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Zeitschriftenartikel zum Thema "Candida albicans – diet therapy"
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Fesharaki, Shirinsadat Hashemi, Iman Haghani, Bita Mousavi, Melika Laal Kargar, Mohammadali Boroumand, Maryam Sotoudeh Anvari, Kyomars Abbasi, Jacques F. Meis und Hamid Badali. „Endocarditis due to a co-infection of Candida albicans and Candida tropicalis in a drug abuser“. Journal of Medical Microbiology 62, Nr. 11 (01.11.2013): 1763–67. http://dx.doi.org/10.1099/jmm.0.060954-0.
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In recent decades the incidence of Candida endocarditis has increased dramatically. Despite the application of surgery and antifungal therapy, Candida endocarditis remains a life-threatening infection with significant morbidity and mortality. We report a 37-year-old male drug abuser presenting with high fever, chest pain, loss of appetite and cardiac failure. His echocardiography revealed mobile large tricuspid valve vegetations. Fungal endocarditis was confirmed by culturing of the resected vegetation showing mixed growth of Candida albicans and Candida tropicalis, although three consecutive blood cultures were negative for Candida species. Phenotypic identification was reconfirmed by sequencing of the internal transcribed spacer (ITS rDNA) region. The patient was initially treated with intravenous fluconazole (6 mg kg−1 per day), followed by 2 weeks of intravenous amphotericin B deoxycholate (1 mg kg−1 per day). Although MICs were low for both drugs, the patient’s antifungal therapy combined with valve replacement failed, and he died due to respiratory failure.
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Öncü, Bahaettin, Nurşen Belet, Ahmet Naci Emecen und Asuman Birinci. „Health care-associated invasive Candida infections in children“. Medical Mycology 57, Nr. 8 (20.03.2019): 929–36. http://dx.doi.org/10.1093/mmy/myz005.
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Abstract The aims of the study were to examine the distribution of Candida spp. isolated from sterile body sites, the antifungal susceptibility of the isolates to amphotericin B, and fluconazole, risk factors and clinical outcomes associated with invasive health care-associated Candida infections in neonates and children. Between January 2007 and January 2012, the patients with invasive candidiasis were detected from microbiology laboratary records and medical records were examined retrospectively. Candida spp. were isolated from sterile body sites in 94 patients. The most common underlying diseases were prematurity in neonates and surgery in children. Parenteral nutrition, stay in intensive care unit (ICU), and mechanical ventilation (MV) were major risk factors in neonates. Hospitalization before infection and immunosuppressant therapy were significantly more frequent in children. Of Candida infection episodes, 29.8% was due to C. albicans and 70.2% was due to non-albicans Candida spp. The most common isolated species was C. parapsilosis. Of the Candida species, 90.8% were sensitive, and 9.2% were resistant to fluconazole. The rate of amphotericin B resistant was 1.3%; 23.4% of the patients died in the first 30 days. The main variables associated with mortality were neonates, prematurity, stay in the ICU, parenteral nutrition, MV, length of stay, amphotericin B susceptibility, and high levels of C-reactive protein.
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Oreiro, Mariana Bastos, Miguel Canales, Julio García Rodríguez, Raquel de Paz, Ana Lopez de la Guia, Monica Martin Salces, Peter Lang, Jose Ramon Paño Pardo und Fernando Hernandez Navarro. „Candidemia in Patients with Hematological Malignancies: The Role of Prophilaxis and the Importance of Local Epidemiology for Treatment“. Blood 112, Nr. 11 (16.11.2008): 4839. http://dx.doi.org/10.1182/blood.v112.11.4839.4839.
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Abstract Introduction: Candidemia is a serious condition with a high mortality rate in patients with hematological malignancies. It is thus important to understand the associated risk factors, as well as the need to establish adequate prophylaxis and early, effective therapy. The objective of this study was to determine the incidence of candidemia in hospital patients with hematological malignancies; to describe its clinical features and the risk factors associated with infection and with a poor outcome. Materials and methods: An electronic database was used to identify cases with a positive blood culture for Candida spp in patients with hematological malignancies admitted to the Hematology Ward of Hospital Universitario La Paz between January 2000 to March 2008. The clinical history of each identified case was reviewed. SPSS 15.0 was used for the statistical analysis. Univariant analysis was carried out using χ2. Results: Forty seven patients were identified, with an annual incidence of 1%. The species identified were Candida parapsilopsis in 46% of cases (n = 22) and Candida albicans in 21.3% (n = 10); the remainder was distributed amongst C. guillermondi, C. tropicalis and C. krusei. The underlying hemalogic malignancies were non-Hodgkin lymphoma (34%, n = 16), multiple myeloma (19%, n = 9) and acute myeloid leukemia (17%, n = 8). 48.9% of patients underwent stem cell transplantation (45.3% allogeneic and 54.7% autologous). No significant association was found between the underlying hemalogic malignancy and the species of Candida that was isolated. The antifungals used in treatment were liposomal amphotericin in 48.9% of cases, fluconazole in 12.7%, caspofungin in 4.2% and voriconazole in 4.2%, with combined therapy in 30% of patients. MIC50 and MIC90 for fluconazole against C. parapsilopsis were 4 and 32, respectively, and 0.03 and 8, respectively against C. albicans. MIC90 against the other species was 0.03. MIC50 and MIC90 for amphotericin were 0.03 and 1, respectively, against C. albicans, C. parapsilopsis and C. krusei. Voriconazole, itraconazole and caspofungin were found to have an MIC90 of 0.03 against all species of Candida. Thirty seven point eight percet of patients were already receiving antifungal prophylaxis at the time of diagnosis of candidemia, although 90% of cases of C. albicans candidemia were not on prophylaxis (p<0.05). In terms of risk factors, 76.6% of patients had a central venous catheter, 78.8% were undergoing chemotherapy, 95.6% were receiving concomitant, broad-spectrum antibiotics, 21.7% were diabetic, 46.8% were receiving parenteral nutrition of which more than half (59.1%) were associated with C. parapsilopsis, 26.7% had a serious associated mucositis, 60.5% had less than 0.2 ×109/L neutrophils and 98% had less than 1.5 × 109/L neutrophils, 37% had kidney failure. Eight patients (17%) died as a result of candidemia: 4 from C. albicans, 2 from C. parapsilopsis, 1 from C. glabrata and 1 from C. krusei. Of the patients with C. albicans, 33% died, compared to 11.8% of those with other species of Candida (p<0.05). Conclusion: Candida parapsilopsis was found to be the main causative species of candidemia in our centre, with a markedly high MIC50 and MIC90 for fluconazole, probably related to fluconazole prophylaxis. These findings highlight the importance of understanding the epidemiology of each centre when planning treatment and establishing an effective scheme of prophylaxis in high-risk patients to avoid the mortality associated with this type of infectious complication
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Park, S. S., D. J. D'Amico, B. Paton und A. S. Baker. „Treatment of exogenous Candida endophthalmitis in rabbits with oral fluconazole.“ Antimicrobial Agents and Chemotherapy 39, Nr. 4 (April 1995): 958–63. http://dx.doi.org/10.1128/aac.39.4.958.
