Thematische Bibliographien / CyclinB

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Auswahl der wissenschaftlichen Literatur zum Thema „CyclinB“

Autor: Grafiati

Veröffentlicht am 25. Mai 2024

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Zeitschriftenartikel zum Thema "CyclinB"

Yi, Xie, und Li Bing. „The Transcription Express Characteristics of Several Genes in the Process of Bombyx mori Ovarian Carcinoma“. Advanced Materials Research 796 (September 2013): 39–42. http://dx.doi.org/10.4028/www.scientific.net/amr.796.39.

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Bombyx mori cell line (BmN) comes fromBombyx moriovary cell subculture. In order to study the change of several genes transcription in the process ofBombyx moriovary cells primary culture and subculture, we usedBombyx moriovary organizations and BmN cell lines as research materials, used Real Time fluorescent quantitative RT-PCR to detect cyclin gene family (CyclinA, CyclinB, CyclinB3, CyclinE, CyclinL1), p53 and Telomerase genes transcription level in the ovary and BmN cell lines, and took Actin3 gene as reference to dispose the results. The results showed that in theBombyx moriBmN cell lines the expression of CyclinA, CyclinB, CyclinB3, CyclinE, CyclinL1 and Telomerase genes were higher than those in the ovary. The expression of CyclinB in the BmN was more then 3.8 which was 76 times higher than that in the ovary; The expression of p53 gene in the BmN cell was lower than that in the ovary; The expression of Telomerase gene in the BmN cell was higher than that in the ovary. The results accumulated a reliable data for further study on the the role of cyclin gene family, p53 gene, and Telomerase gene in the process ofBombyx moriovarian carcinoma.

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Buchkovich, K. J., und E. B. Ziff. „Nerve growth factor regulates the expression and activity of p33cdk2 and p34cdc2 kinases in PC12 pheochromocytoma cells.“ Molecular Biology of the Cell 5, Nr. 11 (November 1994): 1225–41. http://dx.doi.org/10.1091/mbc.5.11.1225.

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In the absence of serum, nerve growth factor (NGF) promotes the survival and differentiation of the PC12 pheochromocytoma cell line. In the presence of serum, NGF acts primarily as a differentiation factor and negative regulator of cell cycling. To investigate NGF control of cell cycling, we have analyzed the regulation of cyclin dependent kinases during PC12 cell differentiation. NGF treatment leads to a reduction in the steady-state protein levels of p33cdk2 and p34cdc2, two key regulators of cell cycle progression. The decrease in p33cdk2 and p34cdc2 coincides with a decrease in the enzymatic activity of cyclinA-p34cdc2, cyclinB-p34cdc2, cyclinE-p33cdk2, and cyclinA-p33cdk2 kinases. The decline in p33cdk2 and p34cdc2 kinase activity in response to NGF is accelerated in cells that over-express the p140trk NGF receptor, suggesting that the timing of the down- regulation is dependent on the level of p140trk and the strength of the NGF signal. The level of cyclin A, a regulatory subunit of p33cdk2 and p34cdc2, is relatively constant during PC12 differentiation. Nevertheless, the DNA binding activity of the cyclinA-associated transcription factor E2F/DP decreases. Thus, NGF down-regulates the activity of cyclin dependent kinases and cyclin-transcription factor complexes during PC12 differentiation.

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Ehrhardt, Harald, Catarina Castro Alves, Franziska Wachter und Irmela Jeremias. „TRAIL Preferentially Affects Cell Cycle-Arrested Tumor Cells Including Stem- and Progenitor Cells From Patients with Acute Lymphoblastic Leukemia“. Blood 120, Nr. 21 (16.11.2012): 1879. http://dx.doi.org/10.1182/blood.v120.21.1879.1879.

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Abstract Abstract 1879 Leukemic stem- and progenitor cells exhibit low cycling activity which might represent a major cause for their increased treatment resistance. TRAIL (TNF-related apoptosis inducing ligand) is a novel putative anticancer drug currently in phase I and II clinical testing. We recently showed that TRAIL is able to address stem- and progenitor cells from patients with acute lymphoblastic leukemia (ALL) in xenotransplantation assays (Alves et al., Blood 2012,119,4224). As stem- and progenitor cells are often non-cycling, we asked here, whether TRAIL is able to address resting leukemia cells. We used cell lines and primary tumor cells from children with ALL which were amplified in severely immuno-compromised mice (NSG mice). Cell cycle arrest was induced (i) by addition of conventional cytotoxic drugs which are known to act as cytostatic drugs such as doxorubicine; (ii) by biochemical inhibitors known to induce cell cycle arrest at different defined points of the cell cycle such as mimosine; (iii) by molecular approaches and knockdown of cyclinB arresting cell cycle in G2 or knockdown of cyclinE arresting cell cycle in G1. Unexpectedly, TRAIL-induced apoptosis was enhanced, whenever cell cycle was arrested. Cell cycle arrest sensitized towards TRAIL-induced apoptosis independently from the point or phase of cell cycle which was arrested (G0, G1 or G2) and independently from the agent used to arrest the cell cycle. Similarly, knockdown of cyclinB or cyclinE both clearly sensitized cell line cells towards TRAIL-induced apoptosis. Cytotoxic drugs and cell cycle inhibitors might arrest the cell cycle by activation of p53. Accordingly, when caffeine was added which inhibited p53 activity and drug-induced cell cycle arrest, sensitization towards TRAIL-induced apoptosis was blocked. We have recently established a novel method which enables performing knockdown experiments in tumor cells derived from ALL patients (Höfig et al., Cell Comm. Signal. 2012,10,8). Using this method and most important for clinical translation, we could show that knockdown of either cyclinB or cyclinE clearly sensitized patient-derived ALL cells towards TRAIL-induced apoptosis. Taken together and in contrast to most conventional cytotoxic drugs, TRAIL exerts anti-tumor activity preferentially against tumor cells in cell cycle arrest and less against actively cycling tumor cells. This special feature of TRAIL might explain its anti-tumor activity against stem- and progenitor cells in patients with ALL. Thus, TRAIL might represent an interesting drug to treat disease stages with accumulation of stem- and progenitor cells and static tumor disease, e.g., during minimal residual disease. Disclosures: No relevant conflicts of interest to declare.

