Dissertationen zum Thema „Co-Culture systems“
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Dongre, Arundhati. „Modelling lymphangioleiomyomatosis (LAM) using two-dimensional and three-dimensional in vitro co-culture systems“. Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/50037/.
Der volle Inhalt der QuelleOstertag, Renate Magdalene [Verfasser]. „Characterisation of human co-culture systems for applications in bone tissue engineering / Renate Magdalene Ostertag“. Mainz : Universitätsbibliothek Mainz, 2013. http://d-nb.info/1041898878/34.
Der volle Inhalt der QuelleFreyer, Nora [Verfasser]. „Optimizing culture conditions for hepatic differentiation of human induced pluripotent stem cells : from 3D culture systems to co-cultures / Nora Freyer“. Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2018. http://d-nb.info/1160514968/34.
Der volle Inhalt der QuelleZhiyan, Wu. „The co-creation and circulation of brands and cultures : historical Chinese culture, global fashion systems, and the development of Chinese global brands“. Thesis, University of Exeter, 2010. http://hdl.handle.net/10036/3165.
Der volle Inhalt der QuelleDijamentiuk, Alexis. „Propagation de communautés bactériennes : modelage, stabilisation et sélection pour la biopréservation“. Electronic Thesis or Diss., Université de Lorraine, 2023. http://www.theses.fr/2023LORR0124.
Der volle Inhalt der QuelleRecent discoveries about microbial communities, or microbiota, have revealed considerable biotechnological potential in a variety of fields. They are considered essential to accelerate innovation in food production systems. However, existing processes are not adapted to the cultivation of microbiota. One major barrier to community propagation is competition between microorganisms, which can lead to an undesirable reduction in biodiversity within the culture reactor. This phenomenon can lead to communities that lack the desired functionality. The objective of this thesis was to study the influence of microbiota propagation, under controlled conditions, on their structure and function. During this work, a process of microbial culture excluding microbial competition for the propagation of bacterial communities was developed. The chosen strategy is based on the micro-confinement and spatial segregation of bacteria within a broth structured as an invert emulsion. The effect of the invert emulsion culture on the growth of individual bacteria was studied, then the effect of this system on the dynamics of communities propagated according to a sequential regime, or backslopping, as well as that exerted by a conventional non-emulsified system was investigated. The results showed that the use of an inverse emulsion leads to the generation of new community structures during propagation, and that the use of the classical culture leads to their stabilization. The different behaviors of these two culture systems make them complementary tools for the modeling and the propagation of microbial communities. Finally, the effect of propagation on the functional variability of communities was studied in a biopreservation context. The screening of propagated raw milk microbiota showed that they differed in terms of robustness and reproducibility of anti-Listeria activity, emphasizing the need to take into account the functional variability of communities when selecting communities of interest for microbiota engineering
Kanjickal, Deenu George. „Perivascular Drug Delivery Systems for the Inhibition of Intimal Hyperplasia“. University of Akron / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=akron1133715441.
Der volle Inhalt der QuelleHammoudi, Taymour Marwan. „3D micropatternable hydrogel systems to examine crosstalk effects between mesenchymal stem cells, osteoblasts, and adipocytes“. Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/45972.
Der volle Inhalt der QuelleEssaouiba, Amal. „Development of a liver-pancreas in vitro model using microfluidic organ-on-chip technologies“. Thesis, Compiègne, 2020. http://www.theses.fr/2020COMP2573.
Der volle Inhalt der QuelleDiabetes mellitus (DM) or the so called disease of the century is a life threatening dysfunction that affects the endocrine system. The mechanisms underlying the break in the feedback loop that regulates the metabolism and the consequent diabetes induction are not fully known. Understanding the mechanisms of insulin action is therefore crucial for the further development of effective therapeutic strategies to combat DM. Accordingly, it is imperative to find a robust and reliable model for diabetes research able to overcome the limitations of traditional 2D in vitro cell culture and animal experimentation. The aim of this thesis is to develop a new liver‐pancreas co‐culture model using advanced microphysiological systems (MPs) to tackle more effectively the mechanism involving the hepatic and pancreatic endocrine regulation. This work highlights the power of multi organ‐on‐chip systems that combines the advanced 3D‐cell compartmentalization, microfluidics and induced pluripotent stem cells (iPSC) technology to achieve a high biological complexity and functions that are rarely reproduced by only one of these tissue engineering technologies
Hammond, John Stotesbury. „Scaffolds for liver tissue engineering : in vitro co-culture & in vivo release“. Thesis, University of Nottingham, 2009. http://eprints.nottingham.ac.uk/12556/.
Der volle Inhalt der QuelleSmith, A. S. T. „Development and characterisation of a novel myotube-motoneuron 3D co-culture system“. Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1339141/.
Der volle Inhalt der QuelleThomas, Robert James. „Formation, morphology and function of a 3D hepatocyte-hepatic stellate cell co-culture system“. Thesis, University of Nottingham, 2006. http://eprints.nottingham.ac.uk/11964/.
Der volle Inhalt der QuelleEfremova, Liudmila [Verfasser]. „Development of neuron-astrocyte co-culture system for mechanistic and pharmacological studies in neurodegeneration / Liudmila Efremova“. Konstanz : Bibliothek der Universität Konstanz, 2015. http://d-nb.info/1081016922/34.
Der volle Inhalt der QuelleRodger, Andrew Neil Sutherland. „Sea-based integrated multi-trophic aquaculture : investigation of a fish, bivalve and macroalgal co-culture system“. Thesis, University of the Highlands and Islands, 2010. https://pure.uhi.ac.uk/portal/en/studentthesis/seabased-integrated-multitrophic-aquaculture(cf63b33d-c09c-449d-a501-215df54b9395).html.
Der volle Inhalt der QuelleScheiblich, Hannah Christina [Verfasser]. „Nitric oxide (NO)- and carbon monoxide (CO)-mediated signal transduction in a co-culture system of microglia and human model neurons / Hannah Christina Scheiblich“. Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2015. http://d-nb.info/108086783X/34.
