Thematische Bibliographien / CAV-3 gene

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  1. Zeitschriftenartikel
  2. Dissertationen
  3. Bücher
  4. Buchteile
  5. Konferenzberichte
  6. Berichte der Organisationen

Auswahl der wissenschaftlichen Literatur zum Thema „CAV-3 gene“

Autor: Grafiati
Veröffentlicht am 25. Mai 2024

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Zeitschriftenartikel zum Thema "CAV-3 gene"

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Yu, Wan-cheng, Hai-ying Chen, Hong-li Yang та ін. "rBMSC/Cav-1F92A Mediates Oxidative Stress in PAH Rat by Regulating SelW/14-3-3η and CA1/Kininogen Signal Transduction". Stem Cells International 2019 (28 жовтня 2019): 1–11. http://dx.doi.org/10.1155/2019/6768571.

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Background/Objectives. Carbonic anhydrase 1 (CA1)/kininogen and selenoprotein W (SelW)/14-3-3η signal transduction orchestrate oxidative stress, which can also be regulated by nitric oxide (NO). The mutated caveolin-1 (Cav-1F92A) gene may enhance NO production. This study explored the effect of Cav-1F92A-modified rat bone marrow mesenchymal stem cells (rBMSC/Cav-1F92A) on oxidative stress regulation through CA1/kininogen and SelW/14-3-3η signal transduction in a rat model of monocrotaline- (MCT-) induced pulmonary arterial hypertension (PAH). Method. PAH was induced in rats through the subcuta
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Merlini, L. "Familial isolated hyperCKaemia associated with a new mutation in the caveolin-3 (CAV-3) gene." Journal of Neurology, Neurosurgery & Psychiatry 73, no. 1 (2002): 65–67. http://dx.doi.org/10.1136/jnnp.73.1.65.

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Tekin, Hande, and Pınar Edem. "CAV-3-related age-dependent muscle diseases: A novel mutation in mother and son." Neurology Asia 28, no. 3 (2023): 751–55. http://dx.doi.org/10.54029/2023scy.

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The caveolin-3 protein encoded by the CAV-3 gene is a muscle-specific protein found in skeletal, smooth, and cardiac muscle. Caveolin-3 defects lead to several muscle diseases: rippling muscle disease (RMD), limb-girdle muscular dystrophy (LGMD1C), distal myopathy, familial hypertrophic cardiomyopathy, and asymptomatic hyper-CK-emia. While some variants that cause mutations in this gene cause a pure type of disease, some variants may appear as overlap syndromes. Even in the same variants of CAV-3 mutation, the type of muscle disease, its severity, and time of occurrence can be variable. For th
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Kalinowski, M., Ł. Adaszek, P. Miłoszowska, et al. "Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough." Polish Journal of Veterinary Sciences 15, no. 3 (2012): 425–30. http://dx.doi.org/10.2478/v10181-012-0066-7.

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AbstractThe aim of this study was to perform molecular analysis of canine adenovirus 2 (CAV-2) E1B 19K gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the Lubelszczyzna region. Nasal swabs were taken from dogs. DNA of CAV-2 was detected using the PCR method in 16 swabs. All PCR products were sequenced, and the obtained sequences were compared with each other and with the sequence of the E1B 19K gene of the CAV-2 strain from an online database of NCBI GenBank: AC 000003. Based on an
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Szelechowski, Marion, Annie Fournier, Jennifer Richardson, Marc Eloit, and Bernard Klonjkowski. "Functional organization of the major late transcriptional unit of canine adenovirus type 2." Journal of General Virology 90, no. 5 (2009): 1215–23. http://dx.doi.org/10.1099/vir.0.007773-0.

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Vectors derived from canine adenovirus type 2 (CAV-2) are attractive candidates for gene therapy and live recombinant vaccines. CAV-2 vectors described thus far have been generated by modifying the virus genome, most notably early regions 1 and 3 or the fiber gene. Modification of these genes was underpinned by previous descriptions of their mRNA and protein-coding sequences. Similarly, the construction of new CAV-2 vectors bearing changes in other genomic regions, in particular many of those expressed late in the viral cycle, will require prior characterization of the corresponding transcript
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He, Miaomiao, Jie Qiu, Yan Wang, Yang Bai, and Guangzhi Chen. "Caveolin-3 and Arrhythmias: Insights into the Molecular Mechanisms." Journal of Clinical Medicine 11, no. 6 (2022): 1595. http://dx.doi.org/10.3390/jcm11061595.

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Caveolin-3 is a muscle-specific protein on the membrane of myocytes correlated with a variety of cardiovascular diseases. It is now clear that the caveolin-3 plays a critical role in the cardiovascular system and a significant role in cardiac protective signaling. Mutations in the gene encoding caveolin-3 cause a broad spectrum of clinical phenotypes, ranging from persistent elevations in the serum levels of creatine kinase in asymptomatic humans to cardiomyopathy. The influence of Caveolin-3(CAV-3) mutations on current density parallels the effect on channel trafficking. For example, mutation
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Pallister, Jackie, Kevin J. Fahey, and Michael Sheppard. "Cloning and sequencing of the chicken anaemia virus (CAV) ORF-3 gene, and the development of an ELISA for the detection of serum antibody to CAV." Veterinary Microbiology 39, no. 1-2 (1994): 167–78. http://dx.doi.org/10.1016/0378-1135(94)90097-3.

