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Auswahl der wissenschaftlichen Literatur zum Thema „Faecal contamination“
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Zeitschriftenartikel zum Thema "Faecal contamination"
1
Amoroso, Caroline R., Alexa G. Frink, and Charles L. Nunn. "Water choice as a counterstrategy to faecally transmitted disease: an experimental study in captive lemurs." Behaviour 154, no. 13-15 (2017): 1239–58. http://dx.doi.org/10.1163/1568539x-00003466.
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Abstract Many parasites and pathogens are transmitted via water, including through faecal contamination of water sources. Yet water is essential for survival, and some species gain nutritional and other benefits from coprophagy. We investigated how primates balance the risks of faecal pathogen transmission with potential benefits of faeces ingestion in their selection of water sources by conducting behavioural experiments with five species of lemurs (Family Lemuridae) in captivity. Subjects were given a choice between clean water and water ‘contaminated’ with disinfected faecal material, which contained cues associated with faecally transmitted parasites, but minimal risk. We found that lemurs exhibited strong preferences for the clean water. This pattern was supported even at low levels of faecal contamination and in species adapted to water-limited habitats, for which choosiness about water quality could present a dehydration risk. Our results strongly support the hypothesis that avoiding faecal contamination is important in water selection.
2
Baudišová, D. "Microbial pollution of water from agriculture." Plant, Soil and Environment 55, No. 10 (October 21, 2009): 429–35. http://dx.doi.org/10.17221/131/2009-pse.
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Microbial contamination of small streams in agricultural areas was monitored for two years. Microbiological indicators of faecal pollution (faecal coliforms, <I>Escherichia coli</I> and intestinal enterococci were detected by standard methods based on the cultivation of bacteria on selective media). The obtained results showed that running contamination of streams from agricultural areas was not extremely high, but it showed marked seasonal fluctuations (the average values and maximal values revealed great differences). Microbial contamination also increased several times in relation to high precipitation. The water quality in three (and/or four) localities exceeded the acceptable counts of faecal coliforms and enterococci given by the Czech legislation (40 CFU/ml for faecal coliforms and 20 CFU/ ml for enterococci). In agriculturally polluted streams, there were detected more enterococci than faecal coliforms, and also some less frequent species related to farm animals (<I>Streptococcus equines</I> and <I>S. bovis</I>) or plant rests (<I>E. mundtii, E. gallinarum, E. casseliflavus</I>) were present. <I>E. faecalis</I> and <I>E. faecium</I> strains (these are the most common species related to human faecal pollution) were less frequent there.
3
Leeming, Rhys, Jonathan S. Stark, and James J. Smith. "Novel use of faecal sterols to assess human faecal contamination in Antarctica: a likelihood assessment matrix for environmental monitoring." Antarctic Science 27, no. 1 (June 25, 2014): 31–43. http://dx.doi.org/10.1017/s0954102014000273.
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AbstractWastewater containing human sewage is often discharged with little or no treatment into the Antarctic marine environment. Faecal sterols (primarily coprostanol) in sediments have been used for assessment of human sewage contamination in this environment, butin situproduction and indigenous faunal inputs can confound such determinations. Using gas chromatography with mass spectral detection profiles of both C27and C29sterols, potential sources of faecal sterols were examined in nearshore marine sediments, encompassing sites proximal and distal to the wastewater outfall at Davis Station. Faeces from indigenous seals and penguins were also examined. Faeces from several indigenous species contained significant quantities of coprostanol but not 24-ethylcoprostanol, which is present in human faeces.In situcoprostanol and 24-ethylcoprostanol production was identified by co-production of their respectiveepi-isomers at sites remote from the wastewater source and in high total organic matter sediments. A C29sterols-based polyphasic likelihood assessment matrix for human sewage contamination is presented, which distinguishes human from local fauna faecal inputs andin situproduction in the Antarctic environment. Sewage contamination was detected up to 1.5 km from Davis Station.
4
Awuah, Frank. "Application of microorganisms to determine the impact of infiltration layer and season on pit latrine groundwater contamination." Sustinere: Journal of Environment and Sustainability 8, no. 3 (December 30, 2024): 357–67. https://doi.org/10.22515/sustinere.jes.v8i3.425.
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This study was necessitated as a result of the frequent cases of diarrhoea observed among the students of the researcher. The researcher employed faecal indicator bacteria to assess the impact of the infiltration layer and seasonal variations on groundwater contamination from pit latrines in the municipalities where the students reside. The main experimental materials consisted of water samples collected from 15 randomly selected wells in the Tano Districts of Ghana. Total coliforms, faecal coliforms, and enterococci were used as faecal indicators. The Most Probable Number (MPN) method was employed to determine the presence of faecal indicators in the water samples. The results showed that enterococci and faecal coliforms were reliable indicators of human faecal contamination than total coliforms. The study revealed that coliform level (indicating pit latrine groundwater contamination) increased with greater pit depth and lower static water levels. Based on these findings, it is recommended that the future studies on human faecal contamination prioritize enterococci and faecal coliforms over total coliforms as indicators. To mitigate groundwater contamination from pit latrines, the equation EC = 0.12(PLD) – 0.09(SWL) + 2.37 can be applied to predict a safer infiltration layer between the bottom of pit latrines and the water table.
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Leeming, R., N. Bate, R. Hewlett, and P. D. Nichols. "Discriminating faecal pollution: a case study of stormwater entering Port Phillip Bay, Australia." Water Science and Technology 38, no. 10 (November 1, 1998): 15–22. http://dx.doi.org/10.2166/wst.1998.0369.
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This investigation was designed to provide preliminary information to the Environment Protection Authority concerning the input of faecal matter to stormwater drains in the Rippleside area of Geelong, Victoria. Results derived from the combined use of sterol biomarkers (e.g. coprostanol and 24-ethylcoprostanol) and four sub-groups of bacterial indicators (e.g. thermotolerant coliforms, E. coli, faecal streptococci and enterococci) indicated that during wet weather, all sites sampled were affected by significant human faecal contamination. Ratios of coprostanol to bacterial indicators were similar to those for samples collected from nearby sewer mains. During dry weather, there were still severely elevated levels of faecal contamination based on bacterial indicators, but correspondingly low concentrations of faecal sterols suggesting minimal human or herbivore faecal contamination. The origin of the majority of the faecal pollution in dry weather therefore remains to be fully explained. It is clear from this and related studies that the combined measurement of faecal sterols and bacterial indicators can greatly assist distinguishing sources of faecal pollution. It is also shown for aquatic environments that the measurement of coprostanol or other single indicators alone, is inadequate to fully discern faecal contamination from human sources.
