Dissertationen zum Thema „Cologne water“
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Pålsson, Ale. „Our Side of the Water : Political Culture in the Swedish colony of St Barthélemy 1800–1825“. Doctoral thesis, Stockholms universitet, Historiska institutionen, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-133528.
Der volle Inhalt der QuelleFreire, Elionai Gomes. „5/6 nephrectomy and its influence on permeability water and eltrÃlitos in the colon of rats agreed.5/6 nephrectomy and its influence on permeability water and eltrÃlitos in the colon of rats agreed“. Universidade Federal do CearÃ, 2016. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=17608.
Der volle Inhalt der QuelleThe nephrectomy 5/6 is a surgical procedure most commonly used in physiology laboratories to reproduce chronic renal failure due to its reproducibility and practicality. Patients with IR or nephrectomized present hemodynamic disorders such as fluid overload and accumulation of azotÃnicas substances in plasma, which can have serious complications in various organs. The aim of this research was to evaluate the changes that nephrectomy 5/6 can cause colonic permeability of rats to water and electrolytes (Na+,K+,Cl-) after 3,7 and 14 days of renal failure The project was approved by CEUA-UFC protocol with n 11/15. 92 animals were used (male rats Wistar, weighing 280-300 g) divided into three groups 3 days post-nephrectomy 5/6 7-days nephrectomy 5/6 and 14 days after nephrectomy 5 / 6- each group being formed by a SHAM and an experimental. For studies of colonic permeability of water and electrolytes, the animals were anesthetized with urethane, and then subjected to colonic infusion Tyroad + phenol red for 60 min. For all experiments, we monitor hemodynamic parameters (systolic-PAS blood pressure, central venous pressure, PVC, often heart-FC and blood volume- VS) and also biochemical plasma concentrations of urea and creatinine. Our biochemical results of bun and serum creatinine in groups 7 and 14 days confirmed the renal failure after nephrectomy 5/6 (114,8Â1,8dl/ml; 165,6Â7,2dl/ml) e (0,95Â0,02dl/ml; 1,3 0,07dl/ml) respectively. Groups 7 and 14 days post-nephrectomy 5/6 also showed significant blood volume changes (11,12Â0,37 ml) e (15,83Â0,82ml) The group 3 days did not present biochemical or significant hemodynamic results and insignificant colonic secretion of water and electrolytes (Na+,K+,Cl-) Correlation by linear regression curves between the colonic secretion of water and electrolytes in the blood volume in the group 7days performed quite significant in this work. We conclude that the 5/6 nephrectomy proved effective in chronic renal failure model, as promoted successive rise in urea levels and serum creatinine. The nephrectomy 5/6 promoted colonic secretion into water and electrolytes becoming evident in rats in groups 7 and 14 days post-nephrectomy. The close relationship of blood volume changes in group 7 days with the secretion of water and electrolytes (Na+, k+, Cl-) It makes us deduct be plasma hyperglycemia induced by 5/6 nephrectomy the decisive for absorption and secretion of these ions or in the colon.
Léonard, Clément. „Etude d'une colonne à bulles pour le traitement d'effluents par oxydation en voie humide“. Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4382.
Der volle Inhalt der QuelleWet Air Oxidation (WAO) is a process dedicated to degrade the organic matter contained in wastewater with the help of an oxidation reaction. WAO works at high pressure (10 – 30 MPa) and high temperature (373 – 613 K) using oxygen from air as oxidiser. WAO processes are carried out in bubble column reactors in order to maximise both the liquid residence time and the mass transfer of oxygen from the gas to the liquid, one of the key element of the process. The lack of available data and correlations, needed for the prediction of the parameters governing mass transfer in bubble columns working in WAO conditions, is then penalising. Measurements of gas holdup, bubble diameters and interfacial area in clear water and in presence of a pollutant (phenol), in reactive and non-reactive conditions, show major effects of superficial gas velocity, phenol concentration and saturation of the gas by water vapor. The primary bubble, formed at the gas distributor, and the column diameter, are important design parameters for the process optimisation. Measurement of the mass transfer coefficient by a new chemical technique using phenol oxidation shows that it depends primarily on the bubble diameter and on the gas and liquid velocities. This experimental work is completed by the development of correlations of the key parameters in WAO conditions, providing relevant elements for the design of WAO units working in continuous mode
Glinghammar, Björn. „Toxicological aspects of bile acids and human fecal water on cultered human colon carcinoma cells /“. Stockholm, 2001. http://diss.kib.ki.se/2001/91-628-4816-x/.