Der volle Inhalt der QuelleRoberts, Jessica L. „Development of an ex-vivo co-culture system to model pulpal infection by Streptococcus anginosus group bacteria“. Thesis, Cardiff University, 2010. http://orca.cf.ac.uk/55492/.
Der volle Inhalt der QuelleBowler, Laura. „Pseudomonas aeruginosa biofilm and planktonic bacteria display different virulence mechanisms when co-cultured with human A549 lung cells using the Calgary Biofilm Device co-culture system“. American Society for Microbiology, 2012. http://hdl.handle.net/1993/31262.
Der volle Inhalt der QuelleMay 2016
Dixon, David A. „Development of a Co-culture System to Mimic the Transfection of HSV-1 from Keratinocytes to Neuronal Cells“. Wright State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1401894059.
Der volle Inhalt der QuelleMocherla, Supriya. „Inhibitory Effects of Growth Factors on Proliferation of Porcine Smooth Muscle Cells in the Direct Co-culture System“. VCU Scholars Compass, 2007. http://scholarscompass.vcu.edu/etd_retro/142.
Der volle Inhalt der QuelleSieh, Shirly. „Development of a 3D culture system to study the skeletal metastasis of prostate cancer“. Thesis, Queensland University of Technology, 2011. https://eprints.qut.edu.au/50870/1/Shirly_Sieh_Thesis.pdf.
Der volle Inhalt der QuelleTse, Pui-keung. „An investigation on the conversion of C3 to embryotrophic iC3b in the human oviductal cell-mouse embryo co-culture system /“. View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36357601.
Der volle Inhalt der QuelleTse, Pui-keung, und 謝沛強. „An investigation on the conversion of C3 to embryotrophic iC3b in the human oviductal cell-mouse embryo co-culture system“. Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B45010936.
Der volle Inhalt der QuelleAlradi, Fahad Mohammed. „The Response of Unpolarized Macrophages (RAW 264.7)/Keratinocytes (PAM-212) Monolayer and Co-Culture System to Herpes Simplex Virus Type 1 (HSV-1) Replication during the Infection“. Wright State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=wright1525337119493727.
Der volle Inhalt der QuelleSchmohl, Michael [Verfasser], und Stefan [Akademischer Betreuer] Stevanovic. „Analyzing biological activity of drugs- Inter- and intracellular signaling analysis within an organotypic co-culture system / Michael Schmohl ; Betreuer: Stefan Stevanovic“. Tübingen : Universitätsbibliothek Tübingen, 2011. http://d-nb.info/1162699302/34.
Der volle Inhalt der QuelleLarsen, Hege Ekeberg. „Neuronal control of cardiac excitability in pro-hypertensive states“. Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:04d38eb6-ea66-4283-91ab-dd75bca246e9.
Der volle Inhalt der QuelleKöhl, Vera [Verfasser]. „Characterization of a novel Lin-CD34+CD133+CD41+ HSPC population in Myelofibrosis patients and establishment of a long-term co-culture system / Vera Köhl“. Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2021. http://d-nb.info/1236695267/34.
Der volle Inhalt der QuelleWasilewski, David [Verfasser]. „Establishing a neuron-astrocyte co-culture system to model non-cell autonomous mechanisms of neurotoxicity and synaptotoxicity in the context of Alzheimer’s disease / David Wasilewski“. Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2020. http://d-nb.info/123415031X/34.
Der volle Inhalt der Quellevan, der Merwe Marnus. „Integrating aquaculture with crop systems : an aquaponic enterprise project proposal for the Ntinga Multipurpose Co-Operative in Philippi, South Africa“. Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/96858.
Der volle Inhalt der QuelleENGLISH ABSTRACT: Stellenbosch University was approached to assist with developing a techno-financial model for an urban freshwater aquaculture system in Philippi, Cape Town. Rapidly growing urban areas are predominantly becoming concentrated zones for malnutrition and poverty which require attention. Having enough food to eat does not mean that a family is food secure, the problem is usually associated with the lack of access to nutritious food. Fish is seen as an extremely healthy food which has the potential to effectively support food security and alleviate malnutrition. Aquaculture is identified as a largely underdeveloped sector in South Africa. It is currently undergoing rapid transition, being promoted by government as an industry that has potential to develop and create jobs, provide food security and grow the South African economy. Aquaponics- a method to integrate aquaculture with growing crops in a symbiotic system is a highly resource efficient closed-integrated food producing technology which has the potential to benefit from South African biosecurity regulations and climate-geographic characteristics. It is viewed as an effective food production alternative to deal with the challenges of declining high quality freshwater resources and available arable land. Training and capacity building is important for the development of aquaponic technology. This study explores and identifies the advantages aquaponic technology development would have in South Africa. The study has reviewed and assessed the fundamental principles for aquaculture production and management required for aquaponic systems development and management. A practical case study identifies the daily challenges and design parameters of aquaponic systems. The study is concluded with a techno-financial project proposal which shows how aquaponic systems can be planned.
AFRIKAANSE OPSOMMING: Universiteit Stellenbosch was genader om 'n tegno-finansiele model to ontwikkel vir 'n stedelike akwakultuur plaas in Philippi, Kaapstad. The tempo waarteen die stedelike areas groei ontwikkel kommerwekkende uitdagings soos wanvoeding en armoede. In hierdie studie is vis geindentifiseer as 'n uiters voedsame aanvulling in die dieet van Suid Afrikaners. Akwakultuur is grootliks agter in terme van ontwikkeling. Dit word beskou as 'n sektor wat groot potensiaal inhou vir Suid Afrika se eknomiese groei, werkskepping en voedselsekuriteit. Akwaponika is die hersirkulerende integrasie van akwakultuur en hidroponika. Akwaponika hou groot voordele in terme van Suid Afrika se biosekuriteit regulasies and geografiese eienskappe en is 'n effektiewe manier om gebruikte akwakultuur te suiwer. Opleiding en beplanning word gesien as 'n fundamentele benadering tot suskesvolle akwaponika ontwikkeling. Hierdie studie bestudeer die Suid Afrikaanse omgewing en potensiaal vir akwaponika ontwikkeling. Die fundamentele beginsels van akwakultuur en hidroponika bestuur en produksie is saamgesit wat beskou word as die aanbevele manier om akwaponika te bestuur. 'n Praktiese gevallestudie toon die daaglikse uitdagings aan en gee raad oor daaglikse bestuur van akwaponika stelsels. Die studie word afgesluit met 'n tegno-finansiele model wat wys hoe om 'n akwaponika sisteem te beplan.
