Thematische Bibliographien / EPS-producing Bacteria

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  2. Dissertationen
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Auswahl der wissenschaftlichen Literatur zum Thema „EPS-producing Bacteria“

Autor: Grafiati
Veröffentlicht am 18. Mai 2024

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Zeitschriftenartikel zum Thema "EPS-producing Bacteria"

1

Zennathara, Sadia Afrin, Mohammad Ali, Mohammad Nurul Islam und Mihir Lal Saha. „Exopolysaccharide (EPS) Producing Bacteria of Sundarbans Mangrove Forest Soil“. Plant Tissue Culture and Biotechnology 32, Nr. 2 (29.12.2022): 145–56. http://dx.doi.org/10.3329/ptcb.v32i2.63549.

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The present study was undertaken to investigate the exopolysaccharide (EPS) producing bacteria from Sundarbans Mangrove Forest (SMF) soil of Bangladesh. The pH and aerobic heterotrophic bacterial counts of the soil samples ranged from 5.83 to 7.77 and 0.87 × 107 to 7.2 × 107 cfu/g, respectively. Potential 18 EPS producing bacterial isolates were selected for detailed study among which 15 were provisionally identified as members of the genus Bacillus, 2 were recognized as Dinococcus sp. and another one as Micrococcus sp. The genus Bacillus includes 7 distinct species viz. B. stearothermophilus, B. subtilis, B. brevis, B. marinus, B. schlegelli, B. pumilus and B. globisporous. The highest EPS production was found in the LB medium. The pHs of 6.5-7.5, 0% salinity, and 37°C temperature were found to be optimum for better growth of EPS producing bacteria. The three EPS producing bacterial isolates i.e. B. pumilus, B. globisporus and B. strearothermophilus were further confirmed through 16S rDNA sequence analysis. The culture and sensitivity (C/S) test results revealed streptomycin (S 10) as the most effective antibiotic to control the tested bacterial isolates. Plant Tissue Cult. & Biotech. 32(2): 145-156, 2022 (December)
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2

Zhang, Shuang, und Lan Wei Zhang. „Effect of Exopolysaccharide Producing Lactic Acid Bacterial on the Gelation and Texture Properties of Yogurt“. Advanced Materials Research 430-432 (Januar 2012): 890–93. http://dx.doi.org/10.4028/www.scientific.net/amr.430-432.890.

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Lactic acid bacterial play a important role in yogurt texture and gel quality. The performance of lactic acid bacteria starter directly affected the quality of yogurt. Exopolysaccharide (EPS)-producing LAB may improve the texture of fermented milks, depending on the strain. EPS production was found to have a major effect on the texture properties and gelation properties, but varying textures with EPS production, structure and interaction with milk proteins. Yoghurts fermented with EPS-producing cultures showed different mouth thickness and ropiness rheological parameters and varying syneresis and gel firmness. The mechanism that how the metabolic properties of EPS producing lactic acid bacteria affect the texture and gel quality of yogurt is reviewed in the article.
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Zennathara, Sadia Afrin, Mohammad Ali, Mohammad Nurul Islam und Mihir Lal Saha. „Exopolysaccharide producing bacteria of Sundarban Mangrove Forest soil and their antibiotic sensitivity profile“. Dhaka University Journal of Biological Sciences 32, Nr. 2 (20.07.2023): 243–55. http://dx.doi.org/10.3329/dujbs.v32i2.67683.

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This study focused on the investigation of Exopolysaccharide (EPS) producing bacteria from Sundarbans mangrove forest (SMF) soil, Bangladesh. The heterotrophic bacterial loads in the soil samples varied from 0.44×107 to 4.2×107 cfu/g indicating high bacterial load even under hostile environment. Fifteen EPS producing bacterial isolates were identified provisionally where thirteen isolates belonged to the genus Bacillus including B. badius (n=1, 6.67%), B. subtilis (n=3, 20.0%), B. pumilus (n=3, 20.0%), B. brevis (n=2, 13.33%), B. stearothermophilus (n=2, 13.33%), B. sphaericus (n=1, 6.67%) and B. alcalophilus (n=1, 6.67%). The remaining two isolates were recognized as the genus Micrococcus sp. (n=2, 13.33%). The genus Bacillus was predominant representing 86.67% abundance frequency. The LB medium was proven to be the most suitable medium for the growth of EPS producing bacterial isolates. 16S rDNA sequence analysis was conducted for three EPS producing bacterial isolates and they were identified as Bacillus subtilis, B. strearothermophilus and Micrococcus sp. The antibiogram profile of this study revealed streptomycin as the most effective antibiotic to control the growth of bacteria. The presence of antibiotic resistance bacteria in SMF soil is alarming for human health associated with this marine ecosystem. The multidrug resistance bacteria may come to the soil of SMF through the untreated discharged wastewaters and agricultural runoff from adjacent areas. Dhaka Univ. J. Biol. Sci. 32(2): 243-255, 2023 (July)
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Sree, Sudha. „A Study on Isolation and Identification of Exopolysaccharide (EPS) producing Bacteria from Soil“. International Journal for Research in Applied Science and Engineering Technology 9, Nr. 12 (31.12.2021): 650–71. http://dx.doi.org/10.22214/ijraset.2021.39341.

