Auswahl der wissenschaftlichen Literatur zum Thema „Drinking water – Purification – Problems, exercises, etc“

Safe potable water for human use has become an emerging concern in this era. A Programmable Logic Controller (PLC) based automatic water treatment plant has been designed to remove unexpected materials from water and to confirm drinking quality of water. The conventional methods used in older times result into problems like empty running, dose and coagulation control, overflow, leakage etc. The operations of the plant such as water intake from raw water tank, water intake for chlorination, mixing of chlorine water with raw water, and water feed into various treatment chambers are done automatically. For automation PLC and microcontroller are used to control the above-mentioned functions. The iron content is reduced to negligible amount and contaminations are removed to ensure safe, odorless, clean water.

The life support system of the International Space Station must include the provision of drinking water to the crew and the treatment and disposal of wastewater. The cost of water delivery to the ISS is very high, so it is necessary to improve the technological schemes of wastewater treatment in space in order to reuse water in a complete closed cycle. The studies were performed based on the analysis of Ukrainian and foreign scientific sources and reporting data on the specifics of water use at space stations and the treatment methods of the used waters (wastewaters). In addition to international experience, our own research was used to develop a technology for wastewater treatment in space. The authors of the article analyzed the operation of existing wastewater treatment facilities in space and made recommendations for their use at the ISS. The developed technology for the treatment of wastewater and drinking water in zero-gravity (space) is based on the use of various reactors. They can be made of various materials (metal, plastic, etc.); they do not contain non-standard equipment that requires factory manufacturing. Compactness, complete tightness and small dimensions of bio- and physicochemical reactors allow them to be installed within the ISS. The cleaning process is easy to manage and can be fully automated. Water problems are central to the whole world, including in space. The ISS should have a system for the wastewater treatment and their closed use, since the supply of new water to stations significantly increases the cost of space exploration. Quality water is the health and well-being of people in space. Since there is no gravity in space, centrifugal forces (centrifuges) must be used to separate suspended particles from water. A comprehensive review of the issues related to wastewater treatment in space, allows us to conclude that it is necessary to regenerate water at International space stations (ISS). Indeed, to ensure the life support of the astronauts, a colossal amount of water is required, and its delivery to the ISS from the Earth is expensive.

Plenty of fresh water resources are still inaccessible for human use. Calamities such as pollution, climate change, and global warming pose serious threats to the fresh water system. Although many naturally and synthetically grown materials have been taken up to resolve these issues, there is still plenty of room for enhancements in technology and material perspectives to maximize resources and to minimize harm. Considering the challenges related to the purification of water, materials in the form of nanofiber membranes and nanomaterials have made tremendous contributions to water purification and filtration. Nanofiber membranes made of synthetic polymer nanofibers, ceramic membranes etc., metal oxides in various morphologies, and carbonaceous materials were explored in relation to waste removal from water. In this review, we have discussed a few key materials that have shown effectiveness in removing pollutants from waste water, enabling solutions to existing problems in obtaining clean drinking water.

Safe drinking water is one of the most important conditions for a healthy life. However, in case of disasters and emergencies, the water is often contaminated with various impurities of physical, chemical and/or biological origin. These contaminations can lead to a number of health problems, including various infectious diseases. For that reason, it is important to act preventively, and to perform appropriate treatment and water purification in a timely and urgent manner, depending on the type of pollution. In order to determine the type of pollution and perform the appropriate water treatment, the precondition is arranging certain chemical analyzes and monitoring of water quality through quality parameters. Since our time and economic resources are limited in the first moments of the accident, it is not possible to monitor all the parameters, so we monitor the most important: pH value, amount of residual chlorine, color, turbidity and the presence of pathogens. However, even when the type of pollution is determined, it is sometimes not possible to do centralized water purification immediately. Therefore, it is important to know the methods that can independently, and with the help of some handy tools, be applied in our household (eg. disinfection by boiling water or using some of the chemicals for disinfection; sedimentation, etc.). Using these methods, at least a physiological minimum can be provided for family members in the first moments after the accident, until a centralized purification is performed.

In case of cleaning the tap water in Korea, focus has been concentrated on how to improve water quality during the process of purification after taking in original water. However, the interest in human health has been increased, so 'secondary contamination' ,polluted in the process of crossing water reservoir, distributing pipes, indoor water tanks and water pipes after water left a filtration plant, is soared up as an important object of water quality control. Especially the copper pipe for the tap water, its characteristic was acknowledged as a pipe with many merits such as excellent corrosion resistance and easy assembly at the time to lay, is being frequently used in the advanced countries like East Europe, North America, Australia, etc, and is also being used as the material of pipe laying among the pipes for distributing water in Germany and Netherlands. But in case of Korea, the blue or green water problem is found even in the less than 6 year old copper pipes after being installed newly in indoor pipe laying for water distributing that causes to bring about aesthetic distrust to citizens, so the necessity of study is raised to control this situation. In this study tried to control the corrosion and the blue and green water problem happening in the copper pipe using corrosion inhibitors to settle down the various problems available to happen by this problem and suggested an optimized method to manage this situation.

