Thematische Bibliographien / Decellularized matrix

Auswahl der wissenschaftlichen Literatur zum Thema „Decellularized matrix“

Autor: Grafiati
Veröffentlicht am 25. Mai 2024

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Zeitschriftenartikel zum Thema "Decellularized matrix"

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Hashemi, Javad, Ghasem Barati und Bahram Bibak. „Decellularized Matrix Bioscaffolds“. Pancreas 50, Nr. 7 (August 2021): 942–51. http://dx.doi.org/10.1097/mpa.0000000000001868.

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Hashemi, Javad, Ghasem Barati und Bahram Bibak. „Decellularized Matrix Bioscaffolds“. Pancreas 50, Nr. 7 (August 2021): 942–51. http://dx.doi.org/10.1097/mpa.0000000000001868.

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Nakamura, Naoko, Ai Ito, Tsuyoshi Kimura und Akio Kishida. „Extracellular Matrix Induces Periodontal Ligament Reconstruction In Vivo“. International Journal of Molecular Sciences 20, Nr. 13 (03.07.2019): 3277. http://dx.doi.org/10.3390/ijms20133277.

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One of the problems in dental implant treatment is the lack of periodontal ligament (PDL), which supports teeth, prevents infection, and transduces sensations such as chewiness. The objective of the present study was to develop a decellularized PDL for supporting an artificial tooth. To this end, we prepared mouse decellularized mandible bone with a PDL matrix by high hydrostatic pressure and DNase and detergent treatments and evaluated its reconstruction in vivo. After tooth extraction, the decellularized mandible bone with PDL matrix was implanted under the subrenal capsule in rat and observed that host cells migrated into the matrix and oriented along the PDL collagen fibers. The extracted decellularized tooth and de- and re-calcified teeth, which was used as an artificial tooth model, were re-inserted into the decellularized mandible bone and implanted under the subrenal capsule in rat. The reconstructed PDL matrix for the extracted decellularized tooth resembled the decellularized mandible bone without tooth extraction. This demonstrates that decellularized PDL matrix can reconstruct PDL tissue by controlling host cell migration, which could serve as a novel periodontal treatment approach.
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Meyer, Tanja, Serghei Cebotari, Gudrun Brandes, Dagmar Hartung, Frank Wacker, Monika Theis, Tim Kaufeld et al. „Decellularized Porcine Pericardium Enhances Autologous Vascularized Matrix as a Prosthesis for Left Ventricular Full-Wall Myocardial Reconstruction“. Prosthesis 5, Nr. 1 (01.02.2023): 113–29. http://dx.doi.org/10.3390/prosthesis5010010.

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Regenerative grafts for myocardial reconstruction are often mechanically not stable enough to withstand the left ventricle’s high blood pressure. Hence, decellularized pericardium may serve as a stabilizing structure for biological myocardium prostheses. The efficacy of detergent- and enzyme-based protocols to decellularize porcine pericardium was compared. Then, the decellularized pericardium was employed for a primary cover of a transmural left ventricular defect in minipigs (n = 9). This pericardium patch was applied to mitigate the high-pressure load on an autologous stomach tissue, which was utilized as a regenerative tissue prosthesis. Decellularization of the porcine pericardium with deoxycholic acid (DOA)- and enzyme-based protocols (trypsin/EDTA) removed 90% of the original cells (p < 0.001). The trypsin/EDTA protocol significantly altered the matrix architecture compared to the DOA protocol. There were no infections or clinical signs of graft rejection following the transplantation of the decellularized pericardium and the autologous segment of the stomach in the surviving animals (n = 7). A good left ventricular function could be detected via MRI six months following surgery. The biological integration of the graft into the host’s tissue was found histologically. The stabilization of initially fragile grafts with decellularized pericardium facilitates the application of regenerative myocardial prostheses even on the left ventricle.
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Belviso, Immacolata, Anna Maria Sacco, Domenico Cozzolino, Daria Nurzynska, Franca Di Meglio, Clotilde Castaldo und Veronica Romano. „Cardiac-derived extracellular matrix: A decellularization protocol for heart regeneration“. PLOS ONE 17, Nr. 10 (19.10.2022): e0276224. http://dx.doi.org/10.1371/journal.pone.0276224.