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Robinson, J. M. "What Can Epitope Specific Antibodies Tell us About the Organization of Caveolin in Cells?" Microscopy and Microanalysis 7, S2 (2001): 1030–31. http://dx.doi.org/10.1017/s1431927600031226.

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There are three members of the caveolin (CAV) gene family that give rise to four polypeptides. These polypeptides are CAV-1α, CAV-1β, CAV-2, and CAV-3. The CAV-1β isoform is a truncated form of CAV-1α that lacks 31 amino acids at the N-terminus of the molecule. The CAV- 1β molecule arises through an alternative splicing mechanism.Caveolae are specialized plasma membrane microdomains that are expressed at high levels in some cell types (e.g., endothelium, adipocytes, fibroblasts). These specialized regions of the plasma membrane have a characteristic omega-shaped appearance with diameters rangi
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Smuts, Heidi E. M. "Novel Gyroviruses, including Chicken Anaemia Virus, in Clinical and Chicken Samples from South Africa." Advances in Virology 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/321284.

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Introduction. Chicken anaemia virus, CAV, was until recently the only member of theGyrovirusgenus. 6 novel gyroviruses, AGV2, HGyV1, and GyV3-6, have since been discovered in human and chicken samples.Methods. PCR amplification of the VP2 gene was used to detect AGV2/HGyV1, GyV3, and CAV in a range of clinical samples including stool, respiratory, CSF, and HIV-positive plasma. Screening of fresh local chicken meat was also performed.Results. AGV2/HGyV1 or GyV3 was detected in stools from healthy children (17/49, 34.7%) and patients with diarrhoea (22/149, 14.8%). 1.2% (3/246) nasopharyngeal re
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Nogueira, E. O., L. Brentano, and A. J. P. Ferreira. "A VP3/VP1 gene polymerase chain reaction assay for detection of chicken anemia virus in broiler samples." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 57, suppl 2 (2005): 131–40. http://dx.doi.org/10.1590/s0102-09352005000800001.

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A PCR assay was designed for amplification of the highly conserved VP3 gene and a 5' region of the VP1 gene, for the diagnosis of CAV in organ samples of broiler flocks suspected of chicken infectious anemia. A comparison of the VP3/VP1 PCR with in vivo virus isolation revealed 100% agreement of the results, with 13 positive and 3 negative samples in both assays, indicating that the VP3/VP1 PCR is a specific diagnostic method. Tissues from additional 24 broiler chicken flocks, with CAV-like lesions and clinical history were then tested only by the VP3/VP1 PCR and a reference PCR with published
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Dissertationen zum Thema "CAV-3 gene"

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Lemerle, Eline. "Rôle des cavéoles dans la formation des tubules-T et dans la physiopathologie des cavéolinopathies." Electronic Thesis or Diss., Sorbonne université, 2021. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2021SORUS010.pdf.

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Dans le muscle squelettique, des invaginations du sarcolemme appelées cavéoles et leur composant principal Cav-3 seraient impliqués dans la formation des tubules transverses, des structures musculaires permettant de propager le potentiel d'action dans la fibre musculaire. Pourtant, ce mécanisme demeure à ce jour inconnu. L’importance des cavéoles et de Cav-3 est accentuée par l’existence de défauts dans l’organisation et la fonction des cavéoles dans le cas de cavéolinopathies, des maladies neuromusculaires autosomiques dominantes dues à des mutations dans le gène CAV-3 et dont les mécanismes
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Song, Xiaomin. "Distribution and molecular characterization of 3'-UTR binding proteins specific to the (U)¦1¦5 region of Cas-1 gene." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0013/MQ28664.pdf.

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Stanojevic, Nadia. "The yeast cap-binding complex is co-transcriptionally recruited to the ADH1 gene and is not required for recruitment of the elongation, RNA 3'-end formation and export machineries in vivo /." Available to subscribers only, 2005. http://proquest.umi.com/pqdweb?did=1079658481&sid=3&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Kiss, Andor Joseph. "A spatial and temporal study of mRNA binding proteins specific to a 134 base CA dinucleotide-rich portion of the 3'-untranslated region from the BALB/c mouse catalase gene, Cas-1." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq28596.pdf.

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Novak, Gabriela. "Upregulation of CaMKIIβ and Nogo-C mRNA in Schizophrenia and the Prevalence of CAA Insert in the 3’UTR of the Nogo Gene". Thesis, 2008. http://hdl.handle.net/1807/11240.

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Schizophrenia may result from altered gene expression leading to abnormal neurodevelopment. In a search for genes with altered expression in schizophrenia, cDNA library subtractive hybridization experiments using post-mortem human frontal cerebral cortices from schizophrenia individuals and neurological controls were performed. I found the mRNA of two neurodevelopmentally important genes, Nogo (RTN4) and calcium/calmodulin-dependent protein kinase II beta (CaMKIIβ), to be overexpressed in post-mortem frontal cortex tissues from patients who suffered with schizophrenia. I used the quantitative
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Rodriguez, Angel E. "Characterization of CAL 27 and HSC-3 cell lines. DPAGT1 gene expression and association with oral squamous cell carcinoma genesis and metastasis." Thesis, 2016. https://hdl.handle.net/2144/18669.