"In the current context of the existence of life, of the development of human activities, water has a double importance: - environmental factor, generator of ecological systems - “raw material” for certain uses (drinking water, industrial water, fish farming, leisure, etc.) The current problems in the field of water supply are due to: - exponential increase of water demand; - the limited water resources and their uneven distribution, which requires large and expensive works of development and accumulation of water; - deterioration of the quality of water sources, as a result of human activity and the emergence of industries that discharge waste, containing very stable impurities, difficult to remove from water, in the processes of water purification or treatment; - increase in standards on quality conditions that must be met by water delivered to the population [1]. For the extensive and intensive development of water supplies, a concrete solution, already existing in the area of Artificial Intelligence is given by heuristic methods and Evolutionary Calculus. This article provides an overview of the role of the most important metaheuristics, based on evolutionary concepts - Evolutionary computation and behavioral patterns inspired by biology - Swarm calculus) in the case of of water supply systems and their subsystems, with exemplification in the case of a model network (Scheme of the network distribution in Hanoi), taking into account water quality (its treatment with chlorine). "

Nearly two-third part of earth cover by water and this water is not for drinking from this only one percent is only for human use and which is not of sufficient to fulfill the human needs as water is the base of life so to overcome this water shortage problems there are different techniques which are helpful for the treatment of water like Desalination, phytoremediation, reverse osmosis, filtration, chlorination, coagulation and flocculation. As above-described processes solar water distillation is one of the most economic and renewable technique which is easily available everywhere. Many varieties of solar still are now developed regarding design like symmetric solar still, Asymmetric solar still, inclined single slope still, double slope still Steeped solar still and vacuum tube solar still etc. There are different natural factors which effect the yield of water purification like solar radiation, water depth in the basin, ambient temperature, and wind speed. These solar still are successful in arid atmosphere like in continent Africa and some parts of Asia where there is water shortage and irradiance values are higher there. Solar still working principle is to evaporate water in the basin and condense it on glass inner surface and then collect it in the collector. Conventional solar stills are less productive as compared to stills with vacuum tubes and steeped solar still in which internal reflectors were used.

The most shocking situation which is the cause of sorrow for the whole universe is Covid-19. This virus was first detected in Wuhan City, China. This pandemic has shown its ramification on us in different ways and in different ways in this world. We are recently facing the second wave of Covid-19. Till the date there is no full proof medicine for corona virus (SARS-CoV-2), but scientists around the world have succeeded in developing some vaccines which would help to protect us against the deadly virus (SAR-CoV-2). It is very much important for us to improve our physical as well as mental conditions, the best way to do so is by doing physical exercises, yoga , maintain proper diet, drinking plenty of water and maintain distance from others. Covid-19 mostly affects our lungs. People experience physical problems like breathing problem , chest pain, cold, fever etc. due to covid , and those people having serious symptoms are more prone to death. Yoga is one of the best way for improving the immunity as prescribed by several doctors, scientists, virologists and even by the scientists of WHO. Many Researches have been conducted and it shows yoga improves immunity, improves respiratory competence, mental power and increases body’s strength which is very much essential to fight against covid virus. In this study focus has been given on the importance of yoga in fighting against covid.

Modern sociology has a special look at the three associated variables of environment, water and energy. The three variables are not in a harmonial state in many parts of the globe. Some have access to two, or not sufficient to one. Only a few countries are in an equilibrium state of the three. For example, many African countries are in short fall of water and energy. What sociologists suggest is to bring about resources enough as far as the three parts are concerned. In the past, the threefold relationship was less considered and measured, but currently with the heavy weight of population over 7.8 billion world over (WPDS, 2020), balance between the three is inevitable. While population all over the world has increased considerably, water resources have not increased in the same way. Moreover, in the past, population dependency on energy was not that much. But, in the industrial age of today, man is highly in need of energy of different types to maintain life. However, waste and wastewater have become problematic in current age and in most parts of the world. The emerging situation is polluting environment, seas and water streams. It is more observable in less developed world than the developed world. Therefore, the water and energy crisis is wide and ongoing. It is discussed elaborately in the present article. However, national security could be accessible only if water-energy policies are there (Bauer et el. 2014). Introduction The threefold relationship of environment, water and energy is very important from a sociological point of view. Although in the past these three variables were less considered, and their relationship with each other has been less measured, at the same time, following the comprehensive development of modern societies, the tripartite relationship of these variables is inevitable today. To have a healthy environment, enough water resources and enough energy, you must always invest in it. While energy is highly dependent on water, the supply and transfer of water, and the disposal and transfer of wastewater also require energy. Therefore, water and energy, while being necessary for each other, also ensure the health and safety of individuals. Existence of lakes, dams and other similar sources generate energy through and with the power of these elements. At the same time, energy itself transports water resources from one region to another. It also happens with the energy power of the waste disposal system or system. Otherwise, the health of individuals and the health of society in general will face irreparable risks. In the past, when such facilities were less available, many health problems arose that eventually led to an increase in mortality. Therefore, in order to have a healthy environment, providing water and energy resources is very vital and inevitable. Likewise, drinking water itself needs energy for purification and purification operations, and re-pumping to consumers. This means that any interaction regarding the sanitation of water, its purification, its displacement, etc., is itself highly dependent on energy. These conditions ultimately lead to greater well-being, health and security. While developed societies have more or less achieved these possibilities over the last century or so, non-industrial societies have recently been able to implement such schemes; That is, a strategy that leads to better health for them. Where there is a shortage of clean drinking water, and water has to be transported over long distances, having energy is extremely important. Countries generally do not have the same amount of water resources for different uses. As a result, in many cases they have to move water from long distances to other places. This kind of movement requires sufficient and sustainable energy, and this makes agricultural exploitation, agricultural prosperity, access to more resources and products, and the like, more practical and achievable. One of the most significant challenges in this regard is within African countries; That is, areas that are generally short of energy, and the aforementioned losses have made it impossible for such communities to make good use of their potential resources (agricultural land); As a result, poverty and scarcity are widespread in such societies. Method of ResearchMethodology used in the present article is of qualitative type. In that, various paradigms have been used to find out about the facts regarding pandemics during the history. Qualitative research usually studies people, events or areas in their natural settings. In finding facts for the research, the researcher engaged in careful data collection and thoughtful analysis of what was relevant. In the documentary research applied for the present research, printed and written materials were widely regarded. The research was performed as a qualitative library-type in which the researcher had to refer to the relevant and related sources. In the current research, various documents were thoroughly investigated, and the needful inferences were made. The data fed by the investigator in the present article is hopefully reliable. Though literature on pandemics is very limited, yet the author tried to investigate many different resources in order to elicit the necessary information to build up the text. Energy and waterMany of the problems of the society will be reduced if all the people of a society have adequate access to energy and water. It means the safety of water for drinking and sanitary consumption (UNDP: 2015). Access to water and energy also greatly contributes to improving the quality of life. At the same time, access to these