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Zeitschriftenartikel zum Thema "Calliphorid flies"

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Authement, Melissa Lein, Leon G. Higley und William Wyatt Hoback. „Anoxia Tolerance in Four Forensically Important Calliphorid Species“. Forensic Sciences 3, Nr. 1 (27.12.2022): 1–11. http://dx.doi.org/10.3390/forensicsci3010001.

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Forensically important blow flies, Diptera: Calliphoridae, are among the first organisms to colonize carrion. After eggs hatch, the larvae of most blow fly species feed in an aggregation or “mass”. While in this mass larvae may experience periods of hypoxia/anoxia, but the tolerance of blow fly larvae to anoxic conditions is poorly studied. We tested the anoxia tolerance of four species of calliphorids (Calliphora vicina, Cochliomyia macellaria, Lucilia sericata, and Phormia regina), by examining actively feeding third-stage larvae across five temperatures. Experiments were conducted by exposing larvae to pure nitrogen environments and determining mortality at set time intervals. All species show significant linear relationships between survival time and temperature under anoxic conditions. Of species tested, C. macellaria had the greatest tolerance to anoxia (LT50 of 9 h at 20 °C). In contrast, C. vicina was the least tolerant (LT50 of 2.2 h at 40 °C). With all species, survivorship decreased with increasing temperature. Unlike many other insects tested in severe hypoxia, the larvae of the calliphorids tested, which included members of three subfamilies, were not tolerant of anoxic conditions. From these findings, it seems likely that hypoxia is a significant limitation for maggots in a maggot mass, particularly when the mass temperature is high (>40 °C). Forensically, these data provide a limit on potential maggot survival on bodies that have been submerged or otherwise experience severe hypoxia before discovery.
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Mello, Renata da Silva, Margareth M. C. Queiroz und Valéria M. Aguiar-Coelho. „Population fluctuations of calliphorid species (Diptera, Calliphoridae) in the Biological Reserve of Tinguá, state of Rio de Janeiro, Brazil“. Iheringia. Série Zoologia 97, Nr. 4 (Dezember 2007): 481–85. http://dx.doi.org/10.1590/s0073-47212007000400019.

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The purpose of this work was to determine the diversity and population fluctuations of calliphorid flies in the Biological Reserve of Tinguá (ReBio-Tinguá), Nova Iguaçu, state of Rio de Janeiro, Brazil and to correlate their occurrence with the environmental variables of temperature, rainfall and relative air humidity. Specimens of Diptera were collected monthly between June 2002 and January 2005 using four traps placed at four points along a trail and exposed for 48 hours. The traps were baited with sardines and the trapped insects were stored in 70% alcohol. It was collected 8,528 calliphorids, thirteen species were identified among the blowflies including Laneela nigripes Guimarães 1977, Chrysomya megacephala (Fabricius, 1794), C. albiceps (Wiedemann, 1819), C. putoria (Wiedemann, 1830), Chloroprocta idioidea (Robineau-Devoidy, 1830), Cochliomyia macellaria (Fabricius, 1775), Hemilucilia semidiaphana (Rondani, 1850), H. segmentaria (Fabricius, 1805), Lucilia eximia (Wiedemann,1819), L. cuprina (Wiedemann, 1830), Paralucilia pseudolyrcea (Mello, 1969), Mesembrinella sp. and Eumesembrinella pauciseta (Aldrich, 1922). No significant correlation was found between the abundance of blowflies and the temperature and relative air humidity. Only C. megacephala and C. albiceps showed a positive and significant correlation with rainfall. An analysis of grouping by month (UPGMA) revealed no seasonal difference in the composition of the community, indicating that the community of calliphorid flies is probably more influenced by the ecological niches occupied by each species than by the seasons of the year.
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Mello, Renata S., Margareth M. C. Queiroz, André F. Nunes-Freitas und Valéria M. Aguiar-Coelho. „Calliphorid fly (Diptera, Calliphoridae) attraction to different colored traps in the Tingua Biological Reserve, Rio de Janeiro, Brazil“. Iheringia. Série Zoologia 99, Nr. 4 (Dezember 2009): 426–30. http://dx.doi.org/10.1590/s0073-47212009000400013.