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Kannan, Sankaranarayanan, Wendy Fang und Patrick Zweidler-McKay. „Multiple Mechanisms of Notch-Mediated B-ALL Growth Arrest Via HES1 and PLK1: KIF11, CyclinB and p53“. Blood 116, Nr. 21 (19.11.2010): 3137. http://dx.doi.org/10.1182/blood.v116.21.3137.3137.

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Abstract Abstract 3137 Notch signaling inhibits B-ALL growth and survival, while it promotes T-ALL, revealing contrasting cell-specific consequences of Notch signaling in ALL subtypes. We previously reported that HES1 (Hairy and Enhancer of split1) is a Notch pathway member which is sufficient to reproduce these effects in B-ALL. We have found distinct HES1 protein complexes in B-ALL versus T-ALL, with significantly higher molecular weight complexes found in B-ALL. Through characterization of these complexes, multiple novel HES1 interacting proteins have been identified which regulate HES1 function as well as provide growth inhibiting and pro-apoptotic effects. We previously reported the novel role of HES1:PARP1 complexes in inducing B-ALL apoptosis. This study reports Polo-like kinase1 (PLK1) and Kinesin Family member 11 (KIF11, also known as Eg5) as novel HES1 interacting proteins in B-ALL. PLK1 is highly expressed in B cell leukemias and drives multiple proliferative pathways including activation of KIF11, induction of cyclinB and degradation of p53. KIF11 is also highly expressed in B-ALL and promotes cell cycle progression through enhanced spindle formation, while cyclinB promotes accelerated mitosis. Through HES1 immunoprecipitation and MALDI-TOF peptide analysis, we identified KIF11 as a potential HES1 interacting protein. Low flow size exclusion chromatography revealed that HES1 and KIF11 co-migrate in high molecular weight complexes. Reciprocal immunoprecipitation (IP) with HES1 and KIF11 antibodies reveal a strong protein-protein interaction in B-ALL cells. PLK1 was also identified in these complexes, revealing a novel interaction of HES1, KIF11 and PLK1. Importantly, induction of Notch signaling via HES1 in B-ALL decreases both PLK1 and KIF11 levels, associated with G1 cell cycle arrest. Furthermore, HES1 expression decreased cyclinB protein levels, but not cyclin A/E, consistent with inhibition of PLK1. Finally, in the same model we observe increased p53 and p21WAF1 protein levels, also contributing to growth arrest. These data reveal that multiple mechanisms of Notch/HES1-mediated growth arrest may occur via PLK1 in B-ALL. The novel interaction of HES1 with PLK1 and KIF11 and resulting effects on KIF11, cyclinB and p53/p21 pathways may represent important mechanisms of Notch-mediated growth inhibition in B-ALL. Disclosures: No relevant conflicts of interest to declare.

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Lammer, C., S. Wagerer, R. Saffrich, D. Mertens, W. Ansorge und I. Hoffmann. „The cdc25B phosphatase is essential for the G2/M phase transition in human cells“. Journal of Cell Science 111, Nr. 16 (15.08.1998): 2445–53. http://dx.doi.org/10.1242/jcs.111.16.2445.

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Cdc25 phosphatases play key roles in cell cycle progression by activating cyclin-dependent kinases. In human cells, cdc25 proteins are encoded by a multigene family, consisting of cdc25A, cdc25B and cdc25C. While cdc25A plays a crucial role at the G1/S phase transition, cdc25C is involved in the dephosphorylation and activation of the mitotic kinase, cdc2/cyclinB. In addition, cdc25C itself is regulated by cdc2/cyclinB which then creates a positive feedback loop that controls entry into mitosis. In this study we show that the activity of cdc25B appears during late S phase and peaks during G2 phase. Both in vitro and in vivo cdc25B is activated through phosphorylation during S-phase. Using a cell duplication, microinjection assay we show that ablation of cdc25B function by specific antibodies blocks cell cycle progression in Hs68 cells by inhibition of entry into mitosis. Cdc25B function neither plays a role in later stages of mitosis nor for the inititation of DNA replication. These results indicate that cdc25B is a mitotic regulator that might act as a ‘starter phosphatase’ to initiate the positive feedback loop at the entry into M phase.

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Chiroli, Elena, Roberta Fraschini, Alessia Beretta, Mariagrazia Tonelli, Giovanna Lucchini und Simonetta Piatti. „Budding yeast PAK kinases regulate mitotic exit by two different mechanisms“. Journal of Cell Biology 160, Nr. 6 (17.03.2003): 857–74. http://dx.doi.org/10.1083/jcb.200209097.

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We report the characterization of the dominant-negative CLA4t allele of the budding yeast CLA4 gene, encoding a member of the p21-activated kinase (PAK) family of protein kinases, which, together with its homologue STE20, plays an essential role in promoting budding and cytokinesis. Overproduction of the Cla4t protein likely inhibits both endogenous Cla4 and Ste20 and causes a delay in the onset of anaphase that correlates with inactivation of Cdc20/anaphase-promoting complex (APC)–dependent proteolysis of both the cyclinB Clb2 and securin. Although the precise mechanism of APC inhibition by Cla4t remains to be elucidated, our results suggest that Cla4 and Ste20 may regulate the first wave of cyclinB proteolysis mediated by Cdc20/APC, which has been shown to be crucial for activation of the mitotic exit network (MEN). We show that the Cdk1-inhibitory kinase Swe1 is required for the Cla4t-dependent delay in cell cycle progression, suggesting that it might be required to prevent full Cdc20/APC and MEN activation. In addition, inhibition of PAK kinases by Cla4t prevents mitotic exit also by a Swe1-independent mechanism impinging directly on the MEN activator Tem1.

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Ji, J. Y. „Genetic interactions between Cdk1-CyclinB and the Separase complex in Drosophila“. Development 132, Nr. 8 (15.04.2005): 1875–84. http://dx.doi.org/10.1242/dev.01780.

