Zeitschriftenartikel: „Extrakce EKG“

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ADAMIEC, J., M. DOLEŽAL, K. MÍKOVÁ, and J. DAVÍDEK. "Changes in Egg Volatiles during Storage." Czech Journal of Food Sciences 20, No. 2 (2011): 79–82. http://dx.doi.org/10.17221/3515-cjfs.

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The quality of eggs is tightly associated with freshness. New possibilities for the determination of egg freshness were studied. The volatile compounds of eggs and their changes during storage were followed. Three methods for extraction of volatiles were compared: dynamic headspace (Purge and Trap), static headspace (Solid Phase of Microextraction &ndash; SPME) and extraction according to Likens-Nickerson by simultaneous distillation-extraction (SDE) with diethyl ether as organic solvent. The extracts were analysed by GC/FID. The volatiles in an extract obtained by SDE method were identified by GC-MS. The extract includes aldehydes, alcohols, acids and esters. The volatiles in an extract obtained by SPME and Purge and Trap have not been identified until now. The changes in volatiles during storage of eggs using the above mentioned methods were studied.

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Santoso, U., and Y. Fenita. "THE EFFECT OF Sauropus androgynus LEAF EXTRACT ON PERFORMANCE, EGG QUALITY AND CHEMICAL COMPOSITION OF EGGS." Journal of the Indonesian Tropical Animal Agriculture 41, no. 3 (2016): 125–34. http://dx.doi.org/10.14710/jitaa.41.3.125-134.

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The purpose of this research was to produce the enriched eggs, but low in cholesterol content. Forty eight layer chickens aged 72 weeks (strain Dekalb Warren) were distributed into six treatment groups. One group of laying hens was fed diets without Sauropus androgynusleaf extract (SALE) as the control, and another five groups were fed diet supplemented to 9 g SALE, 18 g SALE, 27 g SALE, 36 g SALE, and 45 g SALE/kg diet, respectively. Completely randomized design was used in the present study.The experimental results showed that SALE supplementation had no effect on egg production, feed intake, feed conversion ratio, egg weight, yolk weight, albumen weight, shell egg, egg index, eggshell thickness, and yolk index (P>0.05), but significantly affected Haugh Unit, albumen index, yolk color, egg odor, egg taste, and yolk color (P<0.05). The protein, fat, calsium, phosphor, kaliumand iron contents of eggs did not change (P>0.05), but the cholesterol content of egg was significantly reduced (P<0.05). In conclusion, the supplementation of SALE did not enhance performance, but it improve egg quality and its chemical composition.

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Barrows, John K., and David T. Long. "Cell-free transcription in Xenopus egg extract." Journal of Biological Chemistry 294, no. 51 (2019): 19645–54. http://dx.doi.org/10.1074/jbc.ra119.011350.

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Groen, Aaron C., Margaret Coughlin, and Timothy J. Mitchison. "Microtubule assembly in meiotic extract requires glycogen." Molecular Biology of the Cell 22, no. 17 (2011): 3139–51. http://dx.doi.org/10.1091/mbc.e11-02-0158.

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The assembly of microtubules during mitosis requires many identified components, such as γ-tubulin ring complex (γ-TuRC), components of the Ran pathway (e.g., TPX2, HuRP, and Rae1), and XMAP215/chTOG. However, it is far from clear how these factors function together or whether more factors exist. In this study, we used biochemistry to attempt to identify active microtubule nucleation protein complexes from Xenopus meiotic egg extracts. Unexpectedly, we found both microtubule assembly and bipolar spindle assembly required glycogen, which acted both as a crowding agent and as metabolic source glucose. By also reconstituting microtubule assembly in clarified extracts, we showed microtubule assembly does not require ribosomes, mitochondria, or membranes. Our clarified extracts will provide a powerful tool for activity-based biochemical fractionations for microtubule assembly.

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Makal, Henny V. G., and Deflly A. S. Turang. "PEMANFAATAN EKSTRAK KASAR BATANG SERAI UNTUK PENGENDALIAN LARVA Crosidolomia binotalis Zell. PADA TANAMAN KUBIS." EUGENIA 17, no. 1 (2011): 16. http://dx.doi.org/10.35791/eug.17.1.2011.95.

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Theresearch wasaimed to assess effect of some concentrations of lemon grass stem extract to C.binotalis larvae. The advantage of the research was to give information the use lemon grass stemextract as a botanical insecticide to control C. binotalis larvae. The result showed that after applicationof the lemon grass stem extract, the C. binotalis larvae showed symptoms such as inactive of eating,inactive of movement and eventually death of larvae C. binotalis.The death of larvae was showingchanging of color at dorsal and ventral. The color at the dorsal became pale yellow and the color atventral turned to light brown.The death larvae subsequently were hardened and the entire body thelarvae were blackish brown.The percentage of C. binotalis larvae mortality increased when theconcentration of lemon grass stem extract was also increased.Concentration 80 g/50 ml resulted 95 %death of total tested larvae. It could be concluded thatlemon grass stem extract can be used to controllarvae of C.binotalis on cabbage. ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh beberapa konsentrasi ekstrak batang seraiterhadap larva C. binotalis. Manfaat dari penelitian ini adalah untuk memberikan informasi tentangpenggunaan ekstrak batang serai sebagai insektisida botani untuk mengendalikan larva C. binotalis.Gejala kematian larva C. binotalis setelah aplikasi ekstrak batang serai pada awalnya larva kelihatangelisah, tidak aktif makan kemudian menjadi tidak aktif bergerak dan akhirnya mati. Larva yang matiterjadi perubahan warna, pada bagian dorsal berwarna kuning pucat dan bagian ventral berwarnacoklat muda dan lama kelamaan seluruh tubuh mengeras dan berwarna coklat kehitaman. Hasilpenelitian menunjukan bahwa persentase mortalitas larva C binotalis meningkat seiring denganmeningkatnya konsentrasi ekstrak batang serai yang diaplikasikan. Pada konsentrasi ekstrak batangserai 80g/ml menyebabkan mortalitas sebesar 95% dari keseluruhan larva uji. Berdasarkan hasil ini,ekstrak batang serai dapat digunakan untuk mengendalikan larva C. binotalis pada kubis.

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Marchon, Niyan, and Gourish Naik. "Sharp Filters To Extract Absence Seizures EEG Signals." International Journal of Computer Sciences and Engineering 06, no. 10 (2018): 37–41. http://dx.doi.org/10.26438/ijcse/v6si10.3741.

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Broadus, Matthew R., and Ethan Lee. "Chemical Screening Using Cell-Free Xenopus Egg Extract." Cold Spring Harbor Protocols 2018, no. 8 (2018): pdb.prot098277. http://dx.doi.org/10.1101/pdb.prot098277.