resources greatly contributes to the health of the environment, its preservation and maintenance. Today, many less developed communities face increasing population, population density, and mass migration to urban areas. They face water and energy constraints. This has caused the environment to be directly and indirectly affected, and in a negative way. Overpopulation in urban areas, on the one hand, and water scarcity, on the other, put many green space resources at risk of extinction. Therefore, urban environmental planners must always adjust and consider the relocation and resettlement of the population in accordance with water and energy resources. This statement can be applied to all human societies, and it means that energy and water are inseparable. For example, energy is inevitably needed to cool biofuels (hydropower) or water-based power plants, and so on, to access water sources or safe water. In other words, to transfer water from one area to another, or to pump water for change or desalination, we need sufficient and appropriate energy. Therefore, countries should always pay enough attention to these two sources in their planning path. However, many traditional water sources such as springs, aqueducts and the like are being destroyed in many communities. Likewise, following the general warming of the earth, water scarcity is felt more than ever in different communities. On the other hand, following the consumption of more and more population, the need for water directly and indirectly is always increasing. Given this scenario, environmental planners must always take new practical measures to meet the growing needs of their citizens. From a sociological point of view, basic human needs cannot be met without energy and water. That is, it provided food for the growing population, and sustained economic growth. Many societies today need more food, even than in previous years. In other words, more per capita should be considered for them in terms of food, services, agricultural resources and the like. This means that as the quality of life improves, so does the expectation of consumption. In such circumstances, the community in question needs more water resources. While many societies are in such a situation. Future consumption needs are less predictable. Rising prices for food and consumables around the world in recent years are evidence of this claim. That is, many societies around the world over the past decades have not paid attention to the current years (decades) of the 21st century. At the same time (today) (1.3 billion) 1.3 billion people in the world do not have access to electricity, and about 800 million people get their water from unhealthy sources. These conditions lead to many diseases, health problems, personal and social threats and other deprivations. Therefore, considering the natural trend of population growth, which is generally 2% per year or more in developing countries, the forecast and increase of water and energy resources is of crucial importance. As noted, nearly one-seventh of the world's population is now forced to use polluted water resources, which threatens the health of current and even future generations. Therefore, environmental sociologists must always measure and predict population growth index and water resources index together. Many African countries today are in such a situation. That is, a situation whose unhealthy conditions can be transferred to other communities. It's about the same billion people suffering from poverty, hunger and deprivation, and over the next thirty years the demand for food and energy will increase at an unprecedented rate. However, a high proportion of the population, or in other words one-seventh of the world's population, faces food deprivation. While by 2050 the world population will increase from the current 7.2 billion (2013) to more than 9.2 billion, during this time the expectations of individuals, their way of life and the different needs of citizens in different societies will also increase. . These conditions will further exacerbate food and energy problems. Therefore, social planners should distribute their urban and rural population in proportion to their water and energy resources. If more population pressure is applied to urban areas, it will put additional pressure on water and energy resources. However, many human societies today still rely on the same water resources to sustain their lives, economic growth and their environment. In a situation where the share of the population is increasing, effective and productive sources of agricultural and food production. That is, water and energy resources must also increase, otherwise many products. Food production, agricultural production and the like are more or less failing. Under such circumstances, more migration will inevitably occur, which in itself has a negative impact on the environment. This trend is more related to less developed countries than industrialized and developed countries. Improving communities and ecosystemsPutting water and energy on the agenda (from a systemic point of view). How it was developed and managed must be pursued at the local, national, regional and global levels. Water and energy as two influential and vital factors today should be regularly included in development plans, sufficient budgets should be allocated to them, and as mentioned, they should be pursued at different levels and in a participatory manner. In this way, water and energy supply can be achieved to some extent. Likewise, specialized departments, in partnership with other institutions, must make the necessary predictions in proportion to time and place. Therefore, water, energy and food supply will play a central role in the importance and environmental health of communities. Due to increasing population, urban population density, population growth, and changing lifestyles, the need for water, energy and food is felt more than ever in the past. Today, however, a significant portion of the world's population cannot easily meet these needs. Therefore, countries, both independently and in partnership with other communities, must meet the growing needs for water, energy and food as much as possible. In this way, the quality of life in these communities also improves. Many Third World countries, and African countries in general, face severe restrictions in the water, energy and food sectors. The issue of energy and water in general is important in two ways. That is, in terms of the opportunities and challenges of society, and the elimination of many of the growing needs in different societies. Water and energy, while creating opportunities, on the other hand, and in conditions of scarcity or scarcity, water inevitably brings challenges and limitations. Opportunities mean that in the conditions of having sufficient water and energy, economic-agricultural development takes place in its desired form. That is, a movement that itself provides more added value. With the opportunity in question, this situation will lead to more investment, more income, and ultimately more per capita GDP. That is, what leads to an improvement in the quality of life. Few countries have achieved this today. However, many developing societies today and in the years to come will face a water and energy crisis. The problem itself requires more studies, more investment and more international cooperation. Population, economy and energy and water demandThe production and use of energy and water in its national form is a significant necessity in order to meet the basic needs and develop opportunities for the people. Energy supply means access to clean, reliable and revenue-generating energy services for cooking, heating, lighting, communications and productive uses (United Nations: 2010). The supply of water resources and the production of energy required due to the growing needs, today is the first level of importance in different countries. The provision of these resources in its national and global form must be considered, otherwise uncontrolled migration from places without water and energy to other places will inevitably take place. That is, the flow that ultimately leads to environmental problems in various forms. This process leads to housing constraints, transportation problems, and many socio-economic disadvantages. Therefore, social planners, environmental sociologists, and economists must always have adequate oversight and effective forecasting in the water and energy sectors. Water and energy themselves provide food security. It means providing and accessing adequate, healthy and nutritious food that meets the daily nutritional needs and nutritional preferences for a healthy and active life (FAO: 1996). In any case, both energy and water cross national borders in some cases, thereby facilitating international cooperation. Today, following the need of countries for these two factors, new relations have emerged between countries. Whereas in the distant past, water currents flowed easily from one country to another, today for this movement. Contracts and treaties are concluded. Similarly, while countries today need more energy (for example, electricity), cooperation and areas of trade and transmission of electricity between countries are taking place. In this way, the fields of economic cooperation between countries have increased. That is, it provides conditions that improve agriculture, improve the environment, and provide more food, and so on. Cooperation between neighbors in this way provides benefits sharing, profitability, access to more food and water-related products. As the population of countries has increased in recent decades, and on the other hand, the need for food has increased, this has made the connection between countries more and more in terms of water and energy transmission. Is. Improved global water, energy and food supply conditions can be achieved through a cohesive policy. It means adopting