Auswahl der wissenschaftlichen Literatur zum Thema „D. B. Dutta“

The p21(WAF1/CIP1/sdi1) gene product (WAF1) inhibits DNA replication in vitro (J. Chen, P. Jackson, M. Kirschner, and A. Dutta, Nature 374:386-388, 1995; S. Waga, G. Hannon, D. Beach, and B. Stillman, Nature 369:574-578, 1994), but in vivo studies on the antiproliferative activity of WAF1 have not resolved G1-phase arrest from potential inhibition of S-phase progression. Here, we demonstrate that elevated WAF1 expression can retard replicative DNA synthesis in vivo. The WAF1-mediated inhibitory effect could be antagonized by cyclin A, cyclin E, or the simian virus 40 small-t antigen with no decrease in the levels of WAF1 protein in transfected cells. Proliferating-cell nuclear antigen (PCNA) overexpression was neither necessary nor sufficient to antagonize WAF1 action. Expression of the N-terminal domain of WAF1, responsible for cyclin-dependent kinase (CDK) interaction, had the same effect as full-length WAF1, while the PCNA binding C terminus exhibited modest activity. We conclude that S-phase progression in mammalian cells is dependent on continuing cyclin and CDK activity and that WAF1 affects S phase primarily through cyclin- and CDK-dependent pathways.

ABSTRACT Rising number of infertile males in India provokes the need of evidence-based comprehensive formula addressing unattended causes of male infertility. There is an innovative product*, a combikit available in Indian market, containing 30 tablets of antioxidant, multivitamin, amino acid and mineral tablets, with documented evidence on ingredients in treatment of male infertility, and 25 tablets of clomiphene citrate which is a timetested drug in treatment of male infertility (in particular oligospermia and/or asthenospermia). In order to assess efficacy of the product in Indian infertile male patients, we undertook phase IV multicentric clinical study, at 18 centers across India in 100 patients. Very high couple pregnancy rate of 53% was reported. As first line treatment of male infertility (in particular oligospermia and/or asthenospermia), the use of this innovative product therefore holds a strong promise. How to cite this article Amin J, Mishra SK, Dutta AK, Kunwar K, Shinde VG, Chauhan A, Sud S, Maurya R, Nepal N, Sarode P, Biswas KP, Mazumder GP, Chitrabanshi M, Shibe S, Shukla R, Trivedi N, Dayal M, Sortey D, Shukla S, Gawade B. Indian Clinical Experience on Innovative Product in Treatment of Male Infertility. J South Asian Feder Obst Gynae 2013;5(2):76-79.

Managing liquid water distribution in PEFCs is critical to desirable high power density operation and cell durability [1–3]. Degradation modes such as carbon corrosion in the cathode catalyst layer (CCL) are believed to depend on local humidification [2,4], while the distribution of liquid water may also be influenced by performance drop associated with such degradation [3]. Although some studies have shown the influence of carbon corrosion on liquid water distribution [2,3], the possible reverse effect of liquid water distribution on the CCL degradation requires further investigation. The objective of the present work is to establish a deeper understanding of the cause-and-effect interactions between CCL degradation and liquid water distribution. This is achieved experimentally by carrying out voltage-cycling accelerated stress tests (ASTs) on fuel cells which differ only in the constituent gas diffusion layers (GDLs); namely, SGL 22 BB and a proprietary Avcarb. Three-dimensional X-ray microscopy is used to observe both degradation effects and liquid water distribution at different stages of the ASTs. A relatively rapid operando two-dimensional visualization technique [5] was also used to investigate the differences in liquid water distribution between the two GDLs. The fuel cells imaged are analyzed and exhibit different liquid water distributions, whereby the Avcarb exhibits a higher liquid water condensation close to the CCL, compared to the SGL. Supported by model results, this difference in liquid water distribution is shown to be attributable to the different transport properties of the GDLs. The degradation results, such as the CCL thickness (Fig. 1) show that the Avcarb cell experiences a faster CCL degradation than the SGL cell. Furthermore, the Avcarb liquid water distribution is seen to change with increasing AST cycles in a manner indicating higher vapour phase removal. These results provide insights for GDL design consideration, showing that GDL transport properties may influence liquid water distribution at the electrode with important implications for CCL durability. Acknowledgement Funding for this research was provided by the Natural Sciences and Engineering Research Council of Canada, Ballard Power Systems, Canada Foundation for Innovation, British Columbia Knowledge Development Fund, and Canada Research Chairs. References [1] F. Nandjou, J.