Thematische Bibliographien / Dual lifetime referencing

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Auswahl der wissenschaftlichen Literatur zum Thema „Dual lifetime referencing“

Autor: Grafiati

Veröffentlicht am 22. Februar 2025

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Zeitschriftenartikel zum Thema "Dual lifetime referencing"

Huber, Christian, Ingo Klimant, Christian Krause und Otto S. Wolfbeis. „Dual Lifetime Referencing as Applied to a Chloride Optical Sensor“. Analytical Chemistry 73, Nr. 9 (Mai 2001): 2097–103. http://dx.doi.org/10.1021/ac9914364.

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Chen, Wan-Har, Evelyn Armstrong, Peter W. Dillingham, Stephen C. Moratti, Courtney Ennis und Christina M. McGraw. „Dual-Lifetime Referencing (t-DLR) Optical Fiber Fluorescent pH Sensor for Microenvironments“. Sensors 23, Nr. 21 (31.10.2023): 8865. http://dx.doi.org/10.3390/s23218865.

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The pH behavior in the μm to cm thick diffusion boundary layer (DBL) surrounding many aquatic species is dependent on light-controlled metabolic activities. This DBL microenvironment exhibits different pH behavior to bulk seawater, which can reduce the exposure of calcifying species to ocean acidification conditions. A low-cost time-domain dual-lifetime referencing (t-DLR) interrogation system and an optical fiber fluorescent pH sensor were developed for pH measurements in the DBL interface. The pH sensor utilized dual-layer sol-gel coatings of pH-sensitive iminocoumarin and pH-insensitive Ru(dpp)3-PAN. The sensor has a dynamic range of 7.41 (±0.20) to 9.42 ± 0.23 pH units (95% CI, T = 20 °C, S = 35), a response time (t90) of 29 to 100 s, and minimal salinity dependency. The pH sensor has a precision of approximately 0.02 pHT units, which meets the Global Ocean Acidification Observing Network (GOA-ON) “weather” measurement quality guideline. The suitability of the t-DLR optical fiber pH sensor was demonstrated through real-time measurements in the DBL of green seaweed Ulva sp. This research highlights the practicability of optical fiber pH sensors by demonstrating real-time pH measurements of metabolic-induced pH changes.

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Liebsch, Gregor, Ingo Klimant, Christian Krause und Otto S. Wolfbeis. „Fluorescent Imaging of pH with Optical Sensors Using Time Domain Dual Lifetime Referencing“. Analytical Chemistry 73, Nr. 17 (September 2001): 4354–63. http://dx.doi.org/10.1021/ac0100852.

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Borisov, Sergey M., Gerhard Neurauter, Claudia Schroeder, Ingo Klimant und Otto S. Wolfbeis. „Modified Dual Lifetime Referencing Method for Simultaneous Optical Determination and Sensing of Two Analytes“. Applied Spectroscopy 60, Nr. 10 (Oktober 2006): 1167–73. http://dx.doi.org/10.1366/000370206778664590.

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Begemann, Jens, und Antje C. Spiess. „Dual lifetime referencing enables pH-control for oxidoreductions in hydrogel-stabilized biphasic reaction systems“. Biotechnology Journal 10, Nr. 11 (September 2015): 1822–29. http://dx.doi.org/10.1002/biot.201500198.

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Maierhofer, Maximilian, Sergey M. Borisov und Torsten Mayr. „Optical Ammonia Sensor for Continuous Bioprocess Monitoring“. Proceedings 2, Nr. 13 (19.11.2018): 1041. http://dx.doi.org/10.3390/proceedings2131041.

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We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.

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Lin, Xuyan, Wenting Qiu, Gianmarco Domenico Suarez und Stefan Nagl. „Extracellular pH Monitoring of Live Single Cells in Microdroplets Using Dual-Labelled Fluorinated Silica Nanoparticles and Time-Domain Dual Lifetime Referencing“. Chemosensors 10, Nr. 10 (21.09.2022): 379. http://dx.doi.org/10.3390/chemosensors10100379.

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Fluorinated silica nanoparticles doped with Ruthenium-tris-1,10-phenanthroline dichloride on the inside and covalently conjugated with perfluorooctyltriethoxysilane and fluorescein isothiocyanate on the outside were developed and served several functions; the fluorination of the particles served to stabilize droplets in a microfluidic system at their interface to the continuous phase for single-cell experiments, and the two dyes provided for intrinsically referenced pH readout according to the time-domain dual lifetime referencing scheme. Apart from eliminating the droplet-to-droplet transport, these nanoparticles at the interface of the droplets generated rigid substrates that were suitable for the proliferation of adherent cells in the droplets without additional matrices. Cancer and non-cancer cell lines with culture media were allowed to proliferate in the droplets and the extracellular pH was monitored. These nanoparticles used in a microdroplet system could measure the pH of the extracellular microenvironment of single cells and provide support for the growth of cells in droplets of around 50 µm diameter. The pHe showed 6.84 ± 0.04 and 6.81 ± 0.04 for cancer cells (MCF-7 and A549, respectively) and 7.36 ± 0.03 for healthy cells (HUVEC), after a 10-h incubation, which can be potentially applied in distinguishing tumor from non-tumor cells. Capable of assisting cell culture and pH sensing in droplet microfluidic systems, the dye-conjugated fluorinated nanoparticles described in this work offer possibilities in a variety of biochemical or environmental analytical applications.

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Rowe, H. M., Sing Po Chan, J. N. Demas und B. A. DeGraff. „Self-Referencing Intensity Measurements Based on Square-Wave Gated Phase-Modulation Fluorimetry“. Applied Spectroscopy 57, Nr. 5 (Mai 2003): 532–37. http://dx.doi.org/10.1366/000370203321666551.

