.

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Takasaki, Rikiya, Lee Chang Yuan, Hirotsugu Kamahara, Youichi Atsuta und Hiroyuki Daimon. „Effect of acid detergent fiber in hydrothermally pretreated sewage sludge on anaerobic digestion process“. In PROCEEDINGS OF THE INTERNATIONAL CONFERENCE OF GLOBAL NETWORK FOR INNOVATIVE TECHNOLOGY AND AWAM INTERNATIONAL CONFERENCE IN CIVIL ENGINEERING (IGNITE-AICCE’17): Sustainable Technology And Practice For Infrastructure and Community Resilience. Author(s), 2017. http://dx.doi.org/10.1063/1.5005697.

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San, Hongli. „The Development of Fluorescence Optical Fiber Temperature Measurement System Based on Microwave Digestion Instrument“. In 2011 International Conference on Computational and Information Sciences (ICCIS). IEEE, 2011. http://dx.doi.org/10.1109/iccis.2011.288.

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Araújo, Ascanio D., Arnab Majumdar, Harikrishnan Parameswaran und Béla Suki. „Dynamics Of Enzyme Digestion Of A Single Elastic Fiber Under Tension: An Anisotropic Diffusion Model“. In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a2893.

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Amin, S., und B. Suki. „Modeling Elastin Fiber Digestion as a Set of Self-Interacting Molecules Capable of Unfolding Binding Sites During Stretch“. In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a2257.

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Hasain, Zubaidah, Nor Azmi Kamaruddin, Nor Azlin Mohamed Ismail, Tong Seng Fah, Nurul Huda Razalli, Norfilza Mohd Mokhtar und Raja Affendi Raja Ali. „IDDF2019-ABS-0172 Gut microbiota shift and low fibre intake in post gestational diabetes women“. In International Digestive Disease Forum (IDDF) 2019, Hong Kong, 8–9 June 2019. BMJ Publishing Group Ltd and British Society of Gastroenterology, 2019. http://dx.doi.org/10.1136/gutjnl-2019-iddfabstracts.180.

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Ou, Junjie, Shuwen Wang, Carolyn L. Ren und Janusz Pawliszyn. „Preparation of Poly(Dimethylsiloxane) Chip-Based Cartridge for Isoelectric Focusing and Whole-Channel Imaging Detection“. In 2008 Second International Conference on Integration and Commercialization of Micro and Nanosystems. ASMEDC, 2008. http://dx.doi.org/10.1115/micronano2008-70303.