-P. Poirot-Crouvezier, M. Chandesris, J.-F. Blachot, C. Bonnaud, and Y. Bultel, “Impact of heat and water management on proton exchange membrane fuel cells degradation in automotive application,” Journal of Power Sources, vol. 326, pp. 182–192, 2016. [2] J. D. Fairweather, D. Spernjak, A. Z. Weber, D. Harvey, S. Wessel, D. S. Hussey, D. L. Jacobson, K. Artyushkova, R. Mukundan, and R. L. Borup, “Effects of cathode corrosion on through-plane water transport in proton exchange membrane fuel cells,” Journal of The Electrochemical Society, vol. 160, no. 9, p. F980, 2013. [3] R. T. White, S. H. Eberhardt, Y. Singh, T. Haddow, M. Dutta, F. P. Orfino, and E. Kjeang, “Four-dimensional joint visualization of electrode degradation and liquid water distribution inside operating polymer electrolyte fuel cells,” Scientific reports, vol. 9, no. 1, p. 1843, 2019. [4] T. Mittermeier, A. Weiß, F. Hasché, and H. A. Gasteiger, “Pem fuel cell start-up/shut-down losses vs relative humidity: the impact of water in the electrode layer on carbon corrosion,” Journal of The Electrochemical Society, vol. 165, no. 16, p. F1349, 2018. [5] F. Aroge, B. Parimalam, J. MacDonald, F. Orfino, M. Dutta, and E. Kjeang, “Analysing operando 2d x-ray transmission images for liquid water distribution in polymer electrolyte fuel cells,” Journal of Power Sources, vol. 564, p. 232820, 2023. Figure 1

This paper presents sensor structure dedicated for determination the key properties of blood – concentration level and oxidation level. The sensor structure is based integrated optics circuit with diamond-based planar waveguide including prism and grating coupler. The paper is focused on numerical analysis of selected properties of sensor structure for optimization sensing parameters. Full Text: PDF ReferencesD.A. Gell, Blood Cells, "Structure and function of haemoglobins", Molecules and Diseases 70 (2018). CrossRef I. Singh, A. Weston, A. Kundur, G. Dobie, Haematology Case Studies with Blood Cell Morphology and Pathophysiology (Elsevier: Amsterdam, The Netherlands, 2017) DirectLink P. D. Sturkie, P. Griminger, Blood: Physical Characteristics, Formed Elements, Hemoglobin, and Coagulation Avian Physiology (Springer-Verlag New York Inc. 1976). CrossRef A. Dutta, B. Deka, P.P. Partha, Planar Waveguide Optical Sensors: From Theory to Applications (Springer: Berlin, Germany, 2016). CrossRef S. Kang, K. Sasaki, H. Minamitani, "Determining the absorption coefficient of hemoglobin derivatives with integrated optic waveguide sensor", IEEE Engineering in Medicine and Biology Society (1992). CrossRef P. Struk, "Design of an Integrated Optics Sensor Structure Based on Diamond Waveguide for Hemoglobin Property Detection", Materials 12(1) 175 (2019). CrossRef W. Lukosz, K. Tiefenthaler, "Sensitivity of integrated optical grating and prism couplers as (bio)chemical sensors", Sensors and Actuators 15 (1988). CrossRef P.V. Lambeck, "Integrated optical sensors for the chemical domain", Measurement Science and Technology - IOPscience 17 (2006), CrossRef W. Lukosz, "Integrated optical chemical and direct biochemical sensors", Sensors and Actuators B: Chemical 29 (1995). CrossRef P. Struk, T. Pustelny, K. Gołaszewska, E. Kamińska, M. Borysiewicz, M. Ekielski, And A. Piotrowska, "Photonic structures with grating couplers based on ZnO", Opto−Electronics Review 19(4) (2011). CrossRef J. Haas, E. V. Catalán, P. Piron, F. Nikolajeff, L. Österlund, M. Karlsson, B. Mizaikoff, "Polycrystalline Diamond Thin-Film Waveguides for Mid-Infrared Evanescent Field Sensors", ACS Omega 3 (2018). CrossRef P. Struk, "Design of an integrated optics sensor structure for hemoglobin property detection", Proceedings of SPIE 11204 (2019). CrossRef P. Struk, T. Pustelny, K. Gołaszewska, E. Kamińska, M. Borysiewicz, M. Ekielski, A. Piotrowska, "Hybrid