a method in terms of management and administration, integrated in all sections and scales (WWF Retrieved). At the international level, ongoing crises such as energy, food, financial issues, and the like indicate systemic interdependence. If the needs related to the mentioned indicators such as energy, water and food are not met in an adequate level, the society will face various crises. Under such circumstances, the standard of living declines. That is, comfort, access to the required material goods, income, employment, domestic products, and inflation are all affected by the declining trend in living standards (Retrieved: 2011). For example, in the absence of energy and water, many villagers migrate to urban areas. That is, a movement that itself leads to crises such as environmental pollution, transportation, population density, destruction of the urban environment and the like. Such crises also lead to greater challenges to personal and social health. Therefore, the water and energy crisis poses many and ongoing challenges. Sociologists in general and environmental sociologists in particular evaluate and predict these conditions. Developing countries face serious challenges in achieving their Millennium Development Goals by 2015, and their close and intimate relationships with water, energy and food need to be re-examined to achieve the Millennium Development Goals. Developing countries must always anticipate their coming years by turning to water and energy resources. That is, such facilities that lead to the provision of food. However, such countries face unforeseen challenges and problems due to their increasing population on the one hand, and their extensive migration to urban areas on the other hand. Sociologists have always advised that greater individual and social health be achieved through access to adequate sources of water and energy, otherwise there will be many challenges in the lives of different strata. Likewise, the emergence of new injuries endangers individual and social health in various forms. Energy and water balanceEnergy and water are two important factors in urban development. Any industrial development and access to more industrial products, and more processing itself requires more energy and water resources. In the absence of these two sources, urban communities are largely exposed to economic stagnation, unemployment, and consequently economic inflation. This also leads to a decline in quality of life. Therefore, in proportion to the capacities related to their water and energy resources, they should welcome urban development. Today, many developing communities are facing this problem (restrictions on water and energy resources) in urban areas due to the general increase in their population, and migration from rural to urban areas. Fast-growing cities are heavily dependent on energy and water supply. But at the same time, they must reduce water demand, manage relevant trade, and make good use of their water resources. That is, through the reuse of water, the recycling of water and the production of energy from waste and the like. In a coherent and coordinated manner for industrial development, the use and reuse of energy and water is essential, in order to increase scarce resources and save costs. That is, during the production and management of waste, the motivation for social-environmental responsibility should be strengthened as much as possible through sustainable production. The relationship between energy and water is not only quantitative, but also water quality, water pollution, water pollution and the like must be considered. Different countries and societies, given their growing needs on the one hand, and the scarcity of water resources on the other hand, must always make multiple uses of the available water resources. It means recycling a lot of used water and reusing it in other fields and the like. Otherwise, the limitation and shortage of water resources will lead to food shortages. Therefore, continuous monitoring of its water resources to a large extent ensures the health and quality of life in urban and rural areas as much as possible. The connection between water and energy is inseparable, especially in urban areas. That is, city life depends on these two elements (Sustainca: 2015). Disseminate information on water and energyAccess to information and dissemination of data in the field of energy and water resources, or in other words, management of water resources, etc., is itself a major challenge in most societies today. Many countries, especially in less developed societies, do not have enough information about their water resources, water needs, future water resources, and water management in general. Therefore, based on estimates, such communities will sooner or later face challenges and problems due to water shortages. Therefore, from the sociological point of view of the environment, these communities should prioritize studies and information gathering in this regard as part of their plans, given the increase in their population and water consumption. Green infrastructure facilities, and nature conservation, provide significant services in protecting communities from floods and overheating, dust control, etc. It means strengthening green infrastructure (Benedict: 1947). The complexity of energy and water development decisions often requires some kind of modeling (or hybrid model), based on which an integrated support system is developed and maintained. To meet their water needs, countries must use newer and more advanced methods and models. Likewise, the link between less developed and more developed countries, in order to benefit from their experiences, can itself help in making decisions about energy and water development in less developed societies. Otherwise, the scope of the crisis will expand further in the coming years. Such developments include water and energy economics, their ecological impacts, social criteria, and economic tools that can be measured through choices. In other words, calculating and measuring their water and energy resources as effective methods help these countries in providing water resources. In general, today water and energy resources in its scientific form should be evaluated, measured and predicted. The bridge between science, politics and peopleDialogue or science, politics and people in the field of energy and water based on knowledge and education (literacy), indicates that energy and water need improvement and development. That is, effective efforts must be made in this regard. Innovations in technology, management and the like. In this way, a bridge between science, politics and people can be created. By creating such a tripartite relationship, energy and water resources can be fundamentally managed. At the same time, science and technology must be aligned with, and aligned with, energy and water policy.Otherwise, the challenges and shortcomings of energy and water constraints will become more and more widespread. In a situation where the global population has increased to more than 7.2 billion people today, and at the same time social, economic, service and similar needs have increased more than ever in the past, the use of science and technology to Providing as much energy and water as possible is inevitable. This connection can also be explained by the fact that human beings are inseparable from nature. As any damage to nature by man, man himself is subsequently harmed (Rights of Mother Earth: 2011). As far as developing countries are concerned, such efforts should be made to expand capacities at all levels. By creating such connections or putting them on the agenda, the necessary coordination between the environment, water and energy is achieved. Therefore, capacity building at different levels, including urban and rural areas, industrial and agricultural capacity, human capacity, both men and women, each play a role in providing resources related to water, energy and a healthy environment. ConclusionSociologically speaking, basic human needs cannot be met without energy and water. Currently, over 1.3 billion people in the world do not have access to electricity, and over 800 million people get their water from unhealthy sources. Such conditions lead to many diseases, health problems, personal and social threats, and other deprivations. As noted earlier, one-seventh of world's population is currently forced to use polluted water resources which threatens the health of generations. Therefore, environmental sociologists must always measure and predict the population growth index and water resources index together. However, many human societies still rely on the same water resources to sustain their lives, their economic growth and their environment. Water and energy as two influential and vital factors should be regularly included in development plans, and sufficient budgets need to be allocated to them. Eventually, it must be noted that water, energy and food supply play a central role in the environmental health of communities. References: Bauer, D.; et al. "The Water-Energy Nexus: Challenges and Opportunities". US Department of Energy. 2014. Benedict, M.A.; et al. Green Infrastructure: Linking Landscapes and Communities. 1947. California Sustainability Alliance, Cynthia, Truelove, Senior Water Policy Analyst, California Public Utilities Commission. FAO. Rome Declaration on World Food Security and World Food Summit Plan of Action, World Food Summit 13-17, November 1996, Rome. Nexus Resource. Right of Mother Earth, Bolivia UN, Bolovian.net, Retrieved 2011. Standard of Living Definition, Investopedia.com, Retrieved 2011. UNDP: Millennium Development Goals, Goal 7: Ensure Environmental Sustainability. 2015. UN Secretary General's Advisory Group on Energy and Climate Change (AGECC), Summary Report and Recommendations, 28 April 2010, P.13. World Population Data Sheet, Population Reference Bureau, Washington DC. 2020.

