Auswahl der wissenschaftlichen Literatur zum Thema „Botryosphaeria dieback“

Asmalarda gövde hastalıkları (AGH) arasında, Esca sendromu (Phaeomoniella chlamydospora ve Phaeoacremonium oleophilum, Botryosphaeria spp., Eutypa lata, Phomopsis viticola, Cylindrocarpon spp.), Petri hastalığı (Phaeomoniella chlamydospora, Phaeoacremonium spp., Cadophora luteo-olivacea ve Pleurostoma richardsiae), Siyah ayak (Dactylonectria, Ilyonectria, Campylocarpon, Cylindrocladiella veya Neonectria), Botryosphaeriae dieback (Lasiodiplodia theobromae, Neofusicoccum parvum ve Botryosphaeria dothidea) Eutypa dieback, (Eutypa lata ve Diatrypaceae spp.) Phomopsis dieback, (Phomopsis viticola) yer almaktadır. AGH enfeksiyonları kısa veya uzun vadede asmaların ölümüne neden olur. Global ölçekte, bağcılığın sürdürülebilirliğini etkidiğinden ve tüm bağcı ülkelerde hızla yayıldığından, son otuz yıldır asmanın en yıkıcı hastalıkları olarak kabul edilmektedir. AGH’nın kontrolünde sıcak su uygulaması (SSU), tarım kimyasalları ve dezenfektanlar kullanılmaktadır. SSU′na genç asmalar, dinlenme halindeki aşı gözü veya anaç çelikleri, köklü veya aşılı köklü asma fidanları ve Vitis vinifera çeşitleri farklı düzeyde hassasiyet gösterebilirler. Bu hassasiyet çeliklerin veya fidanların büyüdüğü mevsim sıcaklıklarından etkilenebilir ve uygulanacak sıcaklık aralığı, kontrol edilecek patojenlere göre değişebilir. Asma çeliklerindeki AGH′nı baskılamak için çeşidine göre değişen aralıklarda SSU′ları (30-45 dakika süreyle 45-54°C) önerilmektedir. SSU, asma çoğaltma materyalleri ile taşınabilecek filoksera, nematod ve fitoplazma patojenleri için etkin bir kontrol yöntemidir. Kaliteli bir asma fidanı, sağlıklı, ismine doğru, iyi bir bitki formuna sahip, iyi kaynaşmış, virüsler ve patojenlerden arındırılmış, çevresel strese maruz kalmamış olmalıdır. Bu derlemede, asma çoğaltma materyallerinin patojenik bakteri, mantar, nematod, fitoplazma ve filoksera enfeksiyonlarının kontrolünde kullanılan SSU çalışmalarının bir özeti sunulmuştur.

The grapevine trunk diseases Eutypa and Botryosphaeria dieback threaten the sustainability of vineyards worldwide. This study aimed to develop practical and efficient wound protection strategies, which will lead to widespread adoption of preventative control for these diseases and increased longevity of vineyards. Five fungicides (tebuconazole, carbendazim, fluazinam, mancozeb, and flusilazole) were evaluated for efficacy against Eutypa lata and Neofusicoccum luteum infection on pruning wounds of ‘Sauvignon blanc’ in a New Zealand vineyard. All five fungicides controlled infection by both pathogens to varying degrees, with mean percent disease control of 70 to 90% for E. lata and 33 to 93% for N. luteum. This has led to the first known registration of a fungicide, fluazinam (Gem), for simultaneous control of both Eutypa and Botryosphaeria dieback. Furthermore, application of carbendazim with tractor-driven sprayers provided similar control of E. lata and N. luteum to that when applied by hand with a paintbrush. This constitutes the first report of Botryosphaeria dieback control with fungicides applied with tractor-driven sprayers.