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Extracellular matrix (ECM) is a fundamental component of the heart, guiding vital cellular processes during organ homeostasis. Most cardiovascular diseases lead to a remarkable remodeling of the ECM, accompanied by the formation of a fibrotic tissue that heavily compromises the heart function. Effective therapies for managing fibrosis and promoting physiological ECM repair are not yet available. The production of a decellularized extracellular matrix (d-ECM) serving as a three-dimensional and bioactive scaffold able to modulate cellular behavior and activities is considered crucial to achieve a successful regeneration. The protocol represents a step-by-step method to obtain a decellularized cardiac matrix through the combination of sodium dodecyl sulphate (SDS) and Triton X-100. Briefly, cardiac samples obtained from left ventricles of explanted, pathological human hearts were dissected and washed to remove residual body fluids. Samples were then snap-frozen and sliced by a cryostat into 350 μm thick sections. The sections obtained were decellularized using a solution containing 1% Triton X-100 and 1% SDS in combination, for 24 hours, until observing the color change from brownish-red to translucent-white. As a result, the protocol shows efficiency in preserving ECM architecture and protein composition during the whole process, suggesting that it is worthwhile, highly reproducible and produces a well- preserved decellularized extracellular matrix from cardiac samples. Notwithstanding, some limitations need to be addressed, such as the risk for microbial contamination and the unpredictable trend of the protocol when applied to decellularize samples other than myocardium, vessels, or skin. These issues require antibiotics mixture supplement during the procedure followed by UV sterilization, and appropriate adjustments for a tissue-specific utilization, respectively. The protocol is intended to produce a cardiac d-ECM for cell settlement, representing the ideal scaffold for tissue engineering purposes.
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Wu, Jinglei, Jiazhu Xu, Yihui Huang, Liping Tang und Yi Hong. „Regional-specific meniscal extracellular matrix hydrogels and their effects on cell–matrix interactions of fibrochondrocytes“. Biomedical Materials 17, Nr. 1 (23.12.2021): 014105. http://dx.doi.org/10.1088/1748-605x/ac4178.

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Abstract Decellularized meniscal extracellular matrix (ECM) material holds great potential for meniscus repair and regeneration. Particularly, injectable ECM hydrogel is highly desirable for the minimally invasive treatment of irregularly shaped defects. Although regional-specific variations of the meniscus are well documented, no ECM hydrogel has been reported to simulate zonally specific microenvironments of the native meniscus. To fill the gap, different (outer, middle, and inner) zones of porcine menisci were separately decellularized. Then the regionally decellularized meniscal ECMs were solubilized by pepsin digestion, neutralized, and then form injectable hydrogels. The hydrogels were characterized in gelation behaviors and mechanical properties and seeded with bovine fibrochondrocytes to evaluate the regionally biochemical effects on the cell–matrix interactions. Our results showed that the decellularized inner meniscal ECM (IM) contained the greatest glycosaminoglycan (GAG) content and the least collagen content compared with the decellularized outer meniscal ECM (OM) and middle meniscal ECM (MM). The IM hydrogel showed lower compressive strength than the OM hydrogel. When encapsulated with fibrochondrocytes, the IM hydrogel accumulated more GAG, contracted to a greater extent and reached higher compressive strength than that of the OM hydrogel at 28 days. Our findings demonstrate that the regionally specific meniscal ECMs present biochemical variation and show various effects on the cell behaviors, thus providing information on how meniscal ECM hydrogels may be utilized to reconstruct the microenvironments of the native meniscus.
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Ling, You, Weikang Xu, Lifeng Yang, Changyan Liang und Bin Xu. „Improved the biocompatibility of cancellous bone with compound physicochemical decellularization process“. Regenerative Biomaterials 7, Nr. 5 (30.08.2020): 443–51. http://dx.doi.org/10.1093/rb/rbaa024.

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Abstract Due to the unique microstructures and components of extracellular matrix (ECM), decellularized scaffolds had been used widely in clinical. The reaction of the host toward decellularized scaffolds depends on their biocompatibility, which should be satisfied before applied in clinical. The aim of this study is to develop a decellularized xenograft material with good biocompatibility for further bone repair, in an effective and gentle method. The existing chemical and physical decellularization techniques including ethylene diamine tetraacetic acid (EDTA), sodium dodecyl sulfate (SDS) and supercritical carbon dioxide (SC-CO2) were combined and modified to decellularize bovine cancellous bone (CB). After decellularization, almost 100% of ɑ-Gal epitopes were removed, the combination of collagen, calcium and phosphate was reserved. The direct and indirect contact with macrophages was used to evaluate the cytotoxicity and immunological response of the materials. Mesenchymal stem cells (MSCs) were used in the in vitro cells’ proliferation assay. The decellularized CB was proved has no cytotoxicity (grade 1) and no immunological response (NO, IL-2, IL-6 and TNF-α secretion inhibited), and could support MSCs proliferated continuedly. These results were similar to that of commercial decellularized human bone. This study suggests the potential of using this kind of combine decellularization process to fabricate heterogeneous ECM scaffolds for clinical application.
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Rashidi, Farina, Mahdi Mohammadzadeh, Arash Abdolmaleki, Asadollah Asadi und Mehrdad Sheikhlou. „Acellular carotid scaffold and evaluation the biological and biomechanical properties for tissue engineering“. Journal of Cardiovascular and Thoracic Research 16, Nr. 1 (13.03.2024): 28–37. http://dx.doi.org/10.34172/jcvtr.32899.