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Margolis, Seth S., Jennifer A. Perry, Douglas H. Weitzel, Christopher D. Freel, Minoru Yoshida, Timothy A. Haystead und Sally Kornbluth. „A Role for PP1 in the Cdc2/Cyclin B–mediated Positive Feedback Activation of Cdc25“. Molecular Biology of the Cell 17, Nr. 4 (April 2006): 1779–89. http://dx.doi.org/10.1091/mbc.e05-08-0751.

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The Cdc25 phosphatase promotes entry into mitosis through the removal of inhibitory phosphorylations on the Cdc2 subunit of the Cdc2/CyclinB complex. During interphase, or after DNA damage, Cdc25 is suppressed by phosphorylation at Ser287 (Xenopus numbering; Ser216 of human Cdc25C) and subsequent binding of the small acidic protein, 14-3-3. As reported recently, at the time of mitotic entry, 14-3-3 protein is removed from Cdc25 and S287 is dephosphorylated by protein phosphatase 1 (PP1). After the initial activation of Cdc25 and consequent derepression of Cdc2/CyclinB, Cdc25 is further activated through a Cdc2-catalyzed positive feedback loop. Although the existence of such a loop has been appreciated for some time, the molecular mechanism for this activation has not been described. We report here that phosphorylation of S285 by Cdc2 greatly enhances recruitment of PP1 to Cdc25, thereby accelerating S287 dephosphorylation and mitotic entry. Moreover, we show that two other previously reported sites of Cdc2-catalyzed phosphorylation on Cdc25 are required for maximal biological activity of Cdc25, but they do not contribute to PP1 regulation and do not act solely through controlling S287 phosphorylation. Therefore, multiple mechanisms, including enhanced recruitment of PP1, are used to promote full activation of Cdc25 at the time of mitotic entry.

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Mora, Catia Celeste, María Florencia Perotti, Eduardo González-Grandío, Pamela Anahí Ribone, Pilar Cubas und Raquel Lía Chan. „AtHB40 modulates primary root length and gravitropism involving CYCLINB and auxin transporters“. Plant Science 324 (November 2022): 111421. http://dx.doi.org/10.1016/j.plantsci.2022.111421.

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Ren, Liping, Anna Feoktistova, W. Hayes McDonald, Greg Den Haese, Jennifer L. Morrell und Kathleen L. Gould. „Analysis of the Role of Phosphorylation in Fission Yeast Cdc13p/CyclinB Function“. Journal of Biological Chemistry 280, Nr. 15 (10.02.2005): 14591–96. http://dx.doi.org/10.1074/jbc.m500560200.

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Dissertationen zum Thema "CyclinB"

Ewert-Krzemieniewska, Katarzyna. „Investigation into the regulation of DNA repair by the S. pombe cell cycle kinase Cdc2-cyclinB“. Thesis, Bangor University, 2009. https://research.bangor.ac.uk/portal/en/theses/investigation-into-the-regulation-of-dna-repair-by-the-spombe-cell-cycle-kinase-cdc2cyclinb(71f3241d-b969-4ff1-9b16-47161f91b755).html.

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The work presented in this thesis proposes two novel functions for S. pombe Cdc2 in the cell cycle-dependent coordination of DNA recombination: (i) during unperturbed cell cycle and (ii) in response to camptothecin (CPT)-induced DNA breaks. In unperturbed cells, in vivo elevated Cdc2 activity causes problems during DNA replication that lead to an increase in spontaneous gene conversion between sister chromatids and enhanced loss of a non-essential minichromosome. Data presented here suggest that Cdc2 regulates the anti-recombinogenic activity of the Srs2 DNA helicase to prevent such spontaneous gene conversion events in S phase. Both proteins associate with PCNA in distinct protein complexes, which may allow them to regulate DNA repair in S phase. Elevated Cdc2 activity leads to constitutive phosphorylation of the checkpoint kinase Chk1, indicating that the inability to regulate Srs2 DNA helicase causes DNA replication lesions, which engage the G2-M checkpoint. Cells with elevated Cdc2 activity are specifically sensitive to the Topoisomerase I (Top1) poison CPT. The camptothecin sensitivity of cdc2.1w mutant cells increases in the absence of Tyrosyl-DNA-phosphodiesterase (Tdp1), which cleaves immobilised Top1 releasing it from the 3'-end of DNA in S phase. As during the unperturbed cell cycle, Cdc2 appears to regulate Srs2 DNA helicase under these circumstances. Srs2 may unwind the blocked 3'-strand in the absence of Tdp1 to allow a nuclease to access the damaged site. Both Mus81 and Rad16 are potential candidates since both nucleases act in the Cdc2-dependent pathway in response to CPT. Although all "wee" mutants with elevated Cdc2 activity are defective in this CPT repair pathway, both Wee1 and Mik1 kinase may perform independent repair functions.

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Bockstaele, Laurence. „Réévaluation de la régulation de l'activité de la CDK4, kinase dépendante des cyclines D, clé de l'engagement dans le cycle cellulaire: rôle de l'inhibiteur p27“. Doctoral thesis, Universite Libre de Bruxelles, 2006. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210626.

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La progression à travers le cycle cellulaire est assurée par l’activation séquentielle d’une série de complexes cycline-CDK. Les complexes cycline D-CDK4 assurent la progression au cours de la phase G1 du cycle cellulaire en phosphorylant les protéines « antioncogéniques » de la famille Rb. L’activation de la CDK4 nécessite son association à une cycline D et sa phosphorylation sur Thr172 par la CAK nucléaire (CDK activating kinase). Le rôle essentiel des protéines de la famille Cip/Kip dans la régulation de l’activité de ces complexes reste controversé. Les protéines de cette famille (comprenant p27 et p21) ont initialement été identifiées comme des inhibiteurs puissants des complexes cycline-CDK et comme les intermédiaires de l’action antimitogénique de différents signaux intra ou extra-cellulaires. Il a été proposé que la liaison de la p27 ou de la p21 aux complexes cycline D-CDK4 empêche leur phosphorylation activatrice par la CAK et leur activité pRb kinase. Cependant, l’observation que des complexes cycline D-CDK4 associés à p21/p27 possèdent une activité pRb kinase a donné naissance à une seconde hypothèse sur la régulation de ces complexes par la p27 ou la p21. Ces « inhibiteurs » ont été paradoxalement proposés comme facteurs nécessaires et suffisants d’assemblage et de localisation nucléaire des complexes cycline D-CDK4. Dans le modèle physiologiquement relevant des thyrocytes de chien en culture primaire, la mitogénèse dépendante de l’AMPc activée par la TSH diffère des cascades des facteurs de croissance puisqu’elle n’induit pas les cyclines D mais au contraire augmente l’accumulation de l’«inhibiteur» de CDK p27. L’AMPc stimule l’assemblage des complexes cycline D3-CDK4, leur translocation nucléaire et leur association à p27. Dans ce modèle, le TGF&61538; inhibe la mitogénèse dépendante de l’AMPc en inhibant la translocation nucléaire des complexes cycline D3-CDK4 et leur association à la p27.

