Thematische Bibliographien / Diseases and pests

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Auswahl der wissenschaftlichen Literatur zum Thema „Diseases and pests“

Autor: Grafiati

Veröffentlicht am 4. Juni 2021

Zuletzt aktualisiert am 30. Juli 2024

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Zeitschriftenartikel zum Thema "Diseases and pests"

Raman, K. V. „SURVEY OF DISEASES AND PESTS IN AFRICA: PESTS“. Acta Horticulturae, Nr. 213 (September 1987): 145–50. http://dx.doi.org/10.17660/actahortic.1987.213.15.

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Heitefuss, Rudolf. „Wheat Diseases and Pests“. Journal of Phytopathology 159, Nr. 4 (05.10.2010): 324. http://dx.doi.org/10.1111/j.1439-0434.2010.01750.x.

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Muminova, R. „Malus Pests and Diseases“. Bulletin of Science and Practice, Nr. 5 (15.05.2023): 175–80. http://dx.doi.org/10.33619/2414-2948/90/22.

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The Malus is a deciduous tree of the Rosaceae family, which is actively used in horticulture. In order to maintain healthy, productive trees and fruits, it is necessary to know what pests and control measures exist. The paper presents a review of modern literature on the means and methods of combating diseases and pests of the apple tree. In conclusion, practical conclusions are given about the most effective methods of struggle.

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Muminova, Ra`no Dalabaevna. „APPLE PESTS AND DISEASES“. Theoretical & Applied Science 120, Nr. 04 (30.04.2023): 33–36. http://dx.doi.org/10.15863/tas.2023.04.120.8.

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Bukhonova, Yu V., und N. G. Mikhina. „Monitoring pests and diseases of linen“. Защита и карантин растений, Nr. 5 (2022): 25–29. http://dx.doi.org/10.47528/1026-8634_2022_5_25.

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Bukhonova, Yu V., und N. G. Mikhina. „Monitoring of legume pests and diseases“. Защита и карантин растений, Nr. 3 (2022): 22–26. http://dx.doi.org/10.47528/1026-8634_2022_3_22.

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Heitefuss, Rudolf. „Compendium of Hop Diseases and Pests“. Journal of Phytopathology 158, Nr. 5 (Mai 2010): 391. http://dx.doi.org/10.1111/j.1439-0434.2009.01617.x.

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Heitefuss, Rudolf. „Compendium of Beet Diseases and Pests“. Journal of Phytopathology 158, Nr. 5 (Mai 2010): 392. http://dx.doi.org/10.1111/j.1439-0434.2009.01626.x.

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Kidd, Hamish, und Len Copping. „BCPC Conference 2000 - Pests and Diseases“. Pesticide Outlook 12, Nr. 1 (2001): 36–39. http://dx.doi.org/10.1039/b100807m.

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Mikhina, N. G., und Yu V. Bukhonova. „Monitoring of cabbage pests and diseases“. Защита и карантин растений, Nr. 12 (2022): 14–19. http://dx.doi.org/10.47528/1026-8634_2022_12_14.

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Dissertationen zum Thema "Diseases and pests"

Terry, Alan J. „Control of pests and diseases“. Thesis, University of Surrey, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.505965.

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George, Adrienne. „Cataloguing Diseases and Pests in Captive Corals“. Scholar Commons, 2011. http://scholarcommons.usf.edu/etd/3115.

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Stony corals (Order Scleractinia) are susceptible to a variety of diseases, which can occur from abiotic or biotic factors, or a combination of both. Public aquaria provide opportunities to study coral disease. Because coral mucus is the first line of defense against disease, the Biolog EcoplateTM is a useful tool to detect differences in microbial assemblages in the surface mucopolysaccharide (mucus) layer when comparing healthy and diseased corals. Histological examination is essential to document structural changes in coral tissue in response to diseases. This study identifies and characterizes diseases in captive corals through visual recognition, characterization of carbon utilization by microbial assemblages in coral mucus samples, and histological examination. In March 2010, surveys were sent to public aquaria throughout the United States that house corals. If the survey was returned indicating that the aquarium had diseased coral specimens present, sample kits were sent to the aquarium to acquire photographs, mucus samples for microbial carbon utilization analysis, and tissue samples for histological examination. Eighteen aquaria participated in the survey and 25 sets of samples were provided. The gross lesions from diseased samples fit into six categories: discoloration associated with darkening of the tissue or with color loss (bleaching), growth anomalies, and tissue loss associated with pests, with brown jelly, or with no obvious cause. Seven categories of possible contributing factors were reported: addition of inadequately quarantined corals to the tank, damage during transport, change of location, manual stress, and variations in light, salinity, or temperature. Introduction of inadequately quarantined specimens was the most common possible contributing factor to pest introduction. Significant differences in carbon source utilization were found between tank-water samples and mucus from both healthy and diseased areas of sampled corals. Although mucus samples from healthy and diseased samples did not differ in carbon source utilization overall, D-mannitol was used by 52% of microbial assemblages from mucus from diseased areas compared with only 17% of microbial assemblages from healthy mucus samples. Histologically, the most commonly observed features across all samples were healthy zooxanthellae, endolithic organisms, and nematocysts, all of which are normal features that can be influenced by stress factors. Brown granular material and ciliates were found associated with some anomalies, primarily the three categories of tissue loss. The presence of dense aggregates of zooxanthellate-engorged ciliates in corals afflicted with brown jelly was highly similar to histological observations of brown band syndrome, previously described from natural coral reefs.

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Melander, Margareta. „Transgenic resistance to pathogens and pests /“. Alnarp : Dept. of Crop Science, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/a496.pdf.

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Marino, Dante. „Screening of Germplasm Accessions from the Brassica Species for Resistance against PG3 and PG4 Isolates of Blackleg“. Thesis, North Dakota State University, 2011. https://hdl.handle.net/10365/29053.

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Blackleg is a disease of canola and rapeseed cultivars that is caused by the fungus Leptosphaeria maculans (Desm.) Ces. & de Not., and it is by far the most destructive pathogen of canola in North America. In recent years, blackleg strains belonging to pathogenicity groups (PG) 3 and 4 have been discovered in North Dakota. Recent outbreaks of the disease have added a sense of urgency to characterize the risk these new strains represent for the canola industry and to identify sources of resistance against them. Thus, the objectives of this study were to screen germplasm collections of Brassica rapa, B. napus. and B. juncea for their reaction to PG3 and PG4 and to evaluate the reaction of a sample of currently used canola commercial cultivars grown in North Dakota to PG3 and PG4 as means to estimate the risk these new strains represent. All canola germplasm and commercial cultivars were evaluated in replicated trials in greenhouse conditions using cotyledon bioassays. In 2009 and 2010, the effect of these strains, using five inoculation sequences, on the reaction of canola seedlings was also evaluated. Field trials were not conducted because of the limited geographical distribution of the new strains. No adequate sources of resistance were identified among the 277 B. rapa and 130 B. napus accessions evaluated; however, 22 of the 406 accessions of Brassicajuncea evaluated were considered to have moderate levels of resistance. B. juncea seedlings that survived these inoculations were self-pollinated and their progeny (F1) were also screened. As before, surviving seedlings were self-pollinated. These F2 seeds are the elite materials that could be used in future breeding programs. The complementary study evaluating the role of sequence inoculations in reaction of canola seedlings to blackleg indicated that an increased susceptibility to PG3 occurred when seedlings were first inoculated with PG4; however, reaction to PG4 was not enhanced by a prior inoculation with PG3. All 75 commercial cultivars evaluated were susceptible to PG3 and PG4, indicating that the risk these new strains represent to the canola industry of the region is serious. Further, when a subsample of 16 cultivars were challenged with PG2, they were either resistant or moderately resistant, suggesting the ratings the industry are using relate to reaction of those cultivars to PG2 but not to the new strains; thus, growers should use caution when using these ratings while deciding on which cultivars to plant.
North Dakota State University. Department of Plant Pathology
USDA North Central Canola Research Program
Northern Canola Growers Association

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Denman, Sandra. „Botryosphaeria diseases of proteaceae“. Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52721.