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Mei, Chunli, Xuelu Han, Jing Zhang, Ling Gao, and Huimin Liu. "Neuroprotective Effects of Ginkgo Biloba Extract (GbE) on Oxygen-Glucose Deprivation (OGD) in PC12 Cells." Engineering 05, no. 10 (2013): 142–45. http://dx.doi.org/10.4236/eng.2013.510b030.

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Makita, Masashi, Kiyoshi Shiroishi, and Hiroaki Fujii. "Examination of Experimental Method to Extract Characteristics of Lower Limb Movement in Emergency Braking Operation." Engineering 12, no. 07 (2020): 457–65. http://dx.doi.org/10.4236/eng.2020.127032.

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Seok, Woojoon, Minsoo Yeo, Jiwoo You, et al. "Optimal Feature Search for Vigilance Estimation Using Deep Reinforcement Learning." Electronics 9, no. 1 (2020): 142. http://dx.doi.org/10.3390/electronics9010142.

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A low level of vigilance is one of the main reasons for traffic and industrial accidents. We conducted experiments to evoke the low level of vigilance and record physiological data through single-channel electroencephalogram (EEG) and electrocardiogram (ECG) measurements. In this study, a deep Q-network (DQN) algorithm was designed, using conventional feature engineering and deep convolutional neural network (CNN) methods, to extract the optimal features. The DQN yielded the optimal features: two CNN features from ECG and two conventional features from EEG. The ECG features were more significant for tracking the transitions within the alertness continuum with the DQN. The classification was performed with a small number of features, and the results were similar to those from using all of the features. This suggests that the DQN could be applied to investigating biomarkers for physiological responses and optimizing the classification system to reduce the input resources.

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Apriliana, Kadek Soma, Wayan Bebas, and I. Gusti Ngurah Bagus Trilaksana. "Mempertahankan Motilitas dan Viabilitas Spermatozoa Babi dalam Pengencer Air Kelapa Kuning Telur Bebek dengan Pengimbuhan Sari Wortel." Indonesia Medicus Veterinus 10, no. 3 (2021): 409–19. http://dx.doi.org/10.19087/imv.2021.10.3.409.

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Penelitian ini dilakukan untuk mendapatkan pengencer semen babi yang murah, berkualitas, dan mudah dibuat dengan menggunakan air kelapa dan kuning telur yang ditambahkan sari wortel. Penelitian ini menggunakan satu ekor babi Landrace jantan dan penampungan semen dilakukan dengan metode massage. Penelitian ini menggunakan Rancangan Acak Lengkap, terdiri dari dua perlakuan, yaitu perlakuan satu: semen babi diencerkan dengan pengencer air kelapa kuning telur bebek; dan perlakuan dua: semen babi diencerkan dengan air kelapa kuning telur bebek dengan penambahan sari wortel 1%. Semen yang telah diencerkan disimpan pada suhu 18-20ºC dengan lama penyimpanan masing-masing: 0 jam, 4 jam, 8 jam, 12 jam, 16 jam, 20 jam, 24 jam, 28 jam dan 32 jam. Masing-masing perlakuan diulang sebanyak tiga kali, sehingga jumlah sampel yang digunakan adalah 27 sampel untuk setiap perlakuan. Selama penyimpanan dilakukan pengamatan terhadap motilitas dan viabilitas spermatozoa. Data yang diperoleh dianalisis dengan uji sidik ragam, dan bila terdapat perbedaan yang bermakna maka dilanjutkan menggunakan uji Duncan. Hasil penelitian menunjukkan lama simpan semen dalam pengencer air kelapa kuning telur bebek dengan penambahan sari wortel berpengaruh nyata terhadap motilitas dan viabilitas spermatozoa babi Landrace. Penambahan sari wortel mampu mempertahankan motilitas progresif selama 20 jam dengan persentase 41,39%, sedangkan viabilitas dipertahankan selama 28 jam dengan persentase 45,45%.

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Li, C. J., J. A. Bogan, D. A. Natale, and M. L. DePamphilis. "Selective activation of pre-replication complexes in vitro at specific sites in mammalian nuclei." Journal of Cell Science 113, no. 5 (2000): 887–98. http://dx.doi.org/10.1242/jcs.113.5.887.

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As the first step in determining whether or not pre-replication complexes are assembled at specific sites along mammalian chromosomes, nuclei from G(1)-phase hamster cells were incubated briefly in Xenopus egg extract in order to initiate DNA replication. Most of the nascent DNA consisted of RNA-primed DNA chains 0.5 to 2 kb in length, and its origins in the DHFR gene region were mapped using both the early labeled fragment assay and the nascent strand abundance assay. The results revealed three important features of mammalian replication origins. First, Xenopus egg extract can selectively activate the same origins of bi-directional replication (e.g. ori-beta) and (beta') that are used by hamster cells in vivo. Previous reports of a broad peak of nascent DNA centered at ori-(beta/(beta)' appeared to result from the use of aphidicolin to synchronize nuclei and from prolonged exposure of nuclei to egg extracts. Second, these sites were not present until late G(1)-phase of the cell division cycle, and their appearance did not depend on the presence of Xenopus Orc proteins. Therefore, hamster pre-replication complexes appear to be assembled at specific chromosomal sites during G(1)-phase. Third, selective activation of ori-(beta) in late G(1)-nuclei depended on the ratio of Xenopus egg extract to nuclei, revealing that epigenetic parameters such as the ratio of initiation factors to DNA substrate could determine the number of origins activated.

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Marchon, Niyan, Gourish Naik, and Radhakrishna Pai. "ECG Electrode Configuration to Extract Real Time FECG Signals." Procedia Computer Science 125 (2018): 501–8. http://dx.doi.org/10.1016/j.procs.2017.12.065.

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Higa, Meda M., Katharine S. Ullman, and Amy J. Prunuske. "Studying nuclear disassembly in vitro using Xenopus egg extract." Methods 39, no. 4 (2006): 284–90. http://dx.doi.org/10.1016/j.ymeth.2006.06.004.

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Sannino, Vincenzo, Arun M. Kolinjivadi, Giorgio Baldi, and Vincenzo Costanzo. "Studying essential DNA metabolism proteins in Xenopus egg extract." International Journal of Developmental Biology 60, no. 7-8-9 (2016): 221–27. http://dx.doi.org/10.1387/ijdb.160103vc.

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Tirnauer, Jennifer S., E. D. Salmon, and Timothy J. Mitchison. "Microtubule Plus-End Dynamics in Xenopus Egg Extract Spindles." Molecular Biology of the Cell 15, no. 4 (2004): 1776–84. http://dx.doi.org/10.1091/mbc.e03-11-0824.

