Zeitschriftenartikel: „Construction kits“

1

Resnick, Mitchel. „Behavior construction kits“. Communications of the ACM 36, Nr. 7 (Juli 1993): 64–71. http://dx.doi.org/10.1145/159544.159593.

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2

Fischer, Gerhard, und Andreas C. Lemke. „Construction Kits and Design Environments“. ACM SIGCHI Bulletin 20, Nr. 1 (Juli 1988): 81. http://dx.doi.org/10.1145/49103.1046496.

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3

HAVELKA, Martin, und Pavlína ČÁSTKOVÁ. „USING THE LEGO CONSTRUCTION KITS IN PRESCHOOL EDUCATION“. Trends in Education 8, Nr. 1 (01.07.2015): 102–12. http://dx.doi.org/10.5507/tvv.2015.004.

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4

Permyakov, V. B., R. F. Salikhov, G. N. Musagitova und N. Yu Levin. „Designing optimal structure of road construction machines kits“. Journal of Physics: Conference Series 1260 (August 2019): 082004. http://dx.doi.org/10.1088/1742-6596/1260/8/082004.

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5

SERAFÍN, Čestmír. „ELECTRICAL CONSTRUCTION KITS AND THEIR INFLUENCE ON TEACHING“. Journal of Technology and Information 4, Nr. 1 (01.04.2012): 46–49. http://dx.doi.org/10.5507/jtie.2012.008.

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6

MINARČÍK, Josef, und Martin HAVELKA. „THE CONSTRUCTION KITS II IN GENERAL TECHNICAL EDUCATION“. Journal of Technology and Information 4, Nr. 3 (01.12.2012): 95–98. http://dx.doi.org/10.5507/jtie.2012.060.

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7

Moon, Byung-Chul, Dong-Soo Kim und Hee-Wan Kim. „The Audit Method for Efficient Hospital Information System Construction“. Journal of the Korea society of IT services 11, Nr. 2 (30.06.2012): 197–211. http://dx.doi.org/10.9716/kits.2012.11.2.197.

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8

Dittert, Nadine, Eva-Sophie Katterfeldt und Heidi Schelhowe. „Die EduWear-Umgebung – Wearables konstruierend be-greifen“. i-com 11, Nr. 2 (August 2012): 37–43. http://dx.doi.org/10.1524/icom.2012.0024.

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ZusammenfassungDieser Artikel stellt das EduWear Kit vor – ein so genanntes Construction Kit mit Smart Textiles, bestehend aus Hardware, Programmierumgebung, Workshopkonzept, Webplattform und Anleitungen, mit denen eigene “Wearables” konstruiert werden können. Durch die Eigenschaften von Smart Textiles spricht das Kit auch weniger technik-affine Menschen an. Wir erläutern das Konzept der Construction Kits, stellen die Komponenten des EduWear Kits vor und geben Beispiele, wie das Kit zum be-greifen von Programmierbarkeit und Wearable Computing Konzepten vielfältig eingesetzt werden kann.

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D’Amico, Antonella, und Domenico Guastella. „The Robotic Construction Kit as a Tool for Cognitive Stimulation in Children and Adolescents: The RE4BES Protocol“. Robotics 8, Nr. 1 (30.01.2019): 8. http://dx.doi.org/10.3390/robotics8010008.

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Through numerous experiences, the robotics has been demonstrated to have good potential in the field of strengthening social skills in children with Special Educational Needs and in particular with autism spectrum disorder. There are still not many experimental studies on the cognitive enhancement and social skills of children with special needs conducted with robotics construction kits that, requiring both the construction of the robot body and the programming of its “mind“, bring into play a multiplicity of cognitive and social skills. For the aforementioned reasons our team from the University of Palermo and from the Center MetaIntelligenze ONLUS developed the treatment protocol RE4BES, which is a collection of guidelines for realizing robotics personalized activities for children with special needs. In this paper, two studies will be described concerning the first application of activities drawn from the RE4BES protocol. The first study concerns the use of the robotic construction kits for the stimulation of visuo-spatial abilities; in the second study the robot construction kits have been used to stimulate the attentional abilities in a child with severe difficulties on focused attention tasks.

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Kwon, Hyeog-In, Yun-Bin Na und Jong-Suk Park. „Platform Based of The Major Attribute Research for The Service Ecosystem Construction“. Journal of the Korea society of IT services 12, Nr. 4 (31.12.2013): 461–72. http://dx.doi.org/10.9716/kits.2013.12.4.461.