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We investigated the efficacy of oral fluconazole, alone or in combination with oral flucytosine (5FC), in treating Candida endophthalmitis using a rabbit model. Albino rabbits were infected with an intravitreal inoculation of 1,000 CFU of susceptible Candida albicans and randomized 5 days later to receive treatment with oral fluconazole alone (80 mg/kg of body weight per day), a combination of fluconazole and 5FC (100 mg/kg/12 h), or no treatment. The treatment effect was assessed at 2 and 4 weeks after therapy by funduscopy, quantitative vitreous culture, and histopathology. Intravitreal levels of fluconazole, 2 to 24 h after the first dose, were measured to be > 10 times the MIC of the drug for C. albicans. Among rabbits treated with fluconazole for 2 weeks, 67% had a > 90% reduction in fungal load (P < 0.05) and 33% were sterile. After 4 weeks, all had a > 99% reduction in fungal load (P < 0.05) and 75% were sterile (P = 0.01). This treatment effect was unchanged 4 weeks after discontinuation of fluconazole. Among rabbits treated with fluconazole and 5FC for 2 weeks, 67% died during therapy. Among the surviving rabbits, 75% had a > 90% reduction in fungal load (P < 0.05) and 25% were sterile. We conclude that oral fluconazole may be useful for treatment of Candida endophthalmitis. Addition of 5FC was associated with high toxicity and minimal additional antifungal effect in our rabbit model.
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Assaf, Areej M., Bassam I. Amro, Sundus Mashallah und Randa N. Haddadin. „Antimicrobial and anti-inflammatory potential therapy for opportunistic microorganisms“. Journal of Infection in Developing Countries 10, Nr. 05 (31.05.2016): 494–505. http://dx.doi.org/10.3855/jidc.7610.
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Introduction: Methanolic extracts of six plants (Arbutus andrachne, Chrysanthemum coronarium, Inula viscosa, Origanum syriacum, Punica granatum, and Rosmarinus officinalis) used in traditional medicine for the treatment of bacterial and fungal infections were evaluated. The present study was conducted to evaluate the antimicrobial and anti-inflammatory activity of some medicinal plants in lowering the risk of opportunistic infections of the oral cavity caused by Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Extracts were evaluated separately and in a mixture. Methodology: The methanolic plant extracts were tested against three opportunistic microorganisms by determining the minimum inhibitory concentration (MIC). They were also evaluated for their ability to suppress the release of the pro-inflammatory cytokine IL-6 while not suppressing the release of the anti-inflammatory cytokine IL-10 from peripheral blood mononuclear cells using ELISA. Results: All extracts showed both antimicrobial and anti-inflammatory activities. However, O. syriacum exhibited the highest antimicrobial activity for the three microorganisms among all of the tested extracts (MIC S. aureus: 1 mg/mL; P. aeruginosa: 2 mg/mL; and C. albicans: 1 mg/mL). The extracts inhibited the expression of the pro-inflammatory cytokine IL-6 with apparent dose-dependent responses while they attenuated the secretion of the anti-inflammatory cytokine IL-10. The mixture of O. syriacum and R. officinalis showed an anti-inflammatory effect, with a synergistic antimicrobial effect. Conclusion: These findings support the idea that a diet rich in plants and herbs may contribute to the reduction of inflammation and microbial growth and may also be preventive against various infections, including those related to the oral cavity.
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Ahmed, Abeera, Nargis Daud, Lahore Gohar Zaman, Aamer Ikram und Muhammed Tahir Khadim. „Candidemia: Predisposing Factors, Antifungal Susceptibility, Clinical Outcome and Connotations for Management“. Infection Control & Hospital Epidemiology 41, S1 (Oktober 2020): s148. http://dx.doi.org/10.1017/ice.2020.666.
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Objective: We conducted this study to investigate the epidemiology of candidemia in our setting and to quantify the risk factors associated with disease, overall outcome, and mortality associated with candidemia. Methods: In this prospective observational study, we conducted lab-based surveillance with clinical correlation of all cases of candidemia within our ICUs during the period (2016–2018). Clinical assessment was done on day 5 and day 30, and comorbidities, clinical features, and outcome were observed within 30 days after the diagnosis. The diagnosis was made on the basis of positive blood culture for Candida spp and a compatible clinical picture. The demographic characteristics, sequential organ failure assessment (SOFA) scores, comorbidities, use of invasive devices, antibiotics administered were observed, and antifungal susceptibility testing was performed according to CLSI guidelines. Type and duration of antifungal administered and outcomes were noted. Results: In total, 48 episodes of candidemia, with 29 (60%) males and 19 (40%) females, were identified during the study period. C. albicans was the most common specie responsible for candidemia, causing 17 of the cases (~35%), whereas rest of the cases were caused by non–albicans spp, which included C. auris, accounting for 9 (19%) C. parapsilosis and C. tropicalis 7 (15%) each, C. glabrata and C. famata 2 (6%), and C. krusei was isolated in only 2 cases (4%). Among modifiable risk factors, CVC insertion and antibiotic exposure were the leading factors, seen in 100% of patient. Candida colonization was observed in 26 patients (28%), of whom 2 (4%) had multifocal Candida colonization. Among evaluable patients, 17 (35%) died within 30 days of the onset of candidemia. C. tropicalis was associated with the highest mortality rate, 27% (n = 4) in this cohort. Regarding the crude mortality in the different units, patients in medical ICU had the highest mortality rate (54%). In vitro activity of 3 systemically active antifungal agents was tested against 48 isolates of Candida spp. Based on CLSI break points, the susceptibility to voriconazole was 98%; only 1 isolate was resistant to voriconazole. Among candidemia-positive cases, 28 patients (58%) had taken the antifungals for >14 days, whereas 18 (37.5%) were treated for <14 days and 2 (4%) died before the initiation of therapy. Conclusions: In our study, C. albicans was the most common specie responsible for candidemia, but non–albicans spp are also emerging, with higher in vitro resistance to antifungals.Funding: NoneDisclosures: None
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Koncar, Igor, Marko Dragas, Predrag Sabljak, Predrag Pesko, Miroslav Markovic und Lazar Davidovic. „Aortoesophageal and aortobronchial fistula caused by Candida albicans after thoracic endovascular aortic repair“. Vojnosanitetski pregled 73, Nr. 9 (2016): 864–67. http://dx.doi.org/10.2298/vsp141209074k.