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Microtubule dynamics underlie spindle assembly, yet we do not know how the spindle environment affects these dynamics. We developed methods for measuring two key parameters of microtubule plus-end dynamic instability in Xenopus egg extract spindles. To measure plus-end polymerization rates and localize growing plus ends, we used fluorescence confocal imaging of EB1. This revealed plus-end polymerization throughout the spindle at ∼11 μm/min, similar to astral microtubules, suggesting polymerization velocity is not regionally regulated by the spindle. The ratio of EB1 to microtubule fluorescence revealed an enrichment of polymerizing ends near the spindle middle, indicating enhanced nucleation or rescue there. We measured depolymerization rates by creating a front of synchronized depolymerization in spindles severed with microneedles. This front could be tracked by polarization and fluorescence microscopy as it advanced from each cut edge toward the associated pole. Both imaging modalities revealed rapid depolymerization (∼30 μm/min) superimposed on a subset of microtubules stable to depolymerization. Larger spindle fragments contained a higher percentage of stable microtubules, which we believe were oriented with their minus ends facing the cut. Depolymerization was blocked by the potent microtubule stabilizing agent hexylene glycol, but was unaffected by α-MCAK antibody and AMPPNP, which block catastrophe and kinesin motility, respectively. These measurements move us closer to understanding the complete life history of a spindle microtubule.

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Chen, Pan, and Daniel L. Levy. "Nucleus Assembly and Import in Xenopus laevis Egg Extract." Cold Spring Harbor Protocols 2018, no. 6 (2018): pdb.prot097196. http://dx.doi.org/10.1101/pdb.prot097196.

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Flores Servin, Julio C., and Aaron F. Straight. "Centromere and Kinetochore Assembly in Xenopus laevis Egg Extract." Cold Spring Harbor Protocols 2018, no. 9 (2018): pdb.prot102509. http://dx.doi.org/10.1101/pdb.prot102509.

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Dabauvalle, Marie-Christine, and Ulrich Scheer. "Assembly of nuclear pore complexes in Xenopus egg extract." Biology of the Cell 72, no. 1-2 (1991): 25–29. http://dx.doi.org/10.1016/0248-4900(91)90074-w.

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Priharyanthi, Luh Komang Ayu Puteri, Wayan Bebas, and I. Gusti Ngurah Bagus Trilaksana. "Ekstrak Daun Kelor Dapat Mempertahankan Motilitas Progresif dan Viabilitas Spermatozoa Babi dalam Pengencer Air Kelapa Kuning Telur." Indonesia Medicus Veterinus 10, no. 3 (2021): 389–98. http://dx.doi.org/10.19087/imv.2021.10.3.389.

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Penelitian ini bertujuan untuk mengetahui lama penyimpanan semen babi landrace yang disimpan dalam pengencer air kelapa kuning telur dengan ekstrak daun kelor. Penelitian ini menggunakan seekor babi landrace jantan. Penampungan semen dilakukan dengan metode massage. Penelitian ini menggunakan Rancangan Acak Lengkap yang terdiri dari dua perlakuan yaitu kontrol: semen babi diencerkan dengan pengencer air kelapa kuning telur ayam dengan penambahan ekstrak daun kelor 5%. Semen yang telah diencerkan disimpan pada suhu 18-20oC dengan lama penyimpanan masing-masing 0 jam, 4 jam, 8 jam, 12 jam, 16 jam, 20 jam, 24 jam, 28 jam, dan 32 jam. Masing-masing perlakuan diulang sebanyak tiga kali, sehingga jumlah sampel yang digunakan adalah 27 sampel untuk setiap perlakuan. Selama penyimpanan dilakukan pengamatan terhadap motilitas dan viabilitas spermatozoa. Data yang telah diperoleh dianalisis dengan uji Sidik Ragam, apabila terdapat perbedaan yang nyata antar perlakuan maka uji dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan lama penyimpanan semen berpengaruh nyata terhadap motilitas progresif dengan persentase sebesar 55,10% dan viabilitas dengan persentase sebesar 56,58%. Dari hasil penelitian dapat disimpulkan bahwa penyimpanan semen babi Landrace dalam pengencer air kelapa kuning telur dengan ekstrak daun kelor masih layak digunakan sampai 16 jam penyimpanan.

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Thiéry, Denis, Bruno Gabel, Pavel Farkas, and Marc Jarry. "Egg dispersion in codling moth: Influence of egg extract and of its fatty acid constituents." Journal of Chemical Ecology 21, no. 12 (1995): 2015–26. http://dx.doi.org/10.1007/bf02033859.

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Qiao, Xiao Yan, and Jia Hui Peng. "P300 Feature Extraction of Visual and Auditory Evoked EEG Signal." Applied Mechanics and Materials 490-491 (January 2014): 1374–77. http://dx.doi.org/10.4028/www.scientific.net/amm.490-491.1374.

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It is a significant issue to accurately and quickly extract brain evoked potentials under strong noise in the research of brain-computer interface technology. Considering the non-stationary and nonlinearity of the electroencephalogram (EEG) signal, the method of wavelet transform is adopted to extract P300 feature from visual, auditory and visual-auditory evoked EEG signal. Firstly, the imperative pretreatment to EEG acquisition signals was performed. Secondly, respectivly obtained approximate and detail coefficients of each layer, by decomposing the pretreated signals for five layers using wavelet transform. Finally, the approximate coefficients of the fifth layer were reconstructed to extract P300 feature. The results have shown that the method can effectively extract the P300 feature under the different visual-auditory stimulation modes and lay a foundation for processing visual-auditory evoked EEG signals under the different mental tasks.

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Labhart, Paul. "Ku-Dependent Nonhomologous DNA End Joining inXenopus Egg Extracts." Molecular and Cellular Biology 19, no. 4 (1999): 2585–93. http://dx.doi.org/10.1128/mcb.19.4.2585.

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ABSTRACT An extract from activated Xenopus eggs joins both matching and nonmatching ends of exogenous linear DNA substrates with high efficiency and fidelity (P. Pfeiffer and W. Vielmetter, Nucleic Acids Res. 16:907–924, 1988). In mammalian cells, such nonhomologous end joining (NHEJ) is known to require the Ku heterodimer, a component of DNA-dependent protein kinase. Here I investigated whether Ku is also required for the in vitro reaction in the egg extract. Immunological assays indicate that Ku is very abundant in the extract. I found that all NHEJ was inhibited by autoantibodies against Ku and that NHEJ between certain combinations of DNA ends was also decreased after immunodepletion of Ku from the extract. The formation of a joint between a DNA end with a 5′-protruding single strand (PSS) and an end with a 3′-PSS, between two ends with 3′-PSS, and between two blunt ends was most Ku dependent. On the other hand, NHEJ between two DNA ends bearing 5′-PSS was Ku independent. These results show that theXenopus cell-free system will be useful to biochemically dissect the role of Ku in eukaryotic NHEJ.