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11

Yang, Chang-Mo, Seung-Beom Lee, Soo-Hwan Choi und Doo-Seop Eom. „Empirical Evaluation of Wireless Mesh Networks for Construction IT in Tunnel Environments“. Journal of the Korea society of IT services 10, Nr. 2 (30.06.2011): 271–79. http://dx.doi.org/10.9716/kits.2011.10.2.271.

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12

Lee, Kyoung-Jun, und Jung-Ho Jun. „SPB(Seamlessness-Privacy-Benefit) Paradigm for Construction of Highly-Connected Information Society“. Journal of the Korea society of IT services 11, Nr. 2 (30.06.2012): 131–46. http://dx.doi.org/10.9716/kits.2012.11.2.131.

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13

Steele, John H., Kerim Aydin, Dian J. Gifford und Eileen E. Hofmann. „Construction kits or virtual worlds; Management applications of E2E models“. Journal of Marine Systems 109-110 (Januar 2013): 103–8. http://dx.doi.org/10.1016/j.jmarsys.2011.10.016.

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14

Guerrero-Rivera, Ruben, Abigail Morrison, Markus Diesmann und Tim C. Pearce. „Programmable Logic Construction Kits for Hyper-Real-Time Neuronal Modeling“. Neural Computation 18, Nr. 11 (November 2006): 2651–79. http://dx.doi.org/10.1162/neco.2006.18.11.2651.

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Programmable logic designs are presented that achieve exact integration of leaky integrate-and-fire soma and dynamical synapse neuronal models and incorporate spike-time dependent plasticity and axonal delays. Highly accurate numerical performance has been achieved by modifying simpler forward-Euler-based circuitry requiring minimal circuit allocation, which, as we show, behaves equivalently to exact integration. These designs have been implemented and simulated at the behavioral and physical device levels, demonstrating close agreement with both numerical and analytical results. By exploiting finely grained parallelism and single clock cycle numerical iteration, these designs achieve simulation speeds at least five orders of magnitude faster than the nervous system, termed here hyper-real-time operation, when deployed on commercially available field-programmable gate array (FPGA) devices. Taken together, our designs form a programmable logic construction kit of commonly used neuronal model elements that supports the building of large and complex architectures of spiking neuron networks for real-time neuromorphic implementation, neurophysiological interfacing, or efficient parameter space investigations.

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Ki, Yong-Kul, Gye-Hyeong Ahn, Eun-Jeong Kim und Kwang-Soo Bae. „A Study on the Construction of Historical Profiles for Travel Speed Prediction Using UTIS“. Journal of The Korea Institute of Intelligent Transport Systems 11, Nr. 6 (30.12.2012): 40–48. http://dx.doi.org/10.12815/kits.2012.11.6.040.

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Ki, Yong-Kul, Gye-Hyeong Ahn, Eun-Jeong Kim und Kwang-Soo Bae. „A Study on the Construction of Historical Profiles for Travel Speed Prediction Using UTIS“. Journal of The Korea Institute of Intelligent Transport Systems 11, Nr. 6 (30.12.2012): 40–48. http://dx.doi.org/10.12815/kits.2012.11.6.40.

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17

Lee, Heon-Yeong, Yeseul Choi und Up Lim. „Regional Economic Impacts Induced by u-City Construction in Wha-sung and Dong-tan City“. Journal of the Korea society of IT services 11, Nr. 4 (31.12.2012): 25–37. http://dx.doi.org/10.9716/kits.2012.11.4.025.

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18

Carlisle, Ben, und Paul Webb. „Construction kits: a platform for improving how civil engineers deliver infrastructure“. Proceedings of the Institution of Civil Engineers - Civil Engineering 174, Nr. 1 (Februar 2021): 10. http://dx.doi.org/10.1680/jcien.2021.174.1.10.

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19

Dybina, O. V. „MODELING OF OBJECT-SPATIAL ENVIRONMENT BY MEANS OF MODULAR CONSTRUCTION KITS“. Vektor nauki Tol'yattinskogo gosudarstvennogo universiteta. Seriya Pedagogika i psihologiya, Nr. 1 (2017): 31–37. http://dx.doi.org/10.18323/2221-5662-2017-1-31-37.

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20

Fischer, Gerhard, und Andreas C. Lemke. „Construction Kits and Design Environments: Steps Toward Human Problem-Domain Communication“. Human–Computer Interaction 3, Nr. 3 (September 1987): 179–222. http://dx.doi.org/10.1207/s15327051hci0303_1.

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21

Somyürek, Sibel. „An effective educational tool: construction kits for fun and meaningful learning“. International Journal of Technology and Design Education 25, Nr. 1 (20.04.2014): 25–41. http://dx.doi.org/10.1007/s10798-014-9272-1.