6
Godfree, A. F., D. Kay, and M. D. Wyer. "Faecal streptococci as indicators of faecal contamination in water." Journal of Applied Microbiology 83, S1 (October 1997): 110–19. http://dx.doi.org/10.1046/j.1365-2672.83.s1.12.x.
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Wicki, Melanie, Adrian Auckenthaler, Richard Felleisen, Marcel Tanner, and Andreas Baumgartner. "Novel Bacteroides host strains for detection of human- and animal-specific bacteriophages in water." Journal of Water and Health 9, no. 1 (February 3, 2011): 159–68. http://dx.doi.org/10.2166/wh.2010.165.
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Bacteriophages active against specific Bacteroides host strains were shown to be suitable for detection of human faecal pollution. However, the practical application of this finding is limited because some specific host strains were restricted to certain geographic regions. In this study, novel Bacteroides host strains were isolated that discriminate human and animal faecal pollution in Switzerland. Two strains specific for bacteriophages present in human faecal contamination and three strains specific for bacteriophages indicating animal faecal contamination were evaluated. Bacteriophages infecting human strains were exclusively found in human wastewater, whereas animal strains detected bacteriophages only in animal waste. The newly isolated host strains could be used to determine the source of surface and spring water faecal contamination in field situations. Applying the newly isolated host Bacteroides thetaiotaomicron ARABA 84 for detection of bacteriophages allowed the detection of human faecal contamination in spring water.
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Mudau, Mulalo, Renay Ngobeni-Nyambi, and Maggy Ndombo Benteke Momba. "The Identification of Predominant Faecal Contamination Sources in Water Using Host-Specific Genetic Markers in Water-Stressed Rural Communities of Vhembe District Municipality, South Africa." Water 16, no. 23 (December 3, 2024): 3477. https://doi.org/10.3390/w16233477.
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It is critical to attribute faecal contamination to its original source in order to assess public health risks and implement effective interventions to mitigate future contamination. This study aimed to identify the primary sources of faecal contamination in water using microbial source tracking markers in water-stressed rural communities. A total of 1128 water samples were collected sequentially from the main source (river/borehole) to the households. Six host-specific genetic markers were used to detect faecal contamination in the water samples (BacHum and HF183, BacCow, Pig-2-Bac, Cytb and BacCan). Of the 564 water samples tested during the wet season, 37.94% (n = 214) were positive for human and animal-specific Bacteroidales marker genes, while 31.73% (n = 179) of the 564 tested during the dry season were also positive. During the wet season, animal faecal contamination was more prevalent among the positive samples (Cytb: 20.56%, n = 44; BacCan: 19.16%, n = 41). By contrast, human-origin faecal contamination was dominant during the dry season (BacHum: 23.46%, n = 42; HF183: 21.23%, n = 38). Identifying the origin of faecal contamination will assist in implementing targeted intervention strategies for the effective prevention of pathogen transmission in water-stressed rural communities in order to protect public health.
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van Lieverloo, J. Hein M., E. J. Mirjam Blokker, and Gertjan Medema. "Quantitative microbial risk assessment of distributed drinking water using faecal indicator incidence and concentrations." Journal of Water and Health 5, S1 (September 1, 2007): 131–49. http://dx.doi.org/10.2166/wh.2007.134.
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Quantitative Microbial Risk Assessments (QMRA) have focused on drinking water system components upstream of distribution to customers, for nominal and event conditions. Yet some 15—33% of waterborne outbreaks are reported to be caused by contamination events in distribution systems. In the majority of these cases and probably in all non-outbreak contamination events, no pathogen concentration data was available. Faecal contamination events are usually detected or confirmed by the presence of E.coli or other faecal indicators, although the absence of this indicator is no guarantee of the absence of faecal pathogens. In this paper, the incidence and concentrations of various coliforms and sources of faecal contamination were used to estimate the possible concentrations of faecal pathogens and consequently the infection risks to consumers in event-affected areas. The results indicate that the infection risks may be very high, especially from Campylobacter and enteroviruses, but also that the uncertainties are very high. The high variability of pathogen to thermotolerant coliform ratios estimated in environmental samples severely limits the applicability of the approach described. Importantly, the highest ratios of enteroviruses to thermotolerant coliform were suggested from soil and shallow groundwaters, the most likely sources of faecal contamination that are detected in distribution systems. Epidemiological evaluations of non-outbreak faecal contamination of drinking water distribution systems and thorough tracking and characterisation of the contamination sources are necessary to assess the actual risks of these events.
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Höglund, C., T. A. Stenström, H. Jönsson, and A. Sundin. "Evaluation of faecal contamination and microbial die-off in urine separating sewage systems." Water Science and Technology 38, no. 6 (September 1, 1998): 17–25. http://dx.doi.org/10.2166/wst.1998.0232.
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An important feature for recirculated products from a sewage system is that the risk for transmission of disease is low. In a urine source separating system faeces may contaminate the collected urine through the toilet. The concentrations of faecal indicator organisms were measured in 14 urine collection tanks. The survival of these and some pathogenic microorganisms in source separated human urine was studied. Total coliforms, E. coli and clostridia were found in low concentrations while faecal streptococci occurred in concentrations up to 105/ml urine solution. The results from phenotyping of faecal streptococci and the high counts indicate growth within the system. The majority of the pathogenic microorganisms investigated had a rapid die-off. A higher temperature, a low degree of dilution and a pH far from neutral had a negative effect on survival. E. coli had a rapid die-off and is not suitable for indicating faecal contamination of the collected urine. Quantification of faecal sterols is a possible alternative to microbial analysis for this purpose. The amounts of coprostanol showed that the faecal contamination varied from not detected to up to 13.3 mg/ml urine solution with a concentration in the urine sludge of up to 417.5 mg/kg.
Dissertationen zum Thema "Faecal contamination"
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Turner, Susan Jane. "Microbiological Indicators of Faecal Contamination in Aquatic Environments." Thesis, University of Auckland, 1996. http://hdl.handle.net/2292/2375.
