Auswahl der wissenschaftlichen Literatur zum Thema „Biofilms – Lutte contre“
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Zeitschriftenartikel zum Thema "Biofilms – Lutte contre"
Dalmat, Yann-Mickael. „La firme qui lutte contre les biofilms à Sars-CoV-2… et autres“. Option/Bio 32, Nr. 633-634 (Mai 2021): 13. http://dx.doi.org/10.1016/s0992-5945(21)00104-5.
Der volle Inhalt der QuelleMaldiney, Thomas, Valentin Pineau, Romain Bregigeon, Catherine Neuwirth, Florian Perraud, Bianca Podac, Mathieu Blot et al. „Faire la lumière sur le biofilm en soins critiques : un compartiment mésestimé pour lutter contre les infections associées aux dispositifs médicaux invasifs ?“ Médecine Intensive Réanimation 33, Nr. 2 (07.06.2024): 191–204. http://dx.doi.org/10.37051/mir-00207.
Der volle Inhalt der QuelleKara Terki, I., H. Hassaine, A. Kara Terki, B. Nadira, N. Kara Terki, S. Bellifa, I. Mhamedi und M. Lachachi. „Effects of certain disinfectants and antibiotics on biofilm formation by Staphylococcus aureus isolated from medical devices at the University Hospital Center of Sidi Bel Abbes, Algeria“. African Journal of Clinical and Experimental Microbiology 21, Nr. 4 (25.08.2020): 304–10. http://dx.doi.org/10.4314/ajcem.v21i4.6.
Der volle Inhalt der QuelleBoyer, A., B. Clouzeau, H. N. Bui, F. Vargas, G. Hilbert und D. Gruson. „Nouvelles techniques pour lutter contre le biofilm de la sonde d’intubation“. Réanimation 22, Nr. 3 (Mai 2013): 250–56. http://dx.doi.org/10.1007/s13546-013-0689-4.
Der volle Inhalt der QuelleMion, Sonia, Benjamin Rémy, Laure Plener, Éric Chabrière und David Daudé. „Quorum sensing et quorum quenching : Comment bloquer la communication des bactéries pour inhiber leur virulence ?“ médecine/sciences 35, Nr. 1 (Januar 2019): 31–38. http://dx.doi.org/10.1051/medsci/2018310.
Der volle Inhalt der QuelleBaillif, S., L. Kodjikian, E. Casoli, K. Marion, C. Roques, G. Pellon, D. Hartmann, J. Freney und C. Burillon. „339 Mieux comprendre pour mieux lutter contre l’adhésion bactérienne : creation d’un modèle dynamique d’étude de la formation des biofilms staphylococciques sur les implants intraoculaires“. Journal Français d'Ophtalmologie 28 (März 2005): 245. http://dx.doi.org/10.1016/s0181-5512(05)74736-7.
Der volle Inhalt der QuelleDjairene, N., H. S. Cherif, F. Hamaidi-Chergui und S. Azrou. „Propriétés antiseptiques d’extrait éthanolique de Juglans regia (L.) et évaluation de son potentiel antibiofilm in vitro en implantologie orthopédique“. Phytothérapie, 2021. http://dx.doi.org/10.3166/phyto-2021-0298.
Der volle Inhalt der QuelleDissertationen zum Thema "Biofilms – Lutte contre"
Leroy, Céline. „Lutte contre les salissures marines : approche par procédés enzymatiques“. Toulouse, INSA, 2006. http://www.theses.fr/2006ISAT0002.
