Auswahl der wissenschaftlichen Literatur zum Thema „C.S.I.R.O. Radiophysics Laboratory“
Geben Sie eine Quelle nach APA, MLA, Chicago, Harvard und anderen Zitierweisen an
Inhaltsverzeichnis
Machen Sie sich mit den Listen der aktuellen Artikel, Bücher, Dissertationen, Berichten und anderer wissenschaftlichen Quellen zum Thema "C.S.I.R.O. Radiophysics Laboratory" bekannt.
Neben jedem Werk im Literaturverzeichnis ist die Option "Zur Bibliographie hinzufügen" verfügbar. Nutzen Sie sie, wird Ihre bibliographische Angabe des gewählten Werkes nach der nötigen Zitierweise (APA, MLA, Harvard, Chicago, Vancouver usw.) automatisch gestaltet.
Sie können auch den vollen Text der wissenschaftlichen Publikation im PDF-Format herunterladen und eine Online-Annotation der Arbeit lesen, wenn die relevanten Parameter in den Metadaten verfügbar sind.
Zeitschriftenartikel zum Thema "C.S.I.R.O. Radiophysics Laboratory"
1
Kamani, J. „Observations on Some Biological Characteristics of Rhipicephalus sanguineus Sensu Lato (Acari: Ixodidae) Under Natural and Laboratory Conditions in Nigeria“. Folia Veterinaria 66, Nr. 1 (01.03.2022): 26–32. http://dx.doi.org/10.2478/fv-2022-0003.
Der volle Inhalt der QuelleAnnotation:
Abstract The brown dog tick, Rhipicephalus sanguineus sensu lato (s. l.) is a globally distributed tick of medical and veterinary significance. This study reports some observations on the biological characteristic of the brown dog ticks kept under natural and laboratory conditions in Nigeria. Variation in biological parameters was observed within the Nigerian R. sanguineus s. l. population kept under various conditions. A higher number of R. sanguineus exhibited the type 1 oviposition pattern than the type 2 pattern. The type 1 pattern was observed in 84.2 % and 80 % of R. sanguineus s. l. reared under laboratory and natural conditions, respectively. The pre-oviposition period was significantly shorter in ticks maintained under laboratory conditions than under natural conditions. Similarly, ticks kept under laboratory conditions laid significantly more eggs with higher egg mass weight than ticks kept under natural conditions. Furthermore, the incubation period and the total weight loss by female ticks throughout the oviposition period differed significantly between the two groups (P < 0.05). The engorged R. sanguineus s. l. females kept at 4 °C survived for up to 21 days and remained viable producing fertile eggs. Egg of R. sanguineus s. l. kept at 4 °C for up to 63 days retain viability and were able to hatch to larvae after variable incubation periods when returned to ambient temperatures depending on the duration of storage at 4 °C. The results showed that the tropical lineage of the brown dog tick can survive and remain fecund at low temperatures, suggesting that it can be translocated and established in temperate regions.
2
N, Rajamurugu, Elangovan S und Sundararaj M. „A Innovative Simple Lab Facility to Teach High Speed Jet Characteristics to Aerospace Engineering Students“. Journal of Engineering Education Transformations 36, Nr. 4 (01.04.2023): 86–93. http://dx.doi.org/10.16920/jeet/2023/v36i4/23118.
Der volle Inhalt der QuelleAnnotation:
Abstract : In a laboratory environment a c o m p r e h e n s i v e d i s c u s s i o n a b o u t t h e j e t characteristics where students can visualize the characteristics, pattern of the jet flow. A project-based learning approach is adopted to make students understanding better about the jet phenomenon. A novel experiment set up is established. Hydraulic analogy concept is adapted in the experimental procedures. A detailed time chart and critical examination of results are presented in this paper. The method of evaluation is presented with results. The discussed learning methodology seems to be very e ff e c t i v e a n d s i g n i f i c a n t l y i n c r e a s e s t h e understanding level. Keywords: Jets, Project Based Learning, Higher Level Competency, Project Study
3
Hue, Olivier. „Prediction of Drafted-Triathlon Race Time From Submaximal Laboratory Testing in Elite Triathletes“. Canadian Journal of Applied Physiology 28, Nr. 4 (01.08.2003): 547–60. http://dx.doi.org/10.1139/h03-042.
Der volle Inhalt der QuelleAnnotation:
Purpose and methods: To determine which physiological variables accurately predict the race time of an Olympic-distance International Triathlon undertaken in drafted conditions, 8 elite triathletes underwent both maximal and submaximal laboratory and field physiological testing: a 400-m maximal swim test; an incremental treadmill test; an incremental cycling test; 30 min of cycling followed by 20 min of running (C-R); and 20 min of control running (R) at the exact same speed variations as in running in C-R. Blood samples were drawn to measure venous lactate concentration after the 400-m swim and the cycle and run segments of C-R. During the maximal cycling and running exercises, data were collected using an automated breath-by-breath system. Results: The only parameters correlated with the overall drafted-triathlon time were lactate concentration noted at the end of the cycle segment (r = 0.83, p < 0. 05) and the distance covered during the running part of the submaximal C-R test (r = -0.92, p < 0.01). Stepwise multiple regression analysis revealed a highly significant (r = 0.96, p < 0.02) relationship between predicted race time (from laboratory measures) and actual race time, using the following calculation: Predicted Triathlon Time (s) = -1.128 (distance covered during R of C-R [m]) + 38.8 ([lactate] at the end of C in C-R) + 13,338. The high R2 value of 0.93 indicated that, taken together, these two laboratory measures could account for 93% of the variance in race times during a drafted triathlon. Conclusion: Complementing previous studies, this study demonstrates that different parameters seem to be reliable for predicting performance in drafted vs. nondrafted Olympic-triathlon races. It also demonstrates that, for elite triathletes competing in a drafted Olympic-distance triathlon, performance is accurately predicted from the results of submaximal laboratory measures. Key words: swimming, cycling, running, lactate, oxygen uptake
4
Pfaller, M. A., S. A. Messer, L. Boyken, H. Huynh, R. J. Hollis und D. J. Diekema. „In Vitro Activities of 5-Fluorocytosine against 8,803 Clinical Isolates of Candida spp.: Global Assessment of Primary Resistance Using National Committee for Clinical Laboratory Standards Susceptibility Testing Methods“. Antimicrobial Agents and Chemotherapy 46, Nr. 11 (November 2002): 3518–21. http://dx.doi.org/10.1128/aac.46.11.3518-3521.2002.