Der volle Inhalt der QuelleLEVANTI, CRISTINA. „Computational fluid dynamics and experimental study of the hydrodynamics of a bubble column and an air-water jet-stirred cell“. Doctoral thesis, Università degli Studi di Cagliari, 2013. http://hdl.handle.net/11584/266103.
Der volle Inhalt der QuelleMaquin, Mathilde. „Développement d'un modèle hydrologique de colonne représentant l'interaction nappe - végétation - atmosphère et applications à l'échelle du bassin versant“. Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS286/document.
Der volle Inhalt der QuelleThe representation of the water cycle on land surfaces is essential for climate modeling. Nowadays, the "Land Surface Models" (LSMs) represent soil columns of a few meters deep and they simulate the temporal evolution of the vertical water flows and the interaction with the atmosphere. However, the interaction with a near-surface water table is not taken into account although it strongly influences the evapotranspiration fluxes at the local scale, and therefore the climate at the regional scale. This interaction, which occurs at a smaller scale than the grid scale of the LSMs, is difficult to model. The objective of this PhD is to propose a model that incorporates the impact of a near-surface water table on evapotranspiration fluxes for global climate models. The computation time of the model must be small enough to enable simulations at large spatial and temporal scales. In this context, a new soil column model is proposed with a drainage function that is imposed at the bottom of the column. This function aims at reproducing the temporal evolution of the water table level in interaction with both the infiltration and the evapotranspiration fluxes. The model is tested and validated on numerical experiments and on a real catchment (Strengbach, France). A methodology based on this column model is introduced to estimate the evapotranspiration fluxes taking into account their subgrid variability. This methodology is applied to a catchment whose area is similar to the one of a classic grid cell of LSMs (Little Washita, USA)
Lopez-Ibanez, Manuel. „Operational optimisation of water distribution networks“. Thesis, Edinburgh Napier University, 2009. http://researchrepository.napier.ac.uk/Output/3044.
Der volle Inhalt der QuelleMoul, Ian E. „Environmental contaminants, disturbance and breeding failure at a great blue heron colony on Vancouver Island“. Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29592.
Der volle Inhalt der QuelleLand and Food Systems, Faculty of
Graduate
Windt, Benjamin [Verfasser], und Walter [Akademischer Betreuer] Hermanns. „Colon-Biopsien beim Hund : Histopathologie und klinische Befunde in einer retrospektiven Studie / Benjamin Windt ; Betreuer: Walter Hermanns“. München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1185978941/34.
Der volle Inhalt der QuelleMhadhbi, Takoua. „Dynamique des pesticides au sein d’un écosystème lagunaire (lagune de Bizerte, Tunisie) : identification des sources de contaminations et processus de transformation des contaminants“. Thesis, Montpellier, 2019. http://www.theses.fr/2019MONTG040.
Der volle Inhalt der QuelleThe Bizerte Lagoon watershed is surrounded by intense agricultural activities. The use of phytosanitary products to treat the crops that surround this watershed, causes a deterioration of the lagoon water quality. Monitoring the level of pollution in this ecosystem requires the application of combined approaches. Thus, in this work a survey is carried out to determine the sources of pollution due to agricultural activities and to identify pesticides used by farmers. To verify the presence of the molecules determined during the investigation, a diagnosis of water column pollution is carried out using two sampling techniques: grap sampling and passive sampling (POCIS). Sediments are also threatened by these xenobiotics, thus, a screening was carried out in this matrix. It is relevant to determine the fate of these pollutants once they are present in the aquatic environment. Thus, the study of their photodegradation in the water column was carried out. The degradation of these pollutants can give rise to by-products that are more toxic than the parent molecules. An ecotoxicological approach is developed in this work to determine the toxicity of these products on a model species Ostreococcus tauri
AMALFITANO, STEFANO. „Structure and function of benthic microbial community in highly variable freshwater systems“. Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2008. http://hdl.handle.net/2108/576.
Der volle Inhalt der QuelleSalvia, Marie-Virginie. „Développement d’outils analytiques et méthodologiques pour l’analyse et le suivi de composés vétérinaires et stéroïdes hormonaux à l’état de traces dans l’eau et le sol“. Thesis, Lyon 1, 2013. http://www.theses.fr/2013LYO10036/document.