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22

Sullivan, Florence R., und John Heffernan. „Robotic Construction Kits as Computational Manipulatives for Learning in the STEM Disciplines“. Journal of Research on Technology in Education 48, Nr. 2 (29.02.2016): 105–28. http://dx.doi.org/10.1080/15391523.2016.1146563.

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23

Vandevelde, Cesar, Francis Wyffels, Maria-Cristina Ciocci, Bram Vanderborght und Jelle Saldien. „Design and evaluation of a DIY construction system for educational robot kits“. International Journal of Technology and Design Education 26, Nr. 4 (18.07.2015): 521–40. http://dx.doi.org/10.1007/s10798-015-9324-1.

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24

Park, Yong-Soo, Songmi Kim, Dong-Guk Park, Dong Hee Kim, Kyeong-Wook Yoon, Wonseok Shin und Kyudong Han. „Comparison of library construction kits for mRNA sequencing in the Illumina platform“. Genes & Genomics 41, Nr. 10 (26.07.2019): 1233–40. http://dx.doi.org/10.1007/s13258-019-00853-3.

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25

Kim, Jong Woo, und Young Woo Jung. „Analysis of the Driving & Loading Pattern of the Construction Waste Collecting Trucks Using IoT On-Board Truck Scale System“. Journal of The Korea Institute of Intelligent Transport Systems 19, Nr. 6 (31.12.2020): 74–87. http://dx.doi.org/10.12815/kits.2020.19.6.74.

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26

Kim, Jong Woo, und Young Woo Jung. „Analysis of the Driving & Loading Pattern of the Construction Waste Collecting Trucks Using IoT On-Board Truck Scale System“. Journal of The Korea Institute of Intelligent Transport Systems 19, Nr. 6 (31.12.2020): 74–87. http://dx.doi.org/10.12815/kits.2020.19.6.74.

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27

Angthong, Pacharaporn, Tanaporn Uengwetwanit, Wirulda Pootakham, Kanchana Sittikankaew, Chutima Sonthirod, Duangjai Sangsrakru, Thippawan Yoocha et al. „Optimization of high molecular weight DNA extraction methods in shrimp for a long-read sequencing platform“. PeerJ 8 (13.11.2020): e10340. http://dx.doi.org/10.7717/peerj.10340.

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Marine organisms are important to global food security as they are the largest source of animal proteins feeding mankind. Genomics-assisted aquaculture can increase yield while preserving the environment to ensure sufficient and sustainable production for global food security. However, only few high-quality genome sequences of marine organisms, especially shellfish, are available to the public partly because of the difficulty in the sequence assembly due to the complex nature of their genomes. A key step for a successful genome sequencing is the preparation of high-quality high molecular weight (HMW) genomic DNA. This study evaluated the effectiveness of five DNA extraction protocols (CTAB, Genomic-tip, Mollusc DNA, TIANamp Marine Animals DNA, and Sbeadex livestock kits) in obtaining shrimp HMW DNA for a long-read sequencing platform. DNA samples were assessed for quality and quantity using a Qubit fluorometer, NanoDrop spectrophotometer and pulsed-field gel electrophoresis. Among the five extraction methods examined without further optimization, the Genomic-tip kit yielded genomic DNA with the highest quality. However, further modifications of these established protocols might yield even better DNA quality and quantity. To further investigate whether the obtained genomic DNA could be used in a long-read sequencing application, DNA samples from the top three extraction methods (CTAB method, Genomic-tip and Mollusc DNA kits) were used for Pacific Biosciences (PacBio) library construction and sequencing. Genomic DNA obtained from Genomic-tip and Mollusc DNA kits allowed successful library construction, while the DNA obtained from the CTAB method did not. Genomic DNA isolated using the Genomic-tip kit yielded a higher number of long reads (N50 of 14.57 Kb) than those obtained from Mollusc DNA kits (N50 of 9.74 Kb). Thus, this study identified an effective extraction method for high-quality HMW genomic DNA of shrimp that can be applied to other marine organisms for a long-read sequencing platform.

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Gonçalves, Rogério Sales. „Application of LEGO Mindstorms Kits for Teaching Mechatronics Engineering“. International Journal for Innovation Education and Research 5, Nr. 10 (31.10.2017): 99–113. http://dx.doi.org/10.31686/ijier.vol5.iss10.830.