Tran, Anh Hien. „Bacterial biofilm treatment and in situ antimicrobial coatings for orthopaedic implant retention surgery“. Thesis, Queensland University of Technology, 2021. https://eprints.qut.edu.au/207850/1/Anh%20Hien_Tran_Thesis.pdf.
Der volle Inhalt der QuelleTylek, Tina [Verfasser], Jürgen [Gutachter] Groll, Franz [Gutachter] Jakob und Andreas [Gutachter] Beilhack. „Establishment of a Co-culture System of human Macrophages and hMSCs to Evaluate the Immunomodulatory Properties of Biomaterials / Tina Tylek ; Gutachter: Jürgen Groll, Franz Jakob, Andreas Beilhack“. Würzburg : Universität Würzburg, 2021. http://d-nb.info/1232647586/34.
Der volle Inhalt der QuelleVasanthi, Bathrinarayanan Pranav. „Evaluating the biological effects of electronic cigarettes using a novel in-house designed aerosol delivery system and an in-vitro co-culture model of the human airways“. Thesis, Aston University, 2018. http://publications.aston.ac.uk/37691/.
Der volle Inhalt der QuelleBerry, Jennifer N. „THE MESOCORTICOLIMBIC DOPAMINE PATHWAY RECONSTITUTED IN VITRO: GLUTAMATE RECEPTORS AND CORTICOSTEROID-METHAMPHETAMINE NEUROTOXICITY“. UKnowledge, 2013. http://uknowledge.uky.edu/psychology_etds/28.
Der volle Inhalt der QuelleBoulestreau, Yann. „Une démarche de co-conception d’innovations du système de culture au système agri-alimentaire pour une gestion agroécologique des bioagresseurs telluriques en maraîchage provençal Analyzing barriers and levers for practice change: a new framework applied to vegetables’ soil pest management“. Thesis, Avignon, 2021. http://www.theses.fr/2021AVIG0725.
Der volle Inhalt der QuelleA rapid and far-reaching change towards farming practices that contribute to the protection of the environment and the human health is needed. In many cases, these alternative practices exist but are not implemented due to interconnected barriers at the plot, farm, territory, value chain and/or global level. In my thesis, I developed a methodology taking into account the determinants of the farming practice choices at the different levels to support the change in farming practices. I applied this methodology to a specific case study: the management of soil-borne pests and diseases, mainly root-knot nematodes, in sheltered vegetable farming systems in Provence (France). The impact of root-knot nematodes on vegetable crops is significant both in Provence (40% of farms affected) and worldwide. Their management is essentially based on the use of non-selective nematicides that are damaging for the human health and the environment.First, I carried out a sociotechnical analysis showing that most of the Provençal agri-food system was locked around the use of "radical soil disinfection" techniques, thus excluding the implementation of alternative agroecological techniques. This lock-in arose from interconnected barriers to the change in practices, involving a diversity of stakeholders at the Provençal level and beyond it: farmers, upstream and downstream of the sector (including consumers), R&D and public policy actors. Following this analysis, I studied existing coupled innovations that foster the implementation of agroecological crop protection in French vegetable systems. This “tracking of innovations” led us to identify 5 types of coupled innovations, and for each of them, the combinations of sociotechnical levers mobilized and the way they were implemented. Meanwhile, I developed a serious game enabling the effective sharing of the sociotechnical analysis results to the stakeholders of the studied problem. This serious game also enabled to facilitate stakeholders’ knowledge management and creativity and the collaboration between them, for initiating the design of innovative solutions tailored for the problem under study. Finally, I mobilized the previous works (analysis, tracking, serious game) in 4 co-design workshops conducted with the stakeholders. I created and implemented several methods in these workshops to design increasingly elaborate solutions that favor change in practices. As a result, we collectively designed 50 coupled innovations including 41 coupled innovations, thus opening up the space of possible solutions. We evaluated part of the complex coupled innovations.In the discussion, I point out the promising avenues of action and research to facilitate the implementation of agroecological practices for the management of soil-borne pests and diseases in Provençal sheltered vegetable farming systems. I discuss the possible evolution of the methodology I developed during this thesis, in order to improve its efficiency and complete the design process. I make proposals to specify the conditions of the implementation of the innovations designed, evaluate them and anchor them in the territorial agri-food system. Finally, I show that this work contributes to establishing theoretical and methodological bases to multi-level redesign of agricultural systems for accompanying changes in farming practices.Parts A "Problem" and C "Discussion" of my thesis are written in French. Part B consists of three articles and one chapter of the thesis written in English
Franzén, Ähdel Carina, und Wilén Frida Bulukin. „Alla vill varandras väl : Hur ledarskap genom medarbetarskap i en kvalitetskultur skaparpatientnöjdhet vid svenska sjukhus“. Thesis, Mittuniversitetet, Avdelningen för kvalitets- och maskinteknik, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:miun:diva-38812.