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Introduction. Endovascular stent-graft placement has emerged as a minimally invasive alternative to open surgery for the treatment of aortic aneurysms and dissections. There are few reports of stent graft infections and aortoenteric fistula after endovascular thoracic aortic aneurysm repair, and the first multicentric study (Italian survey) showed the incidence of about 2%. Case report. We presented a 69-year-old male patient admitted to our hospital 9 months after thoracic endovascular aortic repair, due to severe chest pain in the left hemithorax and arm refractory to analgesic therapy. Multislice computed tomography (MSCT) showed a collection between the stent graft and the esophagus with thin layers of gas while gastroendoscopy showed visible blood jet 28 cm from incisive teeth. Surgical treatment was performed in collaboration of two teams (esophageal and vascular surgical team). After explantation of the stent graft and in situ reconstruction by using Dacron graft subsequent esophagectomy and graft omentoplasty were made. After almost four weeks patient developed hemoptisia as a sign of aorto bronchial fistula. Treatment with implantation of another aortic cuff of 26 mm was performed. The patient was discharged to the regional center with negative blood culture, normal inflammatory parameters and respiratory function. Three months later the patient suffered deterioration with the severe weight loss and pneumonia caused by Candida albicans and unfortunately died. The survival time from the surgical treatment of aortoesophageal fistula was 4 months. Conclusion. Even if endovascular repair of thoracic aortic diseases improves early results, risk of infection should not be forgotten. Postoperative respiratory deterioration and finally hemoptisia could be the symptoms of another fistula.
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Khan, Fahmi Yousef, Mohammed Elsayed, Deshmukh Anand, Mohammed Abu Khattab und Doiphode Sanjay. „Fungal peritonitis in patients undergoing continuous ambulatory peritoneal dialysis in Qatar“. Journal of Infection in Developing Countries 5, Nr. 09 (05.07.2011): 646–51. http://dx.doi.org/10.3855/jidc.1519.
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Introduction: This study was conducted at Hamad General Hospital to determine the incidence of fungal peritonitis and to describe its clinical and microbiological findings in patients undergoing continuous ambulatory peritoneal dialysis in Qatar. Methodology: The medical records of these patients between 1 January 2005 and 31 December 2008 were retrospectively reviewed and the collected data were analysed. Results: During the study period, 141 episodes of peritonitis were observed among 294 patients. In 14 of these episodes (9.9%), fungal peritonitis was reported in 14 patients with a rate of 0.05 episodes per patient year, while the bacterial peritonitis rate was 0.63 per patient year. Thirteen (93%) patients had one or more previous episodes of bacterial peritonitis that was treated with multiple broad-spectrum antibiotics, 11 (85%) had received broad-spectrum antibiotics within the preceding month, 12 (92%) within three months, and 8 (62%) within six months. Candida species were the only fungal species isolated from the dialysate with predominance of non-albicans Candida species (especially Candida parapsilosis). Therapeutic approach was immediate catheter removal, followed by systemic antifungal therapy and temporary haemodialysis. Nine patients (64.3%) were continued on haemodialysis, whereas five patients (35.7%) died. Conclusions: Prior antibiotic use was an important risk factor predisposing patients to the development of fungal peritonitis. Early detection of fungal peritonitis would lead to early institution of appropriate therapy and prevention of complications.
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Caulier, Thomas, Olivier Robineau, Armelle Pasquet, Pier-Vito D’Elia, Barthelemy Lafon-Desmurs, Olivier Leroy, Serge Alfandari und Eric Senneville. „1029. Outcome of Candida Graft Vascular Infection: Results From a Prospective Cohort“. Open Forum Infectious Diseases 5, suppl_1 (November 2018): S307. http://dx.doi.org/10.1093/ofid/ofy210.866.
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Abstract Background Candida graft vascular infections (CGVI) are rare events and little data are available in the literature. The aim of this study was to describe the characteristics and outcome of patients admitted for fungal graft vascular infections, in a reference center for CGVI treatment. Methods Patients admitted for a CGVI in our center from 1 January 2000 to 1 February 2018 were prospectively included. Clinical, biological, and outcome data were recorded. Results Two hundred patients were admitted with graft vascular infections (GVI) in our center, and 11 of them (6%) presented CGVI. They were mainly men (7; 64%), and median age was 74 years old [min–max: 39–83]. All patients had benefited from prosthetic bypass surgery prior to CGVI, and infection was considered as an early disease in six patients (55%). Candida albicans was found in 72% of cases. Infection was plurimicrobial in 10 patients (92%), involving Staphyloccocus aureus in only one case and Bacille gram negatif in six (55%) cases. The management consisted in a total or partial graft replacement for five patients (45%), and surgical revision was required in four of them (30%). The empirical antifungal therapy included an echinocandin (Caspofungine) for eight patients (73%), and was changed to fluconazole or voriconazole according to antifungigram. Two patients received Amphotericin B therapy, complicated by acute kidney injury. Intensive care unit admittance was required for nine patients (82%). After the curative treatment period, antifungal therapy could not be removed in two patients and was long-continued using fluconazole. Finally, six patients (55%) died, all within the year after CGVI. Conclusion To our knowledge, we report here the biggest CGVI cohort. CGVI resulted in very high morbidity and mortality, requiring ICU admission for a long time. Despite multidisciplinary management involving anesthesiologists, surgeons, intensive care, and infectious disease physicians, outcome of CGVI patients remains poor. Disclosures All authors: No reported disclosures.
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Vassilis, Vargemezis Zoe L., Homer Papadopoulou, Anna-Maria Liamos, Taisir Natscheh Belechri, George Vergoulas, Rodothea Antoniadou, Vassilis Kilintzis und Menelaos Papadimitriou. „Management of Fungal Peritonitis during Continuous Ambulatory Peritoneal Dialysis (CAPD)“. Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 6, Nr. 1 (Januar 1986): 17–20. http://dx.doi.org/10.1177/089686088600600106.
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A group of 138 patients (pts) on CAPD for a total of 1889 patientmonths had 176 episodes of peritonitis. Eighteen (13%) had fungal peritonitis at one to 20 months after beginning CAPD. The responsible organisms were Candida albicans (nine pts), Candida parapsilosis (five), Candida torulopsis (three), and Trichosporum capitatum (one). These infections were treated by various combinations of antifungal agents using two different strategies: Removal of the Tenckhoff catheter and interruption of CAPD for 10 to 14 days in nine pts (Group A), and continuous peritoneal lavage for four to six days in the remainder -nine pts (Group B). Fourteen of the 18 were cured (seven of nine in each group). In group A, two patients died, one continues on CAPD while the other six (67%) were transferred to hemodialysis because they developed peritoneal adhesions with significant loss in peritoneal space. In group B, only two patients have been transferred to hemodialysis; the other seven continue on CAPD. In our experience, the preferred initial form of treatment is continuous peritoneal lavage in conjunction with antifungal therapy. Catheter removal should be reserved for those who fail to respond to this treatment. Continuous ambulatory peritoneal dialysis (CAPD) is now accepted as a alternative treatment for end-stage renal disease. However, despite continuous progress in this field, peritonitis still remains a major complication and results in increased morbidity and cost. Although pathogenic fungi account for only 2 7% of peritonitis in patients on CAPD (1–3), the illness is severe and represents a major clinical challenge to these patients. In addition, the therapy of fungal peritonitis is still controversial, especially with regard to the need for removal of the catheter. This paper presents a retrospective study and describes our experience during the past four years with the management of fungal peritonitis in 18 patients undergoing CAPD at our center.