Der volle Inhalt der QuelleSeveral chemical products are dispersed in the environment and the consequences can be sometimes harmful for humans and the ecosystems. Among these substances appear the antibiotics and the hormonal steroids. Nowadays, only few data are available on the presence and the fate of these substances in the environment in particular for solid matrices, mainly due to a lack of methodologies. Consequently, methods to analyze traces of « emergent » contaminants in water and soil were carried out. Therefore, multi-residues and inter-families procedures based on LC-MS/MS analysis were established. Concerning the aqueous samples, 23 analytes are extracted with the SPE technique (OASIS HLB). MLQs are between 0.09 and 34 ng/L. For the solid matrix, the extraction procedure of 31 compounds is inspired from the method called QuEChERS and followed by a purification step. This methodology was validated and MLQs between 0.013 and 3 ng/g were obtained. Two antibiotics families, tetracyclines and fluoroquinolones, were studied separately as they have specific physical/chemical properties and are therefore difficult to extract from soil with an inter-families method. Then, the method developed for the soil matrix allowed a statistic study which showed the impact of the soil parameters on the recoveries and matrix effects. Finally, the methodologies were applied to a soil column study which allowed obtaining data on the transfer, accumulation and degradation of the substances in soil
Boyd, Timothy David. „The Novel Use of Recombinant Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) to Reverse Cerebral Amyloidosis and Cognitive Impairment in Alzheimer’s Disease Mouse Models: Insights from the Investigation of Rheumatoid Arthritis as a Negative Risk Factor for Alzheimer’s Disease“. Scholar Commons, 2010. http://scholarcommons.usf.edu/etd/3571.
Der volle Inhalt der QuelleHassen, Barkai Allatchi. „Caractérisation d’un écoulement diphasique dans un airlift sous dépression. Application pour l’extraction des matières solides en suspension“. Thesis, Lyon, 2020. http://www.theses.fr/2020LYSEI038.
Der volle Inhalt der QuelleBubble columns are gas-liquid contactors widely used in industry, especially in chemical, biological and mineralogical process applications. In the range of bubble columns available, the vacuum airlift is a French innovation with very interesting characteristics for hydraulic pumping, mass transfer and suspended matter separation. This thesis work is part of the development of this improved airlift, which is widely used in the industrial environment. The objective of the thesis is the hydrodynamic characterization of the airlift column under vacuum and analysis of its capacities to ensure the solid-liquid separation function. This work is exclusively experimental and the experimental setup is a vertical bubble column in plexiglass under vacuum and connected to a recirculation basin. The hydrodynamic analysis was carried out using a differential pressure sensor for the global study and using a double optical probe for local characterization. Results obtained made it possible to study flow regime. The main parameters obtained are the void fraction, superficial velocity and bubbles diameter. Particle Image Velocimetry is applied to visualize and analyze the flow structures in the recirculation basin. The extracting capacities of the column were studied in tap water with the addition of surfactants. Soluble casein and Methyl Iso Butyl Carbinol (MIBC) are the two surfactants that have given the best results in terms of solid-liquid separation of suspended matter. This work contributed to the understanding hydrodynamics for vacuum airlift column and helped to highlight the potential of this column in the solid-liquid separation process. This work also opens the way to numerical modelling of airlift column hydrodynamics from experimental results
Lamouret, Marie. „Traitement automatisés des données acoustiques issues de sondeurs multifaisceaux pour la cartographie des fonds marins“. Electronic Thesis or Diss., Toulon, 2022. http://www.theses.fr/2022TOUL0002.
Der volle Inhalt der QuelleAmong underwater acoustic technologies, multibeam echo sounder (MBES) is one of the most advanced tool to study and map the underwater floors and the above water column. Its deployment on-site requires expertise so as the whole data processing to map the information. These processing are very time-consuming due to the massive quantity of recorded data and thus needs to be automatised to shorten and alleviate the hydrographer's task. This PhD research works focus on the automatisation of the current activities in Seaviews society.After some reminders on the underwater acoustic sciences, the MBES operating is described as well the produced data that will be manipulated throughout the developments. This document presents two thematics˸ bathymetric (depths) and marine habitats mapping. The developments are integrated into the Seaviews' software in the aim to be used by all the employees.About seafloor depths mapping, the bathymetric sounding has to be sorted to avoid that the outlier errors distort the results. Sorting the uncountable measures is cumbersome but necessary, although the hydrographers are today happily computed-assisted. We propose a fast statistical method to exclude the outliers while mapping the information. This leads to wonder if the water column imagery would be workable to deduce the bathymetry without failure. We will test this hypothesis with some technics of deep learning, especially with convolutional neural networks.The marine habitats mapping is a seabed nature classification according to the local life. Seaviews has worked on a way to prepare MBES data and habitats analysis. Concerning the method of classification itself, we move towards machine learning technics. Several methods are implemented and assessed, and then an area is chosen to evaluate and compare the results
Lin, Bo-Feng, und 林栢楓. „Automatic Pressurized Water Reactor Loading Pattern Design Using Ant Colony Algorithms“. Thesis, 2010. http://ndltd.ncl.edu.tw/handle/27737032660517787589.