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Cancer, a disease of an uncontrolled cell division, growth and metastasis as a result of genetic mutations, environmental factors and host response, is affecting populations worldwide. Etiology, pathogenicity, and genetics related to cancer are not well understood, and treatment has not been as effective as scientists have expected. Continual research is being done to improve current understanding and treatments. Oral squamous cell carcinoma (OSCC) is one of the most common head and neck cancers (representing >90 % of all head and neck cancers) involving neoplasms of the oral cavity and or
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Kellner, Tobias [Verfasser]. "Expressionsanalyse der potenziellen Tumor-Suppressor-Gene H-REV107-2/TIG 3 & MUC18/Mel-CAM in humanen Ovarialkarzinom-Zelllinien und Ovarialkarzinomen / von Tobias Kellner." 2007. http://d-nb.info/988265400/34.

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Bücher zum Thema "CAV-3 gene"

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Joyner, Alexandra, ed. Gene Targeting. Oxford University Press, 1999. http://dx.doi.org/10.1093/oso/9780199637928.001.0001.

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Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first
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Wade, Tracey D., and Cynthia Bulik. Genetic Influences on Eating Disorders. Edited by W. Stewart Agras and Athena Robinson. Oxford University Press, 2017. http://dx.doi.org/10.1093/oxfordhb/9780190620998.013.5.

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The current chapter reviews our progress in understanding how genes influence eating disorders by addressing the following areas: (1) how recognition of genetic influences on eating disorders emerged; (2) the complexities of gene environment interplay; (3) what twin studies can tell us about gene environment interplay, and (4) the current state of molecular genetic studies. It is concluded that both genes and nonshared environment play a critical role in the explanatory framework for the etiology of eating disorders. Shared environment is likely to contribute to the development of cognition an
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Dode, L. Identification and Characterisation of the Human Sarco/ Endoplasmic Reticulum Ca2+-Atpase 3 (Serca3) Gene. Leuven University Press, 1998.

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Schrag, Brian, and Kathleen J. Van Buren. Connect Goals to Genres. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780190878276.003.0004.

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Step 3 follows Step 2, in which community members choose a goal for artistic programs. Step 3 provides guidance for choosing the desired effects of arts programs; choosing the content of arts programs; choosing a genre or genres that can communicate the content and produce the desired effects; and imagining events that could include the performance of new artistic works. Specific suggestions and tables (such as the Genre Comparison Chart) assist readers in moving through these processes. This step also considers how communication occurs through an artistic event. Step 3 concludes with a note o
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Maj, Dorota. Modyfikujący wpływ roślinnych dodatków paszowych na użytkowość mięsną i ekspresję wybranych genów u królików w zależności od wieku i płci. Publishing House of the University of Agriculture in Krakow, 2017. http://dx.doi.org/10.15576/978-83-66602-29-8.

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The aim of the study was to determine the effect of feed additives (algae, soybean, and sunflower oil) used in the rabbit feed on: growth indices and slaughter traits, pH, colour, texture, chemical composition, fatty acid profile and oxidative stability (TBARS) of the meat as well as FTO and FABP4 genes expression in the meat’s intramuscular fat (m. longissimus lumborum), depending on the age and sex. The experimental material consisted of Termond White rabbits (n = 160, 80 females and 80 males). Animals were weaned on the 35th day of life, and housed in metal cages arranged in batteries (4 ra
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Kirchman, David L. Predation and protists. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198789406.003.0009.

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Protists are involved in many ecological roles in natural environments, including primary production, herbivory and carnivory, and parasitism. Microbial ecologists have been interested in these single-cell eukaryotes since Antonie van Leeuwenhoek saw them in his stool and scum from his teeth. This chapter focuses on the role of protozoa (purely heterotrophic protists) and other protists in grazing on other microbes. Heterotrophic nanoflagellates, 3–5 microns long, are the most important grazers of bacteria and small phytoplankton in aquatic environments. In soils, flagellates are also importan
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Laland, Kevin N., and Gillian R. Brown. The Social Construction of Human Nature. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198823650.003.0008.

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What is the job that the term ‘human nature’ is expected to do? Three notions are prevalent but are problematic: (1) Distinguishing what is biological from what is cultural/environmental. Here the term fails. (2) Characterizing the defining features of humanity, thereby allowing us to be distinguished from other species. This stance is tenable but contributes little. (3) Characterizing what is universal or typical about humanity, because of our ‘evolved biological heritage’. Here the term is tenable but misleading and hence counterproductive. ‘Human nature’ is equally reciprocally caused by ge
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Muir, Stephanie. Studying City of God. Liverpool University Press, 2008. http://dx.doi.org/10.3828/liverpool/9781903663592.001.0001.

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A leading example of a resurgent Latin American cinema — ‘la buena onda’ — in the early twenty-first century, City of God was a huge international popular and critical success. A combination of intoxicating, Hollywood-style genre film-making and hard-hitting, social-realist subject matter, it was hailed as a masterpiece at Cannes in 2002 and seen by over 3 million people in Brazil, including the Brazilian cabinet. This book considers: The historical and industrial context of City of God — a brief history of Latin American cinema is followed by a more detailed account of film-making in Brazil —
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Harding, Sian E. The Exquisite Machine. The MIT Press, 2022. http://dx.doi.org/10.7551/mitpress/12836.001.0001.