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The present study intended to analyze calliphorid attraction to traps painted in a variety of colors and the calliphorid constancy index in the Tingua Biological Reserve, Rio de Janeiro state, Brazil. The Diptera were collected monthly in the Reserve, between 2002 and 2005, totaling 24 samplings. Four traps containing sardines as bait were painted olive green, blood red, black, or white and exposed for 48 h at four equidistant points, 50 m from each other. To determine the calliphorid species constancy, the Bodenheirmer constancy index was used throughout the study. To analyze differences in the total abundance between species and in their color selection, an ANCOVA test with a significance level of 5 % and a Tukey post-test were used, considering the categories species and color as cofactors and climatic variables as co-variables (temperature, relative humidity and precipitation), since the samples were collected over two years. 10,444 insects were captured. Of these, 56 % belonged to the Calliphoridae family, totaling 13 species, with the most frequent species being Laneela nigripes (28.5 %), Hemilucilia semidiaphana (17 %), and Mesembrinella sp. (16.4 %). The other species had frequencies lower than 12 %. Nine species were considered constant, two accessories, and two accidental. The data indicated that the most frequent species presented significant differences between themselves concerning abundance over the captured months, however, the Tukey post-test indicated differences only between a few of them. The black trap presented the higher relative calliphorid frequency (27.34 %), followed by green (25 %), red (24.0 %), and white (23.7 %), although the species abundance in the different colored traps did not differ significantly among themselves. Therefore, there was no Calliphorid flies preference for any of the tested colors.
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Howlett, B. G., M. M. Davidson, D. E. Pattemore, M. K. Walker und W. R. Nelson. „Seasonality of calliphorid and sarcophagid flies across Canterbury arable farms requiring pollinators“. New Zealand Plant Protection 69 (08.01.2016): 290–95. http://dx.doi.org/10.30843/nzpp.2016.69.5899.

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Calliphorid and sarcophagid flies are common widespread pollinators of vegetable and forage seed crops Information regarding their seasonal occurrence on arable farms growing these crops may help predict their temporal effectiveness and flexibility as pollinators Window intercept traps (12 per farm) were placed at the edges of crop paddocks across five arable farms in Canterbury for periods of 16 weeks from 2012 to 2015 Total trapping days for each season over the four years were summer 128 autumn 92 winter 199 and spring 35 Flies from at least six species were commonly caught from spring to autumn Mean farm insect counts found Calliphora vicina to be the most abundant species in spring (2373) summer (891) and winter (32) while Oxysarcodexia varia (2129) and Pollenia spp (1903) were most abundant in autumn Their broad temporal abundance shows their potential to pollinate crops that flower at different times
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PARALUPPI, Norival D., Jurandir C. de VASCONCELOS, Josenilda S. de AQUINO, Eloy G. CASTELLÓN und Maria do S. B. da SILVA. „CALLIPHORIDAE (DIPTERA) IN MANAUS: IV. BACTERIA ISOLATED FROM BLOWFLIES COLLECTED IN STREET MARKETS“. Acta Amazonica 26, Nr. 1-2 (Juni 1996): 93–96. http://dx.doi.org/10.1590/1809-43921996261096.

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Staphylococcus aureus, Escherichia coli, Proteussp., Providenciasp., Citrobactersp. and Klebsiellasp. were isolated from calliphorid flies collected in eight street markets in the city of Manaus, Amazonas State, Brazil. The presence of £. coliin the samples suggests that faecal contamination is occurring and that these flies are potential vehicles of enteropathogenic bacteria to exposed foods.
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KURAHASHI, Hiromu, und V. K. THAPA. „Notes on the Nepalese calliphorid flies (Insecta : Diptera)“. Medical Entomology and Zoology 45, Supplement (1994): 179–252. http://dx.doi.org/10.7601/mez.45.179.

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Schmidlin, Franziska G., Jon J. Sullivan, Mike H. Bowie und Brad G. Howlett. „Insect flower visitors of planted native species within the arable landscape on the Canterbury Plains, New Zealand“. New Zealand Plant Protection 71 (02.07.2018): 198–206. http://dx.doi.org/10.30843/nzpp.2018.71.170.

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Almost all of the original native vegetation of Canterbury Plains has been replaced with an arable landscape of managed exotic vegetation. A previous study planted small areas of native trees on arable farms in 2013 to enhance the abundance and diversity of beneficial insect crop pollinators. The aim of the current study was to assess insect flower visitation at three sites in the fifth year after planting. Weekly standardised surveys of native flower visitors were conducted between September 2017 and February 2018. A total of 2349 insects from 37 taxa were observed within three native plantings. Native bees (Lasioglossum sordidum 20%) and the honey bee, Apis mellifera (19%), were the most common followed by the large hoverfly, Melangyna novaezelandiae (16%). The calliphorid flies, brown blowfly Calliphora stygia (8%) and blue blowfly Calliphora vicina (6%), were also well represented. The most abundant insects visited four or more of the eight study plant species. Most (52%) of the flower visitors where natives. Many of these insects are known crop pollinators and it is likely that they assist with crop pollination.
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Montalva, Cristian, Karin Collier, Christian Luz und Richard A. Humber. „Pandora bullata (Entomophthoromycota: Entomophthorales) affecting calliphorid flies in central Brazil“. Acta Tropica 158 (Juni 2016): 177–80. http://dx.doi.org/10.1016/j.actatropica.2016.03.007.