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A poly(dimethylsiloxane) (PDMS) microfluidic chip-based cartridge was fabricated by sandwiching commercial dialysis membrane and inserting fused-silica capillary into the end of channel according to the principle and structure of a commercial fused-silicon capillary-based cartridge, which can adapt to an IEF analyzer for isoelectric focusing with whole channel imaging detection (IEF-WCID). The novel design of sandwiching membrane in this chip not only eliminated the unfavorable hydrodynamic pressure, leading to poor IEF reproducibility, but made the sample injection much easy. Thus the reproducibility of analysis was very good. The prepared microfluidic chips were applied for qualitative and quantitative analysis of proteins. The six pI markers in the range of 3–10 were separated by IEF under the optimized conditions. The pH gradients exhibited good linear by plotting the pI versus peak position, and the correlation coefficient reached to 0.9994 and 0.9995. The separation of more complicated human hemoglobin control and myoglobin sample could be achieved. By comparison with the separation efficiency obtained on the microfluidic chip and commercial cartridge, the results were similar, which indicates the capillary cartridge may be replaced with the cost-efficient PDMS microfluidic chip. It is anticipated the high throughput analysis can be easily performed on this microfluidic chip patterned multi-channels. The techniques of capillary electrophoresis (CE) have been extensively explored for the chip-based separation. Isoelectric focusing (IEF) as one of high-resolution CE techniques has been widely applied for the separation of zwitterionic biomolecules, such as proteins and peptides. After the samples were focused at their corresponding pIs, the focused zones were mobilized to pass through the detection point for obtaining an electropherogram. This single-point detection imposes extensive restriction for chip-based IEF because a mobilization process requires additional time and lowers resolution and reproducibility of the separation [1]. An alternative is whole-column imaging detection developed by Pawliszyn et al [2] is an ideal detection method for IEF because no mobilization is required, which avoids the disadvantages as mentioned previously. Most microfluidic systems could be fabricated in glass/silicon or polymers in which the channels are defined using photolithography and micromachining. Mao and Pawliszyn [3] have developed a method for IEF on an etched quartz chip following whole-channel imaging detection (WCID). Ren et al [4] presented an integrated WCID system on glass microfluidic chip. However, these materials have some disadvantages such as expensive and fragile and so on. An attractive alternative for fabrication of microfluidic devices is using poly(dimethylsiloxane) (PDMS) as material, which has unique properties such as nontoxic, optical transparent down to 280 nm, elastomeric, hydrophobic surface chemistry Yao et al. [5] designed the glass/PDMS microchip integrated whole-column fluorescence imaging detection for IEF of R-phycoerythrin. Our preliminary studies have successfully developed a PDMS chip-based cartridge for IEF-WCID. It is due to hydrodynamic flow between two reservoirs that the focused zones were mobilized, thus gave poor reproducibility and difficulty in sample infusion. As membranes have been integrated into microchips for microdialysis, protein digestion, solid-phase extraction, desalting, pumping and so on, it could minimize hydrodynamic flow by using membranes as a filter. Although a simple PDMS chip-based cartridge has been successfully fabricated in our labs according to the principle of commercial capillary-based cartridge, it is difficult to introduce the sample into channel for IEF-WCID. As the vacuum was applied in one end of channel for infusing of solution into channel, the lifetime of this chip-based cartridge is shortened. Additionally, the hydrodynamic flow is occurred due to the different heights of anolyte and catholyte in two reservoirs, respectively. The IEF separation was deteriorated by the infusion of anolyte or catholyte, thus leading to poor reproducibility of IEF-WCID analysis. Similar to the hollow fiber in the commercial capillary-based cartridge in which it is aimed to separate the sample in the capillary and electrolytes in the reservoirs, porous membrane was integrated into PDMS chips for decrease of hydrodynamic flow [6]. As a result, integration of dialysis membrane is considered into the design of our new chip-based cartridge. Up to now, many approaches have been described to integrate membranes into glass/quartz or polymeric microfluidic chips. A simple method is direct incorporation by gluing or clamping commercial flat membranes. A major problem of this method is sealing, otherwise, a phenomenon of leakage around the membranes is always occurred due to the capillary force. A novel approach of sandwiching dialysis membrane was developed as schematically indicated in Figure 1. After optimizing IEF conditions, the separation of pI markers was performed on the obtained PDMS microfluidic chip. As exhibited in Figure 2a, six pI markers could be well separated on the PDMS chips patterned the channel of 100 μm deep, 100 μm wide by IEF-WCID. All the peaks were sharp and symmetric, indicating that both EOF and analytes adsorption were completely suppressed by the dynamic coating of PVP. The plots of peak position versus pI of these pI markers suggested good linearity of pH gradient (as shown in Figure 2b). The linear correlation coefficient was 0.9995 (n = 6). As expected to the capillary-based cartridge, the PDMS microfluidic chips could be applied for qualitative and quantitative analysis of proteins. Figure 3a exhibited that human hemoglobin control AFSC contains four known isoforms (HbA, HbF, HbS and HbC) mixed with two pI marker 6.14 and 8.18 were well separated on the PDMS chip by IEF-WCID, indicating the strong separation ability of chip similar to the commercial capillary-based cartridge. According to the linearity of pH gradient, these four isoforms with the pIs of 7.0, 7.1, 7.3 and 7.5, respectively, could be detected. An unknown isoform in human hemoglobin control marked asterisk in Figure 3A observed besides the definite four isoforms A, F, S and C. The myoglobin from horse heart contains two isoforms, whose pIs are 6.8 and 7.2, respectively. It can be seen from Figure 3b that these two isoforms were separated on PDMS chip by IEF-WCID. The peak 1 and 2 could be assigned to the two isoforms according to their pI. The pI of unknown peak marked asterisk could be measured to 6.25.

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Berichte der Organisationen zum Thema "Fibre digestion"

Pemberton, R. G., D. Edser und MRL Gower. Optimisation of acid digestion conditions for volume fraction measurements of hard to digest fibre-reinforced polymer composites. National Physical Laboratory, September 2020. http://dx.doi.org/10.47120/npl.mn12.

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Rapid Integrated Method for Total Dietary Fiber. Cereal & Grains Association, 2021. http://dx.doi.org/10.1094/aaccintmethod-32-60.01.

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This method determines total dietary fiber (TDF) in foods and food ingredients, as defined by Codex Alimentarius. The method measures soluble and insoluble dietary fiber, including resistant starch, as well as nondigestible oligosaccharides. In this method, enzymatic digestion is used to simulate human intestinal digestion. Insoluble dietary fiber (IDF) and soluble dietary fiber that precipitates in 78% ethanol (SDFP) are separated by filtration and quantified gravimetrically. Additionally, highly soluble oligosaccharides (SDFS) are quantified by chromatographic separation. TDF is reported as the sum of the gravimetric and high-performance liquid chromatography (HPLC) results. The digestion and chromatographic conditions of this method have been modified from those of AACC Approved Methods 32-45.01 and 32-50.01 in an attempt to better simulate human digestion and to allow for more exact quantitation.

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