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Abstract: Polysaccharides are important potent molecules with their structural and compositional complexity which led to wide range of applications in various industries. The exopolysaccharides of microbial origin are released in response to extreme environmental conditions for the purpose of survival. The present study focuses on the isolation of exopolysaccharide producing bacteria from the soil sample and oil contaminated soil sample. Screening for the EPS production by the isolates is determined by the dry weight determination of precipitates of EPS and quantitative estimation of glucose content of EPS by PhenolSulphuric acid method. In the present study, out of 5 bacterial isolates isolated on screening, Lactobacillus sps and Pseudomonas sps. isolates produced the precipitates of EPS whose dry weight was determined to be 0.09g and 0.17g respectively. Further, glucose concentration of EPS was quantitatively determined. The glucose content of Lactobacillus sps. isolate was 0.1125mg/ml and Pseudomonas sps. isolate is 0.2875mg/ml. The EPS producing isolates were further grown in the presence of carbon sources like Glucose, Lactose, Maltose and Sucrose to determine the best utilizable carbon for their growth. The most utilizable carbon source for maximum growth of EPS producing isolates was determined to be sucrose with 2% concentration. All the 5 bacterial isolates were screened for their ability of antibiotic resistance. The EPS producing isolates, Lactobacillus sps, Pseudomonas sps were found to be resistant towards all the antimicrobial agents owing to the presence of EPS protective layer around their cell wall than non-EPS producing isolates. Keywords: Exopolysaccharide, Screening, Carbon sources, Antibiotic resistance.
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H Patel, Vishal, Harsha P Soni und Falguni R Patel. „Isolation, Screening and Molecular Characterization of Exopolysaccharide Producing Moderately Halotolerant Bacteria from Various Coastal Sites of Gujarat“. Biosciences Biotechnology Research Asia 19, Nr. 1 (31.03.2022): 231–42. http://dx.doi.org/10.13005/bbra/2981.

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The exopolysaccharides (EPSs) are natural polymers of carbohydrates and are excreted by some bacteria outside of their cell walls. The microbial EPS have several biotechnological applications viz. cosmetics, textiles, pharmaceuticals, agriculture, paints and petroleum industries. The wide range of applications and bioactive roles of EPS has triggered increased interest for search unusual and novel EPS.The bacteria from marine ecosystem are also known to secrete novel EPSs. In this context, the main objective of this research is isolation, screening of most potent culturable EPS producing halotolerant bacteria with novel EPS characteristics which can be used in uncommon applications related to the environment. All the bacterial isolates were isolated from coastal regions of Gujarat as it contains 1600 km long costal area, with wide microbial diversity and can serve as a source for promising EPS producers. 9 soil samples were collected from various coastal sites viz. Mundra, Jodiya, Dwarka, Somnath, Diu, Bhavnagar, Khambhat, Dumas and Umargam. Total 59 EPS producing bacterial isolates were obtained in Primary Screening. Based on the results of primary screening, potential morphologically diverse 9 isolates were selected for EPS production in liquid medium. The EPS production ranged from 22.3 to 33.5 mg/ml. The isolate VHP 34 gave best EPS production and was identified as Enterobacter cloacae by 16 s rRNA gene sequencing method. The isolate Enterobacter cloacae VHP-34 was able to grow 0-15% NaCl concentration, hence categorized as Moderately Halotolerant.
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Gunasekaran, Yazhini, Subramaniam Thiyageshwari, Manikandan Ariyan, Aritra Roy Choudhury, Jung-Ho Park, Duraisamy Selvi, Lakshmanan Chithra und Rangasamy Anandham. „Alleviation of Sodic Stress in Rice by Exploring the Exopolysaccharide-Producing Sodic-Tolerant Bacteria“. Agriculture 12, Nr. 9 (13.09.2022): 1451. http://dx.doi.org/10.3390/agriculture12091451.