Nous avons étudié l’activité catalytique et l’activation des complexes cycline D3-CDK4-p27 issus des thyrocytes de chien en culture primaire ou produits en cellules d’eucaryotes supérieurs (CHO et Sf9). Nous avons pu montrer que les complexes cycline D3-CDK4-p27 issus des thyrocytes de chien stimulés par la TSH présentent une activité pRb-kinase qui est inhibée par le TGF&61538; En outre, la production des complexes cycline D3-CDK4-p27 en cellules CHO ou Sf9 nous a permis de montrer que l’impact de la p27 sur l’activité catalytique des complexes cycline D3-CDK4 dépend de sa stoechiométrie de liaison à ces complexes. L’analyse du profil de séparation par électrophorèse bidimensionnelle de la CDK4 issue de ces trois systèmes montre que la p27 n’empêche pas la phosphorylation activatrice de la CDK4, même aux concentrations de p27 qui empêchent l’activité pRb kinase du complexe cycline D3-CDK4. Nous avons également montré dans les cellules CHO que la p27 détermine la localisation nucléaire des complexes cycline D3-CDK4, ceux-ci étant relocalisés dans le cytoplasme par la transfection d’un mutant de la p27 dépourvu de son signal de localisation nucléaire. Ces résultats valident les observations réalisées par immunofluorescence dans les thyrocytes de chien dans lesquels nous avons mis en évidence une étroite corrélation au niveau des cellules individuelles stimulées par la TSH entre la translocation nucléaire de la CDK4 et l’apparition de la p27 nucléaire. Cette colocalisation est partiellement inhibée par le TGF&61538; Ces observations renforcent l’hypothèse d’un rôle de la p27 dans l’ancrage nucléaire des complexes cycline D3-CDK4.

Alors que la localisation de la CAK est considérée comme exclusivement nucléaire et son activité catalytique constitutive, nous avons pu montrer que la phosphorylation activatrice de la CDK4 associée à la cycline D3 n’est pas affectée par sa localisation sub-cellulaire et qu’elle est régulée par le TGF&61538; dans les thyrocytes de chien et par le sérum dans les cellules T98G indépendamment de l’association de la CDK4 à la p27. De plus, la phosphorylation de la CDK4 sur Thr172 dans les cellules T98G est stimulée par le sérum, alors que la phosphorylation activatrice de la CDK6, son homologue fonctionnel, ne l’est pas. La comparaison de la séquence de ces deux CDKs à proximité des Thr phosphorylées (Thr177 pour la CDK6) révèle, outre une forte similarité de séquence, une différence au niveau de l’acide aminé situé en aval de la thréonine :il s’agit d’une proline dans la CDK4 et d’une sérine dans la CDK6. La mutation P173S de la CDK4 abolit la phosphorylation sur Thr172 et l’activité de la CDK4 associée à la cycline D3 dans les cellules CHO, mais n’affecte pas la phosphorylation et l’activation de la CDK4 par la CAK recombinante in vitro. L’ensemble de ces résultats suggère que la/les CAKs régulée(s) responsables de l’activation de la CDK4 n’ont pas encore été identifiées et que la proline située en aval de la Thr172 de la CDK4 est essentielle pour sa phosphorylation activatrice et son activité pRb kinase.

Doctorat en sciences biomédicales
info:eu-repo/semantics/nonPublished

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Chaves, Ferreira Miguel. „The role of cyclin D1 in lymphopoiesis“. Phd thesis, Université René Descartes - Paris V, 2012. http://tel.archives-ouvertes.fr/tel-00765134.