Der volle Inhalt der QuelleCommitted leadership is the foundation for creating a good quality culture andto succeed, the participation of employees is required. Researchers argue thatsupport for the current leaders in healthcare has until now been ratherundeveloped. The purpose of this study was to understand how the qualityculture in Swedish healthcare can be linked to the leaders' ability to promotegood co-workership. This study was based on an explanatory sequentialmixed method with two quantitative measurements that ultimately resulted in aqualitative interview. A measurement of the quality culture at Swedishhospitals was taken based on a previously developed instrument formeasuring quality culture . The measuring instrument comprises 13behavioral pairs that promote or hinder a quality culture. This measure showsthat there is generally a good quality culture at Swedish hospitals at present.Through this measurement, a regression analysis was done which links to thehospital's results in the National Patient Survey. A statistically significantbehavior was observed, and according to this measurement, is likely to createmore satisfied patients as more professions feel that this behavior occurs intheir organization. The behaviour in question can be described as: when wehave a problem, we find out the root cause before we decide on a solution.This behavior was brought into two successful hospitals in order to understandmore deeply how they work with the co-workership in this particular behavior.Based on the workshop with these two hospitals, we conclude that leadersneed to have experienced the quality culture and the co-workership they areassumed to carry in order to promote an employee culture that is linked to thequality culture. In pursuit of success, a strong quality culture is desirable, butbased on the workshops, the authors see no possible quick solution to reachit. The result shows that today's leaders in healthcare need support in the formof a mentor or time for reflection on leadership on their own or in groups inorder to develop. In constructive approach, it is with improvement knowledgethat everyone's involvement in the work of continuous improvement isconducted. This requires reflection, honesty, courage, openness and trustfrom everyone involved and intentions rooted in the wellness and prosperity ofall.
2019-06-27
Banerjee, Nivedita. „Systematic Approach to Compare the Inflammatory Response of Liver Cell Culture Systems Exposed to Silver, Copper, and Nickel Nanoparticles“. Thesis, 2010. http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8414.
Der volle Inhalt der QuelleChu, Heng-Chang, und 朱恆昌. „Establishment of co-culture bacterial system for biohydrogen production“. Thesis, 2015. http://ndltd.ncl.edu.tw/handle/72493196996395097247.
Der volle Inhalt der Quelle國立中興大學
生命科學系所
103
Biofuel production is using renewable biomass as substrate to produce fuels such as hydrogen,ethanol and butanol. Lignocellulose is the most abundant renewable feedstock to produce biofuel. Lignocellulose is mainly composed of cellulose, hemicelluloses and lignin. However, glucose-based is surrounded by hemicellulose, lignin and pectin, which make it hard to be decomposed. This study was aimed to establish a functional bacterial consortia as a lignocellulose digestion system for biofuel production through serial repeated batch culture. The major bacterial composition of batch culture were termite gut isolated Bacillus pumilus MGB 5 and Clostridium acetobutylicum ATCC 824. This system does not require sterilization, aeration and shake.On an eight day incubation period the system could produce 275 ml /L H2, ethanol 1250 mg/L, butanol 71 mg/L, acetate acid 2275 mg/L, butyrate acid 661 mg/L and left 64% cellulose and hemicellulose from mango powder biomass. The major bacteria in these system are Enterobacteriaceae, Enterococcus, Clostridium, Enterobacter, Bacillus. In the experiment , we found out if we use 3% mango powder as carbon source which can produce higher butanol than using 1% glucose as carbon source.This simple system is very potential to replace the traditional system which use glucose as carbon source to produce hydrogen and butanol.
Lien, Po Min, und 連博民. „High-efficiency co-immobilized microorganism culture system using loofah sponge for ethanol production from rice straw in a modified co-culture cell bioreactor“. Thesis, 2013. http://ndltd.ncl.edu.tw/handle/48894884981852204350.
Der volle Inhalt der Quelle長庚大學
生化與生醫工程研究所
101
In recent years , cellulosic ethanol is the one of renewable energy that can replace tranditional petrochemical fuel . Agricultural wastes contains large amounts of cellulose , hemicellulose , lignin and other components , cellulose and hemicelluloses can transform to glucose and xylose by hydrolyzing . Furthermore , it can produce bioethanol . The initial flask experiments , it shows that the ethanol yield is 3.18 g / L , the conversion rate was 62.4% when the rotention speed is 75 rpm , the ratio of Z. mobilis and P. stipitis is 1: 3 , and separated-immobilization of fermentation conditions . Then , we use rice straw as substrate , to discuss aeration rate, stirring speed and other factors , expect to get the best ethanol yield and metabolic rate . The experiment will be simultaneous saccharification and co-fermentation (SSCF) in reactor to investigate the high substrate concentrations and repeated batch . At present , the initial concentrate in addition to the 10 g / L rice straw , we also discuss 20 g / L and 30 g / L . The results show that the initial substrate concentration is higher , the lower the rate of ethanol conversion . And in the experiment ,we found that the substrate concentration of 30 g / L, the bottom of the aerobic zone caused by congestion due to rice straw powder , this problem will be used repeated batch culture to improve .In hydrolyzate discuss, we add 10 g/L rice straw as raw material which the medium after over-liming , the ethanol yield can be achieved 814 ppm. After then, the repeated-bacth fermentation medium
Zhong, Ming Hong, und 鍾明宏. „Real-time impedimetric monitoring of cell proliferation in a co-culture system“. Thesis, 2016. http://ndltd.ncl.edu.tw/handle/32622609462939283550.
Der volle Inhalt der QuelleLiu, Yu An, und 劉祐安. „A high-efficiency co-immobilized microorganism culture system using barium alginate for ethanol production from rice straw in a modified co-culture cell bioreactor“. Thesis, 2011. http://ndltd.ncl.edu.tw/handle/36904166154002653160.
Der volle Inhalt der Quelle長庚大學
化工與材料工程學系
99
Swelling and disruption of Ca-alginate immobilization carrier by aeration and agitation are inevitable in the co-culture system that cause low production rate. In this study, we improved the carrier materials and develop a suitable gel matrix for the long-term immobilization culture of strains and established the optimal operating conditions for high-efficient ethanol production using a modified bioreactor. Our results showed that Ba-alginate (10% w/v) carrier had better enzyme activity than Ca-alginate carrier in the flask experiments using Carboxymethylcellulose (CMC). Under the optimal operation conditions of Ba-alginate 4%、aeration rate 1 vvm、agitation rate 150 rpm、2 day pre-culture, our modified co-culture cell bioreactor had ethanol production concentration of 778 ppm/10g-CMC at 8 hr with the theoretical maximum yield of 15.6%. Among the different pretreatment substrates of rice straw, the ethanol concentration was 600 ppm at 7 hr with the theoretical maximum yield of 24% and 1254 ppm at 29 hr with the theoretical maximum yield of 16.7% for 10 g/L and 30 g/L rice straw substrates, respectively. In a repeat-batch culture conditions with the total pretreatment rice straw concentration of 20 g/L, the total ethanol production can reached 1002 ppm at 34 hr, the yield for three batches were about 24%, 12%, 18%, respectively.