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Introduction: The issues associated with the limitation of appropriate autologous vessels for vascular reconstruction via bypass surgery highlight the need for new alternative strategies based on tissue engineering. The present study aimed to prepare decellularized scaffolds from ovine carotid using chemical decellularization method. Methods: Ovine carotid were decellularized with Triton X-100 and tri-n-butyl phosphate (TnBP) at 37 °C. Histological analysis, biochemical tests, biomechanical assay and biocompatibility assay were used to investigate the efficacy of decellularization. Results: Decellularization method could successfully decellularize ovine carotid without leaving any cell remnants. Scaffolds showed minimal destruction of the three-dimensional structure and extracellular matrix, as well as adequate mechanical resistance and biocompatibility for cell growth and proliferation. Conclusion: Prepared acellular scaffold exhibited the necessary characteristics for clinical applications.
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Brennan, Jordan, Michael L. Lu und Yunqing Kang. „A New Model of Esophageal Cancers by Using a Detergent-Free Decellularized Matrix in a Perfusion Bioreactor“. Bioengineering 10, Nr. 1 (11.01.2023): 96. http://dx.doi.org/10.3390/bioengineering10010096.

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The lack of physiologically relevant human esophageal cancer models has as a result that many esophageal cancer studies are encountering major bottleneck challenges in achieving breakthrough progress. To address the issue, here we engineered a 3D esophageal tumor tissue model using a biomimetic decellularized esophageal matrix in a customized bioreactor. To obtain a biomimetic esophageal matrix, we developed a detergent-free, rapid decellularization method to decellularize porcine esophagus. We characterized the decellularized esophageal matrix (DEM) and utilized the DEM for the growth of esophageal cancer cell KYSE30 in well plates and the bioreactor. We then analyzed the expression of cancer-related markers of KYSE30 cells and compared them with formalin-fixed, paraffin-embedded (FFPE) esophageal squamous cell carcinoma (ESCC) tissue biospecimens. Our results show that the detergent-free decellularization method preserved the esophageal matrix components and effectively removed cell nucleus. KYSE30 cancer cells proliferated well on and inside the DEM. KYSE30 cells cultured on the DEM in the dynamic bioreactor show different cancer marker expressions than those in the static well plate, and also share some similarities to the FFPE-ESCC biospecimens. These findings built a foundation with potential for further study of esophageal cancer behavior in a biomimetic microenvironment using this new esophageal cancer model.
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Bobrova, M. M., L. A. Safonova, O. I. Agapova, A. E. Efimov und I. I. Agapov. „The analysis of the proliferative activity of cells on microparticles obtained from decellularized liver and kidney tissue“. Russian Journal of Transplantology and Artificial Organs 20, Nr. 4 (31.01.2019): 69–75. http://dx.doi.org/10.15825/1995-1191-2018-4-69-75.

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Aim.To develop the protocols for liver and kidney tissue decellularization, and to develop an analysis of the proliferative activity of human Hep-G2hepatocarcinoma cells on various carriers.Materials and methods.Decellularization of the liver and kidneys was performed by perfusion of detergent solutions with gradually increasing concentrations of Triton X-100 (1, 2 and 3%). A histological analysis of the obtained samples was performed, and the method of optical and scanning electron microscopy was used to study the obtained samples. The proliferative activity of human Hep-G2hepatocarcinoma cells was studied on the obtained samples of decellularized liver and kidney tissue.Results.Decellularization of the organ does not lead to changes in the specific structure of the tissue matrix. Microparticles with an average size of 200 μm were made from their decellularized matrix of liver and kidney tissues. The level of proliferative activity of human Hep-G2hepatocarcinoma cells cultured on microparticles from a decellularized liver was significantly higher than on microparticles from a decellularized kidney.Conclusion.The decellularized matrix retains the native three-dimensional structure of the tissue. The level of cell proliferative activity is significantly higher on microparticles from the decellularized liver, which confirms the preservation of the specificity of the extracellular matrix of the tissue after the process of decellularization.
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Dissertationen zum Thema "Decellularized matrix"

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Shah, Mickey. „Cardiac Repair Using A Decellularized Xenogeneic Extracellular Matrix“. University of Akron / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=akron1542631193281779.

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Marengo, Kaitlyn A. „The Incorporation of Decellularized Cardiac ECM into Fibrin Microthreads“. Digital WPI, 2017. https://digitalcommons.wpi.edu/etd-theses/843.