In recent years, the number of apple trees affected by Botryosphaeria cankers and dieback has considerably increased in central Chile. This study aimed to identify the species of Botryosphaeriaceae associated with canker and dieback symptoms, estimate disease incidence and distributions, and study their pathogenicity and virulence on apple and other fruit crops. A field survey of 34 commercial orchards of apple (7 to 30 years old) was conducted in 16 localities, obtaining 270 symptomatic branch and trunk samples in 2017 and 2018 growing seasons. The incidence of Botryosphaeria canker and dieback ranged between 5 and 40%, and a total of 255 isolates of Botryosphaeriaceae spp. were obtained from 238 cankers. Morphological identification along with phylogenetic studies of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rDNA, part of the translation elongation factor 1-α (tef1-α), and part of the β-tubulin (tub2) genes allowed us to identify Diplodia mutila (n = 49 isolates), D. seriata (n = 136 isolates), Lasiodiplodia theobromae (n = 16 isolates), and Neofusicoccum arbuti (n = 54 isolates). L. theobromae was isolated mainly from apple dieback from northern localities. All pathogens tested were pathogenic, causing canker and dieback symptoms on lignified twigs of apple, pear, walnut, and green grapevine shoots in the field. Isolates of N. arbuti were the most virulent, reproducing more severe cankers on the lignified tissues inoculated. This study reports, for the first time, D. mutila and L. theobromae associated with Botryosphaeria canker and dieback in Chile, and it is the first description of N. arbuti causing apple dieback worldwide.

Fungi were isolated in pure cultures from decaying giant sequoias in Geneva (Switzerland). Isolates were genetically identified by ITS rDNA sequencing. Young giant sequoia trees were artificially infected with a pure culture of Botryosphaeria parva. Henle-Koch’s Postulates demonstrated that Botryosphaeria parva was pathogenic to Sequoiadendron giganteum. When analysing the microorganisms associated to canker and dieback symptoms in a giant sequoias (Sequoiadendron giganteum) in Geneva, the fungus Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips, teleomorph Botryosphaeria parva (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips, was isolated, whereas such symptoms are commonly associated to Fusicoccum aesculi (teleomorph Botryosphaeria dothidea). These two fungal species belong to the same genus Botryosphaeria of the Botryosphaeriaceae family. Because Neofusicoccum parvum was causing cankers and diebacks in other woody species around the world, we extended the analysis to other trees displaying sequoia dieback symptoms in order to evaluate the involvement of Neofusicoccum parvum in such increasing symptoms in sequoias in Geneva. Dried twigs, trunk, and branch cankers from diseased trees were sampled on several distinct sites. From all samples, isolated fungi in pure cultures showed a phenotype typical of Botryosphaeriaceae species. Isolates were then genetically identified at the species level. Subsequently Neofusicoccum parvum was inoculated to young giant sequoia trees, re-isolated in pure culture from provoked symptoms, and re-identified to fulfil Henle-Koch’s postulates. The identification confirmed that Neofusicoccum parvum was present on all sites, while Fusicoccum aesculi was retrieved only once alone. The inoculation of Neofusicoccum parvum isolates on young sequoias demonstrated for the first time that this fungus was able to develop cankers in Sequoiadendron giganteum. Neofusicoccum parvum could, therefore, be the major cause for dying of giant sequoias in the Geneva Lake area.

Diplodia seriata, one of the major causal agents of Botryosphaeria dieback, spreads worldwide, causing cankers, leaf spots and fruit black rot in grapevine. Vitis rupestris is an American wild grapevine widely used for resistance and rootstock breeding and was found to be highly resistant to Botryosphaeria dieback. The defense responses of V. rupestris to D. seriata 98.1 were analyzed by RNA-seq in this study. There were 1365 differentially expressed genes (DEGs) annotated with Gene Ontology (GO) and enriched by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The DEGs could be allocated to the flavonoid biosynthesis pathway and the plant–pathogen interaction pathway. Among them, 53 DEGs were transcription factors (TFs). The expression levels of 12 genes were further verified by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The aggregation of proteins on the plasma membrane, formation variations in the cytoskeleton and plasmodesmata and hormone regulations revealed a declined physiological status in V. rupestris suspension cells after incubation with the culture filtrates of D. seriata 98.1. This study provides insights into the molecular mechanisms in grapevine cells’ response to D. seriata 98.1, which will be valuable for the control of Botryosphaeria dieback.