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Patil, Sureshgouda, Ram Singh, and S. B. Kalidhar. "Effect of Methanolic Extract and its Fractions of Karanj (Pongamia pinnata) Seeds on Oviposition and Egg Hatching of Plutella xylostella (Lepidoptera: Yponomeutidae)." Entomologia Generalis 27, no. 1 (2004): 25–33. http://dx.doi.org/10.1127/entom.gen/27/2004/25.

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Deswati, Dyta Andri, and Maryati Maryati. "Swimming endurance of mice administered black tea extract and chicken egg powder." Jurnal Penelitian Teh dan Kina 20, no. 1 (2017): 30. http://dx.doi.org/10.22302/pptk.jur.jptk.v20i1.128.

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Research of swimming endurance on mice administered black tea extract and chicken egg powder was carried out. The aim of this research was to evalute natatory exhaustion mice administered black tea extract, chicken egg powder and its combinated. The result of this research shown that mice administered of black tea extract and chicken egg powder have a swimming endurance 3.25 minutes and 3.01 minutes. Mice which administered combination of black tea extract and chicken egg powder have a swimming endurance 4.80 minutes. Generally, mice which administered combination of black tea extract and chicken egg powder have a swimming endurance more better than mice which administered of black tea extract and chicken egg powder individually.

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Majoros, Tamás, Balázs Ujvári, and Stefan Oniga. "EEG data processing with neural network." Carpathian Journal of Electronic and Computer Engineering 12, no. 2 (2019): 33–36. http://dx.doi.org/10.2478/cjece-2019-0014.

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Abstract Machine-learning techniques allow to extract information from electroencephalographic (EEG) recordings of brain activity. By processing the measurement results of a publicly available EEG dataset, we were able to obtain information that could be used to train a feedforward neural network to classify two types of volunteer activities with high efficiency.

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Yildiz, G., P. Sacakli, and T. Gungor. "The effect of dietary Jerusalem artichoke (Helianthus tuberosus L.) on performance, egg quality characteristics and egg cholesterol content in laying hens." Czech Journal of Animal Science 51, No. 8 (2011): 349–54. http://dx.doi.org/10.17221/3950-cjas.

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This research was carried out to determine the effect of Jerusalem artichoke (JA) with or without 5, 10% vetch (V) supplementation on performance, egg quality characteristics and egg cholesterol content. In the study, seventy-five 25 weeks-old commercial white laying hens were randomly divided into one control and 4 treatment groups each containing 15 hens. Control group was fed basal diet without JA or V. Treatment group 1, 2, 3 and 4 were fed diets containing 5% V, 5% JA, 5% JA + 5% V and 10% JA + 10% V, respectively. The feeding period lasted 16 weeks. In the study, it was determined that dried-ground JA contained dry matter, crude protein, ether extract, crude fibre, crude ash, inulin and metabolizable energy (ME) at the level of 93.30, 10.02, 0.36, 5.64, 9.05, 15.80% and 3 060 kcal/kg, respectively. At the end of the study, live weight was lower by 4.36&ndash;10.09% in the treatment group 10% JA + 10% V compared with the other groups, but feed efficiency was improved in this group. There were statistically significant differences between the groups in egg quality characteristics (P &lt; 0.05, P &lt; 0.001). Egg production was not affected by supplementation of 5, 10% JA with or without 5, 10% V supplementation. Egg yolk cholesterol and total cholesterol content were not different in the groups (P &gt; 0.05). As a result, the addition of JA with or without V has no adverse effect on performance and egg quality in hens. &nbsp;

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Bell, P., M. C. Dabauvalle, and U. Scheer. "In vitro assembly of prenucleolar bodies in Xenopus egg extract." Journal of Cell Biology 118, no. 6 (1992): 1297–304. http://dx.doi.org/10.1083/jcb.118.6.1297.

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Nuclei assembled in Xenopus egg extract from purified DNA or chromatin resemble their natural counterparts in a number of structural and functional features. However, the most obvious structural element of normal interphase nuclei, the nucleolus, is absent from the in vitro reconstituted nuclei. By EM, cytological silver staining, and immunofluorescence microscopy employing antibodies directed against various nucleolar components we show that nuclei assembled in vitro contain numerous distinct aggregates that resemble prenucleolar bodies (PNBs) by several criteria. Formation of these PNB-like structures requires pore complex-mediated nuclear transport of proteins but is independent of the genetic content of the in vitro nuclei as well as transcriptional and translational events. Our data indicate that nuclei assembled in vitro are capable of initiating early steps of nucleologenesis but that the resulting PNBs are unable to fuse with each other, probably due to the absence of a functional nucleolus organizer. With appropriate modifications, this experimental system should be useful to define and analyze conditions promoting the site-specific assembly of PNBs into a coherent nucleolar body.

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Maller, James L. "Pioneering the Xenopus Oocyte and Egg Extract System: FIGURE 1." Journal of Biological Chemistry 287, no. 26 (2012): 21640–53. http://dx.doi.org/10.1074/jbc.x112.371161.

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Bauer, D. W., C. Murphy, Z. Wu, C. H. Wu, and J. G. Gall. "In vitro assembly of coiled bodies in Xenopus egg extract." Molecular Biology of the Cell 5, no. 6 (1994): 633–44. http://dx.doi.org/10.1091/mbc.5.6.633.

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When demembranated sperm nuclei are placed in a Xenopus egg extract, they become surrounded by a nuclear envelope and then swell to form morphologically typical pronuclei. Granules ranging from < 1.0 to approximately 3.0 microns in diameter appear within such nuclei. Bell et al. identified four nucleolar proteins in these "prenucleolar bodies" by immunofluorescent staining (fibrillarin, nucleolin, B23/NO38, 180-kDa nucleolar protein). By in situ hybridization we show that these bodies also contain U3 and U8 small nuclear RNAs (snRNAs), known to be involved in pre-rRNA processing. Moreover, they contain all the snRNAs involved in pre-mRNA splicing (U1, U2, U4, U5, and U6), as well as U7, which is required for histone pre-mRNA 3' end formation. In addition to the nucleolar antigens previously identified, we demonstrated staining with antibodies against the Sm epitope, trimethylguanosine, and coilin. Because the composition of these prenucleolar bodies is closer to that of coiled bodies than to nucleoli, we propose that they be referred to as coiled bodies. The existence of large coiled bodies in transcriptionally inactive pronuclei suggests that they may play a role in the import, assembly, and storage of RNA processing components but are not themselves sites of processing. In transcriptionally active nuclei coiled bodies could serve as sites for initial preassembly and distribution of snRNP complexes for the three major RNA processing pathways: pre-mRNA splicing, pre-rRNA processing, and histone pre-mRNA 3' end formation.