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How science is opening up the mysteries of the heart, revealing the poetry in motion within the machine. Your heart is a miracle in motion, a marvel of construction unsurpassed by any human-made creation. It beats 100,000 times every day—if you were to live to 100, that would be more than 3 billion beats across your lifespan. Despite decades of effort in labs all over the world, we have not yet been able to replicate the heart's perfect engineering. But, as Sian Harding shows us in The Exquisite Machine, new scientific developments are opening up the mysteries of the heart. And this explosion
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Branham, R. Bracht. Inventing the Novel. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198841265.001.0001.

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Bakhtin as a philosopher and a student of the novel is intent upon the novel’s role in the history of consciousness. His project fails if he is wrong about the dialogic nature of consciousness or the cultural centrality of the novel as the only discourse that can model human consciousness and its intersubjective character. Inventing the Novel is an argument in four stages: the Introduction surveys Bakhtin’s life and his theoretical work in the 1920s, which grounded his work on the novel, as investigated in following chapters. Chapter 1 sketches Bakhtin’s view of literary history as an agonisti
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Buchteile zum Thema "CAV-3 gene"

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Ross, Samuel E., and Ozren Bogdanovic. "Generation and Molecular Characterization of Transient tet1/2/3 Knockouts." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1294-1_17.

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Abstract5-methylcytosine (5mC) is a gene-regulatory mark associated with transcriptional repression. 5mC can be erased through the catalytic action of Ten-eleven translocation (TET) methylcytosine dioxygenases (TET1, TET2, TET3), which oxidize 5mC resulting in its removal from the genome. In vertebrates, TET enzymes facilitate DNA demethylation of regulatory regions linked to genes involved in developmental processes. Consequently, TET ablation leads to severe morphological defects and developmental arrest. Here we describe a system that can facilitate the study of relationships between TET enzymes, 5mC, and embryo development. We provide detailed descriptions for the generation of F0 zebrafish tet1/2/3 knockouts using CRISPR/Cas9 technology and elaborate on the strategies to assess the impact of TET loss by reduced representation bisulfite sequencing (RRBS).
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Zhang, Lei, and Jian-Jun Hu. "Transgenic poplar gene flow monitoring in China." In Gene flow: monitoring, modeling and mitigation. CABI, 2021. http://dx.doi.org/10.1079/9781789247480.0004.

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Abstract Poplar is cultivated widely for pulpwood, firewood, and timber. Transgenic poplar may be part of a solution for wood demand in China. Because transgene escape is an important part of ecological security evaluation of transgenic plants, in this chapter we discuss a real transgenic poplar case study. In this case study, mature transgenic male Populus nigra plants harbored a Bacillus thuringiensis toxin gene (i.e. Bt poplar). A plantation of these plants served as a testbed for a relevant example for gene flow monitoring in China. Furthermore, we discuss environmental risk assessment (ERA) of these transgenic plants. While transgenes can drift to related species through natural and controlled pollination, the probability of transgene drift appears to be very low in the field. The resultantBt poplar seeds occurred at a frequency from about 0.15% at 0 m to about 0.02% at 500 m away from the Bt poplar. The Bt poplar progeny seeds had decreased germination within 3 weeks in the field (from 68% to 0%), compared with the 48% germination rate after 3 weeks at 4°C. The survival rate of seedlings in the field was 0% without any treatments, but increased to 1.7% under four combined treatments (clean and trim, watering, weeding, and cover with plastic to retain moisture) after being seeded in the field for 8 weeks. Hybrid offspring appeared to possess segregated traits following artificially controlled pollination. While hybrids of transgenic poplar and non-transgenic poplar can be excellent germplasm, gene flow should be monitored. Transgene expression in grafted scion and rootstock of transgenic poplar is reviewed. The transgenic poplar studied appears to be safe; no ecological or environmental harm has been observed in China.
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Abe, Tomoko, Hiroyuki Ichida, Yoriko Hayashi, et al. "Ion beam mutagenesis - an innovative and effective method for plant breeding and gene discovery." In Mutation breeding, genetic diversity and crop adaptation to climate change. CABI, 2021. http://dx.doi.org/10.1079/9781789249095.0042.

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Abstract We have developed a unique technology for mutation induction of plants using energetic ion beams at the RI Beam Factory (RIBF) of Rikagaku Kenkyūjo (RIKEN) (Institute of Physical and Chemical Research). Ion beams effectively induce mutations at relatively low doses without severely inhibiting growth. The irradiation treatment can be given to various plant materials and mutation can be induced in a short time, between seconds and a few minutes. The linear energy transfer (LET) of ions depends on the nuclide and velocity. Since LET value affects the mutation frequency, it is an important parameter to determine the most effective irradiation condition in mutagenesis. We determined the most effective dose in each LET for mutation induction in imbibed rice seeds. Subsequently, we analysed the mutated DNA responsible for the phenotype in morphological mutants. Most of the mutations were small deletions of less than 100 bp. Irradiations of C-ions and Ne-ions are effective for plant breeding because of the very high mutation rate and sufficient energy to disrupt a single gene. On the other hand, all mutations induced by Ar-ion (290 keV/μm) irradiation were large deletions ranging from 176 bp to approximately 620 kb. The average number of mutations in the target exon regions was 7.3, 8.5 and 4.3 per M<sub>3</sub> mutant plant in C-ions, Ne-ions and Ar-ions, respectively. The number of mutations induced by heavy-ion irradiation was relatively small. We could identify six responsible genes for eight mutants induced by C-ion and Ne-ion irradiations and two responsible genes for four mutants induced by Ar-ion irradiation. Three of these were genes not previously described.
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Garanto, Alejandro. "Delivery of Antisense Oligonucleotides to the Mouse Retina." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2010-6_22.