Der volle Inhalt der QuelleFouling on marine underwater surfaces causes critical and economic problems such as important material biodamages and industrial performances reduction. We chose to test antifouling potential of enzymatic commercial preparations like hydrolases (proteases, glycosidases and lipases) in order to inhibit the first fouling adhesion step: bacterial biofilm formation. An evaluation test of antifouling properties onto marine bacterial adhesion was designed using a mono-incubation of Pseudoalteromonas sp. D41 in microtiter plate and in sterile natural sea water. This test was adapted to screen agents for bacterial adhesion removal or inhibition activities and allowed to test enzymatic preparations toxicity on non adhered bacteria. Inhibition rates according to logarithm of enzymatic preparation concentration exhibits a sigmoid shape like dose-response curves. Among hydrolases, proteases like subtilisin are the most efficient enzymes. The efficiency of amylase, lipase and protease activity mixture was evaluated and showed a high synergistic inhibition on Pseudoalteromonas sp. D41 adhesion in microtiter plate. Studies on polymeric extracellular substances from Pseudoalteromonas sp. D41 in fermentation and in biofilm will be helpful in the understanding of the organic molecules nature involved in the adhesion inhibition
Simon, Marjolaine. „Lutte contre les biofilms de Pseudomonas aeruginosa dans le contexte de la mucoviscidose“. Thesis, Lorient, 2015. http://www.theses.fr/2015LORIS364.
Der volle Inhalt der QuellePseudomonas aeruginosa is an opportunistic pathogen leading to chronic infections in patients suffering of cystic fibrosis. Eradication of these infections is almost impossible in adults because of biofilm formation in patient's lungs. Current antibiotics treatments are not efficient enough against biofilms because this lifestyle first protects bacteria from antimicrobial agents via the biofilm extracellular matrix and secondly promotes the emergence of antibiotic resistance mechanisms. lt is therefore essential to find therapeutic alternatives . The marine bacterium Pseudoalteromonas sp. 3J6 secretes an anti-biofilm molecule active against the laboratory P. aeruginosa PA01 strain and the P. aeruginosa clinical strains MUC-N1, MUC-N2 and MUC P4. These strains were characterized at the levels of in vitro biofilm formation, and of their virulence. This part of the research work highlighted that these strains are different from one to another and that a single strain, such as the laboratory strain PA01, cannot be representative of the various patterns. Anti-biofilm studies have thus to be performed on several strains, such as the ones we selected. The therapeutic potential of the culture supernatant (SNa.Js} of Pseudoalteromonas sp. 3J6 and its extract Ea.Js was studied by evaluating their toxicity, inflammatory response, impact on virulence factors production, and therapeutic efficiency. SNa.Js and Ea.Js were not taxie against the tested models; did not induce inflammatory response in mice lungs, and did not enhance virulence factor production by clinical P. aerugonisa strains. Moreover, SNa.Js was as efficient as the ciprofloxacin antibiotic to treat an in vivo infection by P. aeruginosa MUC-N2 on mice. These results are encouraging as for a therapeutic potential of the anti-biofilm molecule to contribute at the treatment of P. aeruginosa infections of cystic fibrosis patients
Marion, Karine. „Evaluation des procédés actuels de lutte contre le biofilm dans les générateurs d'hémodialyse“. Paris 5, 2002. http://www.theses.fr/2002PA05P619.
Der volle Inhalt der QuelleBiofilms that develop on dialysis systems release endotoxins that are responsible for inflammatory diseases. The efficacy of the various "anti-biofilm" procedures proposed by the manufacturers have been assessed to help the users to make a choice. First of all, a biofilm analysis protocol was set-up, based on three main parameters : the coverage, the culturable cells and the endotoxin level. Tubing samples were removed from dialysis centers and analyzed. The highest contamination was observed on the samples coming from the connection between the water distribution loop and the dialysis machine; the cleanest samples had been disinfected by citric acid followed by autoclave. In vitro studies showed that no tested procedure was able to lead to a complete biofilm removal. Procedures using a hot disinfectant promote biofilm growth by "baking" the biofilm on the surface. The best treatments to be recommended are either the combination between bleach and a descaling agent, or the use of a Miroclav® machine
Farhat, Maha. „Étude de la survie des légionelles et de la dynamique des populations microbiennes des réseaux d'eau chaude : rôle des procédés de décontamination“. Poitiers, 2009. http://theses.edel.univ-poitiers.fr/theses/2009/Farhat-Maha/2009-Farhat-Maha-These.pdf.