photonics structures with grating and prism couplers based on ZnO waveguides", Opto-Electronics Review 21 (2013). CrossRef V. Prajzler, M. Varga, P. Nekvindova, Z.; Remes, A. Kromka, "Design and investigation of properties of nanocrystalline diamond optical planar waveguides", Optics Express 21 (2013), CrossRef E.N. Lazareva, V.V. Tuchin, "Measurement of refractive index of hemoglobin in the visible/NIR spectral range", Journal of Biomedical Optics 23 (2018). CrossRef Optiwave Systems Inc. "OptiFDTD Technical Background and Tutorials - Finite" (2013). DirectLink K. Yee, Antennas and Propagation, "Numerical solution of initial boundary value problems involving maxwell's equations in isotropic media", IEEE Transactions 14,3 (1966). CrossRef

We explore various aspects of electrochemistry and photoelectrochemistry using in situ spectroscopy of electrode (metal) and photoelectrode (semiconductor) interfaces under electrochemical working conditions. Using sum frequency generation (SFG) spectroscopy, we measure the voltage dependence of the orientation of D2O molecules at a graphene electrode surface, which enables us to extract the free energy orienting potential of interfacial water.1 In particular, we measure the “free OD” feature in the spectra, which corresponds to the topmost water molecule that is rotated up out of the bulk water solution and is, therefore, not hydrogen bonded. Using transient absorption spectroscopy (TAS), we measure the lifetime of hot electrons photoexcited in plasmon resonant nanostructures.5 In a similar study, we use transient reflectance spectroscopy (TRS) to measure the photoexcited carrier dynamics in a GaP/TiO2 photoelectrode, as well as the electrostatic field dynamics at this semiconductor-liquid interface in situ under various electrochemical potentials.2 Here, the electrostatic fields at the surface of the semiconductor are measured via Franz−Keldysh oscillations (FKO). These spectra reveal that the nanoscale TiO2 protection layer enhances the built-in field and charge separation performance of GaP photoelectrodes. Using surface enhanced Raman scattering (SERS) spectroscopy, we monitor local electric fields via Stark-shifts of nitrile-functionalized silicon photoelectrodes.6 By monitoring Stark shifts in graphene-enhanced Raman spectroscopy (GERS), we measure local electric fields and local charge densities at monolayer graphene electrode surfaces.3 Lastly, we measure the stacking dependence of monolayer WSe2/MoSe2 heterostructures and observe resonant excitation of interlayer excitons for photocatalytic energy conversion.4 Montenegro, A., C. Dutta, M. Mammetkuliev, H.T. Shi, B.Y. Hou, D. Bhattacharyya, B.F. Zhao, S.B. Cronin and A.V. Benderskii, Asymmetric response of interfacial water to applied electric fields. Nature, 594, 62 (2021). Xu, Z.H., B.Y. Hou, F.Y. Zhao, Z. Cai, H.T. Shi, Y.W. Liu, C.L. Hill, D.G. Musaev, M. Mecklenburg, S.B. Cronin and T.Q. Lian, Nanoscale TiO2 Protection Layer Enhances the Built-In Field and Charge Separation Performance of GaP Photoelectrodes. Nano Letters, 21, 8017-8024 (2021). Shi, H.T., B.F. Zhao, J. Ma, M.J. Bronson, Z. Cai, J.H. Chen, Y. Wang, M. Cronin, L. Jensen and S.B. Cronin, Measuring Local Electric Fields and Local Charge Densities at Electrode Surfaces Using Graphene-Enhanced Raman Spectroscopy (GERS)-Based Stark-Shifts. ACS Applied Materials & Interfaces, 11, 36252-36258 (2019). Chen, J., C.S. Bailey, D. Cui, Y. Wang, B. Wang, H. Shi, Z. Cai, E. Pop, C. Zhou and S.B. Cronin, Stacking Independence and Resonant Interlayer Excitation of Monolayer WSe2/MoSe2 Heterostructures for Photocatalytic Energy Conversion. ACS Applied Nano Materials, DOI:10.1021/acsanm.9b01898 (2020). Yu Wang, Yi Wang, Indu Aravind, Zhi Cai, Lang Shen, Boxin Zhang, Bo Wang, Jihan Chen, Bofan Zhao, Haotian Shi, Jahan M. Dawlaty, and Stephen B. Cronin. In Situ Investigation of Ultrafast Dynamics of Hot Electron-Driven Photocatalysis in Plasmon-Resonant Grating Structures. Journal of the American Chemical Society. DOI: 10.1021/jacs.1c12069 (2022). Haotian Shi, Ryan T. Pekarek, Ran Chen, Boxin Zhang, Yu Wang, Indu Aravind, Zhi Cai, Lasse Jensen, Nathan R. Neale, and Stephen B. Cronin. Monitoring Local Electric Fields using Stark Shifts on Napthyl Nitrile-Functionalized Silicon Photoelectrodes. The Journal of Physical Chemistry C, 124, 17000-17005 (2020).