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Bauer, Amely M., Alexandra Bauer und Jeffery K. Tomberlin. „Effects of Photoperiod on the Development of Forensically Important Blow Fly Chrysomya rufifacies (Diptera: Calliphoridae)“. Journal of Medical Entomology 57, Nr. 5 (18.04.2020): 1382–89. http://dx.doi.org/10.1093/jme/tjaa058.

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Abstract Estimation of the time of colonization (TOC) is often based on laboratory studies that document arthropod development. Precise data for forensically important species, such as blow flies (Diptera: Calliphoridae), are essential for accuracy in the estimate of TOC. Calliphorid development is a quantitative trait and thus depends on a host of variables. In calliphorids, studies showed photoperiod can play a role in development. However, there has been little research to date on the effects of photoperiod, and available data indicate the impact is species-specific. In this study, the effects of photoperiod on the development of Chrysomya rufifacies (Macquart), were examined. Chrysomya rufifacies is a fly of great medical and legal importance and is often encountered on vertebrate remains in temperate and tropic regions throughout the world, including North and Central America, Asia, and Australia. Larvae were reared under light regimes of 12, 16, and 24 h of light at 28.5 ± 0.0°C, 86.2 ± 0.3 RH. Minimum development time for each stage did not differ significantly for the applied photoperiods, nor were there significant differences in total minimum postembryonic development time. Photoperiod did not significantly affect larval size or growth rate. The data suggest that light durations investigated in this study do not influence the development of C. rufifacies. This indicates that photoperiod may not be a concern for forensic entomologists in Texas, United States, or other areas with similar conditions when estimating the TOC for this species. Validation efforts are encouraged to verify this conclusion.
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KURAHASHI, Hiromu, und Qamar BANU. „Notes on the Bangladesh calliphorid flies of medical importance (Insecta : Diptera)“. Medical Entomology and Zoology 40, supplement (1989): 97–111. http://dx.doi.org/10.7601/mez.40.97_2.

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Dissertationen zum Thema "Calliphorid flies"

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Stamper, Trevor I. „Improving the Accuracy of Postmortem Interval Estimations Using Carrion Flies (Diptera: Sarcophagidae, Calliphoridae and Muscidae)“. Cincinnati, Ohio : University of Cincinnati, 2008. http://rave.ohiolink.edu/etdc//view?acc_num=ucin1227108162.

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Thesis (Ph.D.)--University of Cincinnati, 2008.
Advisor: Ronald DeBry (Committee Chair); Theresa Culley (Committee Member); Gregory Dahlem (Committee Member); George Uetz (Committee Member); Anthony Perzigian (Committee Member). Title from electronic thesis title page (viewed Dec. 27, 2009). Keywords: Forensic entomology; sarcophagidae; calliphoridae; nocturnal oviposition. Includes abstract. Includes bibliographical references.
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Yusseff, Sohath Zamira. „Distribution, Dna Barcoding And Phylogenetics Of Caribbean Calliphoridae Flies: Tools For Forensic Studies“. ScholarWorks @ UVM, 2018. https://scholarworks.uvm.edu/graddis/847.

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Blow flies (Diptera: Calliphoridae) are among the most dominant and conspicuous insects in the decomposition process. They are important in forensic entomology to determine time of death and, in certain situations, cause of death or relocation of a body. Insects are now included as standard operating procedures in crime scene investigations in many countries, however, this is not standard procedure in the Caribbean area due to lack of knowledge of insects involved in cadaveric decomposition. Successful application of forensic entomology depends on solid underlying data. Our main goal is to advance the knowledge of Calliphoridae in the Caribbean to enable forensic entomology studies. We performed a mega-transect across the Caribbean and extensively collected flies attracted to rotten meat baits during five years from 2011 to 2015. Overall we collected 61,332 flies of which 34,650 were Calliphoridae. We sampled 16 of the 18 species of forensically important Caribbean Calliphoridae and three continental species. We determine the diversity and distribution of Calliphoridae in the Caribbean. We also present a thorough DNA barcode dataset, covering the geographic range of most species in the region. Finally we established phylogenetic relationships among Calliphoridae species and test biogeographical hypotheses, and patterns of diversification and endemism in the Caribbean. In sum, this is the most comprehensive study of the family Calliphoridae from the Caribbean that will open the door for future research on forensic entomology in the region.
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Lewis, Sarah E. „The Genomics of Development Rate Variation in Cochliomyia macellaria (Diptera| Calliphoridae)“. Thesis, Purdue University, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10841813.