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Sodicity is one of the major salt stresses that impair crop production. Exopolysaccharide-producing sodic tolerant bacteria (EPS-STB) play a significant role in reducing the sodic stress in plants by hampering the uptake of sodium. In this context, this study aims to isolate the EPS-STB for alleviating sodic stress in rice under a sodic environment. Thus, artificial sodicity was created in culture media, and 253 bacteria were isolated from the rice rhizosphere of sodic soils in Trichy and Chinna Salem of Tamil Nadu in India. Fifty bacterial isolates were initially screened based on EPS production, sodic tolerant ability, and plant growth-promoting activities. Further, these bacterial isolates were identified using 16S rDNA sequencing. The results suggested that the isolated bacteria possessed biofilm-forming abilities along with plant growth-promoting activities and osmolyte accumulation under sodic stress conditions. Bacillus rugosus L1C7T, Bacillus paralicheniformis L1C5L, Pseudomonas sp. L5C14T and Franconibacter helveticus L2C1L2 were chosen as better EPS-STB plant growth-promoting bacteria, and their impact on rice under sodic conditions was evaluated. Among the sodic tolerant bacteria, Franconibacter helveticus L2C1L2-inoculated rice plants increased dry matter production compared to the control. Thus, this study showed that the utilization of EPS-STB will become a promising tool to alleviate sodic stress in rice.
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Biswas, Jhuma, und A. K. Paul. „Production of Extracellular Polymeric Substances by Halophilic Bacteria of Solar Salterns“. Chinese Journal of Biology 2014 (06.08.2014): 1–12. http://dx.doi.org/10.1155/2014/205731.

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Moderately halophilic aerobic bacteria were isolated from 31 soil and 18 water samples collected from multipond solar salterns of Gujarat, Orissa, and West Bengal, India. A total of 587 bacterial isolates with distinct morphological features were obtained from these samples following dilution and plating on MH agar medium supplemented with NaCl. The isolates were screened for growth associated extracellular polymeric substances (EPS) production in MY medium under batch culture. In all, 20 isolates were selected as potent ones producing more than 1 g/L of EPS. These EPS producing isolates were characterized in detail for their morphological, physiological, and biochemical features and tentatively identified as members belonging to the genera Halomonas, Salinicoccus, Bacillus, Aidingimonas, Alteromonas, and Chromohalobacter. Apart from EPS production, these isolates also hold promise towards the production of various biomolecules of industrial importance.
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Provencher, Cathy, Gis�le LaPointe, St�phane Sirois, Marie-Rose Van Calsteren und Denis Roy. „Consensus-Degenerate Hybrid Oligonucleotide Primers for Amplification of Priming Glycosyltransferase Genes of the Exopolysaccharide Locus in Strains of the Lactobacillus casei Group“. Applied and Environmental Microbiology 69, Nr. 6 (Juni 2003): 3299–307. http://dx.doi.org/10.1128/aem.69.6.3299-3307.2003.

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ABSTRACT A primer design strategy named CODEHOP (consensus-degenerate hybrid oligonucleotide primer) for amplification of distantly related sequences was used to detect the priming glycosyltransferase (GT) gene in strains of the Lactobacillus casei group. Each hybrid primer consisted of a short 3′ degenerate core based on four highly conserved amino acids and a longer 5′ consensus clamp region based on six sequences of the priming GT gene products from exopolysaccharide (EPS)-producing bacteria. The hybrid primers were used to detect the priming GT gene of 44 commercial isolates and reference strains of Lactobacillus rhamnosus, L. casei, Lactobacillus zeae, and Streptococcus thermophilus. The priming GT gene was detected in the genome of both non-EPS-producing (EPS−) and EPS-producing (EPS+) strains of L. rhamnosus. The sequences of the cloned PCR products were similar to those of the priming GT gene of various gram-negative and gram-positive EPS+ bacteria. Specific primers designed from the L. rhamnosus RW-9595M GT gene were used to sequence the end of the priming GT gene in selected EPS+ strains of L. rhamnosus. Phylogenetic analysis revealed that Lactobacillus spp. form a distinctive group apart from other lactic acid bacteria for which GT genes have been characterized to date. Moreover, the sequences show a divergence existing among strains of L. rhamnosus with respect to the terminal region of the priming GT gene. Thus, the PCR approach with consensus-degenerate hybrid primers designed with CODEHOP is a practical approach for the detection of similar genes containing conserved motifs in different bacterial genomes.
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MENAGA, M., S. FELIX, C. MOHANASUNDARI und M. CHARULATHA. „Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon source“. Indian Journal of Animal Sciences 90, Nr. 5 (10.09.2020): 819–23. http://dx.doi.org/10.56093/ijans.v90i5.104640.