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D Cyclins play an essential role connecting exogenous stimulation to the intrinsic cell cycle machinery. This family of proteins is composed of three members sharing a highly homologous domain, the cyclin box (coded by exons 1-3), which is responsible for their redundant role in the phosphorylation of the retinoblastoma protein upon association with cydin-dependent kinases Cdk4/6. Both mature T and B-cells have a remarkable division capability after antigen stimulation, essential to the generation of efficient immune responses, raising the interest of D Cyclins in lymphopoiesis. Cyclin Dl, although weakly expressed by lymphocytes, is the D Cyclin most commonly implicated in lymphoid cancers and as having a Cdk-independent transcriptional role. To study the role of Cyclin Dl, we used mice deficient for the Dl cyclin box but sparing exons 4-5. Surprisingly, individual mice have very different phenotypes that we subdivided into four arbitrary groups. Group I mice show the most precocious block in lymphoid lineage differentiation, illustrated by a low cellularity of common lymphoid progenitor cells (CLP). The thymi showed very few CD4*CD8*, double positive (DP) cells, while the CD4 CD8TCR, triple negative (TN) populations were found to be mostly constituted by the early CD44*CD25' (TNI) and few CD44*CD25* (TN2). TNl's early thymocyte progenitors (ETP) were virtually absent. At the B-cell lineage level in the bone marrow (BM) there was a major block in pre-proB differentiation. The number of peripheral T-cells was severely reduced, mainly in LN, since group I T-cells lack CCR7 expression. Group II mice presented moderate thymus atrophy. The block on TN differentiation occurs at a later stage, i.e., in the TN3 to TN4 transition, and the TNI population was characterized by a less severe depletion of the ETP. Group II mice showed a partial pre-proB block and a reduction in pre-B-cells. CLPs were also reduced but to a lesser extent than in group I mice. Group ill and group IV mice appear to have a normal thymocyte population distribution but showed an increase on ETP compartment. Group IV mice displayed thymic hyperplasia while group III mice possessed normal thymus cellularity. B-cell differentiation on both groups appeared to be normal but BM precursors had an increase in both CLP and early haematopoietic progenitor's (LSK) levels as compared with wild type mice. Cyclin Dl involvement in G1 to S transition led us to analyse in vivo division rates. Strikingly, group I mice were virtually devoid of cycling cellsin all lymphoid compartments, explaining why lymphoid lineage cells do not differentiate in these mice. In contrast, in all other groups we observed an increased BrdU incorporation. These contradicting phenotypes correlated with the expression or absence of a truncated Dl protein coded by exons 4-5. The presence of the cyclin Dl truncated mRNA was not found in group I mice but high levels of expression are consistently observed in the remaining groups. In the absence of the Dl truncated protein only trace values of Cyclins D2 and D3 were found, highlighting the role of this protein as a master D cyclin regulator, which further supports the profound aplasia and arrest in lymphoid lineage division on cells that predominantly express Cyclin D2. These results suggest that, while the function of the Dl cyclin box is redundant, the regulatory domain coded by exons 4-5 is fundamental for lymphopoiesis. Full Dl protein was also eliminated by RNA interference both in vitro and in vivo. These experiments reproduced the phenotype of group I mice. We have developed a lentiviral vector with a truncated Dl (exons 4-5) and conditional knockout (KO) mice by floxing exons 4-5 of cyclin Dl. These tools will allow us to show Cyclin Dl Cdk-independent role as a transcription regulator in lymphopoiesis and to attribute this function to exons 4-5. Understanding how exons 4-5 regulate different transcription factors might be a key in...

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Sorrell, David Andrew. „CycD cyclins and cyclin-dependent kinases in tobacco BY-2 cells“. Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624421.

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Bonnet, Christine. „Un motif sur la cycline B nécessaire à l'activation de CDK1 chez la levure ?“ Paris 6, 2002. http://www.theses.fr/2002PA066509.

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Dann, Jeremiah J. „Immunological characterization and histone kinase activity of cyclin B1 and Cdk1 at G1 and G2/M phase of the cell division cycle in one-cell mouse embryos“. Virtual Press, 2004. http://liblink.bsu.edu/uhtbin/catkey/1306852.

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Cyclin B1 is a cell cycle protein typically associated with the regulation of cellular division (mitosis). Previous studies in this laboratory involving preimplantation mouse embryos found that cyclin B1, or a cyclin B 1-related protein, were present at both G1 and G2/M phase of the cell cycle. Not only was cyclin Bi detected during G1 phase in this study, it was found to be present in higher concentrations at G1 phase through the first three cell cycles. These findings were unexpected, because most of the literature suggests that cyclin B1 is normally degraded during G1 phase. Using immunoprecipitation and immunoblot techniques, a more detailed study of cyclin B1 expression was inititated. Using two different primary antibodies direct against cyclin B1, a 48.97 kDa protein band, which is believed to be cyclin B1, was detected at both G1 and G2/M phases in 1-cell mouse embryos. Using another antibody directed against Cdk1, the kinase that forms a complex with cyclin B1 in order to direct the G2/M transition, a 37 kDa protein band was also detected at both G1 and G2/M phases in 1-cell mouse embryos. In order to determine whether cyclin B1 was present as a complex with Cdk1, immunoblotting with the anti-Cdk1 antibody. Again, a 37kDa protein band was detected at both G1 and G2/M phases. Finally, in order to determine whether the cyclin B1/Cdk1 complex exists in its active form, histone kinase assays were performed using anti-cyclin B1 immunoprecipitates. Kinase activity was detected in immunoprecipitates collected from G2/M phase 1-cell embryos, but no kinase activity was detected from immunoprecipitates collected from G1 phase 1-cell embryos. These data indicate that cyclin B1 and Cdk1 are present and exist as a complex in both G1 and G2/M phases of 1-cell mouse embryos, although the complex only appears to be active at the G2/M phase.
Department of Biology

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Mata, Xavier. „Analyse structurale et fonctionnelle de gènes voisins du "locus" de l'α-lactalbumine caprine : application à la recherche d'éléments "cis"-régulateurs à effet dominant“. Limoges, 2003. http://www.theses.fr/2003LIMO0033.

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L'utilisation récente de grands fragments d'ADN (BACs et YACs) a permis de s'affranchir de " l' effet de position "en transgénèse. Cela a été le cas pour un BAC caprin de 160 kb contenant le gène de l'a-lactalbumine (BAC 41), suggérant la présence d'éléments cis-régulateurs dominants. Mon sujet de thèse visait à analyser plus finement cet insert. Des expériences de transgénèse utilisant un BAC raccourci dérivé (BAC 6) nous a permis d'effectuer une primo-localisation de ces éléments. Dans cette région deux loci ont été identifiés : celui de la cycline T1 et FLJ20436. Leur caractérisation fonctionnelle a permis de montrer qu'ils sont actifs au sein du BAC et à expression ubiquiste. De façon inattendue, l'utilisation du promoteur de la cycline T1 en transgénèse a conduit à unessur-expression dans la lignée germinale mâle. Le gène FLJ20436 présente un épissage complexe. Ces études nous ont amenés à suspecter la présence de deux domaines chromatiniens putatifs séparant ces gènes à expression ubiquiste de celui de l'a-lactalbumine. L'analyse structurale de ces loci a permis de dresser une carte précise du BAC 41 et de délimiter une région frontière séparant les deux domaines chromatiniens putatifs. Une recherche en son sein d'éléments cis-régulateurs dominants a été initiée. L'identification et l'association de tels éléments au promoteur du gène de l'a-lactalbumine devraient contribuer à la mise au point de vecteurs d'expression efficients pour la transgénèse mammaire
The recent use of large genomic fragment (BACs or YACs) has allowed to avoid this "position effect". This has been observed with a vector that was developed in our laboratory that consists of a 160 kb goat BAC insert (BAC 41) encompassing the a-lactalbumin gene, suggesting the occurrence of dominant cis-regulatory elements. The aim of this thesis was to further analyse this insert. Transgenic experiments using a derived shorter BAC of 60 kb allowed us to localise these regulatory elements in a 5' distal region of the a-lactalbumin locus. In this region two loci were identified: the cyclin T1 and FLJ20436. Characterisation of these genes revealed that they were functional within the BAC 41 and ubiquitously expressed. Surprisingly, the use of the cyclin T1 promoter in transgenics resulted in an ubiquitous expression unexpectedly high only in male germ cells. FLJ20436 pre-mRNA has a very complex splicing pattern that is conserved during evolution. These observations led us to suspect the occurrence of two chromatin domains separating these ubiquitously expressed genes from the a-lactalbumin one. Structural analysis of these genes has allowed to define a precise restriction map of the BAC 41 and to precise the location of the potential border region within the two chromatin domains. Search for cis-regulatory elements within this region was initiated. There identification and association with the a-lactalbumin promoter should contribute to the creation of efficient mammary specific expression vectors