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Stem cell therapies have shown promising capabilities in regaining the functionality of scar tissue following a myocardial infarction. Biological sutures composed of fibrin have been shown to more effectively deliver human mesenchymal stem cells (hMSCs) to the heart when compared to traditional cell delivery mechanisms. While the biological sutures do show promise, improvements can be made. To enhance the fibrin sutures, we propose to incorporate native cardiac extracellular matrix (ECM) into the fibrin microthreads to produce a more in vivo-like environment. This project investigated the effects that ECM incorporation has on fibrin microthread structure, mechanics, stem cell seeding, and pro-angiogenic potential. Single microthreads composed of fibrin or fibrin and ECM were subjected to uniaxial tensile testing. It was found that the microthreads consisting of both fibrin and ECM had significantly high elastic moduli than fibrin only microthreads. Cell seeding potential was evaluated by performing a 24-hour hMSC seeding experiment using sutures of the varying microthread types. A CyQuant cell proliferation assay was used to determine the number of cells seeded onto each suture type. The results determined that there was no statistical difference between the numbers of cells seeded on the types of sutures. To examine the pro-angiogenic potential the microthreads had, a 24-hour endothelial progenitor outgrowth cell (EPOC) outgrowth assay was used. Fibrin and 15% ECM-fibrin microthreads were placed within the scratch of an EPOC culture and evaluated every 6 hours for 24 hours. We found that the 15% ECM microthreads had significantly increased the EPOC outgrowth, approximately 16% more distance travelled than fibrin microthreads and 18% more than no microthreads. Our combined results suggest that ECM does not affect hMSC attachment to biological sutures but does increase the pro-angiogenic potential of the microthreads due to their increase in guiding EPOC outgrowth.
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Young, Bethany M. „Novel Small Airway Model Using Electrospun Decellularized Lung Extracellular Matrix“. VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4273.

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Chronic respiratory diseases affects many people worldwide with little known about the mechanisms diving the pathology, making it difficult to find a cure. Improving the understanding of smooth muscle and extracellular matrix (ECM) interaction is key to developing a remedy to this leading cause of death. With currently no relevant or controllable in vivo or in vitro model to investigate diseased and normal interactions of small airway components, the development of a physiologically relevant in vitro model with comparable cell attachment, signaling, and organization is necessary to develop new treatments for airway disease. The goal of this study is to create a mechanically, biologically and structurally relevant in vitro model of small airway smooth muscle tissue. Synthetic Poly-L-Lactic Acid (PLLA) and decellularized pig lung ECM (DPLECM) were electrospun to form nanofibrous mats that can closely mimic natural bronchial tissue. The addition of DPLECM significantly changed the PLLA scaffold mechanically, biologically, and physically to bring it closer to the characteristics of the human lung. DPLECM scaffolds exhibited a significant decrease in the elastic modulus compared with PLLA alone. Histological staining and SDS-PAGE showed that after scaffold fabrication, essential proteins or protein fragments in natural ECM are still present after processing. Human bronchial smooth muscle cells (HBSMCs) seeded onto PLECM scaffolds formed multiple layers of cells compared to scaffolds composed solely of PLLA. Phenotype of smooth muscle is better maintained when DPLECM is incorporated into the scaffold shown by enhanced contractile protein expression and increased collagen production for normal smooth muscle remodeling of the scaffold. In summary, this research demonstrates that a PLLA/DPLECM composite electrospun mat is a promising tool to produce an in vitro model with the potential to uncover unknown characteristics of bronchiole smooth muscle behavior in diseased or normal states.
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Li, Zhaoying. „Adaptive fabrication of biofunctional decellularized extracellular matrix niche towards complex engineered tissues“. Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/270349.

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Recreating organ-specific microenvironments of the extracellular matrix (ECM) in vitro has been an ongoing challenge in biofabrication. In this study, I present a biofunctional ECM-mimicking protein scaffold with tunable biochemical, mechanical and topographical properties. This scaffold, formed by microfibres, displays three favorable characteristics as a cell culture platform: high-loading of key ECM proteins, single-layered mesh membrane with controllable mesh size, and flexibility for supporting a range of cell culture configurations. Decellularized extracellular matrix (dECM) powder was used to fabricate this protein scaffold, as a close replicate of the chemical composition of physiological ECM. The highest dECM concentration in the solidified protein scaffold was 50 wt%, with gelatin consisting the rest. In practice, a high density of dECM-laden nano- to microfibres was directly patterned on a variety of substrates to form a single layer of mesh membrane, using the low-voltage electrospinning patterning (LEP) method. The smallest fibre diameter was measured at 450 nm, the smallest mesh size of the membrane was below 1 μm, and the thickness of the membrane was estimated to be less than 2 μm. This fabrication method demonstrated a good preservation of the key ECM proteins and growth factors, including collagen IV, laminin, fibronectin, VEGF and b-FGF. The integrated fibrous mesh exhibited robust mechanical properties, with tunable fibril Young’s modulus for over two orders of magnitude in the physiological range (depending on the dECM concentration). Combining this mesh membrane with 3D printing, a cell culture device was constructed. Co-culture of human glomerulus endothelial cells and podocytes was performed on this device, to simulate the blood-to-urine interface in vitro. Good cell attachment and viability were demonstrated, and specific cell differentiation and fibronectin secretion were observed. This dECM-laden protein scaffold sees the potential to be incorporated into a glomerulus-on-chip model, to further improve the physiological relevance of in vitro pathological models.
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D'Angelo, Edoardo. „Decellularized colorectal cancer matrix as bioactive microenvironment for in vitro 3D cancer research“. Doctoral thesis, Università degli studi di Padova, 2018. http://hdl.handle.net/11577/3426811.