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AbstractThe eye is the organ in charge of vision and, given its properties, has become an excellent organ to test genetic therapies, including antisense oligonucleotide (AON) technology. In fact, the first AON receiving FDA and EMA approval was meant to treat an eye condition. Currently, dozens of clinical trials are being conducted for a variety of subtypes of inherited retinal disease. Although most of them are based on gene augmentation therapies, a phase 3 and two phase 1/2 clinical trials using AONs are ongoing. Since the retina is a layered structure of nondividing cells, obtaining human retinal tissue and expanding it in the lab is not possible, unless induced pluripotent stem cell technology is used. Mouse models have helped to elucidate the function of many genes, and the retinal structure is quite similar to that of humans. Thus, drug delivery to the mouse eye can provide valuable information for further optimization of therapies. In this chapter, the protocol for intravitreal injections of AONs is described in detail.
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Paro, Renato, Ueli Grossniklaus, Raffaella Santoro, and Anton Wutz. "Biology of Chromatin." In Introduction to Epigenetics. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-68670-3_1.

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AbstractThis chapter provides an introduction to chromatin. We will examine the organization of the genome into a nucleosomal structure. DNA is wrapped around a globular complex of 8 core histone proteins, two of each histone H2A, H2B, H3, and H4. This nucleosomal arrangement is the context in which information can be established along the sequence of the DNA for regulating different aspects of the chromosome, including transcription, DNA replication and repair processes, recombination, kinetochore function, and telomere function. Posttranslational modifications of histone proteins and modifications of DNA bases underlie chromatin-based epigenetic regulation. Enzymes that catalyze histone modifications are considered writers. Conceptually, erasers remove these modifications, and readers are proteins binding these modifications and can target specific functions. On a larger scale, the 3-dimensional (3D) organization of chromatin in the nucleus also contributes to gene regulation. Whereas chromosomes are condensed during mitosis and segregated during cell division, they occupy discrete volumes called chromosome territories during interphase. Looping or folding of DNA can bring regulatory elements including enhancers close to gene promoters. Recent techniques facilitate understanding of 3D contacts at high resolution. Lastly, chromatin is dynamic and changes in histone occupancy, histone modifications, and accessibility of DNA contribute to epigenetic regulation.
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Maroni, Gustavo. "Size Variations Among the Elements that Constitute the Genes of Drosophila (Leader, coding region 3’ Untranslated Region, Exons, Introns)." In An Atlas of Drosophila Genes. Oxford University PressNew York, NY, 1993. http://dx.doi.org/10.1093/oso/9780195071160.003.0034.

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Abstract The discussion in this chapter centers around two questions: (1) what are the size ranges of the various elements that constitute a functional gene? And (2) is there a correlation between the size of one element and the size of another. The data analyzed in this chapter are derived from 73 of the genes presented in Part I. Because 12 of those 73 genes have multiple transcripts, they encompass a total of 87 transcripts. Two partly overlapping datasets can be examined: Dataset A includes all 87 transcripts, but elements shared by different transcripts of the same gene are considered only once. For example, if two transcripts of a gene differ only with respect to the poly (A) site, both 3’ untranslated regions (3’ UTR) are included in the analysis, but the leader is counted only once, since it is the same for both transcripts. Dataset B includes only one representative from each family of related genes or from the group of multiple transcripts of a given gene. In this case the sample is reduced to 40 “unrelated” transcripts. The size of a few elements were found to be outside the expected size range suggested by statistical analyses and so were excluded from the analysis. These elements are the 3’ UTR ofbsg25D II and the leader, 3’ UTR and introns of Ubx.
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Kuipers, Anja G. J., Evert Jacobsen,, and Richard G. F. Visser. "Applications of Antisense Technology in Plants." In Antisense Technology. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199635832.003.0009.

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Abstract In 1984, Izant and Weintraub (1) published the first experiments on the antisense effect of gene constructs in eukaryotic cells. Since then, the inhibition of gene expression via antisense RNA has developed into a technique that is widely applied in molecular biology. In the field of plant biotechnology and plant molecular biology Ecker and Davis (2) were the first to describe a transient assay system in which carrot protoplasts were simultaneously electro-porated with sense and antisense CAT genes. This resulted in the inhibition of CAT activity of up to 95% as compared to the control which only contained a sense CAT gene. These initial studies were followed by experiments with various plant species, in which the expression of a wide variety of genes has been effectively inhibited, either transiently with in vitro transcribed antisense RNAs, or by the introduction of antisense genes via transformation (reviewed in refs 3-5).
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Reddy, P. Sanjeeva, and Brent H. Cochran. "Novel gene transcription in response to growth factors: gene isolation by differential plaque hybridization." In Growth Factors. Oxford University PressOxford, 1993. http://dx.doi.org/10.1093/oso/9780199633609.003.0004.