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Martinsson, Hanna-Stina. „Single cell analysis of checkpoints in G₁ /“. Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-455-4/.

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Ji, Jun-Yuan. „Functions of Cdk1-cyclin B in regulating the early embryonic mitoses in Drosophila /“. Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/5124.

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Pontheaux, Florian. „Activité traductionnelle et dynamique mitotique induites par la fécondation chez l’oursin“. Electronic Thesis or Diss., Sorbonne université, 2022. http://www.theses.fr/2022SORUS209.

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La régulation fine de la traduction pour la dynamique du cycle cellulaire est un sujet important dans la recherche cellulaire. Au cours de ma thèse, j'ai analysé les relations entre l’activité traductionnelle des ARNm et les divisions embryonnaires mitotiques d'oursins. La fécondation de l'œuf déclenche l'activation de la machinerie traductionnelle nécessaire à la reprise des divisions mitotiques. Un réseau de régulation traductionnelle (TlRN), indépendant de la transcription, reste à identifier et à caractériser en amont des acteurs du cycle cellulaire. A la recherche d'activités mitotiques pour visualiser la dynamique spatiale à l'intérieur d'œufs, j'ai obtenu des données originales montrant l'activité dynamique et spatiale du complexe mitotique CyclinB/CDK1 et la phosphorylation de l'histone H3 sur la thréonine 3 (pH3T3) pendant la mitose embryonnaire. Ensuite, j'ai analysé le rôle in vivo de 5'UTR spécifiques pour contrôler le recrutement d'ARNm dans les polysomes actifs après la fécondation. Enfin, j'ai montré que la traduction de l'ARNm codant pour eIF4B (facteur d'initiation eucaryote 4B) contrôle l'activité traductionnelle et la dynamique des deux premières divisions mitotiques induites par la fécondation. Je propose qu'eIF4B agisse comme un régulateur positif au sein du TlRN. Ces données permettront d'étudier l'effet potentiel d'eIF4B sur les activités CDK1 et pH3T3
Fine tuning of translation for cell cycle dynamics remains an important topic in cell research. During my thesis, I analyzed the relationships between mRNA translational activity and mitotic cell division using sea urchin embryos. Egg fertilization triggers the activation of the translational machinery, which is required for resuming the first mitotic division, independently of any transcription. A Translational Regulatory Network (TlRN) remains to be identified and characterized upstream of the cell cycle actors. Seeking mitotic activities that can help visualize spatial dynamics inside isolated eggs, I obtained original data showing the spatial and dynamic activity of the mitotic complex CyclinB/CDK1 and the phosphorylation of histone H3 at threonine 3 (pH3T3) during embryonic mitosis. Then, I analyzed the in vivo role of specific 5’UTR for controlling the mRNA recruitment onto active polysome following fertilization. Finally, I showed that the translation of the mRNA encoding for eIF4B (eukaryotic Initiation Factor 4B) controls the translational activity and dynamics of the first two mitotic divisions induced by fertilization. I propose that eIF4B acts as a positive regulator within the TlRN. These data will allow to study the potential effect of eIF4B acting upstream the spatial dynamics of CDK1 and pH3T3 activities

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Bücher zum Thema "CyclinB"

Delibes, Miguel. Mi querida bicicleta. Valladolid, Spain: Miñón, 1988.

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Cycling. Oxford: Heinemann Library, 2010.

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Garrett, Greg. Cycling. New York: Kensington Books, 2003.

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Mason, Paul. Cycling. London: Franklin Watts, 2011.

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North, David. Cycling. London: Flame Tree, 2011.

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Bow, James. Cycling. London: Franklin Watts, 2013.

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W, Darst Paul, Hrsg. Cycling. Glenview, Ill: Scott, Foresman, 1987.

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Bailey, Donna. Cycling. Austin, Tex: Steck-Vaughn Library, 1991.

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Paul, Mason. Cycling. Mankato, Minn: Sea-to-Sea Publications, 2010.

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Bow, James. Cycling. London: Franklin Watts, 2009.

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Buchteile zum Thema "CyclinB"

Blethrow, Justin D., Amanda L. DiGuilio und Joseph S. Glavy. „Purification of Cdk-CyclinB-Kinase–Targeted Phosphopeptides from Nuclear Envelope“. In Methods in Molecular Biology, 271–82. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2337-4_18.

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Malumbres, Marcos. „Cyclins and Cyclin-dependent Kinases“. In Encyclopedia of Systems Biology, 509–12. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_10.

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Bundság, Éva Szabina, und Sándor Huszár. „Investigation of sense of community among cyclists“. In Green and Digital Transitions, 262–69. Szeged, Hungary: Szegedi Tudományegyetem, 2024. http://dx.doi.org/10.14232/gtk.gdtgiss.2024.15.