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Dimitrova, D. S., and D. M. Gilbert. "Regulation of mammalian replication origin usage in Xenopus egg extract." Journal of Cell Science 111, no. 19 (1998): 2989–98. http://dx.doi.org/10.1242/jcs.111.19.2989.

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Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese hamster ovary (CHO) cell nuclei are introduced into Xenopus egg extracts. At concentrations of intact late-G1-phase nuclei that approximate early Xenopus embryos, the entire genome was duplicated nearly 4 times faster than in culture, accompanied by a de-localization of initiation sites at the dihydrofolate reductase (DHFR) locus. As the concentration of nuclei was increased, the number of initiation sites per nucleus decreased and initiation at the DHFR locus became localized to the physiologically utilized DHFR origin. Origin specification was optimal at nuclear concentrations that approximate the Xenopus mid-blastula transition (MBT). Higher concentrations resulted in an overall inhibition of DNA synthesis. By contrast, with intact early G1-phase nuclei, replication initiated at apparently random sites at all concentrations, despite an identical relationship between nucleo-cytosolic ratio and replicon size. Furthermore, permeabilization of late-G1-phase nuclei, using newly defined conditions that preserve the overall rate of replication, eliminated site-specificity, even at nuclear concentrations optimal for DHFR origin recognition. These data show that both nucleo-cytosolic ratio and nuclear structure play important but independent roles in the regulation of replication origin usage. Nucleo-cytosolic ratio clearly influences the number of replication origins selected. However, titration of cytosolic factors is not sufficient to focus initiation to specific sites. An independent mechanism, effecting changes within G1-phase nuclei, dictates which of many potential initiation sites will function as an origin.

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Lestari, Liana Indah, Agustina Widyasworo, and Nita opi Ari kustanti. "PENGARUH PENAMBAHAN EKSTRAK KAYU SECANG (Caesalpinia sappan L.) DAN MINYAK IKAN LEMURU SEBAGAI ADITIF PAKAN TERHADAP PERFORMA TELUR DAN PRODUKTIVITAS PUYUH." AVES: Jurnal Ilmu Peternakan 12, no. 2 (2018): 10–21. http://dx.doi.org/10.35457/aves.v12i2.1505.

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This research aimed is to determine the effect of Secang extract addition and sardinella fish oil as feed additive on eggs performance and production of quail. The 98 quails and 10 weeks divided into 4 groups with unequal replication using the completely randomized design. The treatment which are P0 (based ration), P1 (based ration + 0,2% secang extract + 4% sardinella fish oil), P2 (based ration + 0,4% secang extract + 4% sardinella fish oil), P3 (based ration + 0,6% secang extract + 4% sardinella fish oil). The parameters observed were length storage time of egg, egg cholesterol rate, feed consumption, egg weight, hen day production, egg mass, feed conversion and mortality. Data were analyzed by ANOVA and Duncan’s analysis. The results showed that Secang extract addition and sardinella fish oil were increase egg cholesterol (P<0.05) and not significantly (P>0.05) on length storage time of egg and production of quail.

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Safaa, Abd El Wahab A. E., A. M. Abdul Azeem, Mansour A. Amal, and Al Shammari M. Eida. "Comparative biochemical studies of aqueous extract of Egyptian black and raw garlic (Allium sativum) on hypercholesterolemia in rats fed a high-fat diet." International Research Journal of Medicine and Medical Sciences 8, no. 4 (2020): 132–38. http://dx.doi.org/10.30918/irjmms.84.20.052.

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Black garlic is a type of garlic that is processed for a specific period, with precisely controlled degrees of moisture and heat until the color of raw garlic turns black. Black garlic plays a strong role in preventing diseases such as hyperlipidemia. This study aims to compare the effect of Egyptian black garlic extract (EBG) and Egyptian raw garlic extract (ERG) at different concentrations on serum biochemical parameters in hyperlipidemia rats’ model. Fifty albino rats were divided randomly into 10 groups with five animals. Rats were fed a normal diet (control) G1, high-fat diet (HFD) 40% lard w/w were (G2- G10) for four weeks to promoting hypercholesterolemia for all rats except G1 (control). After that, HFD groups were treated with 1ml oral administration three times a week of 100% (G3), 75% (G4), 50% (G5), and 25% (G6) EBG extract for 6 weeks. G2 (HFD) was a high-fat diet alone without any extract. ERG extract feed group were treated in the same manner described above, and G7 (HFD+100% ERG extract), G8 (HFD+75% ERG extract), G9 (HFD+50% ERG extract), and G10 (HFD+25% ERG extract) as written here. EBG extract-treated group showed significantly increased high-density lipoprotein cholesterol (HDL-C) in serum (p < 0.05), decreased both level of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG) in serum. EBG extract with high concentration (G3) causes down regulation of lipid and cholesterol (p < 0.05) comparing with ERG extract indicating usefulness for treating hypercholesterolemia. Body weights on rats decrease by feeding of EBG extract, along with liver enzymes decrease in EBG fed rats. This indicates the efficiency of black garlic extract in reducing the level of hypercholesterolemia and avoiding heart disease and atherosclerosis. Keywords: Allium sativum, garlic, black garlic, hypercholesterolemia, biochemical parameters.

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Dar, Muhammad Najam, Muhammad Usman Akram, Sajid Gul Khawaja, and Amit N. Pujari. "CNN and LSTM-Based Emotion Charting Using Physiological Signals." Sensors 20, no. 16 (2020): 4551. http://dx.doi.org/10.3390/s20164551.

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Novel trends in affective computing are based on reliable sources of physiological signals such as Electroencephalogram (EEG), Electrocardiogram (ECG), and Galvanic Skin Response (GSR). The use of these signals provides challenges of performance improvement within a broader set of emotion classes in a less constrained real-world environment. To overcome these challenges, we propose a computational framework of 2D Convolutional Neural Network (CNN) architecture for the arrangement of 14 channels of EEG, and a combination of Long Short-Term Memory (LSTM) and 1D-CNN architecture for ECG and GSR. Our approach is subject-independent and incorporates two publicly available datasets of DREAMER and AMIGOS with low-cost, wearable sensors to extract physiological signals suitable for real-world environments. The results outperform state-of-the-art approaches for classification into four classes, namely High Valence—High Arousal, High Valence—Low Arousal, Low Valence—High Arousal, and Low Valence—Low Arousal. Emotion elicitation average accuracy of 98.73% is achieved with ECG right-channel modality, 76.65% with EEG modality, and 63.67% with GSR modality for AMIGOS. The overall highest accuracy of 99.0% for the AMIGOS dataset and 90.8% for the DREAMER dataset is achieved with multi-modal fusion. A strong correlation between spectral- and hidden-layer feature analysis with classification performance suggests the efficacy of the proposed method for significant feature extraction and higher emotion elicitation performance to a broader context for less constrained environments.

