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1

Bonilla-Lemus, Patricia, Saúl Rojas-Hernández, Elizabeth Ramírez-Flores, Diego A. Castillo-Ramírez, Alejandro Cruz Monsalvo-Reyes, Miguel A. Ramírez-Flores, Karla Barrón-Graciano, María Reyes-Batlle, Jacob Lorenzo-Morales und María Maricela Carrasco-Yépez. „Isolation and Identification of Naegleria Species in Irrigation Channels for Recreational Use in Mexicali Valley, Mexico“. Pathogens 9, Nr. 10 (07.10.2020): 820. http://dx.doi.org/10.3390/pathogens9100820.

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Members of the genus Naegleria are free-living amoebae that are widely distributed in water and soil environments. Moreover, Naegleria fowleri is a pathogenic amoeba species that causes a fatal disease in the central nervous system known as primary amoebic meningoencephalitis (PAM) in humans. Since most reported infections due to N. fowleri are reported in recreational waters worldwide, this study was aimed to describe the presence of these amoebic genus in Mexicali Valley irrigation channels of recreational use. A total of nine water samples were collected and processed by triplicate, in nine different sites of the Valley. After filtering and culturing the samples, plates were examined, and the observed amoebae were morphologically identified at the genus level. In addition, the pathogenicity of these amoebic isolates was checked, and molecular characterization was performed by PCR/sequencing. The results revealed the presence of Naegleria spp. in all the channels sampled. Finally, molecular identification confirmed the presence of five different species of Naegleria: N. fowleri, N. australiensis, N. gruberi, N. clarki and N. pagei. The presence of these protists, particularly N. fowleri, should be considered as a potential human health risk in the region.
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Koroleva, E. V. „Biological features of flowering of the genetic collection of plants of the family Onagraceae Juss. in the forest-steppe conditions of Western Siberia“. Проблемы ботаники Южной Сибири и Монголии 21, Nr. 2 (17.11.2022): 69–75. http://dx.doi.org/10.14258/pbssm.2022057.

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Data on the timing of the onset of phenological phases and the duration of the flowering period of the genetic collection of annual flowering plants from three sections of the family Onagraceae Juss. are presented: Rhodanthos, Godetia and Phaeostoma of the Clarkia Pursh. generic complex, outside their natural habitats (ex situ), on the collection site of the Novosibirsk State Agrarian University. As a result of the evaluation of the flowering phenomena of Clarkia sections, two flowering periods were identified. The species were distributed into summer-flowering plants C. purpurea from the Godetia section - with a vegetation duration of 102 ± 2 days and summer-autumn C. amoena and C. amoena subsp. Lindleyi from the Rhodanthos section with a vegetation duration of 123 ± 2 days and a species of C. unguiculata blooming throughout the period from the Phaeostoma section with a vegetation duration of 126 ± 2 days. Correlation analysis showed a high dependence of the growth and development periods of Clarkia species on the sum of biologically active temperatures > 10 °C (0.77 - 0.99). Cpurpurea from the Godetia section has the greatest adaptive potential to local climatic conditions, it requires the least amount of biologically active temperatures of the growing season (1903 °C) for growth and development than two other sections: Phaeostoma (2090 °C), Rhodanthos (2188 °C). Thus, all three sections of the Clarkia family Onagraceae Juss. they are characterized by prolonged flowering in the conditions of the forest-steppe of Western Siberia, are promising for urban gardening, differ in the possibility of sowing seeds in the open ground and are suitable for flower beds of continuous flowering.
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Koroleva, E. V. „Different quality of seeds of the new <i>Clarkia Amoena</i> raspberry bowl in the south of Western Siberia“. Bulletin of NSAU (Novosibirsk State Agrarian University), Nr. 2 (25.07.2023): 60–74. http://dx.doi.org/10.31677/2072-6724-2023-67-2-60-74.