Yu, Hao-Hsin, und 游晧欣. „Neuroprotection of recombinant arginine deiminase (rADI) in a neurons and microglia co-culture system“. Thesis, 2007. http://ndltd.ncl.edu.tw/handle/49951953781523785835.
Der volle Inhalt der Quelle國立臺灣大學
藥學研究所
95
Nitric oxide (NO●) plays double-edged roles in human, not only important in physiological functions but also involved in many pathological pathways. Particularly, the overproduction of NO● generated by inducible nitric oxide synthase (iNOS) is associated with many neuronal disorders such as Parkinson’s disease, Alzheimer’s disease, and cerebral ischemic neuronal damage, etc. Arginine deiminase (rADI), expressed in some microorganisms but not in mammalian cells, can catalyze L-arginine (L-arg) to L-citrulline (L-cit). ADI has been reported the inhibitory activity of iNOS-mediated NO●production. A co-culture of BV2 (a murine microglial cell line) and SHSY5Y (a human neuroblastoma cell line) was established to understand the role of rADI in iNOS-mediated neurotoxicity. The co-culture was treated with the combination of 2 μg/mL of lipopolysaccharide (LPS) and 1 ng/mL of interferon-γ (IFN-γ) for 2 days, and successfully induced iNOS-mediated neuronal death. In the co-culture system, 1 mU/mL of purified recombinant ADI (rADI) was administrated for 2 and 3 days into the co-culture with LPS/IFN-γ treatment. The cell viability and NO● production were measured by MTT assay and Griess method, respectively. In addition, the specific neuronal viability and functionality were analyzed by neuron-specific immunostaining and 3H-dopamine uptake assay, respectively. The results showed that rADI treatment significantly preserved the cell viability (89.2±2.2 % of the initial cells) and decreased the NO● production (19.5±5.5 μM) on day 2, compared with the cells with LPS/IFN-γ treatment only (21.1±4.1 % and 67.0±1.3 μM, respectively) by MTT assay and Griess assay. Furthermore, the results of immunostaining showed that rADI treatment substantially preserved the neurons, and the dopamine uptake function was also maintained on day 2 (from 35.7±11.4% to 103.0±12.6 % of the initial cells) by rADI treatment. In addition, the neuroprotection of 3-day rADI treatment was observed better than 2-day treatment on day 4 by MTT assay (76.8±11.7 and 42.7±2.0 % of the initial cells, respectively), and dopamine uptake assay (75.1±10.8 and 34.5±10.5 % of the initial cells, respectively). To understand the possible neuroprotection mechanism of rADI treatment, replenishment of L-arg into the co-culture system with rADI treatment was conducted. Besides, the treatments of 1400W (a selective iNOS inhibitor) and vinyl-L-NIO (a selective neuronal NOS (nNOS) inhibitor) in the co-culture system were also performed. The results showed that the replenishment of L-arg abolished the neuroprotective and NO● suppressive effect of rADI in the co-culture system. In addition, the treatment 1400W preserved the cell viability (EC50=2.3 μM) and inhibited NO● production (IC50=5.7 μM), but vinyl-L-NIO did not. The results indicated that the neuroprotective mechanism of rADI is via L-arg depletion which inhibits the NO● production produced by iNOS, but not nNOS. According to the accumulative results, the conclusions are that rADI can protect the neurons from LPS/IFN-γ induced neurotoxicity in the co-culture system. rADI not only preserves the neuron viability but also maintains the functionality. The protection mechanism of rADI may be via depletion of L-arg and subsequently inhibits the iNOS mediated NO● production. To evaluate the therapeutic role of rADI in iNOS mediated neuronal disorders, further investigations in the neuroprotection of rADI in primary cell and ischemic mice model are needed in the future.
Yu, Hao-Hsin. „Neuroprotection of recombinant arginine deiminase (rADI) in a neurons and microglia co-culture system“. 2007. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-1707200714450500.
Der volle Inhalt der QuelleBin-Han, SHIEH, und 謝秉翰. „Establish of a co-culture model system for studying melanosomes transfer from melanocytes to keratinocytes“. Thesis, 2005. http://ndltd.ncl.edu.tw/handle/93638055630479739037.
Der volle Inhalt der Quelle國防醫學院
生物及解剖學研究所
93
The type and amount of melanin is packaged into melanosomes in melanocyte then transferred to the neighboring 36 keratinocytes to modulate skin color. However, the actual processes involved in this transfer have not been defined clearly. Various methods have been used before to study the melanosomes transfer, for examples: (1) by using scanning electron microscopy to observe the melanosomes transfer;(2) by using protease inhibitor to verified melanosome transfer mechanism in vivo with histological examination;(3) labeling melanocytes with fluorescing dye (succinimidyl ester of carboxyfluorescein diacetate (CFDA)) or gold dextrin then co-cultured with keratinocytes to implicate melanosome transfer activity. The methods mention above are all complicated and time consuming. Therefore, establish a simple, rapid, direct and reliable method to examine specific transfer of melanosme from melanocyte to keratinocytes is very important for screening inhibitory compounds from Chinese herb to modulate skin color.In this study, a co-culture system of melanocytes and keratinocytes was established and the melanosome transfer activity was examined by triple labeling of immunofluorescence. The number of keratinocytes with melanosome after co-culture of keratinocytes and melanocytes with serine protease inhibitor AEBSF for 72 hours or with MSH for 24 hours was analysed by flow cytometry and confocal microscopy. The data indicated that the co-culture model system combined with triple labeling of immunofluorescence to observe the melanosomes transfer activity from melanocytes to keratinocytes was feasible.