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1. The aim of this research was to determine appropriate microbial indicator systems for use in monitoring the occurrence and source of faecal contamination in aquatic environments. Particular emphasis has been placed on the identification of indicators which might enable faecal contamination of human origin to be distinguished from that of non-human derivation. Two lines of investigation have been pursued; the use of alternative microbial indicator systems for water quality monitoring, and the development of diagnostic molecular methods for identifying the origin of faecal organisms. 2. Field and treatment plant studies were undertaken to investigate the utility of three indicator systems for monitoring water quality impacts in the coastal area surrounding the discharge from Auckland's North Shore City wastewater treatment plant. F-specific bacteriophage (F-phage) and ratios of F-phage to enterococci and faecal coliforms were examined over a twelve month period in which indicator levels at various stages of the wastewater treatment process were measured. These indicators and levels of Clostridium perfringens spores in sediment were measured in four outfall impact studies. 3. F-phage levels in effluent from the treatment plant (median=2.5 x 102 cfu/l00 ml) were too low to enable their use as source-specific tracers of effluent dispersion. However, higher F-phage levels in raw sewage (median=6.2 x l05 cfu/l00 ml) suggested that phage may be an appropriate indicator of raw sewage contamination in the receiving environment. F-phage was detected in coastal waters remote from the sewage outfall, providing presumptive evidence of sewage contamination entering the sea from sources other than the outfall discharge itself. 4. F-phage/enterococci and F-phage/faecal coliform ratios that were determined for sewage and treated effluent derived from the North Shore plant proved to be highly variable and were therefore unreliable for determining the source faecal contamination. 5. The concentration of Clostridium perfringens spores in sediments did not provide a useful means for tracing the distribution of faecal contaminants in the coastal system because the spore levels were found to be similar throughout the study area. This widespread occurrence of C. perfringens spores prevented identification of defined impact zones for any of the potential sources of faecal contaminants examined. 6. Randomly Amplified Polymorphic DNA (RAPD) analysis was used to investigate whether genetic markers could be identified for Escherichia coli isolates derived from different sources. RAPD profiling was found to be a highly discriminative method able to subtype E. coli strains present in both sewage and in the wastewater stream of a mechanical wood-pulping operation. In the pulp mill effluent, RAPD analysis indicated the presence of a restricted range of E. coli strains compared to the range found in the water intake and sewage effluent discharges. The results obtained provide presumptive evidence for the multiplication of E. coli strains within the wastewater stream. 7. Comparison of RAPD profiles derived from a range of human and environmental E. coli isolates led to the identification of a 1.6-kb marker. present in a subset of human isolates, but found only infrequently in isolates from animal origin. Primers based on sequence information obtained from this region of the genome yielded a direct PCR assay for the 'human' marker. The reliability and utility of the assay was improved when fluorescently-labelled primers and an automated DNA detection system was employed for the analysis of the amplified PCR products. 8. The diagnostic polymorphism was localised to the region immediately downstream of the gene encoding the glycine decarboxylase P-protein (gcvP). Comparison of DNA sequences from this region in 'marker-positive' and 'marker-negative' E. coli isolates revealed an abrupt loss of homology immediately downstream of the transcription termination point of the gcvP gene. The region of non-homology extends for at least 130 base pairs beyond the gcvP transcription terminator. Sequences spanning this region in marker-negative isolates exhibited a high level homology with that determined for E. coli K-12. The origin of the sequence for the same region in marker-positive isolates was unable to be identified. 9. The utility of the marker for environmental monitoring was investigated in a field study of a rural stream receiving bacterial contamination of both human and animal origin. The marker was consistently detected both in isolates derived from sewage effluents and in those found immediately downstream from sewage inputs-despite the presence of a high background of E. coli of animal origin. However the utility of the marker as a sewage-specific tracer was limited by the relatively low numbers of marker-positive d. coli in sewage effluents (approximately 8%). 10. In summary, the work described in this thesis has confirmed the need for multiple indicator systems in water quality monitoring and has identified appropriate areas for further research. The most promising area for future research is in the development of molecular tools which might provide both a better understanding of genetic variation in indicator organisms and enable methods to be developed for the detection of host-specific strains. Location of other polymorphisms likely to be present in the E. coli genome should enable further host-specific markers to be identified which could complement the 'human' marker identified in this study.<br>Whole document restricted, but available by request, use the feedback form to request access.
2
Miller, Layla. "Determining specific sources of faecal contamination within shellfisheries." Thesis, University of Exeter, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.495762.
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Geary, Phillip M., University of Western Sydney, of Science Technology and Environment College, and of Science Food and Horticulture School. "On-site system effluent source tracking using geochemical and microbial tracers in a coastal catchment." THESIS_CSTE_SFH_Geary_P.xml, 2004. http://handle.uws.edu.au:8081/1959.7/566.
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The principal aims of this thesis were to examine whether there were hydraulic links between individual on-site wastewater systems in sandy soils at Salt Ash and the Tilgerry Creek estuary near Port Stephens, New South Wales, and whether the chemical and microbiological contaminants from on-site systems could reach surface and groundwaters, and possibly lead to impacts on estuarine oyster growing waters. The research outcomes are contained within the thesis and in four refereed papers presented at conferences, and which have been subsequently published, or are in press. Copies of each of these papers are contained within the thesis Appendices. The presence of faecal contamination from domestic systems in the estuary, and surface drains in particular, has been confirmed by work contained in this thesis. The potential level of risk to human health from the consumption of contaminated oysters is, however, regarded as very low, although an assessment of health risk using established microbial assessment models has not been undertaken<br>Doctor of Philosophy (PhD)
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Leeming, Rhys, and n/a. "Coprostanol and related sterols as tracers for feacal contamination in Australian aquatic environments." University of Canberra. School of Resource, Environmental & Heritage Sciences, 1996. http://erl.canberra.edu.au./public/adt-AUC20060816.172519.
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Pollution from human and animal faecal waste is a major cause of deteriorating water
quality and increased nutrient loads in coastal and inland waterways. Management of
this problem depends on knowing which sources of faecal matter are the cause and
what is the degree and extent of the pollution. Bacterial indicator organisms have long
been the principal method used to test water samples for faecal contamination.
However, none of the currently used bacterial indicators on their own are source
specific enough to distinguish different sources of faecal matter. The use of faecal
sterol biomarkers in conjunction with existing bacterial indicators offers a new way to
distinguish sources of faecal contamination.