We report various aspects of electrochemistry and photoelectrochemistry using in situ spectroscopy of electrode (metal) and photoelectrode (semiconductor) interfaces in situ under electrochemical working conditions. These spectroscopies include sum frequency generation (SFG), transient reflectance/absorption spectroscopy (TAS/TRS), and surface enhanced Raman spectroscopy (SERS). Using surface enhanced Raman scattering (SERS) spectroscopy, we monitor local electric fields using Stark-shifts of nitrile-functionalized silicon photoelectrodes.6 Using Graphene-enhanced Raman spectroscopy (GERS)-based Stark-shifts, we measure local electric fields and local charge densities at monolayer graphene electrode surfaces.1 We also measured the stacking dependence and Resonant interlayer excitation of monolayer WSe2/MoSe2 heterostructures for photocatalytic energy conversion.2 Using sum frequency generation (SFG) spectroscopy, we measure the voltage dependence of the orientation of D2O molecules at a graphene electrode surface, which is related back to the “stiffness of the ensemble”.3 In particular, we measured the “free OD” feature in the spectra, which corresponds to the topmost water molecule that is rotated up out of the bulk water solution and is, therefore, not hydrogen bonded. Using transient absorption spectroscopy (TAS), we measure the lifetime of hot electrons photoexcited in plasmon resonant nanostructures.5 Using transient reflectance spectroscopy (TRS), we measure the photoexcited carrier dynamics in a GaP/TiO2 photoelectrode, as well as the electrostatic field dynamics at this semiconductor-liquid interfaces in situ under various electrochemical potentials.4 Here, the electrostatic fields at the surface of the semiconductor are measured via Franz−Keldysh oscillations (FKO). These spectra reveal that the nanoscale TiO2 protection layer enhances the built-in field and charge separation performance of GaP photoelectrodes. Shi, H.T., B.F. Zhao, J. Ma, M.J. Bronson, Z. Cai, J.H. Chen, Y. Wang, M. Cronin, L. Jensen and S.B. Cronin, Measuring Local Electric Fields and Local Charge Densities at Electrode Surfaces Using Graphene-Enhanced Raman Spectroscopy (GERS)-Based Stark-Shifts. ACS Applied Materials & Interfaces, 11, 36252-36258 (2019). Chen, J., C.S. Bailey, D. Cui, Y. Wang, B. Wang, H. Shi, Z. Cai, E. Pop, C. Zhou and S.B. Cronin, Stacking Independence and Resonant Interlayer Excitation of Monolayer WSe2/MoSe2 Heterostructures for Photocatalytic Energy Conversion. ACS Applied Nano Materials, DOI:10.1021/acsanm.9b01898 (2020). Montenegro, A., C. Dutta, M. Mammetkuliev, H.T. Shi, B.Y. Hou, D. Bhattacharyya, B.F. Zhao, S.B. Cronin and A.V. Benderskii, Asymmetric response of interfacial water to applied electric fields. Nature, 594, 62 (2021). Xu, Z.H., B.Y. Hou, F.Y. Zhao, Z. Cai, H.T. Shi, Y.W. Liu, C.L. Hill, D.G. Musaev, M. Mecklenburg, S.B. Cronin and T.Q. Lian, Nanoscale TiO2 Protection Layer Enhances the Built-In Field and Charge Separation Performance of GaP Photoelectrodes. Nano Letters, 21, 8017-8024 (2021). Yu Wang, Yi Wang, Indu Aravind, Zhi Cai, Lang Shen, Boxin Zhang, Bo Wang, Jihan Chen, Bofan Zhao, Haotian Shi, Jahan M. Dawlaty, and Stephen B. Cronin. In Situ Investigation of Ultrafast Dynamics of Hot Electron-Driven Photocatalysis in Plasmon-Resonant Grating Structures. Journal of the American Chemical Society. DOI: 10.1021/jacs.1c12069 (2022). Haotian Shi, Ryan T. Pekarek, Ran Chen, Boxin Zhang, Yu Wang, Indu Aravind, Zhi Cai, Lasse Jensen, Nathan R. Neale, and Stephen B. Cronin. Monitoring Local Electric Fields using Stark Shifts on Napthyl Nitrile-Functionalized Silicon Photoelectrodes. The Journal of Physical Chemistry C, 124, 17000-17005 (2020).