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One of the major educators’ challenges is to teach the theoretical lessons with practical examples that can be taught in the classroom or teaching laboratories. The application of these examples will face a major problem for students in engineering: the difficulty of understanding and seeing how a mechatronic device works in everyday life. This requires the use of tools that enable the construction of different low cost prototypes to assist in student learning. Another challenge to educators is the need to motivate students during the lessons and to present models that students can make and develop on their own. Within this context this paper presents a pedagogic proposition based on the use of LEGO Mindstorms kits to teach practical lab activities in a mechatronics engineering course. The objective is to develop teaching methodologies with the use of these LEGO kits in order to motivate the students and also to promote a higher interdisciplinarity, by proposing projects that unify different disciplines. Thus, the paper is divided into three parts according to the educational experiences implemented in the course of mechatronics engineering at the Federal University of Uberlândia, Brazil. The first part presents the use of the kits in robotics discipline. The second part presents the use of the virtual kits in the Computer Aided Design discipline with zero-cost. The third part presents a multi-disciplinary project EDROM in mechatronics using LEGO kits.

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Stridsberg, M., B. Eriksson, K. Oberg und ET Janson. „A comparison between three commercial kits for chromogranin A measurements“. Journal of Endocrinology 177, Nr. 2 (01.05.2003): 337–41. http://dx.doi.org/10.1677/joe.0.1770337.

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Chromogranin (CgA) has been shown to be an excellent marker for neuroendocrine tumours. There are now three commercial assays on the market. We wanted to compare the usefulness of the different kits in a clinical situation. We have thus measured CgA in 77 patients and compared the results from the different methods. CgA was measured with three different commercial kits according to the recommendations from the manufacturers (CGA-RIA CT; CIS bio international, Gif-sur-Yvette cedex, France, DAKO Chromogranin A ELISA kit; DAKO A/S, Glostrup, Denmark and CgA; EuroDiagnostica, Malmo, Sweden). The sensitivity and specificity differed between the different kits. The CIS kit showed a sensitivity of 67% and a specificity of 96%. The sensitivity and specificity were both 85% for the DAKO kit and 93% and 88% respectively for the EuroDiagnostica assay. We have concluded that CgA is an important tumour marker for all neuroendocrine tumours. However, different analytical properties of the respective kits give different performances, a fact that must be taken into consideration when comparing results from different clinical studies. A recognised international standard for CgA would be a step on the way to harmonisation, but antibody selection and construction of the assays will probably still influence the results.

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Fomina, E. G., E. E. Grigorieva und A. S. Vladyko. „Recombinant Retroviral Particles: Technology of Poduction and Application as Positive Controls for PCR Diagnostics of Dangerous Viral Infections“. Problems of Particularly Dangerous Infections, Nr. 2 (12.07.2020): 115–21. http://dx.doi.org/10.21055/0370-1069-2020-2-115-121.

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Objective. Construction of positive control samples based on recombinant retroviral particles and their application in RT-PCR diagnostic assays for RNA detection of agents of dangerous and particularly dangerous viral infections.Materials and methods. Molecular biological, genetic engineering, and immunological methods were used: polymerase chain reaction, restriction, ligation, cloning, transformation, transfection, flow cytometry.Results and discussion. Technology of positive control samples producing based on recombinant virions has been developed and tested. It includes construction of retroviral vector with cloned diagnostic sequence of the viral genome; obtaining a packaging cell line producing chimeric retroviral particles; determination of recombinant virions titer by flow cytometry and polymerase chain reaction; application of the obtained preparation as a control sample for PCR diagnostics of infectious agents. Positive controls based on retroviral vectors as carriers of genomic RNA fragments of pathogenic viruses were used in the development of PCR diagnostic kits for dangerous and particularly dangerous viral infections. Their application increased the kits quality and made it possible to exclude the work with concentrated hazardous infectious agents (Lassa virus, tick-borne encephalitis virus, lymphocytic choriomeningitis virus, Puumala virus).

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Gross, Mark D. „Why can't CAD be more like Lego? CKB, a program for building construction kits“. Automation in Construction 5, Nr. 4 (Oktober 1996): 285–300. http://dx.doi.org/10.1016/s0926-5805(96)00154-9.

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32

WILKERSON-JERDE, MICHELLE, ADITI WAGH und URI WILENSKY. „Balancing Curricular and Pedagogical Needs in Computational Construction Kits: Lessons From the DeltaTick Project“. Science Education 99, Nr. 3 (15.04.2015): 465–99. http://dx.doi.org/10.1002/sce.21157.