Dissertationen zum Thema "Candida albicans – diet therapy"
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Martins, Joyce da Silva [UNESP]. „Efeitos da terapia fotodinâmica sobre a patogenicidade de Candida albicans e C dubliniensis na candidose bucal em ratos“. Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/95051.
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martins_js_me_sjc.pdf: 608313 bytes, checksum: e32f819f13bdf398082ecb9e208a1b5a (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O objetivo deste estudo foi avaliar os efeitos da terapia fotodinâmica sobre a patogenicidade de C. albicans e C. dubliniensis na candidose bucal em ratos. Foram utilizados 96 ratos (Rattus norvegicus, Albinus, Wistar), sendo que 80 animais foram submetidos à indução de candidose experimental no dorso da língua por C. albicans ou C. dubliniensis e 16 ratos não foram infectados por Candida. Após 5 dias da indução da candidose, foram realizados diferentes tratamentos: laser e azul de metileno como fotossensibilizador (Grupo L+F+), apenas laser (Grupo L+F-), apenas fotossensibilizador (Grupo L-F+) e grupo sem laser e fotossensibilizador (Grupo L-F-). Após 1 dia, amostras do dorso da língua foram coletadas e semeadas em ágar Sabouraud para contagem de UFC/mL recuperadas da cavidade bucal. Os animais foram submetidos à eutanásia para análise microscópica do dorso da língua. Foi avaliada também a capacidade de produção de fosfolipase e proteinase por C. albicans e C. dubliniensis. Os resultados demonstraram que a quantidade de C. albicans recuperadas da cavidade bucal dos ratos foram semelhantes entre os quatro grupos estudados. C. dubliniensis não foi recuperarada da cavidade bucal dos ratos estudados. Na análise microscópica do dorso da língua, observou-se a presença de várias lesões teciduais induzidas por C. albicans, sendo que no grupo L+F+ essas lesões foram menores em relação aos demais grupos estudados. C. dubliniensis exibiu lesões teciduais discretas sem diferença estatística entre os grupos. Em relação aos fatores de patogenicidade de C. albicans, o grupo L+F+ produziu menor quantidade de fosfolipase e proteinase em relação aos demais grupos. C. dubliniensis produziu menor quantidade de fosfolipase em relação à C. albicans, sem diferença entre os 4 grupos estudados. Entretanto, C. dubliniensis não foi capaz de produzir proteinase...
The objective was to evaluate the effects of photodynamic therapy on the pathogenicity of C. albicans and C. dubliniensis in oral rats candidosis. Were evaluated 96 rats, while 80 were subjected to candidosis experimental induction on the tongue dorsum by C. albicans or C. dubliniensis and 16 rats were not infected. After 5 days of induction candidosis, different treatments were performed: laser and methylene blue as photosensitizers (Group L+F+), laser only (Group L+F-), only photosensitizers (Group L-F+) and without laser neither photosensitizers group (Group L-F). After 1 day, samples of the tongue dorsum were collected and sown on Sabouraud agar for counts of UFC/mL recovered from the oral cavity. The animals were submitted to euthanasia for microscopic analysis. It also evaluated the production capacity of a proteinase and phospholipase C. albicans and C. dubliniensis. The results showed that the amount of C. albicans recovered from rats oral cavity were similar among the four groups. To recover C. dubliniensis of the oral cavity from studied rats was not possible. In microscopic examination of tongue dorsum, was seen the presence of multiple tissue lesions induced by C. albicans, whereas in group L+ F+ these lesions were smaller than in other groups. C. dubliniensis showed slight tissue lesions with no statistic difference between groups. Regarding the factors of pathogenicity of C. albicans, the group L+F+ produced smaller amounts of phospholipase and proteinase compared to other groups. C. dubliniensis produced fewer quantitiy of phospholipase related to C. albicans, without difference between the 4 (four) groups studied. However, C. dubliniensis was not able to produce proteinase. It was concluded that the development of experimental candidosis by C. dubliniensis was less than that induced by C. albicans. Photodynamic therapy was effective on treatment... (Complete abstract click electronic access below)
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Pereira, Cristiane Aparecida [UNESP]. „Efeitos da terapia fotodinâmica in vitro em biofilmes formados por Candida albicans, Staphylococcus aureus e Streptococcus mutans“. Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/87936.
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pereira_ca_me_sjc.pdf: 3427201 bytes, checksum: 7d684064d1dd59c89bf2d7b2b63a765e (MD5)O objetivo do trabalho foi avaliar o efeito antimicrobiano da terapia fotodinâmica (TFD) em biofilmes formados por C. albicans (GI), S. aureus (GII) e S. mutans (GIII), isolados e em associações (GIV-VII). Os biofilmes foram formados em discos de resina acrílica esterilizados imersos em caldo de infusão cérebro coração com 5% de sacarose, inoculados com a suspensão microbiana e incubados por 5 dias. Após o período de incubação, os discos foram lavados com solução fisiológica esterilizada para remover as células não-aderidas. Foram avaliados os efeitos do fotossensibilizador azul de metileno (AM) na concentração de 0,1 mg/mL por 5 min e laser AsGaAl (660 nm) por 98 s, isolados e em conjunto. Os biofilmes foram desprendidos em solução fisiológica em agitador ultrasônico. Foram realizadas diluições e alíquotas semeadas em ágar seletivos e incubadas por 48 h. Os números de UFC/mL em Log10 foram analisados estatisticamente (ANOVA, teste de Tukey, p< 0.05). Também foi realizada a microscopia eletrônica de varredura (MEV) nos discos com biofilmes dos grupos controle e TFD. Foram observadas reduções significativas na viabilidade de todos os biofilmes expostos ao AM e laser. As reduções (log10) foram maiores em biofilmes isolados, com: 40.22% para C. albicans (GI), 55.91% para S. aureus (GII) e 47.35% para S. mutans (GIII). Nos biofilmes mistos as reduções (log10) foram: 35.08% para C. albicans e 43.9% para S. aureus (GIV); 31.54% para C. albicans e 38.7% para S. mutans (GV); 40.12% para S. aureus e 37.14% para S. mutans (GVI); 19.56% para C. albicans, 28.41% para S. aureus e 24.94% para S. mutans (GVII). As imagens de MEV demonstraram uma fotossensibilização letal predominantemente nas camadas superiores dos biofilmes. Conclui-se que a TFD pode ser uma abordagem útil no controle de biofilmes bucais.