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Development rate is a quantitative trait that displays significant variation within many species, including Cochliomyia macellaria Meigen (Diptera: Calliphoridae). Calliphorids are a family of dipterans known as blow flies and are commonly used in forensic entomology to estimate the minimum postmortem interval (PMIMIN), given some assumptions are made. In order to dissect the genetic underpinnings of development rate variation in this species, artificial selection for fast and slow development with population-based resequencing was used. The objective of this study is to isolate and characterize genomic regions that are correlated to development rate variation in blow flies. The first approach used known regulatory development genes from Drosophila melanogaster Meigen (Diptera: Drosophilidae) and isolated variants that were associated with development time changes in artificially selected fast and slow development C. macellaria strains. Three variants located in Ras and Eip74EF were associated with fast or slow development in selection strains with a significant change in allele frequency. The second approach involved the comparison of pooled artificially selected fast and slow C. macellaria genomes to investigate the genetic basis of development rate variation. When comparing the fast and slow genomes, 699 sequences were identified that contained 7290 variants with consistent changes in allele frequency. The variants indicated that the genomic regions that are associated with development rate were associated with developmental processes, including regulation of RNA polymerase II activity, and transporter activity, such as protein dimerization. Of the 699 sequences, 69 sequences were related to Achaete-scute complex and 14 were related to Cyp12A7. The identification of the genomic regions that associated with development rates from this study provides an important resource for future studies in identifying potential genetic markers to increase the effectiveness of PMIMIN estimates. By using significantly associated variants as a priori candidates for future studies, the data increases the understanding of natural development variation in blow flies.

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GARCIA, CRISALIDA R. „Radiomarcacao de chrysomya megacephala (Fabricius, 1794) (Diptera, Calliphoridae) e criacao de Belonuchus rufipennis (Fabricius, 1801) (Coleoptera, Staphylinidae) em ovos desta mosca“. reponame:Repositório Institucional do IPEN, 1993. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10343.

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Tese (Doutoramento)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
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Kirkpatrick, Ryan Scott. „Nocturnal light and temperature influences on necrophagous, carrion-associating blow fly species (Diptera: Calliphoridae) of forensic importance in Central Texas“. Texas A&M University, 2004. http://hdl.handle.net/1969.1/1104.

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It is commonly thought that blow flies are nocturnally inactive. Blowflies are often important in helping to estimate post mortem intervals (PMI) for corpses found at death scenes. If blow flies oviposit during nocturnal hours, there could possibly be up to 12-hour discrepancies for estimates of PMI. Two blow fly species, Phaenicia eximia (Wiedemann) and Cochliomyia macellaria (F.), oviposited nocturnally on ground beef baits in 2003, during the onset of low-atmospheric pressure (an air mass with lower atmospheric pressure than that of the air surrounding it) at rural study sites near Snook (Burleson County), Texas, under artificial lighting of at least 1500 footcandles when temperatures were at 26?C or higher. Trials conducted under similar conditions during the same year without a low-pressure system moving into the area resulted in no nocturnal blow fly oviposition. Nocturnal blow fly oviposition went undetected once low-atmospheric pressure systems settled into the study area, irrespective of temperature and lighting conditions. Thus, this study illustrates that there is a connection between the onset of low-atmospheric pressure and nocturnal blow fly oviposition on baits under artificial lighting of 1500 footcandles or more when temperatures are 26 ?C or above. After concluding 2003 experiments, statistical analyses supplied evidence that woodland habitat was more favorable for blow fly oviposition than prairieland habitat throughout the 2003 study, irrespective of species or time of year. Furthermore, statistical analyses conducted on nocturnal and diurnal temperature ranges furnished evidence to support the hypothesis that 2003 nocturnal temperatures had more of an effect on daily ovipositing than diurnal temperatures for P. eximia and Cynomyopsis cadaverina (Robineau-Desvoidy).
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Kulenkampff, Kyle Sieghard. „DNA barcoding of forensically important blow flies (Diptera: Calliphoridae) within the Western Cape of South Africa“. Master's thesis, Faculty of Health Sciences, 2019. https://hdl.handle.net/11427/31810.