Der volle Inhalt der QuelleLegionella is a Gram-negative bacterium identified in Legionnaires' outbreaks linked to contaminated hot water systems and cooling towers. In France, where this disease has been notifiable since 1987, over a thousand cases are reported per year among which 10 % are fatal. Legionella and other microorganisms are attached to the surface and associated together in an extracellular anionic polymer matrix called biofilm. Within this structure, Legionella is more protected from disinfection processes than those present in the aqueous phase. Therefore, our objectives were structured around three main parts: (1) to develop an analytical tool in order to evaluate the effectiveness of various anti-Legionella treatments in water and biofilm (2) to monitor the prokaryotic total flora evolution and Legionella in water and biofilm after heat shock (70 °C during 30 minutes) and chemical treatments (biocide combinated with biodispersant) and (3) to characterize Legionella diversity and its associated microbial flora dynamic (bacteria and eukarya) in biofilm after both treatments. A pilot scale 1 consisted of two hot water similar loops (control and test loops) was developed and contaminated by a natural biofilm of Legionella. Both treatments tested (heat and chemical) have had a transient effect. A return to the initial concentrations of culturable Legionella in water and in biofilm was observed one week after application of treatments. For the first time in such studies, the use of advanced molecular methods (sequencing, SSCP) has identified the diversity of Legionella (five of which four species are opportunistic pathogens) in biofilm sampled in hot water networks and the characterization of the microbial flora accompanying Legionella in this matrix (proteobacteria, amoebae, flagellates, fungi, Alveolata). This work brings together innovative elements in terms of tools to test the anti-Legionella, analytical methods and knowledge about the microbial consortium accompanying Legionella in the biofilm
Lopes, Julie. „Développement de nouvelles stratégies de lutte contre les biofilms de Providencia stuartii, un pathogène humain multi-résistant“. Thesis, Université Grenoble Alpes (ComUE), 2019. http://www.theses.fr/2019GREAV025/document.
Der volle Inhalt der QuelleBacterial biofilms are multicellular communities adherent to surfaces and surrounded by an extracellular matrix. They are crucial for maintaining most of our planet’s ecosystems, but also a threat to human health. Biofilm eradication is one of the greatest challenges of modern microbiology due to their high resistance to antibiotics. In this PhD, we focused on Providencia stuartii, a Gram-negative pathogen that forms biofilms in the human urinary tract and is responsible for about 10% of hospital-acquired urinary tract infections. P. stuartii exploits an additional means of socialization, forming floating communities of cells (FCCs), that later sediment and adhere onto surfaces yielding surface-attached biofilms (SABs). The two porins of P. stuartii, Omp-Pst1 and Omp-Pst2, are involved in FCC formation by self-association into intercellular dimers of trimers (DOTs). The main driving force behind DOT formation is electrostatic attraction, yet the DOT structure is locked-in by steric zipper interactions between facing extracellular loops.The two main objectives of this PhD were (i) to characterize the environmental impacts on the establishment and survival of the two type of socialized communities formed by P. stuartii, and (ii) to inhibit bacterial socialization by targeting porin DOTs. Our results reveal that peptides featuring residues involved in the steric zipper interaction of DOTs, and coupled with coumarin, are promising lead compound to diagnose P. stuartii infections. In addition, we tested combinations of antibiotics with some of these peptides and results suggest that is was a new therapeutic approach that can be envisaged to fight against P. stuartii infections. Future treatments should be evaluated on FCCs and SABs in conditions mimicking urinary tract, to be efficient and potentially eradicate P. stuartii
Hage, Mayssane. „Understanding the mechanisms of interactions at interfaces between bacteria and materials : development of anti-adhesion and anti-biofilm surfaces“. Electronic Thesis or Diss., Université de Lille (2018-2021), 2021. http://www.theses.fr/2021LILUR037.