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An adaptation of square-wave gated phase-modulation (GPM) fluorimetry allows for self-referenced intensity measurements without the complexity of dual excitation or dual emission wavelengths. This AC technique utilizes square-wave excitation, gated detection, a reference emitter, and a sensor molecule. The theory and experimental data demonstrating the effectiveness and advantages of the adapted GPM scheme are presented. One component must have an extremely short lifetime relative to the other. Both components are affected identically by changes in intensity of the excitation source, but the sensor intensity also depends on the concentration of the analyte. The fluctuations of the excitation source and any optical transmission changes are eliminated by ratioing the sensor emission to the reference emission. As the concentration of the analyte changes, the corresponding sensor intensity changes can be quantified through several schemes including digitization of the signal and digital integration or AC methods. To measure pH, digital methods are used with Na3[Tb(dpa)3] (dpa = 2,6-pyridinedicarboxylic acid) as the long-lived reference molecule and fluorescein as the short-lived sensor molecule. Measurements from the adapted GPM scheme are directly compared to conventional ratiometric measurements. Good agreement between the data collection methods is demonstrated through the apparent pKa. For the adapted GPM measurements, conventional measurements, and a global fit the apparent pKa values agree within less than 2%. A key element of the adapted GPM method is its insensitivity to fluctuations in the source intensity. For a roughly 8-fold change in the excitation intensity, the signal ratio changes by less than 3%.

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Boniello, Caterina, Torsten Mayr, Juan M. Bolivar und Bernd Nidetzky. „Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts“. BMC Biotechnology 12, Nr. 1 (2012): 11. http://dx.doi.org/10.1186/1472-6750-12-11.

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Poehler, Elisabeth, Christin Herzog, Madeleine Suendermann, Simon A. Pfeiffer und Stefan Nagl. „Development of microscopic time-domain dual lifetime referencing luminescence detection for pH monitoring in microfluidic free-flow isoelectric focusing“. Engineering in Life Sciences 15, Nr. 3 (05.03.2015): 276–85. http://dx.doi.org/10.1002/elsc.201400081.

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Dissertationen zum Thema "Dual lifetime referencing"

Dervieux, Emmanuel. „Non-invasive transcutaneous characterisation of blood carbon dioxide (CO2) content“. Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAD043.

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Cette thèse cherche à développer une méthode non-invasive de mesure du taux de dioxyde de carbone (CO2) artériel, dont la connaissance est cruciale sur le plan clinique. Deux approches principales sont étudiées : la carbamétrie pulsée, qui extrapole les principes de l'oxymétrie à la carbamino-hémoglobine via des techniques spectrophotométriques, et l’étude de la diffusion transcutanée du CO2, qui donne des indications sur le contenu en CO2 des tissus sous-cutanés. La première approche s'est révélée être une impasse, tandis que la seconde montre un plus grand potentiel. En particulier, la piste d’un capteur se présentant sous la forme d’un patch contenant un fluorophore sensible au pH semble particulièrement prometteuse. Une première étude clinique visant à mieux caractériser la diffusion transcutanée du CO2 a été réalisée, permettant le dimensionnement d’un tel patch. Une étude théorique approfondie est ensuite menée, et des prototypes de patchs sont conçus, présentant une réponse au CO2 satisfaisante. La thèse conclut que – malgré les nombreuses améliorations qui restent à apporter au patch ainsi conçu – cette nouvelle modalité de mesure pourrait permettre le suivi médical continu et portatif du CO2
This thesis aims to develop a non-invasive method for measuring arterial carbon dioxide (CO2) content, which is crucial in clinical practice. Two main approaches are investigated: pulse carbametry, which extrapolates oximetry principles to carbaminohaemoglobin using spectrophotometric techniques, and the study of transcutaneous CO2 diffusion, which provides insights into the CO2 content of subcutaneous tissues. The first approach proved to be a dead-end, while the second shows greater potential. In particular, the concept of a sensor in the form of a patch containing a pH-sensitive fluorophore appears especially promising. A preliminary clinical study was conducted to better characterize transcutaneous CO2 diffusion, enabling the dimensioning of such a patch. An in-depth theoretical study followed, leading to the development of patch prototypes that exhibited a satisfactory response to CO2. This thesis concludes that, despite the many improvements still needed for the patch to be usable in a real-life clinical context, this new measurement modality could pave the way for continuous and portable CO2 monitoring in medical care

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Buchteile zum Thema "Dual lifetime referencing"

Klimant, I., C. Huber, G. Liebsch, G. Neurauter, A. Stangelmayer und O. S. Wolfbeis. „Dual Lifetime Referencing (DLR) — a New Scheme for Converting Fluorescence Intensity into a Frequency-Domain or Time-Domain Information“. In New Trends in Fluorescence Spectroscopy, 257–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-56853-4_13.

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Konferenzberichte zum Thema "Dual lifetime referencing"

Dervieux, Emmanuel, und Wilfried Uhring. „Dual Lifetime Referencing for Accurate CO₂ Sensing: An Experimental in vitro Validation“. In 2024 22nd IEEE Interregional NEWCAS Conference (NEWCAS), 253–57. IEEE, 2024. http://dx.doi.org/10.1109/newcas58973.2024.10666315.

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Tufan, Tuna B., und Ulkuhan Guler. „A Miniaturized Transcutaneous Carbon Dioxide Monitor Based on Dual Lifetime Referencing“. In 2022 IEEE Biomedical Circuits and Systems Conference (BioCAS). IEEE, 2022. http://dx.doi.org/10.1109/biocas54905.2022.9948600.

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