This study investigates the sources of faecal sterols, the relationship of coprostanol to
existing bacterial indicators of faecal pollution, the degradation of faecal sterols and the
problem of determining the sources of faecal contamination and the distribution of
faecal contamination using faecal sterol biomarkers. 5p-Stanols (i.e. faecal sterols)
were found to be significant constituents of human, herbivore (i.e. cows, sheep etc.)
and pig and cat faeces. Human faeces contained 73 ± 4% coprostanol in relation to
the sum of coprostanol and 24-ethylcoprostanol and primary treated effluent contained
86 ± 0.4% coprostanol. Herbivore faeces contained 38 ± 4% coprostanol and 62 ±
4% 24-ethylcoprostanol whereas pig faeces contained 50 � 5% of each compound.
Both birds and dogs faeces contained either trace amounts of 5B-stanols or they could
not be detected. Notable differences were observed in the abundance of Closthdium
perfringens spores between the faeces of birds and domestic pets such as cats and
dogs. The above differences were subsequently exploited to distinguish faecal
contamination in Lake Tuggerah. An examination of the relationships between
coprostanol and bacterial indicator concentrations from several environments revealed
that 60 and 400 ng L of coprostanol corresponded to currently defined primary and
secondary contact limits for bacteria measured as either thermotolerant coliforms or
enterococci in the environment.
Four degradation experiments showed faecal sterols and related sterols such as
cholesterol decay at similar rates. An induction period was observed in all
experiments which meant that simple exponential equations to describe the rate of
decay of coprostanol were inadequate; a complimentary log - log transformation of the
data was used and the equation:
Y = l-Exp(-Exp(time x -0.01 + temp x -0.158 + 3.33)) x 100
was derived where Y equals the predicted percentage of coprostanol remaining over
time at a given temperature. In terms of persistence in the environment, Clostridium
perfringens spores > coprostanol > enterococci > thermotolerant coliforms.
Two field studies were undertaken to highlight the use of faecal sterols. In the Lake
Tuggerah study, the results indicated that faecal contamination of receiving waters in
the Tuggerah Lakes during rain events was significant, but was not derived from
human faecal matter; rather it appears to be principally derived from native birds and,
to a lesser extent, domestic pets. In the Derwent Estuary study, based on the
distribution of the faecal biomarker coprostanol, the mid estuary and parts of the upper
estuary (from Newtown Bay to Taroona), were found to be severely contaminated by
sewage. In summary, the use of faecal sterols to trace faecal contamination were found
to be an invaluable addition to the tools water managers use to investigate faecal
pollution.
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Florini, Styliani. "Identification of species-specific source(s) of faecal contamination in Salcott Creek, Blackwater Estuary." Thesis, University of Essex, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416715.
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Lang, Cassandra C., and University of Lethbridge Faculty of Arts and Science. "Identification and metabolic characterization of host-specific enterococci for use in source-tracking faecal contamination." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2005, 2005. http://hdl.handle.net/10133/265.
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Metabolic were used to evaluate Enterococcus as an indicator of faecal pollution. Enterococci were isolated using m-Enterococcus agar and speciated using conventional biochemical tests. Forty percent of the isolates were identified and metabolically characterized by the automated Biolog system. The biochemical test scheme recognized 16 enterococcal species, while Biolog recognized nine. Both methods identified E. faecalis at the greatest frequency. Overall species frequencies varied between the two methods. Biolog was unable to identify 31% of the isolates; 7% of the isolates were unidentified by the biochemical test scheme. Of the identified isolates, metabolic profiling with Biolog achieved speciation with 60 substrates. Unique profiles were obtained for 89% of the isolates. Isolates also demonstrated inter-trial differntial metabolism of substrates. This and the large number of unidentified isolates suggest great diversity among enterococci. Diversity and inter-trial metabolic inconsistencies will complicate use of enterococcal metabolic profiles as a source-tracking tool.<br>xxiii, 264 leaves ; 29 cm.
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Benselfelt, Tobias. "Flow Cytometry Sensor System Targeting Escherichia Coli as an Indicator of Faecal Contamination of Water Sources." Thesis, Linköpings universitet, Teknisk biologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-108004.
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Poor water quality is a global health concern affecting one billion people around the world. It is important to monitor water sources in order to maintain the quality of our drinking water and to avoid disease outbreaks. Targeting Escherichia coli as a faecal indicator is a widely used procedure, but the current methods are time consuming and not adequate to prevent spreading of faecal influence. This Master thesis demonstrates the development of a near infrared fluorescence flow cytometer sensor system targeting Escherichia coli, using fluorescently labeled chicken IgY antibodies. The near infrared light was chosen to avoid fluorescence from blue-green algae that are present in the water source. The hardware was developed with a 785 nm laser line to detect Alexa Fluor 790 labeled antibodies, using a photomultiplier tube or two different CMOS cameras. The antibodies were labeled using a commercial labeling kit, and evaluated using antibody binding assays and the developed hardware. The IgY antibodies were successfully labeled with Alexa Fluor 790 and the function was maintained after the labeling process. The result demonstrates the principles of the sensor system and how it solved to the problem with fluorescence from blue-green algae. An aperture was used to overcome the suboptimal laser and filter setup, and to increase the sensitivity of the system. However, only a small fraction of the cells could be detected, due to challenges with the focal depth and loss of sensitivity in the photomultiplier tube at near infrared wavelengths. Further development is required to create a working product.
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Luyt, Catherine Diane. "Faecal source tracking and water quality in the Eastern Cape, South Africa." Thesis, Rhodes University, 2013. http://hdl.handle.net/10962/d1018242.