Der volle Inhalt der QuelleThe operating environment in the food and medical fields allows bacteria to attach and grow on surfaces, resulting in the formation of pathogenic and resistant bacterial biofilms. These pathogenic structures are responsible for several foodborne illnesses and hospital-acquired infections. Therefore, to fight this public health scourge, one possible approach is the use of cold plasma technologies for the development of coatings on different materials. This work presents the different factors influencing bacterial adhesion to a substrate. In addition, strategies for the development of passive coatings to prevent biofilm formation by cold plasma surface treatments are described as well as the anti-adhesive properties of the developed surfaces. General aspects of coating, including physicochemical surface modifications and the use of cold plasma technologies, are also presented. In this context, a study was conducted to inhibit the adhesion of the pathogenic bacterium Salmonella enterica to the surface of stainless steel via cold plasma treatment. In order to limit the formation of Salmonella enterica biofilm, organosilicon coatings based on the monomer 1,1,3,3-tetramethyldisiloxane, mixed or not with oxygen, were elaborated by plasma polymerization with post-microwave nitrogen discharge. The effect of cold plasma parameters on coating properties, surface topography, and Salmonella enterica cell adhesion was studied. The results revealed that the surface topography significantly influenced the adhesion rate of bacteria. Indeed, rough surfaces did not inhibit Salmonella enterica adhesion as the number of cells adhering to these surfaces varied from 30 ± 4 to 65 ± 4 bacteria per microscopic field. On the other hand, an anti-adhesive behaviour towards Salmonella enterica was demonstrated for the smoother surfaces. Indeed, the number of attached cells was close to zero on these coatings. A complementary approach to this passive strategy of anti-adhesive surfaces is the development of active surfaces. Emerging technologies for effective active antimicrobial coatings are addressing the challenge of eliminating pathogenic biofilms formed on materials used in hospital and food processing environments. Stainless steel is a commonly used material in these fields, but unfortunately it has insufficient bio-functional properties, making it susceptible to bacterial adhesion and biofilm development. In this context, this thesis presents a review of coatings developed by employing biocides and antimicrobial peptides (AMPs) grafted onto stainless steel. In addition, a new active approach based on stainless steel coated with nisin, a common AMP accepted as a safe alternative to prevent the development of pathogenic biofilms, is developed. In this study, stainless steel surfaces were functionalized by nisin which was grafted to the surface either via its carboxyl group or via its amino group. Indeed, the surfaces coated with nisin grafted via its amino group showed a potent antibacterial activity while the surface grafted with nisin linked via its carboxyl group showed no antimicrobial effect. Analyses of the surface properties provided insight into the antibacterial effects, chemical and topographical characteristics of the treated surfaces, and the configuration and quantification of nisin
Paisse, Sandrine. „Etude de la réponse d’une communauté bactérienne de sédiments côtiers à une contamination pétrolière“. Pau, 2009. http://www.theses.fr/2009PAUU3015.
Der volle Inhalt der QuelleCoastal zones are highly productive environments chronically exposed to hydrocarbon contamination. Bacterial communities inhabiting these ecosystems play key role in biogeochemical processes and often form microbial mats in which microorganisminteractions are enhanced. In order to characterize the modifications occurring in the bacterial community during oil spill, the bacterial community response of Etang-de-Berre coastal sediment to oil contamination was studied based on in situ analysis of natural bacterial communities as well as on microcosm experiments. First the investigation of the in situ natural bacterial communities inhabiting sediments in a gradient of hydrocarbon concentrations revealed an important adaptation of the bacterial community to the oil contamination. We then explored the early response of the bacterial community to cope with the oil contamination. Analyses based on 16S rRNA genes and their transcripts revealed structural modifications occurring in the first hours and the first days following the contamination. The diversity and the expression of functional genes involved in hydrocarbon degradation such as alkane monooxygenase and naphthalene dioxygenase were investigated. These genes were immediately expressed in response to the oil addition while concomitantly high rates of hydrocarbon biodegradation were observed. Thus these results indicated important adaptative mechanisms during the first two days following the contamination. In order to further characterize this mechanism at the whole bacterial community level, we then performed the identification of genes repressed and/or overexpressed in response to oil addition
Mechmechani, Samah. „Hurdle technology using microencapsulated proteolytic enzymes and microencapsulated carvacrol to fight pathogenic bacterial biofilms“. Electronic Thesis or Diss., Université de Lille (2022-....), 2022. http://www.theses.fr/2022ULILR023.