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Three-dimensional (3D) cancer models are overlooking the scientific landscape with the primary goal of bridging the gaps between two-dimensional (2D) cell cultures, animal models and clinical research. In this thesis, we describe an innovative tissue engineering approach applied to colorectal cancer (CRC) starting from decellularized human biopsies in order to generate an organotypic 3D bioactive model. This in vitro 3D system recapitulates the ultrastructural environment of native tissue as demonstrated by histology, immunohistochemistry, immunofluorescence and scanning electron microscopy analyses. Mass spectrometry of proteome and secretome confirmed a different stromal composition between decellularized healthy mucosa and CRC in terms of structural proteins (COL1A1, COL1A2, and COL3A1) and secreted proteins such as DEFA3. Importantly, we proved that our 3D acellular matrices retained their biological properties: using CAM assay, we observed a decreased angiogenic potential in decellularized CRC compared with healthy colon mucosa, caused by direct effect of DEFA3. In addition, we demonstrated that following a 5 days of recellularization with HT-29 cell line, the 3D tumor matrices induced an over-expression of IL-8, a DEFA3-mediated pathway and a mandatory chemokine in cancer growth and proliferation, compared with recellularized healthy mucosa and 2D conventional culture model. Given the biological activity maintained by the scaffolds after decellularization, we believe this approach is a powerful tool for future pre-clinical research and screenings.
I modelli tumorali tridimensionali (3D) si stanno affacciando sul panorama scientifico con l’obiettivo primario di superare le limitazioni di colture cellulari convenzionali (2D) e modelli animali negli approcci di ricerca clinica. In questa tesi di dottorato, si descrive un innovativo approccio di ingegneria tissutale applicata alla ricerca oncologica mediante il quale, partendo da una biopsia tissutale decellularizzata, si genera un modello organo-tipico 3D bioattivo. Questo modello 3D, ricapitola, in vitro, l’ambiente ultra-strutturale del tessuto nativo come dimostrato da indagini istologiche, immunoistochimiche, di immunofluorescenza e di microscopia elettronica a scansione. L’analisi del proteoma e del secretoma mediante spettrometria di massa ha confermato una differente composizione stromale tra la mucosa colica sana decellularizzata e quella della controparte tumorale (CRC) in termini di proteine strutturali (Collagene 1A1, Collagene 1A2, Collagene 3A1) e di proteine secrete, come la Defensina alfa 3. Abbiamo dimostrato che le nostre matrici 3D mantengono le loro proprietà biologiche dopo il processo di decellularizzazione: mediante la CAM, abbiamo osservato un decremento del potenziale angiogenico della matrice decellularizzata di CRC comparata con la mucosa colica sana, causata da un effetto diretto della Defensina alfa 3. Inoltre, abbiamo dimostrato che dopo 5 giorni di ricellularizzazione con cellule HT-29 (linea stabilizzata di cancro del colon), le matrici tumorali 3D (comparate con le rispettive mucose coliche sane ed il metodo di coltura 2D) hanno indotto una sovra-espressione di IL-8, una chemochina a valle del pathway della Defensina alfa 3, che gioca un ruolo molto importante nella crescita e proliferazione tumorale. In conclusione, avendo dimostrato la capacità dei delle nostre matrici acellulari 3D di mucosa colica sana e CRC di mimare gli stimoli ultra-strutturali e biologici dei rispettivi tessuti nativi, crediamo che questo approccio possa essere un efficace strumento per migliorare il livello delle ricerche precliniche e nei test di screening di farmaci.
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KC, Pawan. „Development of a Cardiac Patch with Decellularized Myocardial Tissue and Stem Cells“. University of Akron / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=akron1555413717363006.

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Hansen, Ryan. „Functional and Structural Analysis of Decellularized Liver Tissue Matrix, with Potential Applications in Cancer Tissue Engineering“. Case Western Reserve University School of Graduate Studies / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1498650461817088.

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Miyauchi, Yuya. „A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells“. Kyoto University, 2018. http://hdl.handle.net/2433/232113.

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Trignol, Aurélie. „The extracellular matrix as a biomaterial to optimize skeletal muscle regeneration“. Thesis, Lyon, 2019. http://www.theses.fr/2019LYSE1029.