Our studies have indicated that the relative concentration of Se or Hg to As in urine and blood positively correlates with percentage of inorganic arsenic (% Inorg-As) and percentage of monomethlyarsonic acid [% MMA (V)]. We also found a negative correlation with percentage of dimethylarsinic acid [% DMA (V)] and the ratio of % DMA (V) to % MMA (V). In another study, we found that a group of proteins were significantly over expressed and conversely other groups were under-expressed in tissues in Na-As (III) treated hamsters. Introduction.Inorganic arsenic (Inorg-As) in drinking water.One of the largest public health problems at present is the drinking of water containing levels of Inorg-As that are known to be carcinogenic. At least 200 million people globally are at risk of dying because of arsenic (As) in their drinking water1-3. The chronic ingestion of Inorg-As can results in skin cancer, bladder cancer, lung cancer, and cancer of other organs1-3. The maximum contamination level (MCL) of U.S. drinking water for arsenic is 10 ug/L. The arsenic related public health problem in the U.S. is not at present anywhere near that of India4, Bangladesh4, and other countries5. Metabolism and toxicity of Inorg-As and arsenic species.Inorg-As is metabolized in the body by alternating reduction of pentavalent arsenic to trivalent form by enzymes and addition of a methyl group from S-adenosylmethionine6, 7; it is excreted mainly in urine as DMA (V)8. Inorganic arsenate [Inorg-As (V)]is biotransformed to Inorg-As (III), MMA (V), MMA (III), DMA (V), and DMA (III)6(Fig. 1). Therefore, the study of the toxicology of Inorg-As (V) involves at least these six chemical forms of arsenic. Studies reported the presence of 3+ oxidation state arsenic biotransformants [MMA (III) and DMA (III)] in human urine9and in animal tissues10. The MMA (III) and DMA (III) are more toxic than other arsenicals11, 12. In particular MMA (III) is highly toxic11, 12. In increased % MMA in urine has been recognized in arsenic toxicity13. In addition, people with a small % MMA in urine show less retention of arsenic14. Thus, the higher prevalence of toxic effects with increased % MMA in urine could be attributed to the presence of toxic MMA (III) in the tissue. Previous studies also indicated that males are more susceptible to the As related skin effects than females13, 15. A study in the U.S population reported that females excreted a lower % Inorg-As as well as % MMA, and a higher % DMA than did males16. Abbreviation: SAM, S-adenosyl-L-methionine; SAHC, S-adenosyl-L-homocysteine. Differences in susceptibility to arsenic toxicity might be manifested by differences in arsenic metabolism among people. Several factors (for examples, genetic factors, sex, duration and dosage of exposure, nutritional and dietary factors, etc.) could be influence for biotransformation of Inorg-As,6, 17 and other unknown factors may also be involved. The interaction between As, Se, and Hg.The toxicity of one metal or metalloid can be dramatically modulated by the interaction with other toxic and essential elements18. Arsenic and Hg are toxic elements, and Se is required to maintain good health19. But Se is also toxic at high levels20. Recent reports point out the increased risk of squamous cell carcinoma and non-melanoma skin cancer in those treated with 200 ug/day of selenium (Nutritional Prevention of Cancer Trial in the United States)21. However, it is well known that As and Se as well as Se and Hg act as antagonists22. It was also reported that Inorg-As (III) influenced the interaction between selenite and methyl mercury23. A possible molecular link between As, Se, and Hg has been proposed by Korbas et al. (2008)24. The identifying complexes between the interaction of As and Se, Se and Hg as well as As, Se, and Hg in blood of rabbit are shown in Table 1. Influence of Se and Hg on the metabolism of Inorg-As.The studies have reported that Se supplementation decreased the As-induced toxicity25, 26. The concentrations of urinary Se expressed as ug/L were negatively correlated with urinary % Inorg-As and positively correlated with % DMA27. The study did not address the urinary creatinine adjustment27. Other researchers suggested that Se and Hg decreased As methylation28-31(Table 2). They also suggested that the synthesis of DMA from MMA might be more susceptible to inhibition by Se (IV)29 as well as by Hg (II)30,31 compared to the production of MMA from Inorg-As (III). The inhibitory effects of Se and Hg were concentration dependent28-31. The literature suggests that reduced methylation capacity with increased % MMA (V), decreased % DMA (V), or decreased ratios of % DMA to % MMA in urine is positively associated with various lesions32. Lesions include skin cancer and bladder cancer32. The results were obtained from inorganic arsenic exposed subjects32. Our concern involves the combination of low arsenic (As) and high selenium (Se) ingestion. This can inhibit methylation of arsenic to take it to a toxic level in the tissue. Dietary sources of Se and Hg.Global selenium (Se) source are vegetables in the diet. In the United States, meat and bread are the common source. Selenium deficiency in the US is rare. The US Food and Drug Administration (FDA) has found toxic levels of Se in dietary supplements, up to 200 times greater than the amount stated on the label33. The samples contained up to 40,800 ug Se per recommended serving. For the general population, the most important pathway of exposure to mercury (Hg) is ingestion of