The purpose of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) in biofilms formed by C. albicans (GI), S. aureus (GII) and S. mutans (GIII), alone and in associations (GIVGVII). The biofilms were grown in acrylic resin discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension and incubated for 5 days. On the fifth day, the discs were washed in sterile saline solution in order to remove loosely bound material. The effects of the methylene blue (MB) photosensitizers at a concentration of 0,1 mg/mL for 5 min and AsGaAl laser (660 nm) for 98 s, alone and conjugated were evaluated. The dics were placed into tubes with sterile saline solution and sonicated for 30 s in order to disperse the biofilms. Ten-fold serial dilutions were carried out and aliquots plated in selective agar which were then incubated for 48 h. Then the numbers CFU/mL (log10) were counted and analyzed statistically (ANOVA, Tukey´s test, p< 0,05) Scanning electron microscopy (SEM) on discs with PDT and control biofilms groups were performed. Significant decreases in the viability of all microorganisms were observed when biofilms were exposed to both MB and laser. Reductions (log10) in single-species biofilms were greater than associated biofilms with: 40.22% for C. albicans (GI), 55.91% for S. aureus (GII) and 47.35% for S. mutans (GIII). In associated biofilms the reductions (Log10) were: 35.08% for C. albicans and 43.9% for S. aureus (GIV); 31.54% for C. albicans and 38.7% for S. mutans (GV); 40.12% for S. aureus and 37.14% for S. mutans (GVI); 19.56% for C. albicans, 28.41% for S. aureus and 24.94% for S. mutans (GVII). Scanning electron microscopy micrographs suggested that lethal photosensitization occurred predominantly in the outermost layers of the biofilms. It was concluded that PDT using MB photosensitizer and laser may be a useful approach in the control of oral biofilms.
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Martins, Joyce da Silva. „Efeitos da terapia fotodinâmica sobre a patogenicidade de Candida albicans e C dubliniensis na candidose bucal em ratos /“. São José dos Campos : [s.n.], 2009. http://hdl.handle.net/11449/95051.
Der volle Inhalt der QuelleAnnotation:
Orientador: Juliana Campos JunqueiraBanca: Antonio Olavo Cardoso Jorge
Banca: Martha Simões Ribeiro
Resumo: O objetivo deste estudo foi avaliar os efeitos da terapia fotodinâmica sobre a patogenicidade de C. albicans e C. dubliniensis na candidose bucal em ratos. Foram utilizados 96 ratos (Rattus norvegicus, Albinus, Wistar), sendo que 80 animais foram submetidos à indução de candidose experimental no dorso da língua por C. albicans ou C. dubliniensis e 16 ratos não foram infectados por Candida. Após 5 dias da indução da candidose, foram realizados diferentes tratamentos: laser e azul de metileno como fotossensibilizador (Grupo L+F+), apenas laser (Grupo L+F-), apenas fotossensibilizador (Grupo L-F+) e grupo sem laser e fotossensibilizador (Grupo L-F-). Após 1 dia, amostras do dorso da língua foram coletadas e semeadas em ágar Sabouraud para contagem de UFC/mL recuperadas da cavidade bucal. Os animais foram submetidos à eutanásia para análise microscópica do dorso da língua. Foi avaliada também a capacidade de produção de fosfolipase e proteinase por C. albicans e C. dubliniensis. Os resultados demonstraram que a quantidade de C. albicans recuperadas da cavidade bucal dos ratos foram semelhantes entre os quatro grupos estudados. C. dubliniensis não foi recuperarada da cavidade bucal dos ratos estudados. Na análise microscópica do dorso da língua, observou-se a presença de várias lesões teciduais induzidas por C. albicans, sendo que no grupo L+F+ essas lesões foram menores em relação aos demais grupos estudados. C. dubliniensis exibiu lesões teciduais discretas sem diferença estatística entre os grupos. Em relação aos fatores de patogenicidade de C. albicans, o grupo L+F+ produziu menor quantidade de fosfolipase e proteinase em relação aos demais grupos. C. dubliniensis produziu menor quantidade de fosfolipase em relação à C. albicans, sem diferença entre os 4 grupos estudados. Entretanto, C. dubliniensis não foi capaz de produzir proteinase... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The objective was to evaluate the effects of photodynamic therapy on the pathogenicity of C. albicans and C. dubliniensis in oral rats candidosis. Were evaluated 96 rats, while 80 were subjected to candidosis experimental induction on the tongue dorsum by C. albicans or C. dubliniensis and 16 rats were not infected. After 5 days of induction candidosis, different treatments were performed: laser and methylene blue as photosensitizers (Group L+F+), laser only (Group L+F-), only photosensitizers (Group L-F+) and without laser neither photosensitizers group (Group L-F). After 1 day, samples of the tongue dorsum were collected and sown on Sabouraud agar for counts of UFC/mL recovered from the oral cavity. The animals were submitted to euthanasia for microscopic analysis. It also evaluated the production capacity of a proteinase and phospholipase C. albicans and C. dubliniensis. The results showed that the amount of C. albicans recovered from rats oral cavity were similar among the four groups. To recover C. dubliniensis of the oral cavity from studied rats was not possible. In microscopic examination of tongue dorsum, was seen the presence of multiple tissue lesions induced by C. albicans, whereas in group L+ F+ these lesions were smaller than in other groups. C. dubliniensis showed slight tissue lesions with no statistic difference between groups. Regarding the factors of pathogenicity of C. albicans, the group L+F+ produced smaller amounts of phospholipase and proteinase compared to other groups. C. dubliniensis produced fewer quantitiy of phospholipase related to C. albicans, without difference between the 4 (four) groups studied. However, C. dubliniensis was not able to produce proteinase. It was concluded that the development of experimental candidosis by C. dubliniensis was less than that induced by C. albicans. Photodynamic therapy was effective on treatment... (Complete abstract click electronic access below)
Mestre
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Costa, Anna Carolina Borges Pereira da [UNESP]. „Avaliação da terapia fotodinâmica em candida albicans in vitro e in vivo“. Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/95047.
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costa_acbp_me_sjc.pdf: 3713548 bytes, checksum: 5d63f9c051b3f55b3f5296a1362fb144 (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Os objetivos do presente estudo foram avaliar a ação antifúngica da Terapia Fotodinâmica (TFD) em culturas planctônicas e biofilmes de Candida albicans formados in vitro e em modelo de candidose experimental em camundongos, bem como sua interferência na aderência de C. albicans às células epiteliais bucais humanas in vitro. Foi utilizada cepa padrão de C. albicans (ATCC 18804) para os ensaios. Como fonte de luz foi utilizado o Diodo Emissor de Luz (LED) com emissão de luz verde (532±10 nm) com potência de 90 mW e fotossensibilizador eritrosina nas concentrações variando de 200 a 0,39 μM para os ensaios em cultura plactônica. O biofilme foi formado em placas de 96 poços e tratado com o fotossensibilizador eritrosina na concentração de 400 μM e luz LED. 56 camundongos machos e adultos foram imunossuprimidos e inoculados com suspensões contendo 108 células/mL de C. albicans. Os animais foram submetidos a TFD mediada pelo corante eritrosina (400 μM) e irradiados por LED. Antes e após os tratamentos, foram recuperadas leveduras da cavidade bucal dos animais. As leveduras recuperadas após a TFD e grupo controle foram avaliadas quanto a interferência da TFD na aderência de C. albicans às células do epitélio bucal humano. Em seguida, os animais foram sacrificados e as línguas retiradas para análise macroscópica e histológica. Os biofilmes, línguas e lâminas de aderência foram também analisados por Microscopia Eletrônica de Varredura (MEV). A análise dos dados foi feita por ANOVA e Teste de Tukey, teste t de Student e Kruskal-Wallis (P < 0,05). A TFD aplicada à cultura planctônica de C. albicans foi dose-dependente com redução significativa a partir da menor concentração testada (0,39 μM) e 100% de morte das células a partir de 3,12 μM. A TFD em biofilme formado in vitro reduziu 0,74 log10 de C. albicans com redução de leveduras e hifas...