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Clarkia amoena (Lehm.) A. Nelson & J. F. Macbr., a valuable outdoor ornamental crop, was grown south of Western Siberia (GPS coordinates: 55.0312880° N, 82.9903190° E). The work aimed to study the features of the reproductive biology of a new variety of clarkia Malinovaya chasha selected by the Novosibirsk State Agrarian University and to assess the topographic (quantity and quality of seeds from shoots of different orders), matural (various degrees of fruit ripening) and ecological (dependence of productive and sowing attributes on the main climatic factors in periods flowering and fertilisation) variability of economically valuable traits. The study used the method of multiple regression, correlation, and cluster analyses of experimental data. Fruits and seeds of a new variety of Clarkia Raspberry Cup, which were set during free pollination, formed at different flowering times and on shoots of various locations on the main stem, differed in quantity, weight, size and sowing qualities. Analysis of seed quality indicators (weight of 1000 seeds, germination energy, germination) revealed a multiple regression dependence on the heat supply of the periods of fertilisation and seed formation. The importance of the seeds of the clarkia variety Raspberry bowl by weight of 1000 pcs., formed in different periods, varied from 0.327 g in the period unfavourable for seed formation - from August 20 to September 10 - to 0.6 g in the favourable period - the first ten days of August. A significant dependence of the mass of 1000 pcs. Based on the average daily air temperature and the sum of active temperatures above 15 °C, the correlation coefficient (r) ranged from 0.976 to 0.978. The conducted studies have revealed several features of the formation of the crop structure of a new variety of Clarkia, depending on topographic, matural, and ecological variability. The actual seed productivity of the clarkia variety Raspberry bowl in the south of Western Siberia is, on average, 50% of the potential. From one varietal plant in the south of Western Siberia, 3.026 g of seeds are obtained (on average, over three years of research). The established relationships between the quality of seed material and the main climatic factors make it possible to control and, to a certain extent, predict the seed yield of the new variety Clarkia amoena Raspberry bowl in the sharply continental climatic conditions of the south of Western Siberia.
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Koroleva, E. V., A. F. Petrov und Yu V. Chudinova. „Intraspecific variability of floral and ornamental plants of Onagraceae family and mechanisms of their variety formation“. Bulletin of NSAU (Novosibirsk State Agrarian University), Nr. 2 (13.07.2021): 18–42. http://dx.doi.org/10.31677/2072-6724-2021-59-2-18-42.

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The present review is devoted to intraspecific variability of floral and ornamental plants of the genus Clarkia purch. Family Onagraceae and clarification of the systematic status of the Godetia species introduced into Novosibirsk. Clarkia species from the Godetia and Rhodantos sections are noted for their particular beauty and duration of flowering. A comprehensive study of the influence of environmental conditions on genetic features of populations, ornamental qualities, flowering biology, morphogenesis, organogenesis, seed production and seed quality, agrotechnology’s of growing the above species of Clarkia, for selecting suitable forms and creating local varieties included in the basic assortment of flower crops for climatic conditions of Western Siberia as well as their introduction into urban gardening is necessary. The article discusses taxonomic markers important for determining the systematic position of Clarkia species from Godetia and Rhodantos sections in the family Onagraceae. Experimental data of genetic and cytological analyses carried out by foreign scientists for the last 100 years are presented, which give an idea of morphological, karyological characteristics and genetic variability in various species in the Godetia section. The mechanisms of inheritance of the flower colour trait and numerous variations in the position of the spot on the petals of different clarkia species have been established. Formation of the list of genes responsible for the Clarkia pigmentation system, location and size of the carmine spot will allow targeted selection for qualitative traits. The study of the pattern of multifactorial inheritance of habitus and flower shape traits among C. amoena subspecies contributes to the passage of the most decorative hybrid forms (varieties) as the starting material. In conclusion, a brief overview of promising species of the genus Clarkia from the Godetia and Rhodantos sections for ornamental plant breeding and selection is given.
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Koroleva, E. V. „Variability of reproductive seed quality in <i>Сlarkia amoena</i> (Lehm.) A. Nelson & J.F. Macbr)) depending on storage duration under ex-situ conditions“. Bulletin of NSAU (Novosibirsk State Agrarian University), Nr. 3 (09.10.2023): 54–65. http://dx.doi.org/10.31677/2072-6724-2023-68-3-54-65.