methyl mercury in foods. Fish (including tuna, a food commonly eaten by children), other seafood, and marine mammals contain the highest concentrations. The FDA has set a maximum permissible level of 1 ppm of methyl mercury in the seafood34. The people also exposed mercury via amalgams35. Proteomic study of Inorg-As (III) injury.Proteomics is a powerful tool developed to enhance the study of complex biological system36. This technique has been extensively employed to investigate the proteome response of cells to drugs and other diseases37, 38. A proteome analysis of the Na-As (III) response in cultured lung cells found in vitro oxidative stress-induced apoptosis39. However, to our knowledge, no in vivo proteomic study of Inorg-As (III) has yet been conducted to improve our understanding of the cellular proteome response to Inorg-As (III) except our preliminary study 40. Preliminary Studies: Results and DiscussionThe existing data (Fig. 1) from our laboratory and others show the complex nature of Inorg-As metabolism. For many years, the major way to study, arsenic (As) metabolism was to measure InorgAs (V), Inorg-As (III), MMA (V), and DMA (V) in urine of people chronically exposed to As in their drinking water. Our investigations demonstrated for the first time that MMA (III) and DMA (III) are found in human urine9. Also we have identified MMA (III) and DMA (III) in the tissues of mice and hamsters exposed to sodium arsenate [Na-As (V)]10, 41. Influence of Se as well as Hg on the As methyltransferase.We have reported that Se (IV) as well as mercuric chloride (HgCl2) inhibited As (III) methyltransferase and MMA (III) methyltransferase in rabbit liver cytosol. Mercuric chloride was found to be a more potent inhibitor of MMA (III) methyltransferase than As (III) methyltransferase30. These results suggested that Se and Hg decreased arsenic methylation. The inhibitory effects of Se and Hg were concentration dependent30. Influence of Se and Hg in urine and blood on the percentage of urinary As metabolites.Our human studies indicated that the ratios of the concentrations of Se or Hg to As in urine and blood were positively correlated with % Inorg-As and % MMA (V). But it negatively correlated with % DMA (V) and the ratios of % DMA (V) to % MMA (V) in urine of both males and females (unpublished data) (Table 3). These results confirmed that the inhibitory effects of Se as well as Hg for the methylation of Inorg-As in humans were concentration dependent. We also found that the concentrations of Se and Hg were negatively correlated with % Inorg-As and % MMA (V). Conversely it correlated positively with % DMA (V) and the ratios of % DMA (V) to % MMA (V) in urine of both sexes (unpublished data). These correlations were not statistically significant when urinary concentrations of Se and Hg were adjusted for urinary creatinine (Table 3). Interactions of As, Se, Hg and its relationship with methylation of arsenic are summarized in Figure 2. Sex difference distribution of arsenic species in urine.Our results indicate that females have more methylation capacity of arsenic as compared to males. In our human studies (n= 191) in Mexico, we found that females (n= 98) had lower % MMA (p<0.001) and higher % DMA (p=0.006) when compared to males (n= 93) (Fig. 3). The means ratio of % MMA (V) to % Inorg-As and % DMA (V) to %MMA (V) were also lower (p<0.05) and higher (p<0.001), respectively in females compared to males. The protein expression profiles in the tissues of hamsters exposed to Na-As (III).In our preliminary studies40, hamsters were exposed to Na-As (III) (173 pg/ml as As) in their drinking water for 6 days and control hamsters were given only the water used to make the solutions for the experimental animals. After DIGE (Two-dimensional differential in gel electrophoresis) and analysis by the DeCyder software, several protein spots were found to be over-expressed (red spot) and several were under expressed (green spot) as compared to control (Figs. 4a-c). Three proteins (one was over-expressed and two were under-expressed) of each tissue (liver and urinary bladder) were identified by LC-MS/MS (liquid chromatography-tandem mass spectrometry).DIGE in combination with LC-MS/MS is a powerful tool that may help cancer investigators to understand the molecular mechanisms of cancer progression due to Inorg-As. Propose a new researchThese results suggested that selenium (Se) as well as mercury (Hg) may influence the methylation of Inorg-As and this influence could be dependent on the concentration of Se, Hg and/or the sex of the animal. Our study also suggested that the identification and functional assignment of the expressed proteins in the tissues of Inorg-As (III) exposed animals will be useful for understanding and helping to formulate a theory dealing with the molecular events of arsenic toxicity and carcinogenicity.Therefore, it would be very useful if we could do a research study with combination of Inorg-arsenic, Se, and Hg. The new research protocol could be the following:For metabolic processing, hamsters provide a good animal model. For carcinogenesis, mouse model is well accepted. The aims of this project are: 1) To map the differential distributions of arsenic (As) metabolites/species in relation to selenium (Se) and mercury (Hg) levels in male and female hamsters and 2) To chart the protein expression profile and identify the defense proteins in mice and hamsters after As injury. Experimental hamsters (male or female) will include four groups. The first group