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Fortea Gil, Carlos, and Belén Santana López. "Ensanchando los límites: Una aproximación desde la didáctica a la traducción literaria / Enlarging the limits: A didactic approach to literary translation." Education in the Knowledge Society (EKS) 13, no. 1 (2012): 90–110. http://dx.doi.org/10.14201/eks.8791.

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La problemática fundamental de la traducción literaria ronda siempre en torno a su propia posibilidad, los límites y metas de la reflexión sobre ella y la capacidad de trasladar esa reflexión a la práctica. En este contexto, ha sido metodológicamente frecuente partir del texto meta, tratando de extraer premisas, métodos y procedimientos. Nuestro planteamiento inicial pretende ser el inverso: partir de la traducción misma, en su vertiente aplicada a la didáctica, para extraer del proceso traductor tutelado posibles conclusiones sobre la esencia del proceso y su generalización para la práctica. La finalidad sería extraer principios teóricos a partir de principios prácticos, reflexiones al hilo de dificultades recurrentes, apuntes sobre posibles marcas textuales que apuntan a formatos de resolución de problemas (calidad de los textos originales, concepto de fidelidad, tono, etc., entendidos siempre desde el punto de vista de lo verificable o señalable en concreto en los textos). El modesto objetivo de este artículo es llamar la atención con ejemplos sobre la peculiaridad de la didáctica de la traducción literaria como posible método de indagación en la traducción literaria misma. The basic problem of literary translation lies in its very possibilities, in the limits and aims of all reflection on it, and in the capacity to shift this reflection to practice. In this context, the methodological starting point is normally the target text, from which premises, methods and procedures are extracted. In this paper, however, our aim is to take translation itself, as applied to didactics, as our starting point in order to extract from the supervised translating process possible conclusions regarding the essence of the process and its generalization for practice. The final aim is to extract theoretical principles based on practical principles, reflections on recurring difficulties, information about possible textual markers pointing to problem-solving formats (source text quality, loyalty concept, tone, etc., which are always understood from the point of view of what can be verified or pointed out specifically in texts). This paper aims, through examples, to draw attention to the peculiarity of literary translation didactics as a possible research method into literary translation.

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Lu, Z. H., D. B. Sittman, D. T. Brown, R. Munshi, and G. H. Leno. "Histone H1 modulates DNA replication through multiple pathways in Xenopus egg extract." Journal of Cell Science 110, no. 21 (1997): 2745–58. http://dx.doi.org/10.1242/jcs.110.21.2745.

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We investigated the effects of histone H1s on DNA replication using Xenopus egg extract. Mouse variants H1c and H10 were assembled onto Xenopus sperm chromatin by the extract during the remodeling that accompanies nuclear decondensation. The association of H1 with chromatin was rapid and concentration dependent. H1-associated chromatin displayed a typical nucleosomal repeat pattern indicating that linker histones are properly positioned along the DNA. The presence of H1 on sperm chromatin reduced both the rate and extent of DNA replication in egg extract. This reduction in rate is due, in part, to a delay in initiation of replication within individual nuclei. Initiation in extract is dependent upon nuclear assembly. Analysis of the assembly process revealed that H1 does not inhibit nuclear membrane formation or the import of nuclear protein, however, it does slow the rate of nuclear lamina formation. This H1-induced delay in lamina assembly is responsible for the delay in initiation as pre-assembled H1-containing nuclei initiate replication at the same time as control nuclei. However, H1 inhibits replication even when lamina assembly is complete suggesting that H1 also affects replication directly. These data indicate that H1 modulates DNA replication through multiple pathways in egg extract.

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Khoirunnisa, Itrah, Suherni Susilowati, Lilik Maslachah, Tri Wahyu Suprayogi, Rochmah Kurnijasanti, and Hermin Ratnani. "PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN." Ovozoa : Journal of Animal Reproduction 8, no. 2 (2020): 127. http://dx.doi.org/10.20473/ovz.v8i2.2019.127-131.

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The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.

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Basir, Buana, and Kaharuddin Kaharuddin. "Effectiveness of Ketapang Leaf Extract (Terminal Capta L) in Water Media on Crude Egg Hatching." International Journal on Advanced Science, Education, and Religion 3, no. 1 (2020): 33–39. http://dx.doi.org/10.33648/ijoaser.v3i1.44.

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Ketapang leaves are one of the leaves that are already known bioactive materials contained therein which can be utilized in aquaculture media. This study aims to analyze the use of ketapang (Termianal capta L.) leaf extract in water media on the hatchability of cork fish eggs (Channa striata). The research is expected to provide knowledge about the benefits of ketapang leaves in aquaculture media and the hatchability of cork fish eggs. The study was conducted at the Fish Seed Center (BBI) Bantimurung, Maros Regency. The test sample came from BBI Bantimurung. The study used 4 experimental methods with 3 replications each addition of ketapang leaf extract on water media, namely media without ketapang leaf extract (A), the addition of ketapang leaf extract 10 ppm (B), the addition of ketapang leaf extract 20 ppm (C), addition of 30 ppm ketapang leaf extract (D). The results showed that the treatment significantly affected the hatchability of cork fish eggs. The highest average hatchability of eggs in treatment D with a concentration of ketapang leaf extract was 30 ppm. Water quality parameters under optimal conditions, namely temperature 27.5-28 0C, pH 7.1-7.5, DO 4.5-5.5 ppm, NH3 0.001-0.016 ppm. Ketapang leaf extract can increase the hatchability of cork fish eggs to a concentration of 30 ppm.

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Lestari, Liana Indah, Anang Widigdyo, and Nita Opi Arikustanti. "PENGARUH PENAMBAHAN EKSTRAK KAYU SECANG (Caesalpinia sappan L.) DAN MINYAK IKAN LEMURU SEBAGAI ADITIF PAKAN TERHADAP DAYA SIMPAN TELUR DAN KOLESTEROL TELUR PUYUH." AVES: Jurnal Ilmu Peternakan 11, no. 2 (2018): 4. http://dx.doi.org/10.35457/aves.v11i2.277.

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This research aimed is to determine the effect of Secang extract addition and sardinella fish oil as feed additive on eggs performance and production of quail. The 98 quails and 10 weeks divided into 4 groups with unequal replication using the completely randomized design. The treatment which are P0 (based ration), P1 (based ration + 0,2% secang extract + 4% sardinella fish oil), P2 (based ration + 0,4% secang extract + 4% sardinella fish oil), P3 (based ration + 0,6% secang extract + 4% sardinella fish oil). The parameters observed were length storage time of egg, egg cholesterol rate. Data were analyzed by ANOVA and Duncan ’s analysis. The results showed that Secang extract addition and sardinella fish oil were increase egg cholesterol (P<0.05) and not significantly (P>0.05) on length storage time of egg and production of quail. Keywords: Caesalpinia sappan L., egg cholesterol, Sardinella fish oil.