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This paper presents the results of assessing the reproductive seed quality of Clarkia amoena (Lehm) A. Nelson & J.F. Macbr) seeds during storage under laboratory conditions (temperature 20–22°C). The seeds were stored in paper bags for eight years. The authors selected seed samples from pods of varying degrees of maturity (open and closed) and different positions on the mother plant (branches of other orders). The authors set up three groups based on their location on the mother plant: 1) Sixty-four seed samples were formed on the main stem (firstorder branches); 2) Sixty-four seed samples were created on second-order branches; 3) Sixty-four seed samples were created on third-order branches and two groups based on fruit maturity: open and closed pods. In total, 192 seed samples were evaluated. Seed quality assessment was conducted annually in April, following GOST 24933.081 standards for flower crop seeds. The initial laboratory germination of seeds ranged from 69% for samples taken from closed pods on third-order branches to 97% for samples taken from open pods on the main stem. It decreased with an increase in the seed storage period according to a linear regression equation: Y = 7.673–0.064X, where Y is the storage duration, and X is the seed germination rate. Over eight years of storage, the 1000-seed mass decreased by 37.2% for samples from the main stem's open pods and 30.3% for samples from closed pods, by 43.6% and 46.8% for second-order branches, and by 46.1% and 50.9% for third-order extensions, respectively. On average, the 1000-seed mass decreased by 0.2 g for all samples over eight years of storage. Regression and correlation analyses (Pearson's coefficient) revealed significant negative relationships between storage duration and seed germination energy (r = –0.849), germination rate (r = –0.853), and 1000-seed mass (r = –0.790). A strong positive correlation was observed between seed mass and viability (r = 0.886). Over eight years of storage, seeds from the main stem lost their germination rate to 18–23%, significantly lower than the initial rate. In contrast, second-order branch seeds decreased by half, and roots from third-order branches completely lost viability. A multidimensional model of the interaction of factors—storage duration and branch order—on seed viability under ex-situ conditions was constructed, demonstrating that the overall viability of Clarkia amoena seeds is maintained for 4–5 years.
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Dar, Riyaz Ahmad, Shaziya Nisar und Inayatullah Tahir. „Alleviation of Antioxidant Enzyme Activity by an Hour 0.25 mM Silver Thiosulphate Pulse Duration in Clarkia amoena“. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 90, Nr. 1 (11.02.2019): 47–53. http://dx.doi.org/10.1007/s40011-019-01079-9.

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Anderson, Robert G., und Wenwei Jia. „EFFECT OF SUPPLEMENTAL AND PHOTOPERIODIC LIGHTING ON FLOWERING OF SATIN FLOWER“. HortScience 28, Nr. 5 (Mai 1993): 520c—520. http://dx.doi.org/10.21273/hortsci.28.5.520c.

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Satin flower (Clarkia amoena ssp. whitneyi; syn. Godetia whitneyi) is a cool temperature plant grown as a cutflower in Japan, Europe and California with great market potential for Christmas and Valentine's Day markets. Sequential crops of 4-week-old plugs of `Grace Rose Pink,'`Grace Salmon,' `Grace Red' and `Satin White' were planted 24 Sept, 15 Oct and 5 Nov 1993. Plants were placed immediately in the following supplemental and photoperiodic lighting treatments: ambient light; incandescent light 1800 to 2400 HR, 8 μmol s-1m-2 HPS; HID light 1800 to 2400 HR, 40 μmol s-1m-2 HPS; HID light 1800 to 2400 HR, 150 μmol s-1m-2 HPS. The node number and days to flower for satin flower plants in the ambient light treatment were significantly different from lighting treatments in each crop. Node number and days to flower were similar in the three lighting treatments for the first crop. Node number was similar for the lighting treatments for the second and third crop but days to flower was significantly different between the lighting treatments.
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Utami, Liliek, Robert G. Anderson, Robert L. Geneve und Sharon Kester. „EFFECT OF SUPPLEMENTAL AND PHOTOPERIODIC LIGHTING ON FLOWERING OF SATIN FLOWER“. HortScience 25, Nr. 9 (September 1990): 1090c—1090. http://dx.doi.org/10.21273/hortsci.25.9.1090c.