The aims of the present study were evaluate the antifungal action of Photodynamic Therapy (PDT) on Candida albicans planktonic cultures and biofilms formed in vitro and experimental candidosis model in mice, as well as its interference on C. albicans adherence on humans buccal epithelial cells in vitro. Standard strain of C. albicans (ATCC 18804) was used for assays. Green Light Emitting Diode (LED- 532 ± 10 nm) was used as light source with an output power of 90 mW and erythrosine photosensitizer at concentrations range from 200 to 0.39 μM for the assays on plaktonic cultures. The biofilm was formed on plates of 96- wells and treated with the erythrosine photosensitizer at a concentration of 400 μM and LED light. 56 adults and males mice were immunosuppressed and inoculated with suspensions containing 108 cells/ mL of C. albicans. The animals were submitted to erythrosine dye- mediated PDT (400 μM) and irradiated by LED. Before and after the treatments, yeasts from the animals´ oral cavities were recovered. The yeasts recovered after PDT and control group were evaluated for interference of PDT on C. albicans adherence to humans buccal epithelial cells. Following, the animals were sacrified and their tongues taken away for macroscopic and histological analysis. The biofilms, tongues and adherence slices were also analized by Scanning Electron Microscopy (SEM). The analysis of the dates were done by ANOVA and Tukey test, Student t test and Kruskal-Wallis (P < 0.05). PDT applied on C. albicans planktonic culture was concentrationdependent with significant reduction from minor concentration tested (0.39 μM) and 100% of cells death from 3.12 μM. C. albicans biofilm formed in vitro was reduced 0.74 log10 by PDT with reduction of yeasts and hyphaes verified by SEM. In vivo, 0.73 log10 of C. albicans and 35% adherence on buccal epithelial cells were reduced, but there was not... (Complete abstract click electronic access below)
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Costa, Anna Carolina Borges Pereira da. „Avaliação da terapia fotodinâmica em candida albicans in vitro e in vivo /“. São José dos Campos : [s.n.], 2011. http://hdl.handle.net/11449/95047.
Der volle Inhalt der QuelleAnnotation:
Orientador: Antonio Olavo Cardoso JorgeBanca: Juliana Campos Junqueira
Banca: Aguinaldo Silva Garcez Segundo
Resumo: Os objetivos do presente estudo foram avaliar a ação antifúngica da Terapia Fotodinâmica (TFD) em culturas planctônicas e biofilmes de Candida albicans formados in vitro e em modelo de candidose experimental em camundongos, bem como sua interferência na aderência de C. albicans às células epiteliais bucais humanas in vitro. Foi utilizada cepa padrão de C. albicans (ATCC 18804) para os ensaios. Como fonte de luz foi utilizado o Diodo Emissor de Luz (LED) com emissão de luz verde (532±10 nm) com potência de 90 mW e fotossensibilizador eritrosina nas concentrações variando de 200 a 0,39 μM para os ensaios em cultura plactônica. O biofilme foi formado em placas de 96 poços e tratado com o fotossensibilizador eritrosina na concentração de 400 μM e luz LED. 56 camundongos machos e adultos foram imunossuprimidos e inoculados com suspensões contendo 108 células/mL de C. albicans. Os animais foram submetidos a TFD mediada pelo corante eritrosina (400 μM) e irradiados por LED. Antes e após os tratamentos, foram recuperadas leveduras da cavidade bucal dos animais. As leveduras recuperadas após a TFD e grupo controle foram avaliadas quanto a interferência da TFD na aderência de C. albicans às células do epitélio bucal humano. Em seguida, os animais foram sacrificados e as línguas retiradas para análise macroscópica e histológica. Os biofilmes, línguas e lâminas de aderência foram também analisados por Microscopia Eletrônica de Varredura (MEV). A análise dos dados foi feita por ANOVA e Teste de Tukey, teste t de Student e Kruskal-Wallis (P < 0,05). A TFD aplicada à cultura planctônica de C. albicans foi dose-dependente com redução significativa a partir da menor concentração testada (0,39 μM) e 100% de morte das células a partir de 3,12 μM. A TFD em biofilme formado in vitro reduziu 0,74 log10 de C. albicans com redução de leveduras e hifas... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The aims of the present study were evaluate the antifungal action of Photodynamic Therapy (PDT) on Candida albicans planktonic cultures and biofilms formed in vitro and experimental candidosis model in mice, as well as its interference on C. albicans adherence on humans buccal epithelial cells in vitro. Standard strain of C. albicans (ATCC 18804) was used for assays. Green Light Emitting Diode (LED- 532 ± 10 nm) was used as light source with an output power of 90 mW and erythrosine photosensitizer at concentrations range from 200 to 0.39 μM for the assays on plaktonic cultures. The biofilm was formed on plates of 96- wells and treated with the erythrosine photosensitizer at a concentration of 400 μM and LED light. 56 adults and males mice were immunosuppressed and inoculated with suspensions containing 108 cells/ mL of C. albicans. The animals were submitted to erythrosine dye- mediated PDT (400 μM) and irradiated by LED. Before and after the treatments, yeasts from the animals' oral cavities were recovered. The yeasts recovered after PDT and control group were evaluated for interference of PDT on C. albicans adherence to humans buccal epithelial cells. Following, the animals were sacrified and their tongues taken away for macroscopic and histological analysis. The biofilms, tongues and adherence slices were also analized by Scanning Electron Microscopy (SEM). The analysis of the dates were done by ANOVA and Tukey test, Student t test and Kruskal-Wallis (P < 0.05). PDT applied on C. albicans planktonic culture was concentrationdependent with significant reduction from minor concentration tested (0.39 μM) and 100% of cells death from 3.12 μM. C. albicans biofilm formed in vitro was reduced 0.74 log10 by PDT with reduction of yeasts and hyphaes verified by SEM. In vivo, 0.73 log10 of C. albicans and 35% adherence on buccal epithelial cells were reduced, but there was not... (Complete abstract click electronic access below)
Mestre
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LIMA, FABIANO de. „Efeitos do laser em baixa intensidade em Candida albicans; estudo in vitro de parâmetros da luz“. reponame:Repositório Institucional do IPEN, 2012. http://repositorio.ipen.br:8080/xmlui/handle/123456789/9927.