Der volle Inhalt der QuelleAnnotation:
ABSTRACT We determined the in vitro activity of flucytosine (5-fluorocytosine [5FC]) against 8,803 clinical isolates of Candida spp. (18 species) obtained from more than 200 medical centers worldwide between 1992 and 2001. The MICs were determined by broth microdilution tests performed according to NCCLS guidelines by using RPMI 1640 as the test medium and the following interpretive breakpoints: susceptible (S), ≤4 μg/ml; intermediate (I), 8 to 16 μg/ml; resistant (R), ≥32 μg/ml. 5FC was very active against the 8,803 Candida isolates (MIC90, 1 μg/ml), 95% S. A total of 99 to 100% of C. glabrata (MIC90, 0.12 μg/ml), C. parapsilosis (MIC90, 0.25 μg/ml), C. dubliniensis (MIC90, 0.12 μg/ml), C. guilliermondii (MIC90, 0.5 μg/ml), and C. kefyr (MIC90, 1 μg/ml) were susceptible to 5FC at the NCCLS breakpoint. C. albicans (MIC90, 1 μg/ml; 97% S) and C. tropicalis (MIC90, 1 μg/ml; 92% S) were only slightly less susceptible. In contrast, C. krusei was the least susceptible species: 5% S; MIC90, 32 μg/ml. Primary resistance to 5FC is very uncommon among Candida spp. (95% S, 2% I, and 3% R), with the exception of C. krusei (5% S, 67% I, and 28% R). The in vitro activity of 5FC, combined with previous data demonstrating a prolonged post-antifungal effect (2.5 to 4 h) and concentration-independent activity (optimized at 4× MIC), suggest that 5FC could be used in lower doses to reduce host toxicity while maintaining antifungal efficacy.
5
Cherewyk, Jensen E., Taylor J. Grusie-Ogilvie, Sarah E. Parker, Barry R. Blakley und Ahmad N. Al-Dissi. „The Impact of Storage Temperature and Time on Ergot Alkaloid Concentrations“. Toxins 15, Nr. 8 (05.08.2023): 497. http://dx.doi.org/10.3390/toxins15080497.
Der volle Inhalt der QuelleAnnotation:
Ergot sclerotia produce toxic secondary metabolites, ergot alkaloids, that infect cereal crops and grasses. Ergot alkaloids have two isomeric configurations: the C-8-R-isomer (R-epimer), and the C-8-S-isomer (S-epimer). Ergot contaminated matrices, such as cereal grains or grasses, may be stored for extended periods at various temperatures before being analyzed, utilized, or consumed. This study assessed the concentration of six common ergot alkaloids in both configurations found in naturally contaminated wheat over time (one, two, and four months) at different temperatures (room temperature, +4 °C, and −20 °C) using ultra-high-performance liquid chromatography–tandem mass spectrometry. The data indicate that the total ergot concentration within a natural contaminated sample varies over time at room temperature, +4 °C, and −20 °C. The total ergot concentration increased until month two, and decreased at month four, independent of temperature (p < 0.05). The total R-epimer concentration appeared to be less stable over time than the total S-epimer concentration. The changes in the total R and total S-epimer concentrations may have been caused by changes in the ergocristine and ergocristinine concentrations, respectively. Time and temperature should be considered when storing potentially contaminated matrices in a laboratory or practical agriculture situations. Quantification of ergot contaminated matrices should occur prior to their use to ensure the most reliable estimates of the concentration of ergot.
6
Hinkle, Kenneth H., David L. Lambert und Robert F. Wing. „Molecular Bands in the 1.1-1.4 μm Spectra of M-S-C stars“. International Astronomical Union Colloquium 106 (1989): 61. http://dx.doi.org/10.1017/s0252921100062709.
Der volle Inhalt der QuelleAnnotation:
Spectra are presented in the J band (7400 to 9700 cm-1) for four Miras ranging in spectral type from M through C. All the program stars have been observed near minimum light. The program stars cover a considerable range in C/0 and the spectral features exhibit a progression as a function of C/0. The S-type stars contain strong bands not previously reported. Especially striking are two sets of triple-headed bands in the J-band spectrum of the S-type Mira R And. The bandheads, which are degraded to longer wavelengths, are at 7877, 7957, 8030 cm-1 and 8379, 8459, 8530 cm-1. The former triplet, which is the stronger of the two, also is present in the mild S star × Cyg but not in the M star R Cas. Additional heads are found in R And at 7477 cm-1, near the short wavelength edge of strong telluric absorption, and at 8968, 9031, 9063 cm-1. The bands are identified as the Δv = -1, 0, 1, and 2 sequences of a predicted (3II-3Δ) transition of ZrS. Additional conspicuous features in the spectra of × Cyg and R Cas are identified with V0, TiO, and H20 bands. These observations provide additional evidence that ZrS is responsible for the majority of the Keenan-Wing bands in the near infrared (0.7-1.1 μ). With additional laboratory work, the ZrS bands will provide an opportunity to measure sulfur abundances in late-type stellar photospheres.
7
Karromallah, Muhammad Ikfi, Siti Latifah, Tri Widiastuti, Mahardika Putra Purba und Ibrahim Sumardi. „TINGKAT KESUBURAN TANAH PADA AREA REBOISASI ASRI DI KAWASAN TAMAN NASIONAL GUNUNG PALUNG KABUPATEN KETAPANG (Soil Fertility Level in Asri Reboization Area in Gunung Palung National Park Area Ketapang Regency)“. JURNAL HUTAN LESTARI 10, Nr. 2 (05.10.2022): 472. http://dx.doi.org/10.26418/jhl.v10i2.53519.
Der volle Inhalt der QuelleAnnotation:
Gunung Palung National Park (TNGP) is a protected forest area that has a very important ecological function. Deforestation activities that occur in the TNGP area have been overcome by implementing reforestation. However, there has not been a scientific study on the impact of reforestation activities on the value of soil fertility. The aim of the research was to compare soil fertility levels based on the physical and chemical properties of soil in open area land, reforestation land, and secondary forests in Gunung Palung National Park, Ketapang Regency. The research method was held by survey, taking soil samples in the land around the Gunung Palung National Park area, i.e., open area land, reforestation land, and secondary forest in Laman Satong village, Matan District, Ketapang Regency. Soil samples were taken by purposive sampling with techniques of sample random sampling (SRS). Soil analysis was carried out in the Laboratory of Chemistry and Soil Fertility, Faculty of Agriculture, Tanjungpura University. The value of soil fertility was held by analyzing the physical and chemical properties. The result of the research showed that secondary forest has a texture of sandy clay, bulk density 0.4 gr/cm3 (SR), pH 3.71 (SM), C-organic 8.17% (ST), N-total 0.86% (ST), CEC 20.27 (S) dan C/N 7.20 (R). Reforestation land has a sandy clay texture, bulk density 1.24gr/cm3 (R), pH 4.39 (SM), C-organic 2.61% (S), N-total 0.,35% (S), CEC 7.87 (R), C/N 7.40 (R). Open area land has a fairly good soil fertility level with a sandy clay texture, bulk density 0,98gr/cm3 (R), pH 4.31 (SM), C-organic 2.26% (S), N-total 0.32% (S), CEC 7.66 (R) dan C/N 8.65 (R). The conclusion of the research showed that the best soil fertility of the land was on the secondary forest, then follow by reforestation land of ASRI and the lowest in open land area.Keywords: Laman Satong Village, Reforestation Area, Soil FertilityAbstrakTaman Nasional Gunung Palung (TNGP) adalah kawasan hutan lindung yang memiliki fungsi ekologi sangat penting. Kegiatan deforestasi yang terjadi di kawasan TNGP telah dilakukan reboisasi, dan belum dilakukan kajian ilmiah dampak dari kegiatan reboisasi terhadap nilai kesuburan tanah. Penelitian ini bertujuan menganalisis tingkat kesuburan tanah berdasarkan sifat fisik dan kimia tanah pada lahan reboisasi yayasan ASRI. Membandingkan tingkat kesuburan tanah pada lahan reboisasi dengan lahan terbuka, dan hutan sekunder di kawasan Taman Nasional Gunung Palung Kabupaten Ketapang. Metode penelitian dilaksanakan dengan metode survei, pengambilan sampel tanah di Desa Laman Satong, Kecamatan Matan, Kabupaten Ketapang. Pengambilan sampel tanah secara sampel random sampling (SRS). Analisis tanah dilakukan di laboratorium kimia dan kesuburan tanah Fakultas Pertanian UNTAN. Nilai kesuburan tanah dilakukan dengan menganalisis sifat fisik dan sifat kimia tanah dengan kriteria kesuburan tanah. Hutan sekunder memiliki tekstur lempung liat berpasir, bulk density 0,4gr/cm3 (SR), pH 3,71 (SM), C-organik 8,17% (ST), N-total 0,86% (ST), KTK 20,27 (S) dan C/N 7,20 (R). Lahan reboisasi memiliki tekstur lempung berpasir, bulk density 1,24gr/cm3 (R), pH 4,39 (SM), C-organik 2,61% (S), N-total 0,35% (S), KTK 7,87 (R), C/N 7,40 (R) . Lahan terbuka memiliki tingkat kesuburan tanah yang tergolong cukup baik dan memiliki tekstur lempung liat berpasir, bulk density 0,98gr/cm3 (R), pH 4,31 (SM), C-organik 2,26% (S), N-total 0,32% (S), KTK 7,66 (R) dan C/N 8,65 (R). Hasil penelitian menunjukkan tingkat kesuburan tanah terbaik pada lahan hutan sekunder kemudian pada lahan reboisasi ASRI dan yang terendah pada areal lahan terbuka.Kata Kunci: Desa Laman Satong, Area Reboisasi, Kesuburan Tanah,
8
Wu, Xibao, Teng Zhang, Lang Pan, Lingyue Wang, Hongle Xu und Liyao Dong. „Germination Requirements Differ between Fenoxaprop-P-ethyl Resistant and Susceptible Japanese Foxtail (Alopecurus japonicus) Biotypes“. Weed Science 64, Nr. 4 (Dezember 2016): 653–63. http://dx.doi.org/10.1614/ws-d-16-00040.1.
Der volle Inhalt der QuelleAnnotation:
Herbicide-resistant (R biotype) and -sensitive (S biotype) individuals were identified from the same population, and seed was increased for each biotype for three generations. We conducted laboratory experiments to determine the effects of temperature, light, salt stress, osmotic stress, pH, and burial depth on the germination and emergence of resistant and sensitive biotypes of Japanese foxtail. The results revealed that there was no difference in the final germination rate between the two biotypes under different temperature conditions, but time to obtain 50% germination or emergence (tE50) and mean germination time of the R biotype were higher than that of the S biotype at 10 C and 15/10 C 12-h day/night regime. In dark conditions, the final germination rate of the S biotype was higher and lower than that of the R biotype at 10 and 25 C, respectively. The overall germination rate of the R biotype was lower than that in the S biotype, and extended germination time was required in extreme conditions, such as 250 mM NaCl and −0.4 MPa osmotic potential. The change in environmental pH had no effect on the germination of the two biotypes. Emergence of the R biotype was lower than the S biotype when seed was buried at least 8 cm deep in an organic matter substrate. This study demonstrated the pleiotropic effects of a resistance allele on seed germination and emergence under different environmental conditions. Deep tillage could be used to reduce the growth and spread of resistant Japanese foxtail individuals.
9
Kutílek, Štěpán, Martina Vracovská, Kamila Pečenková, Hana Brožíková, Richard Pikner und Zlatka Fejfarková. „Calcemia and Inflammatory Markers in Early-Onset Neonatal Infection“. Acta Medica (Hradec Kralove, Czech Republic) 62, Nr. 2 (2019): 58–61. http://dx.doi.org/10.14712/18059694.2019.47.
Der volle Inhalt der QuelleAnnotation:
Introduction: Ionised hypocalcemia (S-Ca2+) has been repeatedly observed in neonates with sepsis. Our aim was to evaluate total calcemia (S-Ca) and its relationship to laboratory markers of infection. Methods: We retrospectively evaluated total calcemia (S-Ca) and its relationship to laboratory markers of sepsis/infection (serum levels of C-reactive protein – S-CRP and procalcitonin – S-PCT) in 29 full-term neonates with early-onset neonatal infection hospitalized at our neonatology ward between 2012 and 2016. The control group consisted of 705 neonates without infection. Results: In neonates with early-onset infection , the S-Ca on day 1, 2 and 3 was significantly lower (p < 0.0001; p < 0.0001; p = 0.05 versus controls) same as the pooled S-Ca (p < 0.0001 versus controls). There was a weak negative correlation between pooled S-Ca and S-PCT, or pooled S-Ca and S-CRP (r = −0.22, p = 0.06; r = −0.19, p = 0.09). Conclusion: S-Ca was decreased in neonates with early-onset infection and did show a slight tendency to inverse correlation with S-CRP and S-PCT. Pediatricians must be aware of the fact that a drop in total S-Ca should alert their attention to the risk of neonatal infection, and, likewise, that the children with neonatal infection are at a higher risk of hypocalcemia with all its consequences.
10
Nazarov, Serdar, und Bayram Jumayev. „Project-based Laboratory Assignments to Support Digital Transformation of Education in Turkmenistan“. Journal of Engineering Education Transformations 36, Nr. 1 (01.07.2022): 40–48. http://dx.doi.org/10.16920/jeet/2022/v36i1/22135.
Der volle Inhalt der QuelleAnnotation:
Abstract: On 30th of November, 2018, by the Decree of the Esteemed President of Turkmenistan Gurbanguly Berdimuhammedov, there was accepted a Conception of developing digital economy in Turkmenistan in between 2019-2025. Moreover, on 15th of September, 2017, the Conception of developing digital education in Turkmenistan was approved as well. That is why, new, innovative methodologies and digital solutions are continuously being introduced to the educational system of Turkmenistan. Teaching and training undergraduate students, studying in the field of automation and control engineering, is a tough issue. Because, nowadays, for automation engineer, knowing physics, mathematics, theory of automation and electronics is not enough, moreover, they should also know basics of smart systems, if necessary, programming a microcontroller and methods of using necessary sensors when designing smart systems. In this regards, this paper provides useful information about the steps and key points of integrating digital solutions a n d mi c r o c o n t r o l l e r - b a s e d e d u c a t i o n a l methodologies into the undergraduate educational system of Turkmenistan. Moreover, the effect of the proposed method of teaching and appropriateness of the laboratory assignments were also analysed at the end of the course. As a microcontroller board, “Ardunio UNO” was selected as it is easy-to-learn. Keywords: smart systems, engineering courses, “Arduino UNO” board, methodology of achieving course objectives, Bloom's taxonomy
Bücher zum Thema "C.S.I.R.O. Radiophysics Laboratory"
1
G, Bowen E. Textbook of Radar: A Collective Work by the Staff of the Radiophysics Laboratory C. S. I. R. o Australia. Cambridge University Press, 2016.