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Abstract Differential cDNA cloning can be used to identify differences in gene expression between any two populations of cells. This method has been used in a variety of experimental situations, but has been especially successful in allowing for the isolation of genes which are regulated by growth factors. These screens have identified genes which are regulated by PDGF (1), TPA (2), IL-2 (3), serum (4, 5), and NGF (6) to name only a few. This analysis has increased our knowledge not only about the types of genes which are regulated by different growth factors, but also helped identify entirely new gene families such as the bZIP proteins and novel cytokines (7, 8). These growth factor regulated genes are often referred to as immediate early genes, or early response genes since many of them can be induced by growth factors in the absence of new protein synthesis. For review, see Herschman, 1991 (9).
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Beatty, Barbara G., and Stephen W. Scherer. "Human chromosome mapping of single copy genes." In Fish. Oxford University PressOxford, 2002. http://dx.doi.org/10.1093/oso/9780199638833.003.0003.

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Abstract One of the most common applications of FISH is the localization of single copy genes to a specific chromosomal band on metaphase chromosomes (1-3). Identifying the sub chromosomal position of a gene in both normal and disease states can provide valuable information regarding its biological and/or clinical significance. In some situations, mapping a newly identified gene to regions of chromosomal deletion or amplification, to translocation breakpoints, or to a region near a gene known to be involved in a particular disease process (4-6) can supply essential clues which may lead to important clinical applications (see Chapter 9).
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Holland, Peter W. H. "Cloning Genes Using the Polymerase Chain Reaction." In Essential Developmental Biology. Oxford University PressOxford, 1993. http://dx.doi.org/10.1093/oso/9780199634231.003.0025.

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Abstract The polymerase chain reaction (PCR) is an in vitro method for amplifying regions of DNA, utilizing information based on nucleotide sequences flanking the region of interest (1-3). PCR is used in the study of DNA sequence variation, genomic organization or gene expression (the latter using cDNA as a template, reverse transcribed from RNA; Chapter 24). PCR can also be used to amplify DNA from previously uncharacterized genes, if predictions can be made regarding the sequence of part of the gene(s).
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Konferenzberichte zum Thema "CAV-3 gene"

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K. Alkhudhairy, Miaad, та Elhassan Benyagoub. "Frequency of Genes Mediated β-lactams Resistance in Acinetobacter Baumannii Isolates from Iraq". У X INTERNATIONAL CONGRESS OF PURE AND APPLIED TECHNOLOGICAL SCIENCES. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress10-2.

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Background: Acinetobacter baumannii is a nosocomial virulent microorganism that can cause acute and chronic infections in burn patients. The aim of the study: Diagnosis of genes mediated β-lactams resistance among test isolates. Materials and Methods: 649 swabs collected from inpatients with burn-wound infections at a burn center in Al-Najaf Province/ Iraq, from August 2022 to February 2023. Results: 68/ 649 (10.5%) isolates of Acinetobacter baumannii were identified according to microscopically, cultural, and biochemical features. 22 (32.4%) isolates were found able to produce extended-spectr
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Olton, Dana, Dong Hyun Lee, Charles Sfeir, and Prashant N. Kumta. "Novel Nanostructured Calcium Phosphate Based Delivery Systems for Non-Viral Gene Delivery." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176286.

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Calcium phosphate (CaP) based approaches remain an attractive option for delivering plasmid DNA (pDNA) into cultured cells [1, 2]. However, two major limitations associated with this vector exist. First, it yields lower transfection efficiencies with respect to its’ viral counterparts. Second, CaP mediated gene delivery leads to transient transgene expression. Thus, we hypothesized that these concerns could respectively be addressed by: (1) synthesizing particles with precise control of the materials’ parameters including (i.e. Ca/P ratio, particle size, crystal structure, and microstructure)
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Chakraborty, Amlan, Venkatakrishna R. Jala, Sutirtha Chakraborty, R. Eric Berson, M. Keith Sharp, and Bodduluri Haribabu. "Bidirectional Oscillatory Shear Stress Increases Pro-Atherogenic Gene Expressions (I-CAM1, E-Selectin and IL-6) in Endothelial Cells." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53747.

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Wall shear stress (WSS) plays a key role in altering intracellular pathways and gene expression of endothelial cells, and has significant impacts on atherosclerotic plaque development (1–3). Further, the atherogenic regulators Leukotriene B4 (LTB4) and Lipopolysaccharide (LPS) have significant impacts on the pathophysiology of many inflammatory diseases. This study investigates the effects of oscillatory shear directionality on pro-atherogenic gene expression (I-CAM, E-Selectin, and IL-6) in the presence of LTB4 and LPS. An orbital shaker was used to expose the endothelial cells to oscillatory
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Vaughan, Andrew T., Rebecca Wright, and Katrina Slemmons. "Abstract B35: Estradiol drives MLL gene fusions in infant acute leukemia." In Abstracts: AACR Special Conference: Pediatric Cancer at the Crossroads: Translating Discovery into Improved Outcomes; November 3-6, 2013; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.pedcan-b35.

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Bernaedi, F., V. Bertagnolo, S. Bartolai, L. Rossi, F. Panicucci, and F. Conconi. "A POINT MUTATION AND A GENE DELETION OF FVIII GENE IN SEVERE HAEMOPHILIA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644047.