Der volle Inhalt der QuelleThe ambient operating environments in the food and medical sectors allow bacteria to adhere and develop on the substrates, resulting in the growth of resistant pathogenic bacterial biofilms. These pathogenic structures are responsible for several foodborne diseases and health-care associated infections. Consequently, to combat this public health burden, several strategies have recently been proposed which include chemical and mechanical removal. This work presents the different factors that influence bacterial adhesion and biofilm formation on abiotic surfaces, as well as biofilm resistance to disinfectants. The different strategies for biofilm prevention and eradication are described. Microencapsulation using spray-drying method for the formulation of anti-biofilm active components as a tool to ensure their stability and improves their biological activities are also presented. In this context, a study was conducted using carvacrol, a natural antimicrobial agent, to control biofilms of Pseudomonas aeruginosa and Enterococcus faecalis. Indeed, these two bacteria are responsible for several infections worldwide due to their persistence on abiotic surfaces in hospitals and food processing industries. Furthermore, in order to enhance the antimicrobial activity of carvacrol and reduce its volatility and low solubility in water, feed emulsions were prepared with sodium caseinate and maltodextrins and then spray dried to obtain dry carvacrol microcapsules. The results showed that carvacrol had a strong antimicrobial activity against both bacterial biofilms. Furthermore, our findings revealed that microencapsulation by spray drying significantly increased the antimicrobial activity of carvacrol while reducing the amounts used. Indeed, microencapsulated carvacrol was able to reduce biofilm below the detection limit for Pseudomonas aeruginosa and 5.5 log CFU mL-1 for Enterococcus faecalis after 15 min of treatment. However, the complete removal of biofilms from abiotic surfaces in medical and food sectors has proven difficult with the single use of disinfection strategy due to the high protection of the biofilm cells by the extracellular polymeric matrix. This matrix provides an initial protective barrier for the biofilm cells, and makes biofilms highly resistant to antimicrobial agents. The effectiveness of hurdle technology in removing biofilms using different strategies is discussed in this work. One of the hurdle technology approaches is the use of matrix-degrading enzymes that can disperse bacteria embedded in biofilms for more efficient disinfection when combined with biocide agents. Indeed, two proteolytic enzymes, pepsin and trypsin, targeting matrix proteins, have been studied for their potential to degrade biofilms of Pseudomonas aeruginosa and Enterococcus faecalis and their synergistic effect when combined with carvacrol. The direct analysis using epifluorescence microscopy allowed visualization of the dispersive activity of proteases and the lethal activity of carvacrol against the two bacterial biofilms. In addition, the combined pepsin or trypsin treatment with carvacrol showed more significant reduction of both biofilms compared to carvacrol treatment alone. Moreover, this reduction was more substantial after sequential treatment of both enzymes followed by carvacrol. However, the enzyme activity is highly influenced by environmental factors and is only optimal under restricted conditions. Another disadvantage of using enzymes is self-degradation, leading to instability. Indeed, protease microcapsules containing pepsin or trypsin complexed with pectin and maltodextrin have been prepared
Jouault, Albane. „Altérocine : une protéine antibiofilm secrétée par la bactérie marine Pseudoalteromonas sp. 3J6“. Electronic Thesis or Diss., Lorient, 2019. http://www.theses.fr/2019LORIS588.
Der volle Inhalt der QuelleThe biofilm lifestyle gives bacteria a protection against antibacterial agents and leads to public health problems that require an alternative to current treatments. The marine bacterium Pseudoalteromonas sp. 3J6 and its exoproducts (SN3J6) display an antibiofilm activity against various bacteria from marine or terrestrial origin. A protein from SN3J6, named alterocin, was partially purified. Although the protein is found in several other Pseudoalteromonas strains, its function remains unknown. In this work, we studied the alterocin. We investigated the protein and gene characteristics at first. The gene alt, coding alterocin, is not part of an operon and several potential promoters were identified. According to our results, its expression seems subject to regulation as it is mainly expressed in stationary phase. It encodes a 139-residue protein with a putative leader peptide, which would allow the secretion of mature alterocin as a 119-residue protein. No sequence homology has been found between alterocin and other proteins of known function in data bases. Anti-alterocin antibodies were produced for an easily detection method. In a second time, we confirmed the antibiofilm activity of alterocin by heterologous production in another Pseudoalteromonas strain and comparing biofilms obtained in the presence of culture supernatants of either this strain or the parental strain. In this work, we showed that the alterocin is a new type of antibiofilm protein whose structure and mechanism of action remain to be elucidated to use it as antibiofilm agent
Yammine, Jina. „Nanoencapsulation of biosourced antimicrobials for a persistent disinfection of food contact surfaces“. Electronic Thesis or Diss., Université de Lille (2022-....), 2022. http://www.theses.fr/2022ULILR037.