Worldwide 11 species of Botryosphaeria have been isolated from grapevines in association with a wide range of decline and dieback symptoms Currently there is little knowledge on what species are present in New Zealand A comprehensive survey was carried out during June to December 2007 to collect Botryosphaeria species from New Zealand vineyards More than 450 isolates including B parva B lutea B australis B stevensii B obtusa and B iberica were isolated from symptomatic material No correlation was observed between the species of Botryosphaeria and grapevine variety or age The highest incidence of Botryosphaeria species (977 ) was from Blenheim and lowest (164 ) was from Otago The most commonly isolated species was B parva followed by B stevensii Fusicoccum type Botryosphaeria species such as B parva and B lutea were more prevalent in the North Island and Diplodia type species including B stevensii and B obtusa dominated in the South Island It is likely that the Botryosphaeria species distribution in New Zealand is influenced by climatic conditions This information has implications for development of control strategies

Botryosphaeria dieback is a fungal grapevine trunk disease that represents a threat for viticulture worldwide due to the decreased production of affected plants and their premature death. This dieback is characterized by a typical wood discoloration called brown stripe. Herein, a proteome comparison of the brown striped wood from Botryosphaeria dieback-affected standing vines cultivars Chardonnay, Gewurztraminer, and Mourvèdre was performed. The transcript analysis for 15 targeted genes and the quantification of both total phenolics and specific stilbenes were also performed. Several pathogenesis-related proteins and members of the antioxidant system were more abundant in the brown striped wood of the three cultivars, whereas other defense-related proteins were less abundant. Additionally, total phenolics and some specific stilbenes were more accumulated in the brown striped wood. Strongest differences among the cultivars concerned proteins of the primary metabolism, which looked to be particularly impaired in the brown striped wood of ‘Chardonnay’. Low abundance of some proteins involved in defense response probably contributes to make global response insufficient to avoid the symptom development. The differential susceptibility of the three grapevine cultivars could be linked to the diverse expression of various proteins involved in defense response, stress tolerance, and metabolism.

Grapevine trunk diseases cause severe damage in grapevines. Management strategies focus on protection of grapevine pruning wounds using chemical fungicides or biological control agents. Botryosphaeria dieback, caused mainly by Lasiodiplodia spp., is one of the main trunk diseases in northwest Mexico. This study obtained endophytic bacteria and fungi from the heritage grapevine Vitis vinifera cv. ‘Mission’ for potential biological control of Botryosphaeria dieback. A collection of 135 bacterial and 37 fungal isolates were obtained and initially tested for antagonistic activity against Lasiodiplodia brasiliensis. The most promising isolates belonging to Trichoderma and Bacillus spp. were selected and characterized to determine their modes of action. Bacillus isolates produced volatile organic compounds that inhibited growth of Neofusicoccum parvum, and diffusible organic compounds with antifungal effects against L. brasiliensis and N. parvum. Trichoderma isolates produced diffusible organic compounds and were mycoparasites. In greenhouse assays, plants inoculated with three Trichoderma asperellum isolates (T20BCMX, EF09BCMX, and EF11BCMX), B. amyloliquefaciens (BEVP26BCMX) or Bacillus sp. (rbES015), applied preventively in soil, gave up to 50% smaller necrotic lesions when compared with the plants inoculated only with L. brasiliensis. In the field, plants inoculated with three Bacillus isolates (BEVP02BCMX, BEVP26BCMX, BEVP31BCMX) or five Trichoderma (T11BCMX, T15BCMX, T17BCMX, T20BCMX, and EF11BCMX) had lesions up to four times smaller than control plants inoculated only with L. brasiliensis. This study has demonstrated the potential of heritage grapevines to provide biological control agents for Botryosphaeria dieback.

Grapevine trunk diseases threaten the longevity of vineyard production in New Zealand This paper provides a summary of the knowledge of the most common fungal grapevine trunk diseases as well as identifying gaps that require further research Current knowledge of symptoms causal organisms etiology and disease control of botryosphaeria dieback esca eutypa dieback and Petri disease are discussed Additional information about how these diseases may be influenced by different vineyard practices common in New Zealand viticulture is provided

Botryosphaeria spp. recently have been identified as important grapevine pathogens worldwide. To date, Botryosphaeria rhodina has been the only species associated with cankers on Vitis vinifera in California. A field survey of 166 vineyards in 21 counties was conducted in order to determine the occurrence of other Botryosphaeria spp. in California. In all, 1,735 samples of cankered trunks, cordons, and spurs were collected. Botryosphaeria spp. were the most common fungi isolated from grapevine cankers in California. Morphological identification along with phylogenetic analysis of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the nuclear ribosomal DNA (rDNA) and a partial sequence of the β-tubulin gene showed that at least seven Botryosphaeria spp. occur on grapevines in California: B. australis, B. dothidea, B. lutea, B. obtusa, B. parva, B. rhodina, and B. stevensii. Botryosphaeria spp. were found in grapevine cankers in all grape-growing regions surveyed in California, whereas incidence and distribution varied with location. Grapevine cankers in California have been associated mainly with Eutypa dieback. However, the frequent recovery of Botryosphaeria spp. from cankers in this study indicates that the role of these fungi in grapevine health needs to be more carefully considered.