Den vollen Inhalt der Quelle findenBuchteile zum Thema "C.S.I.R.O. Radiophysics Laboratory"
1
Li, Jie Jack, Chris Limberakis und Derek A. Pflum. „Carbon−Carbon Bond Formation“. In Modern Organic Synthesis in the Laboratory. Oxford University Press, 2008. http://dx.doi.org/10.1093/oso/9780195187984.003.0011.
Der volle Inhalt der QuelleAnnotation:
Reviews: (a) Vicarion, J. L.; Badia, D.; Carillo, L.; Reyes, E.; Etxebarria, J. Curr. Org. Chem. 2005, 9, 219-235. (b) Mahrwald, R. Ed. In Modern Aldol Reactions; Wiley-VCH: Weinheim, 2004; Vol. 1., pp. 1-335 (c) Mahrwald, R. Ed. In Modern Aldol Reactions; Wiley-VCH: Weinheim, 2004; Vol. 2., pp. 1-345.(d) Machajewski, T. D.; Wong, C.-H. Angew. Chem. Int. Ed. 2000, 39, 1352-1375. (e) Carriera, E. M. In Modern Carbonyl Chemistry; Otera, J.; Wiley-VCH: Weinheim, 2000; Chapter 8: Aldol Reaction: Methodology and Stereochemistry, 227-248. (f) Paterson, I.; Cowden, C. J.; Wallace, D. J. In Modern Carbonyl Chemistry; Otera, J.; Wiley-VCH: Weinheim, 2000; Chapter 9: Stereoselective Aldol Reactions in the Synthesis of Polyketide Natural Products, pp. 249-298. (g) Franklin, A. S.; Paterson, I. Contemp. Org. Synth. 1994, 1 317-338. (h) Heathcock, C. H. In Asymmetric Synthesis; Morrison, J. D., Ed.; Academic Press: Orlando, Fl.; 1984; Vol. 3., Chapter 2: The Aldol Addition Reaction, pp. 111-212. (i) Mukaiyama, T. Org. React. 1982, 28, 203-331. Since the early 1980s, aldol condensations involving boron enolates have gain great importance in asymmetric synthesis, particularly the synthesis of natural products with adjacent stereogenic centers bearing hydroxyl and methyl groups. (Z)-Boron enolates tend to give a high diastereoslectivity preference for the syn-stereochemistry while (E)-boron enolates favor the anti-stereochemistry. Because the B-O and B-C bonds are shorter than other metals with oxygen and carbon, the six membered Zimmerman–Traxler transition state in the aldol condensation tends to be more compact which accentuates steric interactions, thus leading to higher diastereoselectivity. When this feature is coupled with a boron enolate bearing a chiral auxillary, high enantioselectivity is achieved. Boron enolates are generated from a ketone and boron triflate in the presence of an organic base such as triethylamine. Reviews: (a) Abiko, A. Acc. Chem. Res. 2004, 37, 387-395. (b) Cowden, C. J. Org. React. 1997, 51, 1-200.
2
„George, J. and Wilcox, L. 150 Kleinman, S. 88; and Copp, M.A. 14, Glaser, B.G. and Strauss, A.L. 149 117, 128 Goffman, E. 106 Kosofsky Sedgwick, E. 96 Graham, J. and Bowling, B. 64–5 Kox, W. et al. 151 groups: identification of 66–7; legitimation of 200; older 66–7; as laboratory setting: and clerical staff potentially ‘dangerous’ 64–5; racial 78–80; and eating 75–6; Health and 19; sampling/researching unfamiliar Safety in 74–5, 77, 78, 79–80; and 66–8; solidarity of 27; younger 66; illness 76, 77–8; invisible danger in see also dangerous groups 80–1; organisation of 74; and Guadalajara (Mexico) 182, 184, 185, perception of danger in 76–7; 189–91 sharing of work in 76; smells in 75–6 Lancaster, R. 133, 135, 136 Hagell, A. and Newburn, T. 65 Lawrinson, S. and Harris, J. 61 Hearn, J. 107 Le Bon, G. 151 Heidenshohn, F. 33 Lee, A.M. 149 Hobbs, D. 33, 58 Lee, R.M. 17, 28, 67, 72, 116, 181, Hochschild, A.R. 89, 101, 115, 128 182, 184, 197 Hockey, J. 28 Lee-Treweek, G. 114, 116 Holdaway, S. 27, 31 Letherby, G. 92, 99; and Zdrodowski, Holliday, R. et al. 103 D. 99–100 Homan, R. 1, 15, 17, 19, 199 Local Education Authorities (LEAs) hooks, b. 96 171 Howell, N. 69 Local Politics of Race project 170–2, Humphreys, L. 199 178 Hurd, T.L. and McIntyre, A. 95 Lofland, J. and Lofland, L. 149 Ignatiev, N. 173 Lovatt, A.: and O’Conner, J. 44; and Iles, T. 96 Purkis, J. 56 Luhrmann, T.M. 152 James, N. 101, 102, 115, 128 Lukes, S. 73 James, P. 11 Jipson, A. and Becker, P. 161 McCarthy, J. and Zald, M. 151 Jones, S. 33 Mack, M. 11 Jorgensen, D.L. 150 Mackenzie, C. 32 MacLean, N. 150 Karp, D. and Yoels, W. 16 McMahon, M. 96, 109 Katz, W. 150 McRobbie, A. 101 Katz Rothman, B. 106 McVicar, J. 58 Kelly, L. et al. 95 March, R. 143 King, M. and Hunt, R. 149 Mariátegui, J.C. 141“. In Danger in the Field, 218. Routledge, 2002. http://dx.doi.org/10.4324/9780203136119-39.
Der volle Inhalt der QuelleKonferenzberichte zum Thema "C.S.I.R.O. Radiophysics Laboratory"
1
Farag, A. M., J. M. Brown, R. J. Sachs und E. F. Mammen. „PROTHROMBIN TIMES AND CLOTTABLE FIBRINOGEN DETERMINATIONS ON AN AUTOMATED COAGULATION LABORATORY (ACL 810) ANALYZER.“ In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643255.
Der volle Inhalt der QuelleAnnotation:
The ACL 810 is a fully automated, microcomputer controlled centrifugal analyzer (Instrumentation Laboratory/IL). It performs clot based and chromogenic substrate assays. The clot based assay utilizes laser nephelometry. Prothrombin times (PT) in normal and abnormal plasmas were analyzed on the ACL using different thromboplastins and the data compared with a Fibrometer and a Coag-A-Mate. The correlation between the ACL (II reagents) and the Coag-A-Mate and Fibrometer (Thromboplastin C, Dade) for 100 samples were as follows: ACL vs Coag-A-Mate r = 0.87 (p < 0.001), m (slope) 1.13; ACL vs Fibrometer r = 0.92 (p < 0.001), m = 1.1.Since the ACL 810 determines PT and fibrinogen simultaneously, we compared fibrinogen levels on the ACL 810 (II Thromboplastin) with those analyzed on a Multistat III Centrifugal Analyzer (MCA) (bovine thrombin, Hemostasis Laboratories) and a Fibrometer (Data-Fi, Dade). The following correlations were obtained: ACL vs Fibrometer n = 50, r = 0.922 (p < 0.001), m 0.72; ACL vs MCA n = 100, r 0.899 (p < 0.001), m 0.7; MCA vs Fibrometer n = 70, r = 0.954 (p < 0.001), m = 0.936. We also compared different manufacturers' thromboplastins on the ACL 810 in the determination of fibrinogen with the following results:The run-to-run coefficient of variation (CV) for fibrinogen was 4.4% and for PT 0.26%; the intra-run CV was 4.66% and 0.41%. These data reveal excellent correlations for both PT and fibrinogen not only with different instruments but also with different thromboplastins on the same instrument.