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Li, Feng, Karolina Elżbieta Kaczor-Urbanowicz, Jie Sun, Blanca Majem, Hsien-Chun Lo, Yong Kim, Kikuye Koyano et al. „Characterization of Human Salivary Extracellular RNA by Next-generation Sequencing“. Clinical Chemistry 64, Nr. 7 (01.07.2018): 1085–95. http://dx.doi.org/10.1373/clinchem.2017.285072.

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Abstract BACKGROUND It was recently discovered that abundant and stable extracellular RNA (exRNA) species exist in bodily fluids. Saliva is an emerging biofluid for biomarker development for noninvasive detection and screening of local and systemic diseases. Use of RNA-Sequencing (RNA-Seq) to profile exRNA is rapidly growing; however, no single preparation and analysis protocol can be used for all biofluids. Specifically, RNA-Seq of saliva is particularly challenging owing to high abundance of bacterial contents and low abundance of salivary exRNA. Given the laborious procedures needed for RNA-Seq library construction, sequencing, data storage, and data analysis, saliva-specific and optimized protocols are essential. METHODS We compared different RNA isolation methods and library construction kits for long and small RNA sequencing. The role of ribosomal RNA (rRNA) depletion also was evaluated. RESULTS The miRNeasy Micro Kit (Qiagen) showed the highest total RNA yield (70.8 ng/mL cell-free saliva) and best small RNA recovery, and the NEBNext library preparation kits resulted in the highest number of detected human genes [5649–6813 at 1 reads per kilobase RNA per million mapped (RPKM)] and small RNAs [482–696 microRNAs (miRNAs) and 190–214 other small RNAs]. The proportion of human RNA-Seq reads was much higher in rRNA-depleted saliva samples (41%) than in samples without rRNA depletion (14%). In addition, the transfer RNA (tRNA)-derived RNA fragments (tRFs), a novel class of small RNAs, were highly abundant in human saliva, specifically tRF-4 (4%) and tRF-5 (15.25%). CONCLUSIONS Our results may help in selection of the best adapted methods of RNA isolation and small and long RNA library constructions for salivary exRNA studies.

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Sooklal, Valmiki K. „Development of a Modular Home Construction Kit using the Rammed Earth Building Technique“. International Journal for Service Learning in Engineering, Humanitarian Engineering and Social Entrepreneurship 10, Nr. 1 (25.06.2015): 24–37. http://dx.doi.org/10.24908/ijsle.v10i1.5706.

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In developing countries where resources are limited, the task of providing shelter for poverty stricken communities that are already in dire need of basic amenities can be a daunting one. This paper proposes a solution to this housing issue in the form of a modular home construction kit. These kits will provide such communities with a tool for sustainably constructing their own dwellings using local manpower combined with the natural resources available on site. The technique is based on the rammed earth principle in which the raw material for the construction process is primarily the natural soil present at the targeted location. This result in considerable savings in terms of sourcing and transporting costs of building materials typically used in traditional constructions methods. In addition, heavy or powered equipment is not needed for this construction technique making it a viable option for places where an electrical power supply or fuel source is not available.The project is part of a service-learning program, recently introduced at the University of Colorado at Colorado Springs to afford students an opportunity to work on a multidisciplinary team while using their engineering knowledge to provide solutions to real world problems. The student team working on the project has been involved in developing all the necessary procedures for assessment of the location’s soil type as well as the design and sourcing of the kit components. They will also be required to develop the necessary instructional and training material to allow for assembly of the kit components and the implementation of the technique to produce a finished structure (excluding the roof).

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Buech, Richard R. „Sex differences in behavior of beavers living in near-boreal lake habitat“. Canadian Journal of Zoology 73, Nr. 11 (01.11.1995): 2133–43. http://dx.doi.org/10.1139/z95-250.

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Comparative studies of how female and male North American beavers (Castor canadensis) allocate time provide a basis for understanding their life history. I studied the behavior of beavers living in lake habitats of a near-boreal region to determine how animals of each sex allocated time during their active periods. Markov time-budget estimates revealed that over the open-water season, adult females and males spent 91 and 86%, respectively, of time during active periods feeding, traveling, and being in the lodge. Adult females spent most of their time feeding in late spring and summer. In late summer through fall, they spent more time provisioning, working on lodges, and constructing winter food caches. In contrast, adult males spent less time feeding and more time traveling, being in the lodge, and working on the lodge in late spring and early summer. As the season progressed, adult males traveled less and spent more time feeding and working on the lodge. Overall, the results suggest that there is a division of labor in this monogamous species. Adult females seemed to function primarily as providers of energy to kits. In contrast, adult males seemed to function primarily in protection and provisioning of kits, territory maintenance, and construction and maintenance of structures.