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Dissertation (PhD (Agric))--University of Stellenbosch, 2002.
ENGLISH ABSTRACT: Fungi belonging to the genus Botryosphaeria are heterotrophic micromycetes that can be pathogens on woody plants. They cause serious, and in some cases devastating losses to crops through leaf necrosis, stem cankers and plant death. The Proteaceae cut-flower industry in South Africa accounts for 70% of the national cut-flower enterprise. Botryosphaeria diseases are a major impediment to production and trade of Proteaceae and there is an urgent need to investigate the etiology, epidemiology and control of these diseases. Losses of one of the most important proteas, P. magnifica, amount to 50% or more, locally. The main aims of this study were therefore to establish the etiology and aspects of epidemiology of Botryosphaeria stem cankers on P. magnifica and other Proteaceae, and to investigate methods of disease control. Although there is a vast body of information pertaining to this fungus, which was reviewed in Chapter 1, there is relatively little information available on Botryosphaeria on Proteaceae. The taxonomy of Botryosphaeria requires thorough review, and molecular techniques need to be employed to resolve species identities. In Chapter 2, it was found that Phyllachora proteae, a leaf pathogen of proteas, produced a Fusicoccum anamorph, which is described as F. proteae. A sphaeropsis-like synanamorph was associated with F. proteae and a new combination for P. proteae is proposed in Botryosphaeria, as B. proteae. The taxonomy of Botryosphaeria is in disarray at both the generic and the specific level. In Chapter 3 the taxonomic history of Botryosphaeria is reviewed, and the genus circumscribed and distinguished from other morphologically similar genera. Although several anamorph genera have been linked to Botryosphaeria, based on morphological observations and phylogenetic analysis of lTS rDNA sequence data, two anamorph genera are now recognised, those with pigmented conidia (Diplodia), and those with hyaline conidia (Fusicoccum). Botryosphaeria proteae should thus be excluded from Botryosphaeria. Several pathogenic Botryosphaeria spp. have an endophytic phase within their hosts. They are therefore imported unwittingly into other countries where they may pose a risk to agriculture and indigenous vegetation. The current global distribution of Botryosphaeria spp. associated with Proteaceae is clarified and a key to these taxa associated with Proteaceae is provided in Chapter 4. Five Botryosphaeria spp. are associated with cut-flower Proteaceae worldwide viz. B. lute a, B. obtusa, B. protearum, B. proteae and B. rib is. B. protearum is described as a new species. A thorough understanding of disease epidemiology is essential to effect a reduction of losses. In Chapter 5, I show that on P. magnifica, lesions caused by Botryosphaeria protearum, which lead to the formation of stem cankers, are initiated in the mid-rib vein or margin of leaves. Koch's postulates were satisfied and it was found that the number of lesions that developed from artificial inoculations correlated with starch levels present in leaves at the time of inoculation. In Chapter 6 it is shown that B. protearum exists as an endophyte in leaves of P. magnifica in naturally occurring as well as cultivated plants. In natural stands of proteas stem cankers are rare, but in cultivated plantations the incidence is high. Nutritional analyses indicate that higher levels of nitrogen occur in leaves of cultivated plants in spring, which could enhance disease development. High levels of sodium in the leaves of wild plants may restrict disease development. The severe economic losses caused by B. protearum make the search for improved methods of disease control essential. Fungicide applications form an important component of an integrated approach to disease management. In Chapter 7, in vitro tests demonstrate that tebuconazole, benomyl, prochloraz me, iprodione and fenarimol reduce the mycelial growth of B. protearum effectively. In the field there was a 25-85% reduction in the occurrence of stem cankers by applying fungicides or sanitation pruning. The best control was achieved by using benomyl, bitertanol, fenarimol, iprodione, prochloraz manganese chloride alternated with mancozeb and tebuconazole prophylactically. If sanitation pruning is combined with regular applications of fungicides, disease can be combated.
AFRIKAANSE OPSOMMING: Mikrofungi wat tot die genus Botryosphaeria behoort, is heterotrofiese organismes, wat patogenies op houtagtige plante kan wees. Hulle veroorsaak ernstige, en in sommige gevalle, verwoestende verliese, deur blaarnekrose, stamkankers en plantafsterwing. Die Proteaceae snyblom-industrie in Suid-Afrika maak 70% van die nasionale snyblomindustrie uit. Botryosphaeria siektes is 'n belangrike struikelblok in die produksie en handeldryf van Proteaceae, en daar is 'n ernstige behoefte om die etiologie, epidemiologie en beheer van siektes te ondersoek. Verliese van een van die belangrikste proteas, P. magnifica, beloop plaaslik 50% of meer. Die hoof doelstellings van hierdie studie was dus om die etiologie en epidemiologie van Botryosphaeria stamkankers op P. magnifica en ander Proteaceae vas te stel en metodes van siektebeheer te ondersoek. Hoewel daar 'n wye hoeveelheid inligting rakende die swam bestaan, wat in Hoofstuk I hersien is, is daar relatief min inligting oor Botryosphaeria op Proteaceae beskikbaar. Die taksonomie van Botryosphaeria benodig deeglike hersiening, en molekulêre tegnieke word benodig om spesie-identiteite op te klaar. In Hoofstuk 2 is gevind dat Phyllachora proteae, 'n blaarpatogeen van proteas, 'n Fusicoccum anamorf produseer, wat as F. proteae beskryf word. 'n Sphaeropsis-agtige synanamorf is met F. proteae geassosieer en 'n nuwe kombinasie vir P. proteae is as B. proteae in Botryosphaeria voorgestel. Die taksonomie van Botryosphaeria is, beide op die genus- as die spesievlak, in wanorde. In Hoofstuk 3 word die taksonomiese geskiedenis van Botryosphaeria hersien, en die genus word omskryf en van ander morfologies soortgelyke genera onderskei. Hoewel verskeie anamorf genera al met Botryosphaeria op grond van morfologiese waarnemings en filogenetiese analise van ITS rDNA volgorde data verbind is, word twee anamorf genera nou herken, dié met gepigmenteerde konidia (Diplodia), en dié met deurskynende konidia (Fusicoccum). Botryosphaeria proteae moet dus van Botryosphaeria uitgesluit word. Verskeie patogeniese Botryosphaeria spp. het 'n endofitiese fase in hul lewenssiklus. Hulle word dus onwetend in ander lande ingevoer waar hulle 'n gevaar vir landbou en inheemse plantegroei kan inhou. Die huidige wêreldverspreiding van Botryosphaeria spp. wat met Proteaceae geassosieer word is opgeklaar, en in Hoofstuk 4 word 'n sleutel tot die taksa wat met Proteaceae geassosieer word verskaf. Vyf Botryosphaeria spp. word met snyblom Proteaceae wêreldwyd geassosieer, naamlik B. lutea, B. protearum, B. proteae, B. ribis en B. obtusa. B. protearum word as 'n nuwe spesie beskryf. 'n Deeglike kennis van siekte-epidemiologie is noodsaaklik ten einde verliese te verminder. In Hoofstuk 5 dui ek aan dat letsels wat lei tot stamkankers, veroorsaak deur Botryosphaeria protearum op P. magnifica, in die hoofnerf of rant van blare ontstaan. Koch se postulate is uitgevoer en daar is vasgestel dat die aantal letsels wat vanuit kunsmatige inokulasies ontwikkel het korreleer met die styselvlakke teenwoordig in die blare ten tye van die inokulasie. In Hoofstuk 6 word getoon dat B. protearum as 'n endofiet in die blare van P. magnifica. In natuurlike standplase van proteas is stamkankers skaars, maar in verboude plantasies is die voorkoms hoog. Voedingsanalises dui aan dat hoër vlakke van stikstof in die blare van verboude plante in die lente voorkom, wat siekte-ontwikkeling moontlik kan bevorder. Hoë vlakke van natrium in die blare van natuurlike plante mag siekteontwikkeling beperk. Die ernstige ekonomiese verliese wat deur B. protearum veroorsaak word, maak die soektog na verbeterde metodes van siektebeheer noodsaaklik. Fungisiedtoedienings maak 'n belangrike deel uit van 'n geïntegreerde benadering tot siektebeheer. In Hoofstuk 7 dui in vitro toetse aan dat tebuconazole, benomyl, prochloraz me, iprodione en fenarimol die miseliumgroei van B. protearum effektief verminder. 'n Vermindering van 25-85% is aangetoon in die voorkoms van stamkankers in die veld, deur die toediening van fungisiedes en sanitasiesnoei. Die beste beheer is verkry deur die voorkomende toediening van benomyl, bitertanol, fenarimol, iprodione en prochloraz manganese chloride, afgewissel met mancozeb en tebuconazole, op plante in die veld. Indien sanitasiesnoei met gereelde toedienings van fungisiedes gekombineer word, kan die siekte bekamp word.