Hsuan, Yu Ching, und 余婧萱. „The study of Trichomonas vaginalis and human cervicalcancer cell line co-culture system in vitro“. Thesis, 2006. http://ndltd.ncl.edu.tw/handle/42865686564459375202.
Der volle Inhalt der Quelle國立成功大學
微生物暨免疫學研究所
95
Trichomonas vaginalis, a protozoan parasite of the urogenital -vaginal tract, is the causative agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in human. In male, the trichomoniais is usually asymptomatic, although it may cause irritating urethritis or prostatitis. In female, trichominiasis is associated with a wide spectrum of clinical signs ranging from a relatively asymptomatic state to severe vaginitis with a foul-smelling vaginal discharge. T. vaginalis may act as a potential catalyst in the acquisition of secondary infection such as that caused by human papillomavirus, the organism responsible for the pathogenesis of cervical cancer. The adherent clump of this protozoan will destruct the epithelial cell and induce pathogenesis by contact-dependent cytotoxicty. A co-culture system of T. vaginalis and human cervical epithelium cancer cell line (Z172 cell) has been established in this study. Both of the protozoan and host cell grow well in the same culture condition and atmosphere. When Z172 cell exposure under T. vaginalis attack, the morphology of the host cells become round shape, shrinkage, detach, and part of the cells are died. The single Z172 cell is easier attacked by T. vaginalis then colonial cells. The more co-culture time and the more adhesion rate were observed in this study. After 12 hours’ co-culture, the adhesion between protozoan and cell become the most significantly than other time points observation. Does the pathological changes of the Z172 cell are derived by the physical or chemical after the adhesion of T. vaginalis are needed to more studies in the future.
Yu, Ching-Hsuan, und 余婧萱. „The study of Trichomonas vaginalis and human cervical cancer cell line co-culture system in vitro“. Thesis, 2006. http://ndltd.ncl.edu.tw/handle/27932175958729992129.
Der volle Inhalt der Quelle國立成功大學
微生物及免疫學研究所
95
Trichomonas vaginalis, a protozoan parasite of the urogenital -vaginal tract, is the causative agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in human. In male, the trichomoniais is usually asymptomatic, although it may cause irritating urethritis or prostatitis. In female, trichominiasis is associated with a wide spectrum of clinical signs ranging from a relatively asymptomatic state to severe vaginitis with a foul-smelling vaginal discharge. T. vaginalis may act as a potential catalyst in the acquisition of secondary infection such as that caused by human papillomavirus, the organism responsible for the pathogenesis of cervical cancer. The adherent clump of this protozoan will destruct the epithelial cell and induce pathogenesis by contact-dependent cytotoxicty. A co-culture system of T. vaginalis and human cervical epithelium cancer cell line (Z172 cell) has been established in this study. Both of the protozoan and host cell grow well in the same culture condition and atmosphere. When Z172 cell exposure under T. vaginalis attack, the morphology of the host cells become round shape, shrinkage, detach, and part of the cells are died. The single Z172 cell is easier attacked by T. vaginalis then colonial cells. The more co-culture time and the more adhesion rate were observed in this study. After 12 hours’ co-culture, the adhesion between protozoan and cell become the most significantly than other time points observation. Does the pathological changes of the Z172 cell are derived by the physical or chemical after the adhesion of T. vaginalis are needed to more studies in the future.
Demetropoulos, Carl Lee. „Enhanced production of Pacific dulse (Palmaria mollis) for co-culture with abalone in a land-based system“. Thesis, 2002. http://hdl.handle.net/1957/31533.
Der volle Inhalt der QuelleTsai, Ching-Wen, und 蔡靜雯. „CD44 expression of MSC and Cancer Cell or Fibroblast in 3D Co-culture System on Chitosan Film“. Thesis, 2013. http://ndltd.ncl.edu.tw/handle/13299022315318496906.
Der volle Inhalt der Quelle國立臺灣大學
醫學工程學研究所
101
In this study, we used mesenchymal stem cells (MSCs) 3A6, cancer cells SW620 and fibroblasts Hs68. First, we demonstrated that 3A6 is similar with MSCs by flow cytomerty analysis and differentiation assay. In addition, antigen markers were used to mark cancer stem cells. Chitosan is a natural, biodegradable, biocompatible, non-toxic and U.S. Food and Drug Administration (FDA) approved polysaccharide. In this study, chitosan was used as the coating substrates. When mesenchymal stem cells, cancer cells and fibroblasts were cultured on chitosan substrates, all cells became suspended and aggregated into spheroids. We used the antigens of transmembrane glycoprotein CD44 on the cell membrane to mark cells in order to compare the influence of different materials, and determine the causes of any discrepancies. Furthermore, we cultured MSCs, cancer cells, and fibroblasts on chitosan substrates in direct co-culture systems to explore the interactions between the three types of cells. We labeled these cells by the CellTrackerTM and used the inverted fluorescence microscope to observe the morphology and distribution of cells on substrates. In addition, we used confocal fluorescence microscope to check the arrangement of 3D spheroids. Besides, we used the antigens of transmembrane glycoprotein CD44 to mark cells in order to observe the effect of different substrates on the co-culture systems. Finally, we used the MTT assay, immunofluorescence staining Ki-67 and LIVE/DEADR Viability/Cytotoxicity Assay Kit to prove and discuss the status of cells on different positions of cell spheroids.
Hu, Po Tai, und 胡柏泰. „Development of Co-Culture System for Butanol Production from Pretreated Rice Straw in a Modified VMFB Bioreactor“. Thesis, 2016. http://ndltd.ncl.edu.tw/handle/41412917626321426423.
Der volle Inhalt der QuelleWu, Pin-Hsien, und 吳品嫻. „The Effect of Adipose-derived Stem Cell and Amniotic Membrane Co-culture System in Periodontal Bone Regeneration“. Thesis, 2013. http://ndltd.ncl.edu.tw/handle/26892536754437193767.