Nucleases are family of hydrolytic enzymes which cleave the phosphodiesteric bonds between the nucleotides producing 3'-phospho or 5'-phospho end products. These enzymes can be classified as endo- and exo-nucleases, cleaving inside of a polynucleotide chain or at the ends respectively, however, some enzymes may have both activities. Typically, some nucleases prefer double-stranded nucleic acid (e.g. DNase I), while other cleave single-stranded (e.g. Nuclease P1) or RNA-DNA hybrid (RNase H) polynucleotides. Deoxyribonucleases (DNases) are important objects in biotechnology presenting both as tools for manipulations with DNA1 as well as contaminants where nuclease-like activity is undesired.2 In addition, data emerges indicating the importance of DNase activity detection for medical applications, i.e., DNases may be related with various diseases and could be used to monitor the progress of various cancers3 , 4 as well as could be a rapid and fast biomarker of bacterial infections.5 Typically, DNases are being detected and quantified using classical approach: fluorescence detection or gel electrophoresis techniques. Though those methods are highly sensitive and reliable, they are costly, time-consuming as well as require toxic markers such as ethidium bromide or radiolabeled molecules. As alternative methods novel detection techniques for DNase detection were reported, mostly based on fluorescence or chemiluminescence detection incorporating nanomaterials for signal enhancement.6 In addition, electrochemical biosensors, provide simple and inexpensive platform for biomolecule detection while maintaining a high degree of accuracy and sensitivity. However, to this day the absolute majority of electrochemical biosensors are being developed from small biomolecules, e.g., glucose, glycerol, lactate while the development of large marcomolecules, i.e., proteins or enzymes is still lagging. In a case of DNase detection, a few biosensors have been reported. Sato et al. reported electrochemical biosensor for DNase I detection based on ferrocene-modified DNA immobilized on the electrode surface with the detection limit (LOD) up to 10–2 U mL–1. Ding and Qin have reported a potentiometric biosensor for the detection of DNAses based on polycation-sensitive membrane, and for DNase I the LOD was 0.45 U mL–1.7 Despite advances, the developed electrochemical biosensors for DNase detection still lack sensitivities comparable to fluorescence- or radiolabeling-based detection methods and reliability required for the analysis of real samples. In this work we present a rapid and sensitive bioelectrochemical device for the determination of nuclease activity in various fluids. The device system consists of a sensor electrode, a special design DNA target to maximize the nuclease cleavage rate, signal analysis algorithm and the supporting electronics. The developed sensor allows to determine DNase activity in range 0.0003–0.03 U mL–1 with the limit of detection up to 0.00034 U mL–1 over 15 min measurement (calibrated against DNase I standard) as well as has a high storage stability. The system was also implemented using inexpensive single-used electrodes. Moreover, the sensor was tested measuring nuclease activity in real unmodified human saliva samples and demonstrated high accuracy compared to the industry standard DNaseAlert™ QC System. The developed technology could significantly improve nuclease quality control processes in pharma/biotech industry and give new insights into nuclease importance for medical applications. References (1) Pan, Y.; Xiao, L.; Li, A. S. S.; Zhang, X.; Sirois, P.; Zhang, J.; Li, K. Mol Biotechnol 2013, 55 (1), 54–62. (2) Senavirathne, G.; Liu, J.; Lopez, M. A.; Hanne, J.; Martin-Lopez, J.; Lee, J.