Der volle Inhalt der Quelle國立清華大學
核子工程與科學研究所
98
In a pressurized water reactor (PWR), the fuel assemblies must be reloaded after operating a period of time. Loading pattern (LP) was designed by engineer based on experience. Because the computer capability is greatly improved in recent years, it is possible to search loading pattern using some algorithms. Ant Colony System is a metaheuristic method which is efficient to combinatorial optimization problem. In this study, three kinds of algorithms, i.e., Rank-Based System (RAS), Max-Min Ant System (MMAS), Ant-Q was adopted to search loading pattern of PWR. Maanshan nuclear power plant cycle 17,18 and 19 was applied to demonstrate the capability of the algorithms. Safety and economy must be considered in loading pattern design. The main safety constraints include hot channel factor (FΔH) and moderator temperature coefficient (MTC) and the economic consideration is cycle length and cost of fuel. The LP was mirror symmetry in quarter-core and quarter-core rotational symmetry in the full core. The search procedure contained two steps. The first step was to permute fuel assemblies (FA) in 1/8 region and the second step was to perform rotation configuration of these FAs. The developed program was coded with C. SIMULATE-3 code was used to calculate core status. In Maanshan Cycle 19 case, most of the loading patterns searched by RAS, MMAS and Ant-Q satisfied the safety limits. Generally speaking, MMAS had better performance. The results of Maanshan Cycle 17, 18 also satisfied safety limits, which showed this tool can be applied to other cycles. In addition, the design whose four FAs were placed by lower enrichment FAs also reached the target cycle length. Also, parallel computing was tested to reduce computation time.
SHIH, SHAN-JIN, und 石善瑾. „Solving Water Vehicle Routing Problem by Ant Colony Optimization and Tabu Search Algorithms“. Thesis, 2019. http://ndltd.ncl.edu.tw/handle/65stwv.
Der volle Inhalt der Quelle國立臺北科技大學
工業工程與管理系
107
Our economy has grown more vigorously than ever before, Taiwan’s industrial has been accompanied by pollution and damage to water resources which affect the source of drinking water for the people. Therefore people have the purchasing behavior of water, and finally derived the drinking water transportation industry for the people. Due to the different frequency and capacity of demand in various regions, how to effectively distribute is an important issue. This research uses P Company in Taichung as an example to define a case study as a vehicle defined as Period Vehicle Routing Problem (PVRP) and Heterogeneous Fixed Fleet Vehicle Routing Problem (HFFVRP). In the course of the process, construct a mathematical model based on PVRP and HFFVRP, solve the initial scheduling by Insertion Methods and The Nearest Neighbor Methods, improve the cycle problem by the Tabu Search (TS), and finally solve the distribution route by using the Ant Colony Optimization (ACO). The goal is to minimize the driving cost so that the operator can efficiently plan the distribution route and the distribution sequence of each demand point.
Shang, Chih-Jen, und 尚至仁. „An Ant Colony System Approach for Wafer Probing Scheduling Problems“. Thesis, 2008. http://ndltd.ncl.edu.tw/handle/97452891849548486813.
Der volle Inhalt der Quelle華梵大學
工業工程與經營資訊學系碩士班
96
This study applied an ant colony system (ACS) to solve the wafer probing scheduling problem (WPSP). WPSP is a variation of the parallel machine scheduling problem (PMSP) with sequence dependent setup time (SDST) and due date restriction. The objective of this study is minimizing the total setup time without violating any constrains. To evaluate the performance of ACS, 480 instances were generated based on the characteristics of a test floor. The computational results showed that ACS not only outperformed WSA for each testing instance groups, but also reduced number of machine used. Therefore, it is believed that the proposed ACS is an effective method to solving WPSPs.
Chen, Ying-Hsiu, und 陳盈秀. „Automatic Pressurized Water Reactor Loading Pattern Design Using Ant Colony Algorithms and Tabu Search“. Thesis, 2013. http://ndltd.ncl.edu.tw/handle/78436953723877859936.