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Bartlett, Francis G. „Interim Products and Concepts of Their Management“. Journal of Ship Production 3, Nr. 02 (01.05.1987): 119–27. http://dx.doi.org/10.5957/jsp.1987.3.2.119.

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This paper discusses the progress leading to the development of interim products and proposes their division into four different types of increasing complexity. Database records prepared for each interim product and their material kits are introduced for use in a management system to direct the movement of materials through work stations and to record all administrative data needed to ensure that the vessel will be built within estimates of labor and materials and within the contracted time. In addition, the records of materials, of material kits and of simple interim products are searched and sorted, using database programming, to find items having similar repetitive work processes. Those aforementioned items having these characteristics are collected into groups for their manufacture in specially equipped work stations of a ship's production system. The commercial shipbuilding practices forming the basis for the majority of available references have favored the paper's emphasis on merchant ship construction. Most of the ideas presented can be readily applied to naval shipbuilding.

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D’Amico, Antonella, und Domenico Guastella. „Robotics Construction Kits: From “Objects to Think with” to “Objects to Think and to Emote with”“. Future Internet 10, Nr. 2 (24.02.2018): 21. http://dx.doi.org/10.3390/fi10020021.

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Dodd, Matt, Brett Hartshorne, Werner Liebau und Rick Seaman. „Peel-Caribou Staging Area, Yukon Territory: assessment and remediation of DDT and other contaminants along a riverbank“. Polar Record 39, Nr. 4 (Oktober 2003): 347–55. http://dx.doi.org/10.1017/s003224740300295x.

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The Peel-Caribou Staging Area, located along the Peel River, northern Yukon Territory, was used for exploratory drilling activities during the mid-1960s. Following abandonment of the project in 1975, waste materials were buried in a pit on site. An encroachment of the river on the waste pit in 1994 exposed the debris along with soils contamiated by DDT (1,1,1-trichloro-2,2-bis-(4-chlorophenyl)ethane), metals, hydrocarbons, and dichloromethane. The results of subsurface drilling in April 2000 and contaminants analyses, along with data from a geophysical investigation, indicated contaminated soils and debris were located in a 2-metre thick layer below the high-water mark of the riverbank, with an overburden of soil varying in depths from 3 to 4 m. The validation of various field test kits — including immunoassay test kits, PetroFLAGTM analyzer, and photoionization detector — as possible analytical tools for on-site testing was conducted during the site investigations. Site remediation was conducted in January–March 2002 while the river was frozen to facilitate excavations along the frozen and exposed riverbank as well as site access. The debris along the riverbank is usually submerged in the summer and autumn due to fluctuating water levels. Following the construction of a winter overland route to the site, the contaminated soils and associated waste were excavated and transported off-site for disposal. Based on the validation exercise, immunoassay test kits were used for on-site delineation and confirmatory testing during site remediation.

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Regnault, Béatrice, Thomas Bigot, Laurence Ma, Philippe Pérot, Sarah Temmam und Marc Eloit. „Deep Impact of Random Amplification and Library Construction Methods on Viral Metagenomics Results“. Viruses 13, Nr. 2 (07.02.2021): 253. http://dx.doi.org/10.3390/v13020253.

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Clinical metagenomics is a broad-range agnostic detection method of pathogens, including novel microorganisms. A major limit is the low pathogen load compared to the high background of host nucleic acids. To overcome this issue, several solutions exist, such as applying a very high depth of sequencing, or performing a relative enrichment of viral genomes associated with capsids. At the end, the quantity of total nucleic acids is often below the concentrations recommended by the manufacturers of library kits, which necessitates to random amplify nucleic acids. Using a pool of 26 viruses representative of viral diversity, we observed a deep impact of the nature of sample (total nucleic acids versus RNA only), the reverse transcription, the random amplification and library construction method on virus recovery. We further optimized the two most promising methods and assessed their performance with fully characterized reference virus stocks. Good genome coverage and limit of detection lower than 100 or 1000 genome copies per mL of plasma, depending on the genome viral type, were obtained from a three million reads dataset. Our study reveals that optimized random amplification is a technique of choice when insufficient amounts of nucleic acid are available for direct libraries constructions.

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Resnick, Mitchel. „Learning About Life“. Artificial Life 1, Nr. 1_2 (Oktober 1993): 229–41. http://dx.doi.org/10.1162/artl.1993.1.1_2.229.

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The growing interest in Artificial Life is part of a broader intellectual movement toward decentralized models and metaphors. But even as decentralized ideas spread through the culture, there is a deep-seated resistance to these ideas. People have strong attachments to centralized ways of thinking: they often assume centralized control where none exists. New types of computational tools and construction kits are needed to help people move beyond this “centralized mindset.” Perhaps most important are new tools and activities for children, to help them develop new ways of looking at the world.