Der volle Inhalt der Quelle國立臺灣大學
口腔生物科學研究所
101
Periodontal disease is a chronic inflammatory reaction of periodontal tissues that potentially leads to tooth loss. It can affect daily activity, such as chewing and speech. Unfortunately, there is no effective therapy for periodontal disease. Use of tissue engineering technologies and stem cell therapy on Periodontal bone regeneration is a very promising treatment. Adipose-derived stem cell has been proved to its multipotency and have significant outcome in many disorders. Amniotic membrane is anti-inflammation, anti-angiogenic, low immunogenicity, and enriched with growth factor. We create a co-culture system with adipose-derived stem cell and amniotic membrane in periodontal osseous defect of rat model. The result suggest that the co-culture system could enhance periodontal bone regeneration. We believed this study could provide the base of the co-culture system as stem cell therapy and amniotic membrane transplantation in clinical periodontology.
Tylek, Tina. „Establishment of a Co-culture System of human Macrophages and hMSCs to Evaluate the Immunomodulatory Properties of Biomaterials“. Doctoral thesis, 2021. https://doi.org/10.25972/OPUS-20357.
Der volle Inhalt der QuelleDer Verlauf der angeborene Immunantwort auf Biomaterialien bestimmt maßgebend, ob das Material vom Körper angenommen wird, um so seine gewünschte Funktion zu erfüllen, oder ob es zur Einkapselung und im schlimmsten Fall zur Abstoßung kommt. Makrophagen spielen in diesem Prozess eine Schlüsselrolle, und ihr Polarisationszustand, entweder pro (M1), antiinflammatorisch (M2) oder ein dazwischenliegender Subtyp, ist dabei von entscheidender Bedeutung. Während ein vorübergehender proinflammatorischer Anfangszustand hilfreich ist, verschlechtert eine anhaltende Entzündung eine zeitnahe Heilung und die anschließende Regeneration. Daher könnte eine durch Biomaterialien beeinflusste Polarisation hilfreich sein, um die Makrophagen in die gewünschte Richtung zu lenken. Die in vivo Reaktion ist jedoch äußerst komplex und die Kultivierung von Makrophagen in vitro stellt nur einen Teil des Prozesses dar. An etablierten Co-Kultursystem zur Untersuchung der immunmodulierenden Eigenschaften von Biomaterialien mangelt es jedoch. Daher war es Ziel dieser Arbeit ein funktionelles Co-Kultursystem von humanen Makrophagen und humanen mesenchymalen Stromazellen (hMSCs) zu etablieren um die in vitro Bewertung der Immunantwort nach Kontakt mit Biomaterialien zu verbessern. Von Interesse sind hMSCs hierbei, da sie zusammen mit Makrophagen an der Geweberegeneration und an Entzündungsreaktionen beteiligt sind. Zudem weisen MSCs immunmodulierende Eigenschaften in Hinblick auf Makrophagen auf und sind aktiv am Verlauf der Fremdkörperreaktion nach der Transplantation von Biomaterial beteiligt. Im Rahmen dieser Arbeit wurden Poly(ε-caprolactone) (PCL)-Scaffolds auf Faserbasis als Biomaterialkonstrukte verwendet, welche mit der Technik des Melt Electrowriting (MEW) hergestellt wurden. Mit dieser Technik kann sowohl die Form der Scaffolds als auch die Porengröße variiert werden. Um Unterschiede der Scaffoldgeometrien und Porengrößen in Hinblick auf die Makrophagenreaktion zu untersuchen, wurden zunächst Versuche mit Makrophagen-Monokulturen durchgeführt. Zur Etablierung eines funktionellen Co-Kultursystem, wurde zu Beginn ein Aufbau für ein direktes und indirektes System in 2D erstellt. Dieser Aufbau wurde anschließend auf die Möglichkeit der Zell-Zell-Kommunikation darin analysiert. Weiterhin wurde ein, für beide Zelltypen, geeignetes Kulturmedium definiert, gefolgt von der Etablierung eines Protokoll für die Co-Kultivierung beider Zelltypen auf faserbasierten Scaffolds. Im Bezug zu dieser Arbeit wurden Scaffolds mit unterschiedlicher Geometrie mittels der Technik des Melt Electrowriting hergestellt um die Veränderung der Makrophagenpolarisation zu untersuchen. Dabei zeigte sich eine verstärkte M2-Polarisation auf Scaffolds mit einer kastenförmigen Morphologie, verglichen mit dreieckigen, runden oder ungeordnet-strukturierten Scaffolds. Die weitere Untersuchung von Scaffolds mit kastenförmigen Poren und präzisen Faserabständen von 100 µm bis zu 40 µm zeigte das kleinere Porengrößen die Elongation primärer menschlicher Makrophagen förderten. Begleitet wurde die verstärkte Elongation mit einer gesteigerten Polarisation in Richtung des M2 Typs. Dieser Effekt war nach Kultivierung von Makrophagen auf Scaffolds mit 40 µm Poren am stärksten ausgeprägt. Im Rahmen dieser Arbeit konnte damit erstmals eine länglichen Morphologie humaner Makrophagen mit einer Polarisierung in den M2 Typ korreliert werden. Diese Ergebnisse könnten daher für das Design neuer Biomaterialien, welche sich positiv auf die Geweberegeneration auswirken sollen, von Bedeutung sein. Die Zellkommunikation beider Zelltypen, welche über Mitochondrienaustausch im direkten und indirekten Co-Kultur-System nachgewiesen wurde, fand sowohl ausgehend von Makrophagen als auch von hMSCs statt. Dabei ermöglichten „Tunneling Nanotubes“ in der direkten Co-Kultur den Transfer von Mitochondrien von einem Zelltyp zum jeweils anderen, während in der indirekter Co-Kultur ein ungerichteter Transfer durch in das Medium freigesetzte extrazelluläre Vesikel (EVs) stattfand. Darüber hinaus wurde die phagozytotische Aktivität von Makrophagen nach Co-Kultivierung untersucht, um die immunmodulatorischen Eigenschaften von hMSCs nachzuweisen, wobei die höchste phagozytotische Aktivität nach 48 stündiger Co-Kultivierung festgestellt wurde. Da die üblicherweise verwendeten Serumzusätze für Makrophagen (humanes Serum (hS)) und hMSCs (fötales Kälberserum (FCS)) bei längerer Kultivierung den jeweils anderen Zelltyp nicht unterstützen