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Lubbe, Carolien M. „Colletotrichum diseases of Proteaceae“. Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53732.

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Thesis (MSc)--Stellenbosch University, 2004.
ENGLISH ABSTRACT: This thesis consists of four chapters that present research findings on Colletotrichum diseases associated with Proteaceae worldwide. The first chapter is a review of literature regarding the taxonomy and histology of Colletotrichum species associated with Proteaceae. The literature is not restricted to Proteaceae hosts, as information regarding Colletotrichum on Proteaceae is very limited. In chapter two, Colletotrichum spp. associated with proteaceous hosts growing in various parts of the world were identified based on morphology, sequence data of the internal transcribed spacer region (ITS-I, ITS-2), the 5.8S gene, and partial sequences of the B-tubulin gene. Four species of Colletotrichum were associated with Proteaceae. Colletotrichum gloeosporioides was isolated from Protea cynaroides cultivated in South Africa and Zimbabwe and from a Leucospermum sp. in Portugal, but is known to occur worldwide on numerous hosts. A recently described species, C. boninense, was associated with Zimbabwean and Australian Proteaceae, but also occurred on a Eucalyptus sp. in South Africa. This represents a major geographical and host extension for the species, and a description of the African strains is provided. Colletotrichum crassipes was represented by a single isolate obtained from a Dryandra plant in Madeira. Colletotrichum acutatum was isolated from Protea and Leucadendron in South Africa as well as from other proteaceous hosts occurring elsewhere. Colletotrichum acutatum f. sp. hakea was isolated from Hakea in South Africa. In chapter three, pathogenicity of these Colletotrichum species to certain proteas was established, relative aggressiveness of the different species tested and host response to them were compared as well as the effect that wounding had on host response. From the results obtained it is concluded that C. acutatum and C. gloeosporioides are the primary pathogens associated with Colletotrichum leaf necrosis, and C. acutatum is the main cause of anthracnose and stem necrosis of Proteaceae in South Africa. A histological study was performed in chapter four in response to the findings from the previous chapter. The behaviour of two C. acutatum isolates (one originating from Protea and the other from Hakea, C. acuataum f.sp. hakea) was studied on inoculated Protea leaf surfaces using light and scanning electron microscopy. Colletotrichum acutatum from Protea formed melanised appressoria on the leaf surface, whereas C. acutatum from Hakea formed very low numbers of both melanised and unmelanised appressoria. Most of the appressoria formed by C. acutatum from Protea were formed on the cell junctions and on the periclinal walls of the epidermal cells. From this study it is clear that C. acutatum f. sp. hakea is not a pathogen of Protea. Consequently the current use of this isolate as a biological control agent of Hakea in South Africa poses no threat to indigenous Protea species. Colletotrichum acutatum from Protea (although closely related to C. acutatum f. sp. hakea), is a pathogen of Protea, which was confirmed by histological observations. In conclusion, the present study has shown that several species of Colletotrichum are associated with diseased Proteaceae. These species differed in their pathogenicity and aggressiveness when inoculated onto certain protea cultivars. These differences could be partially explained by examining the behaviour of C. acutatum on the leaf surface. It is clear, however, that the distribution of the different species, their aggressrveness on different Proteaceae and their modes of infection needs to be investigated further. This work provides a basis for future research on the long-term effective management of these pathogens in fynbos production.
AFRIKAANSE OPSOMMING: Hierdie tesis bestaan uit vier hoofstukke wat handeloor navorsing van Colletotrichum siektes van Proteaceae wêreldwyd. Die eerste hoofstuk is 'n oorsig van literatuur rakende die taksonomie en histologie van die Colletotrichum spesies wat met Proteaceae geassosieer word. Die literatuur oorsig is nie beperk tot die Proteaceae nie aangesien baie min inligting rakende Colletotrichum op Proteaceae bestaan. In die tweede hoofstuk word die Colletotrichum spesies wat met proteas in verskeie dele van die wêreld geassosieer word, op grond van morfologie, DNS volgorde data van die interne getranskribeerde spasieerder area ("ITS-I, ITS-2"), die 5.8S geen, en gedeeltelike DNS volgordes van die B-tubulin geen geïdentifiseer. Vier Colletotrichum spesies is met die Proteaceae geassosieer. Colletotrichum gloeosporioides is geïsoleer vanaf Protea cynaroides wat in Suid-Afrika en Zimbabwe gekweek is en vanaf 'n Leucospermum sp. in Portugal, maar is bekend op verskeie gashere wêreldwyd. 'n Spesie wat onlangs beskryf is, C. boninense, is met Zimbabwiese en Australiaanse Proteaceae geassosieer, maar kom ook op 'n Eucalyptus sp. in Suid-Afrika voor. Dit is 'n groot uitbreiding van die geografiese voorkoms en gasheerreeks van hierdie spesie en 'n beskrywing van die Afrikaanse rasse word gegee. Colletotrichum crassipes is verteenwoordig deur 'n enkele isolaat wat vanaf 'n Dryandra plant in Madeira verkry is. Colletotrichum acutatum is vanaf Protea en Leucadendron in Suid-Afrika asook vanaf ander proteas wat elders voorkom, geïsoleer. Colletotrichum acutatum f. sp. hakea is vanaf Hakea in Suid-Afrika geïsoleer. In hoofstuk drie is die patogenisiteit van hierdie Colletotrichum spesies teenoor sekere proteas getoets, die relatiewe aggressiwiteit van die verskillende spesies is vergelyk, asook die gasheer se reaksie teenoor die spesies en die effek wat verwonding op die gasheer gehad het. Daar kan afgelei word vanaf die resultate dat C. acutatum en C. gloeosporioides die primêre patogene is wat met Colletotrichum blaarnekrose geassosieer word, en dat C. acutatum die hoof oorsaak is van antraknose en lootnekrose van Proteaceae in Suid-Afrika. 'n Histologiese studie is in hoofstuk vier uitgevoer in reaksie op die bevindings van die vorige hoofstuk. Die gedrag van twee C. acutatum isolate (een vanaf Protea en die ander vanaf Hakea, C. acutatum f.sp. hakea) op die oppervlaktes van geïnokuleerde Protea blare is bestudeer deur gebruik te maak van lig- en skandeer-elektronmikroskopie. Colletotrichum acutatum vanaf Protea vorm gemelaniseerde appressoria op die blaaroppervlak, terwyl C. acutatum vanaf Hakea klein hoeveelhede van beide gemelaniseerde en ongemelaniseerde appressoria vorm. Meeste van die appressoria wat deur C. acutatum vanaf Protea gevorm word, vorm op die aanhegtingspunte tussen selle en op die periklinale wande van die epidermale selle. Vanuit hierdie studie is dit duidelik dat C. acutatum f. sp. hakea nie 'n patogeen van Protea is nie. Gevolglik hou die huidige gebruik van hierdie isolaat as biologiese beheer agent van Hakea in Suid-Afrika geen gevaar in vir inheemse Protea spesies nie. Colletotrichum acutatum vanaf Protea (alhoewel dit naverwant is aan C. acutatum f. sp. hakea) is 'n patogeen van Protea en hierdie stelling is ook bevestig deur histologiese waarnemmgs. Ter samevatting het hierdie studie getoon dat verskeie Colletotrichum spesies geassosieer word met siektes van Proteaceae. Hierdie spesies het van mekaar verskil rakende patogenisiteit en aggressiwiteit nadat hulle op sekere protea kultivars geïnokuleer is. Hierdie verskille kon gedeeltelik verklaar word deur die gedrag van C. acutatum op die blaaroppervlaktes van verskillende protea kultivars. Dit is duidelik dat die verspreiding van die verskillende spesies, hulle aggressiwiteit op verskillende Proteaceae en hul infeksie metodes verder ondersoek moet word. Hierdie studie verskaf 'n basis vir toekomstige navorsing rakende lang-termyn effektiewe bestuur van hierdie patogene in fynbos aanplantings.