2
Fabbrini, N., J. M. Walenga, D. Hoppensteadt und J. Fareed. „LABORATORY EVALUATION OF A MULTICHANNEL VERTICAL PHOTOMETRIC ANALYZER FOR TEE TESTING OF CLOT, CHROMOGENIC AND ELISA BASED METHODS FOR HEMOSTATIC SYSTEM“. In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644605.
Der volle Inhalt der QuelleAnnotation:
Due to major developments in the molecular understanding of the hemostatic system, many newer tests to evaluate bleeding and clotting disorders have been developed. These newer tests are based on clotting, enzymatic and immunologic techniques. Since routine coagulation laboratories are not equipped with instruments capable of enzymatic or immunologic measurements, adaptation of these newer tests to the clinical laboratory has been rather limited. As these tests have a significant impact on the basic diagnostics of hemostatic disorders and the monitoring of antithrombotic therapy, the need for instruments capable of performing each of these tests is evident. We have evaluated a new multiprobe instrument, the FP-910 Coagulation Analyzer (Lab-Systems, Helsinki, Finland), for numerous clot-based, immunologic and amidolytic assays. This instrument has a programmable microprocessor controlled analyzer, mixer and incubator in a semi-automated system capable of hight throughput due to multiple processing. A unique vertical light path system allows quantification of even particulate reactions. It is compatible with most commercially available reagents for clot-based synthetic substrate and ELISA methodologies. Individual methods are preprogrammed in the instrument and additional methods can be programmed for all detection modes. For the clot-based assays (P.T, APTT, Heptest®) , excellent correlations were obtained with the BBL Fibrometer and General Diagnostics Coagamate X2 (n=50, r>0.95). The chromogenic assay kits from various suppliers (AT III, heparin, plasminogen, a-antiplasmin) also correlar-ted well (n=50, r>0.95). Similarly the ELISA based protein C assay also correlated well with the Dynatech® system (n=50, r>0.98). The instrument is easy to operate, has a high throughput in all modes (50-100/hr.), high reproducibility and is economically feasible for routine laboratories involved in multi-parametric testing of hemostasis.
3
Karasek, Oliwia, und Yusuf Arslanparcasi. „Conceptualizing an Academic Teaching and Learning Laboratory for Systems Engineering“. In 14th International Conference on Applied Human Factors and Ergonomics (AHFE 2023). AHFE International, 2023. http://dx.doi.org/10.54941/ahfe1003159.
Der volle Inhalt der QuelleAnnotation:
In an increasingly complex world of goods and services, as well as in the context of digitalization and Industry 4.0, previous product development for intelligent technical systems is reaching its limits. Due to shorter product life cycles, increasing complexity and a growing number of product variants, along with growing demands on the part of stakeholders, etc., new approaches to development are required (Czaja et al., 2017; Haberfellner et al., 2021).However, the use of new development approaches, such as advanced systems engineering (ASE), is accompanied by far-reaching changes for the organization and all people involved. ASE combines methods for system consideration and for requirements management with AI-influenced engineering processes in which all relevant information – from the business idea to market success – is integrated into the development process across disciplines in order to efficiently design the development of complex cyber-physical systems (Dumitrescu et al., 2021).Such new development approaches require a redesign of work organization, as well as versatile competencies and skillsets going beyond classical engineering. Advanced soft skills and new work competencies such as communication, teamwork and agile work methods become more important for example through the introduction of flat hierarchies or cross-functional work structures. Thus, new competencies and qualifications for future engineers are necessary for product development. Against this backdrop, the expansion of targeted competency management and higher education in the engineering sciences must adequately take these developments into account. This is because successful adaptation and transformation of companies in value creation is largely determined by their ability to build up the right competencies as well as to use existing competencies of employees and to develop them in a targeted manner (North et al., 2018).In this paper we outline the required competencies for engineers in the context of advanced systems engineering and derive a novel empirical competency model. Based on this empirical model, possibilities for the conceptualization of competency measures through the framework of constructive alignment at the academic level are presented. Works Cited:Czaja, A., Amon, M., Dumitrescu, R., Lampert, R., & Gaarman, D. (2017). Bedarfsgerechter SE Prozess für einen mittelständischen Hersteller von Automatisierungskomponenten. In S.-O. Schulze, C. Tschirner, R. Kaffenberger & S. Ackva (eds.), Tag des Systems Engineering. Paderborn, 8. -10. November 2017 (pp. 3-12). Carl Hanser Verlag GmbH & Co. KG.Dumitrescu, R., Riedel, O., Gausemeier, J., Albers, A., & Stark, R. (2021). Advanced Systems Engineering Wertschöpfung im Wandel: Engineering in Deutschland - Status quo in Wirtschaft und Wissenschaft. Fraunhofer IEM.Haberfellner, R., de Weck, O., Fricke, E., & Vössner, S. (2021). Systems engineering. Fundamentals and Applications. Springer Nature Switzerland. https://doi.org/10.1007/978-3-030-13431-0North, K., Reinhardt, K., & Sieber-Suter, B. (2018). Kompetenzmanagement in der Praxis. Springer Fachmedien Wiesbaden. https://doi.org/10.1007/978-3-658-16872-8
4
Meschengieser, S., A. Blanco, N. Maugeri, J. Fernandez, M. de Tezanos Pinto und M. Lazzari. „PLATELET CONTENTS OF VON WILLEBRAND FACTOR ANTIGEN (vWF:Ag) AND FIBRINOGEN (I) AND PLATELET FUNCTION (PF) IN MYELODYSPLASTIC SYNDROMS (MS)“. In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644883.
Der volle Inhalt der QuelleAnnotation:
Bleeding in MS is usually associated with thrombocytopenia, but may occur in patients (P) with a normal platelet count (PC) and defects in the release (R) reaction and the arachidonic acid (AA) pathway. Weinvestigated the PF and contents of vWF:Ag and I in 6 P with diagnosis of: Acquired Idiopathic Sideroblastic Anemia (2), Refractory Anemia (2) and Refractory Anemia with Excess of Blasts (2). Laboratory testsincluded: PC; bleeding time (BT)(Ivy); platelet retention to glass beads (PR); platelet aggregation (PA)and R induced by ADP(2.5juM), epinephrine (E)(0.1mM), collagen (C) (l-8ug/ml), AA(0.8-lmM) and A23187 (A)(19jjM); spontaneous PA (SPA); generation (G) and response (Re) to thromboxane A2 like material (TXA2);vWF:Ag and I (electroimmunoassay)in platelet lysates. Results: PC were normal in 6/6, BT were prolonged in 3/6, PR were reduced in 4/6 , PA induced by E were absent in 4/6 and abnormal in the other 2, absenceof secondary PA induced by ADP were observed in 4/6, PA induced by C and AA were abnormal in 5/6. No SPA were observed in 6/6. ATP R were absent with ADP andE in 4/6, with C and AA in 5/6 and abnormal with A in 2/6. The G of TXA2 were absent or decreased and the Re to_TXA2 were normal in all the P. vWF:Ag (X: 0.088U/109Cells) and I (X: 16.8jμg/109cells) were de-creased in_6/6 P and the mean values were lower (p<0.001) than controls (X: 0.284U/109cells and X: 61.2jug/109cells respectively). The PF abnormalities observed are similar to those described by other authors, but the decrease of vWF:Ag and I have not been previously reported.These low levels could be the resultof platelet activation or megacaryocyte dys function.The re was no evidence of platelet hyperactivity inour P. So, we assume that defective megacaryocytopoiesis with an alteration in the storage of the granules contents could be responsible for the depletion observed.