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Dissertacao (Mestrado Profissionalizante em Lasers em Odontologia)
IPEN/D-MPLO
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP; Faculdade de Odontologia, Universidade de São Paulo, São Paulo
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SUZUKI, LUIS C. „Desenvolvimento de biofilme formado por Candida albicans in vitro para estudo da terapia fotodinamica“. reponame:Repositório Institucional do IPEN, 2009. http://repositorio.ipen.br:8080/xmlui/handle/123456789/9456.
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Dissertacao (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Pereira, Cristiane Aparecida. „Efeitos da terapia fotodinâmica in vitro em biofilmes formados por Candida albicans, Staphylococcus aureus e Streptococcus mutans /“. São José dos Campos : [s.n.], 2009. http://hdl.handle.net/11449/87936.
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Orientador: Antonio Olavo Cardoso JorgeBanca: Aguinaldo Silva Garcez Segundo
Banca: Luciane Dias de Oliveira
Resumo: O objetivo do trabalho foi avaliar o efeito antimicrobiano da terapia fotodinâmica (TFD) em biofilmes formados por C. albicans (GI), S. aureus (GII) e S. mutans (GIII), isolados e em associações (GIV-VII). Os biofilmes foram formados em discos de resina acrílica esterilizados imersos em caldo de infusão cérebro coração com 5% de sacarose, inoculados com a suspensão microbiana e incubados por 5 dias. Após o período de incubação, os discos foram lavados com solução fisiológica esterilizada para remover as células não-aderidas. Foram avaliados os efeitos do fotossensibilizador azul de metileno (AM) na concentração de 0,1 mg/mL por 5 min e laser AsGaAl (660 nm) por 98 s, isolados e em conjunto. Os biofilmes foram desprendidos em solução fisiológica em agitador ultrasônico. Foram realizadas diluições e alíquotas semeadas em ágar seletivos e incubadas por 48 h. Os números de UFC/mL em Log10 foram analisados estatisticamente (ANOVA, teste de Tukey, p< 0.05). Também foi realizada a microscopia eletrônica de varredura (MEV) nos discos com biofilmes dos grupos controle e TFD. Foram observadas reduções significativas na viabilidade de todos os biofilmes expostos ao AM e laser. As reduções (log10) foram maiores em biofilmes isolados, com: 40.22% para C. albicans (GI), 55.91% para S. aureus (GII) e 47.35% para S. mutans (GIII). Nos biofilmes mistos as reduções (log10) foram: 35.08% para C. albicans e 43.9% para S. aureus (GIV); 31.54% para C. albicans e 38.7% para S. mutans (GV); 40.12% para S. aureus e 37.14% para S. mutans (GVI); 19.56% para C. albicans, 28.41% para S. aureus e 24.94% para S. mutans (GVII). As imagens de MEV demonstraram uma fotossensibilização letal predominantemente nas camadas superiores dos biofilmes. Conclui-se que a TFD pode ser uma abordagem útil no controle de biofilmes bucais.
Abstract: The purpose of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) in biofilms formed by C. albicans (GI), S. aureus (GII) and S. mutans (GIII), alone and in associations (GIVGVII). The biofilms were grown in acrylic resin discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension and incubated for 5 days. On the fifth day, the discs were washed in sterile saline solution in order to remove loosely bound material. The effects of the methylene blue (MB) photosensitizers at a concentration of 0,1 mg/mL for 5 min and AsGaAl laser (660 nm) for 98 s, alone and conjugated were evaluated. The dics were placed into tubes with sterile saline solution and sonicated for 30 s in order to disperse the biofilms. Ten-fold serial dilutions were carried out and aliquots plated in selective agar which were then incubated for 48 h. Then the numbers CFU/mL (log10) were counted and analyzed statistically (ANOVA, Tukey's test, p< 0,05) Scanning electron microscopy (SEM) on discs with PDT and control biofilms groups were performed. Significant decreases in the viability of all microorganisms were observed when biofilms were exposed to both MB and laser. Reductions (log10) in single-species biofilms were greater than associated biofilms with: 40.22% for C. albicans (GI), 55.91% for S. aureus (GII) and 47.35% for S. mutans (GIII). In associated biofilms the reductions (Log10) were: 35.08% for C. albicans and 43.9% for S. aureus (GIV); 31.54% for C. albicans and 38.7% for S. mutans (GV); 40.12% for S. aureus and 37.14% for S. mutans (GVI); 19.56% for C. albicans, 28.41% for S. aureus and 24.94% for S. mutans (GVII). Scanning electron microscopy micrographs suggested that lethal photosensitization occurred predominantly in the outermost layers of the biofilms. It was concluded that PDT using MB photosensitizer and laser may be a useful approach in the control of oral biofilms.
Mestre
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Emgård, Per. „External otitis and its treatment. Is a group III steroid without antibiotics sufficient therapy? : experimental and clinical studies /“. Umeå : Klinisk vetenskap, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-607.
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Figueiredo, Lívia Mara Alves [UNESP]. „Efeitos da terapia fotodinâmica na candidose experimental e resposta imunológica no modelo hospedeiro de Galleria mellonella“. Universidade Estadual Paulista (UNESP), 2017. http://hdl.handle.net/11449/150842.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A terapia fotodinâmica (TFD) tem demonstrado ação antimicrobiana sobre as leveduras do gênero Candida, sendo considerada uma técnica promissora para o tratamento de candidose. Recentemente foi relatado que a aplicação de TFD também pode resultar em ativação do sistema imunológico, contribuindo para a melhora da infecção. Assim, o objetivo desse estudo foi avaliar a ação da TFD e da terapia laser sobre a resposta imunológica à candidose experimental utilizando Galleria mellonella como modelo hospedeiro de infecção. Larvas de G. mellonella foram infectadas com diferentes cepas de Candida albicans e, após 30 min, foram tratadas com TFD mediada por azul de metileno e laser diodo emitido em 660 nm. A seguir, as larvas foram incubadas a 37°C por sete dias e analisadas diariamente para determinação da curva de sobrevivência. Para o estudo da resposta imunológica, após os tempos de 3, 6, 18 h da TFD foram realizados testes de determinação da densidade de hemócitos na hemolinfa de G. mellonella. Os dados obtidos na curva de sobrevivência foram avaliados pelo teste de Log-rank (Mantel Cox) e os resultados da análise imunológica por análise de variância ANOVA e teste de Tukey, com significância de 5%. Os resultados obtidos demonstraram que tanto para a cepa ATCC 18804 como para a cepa clínica 17 de C. albicans, a TFD prolongou a sobrevivência das larvas de G. mellonella infectadas por uma dose fúngica letal. Entretanto, houve diferença estatisticamente significante entre os grupos PDT e controle somente com cepa ATCC (p=0,0056). Foi verificado também que a infecção pela cepa ATCC 18804 de C. albicans em G. mellonella levou a uma redução no número de hemócitos na hemolinfa, e o tratamento com TFD e terapia laser conseguiram aumentar o número de hemócitos, mas sem apresentar diferença estatística significante. Foi possível concluir com esse estudo que a TFD bem como a terapia laser influenciaram de maneira positiva na melhora da infecção por C. albicans no modelo de G. mellonella.