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Water quality is concerning as many still lack access to safe drinking water. Alternate sources such as rivers (FC up to 1600 CFUs/100 mL) and rainwater are often polluted. Rainwater tanks require maintenance to improve water quality, but could be used for non-potable purposes or irrigation. Grahamstown infrastructural failures initiate deviations from DWAF 1996 domestic water guidelines for microorganisms within the distribution system. Frequent testing can decrease risks of waterborne diseases. Limitations to this are inaccessibility of rural areas, distances from testing centres and costs. The low cost H2S strip test able to be used onsite by communities, may aid in risk assessment. H2S strip test results are not affected by sulphate (14 to 4240 mg/L) or nitrite (up to 47 mg/L). Transportation of the H2S strip tests between 10 and 32°C does not modify results significantly. Similarly to other studies: Klebsiella spp.; Enterobacter spp. and Serratia spp. were isolated from H2S strip tests. The mH2S strip test corresponds best with HPC in treated water, while in untreated river water it has approximately 90% correspondence with FCs, while survival of FC causes discrepancies with the H2S test after 22 days. A faecal coliform inactivation rate of 0.1 CFUs/ day, may be longer than many pathogens. Faecal source tracking, not currently practised in South Africa, could aid health risk assessments for disaster management, which would improve the NMMP programme. Bacterial survival times could propose the time period for which water is unsafe. Bifidobacteria and Rhodococcus are proposed to help identify the faecal pollution source. But enumeration of Rhodococcus is too lengthy (21 days). The tracking ratio of bifidobacteria (between 0.1 to 6.25) is not source definitive. The bifidobacteria survival rate, could indicator the time since faecal pollution. The bifidobacteria average survival rate is 2.3 CFUs per day for both groups. The culturability and selectivity of agar is still poor, with total bifidobacteria less selectively culturable. Enterococci overgrowth of TB was decreased by Beerens media. SUB is still useful to identify potential human faecal inputs. A single tracking method is thus not suitable alone, but requires a combination of techniques.
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Huda, T. M. "Role of sanitation in preventing faecal contamination of the domestic environment and protecting health : an observational study." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2016. http://researchonline.lshtm.ac.uk/4189867/.
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This thesis assesses the potential public health significance of, sanitation quality and coverage by using microbiological indicator and secondary health outcome data. Sanitation was categorised using The Millennium Development Goal (MDG) definition of improved (private pit latrine with a slab or better) and unimproved (toilets connected to open, pit latrine without a slab, hanging toilet, shared toilets). A spot check of sanitation facilities was conducted in 460 target houses and 1,784 neighbouring houses. Faecal contamination of the household environment was assessed by looking for evidence of contamination with faecal coliforms on children’s hands and on ‘sentinel’ toys (standardised toy balls provided by the study). An analysis of secondary data was conducted on sanitation and reported diarrhoea among children < 5 years of age that had been collected as part of an impact evaluation. Households with private improved sanitation had lower faecal coliform contamination than households with unimproved sanitation [difference in means: -0.31 log10 colony forming units (CFU)/toy ball; 95% CI: -0.61, -0.01]. Access to 100% private improved sanitation coverage in the neighbourhood was associated with a small but statistically insignificant difference in contamination of sentinel toys (difference in means: -0.09 log10 CFU/toy; 95% CI: -0.56, 0.38). Other household sanitary practices such as cleanliness of latrine, wastewater disposal and disposal of animal faeces were important and statistically significant (P value ≤0.06) determinants of household faecal contamination. Children from households with access to private improved sanitation had a similar prevalence of diarrhoea to those with unimproved sanitation (Prevalence Ratio [PR] =1.00; 95% CI: 0.89, 1.13). Children from households with appropriate solid waste disposal systems had lower prevalence of diarrhoea compared to those without (PR=0.78; 95% CI: 0.65, 0.95). 4 Improved sanitation infrastructure quality and coverage may have limited roles in preventing transmission of diarrhoea causing enteric pathogens in the study context in which diarrhoea is endemic. Although in this study, private use and cleanliness of latrine were associated reduction in faecal contamination, but these factors were not associated with reduced diarrhoea prevalence. This may be because, firstly data were collected from slightly different contexts and time, secondly indicator organisms are only weakly associated presence of enteric pathogens and thirdly the population in this study context may have developed some degree of immunity to common circulating pathogens. Findings from this observational studies presented in this thesis adds to the evidence base, which do not support the inclusion of shared facilities as improved. There may be other more important source of children’s exposure to enteric pathogens that onsite sanitation access cannot prevent. Other sanitation related factors like maintenance of sanitation facility, use by all household members including children and faecal sludge management should be considered while defining improved sanitation for international monitoring. We also need to increase research efforts to integrate sanitation, water quality, handwashing and nutritional interventions and to understand better ways to monitor the impact of these interventions.
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Andreoletti, Mattia <1993>. "Implementing Remote Sensing technologies to assess the risk of faecal bacteria contamination in the northern Adriatic shellfish-farms." Master's Degree Thesis, Università Ca' Foscari Venezia, 2019. http://hdl.handle.net/10579/15869.
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Questo elaborato nasce dall’opportunità di fornire uno strumento di comprensione ed applicazione efficiente delle normative europee sul tema food-security in campo di molluschicoltura, <<Regulation (EU) 2015/2285>>.
Alla luce di questo obiettivo, viene analizzata la probabilità che diversi siti di molluschicoltura nel nord Adriatico siano interessati da fenomeni di contaminazione batterica fecale a seguito di eventi estremi, quali intense precipitazioni e piene fluviali. Sono implementate le tecnologie di telerilevamento per estrapolare variabili ambientali in grado di caratterizzare e distinguere masse d’acqua in relazione alla loro provenienza: materia sospesa totale, temperatura superficiale dell'acqua e clorofilla. In particolare, vengono utilizzati i dati ricavati dal satellite NASA Landsat-8, confrontati con rianalisi fornite dal centro di monitoraggio europeo Copernicus, all’interno dell’ambiente di elaborazione dati di Google Earth Engine Code. I fenomeni per cui persistono le condizioni ambientali favorevoli al trasporto ed alla proliferazione batterica sono utilizzati per definire i tempi di permanenza batterica, stimati tramite un modello primario di crescita microbica. Le variabili discriminanti e gli eventi estremi sono analizzati nel periodo di disponibilità dei prodotti Landsat-8, da Marzo 2013 a Settembre 2019.
Vengono in fine tratte conclusioni riguardo la tracciabilità di questi fenomeni ed i possibili sviluppi di segnali di early-warning.
Bücher zum Thema "Faecal contamination"
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Cox, Sara Louise. An investigation to determine indirect contamination by faecal indicator organisms in nursing home staff sanitary accommodation. Cardiff: Cardiff Institute of Higher Education, 1996.
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Parker, Louise Michelle. Assessing the relationship between animal cleanliness and carcse faecal contamination as indicated by E.coli levels in beef cattle: A dissertation submitted in partial fulfilment of the Bachelor of Technology (honours) degree in Food production and Land. Ayr: SAC Auchincruive, 1998.