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Cycling has become determinate in many areas of life. It is a cheap, environmentally friendly and flexible mode of transport, and due to the emergence and development of cycle sport and the growing willingness to follow a healthy lifestyle, cycling provides sports opportunities and the daily exercise for many. The aim of this study[1] is to investigate the sense of community among cyclists by conducting an online survey using validated questions from the Sense of Community Index 2 (SCI-2) model, with 362 responses gathered. According to the results, the interpreted scales were applicable and most of the respondents agreed with the 4 dimensions of the SCI-2 (Membership, Influence, Integration and fulfilment of needs, Shared emotional connection) which assumes that cyclist do feel a kind of community with each other. From a marketing point of view, cycling can be perceived not only as an activity but also as a group of consumers linked to this activity.

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Cox, Peter. „Introduction“. In Cycling, 1–10. Abingdon, Oxon ; New York, NY : Routledge, 2019.: Routledge, 2019. http://dx.doi.org/10.4324/9781315533698-1.

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Cox, Peter. „Riding the city“. In Cycling, 174–94. Abingdon, Oxon ; New York, NY : Routledge, 2019.: Routledge, 2019. http://dx.doi.org/10.4324/9781315533698-10.

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Cox, Peter. „Towards a sociology of cycles and cycling“. In Cycling, 11–35. Abingdon, Oxon ; New York, NY : Routledge, 2019.: Routledge, 2019. http://dx.doi.org/10.4324/9781315533698-2.

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Cox, Peter. „Researching cycling“. In Cycling, 36–58. Abingdon, Oxon ; New York, NY : Routledge, 2019.: Routledge, 2019. http://dx.doi.org/10.4324/9781315533698-3.

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Cox, Peter. „Materials“. In Cycling, 59–80. Abingdon, Oxon ; New York, NY : Routledge, 2019.: Routledge, 2019. http://dx.doi.org/10.4324/9781315533698-4.

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Cox, Peter. „Environments“. In Cycling, 81–99. Abingdon, Oxon ; New York, NY : Routledge, 2019.: Routledge, 2019. http://dx.doi.org/10.4324/9781315533698-5.

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Cox, Peter. „Exploring (im)material space“. In Cycling, 100–119. Abingdon, Oxon ; New York, NY : Routledge, 2019.: Routledge, 2019. http://dx.doi.org/10.4324/9781315533698-6.

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Konferenzberichte zum Thema "CyclinB"

Gaster, Kristina, und Tina Gebiert. „Mileage-based accident risks of pedelec riders“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.416.

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Francke, Angela, Paul Papendieck, Lisa-Marie Schaefer und Juliane Anke. „Perceived cycling safety during Corona times - Results of a longitudinal study in Germany“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.446.

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Oyoo, Robert O., und S. K. Mwea. „Challenges and Opportunities in Cycling Safety in Nairobi City, Kenya“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.451.

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Tumakova, Yana, Constant Cap, Azeb T. Legese, Marie Klosterkamp und Angela Francke. „“The missing lights of Nairobi” - Cyclists' Perceptions of safety by cycling after-dark in Nairobi, Kenya“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.448.

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Shah, Nitesh R., und Christopher R. Cherry. „Riding an e-scooter at nighttime is more dangerous than at daytime“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.436.

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Ramírez-Leuro, Laura D., und Lenin A. Bulla-Cruz. „Cyclists' Safety and Security Multiple Correspondence Analysis from GPS Records for Route Choice in Bogotá - Colombia“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.447.

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Rubie, Elisabeth, Narelle Haworth und Naohide Yamamoto. „Passing distance, speed and perceived risks to the cyclist and driver in passing events“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.439.

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Legese, Azeb T., Abhimanyu Prakash, Angela Francke, Yana Tumakova, Marie Klosterkamp und Paul Papendieck. „More than a billion motives to focus on NMT Africa - Enhancing the quality of infrastructure to improve cycling safety and cycling culture in Africa, case in Ethiopia“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.450.

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Hagemeister, Carmen, und Leander Bertram. „Frequency and Legitimacy of Aggressive Driver Behaviour against Cyclists when Sharing the Road“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.441.

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Robert, Denis L. „Decreasing Automobile Collisions with Cyclists in the United States by lncreasing Automobile Driver Awareness“. In International Cycling Safety Conference. Technische Universität Dresden, 2022. http://dx.doi.org/10.25368/2022.440.

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Berichte der Organisationen zum Thema "CyclinB"

Lißner, Sven, Stefan Huber und Maike von Harten. Influence of the cycling campaign CITY CYCLING on cycling behaviour in Germany. TU Dresden, Fakultät Verkehrswissenschaften 'Friedrich List', 2023. http://dx.doi.org/10.26128/2023.65.

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The CITY CYCLING (STADTRADELN) campaign has been running since 2008 to motivate German citizens to use the bicycle for daily mobility routines. In the course of the MOVEBIS research project, nationwide GPS data of the CITY CYCLING participants were collected in the years 2018-2020 and were processed for planning purposes. This contribution addresses the question to which extent the participants in the CITY CYCLING campaign represent cyclists in the Federal Republic of Germany and whether the motivation during the campaign leads to a significant change in mobility behaviour. For this purpose, more than 73,000 complete questionnaires of campaign participants from a survey in the year 2020 were evaluated. The age and gender distribution of app users and non-users of the campaign are corresponding to those of cyclists from representative household surveys in Germany (MiD 2017). App users and non app users differ only insignificantly from each other and are, on average, rather older than in the cycling participants of nationwide MiD survey. The results reveal that the smartphone has no significant influence on the cycling behaviour of the users. The survey participants are regular cyclists. Around 88% of the respondents use the bicycle most frequently in everyday life, followed by the private car (national average) and public transport (in large cities). The influence of the campaign on the level of utilisation or the number of kilometres travelled by bike can be described as rather low, overall. Whereas 65% of the participants stated that they cycled to work just as often as outside the campaign period, 19% of the respondents used the bicycle less often for commuting and 16% more often. The results indicate that the CITY CYCLING campaign captures and represents the everyday transport behaviour of participants. During the COVID-19 pandemic, participants used their bicycles significantly more often (73%). The perception of safety is consistently high. The campaign was rated very positively and the majority of users (91%) would participate again or rather recommend the campaign to others (78%).