5
Lonsdale, Cameron, und Steven Dedmon. „Fatigue Testing of Microalloyed AAR Class C Wheel Steel“. In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-13366.
Der volle Inhalt der QuelleAnnotation:
Railroad wheels guide a freight car along the rails while supporting mechanical loads, and also serve as the brake drum in the air brake system of a freight car. Since a 36-inch diameter freight car wheel experiences approximately 560 revolutions per mile, and since many North American freight cars accrue 100,000 miles per year in service, fatigue properties of steel are very important. Further, elevated tread temperatures resulting from tread braking are known to significantly reduce the yield strength of the wheel steel at the tread surface. This paper describes fatigue testing of AAR rim quenched Class C wheel steel manufactured with microalloy additions. Small amounts of selected alloy elements were purposely added to develop a wheel steel with improved high temperature yield strength. Rotating bending fatigue tests, conducted at a well-known professional testing laboratory, were performed at ambient and elevated temperatures using complete stress reversal (R = -1) cycling. Stress-life (S-N) curves were constructed and the microalloy steel results were compared to existing fatigue data, and to results for typical Class C steel with no microalloy additions. Past research work is briefly reviewed. Test results are discussed with emphasis on the implications for service performance of wheel steel.
6
Iijima, Takashi, Hirotoshi Enoki, Junichiro Yamabe und Bai An. „Effect of High Pressure Gaseous Hydrogen on Fatigue Properties of SUS304 and SUS316 Austenitic Stainless Steel“. In ASME 2018 Pressure Vessels and Piping Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/pvp2018-84267.
Der volle Inhalt der QuelleAnnotation:
A high pressure material testing system (max. pressure: 140 MPa, temperature range: −80 ∼ 90 °C) was developed to investigate the testing method of material compatibility for high pressure gaseous hydrogen. In this study, SSRT and fatigue life test of JIS SUS304 and SUS316 austenitic stainless steel were performed in high pressure gaseous hydrogen at room temperature, −45, and −80 °C. These testing results were compared with those in laboratory air atmosphere at the same test temperature range. The SSRT tests were performed at a strain rate of 5 × 10−5 s−1 in 105 MPa hydrogen gas, and nominal stress-strain curves were obtained. The 0.2% offset yield strength (Ys) did not show remarkable difference between in hydrogen gas and in laboratory air atmosphere for SUS304 and SUS316. Total elongation after fracture (El) in hydrogen gas at −45 and −80 °C were approximately 15 % for SUS304 and 20% for SUS316. In the case of fatigue life tests, a smooth surface round bar test specimen with a diameter of 7 mm was used at a frequency of 1, 0.1, and 0.01 Hz under stress rate of R = −1 (tension-compression) in 100 MPa hydrogen gas. It can be seen that the fatigue life test results of SUS304 and SUS316 showed same tendency. The fatigue limit at room temperature in 100 MPa hydrogen gas was comparable with that in laboratory air. The room temperature fatigue life in high pressure hydrogen gas appeared to be the more severe condition compared to the fatigue life at low temperature. The normalized stress amplitude (σa / Ts) at the fatigue limit was 0.37 to 0.39 for SUS304 and SUS316 austenitic stainless steels, respectively.
7
Le Delliou, Patrick, und Samuel Geniaut. „Tests on SENT and CT Specimens to Study Geometry Effects in the Brittle to Ductile Transition“. In ASME 2016 Pressure Vessels and Piping Conference. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/pvp2016-63235.
Der volle Inhalt der QuelleAnnotation:
The accurate prediction of ductile fracture behaviour plays an important role in structural integrity assessments of critical engineering structures under fully plastic regime, including nuclear reactors and piping systems. Many structural steels and aluminium alloys generally exhibit significant increases in fracture toughness, characterized by the J-integral, over the first few mm of stable crack extension (Δa), often accompanied by large increases in background plastic deformation. Conventional testing programs to measure crack growth resistance (J–Δa) curves employ three-point bend, SEN(B), or compact, CT. However, laboratory testing of fracture specimens to measure resistance curves (J–Δa) consistently reveals a marked effect of absolute specimen size, geometry, relative crack size (a/W ratio) and loading mode (tension vs. bending) on R-curves. These effects observed in R-curves have enormous practical implications in defect assessments and repair decisions of in-service structures under low constraint conditions. Structural components falling into this category include pressurized piping systems with surface flaws that form during fabrication or during in-service operation. A research program was launched by EDF R&D to study geometry effects (e.g. triaxiality effects) in the brittle to ductile transition of carbon-manganese steels using Single-edge notch tension (SENT) specimens, by comparing the results obtained on these specimens with the results obtained on CT specimens. This paper presents the results of the tests conducted between −40°C and −100°C on a large number of specimens of both types. The toughness values of the SENT specimens appear to be included in the scatter of the CT12.5 ones, so the geometry effect between CT and SENT specimens in the brittle to ductile region is not significant. Moreover, the results of the CT12.5 cut in the L-S direction are not very different of those of the specimens cut in the T-S direction. The Master Curve methodology fits rather well the CT12.5 results, whereas the SENT results are not well covered by this methodology. The energetic approach called GP has been applied to the analysis of some tests. This approach shows that the geometry effect between both types of specimens is limited, in agreement with the experimental observations.
8
Schutz, Ronald W., Gabriel Rombado, David A. Baker, Heath W. Walker und Christopher S. Caldwell. „Qualification Testing of Titanium Stress Joints Designed for Galvanic Hydrogen Mitigation Conveying Hot, Sour Well Fluids“. In ASME 2018 37th International Conference on Ocean, Offshore and Arctic Engineering. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/omae2018-78105.
Der volle Inhalt der QuelleAnnotation:
A three-phase laboratory test qualification program for Titanium Stress Joint (TSJ) use in an offshore steel catenary riser (SCR) system handling hot, marginally sour well brine fluid offers guidance for expanded, safe TSJ use in hot sour well service. Phase 1 results indicated a reasonable concern and risk for long-term excessive hydrogen uptake and damage to the TSJ when directly coupled with the steel riser and steel topside piping. An alternative TSJ design, incorporating an Alloy 625 cathode buffer transition between adjoining steel tubulars, was proposed for mitigation of galvanic hydrogen charging uptake and damage prevention for hot sour fluid service. This “Tri-Metal couple” design was modeled in Phase 2 using polarization curves as input, and simulations projected insignificant hydrogen charging on TSJ bore surfaces exposed to “Worst-Case Sour” brine fluid at 250°F (121°C). Phase 3 aimed at qualifying the TSJ welds for even more severe and acidic sour well fluid service conditions up to 275°F (135°C), via fatigue crack growth rate (FCGR) and J-R fracture toughness testing of weld metal, and S-N fatigue and slow strain rate (SSR) tensile testing of cross-welds. These tests confirmed the high degree of hot sour environmental resistance for Grade 29 Titanium welded joints, and reasonable compatibility with TSJ design requirements.