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Le muscle strié squelettique possède de grandes capacités de régénération grâce à ses cellules souches, les cellules satellites. Après une lésion, le processus de régénération musculaire qui se met en place est finement régulé dans le temps et l’espace par le microenvironnement, constitué de cellules avoisinantes mais également par des éléments de la matrice extracellulaire (MEC). Cette dernière se compose de molécules structurales comme les collagènes et de composants possédant un rôle trophique comme les glycosaminoglycanes (GAGs). La MEC musculaire est peu étudiée à cause d’une organisation tridimensionnelle complexe rendant son exploration difficile. Lors d’une lésion avec perte de substance musculaire, la régénération est altérée, associée à une fibrose et une inflammation chronique. Ce type de lésion est fréquemment rencontré en traumatologie mais survient également chez le blessé de guerre. Malgré un traitement optimal, une invalidité fonctionnelle persiste chez ces patients. L’utilisation d’un biomatériau décellularisé, constitué de MEC pourrait fournir ce support physique et trophique faisant défaut dans ce type de lésion. Dans ce travail, nous avons entrepris l'établissement d'une MEC d’origine musculaire et nous avons établi un protocole de décellularisation permettant d’obtenir un biomatériau conservant l’architecture spécifique de la MEC musculaire avec une élimination de la majorité des antigènes cellulaires afin d'éviter une réponse immunitaire délétère après implantation. Néanmoins, le protocole retenu ne permet de conserver certaines molécules trophiques d’intérêt comme les GAGs. Les « ReGeneRaTing Agent®» (RGTA®) sont des mimétiques fonctionnels de ces GAGs, utilisés en clinique pour améliorer la cicatrisation cutanée et cornéenne. Ces mimétiques conservent une capacité de liaison aux facteurs de croissance avec une résistance aux dégradations enzymatiques. Nous avons évalué l’utilisation de ces molécules au cours de la réparation musculaire, dans un modèle in vivo chez le rongeur. Nous avons réalisé une analyse histologique précoce (8e jour de régénération) mettant en évidence une augmentation du nombre de noyaux par myofibre en faveur d’une augmentation de la fusion, validée également in vitro sur des progéniteurs musculaires. Nous avons également observé une augmentation du nombre de vaisseaux, suggérant une amélioration de l’angiogenèse. Le nombre de gouttelettes lipidiques, marqueur d’une mauvaise régénération, était en diminution. L’exploration histologique plus tardive (28e jour de régénération) n’a retrouvé que l’augmentation du nombre de vaisseaux en faveur d’un effet durable sur l’angiogenèse. Ces RGTA® peuvent être couplés aux biomatériaux et sont particulièrement résistants dans un environnement inflammatoire pouvant être rencontré dans les lésions avec perte de substance musculaire. Des chimiokines et des facteurs de croissance pourront également être ajoutés au biomatériau matriciel afin de favoriser la migration des différents progéniteurs nécessaires à une néoformation musculaire. L’efficacité thérapeutique de ces biomatériaux optimisés nécessitera d’être évaluée dans un modèle in vivo de perte de substance
Skeletal muscle exhibits high capacity for regeneration after an injury that relies on resident stem cells. Muscle regeneration is tightly regulated by both the immune response and other resident cells, as well as by cues from the local extracellular matrix (ECM), contributing to a coordinated repair process. Muscle ECM is a network of structural macromolecules with a large majority of collagens and trophic molecules such as glycosaminoglycans (GAGs). In the skeletal muscle tissue, ECM was overlooked due to its complex organization making investigations difficult. Muscle regenerative ability can be overtaken in large muscle wasting, such as in volumetric muscle loss (VML), leading to fibrosis formation and chronic inflammation. This type of injury predominantly occurs in traumatology and in war-wounded patients, with functional disability despite an optimal treatment. The use of biomaterials could provide the biochemical and physical cues that are missing in this pathologic repair. In this work we have focused on obtaining a biomaterial composed of skeletal muscle ECM. We have tested several decellularization protocols both to preserve the three-dimensional architecture of the muscle ECM and to completely remove cell components in order to avoid a deleterious immune response after implantation. However, the protocol did not allow the preservation of trophic molecules such as GAGs, in the scaffold.“ReGenerating Agents” (RGTA®) are functionally analogous of GAGs with a crucial property to resist enzymatic degradation. They function to restore a proper microenvironment for tissue healing with already a clinical application in skin and corneal repair. We have explored the effects of RGTA® in muscle regeneration using an in vivo model in mouse. At early time of regeneration (day 8), we performed histologic analysis. We showed that regenerating myofibers contained more nuclei in the treated animals, in favor of an increase of progenitor fusion, which has been validated in vitro in myogenic cultures. The number of capillaries was higher in favor of a better angiogenesis. Lipid droplets, a marker of impaired regeneration, were reduced by RGTA® administration. At later time of regeneration (day 28), capillary number was still improved in favor of a durable effect of RGTA® on angiogenesis. RGTA® could be incorporated into biomaterials and are particularly resistant in an inflammatory environment, such as that occurring after a VML injury. Chemokines and growth factors could also be added in ECM-based scaffolds to promote the migration of progenitors that are essential for myofiber neoformation. Therapeutic efficacy of these optimized biomaterials will require to be evaluated in an in vivo model of VML
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Pouliot, Robert A. „DEVELOPMENT AND CHARACTERIZATION OF LUNG DERIVED EXTRACELLULAR MATRIX HYDROGELS“. VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4465.