Der volle Inhalt der Quelle國立清華大學
工程與系統科學系
101
In a pressurized water reactor (PWR), the fuel assemblies must be reloaded after operating for one and half year, and thus the loading pattern (LP) needs to be redesigned to achieve the longest cycle length and satisfy the safety requirements. The LP design is accomplished by engineers based on their experience. Since the computer capability has been greatly improved, designing the LP by computer become possible. Our laboratory had applied Ant Colony Algorithms to automatically design the LP a couple years ago, but the time effort still need to be improved. In this research, the computational time that the LP design takes can be dramatically reduced by combining Ant Colony Algorithms and Tabu Search (TS). This research use C language to write the program and simulate the core status by SIMULATE-3 under UNIX environment. There’re two stages to design a LP. First, search a rather good fuel loading pattern using Ant Colony Algorithm and 2D SIMULATE-3 calculation. Second, adjust the loading pattern to fulfill the requirements using Tabu Search. There’re two ways to adjust the LP: one is “swap”, the other is “rotation”. The experimental results show that a loading pattern design can be accomplished automatically within a rather short time.
Wang, Cheng-Der, und 王政德. „Automatic Boiling Water Reactor Loading Pattern & Control Rod Pattern Design using Ant Colony Optimization Algorithm“. Thesis, 2011. http://ndltd.ncl.edu.tw/handle/97648675744553560153.
Der volle Inhalt der Quelle國立清華大學
工程與系統科學系
99
The reload design of a boiling water reactor (BWR) consists of fuel loading pattern design and control rod pattern design. The fuel loading pattern design is to permute the fuel assemblies so that shutdown margin requirement is fulfilled and the thermal limit margin is good enough to guarantee the satisfactory control rod pattern design. The control rod pattern design is to determine the inserted depth of control rods at each exposure point so that the thermal limits such as minimum critical power ratio (MCPR), maximum linear heat generation rate (MLHGR), and maximum average planar linear heat generation rate (MAPLHGR) meet the margin requirement and shutdown margin and cycle length are fulfilled. Automatic design of boiling water reactor loading pattern and control rod pattern were developed using the rank-based ant system (RAS) which is a variety of ant colony optimization (ACO) algorithm. The ACO algorithm is an effective optimization algorithm for combinatorial optimization problem and the heuristic rules of ACO algorithm were adopted to reduce search space and computation time. In loading pattern design, to reduce design complexity, fuel assemblies (FAs) were chosen to load the positions of one-eighth core geometry using probabilistic solution construction of ACO algorithm and then the corresponding fuel assemblies were loaded into the other part of the core. When the pattern was determined, Haling cycle length, the thermal limits at the end of cycle, and beginning of cycle (BOC) shutdown margin (SDM) were calculated using SIMULATE-3 code, which were used to evaluate the loading pattern for updating pheromone concentration of ACO algorithm. In control rod pattern design which followed either the A2-B1-A1-B2 or A1-B2-A2-B1 sequence in this study. After the control rod pattern was determined, the axial power distribution, effective multiplication factor (keff), shutdown margin, and three thermal limits were calculated using SIMULATE-3 code, which were then used to evaluate the control rod pattern and update the pheromone concentration. The developed design methodology was demonstrated using two fuel reload cycle of Kuosheng nuclear power plant. The results show that the designed satisfactory reload design with an acceptable cycle length can be achieved within a reasonable computation time.
Yen, Wei-Ting, und 顏維廷. „Automatic Search of the Power Ascension Path for a Boiling Water Reactor Using Ant Colony Optimization Algorithm“. Thesis, 2009. http://ndltd.ncl.edu.tw/handle/36802118254866442070.
Der volle Inhalt der Quelle國立清華大學
工程與系統科學系
97
During power ascension in boiling water reactor, both economic benefits and operation safety must be considered. For economic consideration, it should bring up the power to the full power level as soon as possible. As for the operation safety, it should ensure the fuel rod integrity under any operation condition and thus have to satisfy all the operation safety limits, including minimum critical power ratio, maximum linear heat generation rate, maximum average planar linear heat generation rate, fuel envelope, power oscillation, and so forth. For the time being, this task is designed by nuclear engineers who use POWERPLEX-III to figure out control rod withdrawal, xenon waiting, and watching the margin of the operation safety limits during the power ascension, which must be finished before start up operation. The goal of this research is to use ant colony optimization algorithm to search the power ascension path, in which using power search mode of SIMULATE-3 to calculate core status. In the algorithm, solution component set contains all the possible action, i.e., control rod withdrawal and core flow rate increment. To consider the thermal limits, power oscillation, maximum control rod line, envelope and so forth during the path search, those terms were formulated as the penalty terms of the quality function. The heuristic information was used to eliminate the unreasonable action when constructing solution. Using SIMULATE-3 to calculate the core status along the power ascension path, and then the quality function was calculated. The pheromone concentration was updated according to the quality function value, until the program converge to a best solution. The developed program was demonstrated by two cycle and the results show that the goal was achieved.