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Noronha, Christine M. (Christine Mary). „Effects of density and host plant type on fecundity and survival of Delia radicum (Bouché), D. Antiqua (Meigen) and D. Platura (Meigen) (Diptera: Anthomyiidae)“. Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41017.

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The effects of intraspecific and interspecific competition, host plant, and prior host plant experience on fecundity, rate of oviposition and mortality of adults and larval survival of Delia radicum Bouche (Cabbage Maggot (CM)), D. antiqua Meigen (Onion Maggot (OM)), and D. platura Meigen (Seed Corn Maggot (SCM)), were studied on cabbage, onion and bean plants.
An optimum density for maximum fecundity per female was observed when the four experimental densities were compared. This optimum density was higher on host than on non-host plants. CM females were host specific and did not oviposit on non-host plants. Rates of oviposition and mortality over a 30-day period were calculated for each density. The rate of oviposition was slower at higher densities on host plants for CM, OM and SCM. The rate of mortality increased at the highest density for CM (cabbage), OM (bean) and SCM (cabbage), but remained unaffected for OM on onion and cabbage and for SCM on onion and bean, when densities were compared. For OM, a delay in the rate of oviposition and mortality on cabbage (non-host plant) when compared with onion (host plant), suggests that cabbage was not as readily accepted as an oviposition site. Interspecific competition experiments at six density ratio's of SCM:OM indicated increased fecundity, or an increase in the rate of oviposition for OM, at the lower densities when single and mixed species were compared. For SCM no effects on fecundity were recorded, but the rate of oviposition was slower and rate of mortality faster at the lowest density in the presence of OM. Similar studies with SCM and CM showed no such effects of competition.
Host plant exposure of SCM females during the pre-oviposition period resulted in a delay in initial acceptance of subsequent host plants as oviposition sites. This happened only when females were exposed to a secondary host during the pre-oviposition period. Once oviposition began, host discrimination ceased and a switch in oviposition sites to the preferred host did not alter the rate of oviposition. In CM, the rate of larval development increased at density 6 (optimum density). Above this density a decrease in the rate of development and a significant reduction in pupal weight was observed. Time required for fly emergence was not affected by increasing larval densities.

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Wakgari, Waktola (Waktola Muleta). „Biology, ecology and management of white wax scale, Ceroplastes destructor Newstead (Hemiptera: Coccidae), on citrus and syzygium“. Thesis, Stellenbosch : Stellenbosch University, 2000. http://hdl.handle.net/10019.1/51637.