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Chronic obstructive pulmonary disease (COPD) including emphysema is a devastating condition, increasing in prevalence in the US and worldwide. There remains no cure for COPD, rather only symptomatic treatments. Due to unique challenges of the lung, translation of therapies for acute lung injury to target chronic lung diseases like COPD has not been successful. We have been investigating lung derived extracellular matrix (ECM) hydrogels as a novel approach for delivery of cellular therapies to the pulmonary system. During the course of this work we have developed and characterized a lug derived ECM hydrogel that exhibits “injectability,” allowing cells or dugs to be delivered in a liquid and encapsulated at body temperature. The hydrogel self assembles in <5 minutes and achieves mechanical stiffness similar to other soft tissue ECM hydrogels. The hydrogel can support 3D cell growth and encapsulated cell viability. Encapsulated hMSCs can also still be activated by simulated inflammatory environments. Naïve mouse macrophages exposed to the fully formed gel were not significantly induced to express markers for pro or anti-inflammatory polarized phenotypes, but increased expression for several secreted inflammatory mediators was observed. We also investigated a novel approach for preparing and solubilizing the isolated ECM proteins, using digestion time as a variable for controlling hydrogel density (interconnectivity), mechanical stiffness, component protein size distribution, and cell behavior on fully formed gels. The potential future impact for the presented research includes optimization for future animal studies, expansion to additional applications, and the development of new derivative materials.
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Bücher zum Thema "Decellularized matrix"

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Yamaoka, Tetsuji, und Takashi Hoshiba, Hrsg. Decellularized Extracellular Matrix. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998.

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Yamaoka, Tetsuji, und Takashi Hoshiba. Decellularized Extracellular Matrix: Characterization, Fabrication and Applications. Royal Society of Chemistry, The, 2019.

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Buchteile zum Thema "Decellularized matrix"

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Hoshiba, Takashi, und Tetsuji Yamaoka. „CHAPTER 1. Extracellular Matrix Scaffolds for Tissue Engineering and Biological Research“. In Decellularized Extracellular Matrix, 1–14. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00001.

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Yamaoka, Tetsuji. „CHAPTER 2. Preparation Methods for Tissue/Organ-derived dECMs – Effects on Cell Removal and ECM Changes“. In Decellularized Extracellular Matrix, 15–28. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00015.

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Hoshiba, T., N. Kawazoe und G. Chen. „CHAPTER 3. Preparation of Cultured Cell-derived Decellularized Matrix (dECM) – Factors Influencing dECM Formation and Its Ability“. In Decellularized Extracellular Matrix, 29–50. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00029.

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Mochitate, Katsumi, Reiko Nagano und Yukiko Toya-Nakajima. „CHAPTER 4. Bared Basement Membrane Substrata: Design, Cellular Assembly, Decellularization and Application to Tissue Regeneration and Stem Cell Differentiation“. In Decellularized Extracellular Matrix, 51–76. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00051.

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Morimoto, Naoki, Atsushi Mahara und Tetsuji Yamaoka. „CHAPTER 5. A Novel Treatment for Giant Congenital Melanocytic Nevi Combining Inactivated Nevus Tissue by Pressurization and Cultured Epidermal Autograft“. In Decellularized Extracellular Matrix, 77–94. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00077.

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Scanameo, Alexandra, und Nicholas P. Ziats. „CHAPTER 6. Immune Responses to Decellularized Matrices“. In Decellularized Extracellular Matrix, 95–115. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00095.

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Duran, Pamela, Marianna Alperin und Karen L. Christman. „CHAPTER 7. Decellularized Extracellular Matrix Hydrogels: Fabrication, Properties, Characterization, and Current Applications“. In Decellularized Extracellular Matrix, 116–38. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00116.

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Das, Sanskrita, Anthony Safaa Mukhtar, Jinah Jang und Jin-Hyung Shim. „CHAPTER 8. Decellularized Extracellular Matrix as Bioink for 3D-Bioprinting“. In Decellularized Extracellular Matrix, 139–62. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00139.

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Hwang, Mintai P., und Kwideok Park. „CHAPTER 9. Mechanical Property Tunable dECM and Their Regenerative Applications“. In Decellularized Extracellular Matrix, 163–78. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00163.