konnten, wurden diese Seren durch humanes Thrombozytenlysat (hPL) ersetzt. Dieses erwies sich im Rahmen dieser Arbeit als optimale Ergänzung für die gemeinsame Kultivierung beider Zelltypen in der Co-Kultur. Dabei wurden der Phänotyp und die Populationsverteilung beider Zelltypen, sowie die phagozytotische Aktivität und die Veränderung des Genexpressionsprofils von Makrophagen untersucht und mit den jeweiligen Standard-Monokulturbedingungen verglichen. Des Weiteren konnte gezeigt werde, dass eine Zugabe von Heparin in Zellkulturen mit Makrophagen und hPL nicht nötig ist. Daher wurde auf den Zusatz von Heparin für alle weitere Experimente, die hPL und Makrophagen umfassten, verzichtet. Im letzten Teil der Arbeit wurde ein Protokoll für die Co-Kultivierung auf MEW Scaffolds erstellt. Neben der Etablierung eines Setups für die 3D-Kultivierung wurden sowohl Protokolle zur Bewertung phänotypischer als auch molekularer Veränderungen entwickelt. Durch Feststellung von Unterschieden in der Makrophagenreaktion in Abhängigkeit zu der Kultivierung mit / ohne hMSCs und entweder auf Scaffolds oder Plastik-Kulturschalen konnte die Funktionalität der Protokolle nachgewiesen werden. Mit dem in dieser Arbeit etabliertem funktionellen Co-Kultursystem von humanen Makrophagen und hMSCs können zukünftig Biomaterialien mit einem stärkeren in vivo -Bezug in Hinblick auf die Immunantwort bewertet werden. Darüber hinaus deuten Ergebnisse auf speziell konstruierte MEW-Scaffolds ein vielversprechendes Designkriterium für neu entwickelte Biomaterialien an, wobei die Polarisation der Makrophagen in Richtung des entzündungshemmenden, heilungsfördernden Typens durch eine gesteuerte Morphologieänderung beeinflusst werden kann. An diese Arbeit anschließende Experimente sollten sich auf die Untersuchung vielversprechender Scaffolds mittels Co-Kultivierung sowie auf die Anpassung der etablierten Protokolle an andere Biomaterialgruppen, wie beispielsweiße für Zemente oder Hydrogele, konzentrieren
Lee, Yi Chieh, und 李依潔. „The optimization of co-culture-microorganism system for ethanol production from rice straw using a verticle gravity bioreactor“. Thesis, 2014. http://ndltd.ncl.edu.tw/handle/08216933720512904849.
Der volle Inhalt der Quelle長庚大學
生化與生醫工程研究所
102
In this study, we developed a novel bioreactor that simultaneously enhanced the saccharification and fermentation process for bioethanol production. Initially, A. niger and T. reesei were co-immobilized on PU (polyurethane) carrier, and co-cultured in our bioreactor by using carboxymethylcellulose (CMC) as substrate, then Z. mobilis alginate beads were added into this bioreactor together to overcome the problem of simultaneous saccharification and bioethanol fermentation. By using pentose or hexose as subsdtrate, the effects of immobilization speed and inoculum ratio of Z. mobilis and P. stipitis on alcohol production in suspension cultivation were studied. The immobilization results showed that alcohol concentration was 2200 ppm when two strains co-immobilized, while alcohol concentration was 2485 ppm when two strains separately-immobilized. Z. mobilis and P. stipitis were competitive when they were co-immobilized in the same beads. The optimum speed results showed that higher speed had less beneficial effects on ethanol production when using Z. mobilis mono-cultivation fermentation. The higher speed had positive effects on ethanol production when using P.stipitis mono-cultivation fermentation .The inoculum ratio results showed that co-cultivation of Z:P=1:1 was better than mono-cultivation of Z:P=1:0. Our results also showed that the yield of the bioethanol could reach 737 ppm at 36 hours, when PU soaked with 10 g/L CMC in this co-culture system. The further results showed that the maximum ethanol concentration increased to 937 ppm when the CMC concentration was increased from 10 to 15 g/L.
chen, sky, und 陳彥勳. „Development of co-culture system for ethanol production from starch by Aspergillus awamori, Rhizopus japonicus, and Saccharomyces cerevisiae IR-2“. Thesis, 2007. http://ndltd.ncl.edu.tw/handle/88966592440635898633.
Der volle Inhalt der Quelle長庚大學
化工與材料工程研究所
95
Environmental pollution and agricultural wastes have spread all over the world in recent years so we, in order to do effective use of the offal. The purpose for study potapo starch to regard as substrate material and then utilile Aspergillus awamori and Rhizopus Japonicus of fungers to co-culture.It will make starch to come into being glucose after saccharifiction.Afterward, we use efficiency better saccharomycete- Saccharomyces cerevisiae IR-2 to research .Add S. cerevisiae IR-2 into the fungous co-suspension system in the best saccharification time (most reduce sugar), and let the system to become three strains of microorganisms co-suspension system.To find the suitable condition for culture the three strains of microorganisms system to go on different proportion and the amount of spores to addtion. At the same time,the system can avoid co-suspension during the saccharification process that accumulate the large amount reduce sugar make the damage of feedback inhibition,the saccharification and ferment processes can go on at the same moment. In the study,the best proportion to add A. awamor and R. japonicus is 7.5: 2.5,the large amount reduce sugar in the 28th hour and we get 2.152 mg/ml reduce sugar ,so we will go on next experiment that we find out the best addtion amount is 109 spores/ml to receive the concentration of best addtion by the experimental result. Follow-up the experiment , in which the best concentration for addtion is 0.1 x108 cell/ml on 12hr hour. It can get 0.57% of ethanol concentration. Transfer rotational speed for 100 rpm on 36th hour can up to 1.25%