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Thesis (PhD)--University of Stellenbosch, 2000.
ENGLISH ABSTRACT: The population density of the white wax scale, Ceroplastes destructor Newstead, has increased since 1994 in certain areas of Western and parts of Eastern Cape Provinces of South Africa where citrus is grown, particularly on Citrus reticulata (Blanco). A study was conducted to investigate its morphology, biology and ecology as contributions to the development of a sound integrated management programme. Characteristics of the immature stages and adult females were described and illustrated from field-collected and slide-mounted specimens. A key to the different stages and morphometeric characteristics useful for separating them are provided. No significant differences in female fecundity were found between orchards (P > 0.05). However, fecundity varied significantly between female size classes from the same orchard (P < 0.001). Female body-size also differed significantly between orchards (P < 0.05) and was significantly positively correlated with fecundity (P < 0.01). C. destructor has one discrete generation per year in South Africa. Oviposition commenced in November and continued through to the end of December with a few females ovipositing until mid January. Population density of the second instar peaked in February while the third instar extended from March to the end of July, followed by a peak population of adults in August. Seven primary and three secondary parasitoids, as well as four predator species attacking C. destructor were identified. Aprostocetus (= Tetrastichus) ceroplastae (Girault) was the dominant species, accounting for 78.87% of the total primary parasitoids reared. Peak numbers of parasitoids and predators were synchronized with peak emergence of susceptible scale stages, indicating that the host-parasitoid/predator system contained a density-dependent regulatory mechanism. Key mortality factors varied slightly between two of the orchards. Key stage mortality determined from a cohort life table was generally in the third instar (LIlI) and preovipositional female (POF) stage. Significant density-dependent mortality factors were demonstrated for the first instar (LI) and PDF stage. Dispersal of C. destructor is by first instar nymphs and the numbers caught on a series of yellow sticky traps varied significantly between crawler densities at the source, trap distances and trap directions from the source (P < 0.001). The numbers caught were positively correlated to the initial crawler density at the source (P < 0.01), suggesting that dispersal was density dependent. Trap distance and the numbers caught were inversely correlated (P < 0.01). Evaluation of effects of different densities of C. destructor on growth, survivorship and reproduction of scales as well as on leaf bearing ability of trees and area of leaf surface covered with sooty mould fungus was carried out on naturally infested Syzygium (= Eugenia) malaccensis (L.) plants. Scale body size and fecundity were inversely related to scale density (P < 0.01), suggesting density-dependent intraspecific competition. Scale survivorship generally declined with increasing density whereas scale parasitism and predation were positively correlated with density (P < 0.05). At high scale densities production of new leaves was significantly reduced (P < 0.01), reducing the resource base for subsequent generations of scale. Scale density and leaf area covered with sooty mould fungus were significantly positively correlated (P < 0.05). The toxicity of four synthetic insecticides against the three immature stages of C. destructor and of eight insecticides against the parasitoid A. ceroplastae was evaluated. Development of the first and second instars of C. destructor was completely arrested by the chemicals. Female fecundity, fertility and body sizes of survivors of treatments applied at the LIII stage were not significantly affected by any of the chemicals (P > 0.05). All the chemicals exhibited high toxicity to A. ceroplastae and hence are not recommended for integrated management of C. destructor in citrus orchards where A. ceroplastae plays an important role.
AFRIKAANSE OPSOMMING: Die populasiedigtheid van die witwasdopluis, Ceroplastes destructor Newstead, het sedert 1994 toegeneem in sekere gebiede van die Weskaap en Ooskaap provinsies van Suid-Afrika waar sitrus verbou word, veralop Citrus reticulata (Blanco). 'n Studie van hierdie insek se morfologie, biologie en ekologie is onderneem as bydrae tot die ontwikkeling van 'n geïntegreerde bestuursprogram. Die karaktertrekke van die onvolwasse stadia en die volwasse wyfies is beskryf en geïllustreer vanaf eksemplare wat in die veld versamel is en op g1asplaatjies gemonteer is. 'n Sleutel vir die verskillende stadia en morfometriese kenmerke wat nuttig is om hulle te onderskei, word voorsien. Geen beduidende verskille in die vrugbaarheid van wyfies van verskillende boorde is gevind nie (P < 0.05). Vrugbaarheid het egter betekenisvol verskil by die verskillende grootteklasse van wyfies uit dieselfde boord (P < 0.001). Die liggaamsgrootte van wyfies uit verskillende boorde het betekenisvol verskil (P < 0.05) en was betekenisvol positief gekorreleer met vrugbaarheid (P < 0.01). C. destructor het een generasie per jaar in Suid-Afrika. Eierlegging het in November begin en aangehou tot aan die einde van Desember, met enkele wyfies wat nog tot in middel Januarie eiers gelê het. Die populasiedigtheid van die tweede instar het 'n hoogtepunt in Februarie bereik, terwyl die derde instar van Maart tot aan die einde van Julie geduur het, gevolg deur 'n piekbevolking van volwassenes in Augustus. Sewe primêre en drie sekondêre parasitoïde asook vier predator spesies wat C destructor aanval, is geïdentifiseer. Aprostocetus (=Tetrastichus) ceroplastae (Girault) was die dominante spesies wat 78.87% van die totale aantal primêre parasitoïde wat uitgeteel is, uitgemaak het. Die pieke in die getalle van parasitoïde en predatore was gesinchroniseer met pieke in die verskyning van die gevoelige stadia, wat dui op die aanwesigheid van 'n digtheidsafhanklike regulatoriese meganisme. Die sleutel mortaliteitsfaktore het effens gevarieer tussen twee van die boorde. Die sleutelstadium van mortaliteit, soos bepaal m.b.v. 'n kohort lewenstabel, was gewoonlik die derde instar (LIlI) en die preoviposisionele wyfie (POW). Betekenisvolle digtheidsafhanklike mortaliteitsfaktore IS aangetoon vir die eerste instar (LI) en die POW. Die verspreiding van C.destructor vind plaas deur die eerste instar nimfe en die getalle wat op 'n reeks van taai geel valle gevang is, het betekenisvol gewissel volgens kruiperdigthede by die bron, asook die afstand en rigting van die valle vanaf die bron (P < 0.001). Die getalle wat gevang is, was positief gekorreleer met die aanvanklike kruiperdigtheid by die bron (P < 0.01), wat daarop dui dat verspreiding digtheidsafhanklik was. Die afstand van die valle en die aantal wat gevang is, was omgekeerd gekorreleer (P < 0.01). 'n Evaluering van die invloed van verskillende digthede van C. destructor op die groei, oorlewing en reproduksie van dopluise, asook die vermoë van bome om blare te dra en die area van die blaaroppervlak wat met roetskimmel besmet is, is uitgevoer op plante van Syzygium (= Eugenia) malaccensis (L.) met 'n natuurlike besmetting. Die liggaamsgrootte en vrugbaarheid van die dopluise was omgekeerd gekorreleer met hulle digtheid (P < 0.01), wat dui op digtheidsafhanklike intraspesifieke kompetisie. Die oorlewing van die dopluise het oor die algemeen afgeneem met toenemende digtheid, terwyl parasitisme en predasie positief gekorreleer was met digtheid (P < 0.05). By hoë dopluisdigthede het die produksie van nuwe blare betekenisvol afgeneem (P < 0.01), wat die hulpbronbasis vir daaropvolgende generasies van dopluise verswak. Die dopluisdigtheid en blaaroppervlak wat met roetskimmel bedek was, was positief gekorreleer (P < 0.05). Die toksisiteit van vier sintetiese insektemiddels teenoor die drie onvolwasse stadia van C. destructor en van agt insektemiddels teenoor die parasitoïd A. ceroplastae is geëvalueer. Die ontwikkeling van die eerste en tweede instars van C. destructor is heeltemal stopgesit deur die middels. Die fekunditeit, fertiliteit en liggaamsgrootte van wyfies wat toedienings op die LIIl stadium oorleef het, is nie betekenisvol ge-affekteer deur enige van die middels nie (P < 0.05). Al die middels was baie toksies teenoor A. ceroplastae en word dus nie aanbeveel vir die geïntegreerde bestuur van C. destructor waar A. ceroplastae 'n belangrike rol speel nie.