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Amosova, I. V., und M. P. Grudinin. „Diagnostic Potential of Monoclonal Antibodies to Adenovirus“. Biotekhnologiya 37, Nr. 2 (2021): 48–53. http://dx.doi.org/10.21519/0234-2758-2021-37-2-48-53.

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The diagnostic potential of 4B7 and 6B12 monoclonal antibodies (mAbs) against human adenovirus hexon protein has been studied in various immunological tests, namely: in-cell enzyme-linked immunosorbent assay (cell-ELISA), sandwich ELISA, and immunofluorescent assay (IFA). It was shown that the sensitivity of cell-ELISA, sandwich ELISA and IFA was 96%, 86% and 84%, respectively as compared to PCR. Thus, 4B7 and 6B12 mAbs are promising immunoreagents for the construction of various diagnostic kits to use in laboratory practice for adenovirus detection in clinical samples. monoclonal antibodies, human adenovirus, adenovirus infection, diagnosis

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Sass, Lawrence. „Parametric Constructionist Kits: Physical Design and Delivery System for Rapid Prototyping Devices“. International Journal of Architectural Computing 7, Nr. 4 (Dezember 2009): 623–42. http://dx.doi.org/10.1260/1478-0771.7.4.623.

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Fukunaga, Keisuke, und Masumi Taki. „Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems“. Journal of Nucleic Acids 2012 (2012): 1–9. http://dx.doi.org/10.1155/2012/295719.

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Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide. Commercially available premade phage libraries allow us to take screening in the easiest way. On the other hand, construction of a custom phage library seems to be inaccessible, because several practical tips are absent in instructions. This paper focuses on what should be born in mind for beginners using commercially available cloning kits (Ph.D. with type 3 vector and T7Select systems for M13 and T7 phage, respectively). In the M13 system, Pro or a basic amino acid (especially, Arg) should be avoided at the N-terminus of peptide fused to gp3. In both systems, peptides containing odd number(s) of Cys should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.

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Ellwood, Elizabeth, Kathryn Estes-Smargiassi, Noel Graham, Gary Takeuchi, Austin Hendy, Molly Porter und Emily Lindsey. „Project Paleo: Citizen Curation and Community Science at the Natural History Museum of Los Angeles County“. Biodiversity Information Science and Standards 2 (15.06.2018): e25980. http://dx.doi.org/10.3897/biss.2.25980.

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The School and Teacher Programs of the Natural History Museum of Los Angeles County have partnered with the La Brea Tar Pits and Museum (LBTPM) and the Invertebrate Paleontology (LACMIP) collection to create two “citizen curation” exercises dubbed “Project Paleo”. Classroom kits were created with unsorted fossils from either LBPTM or from a local invertebrate paleontological field site, to be sorted and identified by local elementary and middle school students and then returned to the museum for curation, analysis, and research purposes. Each kit contains background information about the project and fossils, and an identification guide to assist the students and teachers. The “Project Paleo: Rancho La Brea” kit contains three tablespoons of unsorted fossil matrix from LBTPM’s Project 23. Groups of students learn about past and present food webs of the Los Angeles Basin, then sort the matrix into several categories (bones, plants, other fossils, and rocks) using a guide with drawn examples of each. An online iNaturalist (inaturalist.org) project also serves as an identification resource as well as a platform by which students can contribute photos for identification by staff researchers. This project is aimed at middle schoolers and over 700 students have used the sorting kits. Results will help to recreate past ecosystems of Southern California and help to inform a National Science Foundation (NSF) funded project, “A Mouse’s Eye View of Rancho La Brea”. The “Project Paleo: Marine Invertebrates of Southern California” kit produced by LACMIP, contains approximately two cups of washed but unsorted coarse fossil matrix from a salvaged (now destroyed) construction site. This kit is aimed at 5th grade Los Angeles Unified School District classrooms and homeschooling families. Students are asked to sort fossils by species and use included identification cards to identify the sorted fossils to the best of their ability. Results of this project will be included in an NSF funded digitization project and will contribute to research on the paleoecology of Pleistocene Southern California. Early evaluation of both kits has shown positive feedback from students and educators, as well as some room to improve instructions to students. These kits are designed to conform to Next Generation Science Standards while generating useful data for museum scientists. Collections staff are able to outsource the curation of critical data to students who get the experience of handling real museum fossils and contributing to the body of paleontological research.