Hsieh, Ming-Chun, und 謝明君. „Spinal p90 ribosomal S6 kinase 2 (RSK2) mediates water avoidance stress-induced colon-bladder crosstalk in rats“. Thesis, 2012. http://ndltd.ncl.edu.tw/handle/08765426989169900795.
Der volle Inhalt der Quelle國立中興大學
獸醫學系暨研究所
100
Clinical evidences revealed an overlap of neural mechanisms mediated by bowel and lower urinary tract (LUT) dysfunctions, a phenomenon known as cross-organ sensitization (COS). Although studies have been demonstrated that stress could modulate the neurotransmission of visceral sensation, through the colonic afferent pathway, the effects of stress on COS have yet been established. To clarify whether chronic stress was involved in the colon-bladder crosstalk induction or/and maintain, we performed cystometrogram in response to intracolonic mustard oil (MO) instillation in female Wistar-Kyoto rats exposed to daily water avoidance stress (WAS, 1 hour/day for 10 days) or sham treatment (WASham). In addition, Quantitative real-time PCR (Q-PCR), Western blot, Immunoprecipitation (IP) and Immunfluorescence (IF) were used to assess the protein/mRNA expression at the lumbosacral dorsal horn (L6-S1). WAS demonstrated the enhanced colonic motility, but no effect on the baseline voiding function and time-dependent enhancement spinal p90 ribosomal S6 kinases 2 (RSK2) protein/mRNA levels in spinal cord neuron. Intracolonic MO dose-dependently decreased intrercontraction intervals (ICIs) and threshold pressure (TP) accompanied with enhanced spinal phosphorylated RSK2 (pRSK2) and phosphorylated NR2B (pNR2B) expression as well as RSK2-PSD-95 and PSD-95-NR2B copreciptitaion in WAS but WAsham animals. Intrathecal kaempferol (a RSK2 antagonist, 10uM, 10ul), bilateral adrenalectomy and subcutaneous RU38486 (a glucocorticoid antagonist, 40 mg/kg) prevented MO-induced bladder hyperactivity and attenuated the said protein/mRNA expression and coprecipitation in WAS group. Taken together, these results suggest spinal RSK2 activation downstream stress-related glucocorticoid release from the adrenal cortex to mediate WAS-dependent sensitization of colon-bladder crosstalk via the spinal PSD-95/NR2B cascade, and offer the possibility of developing pharmacological strategies for stress-related pelvic pain.
YANG, JIA-RONG, und 楊佳蓉. „Comparison of Using Artificial Bee Colony and Genetic Algorithms to Search Glycine-Water Molecular Cluster Structure and pKa Values“. Thesis, 2019. http://ndltd.ncl.edu.tw/handle/q52ak6.
Der volle Inhalt der QuelleTSAI, YU-WEN, und 蔡毓文. „Inhibitory effects of water extracts of grey and white maitake (Grifola frondosa) on cell migration of the human colon adenocarcinoma cell line“. Thesis, 2018. http://ndltd.ncl.edu.tw/handle/r9ezub.
Der volle Inhalt der Quelle亞洲大學
食品營養與保健生技學系
106
According to statistics from the Health promotion Administration, Ministry of Health and Welfare, colorectal cancer is the most commonly occurring cancer in Taiwan. About 10,000 people get colorectal cancer each year, and about one in five patients are in the fourth phase. Invasion and metastasis of cancer cells are often one of the major causes of cancer death and lead to poor prognosis. Invasion is related to the breakdown of cell stroma, cell attachment and mobility changes. Studies have found that fruiting bodies of Grifola frondosa and mycelium are rich in nutrients, which have significant effects on diabetes, hypertension, hyperlipidemia, etc., in which the polysaccharide contained in Grifola frondosa has the immunity in the stomach and anti-tumor effect. In this experiment, grey and white Grifola frondosa water extracts with invasive human colorectal cancer cell line LoVo, respectively, 24,48 hours MTT assay and find a safe dose to wound healing assay and transwell migration two an experiment was conducted to evaluate the ability of Grifola frondosa water extracts to inhibit the metastasis of colorectal cancer cells. The experimental results showed that after LoVo cells were cultured for 24,48 hours under different doses of grey and white Grifola frondosa water extracts, the survival rate of LoVo cells decreased significantly with the increase of the extract content, The Grifola frondosa water extracts safe concentration of 0.8 mg / mL or less. In the wound healing assay and transwell migration, the two kinds of Grifola frondosa water extracts were treated for 48 hours, respectively, showing dose-effect, and two kinds of Grifola frondosa water extracts significantly inhibited cell creep distance and transwell migration degree. Western blot analysis also showed that Grifola frondosa water extracts can reduce the protein expression of N-cadherin, β-catenin, Vimentin, Snail and Slug, which are involved in the process of EMT. From the above experimental results we can see that the low concentration of grey and white Grifola frondosa water extracts rough extract effectively inhibit the function of LoVo metastasis may have the ability to assist the treatment of metastatic colorectal cancer.