will be treated with Na arseniteNa-As(III), the second group with Na-As (III) and Na-selenite (Na-Se (IV)], the third group with Na As (III) and methyl mercuric chloride (MeHgCl), and the final group with Na-As (III), Na-Se (IV), and MeHgci at different levels. Urine and tissue will be collected at different time periods and measured for As species using high performance liquid chromatography/inductively coupled plasma-mass spectrometry (HPLC/ICP-MS). For proteomics, mice (male and female) and hamsters (male and female) will be exposed to Na-As (III)at different levels in tap water, and control mice and hamsters will be given only the tap water. Tissue will be harvested at different time periods. TWO dimensional differential in gel electrophoresis (2D-DIGE) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) will be employed to identify the expressed protein. In summary, we intend to extend our findings to: 1) Differential distribution of As metabolites in kidney, liver, lung, and urinary bladder of male and female hamsters exposed to Na-As (III), and combined with Na-As (III) and Na-Se (IV) and/or MeHgCl at different levels and different time periods, 2) Show the correlation of As species distribution in the tissue and urine for both male and female hamsters treated with and without Na-Se (IV) and/or MeHgCl, and 3) Show protein expression profile and identify the defense proteins in the tissues (liver, lung, and urinary bladder epithelium) in mice after arsenic injury. The significance of this study: The results of which have the following significances: (A) Since Inorg-As is a human carcinogen, understanding how its metabolism is influenced by environmental factors may help understand its toxicity and carcinogenicity, (B) The interactions between arsenic (As), selenium (Se), and mercury (Hg) are of practical significance because populations in various parts of the world are simultaneously exposed to Inorg-As & Se and/or MeHg, (C) These interactions may inhibit the biotransformation of Inorg-As (III) which could increase the amount and toxicity of Inorg-As (III) and MMA (III) in the tissues, (D) Determination of arsenic species profile in the tissues after ingestion of Inorg-As (III), Se (IV), and/or MeHg+ will help understand the tissue specific influence of Se and Hg on Inorg-As (III) metabolism, (E) Correlation of arsenic species between tissue and urine might help to understand the tissue burden of arsenic species when researchers just know the distribution of arsenic species in urine, (F) The identification of the defense proteins (over-expressed and under-expressed) in the tissues of the mouse may lead to understanding the mechanisms of inorganic arsenic injury in human. The Superfund Basic Research Program NIEHS Grant Number ES 04940 from the National Institute of Environmental Health Sciences supported this work. Additional support for the mass spectrometry analyses was provided by grants from NIWHS ES 06694, NCI CA 023074 and the BIO5 Institute of the University of Arizona. Acknowledge:The Authorwantsto dedicate this paper to the memory of Dr. H. VaskenAposhian and Dr. Mary M. Aposhian who collected urine and bloodsamples from Mexican population. The work was done under Prof. H. V. Aposhian sole supervision and with his great contribution. References NRC (National Research Council). Arsenic in Drinking Water. Update to the 1999 Arsenic in Drinking Water Report. National Academy Press, Washington, DC. 2001. Gomez-Caminero, A.; Howe, P.; Hughes, M.; Kenyon, ; Lewis, D. R.; Moore, J.; Mg, J.; Aitio, A.; Becking, G. Environmental Health Criteria 224. Arsenic and Arsenic Compounds (Second Edition). International Programme on Chemical Safety, World Health Organization. 2001. Chen, C. J.; Chen, C. W.; Wu, M.; Kuo, T. L. Cancer potential in liver, lung, bladder, and kidney due to ingested inorganic arsenic in drinking water. Br. J. Cancer. 1992, 66, 888-892. Chakraborti, D.; Rahman, M.; Paul, K.; Chowdhury, U. K.; Sengupta, M. K.; Lodh, D.; Chanda, C. R.; Saha, K. C.; Mukherjee, S. C. Arsenic calamity in the Indian subcontinent. What lessons have been learned? 2002, 58, 3-22. Nordstrom, D. K. Worldwide occurrences of arsenic in ground water. Scienc 2002, 296, 2143-2145. Aposhian, H. V.; Aposhian, M. M. Arsenic toxicology: five question Chem. Res. Toxicol. 2006, 19, 1-15. Aposhian, H. V. Enzymatic methylation of arsenic species and other new approaches to arsenic toxicity. An Rev. Pharmacol. Toxicol. 1997, 37, 397-419. Vahter, M. Variation in human metabolism of arsenic. In: Abernathy, C. O.; Calderon, R. L.; Chappell, W. R., (eds) Arsenic exposure and Health effect Elsevier Science, New York, 1999, pp 267-279. Aposhian, H. V., Gurzau, E. , Le, X. C., Gurzau, A., Healy, S. M., Lu, X., Ma, M., Yip, L., Zakharyan, R. A., Maiorino, R. M., Dart, R. C., Tircus, M. G., Gonzalez-Ramirez, D., Morgan, D. L., Avram, D., Aposhian, M. M. (2000). Occurrence of monomethylarsonous acid in urine of humans exposed to inorganic arsenic. Chem. Res. Toxicol. 13, 693-697. ; U. K.; Zakharyan, R. A.; Hernandez, A.; Avram, M.D.; Kopplin, M. J.; Aposhian, H. V. Glutathione-S-transferase-omega [MMA (V) reductase] knockout mice: Enzyme and arsenic species concentrations in tissues after arsenate administration. Toxicol. Appl. Pharmacol. 