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In forensic entomology, determining species identity is a crucial step towards estimating post mortem interval. DNA barcoding can aid in the identification of unknown forensically relevant species, and this requires the comparison of DNA barcodes to reference data from known species. However, there is a lack of DNA barcode reference data of forensically relevant Calliphoridae species in the Western Cape (South Africa). DNA barcodes were generated for the COI and ITS2 markers for 41 forensically relevant Calliphoridae specimens, representing seven species from six localities in the Western Cape: Chrysomya albiceps (n = 3), Chrysomya chloropyga (n = 8), Chrysomya marginalis (n = 5), Chrysomya megacephala (n = 7), Hemipyrellia fernandica (n = 1), Lucilia cuprina (n = 8) and Lucilia sericata (n = 9). This data was combined with that from Cooke et al. (2018) (n = 40), and subjected to rigorous statistical and phylogenetic analyses. Phylogenetic analysis which combined data for both COI and ITS2 barcodes returned monophyletic clades for each species with increased support when compared to using each barcode individually. This combined dataset was able to discriminate between L. cuprina and L. sericata with full support (100% pP), which was not achieved previously. DNA barcodes were evaluated for intra- and inter-specific variance as well as haplotype patterning. No haplotype patterning was observed for either barcodes across sampled localities. Lastly, a single-blinded approach was used to assess the dataset, whereby DNA barcodes from ‘unknown’ specimens were correctly identified using this reference data. These identifications were more accurate than those using GenBank® or BOLD, highlighting the importance of using locally relevant reference data. This study has contributed new data pertaining to DNA barcodes for seven Calliphoridae species, which was previously scarce for the Western Cape, and this has directly contributed to an improvement in the accuracy of local species identification.
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Brits, Devon, Margot Brooks und Martin Herrer Villet. „Diversity of bacteria isolated from the flies Musca domestica (Muscidae) andChrysomya megacephala (Calliphoridae) with emphasis on vectored pathogens“. Entomological Society of Southern Africa, 2016. http://hdl.handle.net/10962/66904.

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We evaluated the bacteria occurring externally on Musca domestica and Chrysomya megacephala, the two most common synanthropic flies which may be found at many refuse sites throughout the world. Bacteria cultured from 10 specimens of each species were isolated, Gram-stained and examined microscopically, and divided into morphologically distinct ‘pseudospecies', to avoid excessive duplication of genetic identification. About 350 bp of the 16S ribosomalRNAgene was amplified from genomicDNAextracted from each ‘pseudospecies', sequenced, and bacteria identified using BLASTn. Nineteen different types of colony were identified from M. domestica, with Pseudomonas sp. and Swine Manure Bacterium SP14 being most abundant. Chrysomya megacephala yielded 15 distinct pseudospecies with total colony counts approximating to 10 000 from 10 plates, where 80 % of colonies were non-pathogenic Bacillus pumilus. A total of 18 species were identified genetically: three shared by the fly species; four unique to C. megacephala, and 13 unique to M. domestica. Half of these 18 species were pathogenic, two or three others were food spoilers and the rest were environmental or commensal bacteria from soil or plant matter. This study added three new pathogenic strains of bacteria and one new environmental strain to the list of bacteria reported to be vectored by these flies.
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Dias, Leonice Seolin. „Biodiversidade de moscas Calliphoridae e Muscidae no depósito de lixo urbano de Presidente Prudente, São Paulo, Brasil“. Universidade do Oeste Paulista, 2008. http://bdtd.unoeste.br:8080/tede/handle/tede/323.