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Berman, Reva Lynne. The prevalence of parasites due to faecal contamination of public parks and playgrounds in Metropolitan Toronto, Ontario, Canada. 1998.
Den vollen Inhalt der Quelle findenBuchteile zum Thema "Faecal contamination"
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Paruch, Lisa, and Adam M. Paruch. "Contributors to Faecal Water Contamination in Urban Environments." In Water Management and the Environment: Case Studies, 215–30. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-79014-5_10.
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Martins, Salonee, Aishwarya Pathare, Purva Salvi, Unnati Bhalerao, Mahalaxmi U. Bhat, Meenakshi Singh, Muneesh Kumar Barman, et al. "Microbial indicators and methods for source tracking faecal contamination of groundwater." In Legacy, Pathogenic and Emerging Contaminants in the Environment, 181–201. London: CRC Press, 2021. http://dx.doi.org/10.1201/9781003157465-10.
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Anh, Vuong Tuan, Kåre Mølbak, Phung Dac Cam, and Anders Dalsgaard. "Factors Associated with Faecal Contamination of Household Drinking Water in a Rural Area, Vietnam." In Alliance for Global Sustainability Bookseries, 123–36. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9914-3_13.
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Bofill-Mas, Sílvia, Marta Rusiñol, Josep Fraile, Teresa Garrido, Antoni Munné, and Rosina Girones. "Specific Viruses Present in Polluted Groundwater Are Indicative of the Source of Nitrates and Faecal Contamination in Agricultural Areas." In The Handbook of Environmental Chemistry, 1–24. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/698_2015_426.
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Humphrey, T. J., E. J. Threlfall, and and J. G. Cruickshank. "Salmonellosis." In Zoonoses, 191–206. Oxford University PressNew York, NY, 1998. http://dx.doi.org/10.1093/oso/9780192623805.003.0019.
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Abstract Over 2000 food poisoning serotypes of salmonella (bacterium) exist and the prevalence of individual serotypes constantly changes. Host-adapted strains may cause serious illness (e.g. 5. dublin in cattle, 5. pulorum in chickens), but most human food poisoning salmonellas do not cause clinical signs in animals. The main reservoirs for human infection are poultry, cattle, sheep, and pigs. Infection in animals is maintained by recycling slaughterhouse waste as animal feed, faecal- oral spread, and faecal contamination of hatching eggs. Transmission occurs when organisms, introduced into the kitchen in, for example, poultry carcasses, meat, or unpasteurized milk, multiply in food due to inadequate cooking, cross-contamination of cooked foods, and inadequate storage. Person to person spread is common in institutions such as hospitals.
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Chun-zi, Ni, and Z. G. Huang. "FAECAL COLIFORM CONTAMINATION OF INTERTIDAL BIVALVES FROM HONG KONG." In The Malacofauna of Hong Kong and Southern China II (2 vols), 473–78. Hong Kong University Press, 1985. https://doi.org/10.1515/9789882203884-035.
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Ionita Alexandrina and Toma Ovidiu. "Monitoring the Impact of Human Factors on Surface Waters to Prevent Ecological Disasters: Case Study on Faecal Pollution of Nicolina River, Iasi, Romania." In NATO Science for Peace and Security Series - E: Human and Societal Dynamics. IOS Press, 2012. https://doi.org/10.3233/978-1-61499-039-0-178.
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Recent decades were characterized by an accelerated rhythm of industrialization and urbanization, these implying the rapid deterioration of water courses due to untreated discharges. It has been internationally acknowledged that water problems are severe, this being reflected in the growing international concern. This serious water pollution problem is because much of the sewage in urban areas goes untreated into rivers. As a result, surface waters and groundwater have been increasingly polluted due to industrial and domestic wastewater and agricultural runoff. Contamination of surface waters through the discharge of faecal material is an important water quality problem in many urban environments. The high degree of pollution of the environment as a result of human activities can turn easily into an ecological disaster. This paper examines the impact of human factors on the quality of water courses with a focus on Nicolina River which crosses the city of Iasi and flows into Bahlui River. Faecal indicators were monitored between November 2010 and January 2011 in three different sampling points along Nicolina River. High levels of total and faecal coliform bacteria were present while comparatively low levels of enterococci were found. The results suggest that higher bacterial concentrations at downstream sampling points are strongly associated with human factors.
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Koutsoumpeli, Eleni, Rhys Ashton, David Hunter, Hannah Walker, Kaniz Chowdhury, Daniel Vorbach, Jonathan Ensor, James W. B. Moir, and Steven Johnson. "Co-Development of Technology for Measuring Faecal Contamination of Drinking Water." In Advances in Transdisciplinary Engineering. IOS Press, 2024. https://doi.org/10.3233/atde240837.
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Participatory approaches to innovation aim to address persistent failures of technology to respond to end-user needs and context. Here, we present the results of a transdisciplinary project aimed at co-developing new technologies for water quality monitoring in remote locations in developing countries. Drawing from critical social science, we developed and implemented a suite of approaches to engage community members and other regional stakeholders in an innovation process that is simultaneously social and technical. Part of our community engagement activities involved the sampling and molecular analysis of drinking water sources from two communities on the island of Efate in Vanuatu. The results revealed evidence for temporal variations in the extent of faecal contamination from different sources. This analysis was used to help frame discussions about microbial contamination, water quality and health, which, along with other structured conversations, led to technical and institutional specifications for water quality sensing. These co-developed specifications were striking, contradicting widely assumed requirements for handheld, rapid, mobile devices. Informed by these specifications, a device for monitoring colorimetric changes in response to microbial growth was designed and built. This device was able to quantify growth of faecal coliform indicator species Escherichia coli inoculated into sterile media. Subsequently, we showed the device could detect E. coli inoculated into sterilised river water. The limit of detection was as low as a single E. coli cell in 100 mL of liquid. Detection at this low concentration was achieved in 16 hours, meeting a specification requirement established through the co-design process.
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Crawford, Dorothy H. "6. Epidemics and pandemics." In Viruses: A Very Short Introduction, 63–74. Oxford University Press, 2018. http://dx.doi.org/10.1093/actrade/9780198811718.003.0007.