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The presence of Factor VIII (FVIII) gene lesions has been investigated in 100 haemophilia A patients using cDNA probes for the 3'part of FVIII gene (exons 14-26 ).In two related severe patients without inhibitor a deletion removesthe exon 26; the gene lesion has been confirmed with several restriction enzymes and has been shown by densitometry of the autoradiographic pattern in a woman of the same family. The complete deletionof the exon 26 has been described by Gitschier et al. in a patient with inhibitor. Thus the comparison of the end points of the two deletions could help to define the mec
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Birch, David G., Gabriel H. Travis, Kirsten G. Locke, and Donald C. Hood. "Rod Ergs in Mice and Humans with Putative Null Mutations in the RDS Gene." In Vision Science and its Applications. Optica Publishing Group, 1997. http://dx.doi.org/10.1364/vsia.1997.ma.4.

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Mutations causing autosomal dominant retinitis pigmentosa (RP) have been identified in RDS, the human homologue of the gene that was first identified and isolated as the cause of mouse "retinal degeneration slow" or rds (1, 2). The rds gene encodes rds/peripherin, an integral membrane glycoprotein located in outer segment disks (1, 3, 4). More than 15 distinct disease-causing mutations in the RDS gene have been reported (5). The clinical phenotypes include adRP, dominant retinitis punctata albescens, dominant butterfly shaped pigment dystrophy of the fovea and autosomal dominant macular degene
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Bhardwaj, Anjana, Nivetha Ganesan, Constance T. Albarracin, and Isabelle Bedrosian. "Abstract A115: Annexin A1: A novel gene target of miRNA-21." In Abstracts: AACR Special Conference on Advances in Breast Cancer Research: Genetics, Biology, and Clinical Applications - October 3-6, 2013; San Diego, CA. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1557-3125.advbc-a115.

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Badia, Bruno de Mattos Lombardi, Roberta Ismael Lacerda Machado, Wladimir Bocca Vieira de Rezende Pinto, et al. "Blurred Lines – Is the distinction between CIDP and CMT always clear?" In XIII Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2021. http://dx.doi.org/10.5327/1516-3180.015.

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Introduction: Charcot-Marie-Tooth disease (CMT) is a group of inherited sensorymotor neuropathies with variable age of onset, clinical and neurophysiological patterns but often with a chronic slow progression. Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is an immune-mediated neuropathy with a relapsing clinical course and typically good response to corticosteroids or other therapies. The distinction between these two conditions can be made with aid of clinical history, neurophysiological studies and genetic testing in the vast majority of cases. However, an overlap between
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O'hara, Patrick J., Frank A. Grant, A. Betty, J. Haldmen, and Mark J. Murray. "Structure of the Human Factor VII Gene." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643786.

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Factor VII is a member of a family of vitamin K-dependent, gamma-carboxylated plasma protein which includes factor IX, factor X, protein C, protein S and prothrombin. Activated factor VII (factor Vila) is a plasma serine protease which participates in a cascade of reactions leading to the coagulation of blood. Two overlapping genomic clones containing sequences encoding human factor VII were isolated and characterized. The complete sequence of the gene was determined and found to span 12.8 kilobases. The mRNA for factor VII as demonstrated by cDNA cloning is polyadenylated at multiple sites bu
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Fleming, Paul, and Tara Dalton. "One-Step Reverse-Transcription PCR on a High-Throughput Micro-Fluidic Device." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206623.

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One step reverse-transcription polymerase chain reaction (RT-PCR) assays are an attractive option for further automating gene detection assays. One-step assays can reduce hands–on-time and the risk of sample crossover and contamination. The one-step chemistries are showing increasing use in virus detection and have been reported, in some cases, to be more appropriate than their two-step counterparts [1, 2]. Previous work presented by the Stokes Institute research group outlined a micro fluidic based continuous flow instrument which performed high throughput qPCR in nanolitre sized droplets [3]
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Berichte der Organisationen zum Thema "CAV-3 gene"

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Levin, Ilan, John W. Scott, Moshe Lapidot, and Moshe Reuveni. Fine mapping, functional analysis and pyramiding of genes controlling begomovirus resistance in tomato. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7594406.bard.

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Abstract. Tomato yellow leaf curl virus (TYLCV), a monopartitebegomovirus, is one of the most devastating viruses of cultivated tomatoes and poses increasing threat to tomato production worldwide. Because all accessions of the cultivated tomato are susceptible to these viruses, wild tomato species have become a valuable resource of resistance genes. QTL controlling resistance to TYLCV and other begomoviruses (Ty loci) were introgressed from several wild tomato species and mapped to the tomato genome. Additionally, a non-isogenic F₁diallel study demonstrated that several of these resistance sou
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Reisch, Bruce, Avichai Perl, Julie Kikkert, Ruth Ben-Arie, and Rachel Gollop. Use of Anti-Fungal Gene Synergisms for Improved Foliar and Fruit Disease Tolerance in Transgenic Grapes. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7575292.bard.

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Original objectives . 1. Test anti-fungal gene products for activity against Uncinula necator, Aspergillus niger, Rhizopus stolonifer and Botrytis cinerea. 2. For Agrobacterium transformation, design appropriate vectors with gene combinations. 3. Use biolistic bombardment and Agrobacterium for transformation of important cultivars. 4. Characterize gene expression in transformants, as well as level of powdery mildew and Botrytis resistance in foliage of transformed plants. Background The production of new grape cultivars by conventional breeding is a complex and time-consuming process. Transfer
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Palukaitis, Peter, Amit Gal-On, Milton Zaitlin, and Victor Gaba. Virus Synergy in Transgenic Plants. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573074.bard.