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Choi, Chang Hwan, Kyu Ho Kim, Ju Young Song, Suk Jin Choi, Lucia Kim, In Suh Park, Jee Young Han, Joon Mee Kim und Young Chae Chu. „Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks“. Korean Journal of Pathology 46, Nr. 6 (2012): 562. http://dx.doi.org/10.4132/koreanjpathol.2012.46.6.562.

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Lin, Xiaojing, Lihong Qiu, Xue Song, Junyan Hou, Weizhi Chen und Jun Zhao. „A comparative analysis of RNA sequencing methods with ribosome RNA depletion for degraded and low-input total RNA from formalin-fixed and paraffin-embedded samples.“ Journal of Clinical Oncology 37, Nr. 15_suppl (20.05.2019): e14654-e14654. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.e14654.

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e14654 Background: Formalin-fixed and paraffin-embedded (FFPE) blocks held in clinical laboratories are an invaluable resource for clinical research, especially in the era of personalized medicine. With the development of massive parallel sequencing, RNA expression profiles have become an important source of new biomarkers with potential values in cancer metastasis and disease prognosis. Standard practice in tissue fixing and paraffin-embedding has little impact on the expression analysis of RNA samples, which makes archived FFPE samples valuable and retrospective studies feasible. But it is still difficult for some FFPE samples which have low quality or low amount of RNA to be used in the RNA expression study. Methods: We compared four FFPE RNA library preparation kits (KAPA, TaKaRa, QIAGEN and Vazyme) based on two principles of rRNA depletion, with total RNA from FFPE samples and paired FF samples using two bioinformatics analysis methods. Furthermore, we tried to construct RNA libraries with the two kits allowing for degraded low-input RNA using clinical FFPE samples of different quality. HISAT was used to analyze the data of all the libraries. Results: Our results demonstrated that high concordance in transcript quantifications were shown between FF and FFPE samples using the same kit and more transcripts could be obtained by rRNA depletion after reverse transcript reaction. The gene expression profiling using the TaKaRa kit showed a difference with other kits, which may be due to the different principle of rRNA depletion or the amount of input total RNA. For seriously degraded RNA from FFPE samples, libraries could be constructed with as low as 50 ng of total RNA, although there was higher residual rRNA and lower unique mapping ratio in the libraries. Conclusions: These results indicated that most of clinical FFPE samples could be used in the RNA profiling study. The method of RNA library construction with rRNA depletion can be used for clinical FFPE samples. For degraded and low-input RNA samples, it is still possible to obtain repeatable RNA expression profiling but with a low unique mapping ratio and high residual rRNA.

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Feldmann, Klaus, Matthias Weber und Wolfgang Wolf. „Design of a theoretical holistic system model as base of construction kits for building Plug&Produce-able modular production systems“. Production Engineering 1, Nr. 3 (07.09.2007): 329–36. http://dx.doi.org/10.1007/s11740-007-0052-6.

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Parkinson, Eric F. „An Examination of the Interaction Between Modelling and Its Relationship with Construction Kits: Lessons from the Past and for the Future“. International Journal of Technology and Design Education 14, Nr. 3 (2004): 219–43. http://dx.doi.org/10.1007/s10798-004-1577-z.

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Shuhaibar, M., C. Walsh, F. Lindsay, N. Lee, P. Walsh, P. O’Gorman, G. Boran et al. „A comparative study of faecal occult blood kits in a colorectal cancer screening program in a cohort of healthy construction workers“. Irish Journal of Medical Science 180, Nr. 1 (17.10.2010): 103–8. http://dx.doi.org/10.1007/s11845-010-0605-0.

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Jaskólski, Mariusz. „Integrating biocrystallography into traditional biology and chemistry curricula“. Journal of Applied Crystallography 34, Nr. 3 (22.05.2001): 371–74. http://dx.doi.org/10.1107/s0021889801003624.

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New academic courses for teaching protein crystallography to biology and chemistry students have been developed. The general aim of the lecture courses is to introduce crystallographic terminology and modern diffraction methodology, to discuss the principles of macromolecular structure, to develop confidence in assessing macromolecular models, and to develop skills in extracting biostructural information from crystallographic literature as well as from bioinformatics resources available on the Internet. Emphasis on structural biology (chemists) or on crystallographic concepts and methodology (biologists) differs depending on the background of the students. In practical classes, the students work with real models of crystals and lattices, and use construction kits to build models of protein secondary structure. Diffraction images of oriented protein crystals are used for determination of unit-cell parameters. An Internet-based multiple-choice quiz is used for tests, self-assessment and practice.

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