Photodynamic therapy (PDT) has demonstrated antimicrobial activity on the yeast of the genus Candida and is considered a promising technique for the treatment of candidiasis. Recently it was reported that the application of PDT may also result in activation of the immune system, contributing to the improvement of the infection. The objective of this study is to evaluate the action of PDT and laser therapy on the immune response to experimental candidiasis using Galleria mellonella as host of the infection. G. mellonella larvae were infected with different Candida albicans strains and, after 30 min were treated with methylene blue-mediated PDT and laser diode emitted at 660 nm. Then, the larvae were incubated at 37° C for seven days and analyzed daily in order to determine the survival curve. For the study of the immunological response, after intervals of 3, 6, 18 h of the PDT, tests were performed to determine the density of hemocytes in the hemolymph of G. mellonella. The data obtained in the survival curve were evaluated by the Log-rank test (Mantel Cox) and the results of the immunological analysis by analysis of variance ANOVA and Tukey test, with significance of 5%. The results demonstrated that for both the ATCC 18804 strain and the C. albicans clinical strain 17, PDT prolonged the survival of the infected G. mellonella larvae by a lethal fungal dose. However, there was a statistically significant difference between the PDT and the control groups only with ATCC strain (p = 0.0056). It was also found that infection by the C. albicans ATCC 18804 strain in G. mellonella led to a reduction in the number of hemocytes in the hemolymph, and the treatment with PDT and laser therapy succeeded in increasing the number of hemocytes but without significant statistical difference . It was possible to conclude from this study that PDT as well as laser therapy positively influenced the improvement of C. albicans infection in the G. mellonella model.
Bücher zum Thema "Candida albicans – diet therapy"
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Terrass, Stephen. Candidiasis: (candida albicans). Madrid: Tutor, 1996.
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Candida albicans et candidose généralisée. Sherbrooke, Québec: Éditions du IIIe millénaire, 1990.
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3
Foundation, Price-Pottenger Nutrition, Hrsg. The Candida albicans yeast-free cookbook. New Canaan, Conn: Keats Pub., 1985.
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4
Jay, Joëlle. Le Candida albicans, un signal d'alarme. Montréal: Santé Action, 1992.
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5
Elizabeth, Simonsen, Hrsg. Candida albicans: Yeast-free recipes for renewed health and vitality. Wellingborough, Northamptonshire: Thorsons, 1989.
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Rockwell, Sally J. Coping with Candida cook book. Seattle: Diet Design by Rockwell, 1986.
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Remington, Jane. The yeast-free kitchen: Recipes to help you achieve victory over the yeast beast, Candida albicans. [Houston, Tex: J. Remington, 1993.
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Hurt, Jones Marjorie, und Crook Cynthia P, Hrsg. The yeast connection cookbook: A guide to good nutrition and better health. Jackson, Tenn: Professional Books, 1989.
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Foundation, Price-Pottenger Nutrition, Hrsg. The Candida Albicans yeast-free cookbook: How good nutrition can help fight the epidemic of yeast-related diseases. 2. Aufl. Los Angeles, CA: Keats Pub, 2000.
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Crook, William G. The Yeast connection cookbook: A guide to good nutrition and better health. Jackson, Tenn: Professional Books, 2001.
Den vollen Inhalt der Quelle findenBuchteile zum Thema "Candida albicans – diet therapy"
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Segal, Esther. „Inhibitors of Candida Albicans Adhesion to Prevent Candidiasis“. In Toward Anti-Adhesion Therapy for Microbial Diseases, 197–206. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0415-9_24.
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Vasile Rusu, Alexandru, Berta Alvarez Penedo, Ann-Kristin Schwarze und Monica Trif. „The Influence of Candida spp. in Intestinal Microbiota; Diet Therapy, the Emerging Conditions Related to Candida in Athletes and Elderly People“. In Candidiasis [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.92791.
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Smith, Stephanie J., und Rohini J. Manuel. „The Biology of Fungi“. In Tutorial Topics in Infection for the Combined Infection Training Programme. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198801740.003.0009.
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Fungi are found ubiquitously in the environment such as soil, water, and food. There are an estimated 1.5 million fungal species worldwide, although this number is felt to be grossly underestimated and is regularly updated. Of these vast numbers, around 500 fungi to date have been implicated in human disease. As opposed to bacteria, which are prokaryotes, fungi are eukaryotes, meaning they have a well-defined nucleus and have membrane- bound organelles in the cytoplasm, including an endoplasmic reticulum and a golgi apparatus. In 1969, the scientist R. H. Whittaker first proposed that organisms be classified into five kingdoms: Monera (Bacteria), Protista (Algae and Protozoans), Plantae (Plants), Mycetae (Fungi), and Animalia (Animals). Since then, there have been dramatic changes to the classifications of fungi, largely due to the appliance of phylogenetic molecular techniques. This has resulted in variances to the number of phylums, and the species assigned to them. Table 3.1 shows the seven phyla of the Fungi Kingdom. The majority of fungi pathogenic to humans inhabit the Ascomycota and Basidiomycota phyla. Fungi used to be dually named if they had a pleomorphic life cycle with sexual/ asexual stages (teleomorph/ anamorph, respectively), which meant that fungi often had two names and were classed differently. This practice was discontinued in January 2013 after the International Commission on the Taxonomy of Fungi decided that a ‘one fungus, one name’ approach should be followed. Fungi can be unicellular (yeast) or multicellular (fungi). Yeasts may look globose in nature when grown, whereas multicellular fungi grow as tubular, filamentous material called hyphae that can create a branching, hyphal network called a mycelium. Hyphae may have septa that cross their walls or be nonseptate, which is a method of differentiating fungi. An early hyphal outgrowth from a spore is called a germ tube. The germ tube test can be used to differentiate the yeasts Candida albicans and Candida dubliniensis from other Candida species. The fungal cell wall is composed of chitin and glucans, which are different components to the human cell wall. This means that they can be an effective target for antifungal therapy.
Konferenzberichte zum Thema "Candida albicans – diet therapy"
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Adjimann, Tamara, Thaila Q. Corrêa, Fernanda Alves und Sebastião Pratavieira. „Evaluation of optical redox ratio in Candida albicans cells exposed to photobiomodulation“. In Mechanisms of Photobiomodulation Therapy XV, herausgegeben von Michael R. Hamblin, James D. Carroll und Praveen Arany. SPIE, 2020. http://dx.doi.org/10.1117/12.2541459.
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Spezzia, Teresita, Susana Alejandra Torres-Hurtado, Julián Ramírez Ramírez, Julio-Cesar Ramírez-San-Juan und Rubén Ramos-García. „Efficient in vitro photodynamic inactivation of Candida albicans by repetitive light doses“. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVII, herausgegeben von David H. Kessel und Tayyaba Hasan. SPIE, 2018. http://dx.doi.org/10.1117/12.2289300.
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