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JI, June HyeonJeong. Cycling with fireflies. Ames: Iowa State University, Digital Repository, November 2015. http://dx.doi.org/10.31274/itaa_proceedings-180814-1282.

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Peter A. Pryfogle. Nutrient Cycling Study. Office of Scientific and Technical Information (OSTI), September 2005. http://dx.doi.org/10.2172/966178.

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Wang, Chih-Hao, und Na Chen. Do Multi-Use-Path Accessibility and Clustering Effect Play a Role in Residents' Choice of Walking and Cycling? Mineta Transportation Institute, Juni 2021. http://dx.doi.org/10.31979/mti.2021.2011.

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The transportation studies literature recognizes the relationship between accessibility and active travel. However, there is limited research on the specific impact of walking and cycling accessibility to multi-use paths on active travel behavior. Combined with the culture of automobile dependency in the US, this knowledge gap has been making it difficult for policy-makers to encourage walking and cycling mode choices, highlighting the need to promote a walking and cycling culture in cities. In this case, a clustering effect (“you bike, I bike”) can be used as leverage to initiate such a trend. This project contributes to the literature as one of the few published research projects that considers all typical categories of explanatory variables (individual and household socioeconomics, local built environment features, and travel and residential choice attitudes) as well as two new variables (accessibility to multi-use paths calculated by ArcGIS and a clustering effect represented by spatial autocorrelation) at two levels (level 1: binary choice of cycling/waking; level 2: cycling/walking time if yes at level 1) to better understand active travel demand. We use data from the 2012 Utah Travel Survey. At the first level, we use a spatial probit model to identify whether and why Salt Lake City residents walked or cycled. The second level is the development of a spatial autoregressive model for walkers and cyclists to examine what factors affect their travel time when using walking or cycling modes. The results from both levels, obtained while controlling for individual, attitudinal, and built-environment variables, show that accessibility to multi-use paths and a clustering effect (spatial autocorrelation) influence active travel behavior in different ways. Specifically, a cyclist is likely to cycle more when seeing more cyclists around. These findings provide analytical evidence to decision-makers for efficiently evaluating and deciding between plans and policies to enhance active transportation based on the two modeling approaches to assessing travel behavior described above.

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Peggs, Stephen, und Michael Furey. Rapid Cycling Medical Synchrotron. Office of Scientific and Technical Information (OSTI), Juli 2007. http://dx.doi.org/10.2172/973828.

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Kumar, Nikil, Philip Besuner, Steven Lefton, Dwight Agan und Douglas Hilleman. Power Plant Cycling Costs. Office of Scientific and Technical Information (OSTI), Juli 2012. http://dx.doi.org/10.2172/1046269.

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Vyvial, Brent. PR201-174510-R01 Removal and Repair of Hot Taps. Chantilly, Virginia: Pipeline Research Council International, Inc. (PRCI), Februar 2019. http://dx.doi.org/10.55274/r0011557.

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This project evaluated the performance of various configurations of welded repairs for use in reinforcing improper hot taps. A combination of finite element analysis and full-scale testing was used to evaluate the different repairs. The repairs were compared based primarily on fatigue performance. Samples were created using field weld procedures, and full scale testing of these samples was conducted in which stresses were measured at a number of locations using strain gages. Cyclic pressure testing of the samples at a stress range intended to represent 20 years of "moderate cycling" was used to demonstrate the fatigue performance of the repairs.

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Hogan, Eileen, und Becci Jeffers. A sense of freedom: Exploring everyday experiences of cycling in an Irish regional city. Institute for Social Science in the 21st Century, University College Cork, 2023. http://dx.doi.org/10.33178/10468/15138.

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In current policy and planning discourse, there is great interest in reimagining and redesigning cities as more cycling-friendly spaces. Research shows that good cycling infrastructure is the first step to increasing cycling rates. However, social and cultural factors are also important for understanding the attractions of cycling and barriers to cycling but these tend to be under-explored in transport policy and research. In partnership with the Cork Environmental Forum and the Cork Cycling Campaign, this study set out to develop social scientific knowledge about cycling as a social practice, focusing on Cork as a case study of an Irish regional city, with a view to informing pro-cycling policy.

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Rohrer, Lisa, Johannes Lidmo und Christoph Beidenhauser. Nordic cycling policy: National objectives, mechanisms, and actors in Denmark, Finland, Norway, and Sweden. Nordregio, November 2024. http://dx.doi.org/10.6027/wp2023:81403-2511.

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This paper reviews how Nordic countries are working to improving cycling via policy and planning. It takes a national-level approach to review cycling objectives in Denmark, Finland, Norway, and Sweden, reviews a range of mechanisms to achieve these objectives, and identifies the key actors responsible for carrying out the work. In the discussion section, the paper identifies several findings from the review work with regards to how the Nordic countries are currently addressing cycling at the policy level: - Cycling is primarily discussed as a means for contributing to environmental goals, such as GHG emission reductions, but is occasionally discussed as a means for improving health and well-being. Much less policy discourse focuses on economic or other social benefits of cycling. The primary method for reducing GHG emissions in the transportation sector focuses on replacing fossil fuel cars with electric-powered vehicles rather than on cycling infrastructure or spatial forms that decrease overall mobility requirements. - The Nordic countries have some distinctions with regards to how spatial planning is operationalised, which influences how cycling is prioritised and managed. - Beyond a dedicated cycling strategy, cycling objectives are often baked into other key plans and documents at the national level. - Achieving national cycling objectives requires clear communication among local, regional, and national actors and across ministries, agencies, and departments, which is often a challenge. - Political turnover can be a hindrance to gaining support for long-term cycling projects. - There are many different indicators and ways to measure success for cycling objectives in the Nordic countries. The paper also highlights some of the developments taking place at the UN and EU levels, making cycling and its potential for improving cities and regions more visible across international policy.

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Krantz, F. M., und M. W. Richter. Mission/Maintenance/Cycling Effects on Reliability. Fort Belvoir, VA: Defense Technical Information Center, Dezember 1991. http://dx.doi.org/10.21236/ada251921.

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