-B.; Yoder, K. E.; Fishel, R. Nat Methods 2015, 12 (10), 901–902. (3) Patel, P. S.; Patel, B. P.; Rawal, R. M.; Raval, G. N.; Patel, M. M.; Patel, J. B.; Jha, F. P.; Patel, D. D. Tumor Biol 2000, 21(2), 82–89. (4) Balian, A.; Hernandez, F. J. Biomark Res 2021, 9 (1), 86. (5) Flenker, K. S.; Burghardt, E. L.; Dutta, N.; Burns, W. J.; Grover, J. M.; Kenkel, E. J.; Weaver, T. M.; Mills, J.; Kim, H.; Huang, L.; Owczarzy, R.; Musselman, C. A.; Behlke, M. A.; Ford, B.; McNamara, J. O. Molecular Therapy 2017, 25 (6), 1353–1362. (6) Mozioğlu, E.; Akgoz, M.; Kocagöz, T.; Tamerler, C. Anal. Methods 2016, 8 (20), 4017–4021. (7) Ding, J.; Qin, W. Biosensors and Bioelectronics 2013, 47, 559–565. Figure. The general operation scheme of nuclease biosensor. (A) In a case a sample does not contain nuclease the Target nucleotide is not cleaved and its hybridization rate is high. (B) In a case sample contains nucleases the Target nucleotide is cleaved and its hybridization rate is lower and relates to the nuclease activity. Figure 1

Abstract Background: Multiple Myeloma (MM) is a hematological malignancy characterized by abnormal proliferation of terminally differentiated plasma cells (PCs) in the bone marrow (BM). MM is almost always preceded by the precursor stage smoldering multiple myeloma (SMM). BM biopsies are useful to monitor disease progression, but they are invasive and not routinely collected from patients for disease monitoring during precursor stages. Profiling circulating tumor cells (CTCs) from peripheral blood (PB) could aid early detection, disease monitoring, and biomarker identification to predict patients at high risk of progression that may benefit from early therapeutic intervention. Methods: Paired PB and BM aspirates were collected from 40 SMM patients enrolled in the PCROWD study (IRB #14-174) at Dana-Farber Cancer Institute. Malignant PCs were enriched by magnetic bead-based methods and underwent 5’ single-cell RNA sequencing (scRNA-seq) and single-cell B-cell receptor sequencing (scBCR-seq) (10x Genomics). Results: We analyzed 105,246 BM PCs and 33,234 PB PCs from 15 patients. To differentiate malignant from normal PCs, we used clonal V(D)J rearrangements, assessed by concurrent scBCR-seq. A total of 86,986 BM tumor cells and 8,718 CTCs were captured. A median of 5, 26, and 47 CTCs were present per mL of blood from low, intermediate, and high-risk SMM patients as defined by the International Myeloma Working Group (IMWG) “20/2/20” criteria, suggesting sequencing-based CTC enumeration corresponds to prognosis. High levels of driver genes commonly upregulated in patients with specific translocations, including CCND1 and MAF, were detected in both BM tumor and CTC clusters in 3 patients with t(11;14) and t(14;16) confirmed by fluorescence in situ hybridization (FISH) clinical testing, and 2 additional patients with inconclusive FISH results (Wilcoxon, q <10-3), supporting the idea of CTC-based prognostication. Differential expression (DE) analysis revealed 8 genes that were significantly upregulated and 3 genes that were significantly downregulated in CTCs compared to BM tumor cells robustly across 15 paired samples. Gene set enrichment analysis (GSEA) revealed genes DE in CTCs are associated with TNF-α and NF-κB signaling, which are commonly induced by extrinsic factors in the bone marrow milieu, providing insight into the biology of tumor cell circulation. Conclusions: This study highlights the utility of scRNA-seq for molecular profiling of CTCs, even in asymptomatic low tumor burden disease. Additional analyses are ongoing in the expanded cohort of 40 patients with paired samples to help gain further insight into CTC heterogeneity. Overall, this study will help enable the design of new molecular liquid biopsy-based approaches to diagnosis, disease monitoring, and biological insights to improve treatment strategies for precursor myeloma patients. Citation Format: Elizabeth D. Lightbody, Danielle T. Firer, Romanos Sklavenitis-Pistofidis, Michael Agius, Ankit K. Dutta, Michelle Aranha, Jean-Baptiste Alberge, Laura Hevenor, Nang Kham Su, Cody Boehner, Erica Horowitz, Jacqueline Perry, Anna Cowan, Hadley Barr, Anna Justis, Daniel Auclair, Catherine R. Marinac, Gad Getz, Irene Ghobrial. Single-cell RNA sequencing of rare circulating tumor cells in precursor myeloma patients reveals molecular underpinnings of tumor cell circulation [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 641.