9
Zhang, L., H. Xu, J. Wang und H. Jiang. „Research on the Mechanism of Nano-Displacement Agent to Water Blocking Damage of Tight Sandstone Gas Reservoir“. In 57th U.S. Rock Mechanics/Geomechanics Symposium. ARMA, 2023. http://dx.doi.org/10.56952/arma-2023-0416.
Der volle Inhalt der QuelleAnnotation:
ABSTRACT Exploring ways to enhance the liquid-phase seepage ability and increase the flow-back rate of external fluids in the production process is an important way to solve the problem of water blocking in tight reservoirs. Based on the analysis of the characteristics of water phase retention damage caused by foreign fluid invasion during the development of tight sandstone gas reservoirs, a set of ether nano-displacement agent system was optimized. The mechanism of wetting modification is revealed from the perspective of the physical composition of nano-displacement agent, and the mechanism of action between nano-displacement agent and rock and different clay minerals is clarified. Laboratory experiments show that :(1) 0.5 wt% CNDAD1# nano-displacement agent fluid can change the wettability of sandstone to gas-phase wetting, "air-salt water-rock" three-phase static contact angle increased from 38.5° to 126°. (2) SEM and EDX results prove that the nano-displacement agent has good macroscopic static and dynamic adsorption performance on the sandstone surface. This paper provides a reference method for exploring the use of environmentally friendly chemical agents to remove water blocking damage in tight sandstone gas reservoirs. INTRODUCTION Tight sandstone gas reservoirs refer to sandstone gas reservoirs with overburden matrix permeability less than or equal to 0.1 mD. Single wells generally have no natural productivity or natural productivity is lower than the lower limit of industrial gas flow. Industrial natural gas production needs to be obtained through fracturing, horizontal wells, multi-branch wells, etc. (Wei G, Zhang F, Li J, et al., 2016; Jia C, Zheng M and Zhang Y, 2012; Zou N, Zhu R, Wu S, et al., 2012). According to the data, China contains a wealth of tight sandstone gas reservoirs, tight sandstone gas resources account for about 27.5 % of China ‘s natural gas resources, mainly distributed in different basins in China, such as Sichuan, Tarim Basin and other regions, and most of the reserves have not been effectively developed (Wang J, Hu Y, Liu Y, et al., 2019; Fuhai J, Tang D, Xu H, et al., 2012; Bybee K, 2007). Therefore, the development of tight sandstone gas reservoirs will become one of the key sources of future energy security in China, and it is also the focus of unconventional oil and gas development. However, most of the tight sandstone gas reservoirs have low natural productivity of single wells. The combination of horizontal well drilling and fracturing is one of the main ways to realize the commercial exploitation of tight sandstone gas reservoirs. However, during the fracturing process, the fracturing fluid flowback is not timely or the edge and bottom water invades the reservoir during the production process. The reservoir will produce a large amount of external water phase retention or edge and bottom water invasion water, forming a ‘ liquid phase trap ‘ and further tight sandstone gas well productivity (Dutta R, Lee C H, Odumabo S, et al., 2012; You Q, Wang H, Zhang Y, et al., 2018; Zhang L, Zhou F, Mou J Y, et al., 2018).
10
Makarov, A. F., Y. V. Tkachuk und A. Yu Shishkov. „ARTIFICIAL HYPOBIOSIS AS A METHOD OF G-FORCE NEGATIVE IMPACT REDUCTION“. In The 17th «OCCUPATION and HEALTH» Russian National Congress with International Participation (OHRNC-2023). FSBSI «IRIOH», 2023. http://dx.doi.org/10.31089/978-5-6042929-1-4-2023-1-291-295.
Der volle Inhalt der QuelleAnnotation:
Dynamic gravitational acceleration, known as a G-force, is an extreme factor for some professions. G-force negative impact realizes in central nervous system adequate functioning disturbance, resulting from oxygen starvation of tissues due to hemodynamic disorders. Known methods of G-force protection are aimed at increasing the maximum short-term G-force value, but not its total safe duration. In a number of studies, in order to increase the maximum value of the tolerated G-force, laboratory animals were lead in to a state of artificial hypobiosis. Mortality decrease was noted with an increase the maximum G-force value. Data about the manifestations of disorders of the central nervous system and the recovery time, as well as changes in the safe G-force duration has not been detected. The study purpose to assess the change in the safe G-force duration while artificial hypobiosis. Materials and methods. Syrian male hamsters weighing 95.5±0.5 g (M±m) were used. The animals were divided into groups — experimental and control. Intramuscular injections of a suspension of α-methyldopa were performed to induce artificial hypobiosis in animals of the experimental group. The control group received of 0.9% NaCl. Dynamic gravitational acceleration modeling was carried out using a centrifuge (r=0.62 m). The animals of both groups were conscious, with positive G-forces pointing downward (head-pelvis). Within 10 seconds (s), the centrifuge rotation speed was increased till the chosen G-force value, G: 30 (angular velocity (ω)=21.79 rad/s), 40 (ω=25.16 rad/s) or 70 (ω=33.28 rad/s). G-force exposure set time, s: 20, 50, 80, 110 or 140. After a complete stop for 10 seconds, the general condition of the animals, injuries, hemorrhages, breathing, heart contractions were assessed. The surviving animals were monitored, consciousness was assessed, coordination disorders were determined according to the following criteria: animal’s pose, productive movement, unsteady gait, circle movement, tilting; Also, the time of restoration of coordination was determined. The animals were observed during the following days to assess the daily survival rate. Necropsy was performed on all non-surviving animals. Results. Survival in the control group: 30 G: 80 s — 5/6, 40 G: 20 s — 6/6, 50 s — 6/6, 80 s — 3/6, 70 G: 20 s — 6/6, 50 s — 4/6, 80 s — 0/10. Survival in the experimental group: 70 G: 50 s — 6/6, 80 s — 10/10, 110 s — 10/10, 140 s — 2/6. After acceleration of 70 G 50 s in animals of the experimental group, coordination disorders were weakly expressed, the coordination recovery time was 1.8±0.3 s, in animals of the control group, violations were assessed as significant, the recovery time was 4.5±0.3 s, which is 2.5 times (p<0.01) more. Next day: tremor persisted in 3 animals of the control group 40 G 80 c. In the remaining animals of all groups, no coordination disorders were found. No external injuries were observed in any animal. No organ or skeletal damage was found during necropsy. Conclusions. An increase of 5.5 times in the time of safe stay of animals in a state of artificial hypobiosis under dynamic overloads has been proven. There was a 2.5‑fold decrease in the coordination recovery time in animals in a state of artificial hypobiosis.