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The present study aimed to isolate and characterize the Extracellular polymeric substance (EPS) producing bacteria from biofloc reared Nile tilapia (Chitralada) ponds. Distillery spentwash was used as a carbon source to maintain the C: N ratio at 10: 1 in the fish culture ponds and screening of bacteria were done fortnightly in 180 days culture. Out of 38 bacterial isolates, 7 isolates were found to produce EPS. Based on 16s rRNA sequence analysis the isolates were identified as Bacillus subtilis, B. megaterium, B. infantis, B. cereus, Pseudomonas balearica, P. mendocina and P. alcaligenes. The highest production of EPS was recorded in B. cereus (1.25 g/L). EPS extracted from Bacillus cereus was reported to have higher protein (89 μg/ml) and B. subtilis possessed higher carbohydrate (753.75 μg/ml). Maximum flocculating ability of 40.18% in B. cereus and higher emulsifying activity of 63.53% was observed in B. megaterium. The EPS extracted from B. infantis showed lower sludge volume index on its treatment with aquaculture sludge (15.38 ml/g). Absorption band in the range of 4,000/cm to 450/cm using FTIR analysis confirmed the presence of characteristic functional bands arising from polysaccharides, nucleic acids and proteins. The results indicated the presence of EPS producing bacteria in biofloc based Nile tilapia aquaculture systems.
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Laraib, Fizza, Rahmeen Rauf, Rimsha Dilshad, Nazia Jameel und Rida Batool. „Characterization of Halophilic Bacteria Isolated from Khewra Salt Mines“. Lahore Garrison University Journal of Life Sciences 6, Nr. 02 (15.06.2022): 133–47. http://dx.doi.org/10.54692/lgujls.2022.0602214.

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Halophilic bacteria can populate every niche of earth. These halophiles have a great potential of exopolysaccharides production that is of considerable importance in various industries. To screen EPS producing halophiles, saline soil samples were collected from Khewra salt mines, Jehlum, Pakistan. Twenty-two morphologically different bacterial strains were isolated by serial dilution method. All strains were considered as moderate halophilic bacteria as they could grow at 3-15% of NaCl concentration whereas only three strains could grow at 15% of NaCl, which belonged to the genus Bacillus and Pseudomonas. For screening of EPS production, P-medium was used. While for the estimation of slime production, congo-red agar was used that exhibited positive results by appearance of black colored colonies by many strains. Moreover, EPS production was analyzed quantitatively and qualitatively. Isolated Staphylococcus and Bacillus species produced high amount of EPS (20g/L). “Moderate halophiles” play an important role in therapeutics, bioremediation, food and medicine, petroleum and tanning industries by producing EPS. Recently, growth of many agriculture crops has been improved by using beneficial halophiles in saline soils. Consequently, with the help of these beneficial halophiles we can give benefit to mankind
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Dissertationen zum Thema "EPS-producing Bacteria"

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Mandal, Amit Kumar. „Exploring physiology of an exopolysaccharide(EPS) producing facultatively oligotrophic bacterium klebsileea pneumoniae pb12 with special emphasis on structure -function analysis of EPS“. Thesis, University of North Bengal, 2015. http://ir.nbu.ac.in/hdl.handle.net/123456789/2755.

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Yang, Xuejiao. „Initial adhesion of EPS producing bacteria Burkholderia cepacia the impact of cranberry juice“. Master's thesis, 2010. http://hdl.handle.net/10048/1354.

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The impact of cranberry juice was investigated with respect to the initial adhesion of three isogenic Burkholderia cepacia bacteria with different extracellular polymeric substance (EPS) producing capacities: a wild-type cepacian EPS producer PC184, mutant bacteria PC184rml with reduced EPS production, and PC184bceK with a deficiency in EPS production. Adhesion experiments demonstrated that in the presence of cranberry juice, the adhesive capacity of PC184 was largely reduced, while cranberry juice had little impact on the adhesion of either mutant. Thermodynamic modeling supported results from adhesion experiments. For PC184, the surface free energy change Gadh switched from negative in the absence of cranberry juice to positive when cranberry juice was added. Surface force apparatus (SFA) and scanning electron microscopy (SEM) studies demonstrated strong adsorption of cranberry juice components to bacterial EPS. It was concluded that cranberry juice components could impact bacterial initial adhesion by adhering to EPS and impairing bacterial adhesive capacity.
Environmental Engineering
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Amatayakul, Thanut. „The improvement of physical properties of yoghurts by varying casein/whey protein ratio and EPS-producing starter cultures“. Thesis, 2005. https://vuir.vu.edu.au/15558/.

