Auswahl der wissenschaftlichen Literatur zum Thema „Cymbidium rot“

Dendrobium officinale is a medicinal herbal plant with important health care value and high demand. Due to its slow growth and scarcity in nature, its yield depends on intensified cultivation while biotic and abiotic stresses were important factors that causes production loss. Orchidaceae can form association with rhizoctonias collectively, and studies have found that some orchids showed a high level of strain-species specificity to orchid mycorrhizal fungi (OMF), yet the specificity of OMF on D. officinale needs to explored. In this study, the effects on D. officinale of four OMF isolated from Cymbidium were tested. The obviously higher mass yield of the treated plants in medium and pots indicated the growth promotion effect of the fungi. Furthermore, an abiotic stress test indicated stronger drought tolerance among the treated plants. For the biotic stress test, two root rot pathogens, Fusarium solani and Fusarium graminearum , were isolated and identified from root rot of D. officinale. In an in vitro inhibition test, the four OMF could resist the growth of these pathogens. In vivo studies showed that these four OMF could improve the survival rate and fresh weight and decrease the root rot rate of pathogen-inoculated seedlings. The four OMF namely; Hyphomycete sp., Umbelopsis sp., Ceratorhiza sp. and Ceratorhiza sp. are compatible strains for improving the growth rate of D. officinale by increasing its environmental stress tolerance, providing an effective way to supply resources through artificial reproduction.

Cymbidium spp. is an orchid of great horticultural value cultivated extensively in Eastern Himalaya, India. Since 1995, growers have experienced huge crop losses in every monsoon month because of pseudobulb rot. Pseudobulbs initially turned soft and pulpy followed by oozing of a dark brown liquid with a foul odor (early phase). With increasing severity, the bulbs and roots lose weight as the internal tissues gradually disintegrate (middle phase). Finally, the bulb becomes hollow, fibrous, and dry causing death of the plant (later phase). Surveys from 2002 to 2005 showed that disease incidence ranged from 60 to 100%. Rotted tissue was plated on nutrient agar and potato dextrose agar media. Three organisms were consistently isolated from 50 samples collected from 30 different localities. They were identified as Erwinia carotovora (2), Fusarium oxysporum (3), and Mucor hiemalis f. sp. hiemalis (1) and were predominant at the earlier, middle, and later stages of disease, respectively. Identifications were further confirmed by the Agricultural Research Institute (ARI), Pune, India. Pseudobulbs were surface sterilized with 0.5% sodium hypochlorite for 1 min, washed by sterile distilled water, and dipped separately into three different spore/cell suspensions (105 CFU/ml) for 1 min. Another set of sterilized bulbs was dipped first into E. carotovora, then into F. oxysporum 12 days later, and then into M. hiemalis f. sp. hiemalis 15 days after the second dip. For the control set, bulbs were dipped into sterile distilled water. Samples were incubated aseptically at 20°C with a relative humidity of 80%, and all inoculated bulbs were evaluated for disease 47 days after the first inoculation. When samples were inoculated separately, E. carotovora exhibited maximum (70%) tissue disintegration followed by F. oxysporum (30%) and M. hiemalis f. sp. hiemalis (10%), but none of the individual pathogens caused 100% tissue disintegration. Complete destruction was observed after 47 days of first inoculation when these three pathogens were inoculated consecutively according to their serial occurrence. It is an interesting report on host-pathogen combination as three pathogens act in sequence toward ultimate demolition of the host. We report this rot as a synergistic activity of three pathogens to cause an uncontrolled epidemic disease of Cymbidium spp. References: (1) J. C. Gilman. Page 37 in: A Manual of Soil Fungi. Iowa State College Press. Ames, IA, 1945. (2) J. G. Holt. Page 469 in: Bergey's Manual of Systematic Bacteriology. Vol. I. Williams and Wilkins. Baltimore/London, 1984, (3) C. V. Subramaniam. Page 657 in: Hyphomycetes. Indian Council of Agricultural Research (ICAR). New Delhi, 1971.