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The study of Calliphoridae and Muscidae biodiversity in the garbage dump of Presidente Prudente, São Paulo, Brazil, was carried out between March, 2006 and March, 2007. To that purpose, six traps made of plastic bottles with 2 L capacity, and containing bovine liver as attractive, were placed around the garbage discarding site. The total of Calliphoridae captured was 44,688. Chrysomya megacephala was the most frequent species (93.61%; n= 41,833) of fly, with significant differences (p<0.05) between C. megacephala and C. albiceps (1.50%; n= 672) and C. putoria (0.79%; n= 352). There was influence of temperature and of rainfall on the C. megacephala population. In the coldest months, the capture of flies, especially C. albiceps e de C. putoria was extremely low. Regarding the Muscidae family, 1307 individuals were captured. Musca domestica was the most frequent species (99.6%; n= 1,302). The other 0.4% (n= 5) corresponded to Ophyra aenescens. The highest number of Muscidae was observed in the summer and in the springer. Thus, it is concluded that the garbage site of Presidente Prudente is an environment that gives conditions to maintenance of Calliphoridae and Muscidae, especially C. megacephala.
Estudou-se a biodiversidade de califorídeos e muscídeos no lixão de Presidente Prudente, São Paulo, Brasil, no período de março de 2006 a março de 2007. Para esse propósito, seis armadilhas com garrafas tipo pet de 2 litros, contendo como atrativo fígado bovino, foram alocadas ao redor do lixão. Foram capturadas 44.688 califorídeos, com maior freqüência das espécies Chrysomya megacephala (93,61%; n= 41.833), seguindo-se de C. albiceps (1,5%; n= 672) e C. putoria (0,79%; n= 352), com diferença significativa entre a primeira e as demais espécies (p<0,05). Houve influência da temperatura e da precipitação pluviométrica na sazonalidade de C. megacephala, com maiores capturas nos meses quentes e chuvosos (p<0,05). Nos meses frios, a captura das moscas, especialmente de C. albiceps e de C. putoria foi praticamente ausente. No caso dos muscídeos, capturou-se 1.307 espécimes, sendo que a espécie Musca domestica representou 99,6% (n= 1.302) e a espécie Ophyra aenescens 0,4% (n= 5), havendo maior incidência no verão, seguido da primavera, outono e inverno. Dessa forma, conclui-se que o depósito de lixo urbano de Presidente Prudente oferece condições para a manutenção de moscas varejeiras, especialmente C. megacephala, e de mosca doméstica.
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FERRAZ, Adriana Cristina Pedroso. „Avalia??o de novas dietas e o efeito da adi??o de antibi?ticos no desenvolvimento de Chrysomya albiceps (Wiedemann, 1819) e Chrysomya putoria (Wiedemann, 1830) (Diptera: Calliphoridae)“. Universidade Federal Rural do Rio de Janeiro, 2012. https://tede.ufrrj.br/jspui/handle/jspui/1652.