Berichte der Organisationen zum Thema "C.S.I.R.O. Radiophysics Laboratory"
1
Morrison, Mark, und Joshuah Miron. Molecular-Based Analysis of Cellulose Binding Proteins Involved with Adherence to Cellulose by Ruminococcus albus. United States Department of Agriculture, November 2000. http://dx.doi.org/10.32747/2000.7695844.bard.
Der volle Inhalt der QuelleAnnotation:
At the beginning of this project, it was clear that R. albus adhered tightly to cellulose and its efficient degradation of this polysaccharide was dependent on micromolar concentrations of phenylacetic acid (PAA) and phenylpropionic acid (PPA). The objectives for our research were: i) to identify how many different kinds of cellulose binding proteins are produced by Ruminococcus albus; ii) to isolate and clone the genes encoding some of these proteins from the same bacterium; iii) to determine where these various proteins were located and; iv) quantify the relative importance of these proteins in affecting the rate and extent to which the bacterium becomes attached to cellulose. BARD support has facilitated a number of breakthroughs relevant to our fundamental understanding of the adhesion process. First, R. albus possesses multiple mechanisms for adhesion to cellulose. The P.I.'s laboratory has discovered a novel cellulose-binding protein (CbpC) that belongs to the Pil-protein family, and in particular, the type 4 fimbrial proteins. We have also obtained genetic and biochemical evidence demonstrating that, in addition to CbpC-mediated adhesion, R. albus also produces a cellulosome-like complex for adhesion. These breakthroughs resulted from the isolation (in Israel and the US) of spontaneously arising mutants of R. albus strains SY3 and 8, which were completely or partially defective in adhesion to cellulose, respectively. While the SY3 mutant strain was incapable of growth with cellulose as the sole carbon source, the strain 8 mutants showed varying abilities to degrade and grow with cellulose. Biochemical and gene cloning experiments have been used in Israel and the US, respectively, to identify what are believed to be key components of a cellulosome. This combination of cellulose adhesion mechanisms has not been identified previously in any bacterium. Second, differential display, reverse transcription polymerase chain reaction (DD RT-PCR) has been developed for use with R. albus. A major limitation to cellulose research has been the intractability of cellulolytic bacteria to genetic manipulation by techniques such as transposon mutagenesis and gene displacement. The P.I.'s successfully developed DD RT- PCR, which expanded the scope of our research beyond the original objectives of the project, and a subset of the transcripts conditionally expressed in response to PAA and PPA have been identified and characterized. Third, proteins immunochemically related to the CbpC protein of R. albus 8 are present in other R. albus strains and F. intestinalis, Western immunoblots have been used to examine additional strains of R. albus, as well as other cellulolytic bacteria of ruminant origin, for production of proteins immunochemically related to the CbpC protein. The results of these experiments showed that R. albus strains SY3, 7 and B199 all possess a protein of ~25 kDa which cross-reacts with polyclonal anti-CbpC antiserum. Several strains of Butyrivibrio fibrisolvens, Ruminococcus flavefaciens strains C- 94 and FD-1, and Fibrobacter succinogenes S85 produced no proteins that cross-react with the same antiserum. Surprisingly though, F. intestinalis strain DR7 does possess a protein(s) of relatively large molecular mass (~200 kDa) that was strongly cross-reactive with the anti- CbpC antiserum. Scientifically, our studies have helped expand the scope of our fundamental understanding of adhesion mechanisms in cellulose-degrading bacteria, and validated the use of RNA-based techniques to examine physiological responses in bacteria that are nor amenable to genetic manipulations. Because efficient fiber hydrolysis by many anaerobic bacteria requires both tight adhesion to substrate and a stable cellulosome, we believe our findings are also the first step in providing the resources needed to achieve our long-term goal of increasing fiber digestibility in animals.
2
Thomashow, Linda, Leonid Chernin, Ilan Chet, David M. Weller und Dmitri Mavrodi. Genetically Engineered Microbial Agents for Biocontrol of Plant Fungal Diseases. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696521.bard.
Der volle Inhalt der QuelleAnnotation:
The objectives of the project were: a) to construct the site-specific integrative expression cassettes carrying: (i) the chiA gene for a 58-kDa endochitinase, (ii) the pyrrolnitrin biosynthesis operon, and (iii) the acdS gene encoding ACC deaminase; b) to employ these constructs to engineer stable recombinant strains with an expanded repertoire of beneficial activities; c) to evaluate the rhizosphere competence and antifungal activity of the WT and modified strains against pathogenic fungi under laboratory and greenhouse conditions; and d) to monitor the persistence and impact of the introduced strains on culturable and nonculturable rhizosphere microbial populations in the greenhouse and the field. The research generally support our concepts that combining strategically selected genes conferring diverse modes of action against plant pathogens into one organism can improve the efficacy of biological control agents. We hypothesized that biocontrol agents (BCAs) engineered to expand their repertoire of beneficial activities will more effectively control soilborne plant pathogens. In this work, we demonstrated that biocontrol activity of Pseudomonas fluorescens Q8r1-96 and Q2-87, both producing the antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) effective against the plant pathogenic fungus Rhizoctonia solani, can be improved significantly by introducing and expressing either the 1.6-kb gene chiA, encoding the 58-kDa endochitinase ChiA from the rhizosphere strain SerratiaplymuthicaIC1270, or the 5.8-kb prnABCDoperon encoding the broad-range antibiotic pyrrolnitrin (Prn) from another rhizosphere strain, P. fluorescens Pf-5. The PₜₐcchiAandPₜₐcprnABCDcassettes were cloned into the integrative pBK-miniTn7-ΩGm plasmid, and inserted into the genomic DNA of the recipient bacteria. Recombinant derivatives of strains Q8r1-96 and Q2-87 expressing the PₜₐcchiA or PₜₐcprnABCD cassettes produced endochitinase ChiA, or Prn, respectively, in addition to 2,4-DAPG, and the recombinants gave significantly better biocontrol of R. solani on beans under greenhouse conditions. The disease reduction index increased in comparison to the parental strains Q8r1-96 and Q2-87 to 17.5 and 39.0% from 3.2 and 12.4%, respectively, in the case of derivatives carrying the PₜₐcchiAcassette and to 63.1 and 70% vs. 2.8 and 12,4%, respectively, in the case of derivatives carrying the PₜₐcprnABCDcassette. The genetically modified strains exhibited persistence and non-target effects comparable to those of the parental strains in greenhouse soil. Three integrative cassettes carrying the acdS gene encoding ACC deaminase cloned under the control of different promoters were constructed and tested for enhancement of plant growth promotion by biocontrol strains of P. fluorescens and S. plymuthica. The integrative cassettes constructed in this work are already being used as a simple and efficient tool to improve biocontrol activity of various PGPR bacteria against fungi containing chitin in the cell walls or highly sensitive to Prn. Some parts of the work (e. g., construction of integrative cassettes) was collaborative while other parts e.g., (enzyme and antibiotic activity analyses) were fully synergistic. The US partners isolated and provided to the Israeli collaborators the original biocontrol strains P. fluorescens strains Q8r1-96 and Q2-87 and their mutants deficient in 2,4-DAPG production, which were used to evaluate the relative importance of introduction of Prn, chitinase or ACC deaminase genes for improvement of the biocontrol activity of the parental strains. The recombinant strains obtained at HUJI were supplied to the US collaborators for further analysis.