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Satin flower (Clarkia amoena ssp. whineyi: syn. Godetia whitneyi) is a cool temperature, high light plant grown as a cutflower in Japan, Europe and California. In preliminary greenhouse cutflower and pot plant trials, satin flower plants flowered in 10-11 weeks when grown under 24hr supplemental HID lighting compared to 20-22 weeks under ambient winter conditions. In Sept. and Nov. 1989, satin flower plants were treated with the following supplemental and photoperiodic lighting treatments ambient light; SD (ambient day, black cloth 1600 to 800 HR); LD (ambient day, incandescent light 1600 to 400 HR, 5 μmol s-1 m-2); SD-SPL (ambient day supplemented with 100 μmol s-1m-2 HPS, black cloth 1600 to 800 HR); LD-SPL (ambient day supplemented with 100 μmol s-1m-2 800 to 400 HR), Node number and days to flowering were significantly different between the treatments. Plants grown under LD-SPL flowered in 10 weeks and had 32 nodes, LD -13 weeks and 37 nodes (weak, spindly, few flowers), SD-SPL - 17 weeks and 70 nodes, SD - 21 weeks and 75 nodes. Strategies for supplemental lighting for greenhouse cutflower production will be discussed.
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Utami, Liliek S., Robert L. Geneve, Robert G. Anderson und Sharon T. Kester. „THE EFFECTS OF PINCHING, SPACING, AND PLANTING DATE ON CUTFLOWER PERFORMANCE OF SATINFLOWER.“ HortScience 25, Nr. 9 (September 1990): 1090f—1090. http://dx.doi.org/10.21273/hortsci.25.9.1090f.

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Satin flower (Clarkia amoena ssp. whitneyi - syn. Godetia) is a cool season native to the Western U.S. being studied for its potential use as a cutflower crop in Kentucky. In May 1989, plants of `Grace Salmon' were transplanted to the field into black fabric mulch. A factorial experiment was conducted with three pinching treatment (no pinch, pinched early at the third mode prior to transplanting, and pinched in the field at the third mode after the first flower bud was visible) and at three spacings (15, 30 end 45 cm). There were significant main and interaction effects for both pinching and spacing for the number of flowering stems, stem length and plant diameter. However, no treatment combination consistently produced flowering stems of sufficient length for commercial quality. This may be due to the later spring planting date and hot weather in 1989. In an attempt to increase flower stem length, Godetia `Grace Salmon' plants have bean transplanted on April 10, 25 and May 10, Plants will be pinched in the greenhouse or grown without pinching et 45 or 15 cm spacing, respectively, The. effect of supplemental lighting and long days during transplant production will also be considered,
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Hartley, Gwendolyn, und Robert G. Anderson. „PRODUCTION OF SATIN FLOWER POTTED PLANTS FROM CUTTINGS“. HortScience 25, Nr. 8 (August 1990): 865a—865. http://dx.doi.org/10.21273/hortsci.25.8.865a.

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Satin flower (Clarkia amoena subsp. whitneyi; syn. Godetia whitneyi) is a cool temperature, high light plant used as a cutflower in Japan, Europe and California. The stiking flower color patterns, large, long-lasting flowers and branching pattern could make this plant an important potted flowering plant. Cuttings, 6-8 cm long with flower buds (0.5-2.0 cm long), were harvested from secondary and tertiary stems of field-grown pinched plants of `Grace Red'. Cuttings were rooted in intermittent mist and potted in 10 cm pots two weeks later. The terminal flower buds and stem tip were removed one week later and the plants flowered within 4 weeks with 4-6 equal lateral branches. In the fall, `Grace Rose Pink' seed was sown Oct. 4, 1989, plants were grown under 100 umole s-1 cm-2 HPS 18 hr. supplemental light. Cuttings from the primary stem were harvested at the same stage, rooted in two weeks, potted and pinched one week later. Cuttings grown under supplemental light flowered 4 weeks later while cuttings grown under ambient light flowered over 10 weeks later. Over 70% of the terminal cuttings in supplemental light were 22-30 cm tall with 4-6 equal lateral branches that flowered uniformly.
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Mahmoudi, Mohammad Reza, Nozhat Zebardast, Frederick R. Masangkay und Panagiotis Karanis. „Detection of potentially pathogenic free-living amoebae from the Caspian Sea and hospital ward dust of teaching hospitals in Guilan, Iran“. Journal of Water and Health 19, Nr. 2 (22.02.2021): 278–87. http://dx.doi.org/10.2166/wh.2021.240.