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Hodde, J. P. „CHAPTER 10. Use of Small Intestinal Submucosa dECM in Tissue Engineering and Regenerative Medicine“. In Decellularized Extracellular Matrix, 179–98. Cambridge: Royal Society of Chemistry, 2019. http://dx.doi.org/10.1039/9781788015998-00179.

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Konferenzberichte zum Thema "Decellularized matrix"

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Shchotkina, Nataliia V., Anatoliy A. Sokol, Glib I. Yemets, Oleksandr Yu Galkin, Liudmyla V. Dolinchuk, Iryna M. Skorokhod, Olena V. Shepeleva, Nadiia M. Rudenko und Iliia M. Yemets. „Microarchitectonic of Decellularized Bovine Pericardium Matrix“. In The 7th World Congress on New Technologies. Avestia Publishing, 2021. http://dx.doi.org/10.11159/icbb21.167.

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Tas, S., D. A. Bölükbas, H. N. Alsafadi, I. A. Da Silva, M. M. De Santis, E. Rehnberg, I. Tamargo, S. Mohlin, S. Lindstedt und D. E. Wagner. „Decellularized extracellular matrix hydrogels for human airway organoid culture“. In ERS Lung Science Conference 2021 abstracts. European Respiratory Society, 2021. http://dx.doi.org/10.1183/23120541.lsc-2021.101.

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Kuhlin, B., J. Kern, D. Gvaramia, N. Rotter, H. Tritschler, Y. Jakob, L. Körber, R. Breiter und RE Brenner. „Detection of matrix metalloproteinases after seeding a decellularized extracellular matrix with chondrogenic progenitor cells“. In Abstract- und Posterband – 90. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn – Digitalisierung in der HNO-Heilkunde. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1686890.

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Gvaramia, D., J. Kern, Y. Jakob, L. Huber und N. Rotter. „The Response of Primary Human Macrophages to Decellularized Cartilage Extracellular Matrix“. In Abstract- und Posterband – 91. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn – Welche Qualität macht den Unterschied. © Georg Thieme Verlag KG, 2020. http://dx.doi.org/10.1055/s-0040-1711446.

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Price, AP, TR Metz und A. Panoskaltsis-Mortari. „Decellularized Lung as a 3-D Matrix for Bioengineering the Lung.“ In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a5382.

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Gvaramia, D., J. Kern, Y. Jakob, L. Huber, J. Kzhyshkowska und N. Rotter. „The Response of Primary Human Macrophages to Decellularized Cartilage Extracellular Matrix“. In 100 JAHRE DGHNO-KHC: WO KOMMEN WIR HER? WO STEHEN WIR? WO GEHEN WIR HIN? Georg Thieme Verlag KG, 2021. http://dx.doi.org/10.1055/s-0041-1728945.

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Zhao, Shijia, Linxia Gu, James M. Hammel und Haili Lang. „Mechanical Characterization of the Decellularized Porcine Small Intestinal Submucosa Extracellular Matrix“. In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-65640.

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In this work, the decellularized porcine small intestinal submucosa extracellular matrix (SIS-ECM), obtained from the commercial product under the trade name of CorMatrix, were tested in uniaxial tension. Preconditioning under cyclic loading of 2 N was conducted to stabilize the mechanical response of the tissue. The influence of rehydration time on the mechanical properties of the tissue was evaluated. Results suggested that the stiffness of SIS-ECM decreased with longer rehydration time. Considering the application of CorMatrix in pericardial closure, the native pericardium samples were also tested. The comparison indicated that the native pericardium is softer than rehydrated CorMatrix. This work can facilitate the surgeons to better choose the appropriate rehydration time when conducting the extracardiac implantations, such as pericardial reconstruction, pericardial closure, etc.
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Sinem, Tas, Deniz A. Bölükbas, Hani N. Alsafadi, Iran An Da Silva, Martina M. De Santis, Emil Rehnberg, Isabel Tamargo, Sophie Mohlin, Sandra Lindstedt und Darcy E. Wagner. „LSC - 2021 - Decellularized extracellular matrix hydrogels for human airway organoid culture“. In ERS International Congress 2021 abstracts. European Respiratory Society, 2021. http://dx.doi.org/10.1183/13993003.congress-2021.oa1614.

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Wirtzfeld, L. A., E. S. L. Berndl und M. C. Kolios. „Ultrasonic characterization of extra-cellular matrix in decellularized murine kidney and liver“. In 2015 IEEE International Ultrasonics Symposium (IUS). IEEE, 2015. http://dx.doi.org/10.1109/ultsym.2015.0170.

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Godin, Lindsay M., Andrew P. Price und Angela Panoskaltsis-Mortari. „Characterization Of Decellularized Lung Matrix After FITC-Induced Lung Injury In Mice“. In American Thoracic Society 2012 International Conference, May 18-23, 2012 • San Francisco, California. American Thoracic Society, 2012. http://dx.doi.org/10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a1030.

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