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The research was divided into chapters. The first assessed the post-embryonic development of Chrysomya albiceps (Widemann) on chicken gizzard (control: beef). There were four replications (100g each diet, 40 1st instar/2nd generation larvae) per treatment, each recipient was placed in a larger one containing sawdust and then sealed. The mature larvae were weighed and stored in test tubes. The variation among mature larva weight means and the duration of the larva, pupa and total stages were analyzed by the Student t test (?=5%), the viabilities by ANOVA and the sex ratio by the chi-square test. The following were recorded: mean temperature 25.6oC and 72.4% relative air humidity, larva-adult period mean duration of 8.1 days (meat) and 8.2 days (gizzard); 71% to 87% larva viability; 100% and 99% pupa viability 58% and 67% larva and adult viabilities, respectively. Chicken gizzard was shown to be satisfactory as diet for C. albiceps. The second chapter assessed the post embryonic development of Chysomya putoria (Widemann) in chicken gizzard and homogenized chicken gizzard in 65% agar (control: meat). Four replications (60 mL diet, 40 1st instar/5th generation larvae) were made per treatment. The homogenate was prepared in a mixer (gizzard, distilled water and agar). A mean temperature of 20.6 oC and 67.7% relative air humidity were recorded. The mean duration of the larva-adult period was 8.868 days (meat), 8.676 days (gizzard) and 9.067 days (homogenate); the larva viability was 98%; 92% and 73%; the pupa viability was 98%; 91% and 71%; the larva and adult viabilities were 93%; 83% and 64%, respectively. There were significant difference in the duration of the pupa period between meat and the homogenate. Both diets were shown to be satisfactory for C. putoria. The third chapter assessed different ciprofloxacin concentrations (3.33 ?g/mL; 6.66 ?g/mL and 13.33 ?g/mL in gizzard/65% agar homogenate) on C. putoria development (the control received distilled water). They were replicated four times (60 grams diet, 40 1st instar/3rd generation larvae) in an acclimatized chamber 30oC day/28 oC night, 70+10%relative air humidity and 14-hour photoperiod. There was no significant difference for: mean individual larva weight, mean duration of the larva inoculation until abandonment and the larva, pupa and total stages. Only treatment 2 differed significantly from the control in the larval and total viability. Ciprofloxacin seemed not to alter C. putoria development. The fourth chapter assessed different gentamicin concentrations (4.44?g/mL; 13.33?g/mL and 66.66?g/mL) on C. putoria. The materials and methods were similar to those of chapter III. There was no significant difference for: mean individual larva weight, mean duration of the larva inoculation until abandonment and the larva, pupa and total stages. Only treatment 2 differed significantly from the control for larva viability. Gentamicin seemed not to alter C. putoria development. The fifth chapter assessed different ampicillin concentrations (66?g/mL; 81.33?g/mL and 166.66?g/mL) on C. putoria. The materials and methods were similar to chapter III. There was no significant difference for: mean individual larva weight, mean duration of the larvae inoculation until abandonment and the larval, pupa and total stages. There was no significant difference for larva and total viability, but pupa viability in T1 differed significantly from the control and T2, and T3 differed from the control. Ampicillin seemed not to alter C. putoria development.
A pesquisa foi dividida em cap?tulos. O primeiro avaliou o desenvolvimento p?s-embrion?rio de Chrysomya albiceps (Widemann) em moela de frango (controle: carne bovina). Foram quatro repeti??es (100g de dieta cada, 40 larvas de 1? instar/2? gera??o) por tratamento, cada recipiente inserido em outro maior contendo serragem e vedado. As larvas maduras foram pesadas e armazenadas em tubos de ensaio. A varia??o entre m?dias de massa de larvas maduras e dura??es dos est?gios de larva, pupa e total foram analisadas por Teste t de Student (?=5%), as viabilidades por ANOVA, a raz?o sexual pelo qui-quadrado. Foram registradas temperatura m?dia 25,6?C e umidade relativa do ar m?dia 72,4%; dura??o m?dia do per?odo de larva a adulto 8,1 dias (carne) e 8,2 (moela); viabilidades de larva 71% e 87%; viabilidades de pupa 100% e 99%; viabilidades de larva a adulto 58% e 67%, respectivamente. Moela de frango se mostrou satisfat?ria como dieta para C. albiceps. O segundo cap?tulo avaliou desenvolvimento p?s-embrion?rio de Chysomya putoria (Widemann) em moela e homogenato de moela de frango em agar 65% (controle: carne). Foram quatro repeti??es (60 mL de dieta, 40 larvas de 1? instar/5?gera??o) por tratamento. O homogenato foi preparado em mixer (moela, ?gua destilada e agar). Foram registradas temperatura m?dia 20,6? C e umidade relativa do ar m?dia 67,7%. A dura??o m?dia do per?odo de larva a adulto foi 8,868 dias (carne), 8,676 (moela) e 9,067 (homogenato); as viabilidades larvais 98%; 92% e 73%; as viabilidades de pupa 98%; 91% e 71%; as viabilidades de larva a adulto 93%; 83% e 64%, respectivamente. Houve diferen?a significativa na dura??o do per?odo pupal entre carne e homogenato. Ambas dietas mostraram-se satisfat?rias para C. putoria. O terceiro cap?tulo avaliou diferentes concentra??es de ciprofloxacino (3,33 ?g/mL; 6,66 ?g/mL e 13,33 ?g/mL em homogenato de moela/agar 65%) sobre desenvolvimento de C. putoria (controle recebeu agua destilada). Foram replicados quatro vezes (60 gramas dieta, 40 larvas 1? ?nstar/3? gera??o) em c?mara climatizada 30?C dia/28?C noite, 70+10% U.R. e 14 horas fotoper?odo. N?o houve diferen?a significativa: massa individual m?dio das larvas, dura??o m?dia da inocula??o das larvas at? abandono e est?gios larval, pupal e total. Apenas tratamento 2 diferiu significativamente do controle nas viabilidades larval e total. Ciprofloxacino pareceu n?o alterar desenvolvimento de C. putoria. O quarto cap?tulo avaliou diferentes concentra??es de gentamicina (4,44?g/mL; 13,33?g/mL e 66,66?g/mL) sobre C. putoria. Os materiais e m?todos foram semelhantes ao do cap?tulo III. N?o houve diferen?a significativa: massa individual m?dia das larvas; dura??o m?dia da inocula??o das larvas at? abandono e dos est?gios larval, pupal e total. Apenas tratamento 2 diferiu significativamente do controle na viabilidade larval. Gentamicina pareceu n?o alterar o desenvolvimento de C. putoria. O quinto cap?tulo avaliou diferentes concentra??es de ampicilina (66?g/mL; 81,33?g/mL e 166,66?g/mL) sobre C. putoria. Os materiais e m?todos foram semelhantes ao cap?tulo III. N?o houve diferen?a significativa: massa individual m?dia das larvas, dura??o m?dia da inocula??o das larvas at? abandono e est?gios larval, pupal e total. N?o houve diferen?a significativa: viabilidades larval e total, por?m viabilidade pupal do T1 diferiu significativamente do controle e T2, e T3 diferiu do controle. Ampicilina pareceu n?o alterar desenvolvimento de C. putoria.
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Chang, Chun-Yen, und 張駿彥. „Taxonomic studies of forensically important blow flies from northern Taiwan (Diptera: Calliphoridae)“. Thesis, 2003. http://ndltd.ncl.edu.tw/handle/76156790312106591762.