2006, 216, 446-457. Styblo, M.; Del Razo, L. M.; Vega, L.; Germolec, D. R.; LeCluyse, E. L.; Hamilton, G. A.; Reed, W.; Wang, C.; Cullen, W. R.; Thomas, D.J. Comparative toxicity of trivalent and pentavalent inorganic and methylated arsenicals in rat and human cells. A Toxicol., 2000, 74, 289-299. Petrick, J. S.; Jagadish, B.; Mash, E. A.; Aposhian, H. V. Monomethylarsonous acid (MMAIII) and arsenite: LD50 in hamsters and in vitro inhibition of pyruvate dehydrogenase. Ch Res. Toxicol. 2001, 14, 651-656. Lindberg, A. L.; Rahman, M.; Persson, L. A.; Vahter, M. The risk of arsenic induced skin lesions in Bangladeshi men and women is affected by arsenic metabolism and the age at first exposure. Appl. Pharmacol. 2008, 230, 9-16. Vahter, M. Mechanisms of arsenic biotransformation. Toxicolog 2002, 181-182, 211-217. Chen, Y. C.; Guo, Y. L.; Su, H. J.; Hsueh, Y. M.; Smith, T. J.; Ryan, L. M.; Lee, M. S.; Chao, S. C.; Lee, J. Y.; Christiani, D. C. Arsenic methylation and skin cancer risk in southwestern Taiwan. Occup. Environ. Med. 2003, 45, 241-248. Steinmaus, C.; Carrigan, K.; Kalman, D.; Atallah, R.; Yuan, Y.; Smith, A.H. Dietary intake and arsenic methylation in a U.S. population. Health Perspect. 2005, 113, 1153-1159. Tseng, C. H. A review on environmental factors regulating arsenic methylation in humans. Appl. Pharmacol. 2009, 235, 338-350. Goyer, R. A. Factors influencing metal toxicity. In: Goyer, R. A.; Klaassen, C. D.; Waalkes, M. P. (eds) Metal toxicolog Academic Press, San Diego, 1995, pp 31-45. Wilber, C. G. Toxicology of selenium. Toxicol. 1980, 17, 171-230. Skerfving, S. Interaction between selenium and methylmercury. Environ. Health Persp 1978, 25, 57-65. Duffield-Lillico, A. J.; Slate, E. H.; Reid, M. E.; Turnbull, B. W.; Wilkins, P. A.; Combs, G. F.; Kim Park, Jr. H.; Gross, E. G.; Graham, G. F.; Stratton, M. S.; Marshall, J. R.; Clark, L. C. Selenium supplementation and secondary prevention of nonmelanoma skin cancer in a randomized trial. Natl. Cancer Inst. 2003, 95, 1477-1481. Gailer, J. Arsenic-selenium and mercury-selenium bonds in biology. Chem. Rev. 2007, 251, 234-254. Alexander, J. The influence of arsenite on the interaction between selenite and methyl mercury. Dev. Toxicol. Environ. Sci. 1980, 8, 585-590. Korbas, M.; Percy, J.; Gailer, J.; George, G. N. A possible molecular link between the toxicological effects of arsenic, selenium and methyl mercury: methyl mercury (II) selenobis (S glutathionyl) arsenic (III). J. Biol. Inorg. Chem. 2008, 13, 461-470. Yang, ; Wang, W.; Hou, S.; Peterson, P. J.; Williams, W. P. Effect of selenium supplementation on arsenism: an intervention trial in Inner Mongolia. Environ. Geochem. Health. 2002, 24, 359-374. Verret, W. J.; Chen, Y.; Ahmed, A.; Islam, T.; Parvez, F.; Kibriya, M. G.; Graziano, J. H.; Ahsan, H. Effects of vitamin E and selenium on arsenic-induced skin lesions. Occup. Environ. Med. 2005, 47, 1026-1035. Hsueh, Y. M.; Ko, Y. F.; Huang, Y. K.; Chen, H. W.; Chiou, H. Y.; Huang, Y. L.; Yang, M. ; Chen, C. J. Determinants of inorganic arsenic methylation capability among residents of the Lanyang Basin, Taiwan: arsenic and selenium exposure and alcohol consumption. Toxicol. Lett. 2003, 137, 49-63. Kenyon, E. M.; Hughes, M. K.; Levander, 0. Influence of dietary selenium on the disposition of arsenate in the female B6C3F1 mouse. J. Toxicol. Environ. Health. 1997, 51, 279-299. Styblo, M.; Thomas, D, J. Selenium modifies the metabolism and toxicity of arsenic in primary rat hepatocytes. Toxicol Appl. Pharmacol. 2001, 172, 52-61. Zakharyan, R.; Wu, Y.; Bogdan, G. M.; Aposhian, H. V. Enzymatic methylation of arsenic compounds: assay, partial purification, and properties of arsenite methyltransferase and monomethylarsonic acid methyltransferase of rabbit liver. Res. Toxicol.1995, 8, 1029-1038. Styblo, M.; Delnomdedieu, M.; Thomas, D. J. Mono- and dimethylation of arsenic in rat liver cytosol in vitro. -Biol. Interact. 1996, 99, 147-164. Tseng C. H. Arsenic methylation, urinary arsenic metabolites and human diseases: current perspective. J. Environ. Sci. Health Part C. 2007, 25, 1-22. FDA (The US Food and Drug administration). (2008). Hazardous levels of selenium in samples of "Total Body Formula" and "Total Body Mega Formula”. FDA Ne 2008. ATSDR (Agency for Toxic Substances and Disease Registry). Toxicological profile for mercury (CAS # 7439-97-6). Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service. 1999. Dye, B. A.; Schober, S. E.; Dillon, C. F.; Jones, R. L.; Fryar, C.; McDowell, M.; Sinks, T. H. Urinary mercury concentrations associated with dental restorations in adults women aged 16-49 years: United States, 1999-2000. O Environ. Med. 2005, 62, 368-375. Lau, A. T.; He, Q. Y.; Chiu, J. F. Proteomic technology and its biomedical applications. A Biophys. Sin. 2003, 35, 965-975. Jungblut, P. R.; Zimny-Arndt, U.; Zeindl-Eberhart, E.; Stulik, J.; Koupilova, K.; Pleissner, K. P.; Otto, A.; Muller, E. C.; Sokolowska-Kohler, W.; Grabher, G.; Stoffler, G. Proteomics in human disease: cancer, heart and infectious diseases. Electrophoresis. 1999, 20, 2100-2110. Hanash, S. M.; Madoz-Gurpide, J.; Misek, D. E. Identification of novel targets for cancer therapy using expression proteomics. L 2002, 16, 478-485. Lau, A. T.; He, Q. Y.; Chiu, J. F. A proteome analysis of the arsenite response in cultured lung cells: evidence for in vitro oxidative stress-induced apoptosis. J. 2004, 382, 641-650. Chowdhury, U. K.; Aposhian, H. V. Protein expression in the livers and urinary bladders of hamsters exposed to sodium arsenite. A N. Y. Acad. Sci. 2008, 1140, 325-334. Sampayo-Reyes, A.; Zakharyan, R. A.; Healy, S. M.; Aposhian, A. V. Monomethylarsonic acid reductase and monomethylarsonou acid in hamster tissue. Chem. Res. Toxicol. 2000, 13, 1181-1186.