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Lestari, Liana Indah, Anang Widigdyo, and Nita Opi Arikustanti. "PENGARUH PENAMBAHAN EKSTRAK KAYU SECANG (Caesalpinia sappan L.) DAN MINYAK IKAN LEMURU SEBAGAI ADITIF PAKAN TERHADAP DAYA SIMPAN TELUR DAN KOLESTEROL TELUR PUYUH." AVES: Jurnal Ilmu Peternakan 11, no. 2 (2018): 4. http://dx.doi.org/10.30957/aves.v11i2.277.

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This research aimed is to determine the effect of Secang extract addition and sardinella fish oil as feed additive on eggs performance and production of quail. The 98 quails and 10 weeks divided into 4 groups with unequal replication using the completely randomized design. The treatment which are P0 (based ration), P1 (based ration + 0,2% secang extract + 4% sardinella fish oil), P2 (based ration + 0,4% secang extract + 4% sardinella fish oil), P3 (based ration + 0,6% secang extract + 4% sardinella fish oil). The parameters observed were length storage time of egg, egg cholesterol rate. Data were analyzed by ANOVA and Duncan’s analysis. The results showed that Secang extract addition and sardinella fish oil were increase egg cholesterol (P<0.05) and not significantly (P>0.05) on length storage time of egg and production of quail. Keywords: Caesalpinia sappan L., egg cholesterol, Sardinella fish oil.

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Wafa, Nouioua, and Gaamoune Sofiane. "Antioxidant, anti-inflammatory and antimicrobial activities of methanolic and aqueous extract of the leaves of Pistacia lentiscus L." Journal of Animal & Plant Sciences 43.2 (February 29, 2020): 7407–14. http://dx.doi.org/10.35759/janmplsci.v43-2.1.

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1 ABSTRACT In this study, The Montaigne of Boutaleb contains a plant heritage characterized by the presence of several species that possess medicinal properties. The methanolic and aqueous extract of the leaves of Pistacia lentiscus were prepared, the yield of the methanolic extract was 2 .99 g the percentages 37.2% and the yield of the aqueous extract is 0.98 g with percentages 19.6%. The total dosage of polyphenols and flavonoids in the methanol extract was 38.27 ± 6.30 mg EAG/g extract and 9.80 ± 0,160, mg EAG/g extract, respectively. The total dosage of polyphenols and flavonoids in the aqueous extract was 26.76± 2.47 mg EAG/g extract and 4, 15±0.09 mg EAG/g extract, respectively. The effect of aqueous and methanolic extracts of mastic leaves were evaluated using the DPPH test. Both extracts have shown a weak trapping effect towards the free radicals with an IC50 68.27 ± 9.96 μg/ml and with an IC50 72.92 ± 9.43 μg/ml respectively, then weak activity antioxidant .The antibacterial activity of the methanolic and aqueous extract of the leaves of Pistacia lentiscus is significant. The aqueous and methanolic extract of mastic leaves possess a very strong anti-inflammatory activity to protect the membrane of human blood red blood cells.

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Qiao, Xiao Yan, and Chun Hui Wang. "The Identification of EEG Feature Evoked by Imaginary Movement." Applied Mechanics and Materials 427-429 (September 2013): 2059–63. http://dx.doi.org/10.4028/www.scientific.net/amm.427-429.2059.

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Aiming to the ERD/ERS phenomenon of left-right hand imaginary movement, this paper presents a method of wavelet transform combined with statistical analysis to extract EEG features evoked by imaginary movement. And the features were classified using the support vector machine based on RBF kernel and cross-validation accuracy (CVA) method. The results have shown that this method can perform effectively to extract features and reflect ERS and ERD characteristics of EEG signal. The accuracy of classification can reach 90% within the time costing 3.5 seconds. The highest signal to noise ratio is 1.445, and the maximum mutual information is 0.645bit. The results can meet the real-time brain-computer interface system.

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Zhou, Jing, Li Jun Li, Ning Shan Li, Xiao Ming Wu, and Rong Qian Yang. "Study on Identification Algorithm of EEG Imaginary Movements." Applied Mechanics and Materials 182-183 (June 2012): 1885–89. http://dx.doi.org/10.4028/www.scientific.net/amm.182-183.1885.

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Movement whether it is actual or imaginary can produce different electroencephalogram (EEG) signals. How to extract features of signals and accurately classify them is a key to brain-computer interface(BCI) system. In the paper, BCI competition data downloaded from BCI website are used as study object, through time-domain analysis and frequency-domain analysis, according to the attribute of event-related synchronization (ERS) and event-related desynchronization (ERD) during imagery movement, energy difference of lead C3 and C4 are selected as features and wavelet package is used to extract them. Probabilistic neural networks (PNN) is used as classification method. Compared with other two calssification methods such as support vector method (SVM) and liner classifier, the classification accuracy rate of PNN reaches to 89.2% steadily and is higher than them. It is proved that the method provided in the paper are effective for identifying imaginary movements.

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Juozapavičius, Algimantas, Gytis Bacevičius, Dmitrijus Bugelskis, and Rūta Samaitienė. "EEG analysis – automatic spike detection." Nonlinear Analysis: Modelling and Control 16, no. 4 (2012): 375–86. http://dx.doi.org/10.15388/na.16.4.14083.

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In the diagnosis and treatment of epilepsy, an electroencephalography (EEG) is one of the main tools. However visual inspection of EEG is very time consuming. Automatic extraction of important EEG features saves not only a lot of time for neurologist, but also enables a whole new level for EEG analysis, by using data mining methods. In this work we present and analyse methods to extract some of these features of EEG – drowsiness score and centrotemporal spikes. For spike detection, a method based on morphological filters is used. Also a database design is proposed in order to allow easy EEG analysis and provide data accessibility for data mining algorithms developed in the future.

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Novita, Cut Intan, Faqihuddin Nasution, and Eka Meutia Sari. "The The Efectiveness of Jamblang (Syzygium cumini) Leaves Extract Inclusion Skim Milk-Egg Yolk Extender on Motility and Viability of Aceh Cattle Spermatozoa during Pre-Freezing and Post-Thawing." Agricultural Science 3, no. 3 (2021): p12. http://dx.doi.org/10.30560/as.v3n3p12.