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Rodriguez, Juan Jose. „Movement and Accumulation of Candidatus Liberibacter Solanacearum in Potato Plants“. Diss., North Dakota State University, 2012. https://hdl.handle.net/10365/26726.

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A new disease affecting potatoes was first detected in Mexico in 1993. Affected plants had aerial symptoms similar to those caused by potato purple top and psyllid yellows, but tubers had internal brown discoloration when sliced and dark stripes and streaks when processed to produce potato chips. The disease has been found in many potato production areas in Guatemala, Mexico, Honduras, New Zealand and the United States. The disease, termed Zebra Chip (ZC), has been associated with the presence of heavy infestations of the potato-tomato psyllid (Bactericera cockerelli). In 2009, a research group in New Zealand discovered that a new disease in tomato and pepper plants was caused by Candidatus Liberibacter solanacearum (Lso) and subsequently this same bacterium was associated with ZC in potato samples from Texas. The objectives of this study were: to assess the accumulation of Lso in various potato organs, to determine the effect of plant age on detection of Lso, symptom development and plant death, and (iii) to determine the effect of phosphorous acid on the development of ZC. Results from these studies showed significant differences in Lso populations between above and below ground tissues of the potato plant, with Lso populations in stolons and tubers being three to four times higher than those of leaf tissue and over seventy times greater than in stems. Time for detection of Lso by PCR in potato leaves of different ages at the time of inoculation ranged from 21 to 26 days after inoculation, symptoms development took 23 to 36 days. Plant death, took 24 to 47 days in plants of different age groups at the time of inoculation. In plants 15 weeks old at the time of inoculation, Lso was detected after 14 days in one plant out of 18; in plants 16 weeks old at the time of inoculation, Lso was detected after seven days in two plants out of 18. Phosphorous acid applications had no effect on the populations of Lso in potato tubers, onset of symptoms or plant death. All tubers showed ZC symptoms, making them unacceptable for the market.
North Dakota State University. Department of Plant Pathology

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Kawchuk, Lawrence Michael. „Molecular characterization of potato leafroll luteovirus and development of genetically engineered resistance“. Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30684.

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Complementary DNA (cDNA) clones representing approximately 5800 nucleotides of potato leafroll virus (PLRV) genomic RNA were generated, restriction-mapped, and partially sequenced. Within one of the cDNA clones an open reading frame (ORF) that could encode a 23 kDa protein was identified and further characterized. Comparison of the deduced amino acid sequence with the coat protein amino acid sequence of the PAV strain of barley yellow dwarf luteovirus (BYDV-PAV) showed significant similarity. This observation together with its size and internal location within the genome suggested that this gene encoded the PLRV coat protein. Other similarities were observed between PLRV and BYDV sequences in this region of their genomes, including a 17 kDa ORF within the ORF encoding the 23 kDa coat protein, and termination of the latter with an amber codon which is immediately followed by a large ORF in the same reading frame. Three PLRV coat protein gene sequences were used to transform tobacco and the potato cultivars 'Desiree' and 'Russet Burbank' via Agrobacterium tumefaciens mediated gene transfers. One construct possessed 12 nucleotides of the untranslated leader sequence 5' to the putative coat protein gene start codon. The other construct, which was also inserted in the reverse orientation to produce negative-sense RNA, had 192 nucleotides from this leader sequence. When these sequences were introduced as chimaeric genes under the control of a duplicated cauliflower mosaic virus 35S (CaMV) promoter, transcription levels were high. Both positive-sense transcripts produced potato leafroll coat protein which accumulated to maximum levels of approximately 0.5% and 0.01% of total leaf protein in tobacco and potato, respectively. Results show that significant levels of inoculum concentration-independent sustained resistance were obtained with each construct, resulting in PLRV titres as low as 1% of the level observed in untransformed plants, as determined by enzyme-linked immunosorbent assays. Virus transmission from PLRV-inoculated transgenic 'Russet Burbank' was reduced substantially and was correlated with virus titre. The pattern and level of protection were the same for constructs producing positive- and negative-sense RNA, suggesting a similar mechanism of resistance. Virus levels were negatively correlated to transcript levels within the transgenic plants. This resistance will have practical applications for the control, of PLRV. Elucidation of the mechanism of resistance may also help understand the mechanisms of virus infection.
Land and Food Systems, Faculty of
Graduate

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Bücher zum Thema "Diseases and pests"

Books, Time-Life, Hrsg. Pests & Diseases. Alexandria, Va: Time-Life Books, 1995.

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Andrew, Halstead, und Royal Horticultural Society (Great Britain), Hrsg. Pests & diseases. London: Dorling Kindersley, 1997.

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Andrew, Halstead, und Royal Horticultural Society, Hrsg. Garden pests & diseases. London: Mitchell Beazley, 1992.

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Society, American Horticultural, Hrsg. Pests and diseases. New York: Dorling Kindersley, 2000.

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Books, Sunset, Hrsg. Garden pests & diseases. Menlo Park, Calif: Sunset Pub. Corp., 1993.

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Beatrice, Henricot, Hrsg. Pests and diseases. New York: DK Publishing, 2010.

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Sumner, Daniel A., Hrsg. Exotic Pests and Diseases. Ames, Iowa, USA: Blackwell Publishing Company, 2003. http://dx.doi.org/10.1002/9780470290125.

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Gair, R. Cereal pests and diseases. 4. Aufl. Ipswich, [England]: Farming Press, 1987.

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Sathe, T. V. Sugarcane pests and diseases. Delhi, India: Manglam Publications, 2009.

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Society, American Orchid, Hrsg. Orchid pests and diseases. West Palm Beach, Fla. (6000 S. Olive Ave., West Palm Beach 33405): The Society, 1995.

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Buchteile zum Thema "Diseases and pests"

Thompson, Anthony Keith, und Ibok Oduro. „Diseases and pests.“ In Yams: botany, production and uses, 76–90. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789249279.0006.

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Abstract This chapter describes the different diseases and pests that affect yams and various methods for their control. It particularly covers the following aspects: field fungal diseases; postharvest fungal diseases; specific fungal diseases; control of fungal diseases; bacterial diseases; viral diseases; physiological disorders; insect pests; and nematode pests.

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Laksono, N. D., U. Setiawati, F. Nur, M. Rahmaningsih, Y. Anwar, H. Rusfiandi, B. P. Forster, E. H. Sembiring, A. S. Subbarao und H. Zahara. „Pests and diseases.“ In Nursery practices in oil palm: a manual, 77–85. Wallingford: CABI, 2019. http://dx.doi.org/10.1079/9781789242140.0077.

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Tayal, Mukesh K., und T. P. S. Chauhan. „Silkworm Diseases and Pests“. In Industrial Entomology, 265–89. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3304-9_9.

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Hunsigi, Gururaj. „Weeds, Pests and Diseases“. In Advanced Series in Agricultural Sciences, 144–56. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-78133-9_8.