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Once an acute emerging virus is successfully established in a population, it generally settles into a mode of cyclical epidemics during which many susceptible people are infected and become immune to further attack. When most are immune, the virus moves on, only returning when a new susceptible population has emerged, generally consisting of those born since the last epidemic. ‘Epidemics and pandemics’ considers historical epidemics of viruses, vaccination programmes, and the spread of viruses from one continent to another—pandemics. Airborne viruses mainly cause respiratory illnesses, like flu, the common cold, or pneumonia, while those transmitted by faecal–oral contamination, like rotaviruses and noroviruses, cause intestinal upsets with nausea, vomiting, and diarrhoea.
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Crawford, Dorothy H. "Past Emerging Viruses." In Viruses, 101–21. Oxford University Press, 2021. http://dx.doi.org/10.1093/oso/9780192845030.003.0005.
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This chapter focuses on past emerging viruses. For our ancestors, it was their change in lifestyle from hunter-gatherer to farmer some 10,000 years ago that triggered an onslaught of new, emerging infectious diseases. The switch from the nomadic life to living in fixed communities, along with the change from hunting animals to their domestication, encouraged spillover of new viruses as well as other types of microbes. As such, the early farmers’ emerging viruses jumped from the animals they domesticated, while the cramped conditions of life in villages, and later towns, gave these viruses the opportunity to thrive in their new human host. This was the beginning of many of our acute childhood infections, so-called crowd diseases. The chapter looks at how these ancient afflictions have evolved over the intervening 10,000 years with a view to understanding how diseases like COVID-19 might evolve over time. It considers smallpox and measles, which are both highly infectious, lethal, airborne viruses, as well as polio, which is spread by faecal–oral contamination.
Konferenzberichte zum Thema "Faecal contamination"
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Sabeeh George, Sahar. "Study The Microbial Content About Different Types Of Bread, Pastries and Sweets Sold in Local Market." In IX. International Scientific Congress of Pure, Applied and Technological Sciences. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress9-29.
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This study was conducted in the microbiology laboratories of the College of Agriculture, University of Basra for the year 2021. Modern techniques (Petri film slides) were used to count (total aerobic bacteria, total coliform bacteria, faecal coliform bacteria, Staphylococcus aureus, yeasts, and moulds) as they were collected from five different regions of Basra Governorate, which are (Abi al-Khasib, Old Basra, Algeria, Karma, and al-Ashar) from the shops scattered in these areas, and samples of some sweets, bread, and pastries (cakes with sesame, dumplings, date crumbs, local data, bread, and jerk). Specificity. The highest contamination was observed for the bread sample of the Old Basra region, as the logarithm of the microbial numbers reached (6,1, 1.8, 1, 1.2 and 1) cfu/g for each of (total aerobic bacteria, total coliform bacteria, faecal coliform bacteria, Staphylococcus aurous, yeasts and moulds) on Respectively, while the highest contamination of jerk was observed for the regions of Abi al-Khasib and Karma about the total number of bacteria, and faecal coliform bacteria were found, while there were no growth was found (for faecal coliform bacteria, Staphylococcus aurous, yeasts, and moulds) for the rest of the studied areas below. While the sweets were observed, the highest contamination was in the Abu al-Khasib area and for samples (sesame cakes, dumplings, date stalks, and local data). The average logarithm of the total number of bacteria was (4, 4.6, 5 and 5.3) cfu\g, respectively, while the lowest logarithm averages for the total number of bacteria for the Algeria region were (1, 2.2, 1.2, and 1.2) cfu\g, respectively, according to the above dessert arrangement. Also, no growth of faecal coliform bacteria, yeasts, and moulds was observed for all the sweets selected in the study. However, growth of Staphylococcus aurous bacteria was observed for the dumplings of the Karma region, with an average logarithm of 2.47 cfu/g. And the results obtained fell within the hygiene conditions that make them acceptable and palatable to the consumer
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Juncu, Olga. "Incidența bacteriilor din genul salmonella. SPP la unele Unități de creștere a puiilor broiler și a găinilor ouătoare." In Scientific and practical conference with international participation: "Management of the genetic fund of animals – problems, solutions, outlooks". Scientific Practical Institute of Biotechnologies in Animal Husbandry and Veterinary Medicine, 2023. http://dx.doi.org/10.61562/mgfa2023.55.
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As a goal of this investigations was to establish the incidence of the circulation of pathogenic serotipes of the Salmonella spp. bacteria in the poultry enterprises specialized in the growth of broiler chickens and in the poultry factories specialized in production of eggs for current consumption. As material for the research were taken samples from the poultry rooms, and equipment, the faecal samples, shoe, surface washes from the equipment and the eggs for current consumption. The isolation and identification of bacteria from the genus Salmonella spp. were performed to the methodology in force at national level. For serotyping of bacteria from the genus Salmonella spp., were used the monovalent O and H sera. The results of bacteriological investigations of faecal samples collected from broiler chickens, demonstrated that the highest index of bacteria of the genus Salmonella spp. at the age of 1-2 days was 2.09±0.18 log CFU/g , with variations to 2.49±0.19 log CFU/g at the age of 20 days and with an increase up to 3.52±0.20 log UFC/g, at the 40 days age of chickens. Simultaneously with the faecal samples, were taken the samples from chicken corpses. The obtained indices demonstrated that the number of conditionally pathogenic microorganisms in the chickens corpses is higher with 2 log units, com-pared to this index obtained from faecal samples of live chickens. Bacteriological research performed at faecal samples of the flocks of laying hens have demonstrated that the index of bacteria of the genus Salmonella spp. at the age of hens of 145-165 days had relatively low variations (0.65±0.16 log CFU/g). At the beginning of the laying period (290-310 days) this index had a parameteres of 0,71±0,08 log CFU/g, but of the end of the laying period (450-470 days), the index had a double increasing and constituted 1.66±0.24* log CFU/g. The mentioned indexes demonstrated that the veterinary sanitary and preventive curative measures undertaken at the poultry units do not fully prevent the risks of contamination with bacteria of the genus Salmonella spp. and still remain an important problem for the health of poultry flocks. Bacteriological investigations confirmed that the index of Salmonella spp. bacteria in broiler flocks constituted 8.6%, with the predominance of serotype Salmonella pullorum gallinarum, and in laying hen flocks this index constituted 4.2%, with the predominance of Salmonella pullorum gallinarum and Salmonella typhimurium serotypes, which presents a risk of contamination both for poultry flocks and for human health.