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Transgenic plants expressing viral genes offer novel means of engendering resistance to those viruses. However, some viruses interact synergistically with other viruses and it is now known that transgenic plants expressing particular genes of one virus may also mediate synergy with a second virus. Thus, our specific objectives were to (1) determine if transgenic plants resistant to one virus showed synergy with another virus; (2) determine what viral sequences were essential for synergy; and (3) determine whether one of more mechanisms were involved i synergy. This project would also enable an
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Grumet, Rebecca, Rafael Perl-Treves, and Jack Staub. Ethylene Mediated Regulation of Cucumis Reproduction - from Sex Expression to Fruit Set. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7696533.bard.

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Reproductive development is a critical determinant of agricultural yield. For species with unisexual flowers, floral secualdifferentation adds additional complexity, that can influenec productivity. The hormone ethylene has long, been known to play a primary role in sex determination in the Cucumis species cucumber (C. sativus) and melon (C. melo). Our objectives were to: (1) Determine critical sites of ethylene production and perception for sex determination; (2) Identify additional ethylene related genes associated with sex expression; and (3) Examine the role of environment ami prior fruit
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Xu, Jin-Rong, and Amir Sharon. Comparative studies of fungal pathogeneses in two hemibiotrophs: Magnaporthe grisea and Colletotrichum gloeosporioides. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7695585.bard.

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Plant pathogenic fungi have various life styles and different plant infection strategies. Hemibiotrophs like Magnaporthe grisea and Colletotrichum species develop specialized structures during plant infection. The goal of this study was to identify, characterize, and compare genes required for plant infection in M. grisea and C. gloeosporioides. Specific objectives are to: 1) further characterize genes identified in the preliminary studies of C. gloeosporioides and M. grisea;2) identify and characterize additional fungal genes tagged by GFP; and 3) identify in planta growth and appressorium-sp
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Gal-On, Amit, Shou-Wei Ding, Victor P. Gaba, and Harry S. Paris. role of RNA-dependent RNA polymerase 1 in plant virus defense. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597919.bard.

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Objectives: Our BARD proposal on the impact of RNA-dependent RNA polymerase 1 (RDR1) in plant defense against viruses was divided into four original objectives. 1. To examine whether a high level of dsRNA expression can stimulate RDR1 transcription independent of salicylic acid (SA) concentration. 2. To determine whether the high or low level of RDR1 transcript accumulation observed in virus resistant and susceptible cultivars is associated with viral resistance and susceptibility. 3. To define the biogenesis and function of RDR1-dependent endogenous siRNAs. 4. To understand why Cucumber mosai
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Friedman, Haya, Julia Vrebalov, James Giovannoni, and Edna Pesis. Unravelling the Mode of Action of Ripening-Specific MADS-box Genes for Development of Tools to Improve Banana Fruit Shelf-life and Quality. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592116.bard.

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Fruit deterioration is a consequence of a genetically-determined fruit ripening and senescence programs, in which developmental factors lead to a climacteric rise of ethylene production in ethylene-sensitive fruits such as tomato and banana. Breeding of tomato with extended fruit shelf life involves the incorporation of a mutation in RIN, a MADS-box transcription factor participating in developmental control signalling of ripening. The RIN mode of action is not fully understood, and it may be predicted to interact with other MADS-box genes to execute its effects. The overall goal of this study
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Meir, Shimon, Michael Reid, Cai-Zhong Jiang, Amnon Lers, and Sonia Philosoph-Hadas. Molecular Studies of Postharvest Leaf and Flower Abscission. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696523.bard.

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Original objectives: Understanding the regulation of abscission competence by exploring the nature and function of auxin-related gene expression changes in the leaf and pedicelAZs of tomato (as a model system), was the main goal of the previously submitted proposal. We proposed to achieve this goal by using microarray GeneChip analysis, to identify potential target genes for functional analysis by virus-induced gene silencing (VIGS). To increase the potential of accomplishing the objectives of the previously submitted proposal, we were asked by BARD to show feasibility for the use of these two
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Stern, David B., and Gadi Schuster. Manipulation of Gene Expression in the Chloroplast: Control of mRNA Stability and Transcription Termination. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7568750.bard.

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Chloroplasts are the site of photosynthesis and of other essential biosynthetic activities in plant cells. Chloroplasts are semi-autonomous organelles, since they contain their own genomes and protein biosynthetic machinery, but depend on the coordinate expression of nuclear genes to assemble macromolecular complexes. The bioeingineering of plants requires manipulation of chloroplast gene expression, and thus a knowledge of the molecular mechanisms that modulate mRNA and protein production. In this proposal the heterotrophic green alga Chlamydomonas reinhardtii has been used as a model system
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Wang, X. F., and M. Schuldiner. Systems biology approaches to dissect virus-host interactions to develop crops with broad-spectrum virus resistance. United States-Israel Binational Agricultural Research and Development Fund, 2020. http://dx.doi.org/10.32747/2020.8134163.bard.

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More than 60% of plant viruses are positive-strand RNA viruses that cause billion-dollar losses annually and pose a major threat to stable agricultural production, including cucumber mosaic virus (CMV) that infects numerous vegetables and ornamental trees. A highly conserved feature among these viruses is that they form viral replication complexes (VRCs) to multiply their genomes by hijacking host proteins and remodeling host intracellular membranes. As a conserved and indispensable process, VRC assembly also represents an excellent target for the development of antiviral strategies that can b
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