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The overall objective of this study was to investigate effects of variation in casein to whey protein ratios and types of exopolysaccharide producing starter cultures on physical properties of yoghurts made at 9% and 14% total solids during 28 days of storage at 4°C. There are three main parts of this research: a preliminary study, a comparative study of three methods for determination of syneresis and the studies on physical properties of yoghurts made with various casein to whey protein ratios using non-exopolysaccharide or exopolysaccharide-producing starter cultures.
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Buchteile zum Thema "EPS-producing Bacteria"

1

Vassoler Serrato, Rodrigo. „Bacterial Alginate Biosynthesis and Metabolism“. In Alginate - Applications and Future Perspectives [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.109295.

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Alginate is a linear anionic heteropolysaccharide with a chemical structure consisting of 1,4-linked subunits of β-D-mannuronic acid (M) and its C-5 epimer α-L-guluronic acid (G). It is well known that the monomer composition and molecular weight of alginates affect their properties and influence their use in the food and pharmaceutical industries. Alginate is usually extracted from seaweed for commercial purposes, but can also be produced by bacteria as exopolysaccharide (EPS). Pseudomonas spp. and Azotobacter vinelandii are well-known alginate-producing microorganisms. Their biochemical machinery for alginate biosynthesis is influenced by changing culture conditions and manipulating genes/proteins, making it relatively easy to obtain customized EPS with different molecular weights, M/G compositions, and thus physicochemical properties. Although these two genera have very similar biosynthetic pathways and molecular mechanisms for alginate production, with most of the genes involved being virtually identical, their regulation has been shown to be somewhat different. In this chapter, we present the main steps of alginate biosynthesis in bacteria, including precursor synthesis, polymerization, periplasmic modifications, transport/secretion, and post-secretion modification.
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Munagala, Shivani, Manikantha Dunna, C. D. S. L. N. Tulasi, Bansuwada Sowmya, Bussa Archana, Venkata Suresh Ponnuru und Kalyani Chepuri. „Integrating QQ with Nano-techniques – A Potent Antibacterial Therapy“. In Quorum Quenching, 368–92. Royal Society of Chemistry, 2023. http://dx.doi.org/10.1039/bk9781837671380-00368.

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Biofilms are highly organized 3D structures where bacteria are embedded in a self-produced complex matrix, made of extracellular polymeric substances (EPS). Biofilms are produced via the quorum sensing (QS) pathway. QS is mediated by bacteria producing, releasing and detecting extracellular signaling molecules called autoinducers (AIs). If AIs are produced more, then the biofilm’s cell density will be greater, these biofilms are responsible for microbial activity. As of now there are at least three known mechanisms for QS pathways. Those include (1) luminescence inducer/regulator (LuxI/LuxR)-type quorum sensing with acyl-homoserine lactones (AHL) as signal molecules (in Gram-negative bacteria); (2) oligopeptide two-component-type quorum sensing with short peptides as signal molecules (in Gram-positive bacteria); and (3) AI-2 quorum sensing: expressed via the luxS gene (in both types of bacteria). To produce antimicrobial activity, the QS mechanism needs to be suppressed. Quorum quenching (QQ), works against QS, which, in turn, helps in antimicrobial activity. In some cases, AHL is modified to disrupt QS mechanisms. For example, Agrobacterium and Pseudomonas, can cleave their own AHL signals. Recent research in nanotechnology has resulted in the development of nanoparticle drugs, which showed the anti-QS mechanisms. On the basis of their unique properties, nanoparticles act as anti-infective agents, so they are used in drug development. Nanoparticles (NPs) do not destroy cell wall to kill the microbes but they act at cellular level, that is the biochemical and molecular level, and destroy the membranes. Some of the nanoparticles used in drug development are silver NPs, gold NPs, ZnO NPs, SiO2 NPs and TiO2 NPs. Nanoparticles help in eliminating the QS, which leads to a low density biofilm (through a decrease in bacterial growth) which helps to treat and prevent bacterial infection.
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