<b>Rechtsschutz und Verfahren</b> sind zentrale Bausteine der unionalen Rechtsordnung. Nur derjenige, der die strukturellen Parallelen und Gemeinsamkeiten der verschiedenen Verfahrensordnungen kennt, kann konkrete Lösungen für unionsrechtlich gelagerte Fälle anbieten. <b>Das Handbuch zum EU-Rechtsschutz</b> entwickelt diese notwendige Gesamtperspektive. Aus einer Hand werden sämtliche wichtigen Rechtsschutzoptionen und Verfahrensarten vor dem EuGH, dem EUGMR sowie aus den Bereichen des europäischen Zivil-, Verwaltungs- und Strafverfahrensrechts dargestellt und in übergeordnete Aspekte der unionalen Verfahrens- und Rechtsschutzidee eingebettet. Besonders berücksichtigt sind dabei das Vorabentscheidungs- und Vertragsverletzungsverfahren sowie die Untätigkeits-, Nichtigkeits- und Schadensersatzklage. <b>Die 2. Auflage</b> vermittelt dem nationalen Rechtsanwender in Justiz und Anwaltschaft auf <b>neuestem Stand</b> eine klare Orientierungshilfe im Geflecht der konkreten Rechtsschutzoptionen im Zivil-, Verwaltungs- und Strafrecht. <b>Schwerpunkte</b> der Neuauflage liegen auf den Bereichen Anerkennung und Vollstreckung von Entscheidungen in Zivil-, Handels-, Ehe-, Unterhalts-, Kindschafts- und Erbsachen sowie dem Europäischen Nachlasszeugnis. Europäische Bagatell- und Mahnverfahren und Vollstreckungstitel; Zwangsvollstreckung Insolvenzverfahren Beweisaufnahme, Zustellung , Prozesskostenhilfe, Unterlassungsklagen Alternative Streitbeilegung und Schiedsverfahren Rechtsschutz im europäischen Verwaltungsrecht Rechtsschutz im europäischen Strafrecht Verfahren der strafjustiziellen Zusammenarbeit <b>Die Autorinnen und Autoren</b> Prof. Dr. Tilmann Altwicker; Prof. Dr. Dr. Ino Augsberg; Prof. Dr. Steffen Augsberg; Prof. Dr. Jürgen Bast; Prof. Dr. Anatol Dutta; Prof. Dr. Michael Fehling; Prof. Dr. Claudio Franzius; Prof. Dr. Jörg Gundel; Prof. Dr. Wolfgang Hau; Prof. Dr. Steffen Hindelang; Jun.-Prof. Dr. Suzan Denise Hüttemann; Duy Tuong Huynh; Malte Ising; Prof. Dr. Ruth Janal; Barrister (QLD, adm) Thomas John ACIArb; RA Dr. Ulrich Karpenstein; Dr. Rainer Kemper; RA Dr. Sebastian Koehler; Dr. Christian Koller; Prof. Dr. Markus Kotzur; Dr. Pia Lange; Prof. Dr. Matthias Lehmann; Prof. Dr. Stefan Leible; Prof. Dr. Eva Lein; Prof. Dr. Katharina Lugani; Prof. Dr. Peter Mankowski; Prof. em. Dr. Dieter Martiny; Prof. Dr. Frank Meyer; PD Dr. Michael F. Müller; Prof. Dr. Carsten Nowak; Prof. DDr. h. c.Paul Oberhammer; Prof. Dr. Anne Peters; Prof. Dr. Dr. h.c. Walter H. Rechberger; Prof. Dr. Herbert Roth; Dr. Jakob Schemmel; RiLG Michael Slonina; Prof. Dr. Astrid Stadler; Prof. Dr. Jürgen Stamm; Prof. Dr. Michael Stürner; Prof. Dr. Jörg Philipp Terhechte; PD Dr. Alexander Thiele; Prof. Dr. Christoph Thole; PräsBVerfG a. D. Prof. Dr. Dres. h.c. Andreas Voßkuhle; RA Bertrand Wägenbaur; Dr. Felix M. Wilke; Dipl. Rpfl. Harald Wilsch; Prof. Dr. Hinnerk Wißmann