Liao, Pei-Chi, und 廖珮綺. „Examination of species composition and nitrate transporter gene sequences by single-colony sequencing in the diatom genus Skeletonema collected in Matsu waters“. Thesis, 2016. http://ndltd.ncl.edu.tw/handle/e5fa75.
Der volle Inhalt der Quelle國立臺灣海洋大學
海洋生物研究所
105
Skeletonema is a dominant diatom genus in the coastal waters near Matsu Islands. Skeletonema contains nitrate transporter genes (Nrt2), the expression level of which reflects the physiological status of this diatom in the utilization of ammonium and nitrate. In order to correctly interpret metatranscriptomes of phytoplankton in Matsu waters and to design highly efficient primers for quantitative polymerase chain reaction (qPCR), single-colony sequencing was employed to identify genes belonging to the same species within Skeletonema. In June and July 2016, plankton samples were collected at several stations near Nangan Island, Matsu. From these samples, 12 Skeletonema colonies were isolated with a micromanipulator. Next, the total amount of DNA in each colony was increased by multiple substitution amplification (MDA). Using the amplified DNA as templates, gene fragments of 18S rRNA, rbcL, Nrt2 and EFL were cloned by PCR and sequenced. Among them, 18S rRNA and rbcL sequences were used to confirm the taxonomical association of individual colonies, and the corresponding Nrt2 and EFL sequences were regarded as genes belonging to the verified species. In the 12 Skeletonema colonies, a total of 48 sequences were obtained for genes mentioned above. According to 18S rRNA and rbcL sequences, there were 7 colonies identified as S. subsalsum and the other 4 colonies were S. tropicum. Each colony was then sequenced to reveal Nrt2 sequences. The results showed that S. subsalsum and S. tropicum each contained two forms of homologous Nrt2 genes. As for the reference gene, only one EFL sequence was cloned from the S. tropicum coloies. In contrast, EFL sequences belonging to S. subsalsum could be categorized into four groups on the phylogenetic tree. Through these sequencing efforts, sequences from individual Skeletonema colonies were used to identify genes of unknown species in metatranscriptomes, and species-specific qPCR primers could be designed with more confidence.
Reddy, Manne Janga. „Swarm Intelligence And Evolutionary Computation For Single And Multiobjective Optimization In Water Resource Systems“. Thesis, 2006. https://etd.iisc.ac.in/handle/2005/370.
Der volle Inhalt der QuelleReddy, Manne Janga. „Swarm Intelligence And Evolutionary Computation For Single And Multiobjective Optimization In Water Resource Systems“. Thesis, 2006. http://hdl.handle.net/2005/370.
Der volle Inhalt der Quelle„Effects of scutellariae radix extract and its major flavonoid baicalein on electrolyte transport across human colonic epithelia (T84 cells)“. 2003. http://library.cuhk.edu.hk/record=b5891544.
Der volle Inhalt der QuelleThesis (M.Phil.)--Chinese University of Hong Kong, 2003.
Includes bibliographical references (leaves 113-120).
Abstracts in English and Chinese.