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碩士
國立臺灣大學
昆蟲學研究所
91
Forensic entomology is a discipline the uses the arthropod successions and the necrophagous fauna which attracted by corpses, especially the blow flies, to assist the criminal investigation. By analyzing the larvae on the corpses and their life histories, we can estimate postmortem interval (PMI) precisely in hours or to locate the site where the death occurred. Unfortunately, no related study has been preformed before in Taiwan; and furthermore, it’s usually not so easy to identify the blow flies to their species level only using external morphologies. So, it’s important to establish the most basic data to examine and determine those blow fly species. This study uses traps to collect blow flies in northern Taiwan. Results show ten dominant species have been found. According to the morphology of male adults and their genitalia, we successfully identify nine of them. They are Chrysomya megacephala,C. pinguis, C. rufifacies, Lucilia cuprina, L. papuensis, L. porphyrina, Hemipyrellia ligurriens, H. pulchra and Caiusa indica. Male terminalia is the most important character to identify Calliphoridae species, but it’s difficult to recognize female adults and immature stages to species level. Using electronmicrography, we can find the ultra-structures on thoracic micro-chaetotaxy, basal ultra-structure of thoracic seata, ventral processes on 5th and 6th segments of puparium, and plastron of eggs are probably useful in dividing species. These results provided a new, quick and easy way to judge blow fly species. We expect to establish a basic forensic entomology data in Taiwan for further references.
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Bücher zum Thema "Calliphorid flies"

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Kurahashi, H. Blow flies (Insecta: Diptera: Calliphoridae) of the Philippines. [Singapore]: National University of Singapore, 2000.

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Kurahashi, H. Blow flies (Insecta: Diptera: Calliphoridae) of Malaysia and Singapore. [Singapore]: School of Biological Sciences, National University of Singapore, 1997.

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Buchteile zum Thema "Calliphorid flies"

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Agrawal, Sarita, und Shubhra Malviya. „Allozyme Diversity Inforensically Important Indian Species Chrysomya Megacephala (Fabricius) (Diptera: Calliphoridae)“. In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022), 225–34. Dordrecht: Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_22.

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AbstractThe application of electrophoretic technique to study allozyme enzymatic variation has been extensively used to explore hidden genetic variability in natural population and laboratory colonies of many calliphorid flies. Genetic variation at three enzyme loci viz., Alkaline phosphatase (APH), Xanthin dehydrogenase(XDH)and Malate dehydrogenase (MDH) in laboratory colonies of Chrysomya megacephalawere investigated by using polyacrylamide gel electrophoresis (PAGE). In APH three zones of activity were observed. Which have been designated as APH-1, APH-2, and APH-3 in order of increasing anodal migration. The electrophoretic phenotypes with two codominant alleles were observed at APH-3loci. In MDH and XDH only one zone of activity was observed.
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Wisser, A., und W. Nachtigall. „Biomechanical Aspects of the Wing Joints in Flies, Especially in Calliphora erythrocephala“. In Constructional Morphology and Evolution, 193–207. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76156-0_14.

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Whitworth, Terry. „Keys to the Genera and Species of Blow Flies (Diptera: Calliphoridae) of America, North of Mexico *“. In Forensic Entomology, 413–43. Third edition. | Boca Raton, FL : CRC Press, Taylor & Francis Group, [2020]: CRC Press, 2019. http://dx.doi.org/10.4324/9781351163767-20.

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„- Calliphoridae, Oestridae, and Sarcophagidae (Myiasis-Causing Flies)“. In Molecular Detection of Human Parasitic Pathogens, 824–35. CRC Press, 2012. http://dx.doi.org/10.1201/b12264-81.

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Konferenzberichte zum Thema "Calliphorid flies"

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Owings, Charity G. „Mediators of population genetic structure in Indiana blow flies (Diptera: Calliphoridae)“. In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.107639.

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Williams, Kirstin A. „Nocturnal oviposition in forensically important flies (Diptera: Calliphoridae) in South Africa“. In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.111029.

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Haskell, Neal. „Factors affecting diurnal flight and oviposition activity of blow flies (Diptera: Calliphoridae) in Indiana“. In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.110867.

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Tran, Theresa, und Sherah VanLaerhoven. „The Influence of Relative Humidity on the Frequency of Clutches of 3 Forensically Important Blow Flies (Diptera: Calliphoridae)“. In The 1st International Electronic Conference on Entomology. Basel, Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/iece-10380.

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