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Lebot, V. „Cassava: pests and diseases.“ In Tropical root and tuber crops: cassava, sweet potato, yams and aroids, 73–88. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243369.0073.

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Lebot, V. „Yams: pests and diseases.“ In Tropical root and tuber crops: cassava, sweet potato, yams and aroids, 293–307. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243369.0293.

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Lebot, V. „Aroids: pests and diseases.“ In Tropical root and tuber crops: cassava, sweet potato, yams and aroids, 406–19. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243369.0406.

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Sipes, B., und A. P. de Matos. „Pests, diseases and weeds.“ In The pineapple: botany, production and uses, 269–94. Wallingford: CABI, 2018. http://dx.doi.org/10.1079/9781786393302.0269.

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Thompson, A. K., R. K. Prange, R. D. Bancroft und T. Puttongsiri. „Pests, diseases and disorders.“ In Controlled atmosphere storage of fruit and vegetables, 64–74. Wallingford: CABI, 2018. http://dx.doi.org/10.1079/9781786393739.0064.

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Sipes, Brent, und Koon-Hui Wang. „Pests, diseases and weeds“. In Handbook of Pineapple Technology, 62–88. Chichester, UK: John Wiley & Sons, Ltd, 2016. http://dx.doi.org/10.1002/9781118967355.ch4.

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Konferenzberichte zum Thema "Diseases and pests"

Lisboa, Eduardo, Givanildo Lima und Fabiane Queiroz. „Coffee Leaf Diseases Identification and Severity Classification using Deep Learning“. In Anais Estendidos da Conference on Graphics, Patterns and Images. Sociedade Brasileira de Computação - SBC, 2021. http://dx.doi.org/10.5753/sibgrapi.est.2021.20039.

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In this paper, we propose a method for automatic identification and classification of leaf diseases and pests in the Brazilian Arabica Coffee leaves. We developed a Machine Learning model, trained with the BRACOL public image dataset, to evaluate if a given image of a leaf has a disease or pest — Miner, Phoma, Cercospora and Rust — or if it is healthy. We then compared our model with other famous and well-known classification models, and we were able to achieve an accuracy of 98,04%, which greatly exceeds the accuracy of the other methods implemented. In addition, we developed an assessment to perform a classification related to the percentage of each leaf that is affected by the disease, achieving an accuracy of approximately 90%.

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Munkvold, Gary P. „Managing Diseases and Pests with Seed Treatments“. In Proceedings of the 16th Annual Integrated Crop Management Conference. Iowa State University, Digital Press, 2007. http://dx.doi.org/10.31274/icm-180809-893.

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Tumang, Gina S. „Pests and Diseases Identification in Mango using MATLAB“. In 2019 5th International conference on Engineering, Applied Sciences and Technology (ICEAST). IEEE, 2019. http://dx.doi.org/10.1109/iceast.2019.8802579.

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Deepa, A. R., Mousmi Ajay Chaurasia, S. Bala Naga Vamsi, B. Manoj Kumar, V. Srinnivas Reddy und K. Trilok Anand. „Plant Diseases and Pests Detection Using Machine Learning“. In 2023 3rd Asian Conference on Innovation in Technology (ASIANCON). IEEE, 2023. http://dx.doi.org/10.1109/asiancon58793.2023.10270264.

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Kulikov, M. A., A. N. Kulikova und A. V. Goncharov. „Resistance of sunflower hybrids to herbicides, diseases, pests and weeds“. In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-158.

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Wang, Qiyao, Guiqing He, Feng Li und Haixi Zhang. „A novel database for plant diseases and pests classification“. In 2020 IEEE International Conference on Signal Processing, Communications and Computing (ICSPCC). IEEE, 2020. http://dx.doi.org/10.1109/icspcc50002.2020.9259502.

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Patel, Pruthvi P., und Dineshkumar B. Vaghela. „Crop Diseases and Pests Detection Using Convolutional Neural Network“. In 2019 IEEE International Conference on Electrical, Computer and Communication Technologies (ICECCT). IEEE, 2019. http://dx.doi.org/10.1109/icecct.2019.8869510.

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Min Htet, Aung Si, und Hyo Jong Lee. „Strawberry Pests and Diseases Recognition with Self-Supervised Learning“. In 2023 Congress in Computer Science, Computer Engineering, & Applied Computing (CSCE). IEEE, 2023. http://dx.doi.org/10.1109/csce60160.2023.00321.

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„Identifying tomato diseases and pests using intelligent machine vision“. In Data-Driven Agriculture in the Asian and Pacific Region – To Ensure Productivity and Sustainability. Food and Fertilizer Technology Center for the Asian and Pacific Region, 2021. http://dx.doi.org/10.56669/qptp5112.

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Alfarisy, Ahmad Arib, Quan Chen und Minyi Guo. „Deep learning based classification for paddy pests & diseases recognition“. In ICMAI '18: 2018 International Conference on Mathematics and Artificial Intelligence. New York, NY, USA: ACM, 2018. http://dx.doi.org/10.1145/3208788.3208795.

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Berichte der Organisationen zum Thema "Diseases and pests"

Solomon, J. D., T. D. Leininger, A. D. Wilson, R. L. Anderson, L. C. Thompson und F. I. McCracken. Ash pests: a guide to major insects, diseases, air pollution injury, and chemical injury. New Orleans, LA: U.S. Department of Agriculture, Forest Service, Southern Forest Experiment Station, 1993. http://dx.doi.org/10.2737/so-gtr-096.

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Amanor, Kojo, Joseph Yaro, Joseph Teye und Steve Wiggin. Ghana’s Cocoa Farmers Need to Change Gear: What Policymakers Need to Know, and What They Might Do. Institute of Development Studies (IDS), März 2022. http://dx.doi.org/10.19088/apra.2022.008.

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Cocoa farmers in Ghana face increasing challenges. In the past, many of them could make a living from cocoa thanks to the advantages – ‘forest rents’ – that initially apply when forest is cleared to create cocoa farms: fertile soils, few pests and diseases. With time, however, weeds invade, pests and diseases build up, and trees age. To maintain production requires more labour, more inputs and more skill. In the past, farmers would often abandon older groves and seek new forest to clear. As they did so, the frontier for cocoa farming moved westwards across Ghana to the remaining high forest. But by 2000 or so, no new forest was available. Farmers now have to manage aging stands of trees, clear weeds and parasites, and combat pests, fungi and diseases. In Suhum District in the east and in Juaboso District in the far west of Ghana, we talked to farmers. They understood the challenges they faced, and knew how to deal with some of them. But many were not farming their cocoa as well as they could, losing yields and income as a result. This brief provides a basis for policymakers to move forward in responding to the current challenges facing cocoa farmers.

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Kosiba, Alexandra, Emma Tait, Gene Desideraggio, Alyx Belisle, Clarke Cooper und James Duncan. Threats to the Urban Forest: The potential economic impacts of invasive forest pests and diseases in the Northeast. Forest Ecosystem Monitoring Cooperative, Juni 2020. http://dx.doi.org/10.18125/8w9j42.