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The process of semen freezing causes an increase in free radicals concentration which can damage spermatozoa. The addition of natural ingredients in semen diluent is expected to solve this challenges. One of the natural ingredients that can be used is jamblang (Syzygium cumini) leaves. The objective of the current study was to investigate the quality of spermatozoa in Aceh cattle which was added with jamblang leaves extract in skim milk- egg yolk extender during pre-freezing and post-thawing. This study applied Completely Randomized Design (CRD) with 5 treatments and 5 replications. The treatments consisted of J0 = skim milk-egg yolk; J1 = skim milk-egg yolk + jamblang leaves extract 0.2%; J2 = skim milk-egg yolk + jamblang leaves extract 0.4%; J3 = skim milk-egg yolk + jamblang leaves extract 0.6%; and J4 = skim milk-egg yolk + jamblang leaves extract 0.8%. The parameters observed in this study were the percentage of motility and viability of frozen semen of Aceh cattle. The data obtained were analyzed using Analysis of Variance (ANOVA) and if differences were found, then it would be continued with Duncan's Multiple Distance test. The results showed that the addition of jamblang leaves extract in egg yolk skim milk significantly affected the percentage of motility during pre-freezing and post-thawing, significantly affected spermatozoa viability during pre-freezing and significantly affected the spermatozoa viability during post-thawing. J3 treatment (jamblang leaves extract 0.6 gram/100 ml) it should be higher than the other treatment, where the percentage of motility at pre-freezing and post-thawing were 55.48% and 52.71%, respectively, and the percentage of viability during pre-freezing and post-thawing were 56.59% and 53.94%, respectively. It was concluded that the addition of jamblang leaves extract in the skim milk-egg yolk extender affected the percentage of spermatozoa motility and viability of Aceh cattle during pre-freezing and post-thawing.

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Xu, Cheng Zhe, and Yong Gao Jin. "Human Identification Using One Lead ECG Signal." Advanced Materials Research 711 (June 2013): 670–73. http://dx.doi.org/10.4028/www.scientific.net/amr.711.670.

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This paper presents a new method for human identification using one lead ECG signal. First, preprocessing technique is used to perform the denoising. Then, QRS detection, modified HOAC, and DCT are utilized to extract features from preprocessed data. Finally, 1-nn classifier is used to classify the extracted features. The experimental result based on the proposed method shows the good performance in human identification.

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Swari, Wahyu Retno, Emy Koestanti Sabdoningrum, Wurlina Wurlina, Suherni Susilowati, Rochmah Kurnijasanti, and Erma Safitri. "PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN." Ovozoa : Journal of Animal Reproduction 8, no. 2 (2020): 122. http://dx.doi.org/10.20473/ovz.v8i2.2019.122-126.

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The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.

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Liur, Isye Jean. "Pengaruh Konsentrasi Ekstrak Daun Sirsak Terhadap Penurunan Kualitas Telur Ayam Ras Selama Penyimpanan." Jurnal Mutu Pangan : Indonesian Journal of Food Quality 7, no. 2 (2020): 85–89. http://dx.doi.org/10.29244/jmpi.2020.7.2.85.

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Hen egg is easy to get and affordable. Besides having a complete nutritional content, chicken eggs are also easily damaged due to microbial activity during storage so that it can reduce egg quality. Therefore, a preservation process is needed to maintain egg quality. This study aims to determine the effect of soursop leaf extract concentrations on decreasing the quality of eggs. This research used a completely randomized design with two factors, the first factor was the concentration of soursop leaf extract (20, 30, 40%) and the second was the shelf life (0, 7, 14, 21 and 28 days). Observation included egg white index, egg yolk index, haugh unit, and weights of eggs. The results indicate that eggs with soursop leaf extract immersion treatment can maintain egg quality. The concentration of soursop leaf extract as much as 40% gives better results in maintaining the quality of eggs.

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Oliveira, Helder Freitas de, Nadja Susana Mogyca Leandro, Alessandra Gimenez Mascarenhas, et al. "Egg characteristics of Japanese quail fed diets containing guava extract (Psidium guajava L.)." Acta Scientiarum. Animal Sciences 43 (April 23, 2021): e51892. http://dx.doi.org/10.4025/actascianimsci.v43i1.51892.

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The main purpose of this study was to evaluate the characteristics of eggs produced by Japanese quails fed with guava extract. A total of 400 eggs collected from the quails fed with experimental diets, were distributed in a completely randomized design in factorial arrangement 4 x 4 with main effects including four dietary levels of guava extract (0, 3, 6 and 9 g kg-1) and four storage periods (0, 9, 18 and 27 days) totalizing 16 treatments with five replicates of five eggs. The eggs were produced by Japanese quails, fed with experimental diets. The diets were isonutritive formulated by corn-soybean basis. The eggs were stored for different periods, at the same conditions, to constitute the treatments. The parameters evaluated were egg weight loss (g and %), yolk color, Haugh unit, specific gravity, eggshell thickness and pH of the yolk and albumen. Data were submitted to ANOVA, and means were compared using Scott-Knott test, using α = 0.05. The specific gravity of fresh eggs was better with the use of 6 g kg-1 of guava extract in the diet. The use of 3 g kg-1 of guava extract resulted in smaller eggshell thickness of fresh eggs. The Haugh unit was affected just by the storage periods. The dietary guava extract (3, 6 or 9 g kg-1) increased the pH of albumen in eggs stored for 27 days. The use of 6 g kg-1 of guava extract in quails diet increase the specific gravity in fresh eggs and increase the yolk color when the eggs are stored.

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Aam Gunawan, Muhammad Irwan Zakir, Siti Dharmawati, and Muhammad Rasyidi. "Influence of Dayak Onion Extract Concentration (Eleutherine palmi folia (L.) Merr) and Length Storage on Weight Decrease, Haugh Unit, and Albumen pH of Duck Egg." Indonesian Journal of Agricultural Research 2, no. 3 (2020): 147–55. http://dx.doi.org/10.32734/injar.v2i3.2050.

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The purpose of this study was to study the effect of immersion on dayak onion extract and storage time and the interaction of these two factors on decreasing of egg weight, haugh unit value and albumen pH of duck egg. The study used a completely randomized design of factorial pattern 4 x 4 with 4 replications. The treatment is as follows: The first factor is the concentration of onion dayak extract (A), consisting of: A0 = Eggs storage placed at room temperature; A1 = 10% concentrated of dayak onion extract; A2 = 20% concentrated of dayak onion extract; A3 = 30% concentrated of dayak onion extract; The second factor is the length of storage (B) consists of: B0 = 7 days. B1 = 14 days. B2 = 21 days. And B3 = 28 days. Research data were analyzed using analysis of variance and continued by Tukey Test. The results showed that the concentration of dayak onion extract did not affect the reduction in egg weight, but did affect the haugh unit and albumen pH. Egg weight reduction ranged from 0.92-1.02. Haugh unit and pH of albumen at 10% and 30% concentration of dayak onion extract did not differ, while the 0% concentration differed from all other treatments. Interaction effects between concentration of dayak onion extract and length storage were also significant for egg weight loss, Haugh unit, and pH of albumen. It is recommended that for preserving duck eggs, it is better to use a concentration of 30% onion extract.

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