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Abstract Free-living amoebae (FLA) thrive in diverse environmental conditions. The present study aimed to define the FLA distribution from the Caspian Sea as well as from hospital ward dust from Guilan, Iran. Seawater (20) and hospital ward dust samples (100) were collected from May to June 2018. Seawater samples were vacuum filtered through a 0.45 μm pore-size membrane. Dust was collected using sterile gauze, washed with sterile distilled water, with washings collected thereafter. Washings were similarly filtered as seawater samples. FLA from the filtered material was cultivated in non-nutrient agar. Molecular analysis was performed by PCR and sequencing using specific primers for Acanthamoeba, Naegleria, and Vermamoeba/Hartmanella. Culture and PCR returned 50 and 65% positivity, respectively, for seawater samples where sequencing revealed Acanthamoeba T2, T5 and T6 genotypes and A. palestinensis and A. lenticulata, as well as N. dobsoni and N. clarki. In addition, 30% amoebic growth and 16% PCR detection were observed from hospital ward dust samples where sequencing revealed Acanthamoeba T2, T4 and T11 genotypes and A. castellanii, A. palestinensis and A. stevensoni as well as N. clarki. For both seawater and dust samples, Acanthamoeba was the dominant isolate. The detection of potentially pathogenic FLA from seawater may pose a threat to the public, while the presence of the same in dust spells threats to both hospital staff and patients, in particular, immunocompromised individuals. Public education, awareness, improved sanitation and hygiene, and the crafting of diagnostic strategies for the early detection of FLA in humans are necessary for the mitigation and management of potential human infection cases.
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Spall, Caleb E., und Roberto G. Lopez. „Supplemental Lighting Quality Influences Time to Flower and Finished Quality of Three Long-Day Specialty Cut Flowers“. Horticulturae 9, Nr. 1 (06.01.2023): 73. http://dx.doi.org/10.3390/horticulturae9010073.

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Year-round demand for locally sourced specialty cut flowers continues to increase. However, due to low radiation intensities and temperatures, growers in northern latitudes must utilize greenhouses, but limited production information detailing manipulation of the radiation environment exists. Therefore, our objective is to quantify the influence of supplemental lighting (SL) quality on time to flower and harvest and stem quality of three long-day specialty cut flowers. Godetia ‘Grace Rose Pink’ (Clarkia amoena), snapdragon ‘Potomac Royal’ (Antirrhinum majus), and stock ‘Iron Rose’ (Matthiola incana) plugs are transplanted into bulb crates and placed in one of six greenhouse compartments with SL providing a total photon flux density of 120 µmol·m−2·s−1 from 0700 to 1900 HR. After four weeks, SL is extended to provide a 16 h photoperiod to induce flowering. SL treatments are provided by either high-pressure sodium (HPS) fixtures or various light-emitting diode (LED) fixtures. Treatments are defined by their 100 nm wavebands of blue (B; 400–500 nm), green (G; 500–600 nm), red (R; 600–700 nm), and far-red (FR; 700–800 nm) radiation (photon flux density in μmol·m−2·s−1) as B7G60R44FR9 (HPS120), B20G50R45FR5, B20R85FR15, B30G25R65, B120, or R120. Time to harvest (TTH) is up to 14, 15, and 10 d slower under R120 SL for godetia, snapdragon, and stock, respectively, compared to the quickest treatments (HPS120, B120, and B20R85FR15 SL). However, R120 SL produces cut flowers up to 18% longer than those grown under the quickest treatments. Both broad-spectrum LED fixtures slightly delay TTH compared to the quickest treatments. Stem caliper is not commercially different between treatments for godetia or snapdragon, although stems are up to 14% thinner for stock grown under B120 SL compared to the other treatments. Flower petal color is not commercially different between SL treatments. We recommend utilizing a SL fixture providing a spectrum similar to B20R85FR15 SL or B20G50R45FR5, as they elicit desirable crop responses with minimal developmental, quality, and visibility tradeoffs. While HPS lamps perform similarly to the recommended fixtures, we recommend utilizing LEDs for their higher photon efficacy and potential energy savings.
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„Clarkia amoena“. CABI Compendium CABI Compendium (07.01.2022). http://dx.doi.org/10.1079/cabicompendium.113347.