La viticulture est constamment sous la menace de maladies, notamment fongiques. Au cours des dernières décennies, les maladies du bois de la vigne (MDB), des pathologies très destructives, menaçant la pérennité du vignoble, sont en pleine expansion. L'une des MDB les plus répandues dans le monde est le dépérissement à Botryosphaeriacées. Les champignons de cette famille utilisent de nombreuses armes biochimiques, y compris des enzymes dégradant les parois cellulaires des plantes (PCWDEs) et des métabolites secondaires phytotoxiques, pour coloniser la vigne. Nous souhaitions produire, purifier et caractériser des PCWDEs et des phytotoxines des Botryosphaeriaceae afin de mieux connaître les mécanismes qui sous-tendent leur pathogénicité et leur virulence. Nous cherchions également à tirer profit des Botryosphaeriaceae pour obtenir de nouvelles enzymes lignocellulolytiques qui pourraient être utiles au bioraffinage végétal. En utilisant des comparaisons génomiques, nous avons mis en évidence la richesse des enzymes actives sur les glucides (CAZymes), potentiellement impliquées dans la dégradation des parois cellulaires des plantes, dans le génome de Neofusicoccum parvum. Lorsqu'il est cultivé dans des fermentations en milieu liquide, N. parvum peut produire de nombreuses CAZymes et métabolites secondaires, notamment dans des milieux de croissance contenant des biomasses lignocellulosiques. Par ailleurs, nous avons établi une méthode de purification de la phytotoxine (R)-melléine en utilisant une approche innovante via la chromatographie de partage centrifuge. Enfin, nous avons également débuté une approche visant la production hétérologue de trois nouvelles CAZymes : une acétylxylane estérase, une pectate lyase et une cellulase encore non caractérisée. Dans l'ensemble, nos résultats ont permis de mieux comprendre l'influence de la biomasse lignocellulosique sur l'expression des facteurs de pathogénicité et de virulence chez N. parvum. Cette étude pourrait également être utile pour découvrir des enzymes plus efficaces pour des applications industrielles
Viticulture is constantly threatened by plant diseases, especially those caused by fungi. In the last decades, Grapevine Trunk Diseases (GTDs) have arisen as highly destructive and rapidly expanding pathologies. One of the most worldwide prevalent GTDs is Botryosphaeria dieback, caused by Botryosphaeriaceae. These fungal species may rely on many biochemical weapons, including Plant Cell Wall-Degrading Enzymes (PCWDEs) and phytotoxic secondary metabolites, to successfully colonize the grapevine. Here, we aimed to produce, purify, and characterize PCWDEs and phytotoxins from Botryosphaeriaceae to increase knowledge of their mechanisms underlying pathogenicity and virulence. We were also interested in taking advantage of Botryosphaeriaceae pathogens to obtain novel lignocellulolytic enzymes that may benefit plant biorefining. Using genomic comparisons, we highlighted the remarkable richness of Carbohydrate-Active Enzymes (CAZymes), potentially involved in the plant cell wall degradation, in the genome of Neofusicoccum parvum. When cultured in submerged fermentations, N. parvum could produce numerous CAZymes and secondary metabolites, notably in growth media containing lignocellulosic biomasses. Furthermore, we established a purification method for the virulence-factor-candidate phytotoxin (R)-mellein using an innovative approach via Centrifugal Partition Chromatography. We also started an approach to heterologously produce three novel CAZymes: an acetylxylan esterase, a pectate lyase, and an uncharacterized cellulase. Overall, our results provided insights into the influence of lignocellulosic biomass in the expression of pathogenicity and virulence factors in N. parvum. This study may also be helpful for the discovery of more efficient enzymes for industrial applications