Abstract (English version) --- p.i
Abstract (Chinese version) --- p.iii
Acknowledgements --- p.v
Table of contents --- p.vi
List of figures --- p.x
List of tables --- p.xiii
List of abbreviations --- p.xiv
Chapter Chapter I: --- Introduction --- p.1
Chapter 1.1. --- Transepithelial electrolyte transport in colon --- p.1
Chapter 1.1.1. --- Intestinal fluid secretion --- p.1
Chapter 1.1.2. --- Cellular mechanism of chloride secretion --- p.3
Chapter 1.2. --- Biological activities of flavonoids --- p.6
Chapter 1.2.1. --- Classification and general activities of flavonoids --- p.6
Chapter 1.2.2. --- Bioavailability and pharmacokinetic properties of flavonoids --- p.8
Chapter 1.3. --- "What is Scutellariae radix""?" --- p.9
Chapter 1.3.1. --- Usage in Traditional Chinese Medicine --- p.9
Chapter 1.3.2. --- Relationship with Coptidis rhizoma --- p.9
Chapter 1.4. --- Effect of flavonoids on gastrointestinal activities --- p.12
Chapter 1.4.1. --- Genistein and quercetin --- p.12
Chapter 1.4.2. --- Baicalein --- p.12
Chapter 1.5. --- Possible intracellular signaling pathway involved in the secretory response by Scutellariae radix (SR) in T84 cells --- p.14
Chapter 1.5.1. --- Human colonic T84 cell --- p.14
Chapter 1.5.2. --- Intracellular signaling pathway --- p.14
Chapter 1.6. --- Aim of study --- p.17
Chapter Chapter II : --- Methods and Materials --- p.18
Chapter II.1. --- Culture technique of the T84 cells --- p.18
Chapter II.2. --- Simultaneous measurement of short-circuit current (Isc) and intracellular calcium ([Ca2+]i) --- p.21
Chapter II.2.1. --- Experimental setup --- p.21
Chapter II.2.2. --- Preparation of the permeable supports --- p.23
Chapter II.2.3. --- Cell seeding --- p.27
Chapter II.2.4. --- Dye loading --- p.27
Chapter II.2.5. --- Simultaneous measurement of Isc and [Ca2+]i- --- p.30
Chapter II.3. --- Conventional short-circuit current (Isc) measurement --- p.34
Chapter II.3.1. --- Experimental setup --- p.34
Chapter II.3.2. --- Preparation of the permeable supports --- p.36
Chapter II.3.3. --- Cell seeding --- p.36
Chapter II.3.4. --- Measurement --- p.38
Chapter II.4. --- Measurement of cAMP --- p.39
Chapter II.5. --- Solutions and chemicals --- p.40
Chapter II.6. --- Statistical analysis --- p.42
Chapter Chapter III : --- Results --- p.43
Chapter III. 1. --- Effects of baicalein and its interaction with calcium and cAMP-dependent secretagogues --- p.43
Chapter III. 1.1. --- Effects of baicalein on baseline Isc and [Ca2+]i --- p.43
Chapter III. 1.2. --- Ionic basis of baicalein-evoked Isc --- p.43
Chapter III. 1.3. --- Effect of baicalein on carbachol-evoked Isc --- p.47
Chapter III. 1.4. --- "Effect of baicalein on Isc stimulated by another calcium mobilizing agonist, histamine" --- p.58
Chapter III. 1.5. --- Effect of carbachol on Isc response stimulated by baicalein --- p.61
Chapter III. 1.6. --- Chronic effect of baicalein on carbachol-evoked increase in Isc --- p.63
Chapter III.1.7. --- Interaction of baicalein with forskolin --- p.65
Chapter III.2. --- Effects of baicalein on cAMP generation in T84 cells --- p.69
Chapter III.2.1. --- Effects of baicalein on cAMP production --- p.69
Chapter III.2.2 --- Effects of baicalein on forskolin-induced cAMP production --- p.70
Chapter III.3. --- Effects of Scutellariae radix extract on ion transport activities in T84 cells --- p.73
Chapter III.3.1. --- Effects of Scutellariae radix extract (SRE) on baseline Isc --- p.73
Chapter III.3.2. --- Ionic basis of SRE-evoked Isc --- p.77
Chapter III.3.3. --- Effects of adenylate cyclase inhibitor and PKA inhibitor --- p.77
Chapter III.3.4. --- PKC modulation --- p.86
Chapter III.3.5. --- Involvement of intracellular calcium --- p.86
Chapter III.3.6. --- Involvement of cAMP --- p.94
Chapter Chapter IV : --- Discussion --- p.98
Chapter IV. 1. --- Effects of baicalein on ion transport in human colonic T84 cells --- p.98
Chapter IV. 1.1. --- Roles of baicalein in chloride secretion in intestinal epithelial cells --- p.98
Chapter IV. 1.2. --- Potentiation effect of baicalein on calcium-mediated chloride secretion --- p.100
Chapter IV. 1.3. --- Potentiation effect of carbachol on baicalein-stimulated chloride secretion --- p.102
Chapter IV. 1.4. --- Interaction between baicalein and forskolin --- p.104
Chapter IV.2. --- Effects of Scutellariae radix extract on ion transport in human colonic T84 cells --- p.107
Chapter IV.2.1 --- Characteristcs of Isc induced by Scutellariae radix extract --- p.107
Chapter IV.2.2. --- Possible signaling mechanism involved in Isc induced by Scutellariae radix extract --- p.108
Chapter IV.3. --- Comparison of the effects on ion transport in human colonic T84 cells produced by baicalein and Scutellariae radix extract --- p.110
Chapter IV.3.1. --- Properties of baicalein- and Scutellariae radix extract- induced Isc response --- p.110
Chapter IV.3.2. --- Summary --- p.111
Chapter Chapter V : --- References --- p.113
Publications --- p.120