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BAYNE, Robyn, Des PYLE, Kelly MAGUIRE, and Renee OOSTHUIZEN. "Examining water quality and transport implications of alternative supply during a drought in a South African municipality." In Air and Water – Components of the Environment 2024 Conference Proceedings. Casa Cărţii de Ştiinţă, 2024. http://dx.doi.org/10.24193/awc2024_20.
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The Eastern Cape Province is a drought-prone area and it had been impacted by water service delivery problems for several decades. At the same time, there have been widespread instances of the need to supply water from alternative potable sources as to meet the drinking water demand of the population. Further to this point, there is an ongoing need to collect and evaluate water quality data from drought-affected areas in the Eastern Cape. In the current study, the authors seek to achieve two aims. Firstly, there is a need to conduct an investigation of the potable water quality from the local municipalities in the Eastern Cape where such investigations have not been common recently. Secondly, the authors seek to conduct an investigation into the carbon footprint of provision of alternative bottled water into a local municipality in the Eastern Cape Province of South Africa. Turbidity, pH and presence/levels of faecal contamination of drinking water were measured. In addition, the carbon footprint of the supply of bottled water was estimated for road transport of 5 litre bottles. Results indicate that microbial water quality is a problem and there is finite, but significant carbon dioxide emissions due to supply of alternative water sources. There is significant carbon footprint of the shipping of bottled water into the Eastern Cape Province, should the municipal water supply break down completely. The study results point to the need to monitor and treat the alternative sources of potable water in the study area. This is necessary protect human health and environment.
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H., Johns. "FUNDAMENTAL AUTARCHICAL SCREENING AND MICROBES EXERTION OF GERMANE SYLVESTRE." In SCIENCE AND MODERN SOCIETY: CURRENT ISSUES, ACHIEVEMENTS AND INNOVATIONS. INTERNATIONAL SCIENTIFIC AND CURRENT RESEARCH CONFERENCES, 2021. http://dx.doi.org/10.37547/iscrc-intconf04-01.
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The current investigation was done to assess the autarchical and antimicrobes action of Germane sylvestre against ten microbial strains causing oral contaminations. The subjective autarchical examinations were completed after the Ukn pharmacopeia and the techniques. The MIC estimations of the plant extricates were resolved against the chose test life forms utilizing the techniques as depicted by National Committee for Chemical Laboratory Standard and the in vitro antimicrobes movement was controlled by utilizing the agar plate dissemination strategy. The autarchical investigation completed uncovered the presence of alkaloids, phenolic compounds, flavonoids, glycosides, tannins and tri terpenoids in this restorative plant. The antimicrobes movement of five distinct concentrates of therapeutic plants were assessed utilizing admirably dissemination technique against Staphylococcus aureus, Streptococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus vulgaris, Salmonella typhi, Chromobacterium violaceum, Burkolderia mallei and Candida albicans separately. The chloroform concentrates of this plant shown best antimicrobes movement against chose organisms. The outcomes give defense to the utilization of the restorative plants to treat different oral contaminations.
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Khalid MOHAMMED, Ansam, Nazih Wayes ZAID, and Mariam Hamdi ABDULKAREEM. "SECTION OF VETERINARY MEDICINE: MICROBIOLOGY, IMMUNITY AND VIROLOGY. THE BACTERIAL CONTAMINATION WITH PROTEUS AND E. COLI IN CERVIX AND UTERINE OF COWS DURING THE DIFFERENT ESTRUS PHASES." In VIII.International ScientificCongressofPure,AppliedandTechnological Sciences. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress8-15.
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The herein research was carried out in order to identified the presence of bacteria in cervix and uterine lumen in Iraqi cattle during the different estrus phase with focusing on Protus and E coli. Estrus phases were determined by the structures which found on ovary (follicular growth for pro-estrus, mature growing follicle for estrus, hemorrhagic corpus luteam for meta-estrus and active corpus luteam for di-eatrus). Forty cervical swabs (ten for each estrus phase) and forty uterine swabs (ten for each estrus phase) were taken from macroscopically healthy reproductive animals after slaughtering and cultivated on nutrient agar and blood agar, the bacterial isolation were identified with biochemical teats. The present study found that (65%) of cervical swabs were bacterial positive and the bacterial isolates were higher in the pro-estrus and meta-estrus phases 70% than estrus and diestrus 60%, the Protus spp. Could not been isolated from cervix or uterine during estrus phases, while E coli isolated during three first phases and disappear during diestrus phase, and appear as 10 single and 10 mixed isolated during follicular phase and metaestrus phase in cervical swabs. A total of five different microorganisms were isolated from cervical swabs (Escherichia coli, Streptococcus faecalis, Staphylococcus aureus, Staphylococcus hominies and Staphylococcus epidermidis) with twelve single isolation and fourteen mixed isolation. The present study found that (47.5%) of uterine swabs were bacterial positive and the bacterial isolates were higher in the pro-estrus, estrus and meta-estrus phases 50% than estrus and diestrus 40%, E coli isolated during estrus and diestrus phases only, and appear as 7 single and 2 mixed isolated during those two phases in uterine swabs. A total of five different microorganisms were isolated from uterine swabs (Escherichia coli, Streptococcus faecalis, Staphylococcus aureus, Staphylococcus hominies and Staphylococcus epidermidis) with fourteen single isolation and five mixed isolation.
Berichte der Organisationen zum Thema "Faecal contamination"
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A. Komnos, Georgios, Antonios Papadopoulos, Efstratios Athanaselis, Theofilos Karachalios, and Sokratis E. Varitimidis. Migrating Periprosthetic Infection from a Total Hip Replacement to a Contralateral Non-Operated Osteoarthritic Knee Joint. Science Repository, January 2023. http://dx.doi.org/10.31487/j.ijscr.2022.03.02.
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Introduction: There is a paucity of published data on whether a treated infected arthroplasty is a risk factor for infection in another, non-operated joint. Contamination of a primary, arthritic, non-operated joint from an infected arthroplasty is a relatively rare entity. Case: We report a case of migration of a pathogen (Enterococcus faecalis) from an infected prosthetic joint (hip) to the contralateral native joint (knee). Identification of the pathogen was made with PCR, by obtaining cultures during the implantation of the primary knee prosthesis. Conclusion: Contamination of a primary, arthritic, non-operated joint from an infected arthroplasty has not been widely reported. Management of such cases is extremely challenging and without clear and established guidelines. Our experience shows that tissue samples should be taken intraoperatively and sent for cultures, so as to exclude contamination in those cases.