Der volle Inhalt der QuelleThe persistence of biofilms remains a worldwide problematic encountered in the agro-food industry. As a result of the adaptive resistance coupled with the physical properties of biofilm matrix, the failure to eradicate totally biofilms using conventional disinfectants urges the need to find alternative effective strategies. The current methodology developed in this work is focused on the use of biosourced essential oil terpenes, namely carvacrol (CAR) and thymol (THY) that represent powerful antimicrobial tools facing biofilms. Nanoencapsulation of terpenes is an innovative and proactive approach that stabilizes terpenes and enhances their functionalities by protecting them within a carrier shell structure and by ensuring a sustained controlled release. The results of this work reveal a greater activity of nanoencapsulated CAR and THY against Salmonella Enteritidis and Listeria innocua biofilms developed on stainless steel (SS) surfaces as compared to the activity of free terpenes. The potent antimicrobial prospects of nanocapsules were highlighted by inducing major obvious structural damages to bacterial cells with subsequent increase in permeability, promoting the leakage of intracellular vital constituents to the outer medium. After confirming the promising antibiofilm activity of monolayer (ML) nanocapsules developed by spray-drying using maltodextrin as carrier material and sodium caseinate as emulsifier, another layer-by-layer (LBL) nanocapsule was developed by adding pectin as an additional interfacial layer. The increased shell structure thickness of the LBL capsules was observed microscopically and confirmed by the increase in size. The release kinetics of terpenes from the ML and LBL capsules fitted into a Korsmeyer-Peppas mathematical model dominated by a Fickian-diffusion mechanism. The diffusion of THY and CAR out of the ML and LBL capsules was ascribed to a biphasic release profile starting with an initial rapid burst release of terpenes, followed by a second phase of steady release from the ML capsules compared to a gradual sustained release over time from the LBL capsules. The antibiofilm activities of encapsulated THY and CAR were consistent with the release curves, highlighting a promising sustained disinfection of food contact surfaces. A successive exposure to ML and LBL capsules ensured a 99.99 % eradication of biofilms with a protection of SS surfaces from recontamination for several hours. The inhibition was induced by the ML nanocapsules that ensured an initial disinfection of surfaces with a reduction of bacterial biofilms within the first exposure minutes, combined with the LBL capsules that kept releasing terpenes in a controlled manner over several hours favoring a sustained prolonged disinfection of food contact surfaces and a protection from bacterial recontamination. The prominent persistent disinfection activity using a successive treatment of ML and LBL nanocapsules was also validated on biofilms developed under different hydrodynamic conditions in a lab-scale pipeline system set-up to mimic some of the real flow conditions encountered in agro-food industries
Bücher zum Thema "Biofilms – Lutte contre"
Bott, T. R. Industrial biofouling. Amsterdam: Elsevier, 2011.
Den vollen Inhalt der Quelle findenDevinny, Joseph S. Biofiltration for air pollution control. Boca Raton, Fla: Lewis Publishers, 1999.
Den vollen Inhalt der Quelle findenDevinny, Joseph S., Marc A. Deshusses und Todd S. Webster. Biofiltration for Air Pollution Control. Taylor & Francis Group, 2020.
Den vollen Inhalt der Quelle findenBuchteile zum Thema "Biofilms – Lutte contre"
Debiemme-Chouvy, Catherine. „17 Les surfaces antimicrobiennes : un atout dans la lutte contre le développement des biofilms“. In Interactions Matériaux-Microorganismes, 371–94. EDP Sciences, 2020. http://dx.doi.org/10.1051/978-2-7598-2112-9-019.
Der volle Inhalt der QuelleDebiemme-Chouvy, Catherine. „17 Les surfaces antimicrobiennes : un atout dans la lutte contre le développement des biofilms“. In Interactions Matériaux-Microorganismes, 371–94. EDP Sciences, 2020. http://dx.doi.org/10.1051/978-2-7598-2112-9.c019.
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