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Davis, Cristina, Amots Hetzroni, Alexander Aksenov, Michael J. Delwiche, Victoria Soroker und Victor Alchanatis. Development of a universal volatile compound detection technology for early recognition of pests and diseases in fruit trees. United States Department of Agriculture, Januar 2015. http://dx.doi.org/10.32747/2015.7600016.bard.

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Tzin, V., B. Dilkes und H. Sela. Identifying molecular markers for defense metabolites against aphid feeding in wild emmer wheat. Israel: United States-Israel Binational Agricultural Research and Development Fund, 2021. http://dx.doi.org/10.32747/2021.8134174.bard.

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Bread wheat is the second most important food crop, providing calories to half of the world population and roughly one-fifth of the calories and protein consumed by people worldwide. Crop losses to insect pests depress yields and climate change is expected to significantly increase this problem. To combat global food insecurity, the protection of crops from insect pests must be prioritized. The Bird cherry-oat aphid, Rhopalosiphum padi, is the most abundant and economically important cereal pest, causes up to 40-60 % yield loss through direct feeding and vectoring plant diseases. The main goal of this project was to identify molecular markers associated with aphid resistance and defense metabolite levels in wild emmer wheat (WEW). During the three years of this project, we screened a large number of WEW accessions and conducted a genome-wide association study (GWAS) analysis. We experimented with data processing and explored different GWAS models, which revealed a massive number of determinants of aphid resistance and highlighted hundreds of single nucleotide polymorphisms (SNPs). Thus, we focused on a small subset of genes that linked to the most significant SNPs. By the end of this collaboration, we have a list of important SNPs, and three major genes that we believe have a strong effect on wheat resistance to aphids. Work on these genes is continuing and will soon publish our exciting new results.

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Audsley, Neil, Gonzalo Avila, Claudio Ioratti, Valerie Caron, Chiara Ferracini, Tibor Bukovinszki, Marc Kenis et al. Pine Processionary Moth, Thaumetopoea pityocampa (Denis & SchiffermÃ¼ller). Euphresco, 2023. http://dx.doi.org/10.1079/20240228697.

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The pine processionary moth (Thaumetopoea pityocampa; PPM) is native to southern Europe, North Africa and parts of the Middle East, but has expanded northwards into several European countries. The larvae feed on conifer needles, causing severe defoliation and weakening trees, making them more susceptible to other pests and diseases. The urticating setae of older larvae pose significant health risks to humans and animals, causing skin and respiratory issues. While no classical biological control programs specifically target PPM, several natural enemies show promise. Key parasitoids include the egg parasitoids Baryscapus servadeii and Ooencyrtus pityocampae, the larval parasitoid Phryxe caudata and the pupal parasitoids Villa brunnea and Coelichneumon rudis. These parasitoids exhibit variable parasitism rates depending on region and conditions. Additionally, the ground beetle Calosoma sycophanta has been used in augmentative releases for control, demonstrating potential for classical biological control in areas where it does not naturally exist.

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Tait, Susan M., Charles G. III Shaw und Andris Eglitis. Occurrence of insect and disease pests on young-growth Sitka spruce and western hemlock in southeastern Alaska. Portland, OR: U.S. Department of Agriculture, Forest Service, Pacific Northwest Forest and Range Experiment Station, 1985. http://dx.doi.org/10.2737/pnw-rn-433.

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Morin, S., L. L. Walling, Peter W. Atkinson, J. Li und B. E. Tabashnik. ets for CRISPR/Cas9-mediated gene drive in Bemisia tabaci. Israel: United States-Israel Binational Agricultural Research and Development Fund, 2021. http://dx.doi.org/10.32747/2021.8134170.bard.

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The goal of our BARD proposal was to build both the necessary infrastructure and knowledge for using the CRISPR/Cas9-based gene drive system to control the whitefly Bemisia tabaci. Our research focused on achieving three main goals: (1) establishing a CRISPR/Cas9 gene-editing system for producing genetically-edited B. tabaci; (2) generating and testing CRISPR/Cas9-mediated mutations targeting genes that represent two gene drive strategies: population replacement and population suppression; (3) using computer modeling to optimize strategies for applying CRISPR/Cas9 to control B. tabaci populations in the field. CRISPR gene drive is one of the most promising strategies for diminishing the negative impacts of harmful insects. This technique can introduce mutations into wild populations of pests that reduce their ability to cause damage, reduce their population size, or both. In principle, this can be selfsustaining because mutations carried by relatively few insects can increase in frequency and spread quickly throughout wild populations. Because of this sustainability and the potential benefits to society, agricultural gene-drive systems are most likely to be funded by government agencies, foundations, and grower associations; as with sterile insect releases and most biocontrol programs. Although gene drives have received intensive study in Drosophila and mosquito vectors of human disease, we were one of the first teams pursuing this approach for crop pests. Our project was also one of the first to address CRISPR gene drive in the Hemiptera, an insect order that includes hundreds of pest species. We focused on developing and implementing CRISPR gene drive to reduce the massive damage caused by B. tabaci. This haplodiploid insect is one of the world's most devastating crop pests. Whereas extensive work by others explored CRISPR in diploid species, our project pioneered application of this revolutionary technology to haplodiploids, which have a distinct system of inheritance that presents special challenges and opportunities. Our project has achieved several breakthroughs, including publication of the first paper analyzing CRISPR gene drive in haplodiploids (Li et al. 2020, see next section). Our modeling results from this landmark study demonstrate that CRISPR gene drive can work in haplodiploids, especially if fitness costs associated with the driver allele are low or nil. Our paper was the first to provide a conceptual framework for evaluating and optimizing CRISPR gene drive strategies for managing B. tabaci and other haplodiploid pests. Our breakthroughs in the laboratory have created the infrastructure needed to develop CRISPR for controlling B. tabaci. We established a microinjection system enabling us to introduce CRISPR-derived mutations into B. tabaci embryos. We have used this system to generate and track inherited eye-color mutants of B. tabaci. We have identified and cloned germline promoters, and demonstrated their function in transgenic B. tabaci embryos and other hemipteran insects. We have also developed a tool to easily identify B. tabaci harboring CRISPR-mediated mutations by tagging target genes using a transgenic fluorescent marker. The successful completion of our project provides all the knowledge and infrastructure essential for developing a novel genetic approach for B. tabaci control, which can serve as a non-chemical "green" alternative for managing this global pest. We predict that our discoveries will accelerate the development of the CRISPR gene drive technique for reducing the numbers of this pest and the damage it causes. Still, realization of the benefits of gene-drive technology for pest control will require sustained attention to potential environmental and societal impacts, as well as regulatory and implementation challenges. Given the great promise of this technology and the urgent need for better control methods, we expect that guidance documents and regulations will be in place to allow the scientific community to safely move gene drives for pest control from the laboratory to field trials.

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Mueller, Alexander, Elena Lazutkaite, Adam Prakash, Mark Davis, Ahmed Amdihun und Jully Ouma. Scientific Linkages Between Climate Change and (Transboundary) Crop Pest and Disease Outbreaks. TMG Research gGmbH, August 2022. http://dx.doi.org/10.35435/2.2022.5.

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