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Lin, Rong-Chien, und Mark D. Rausher. „Ancient Gene Duplications, Rather Than Polyploidization, Facilitate Diversification of Petal Pigmentation Patterns in Clarkia gracilis (Onagraceae)“. Molecular Biology and Evolution, 16.08.2021. http://dx.doi.org/10.1093/molbev/msab242.

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Abstract It has been suggested that gene duplication and polyploidization create opportunities for the evolution of novel characters. However, the connections between the effects of polyploidization and morphological novelties have rarely been examined. In this study, we investigated whether petal pigmentation patterning in an allotetraploid Clarkia gracilis has evolved as a result of polyploidization. Clarkia gracilis is thought to be derived through a recent polyploidization event with two diploid species, C. amoena huntiana and an extinct species that is closely related to C. lassenensis. We reconstructed phylogenetic relationships of the R2R3-MYBs (the regulators of petal pigmentation) from two subspecies of C. gracilis and the two purported progenitors, C. a. huntiana and C. lassenensis. The gene tree reveals that these R2R3-MYB genes have arisen through duplications that occurred before the divergence of the two progenitor species, that is, before polyploidization. After polyploidization and subsequent gene loss, only one of the two orthologous copies inherited from the progenitors was retained in the polyploid, turning it to diploid inheritance. We examined evolutionary changes in these R2R3-MYBs and in their expression, which reveals that the changes affecting patterning (including expression domain contraction, loss-of-function mutation, cis-regulatory mutation) occurred after polyploidization within the C. gracilis lineages. Our results thus suggest that polyploidization itself is not necessary in producing novel petal color patterns. By contrast, duplications of R2R3-MYB genes in the common ancestor of the two progenitors have apparently facilitated diversification of petal pigmentation patterns.
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Przemysław Bąbelewski und Magdalena Pancerz. „Autumn Cultivation of Farewell-To-Spring (Clarkia amoena A. Nelson & J. F. Macbr.) in Unheated Foil Tunnel in Lower Silesia Condition“. Journal of Agricultural Science and Technology A 5, Nr. 5 (28.05.2015). http://dx.doi.org/10.17265/2161-6256/2015.05.005.

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Rovere, Adriana. „Los canteros urbanos como parte del paisaje biocultural de Bariloche (Argentina): riqueza de especies y decisiones de manejo“. Boletín de la Sociedad Argentina de Botánica 57, Nr. 3 (30.09.2022). http://dx.doi.org/10.31055/1851.2372.v57.n3.37477.

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Introducción: En áreas urbanas, los canteros con especies ornamentales, brindan estructura y color, atraen polinizadores, funcionan como corredores ecológicos, acercan la naturaleza, y ofrecen bienestar a los seres humanos. La flora urbana, puede ser reservorio de biodiversidad, o fuente de invasiones según los criterios de selección. Objetivos: Se estimó la riqueza de especies, formas de vida, familias botánicas y origen de las plantas utilizadas en canteros de Bariloche. M&M: La ciudad de Bariloche, se halla inmersa en el Parque Nacional Nahuel Huapi, posee características pluriculturales. Se estudiaron 1120 canteros ubicados sobre las veredas, con distinto manejo, 560 de gestión municipal y 560 vecinal. Resultados: La riqueza fue de 121 especies, siendo las más utilizadas Lobelia erinus (23,3%) y Clarkia amoena (19,7%). De las 54 familias botánicas representadas, las de mayor frecuencia de uso fueron Rosaceae (28,5%, repartida en 9 especies), Campanulaceae (23,7%, 2) y Compositae (20,4%, 18). Predominó el uso de plantas herbáceas (70 especies), seguidas por arbustos (43), renovales arbóreos (6), y enredaderas (2). Los vecinos manejan mayor diversidad de plantas y especies nativas (92 especies/11 nativas), que el municipio (64/4). La presencia de elementos nativos (e.g., Alstroemeria aurea y Fucsia magellanica) tiene implicancias para la conservación; mientras que las especies invasoras representan riesgo (e.g., Hedera helix y Cytisus scoparius) y deberían ser controladas. Conclusiones: Se concluye que la flora de los canteros representa parte del paisaje biocultural de la región, como escenarios dinámicos construidos a lo largo del tiempo, presentando similitudes con los patrones de selección a nivel mundial.
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