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TARAKANOV, R. I., A. N. IGNATOV und F. S. DZHALILOV. „ISOLATION OF SPECIFIC BACTERIOPHAGES - PSEUDOMONAS SAVASTANOI PV. GLYCINEA - AND THEIR USE IN SOYBEAN BACTERIAL BLIGHT CONTROL“. Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, Nr. 4 (2020): 43–53. http://dx.doi.org/10.26897/0021-342x-2020-4-43-53.
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Bacterial blight is one of most harmful diseases of legumes, reducing the profitability of soybean production in Russian Federation. Among a number of Pseudomonas isolates obtained from diseased seeds and plants of soybean, 4 strains were selected and confirmed as Pseudomonas savastanoipv. glycinea (Psg). Properties of the isolated bacteria were similar to type strain of Psg CFBP 2214 in plant virulence, LOPAT tests, and PCR analysis for coronafacate ligase gene, and partly – in the phage reaction profile. Four isolates of bacteriophages specific to Psg were obtained from soil samples taken from fields with soybean crops. Virulence testing for the bacteriophages showed that bacteriophage ϕG17 infected 4 of 5 tested Psg strains, and it was chosen for further experiments with bacterial blight control. The bacteriophague effect control conducted on soybent plants inoculated by Psg experiments confirmed that 2 treatments of plants by the bacteriophage significantly reduced the disease development. Biological effect of the bacteriophage application was 74.75%, which is very close to the pesticide Strekar in a concentration of 0.5%.
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Samir, Safia. „Bacteriophages as Therapeutic Agents: Alternatives to Antibiotics“. Recent Patents on Biotechnology 15, Nr. 1 (07.05.2021): 25–33. http://dx.doi.org/10.2174/1872208315666210121094311.
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: Bacteriophages are bacterio-specific viruses that constitute the main portion of the environment. Bacteriophages inject their genome into the targeted bacterial cells and some of them can disrupt the metabolism of bacteria and cause bacterial cell disintegration. The application of bacteriophages to kill bacteria is known as bacteriophage therapy. Since bacteriophages target bacteria and are strain-specific, every bacteriophage/bacterial host pair is unique. They are believed to cause no harm to humans. An additional advantage of the strain-specific nature of bacteriophages is that they do not disrupt the beneficial natural flora in the body. Bacteriophage therapy in the West is not a recognized medicine at this time, and no products are registered. Some clinicians are turning to bacteriophage therapy for the treatment of antibiotic-resistant infections. Lack of adverse effects makes bacteriophage therapy ideal for use. Funding research, media attention, and the increased publication of articles helped in a widespread understanding of its therapeutic potential. The first prerequisite for the use of bacteriophage therapy is simply the availability of bacteriophages for treatment, which is often complicated at this stage of bacteriophage production. This includes providing access to all biologically active bacteriophages against the bacterial isolate of the patient and meeting regulatory criteria of purity, traceability, and characterization. A monophage preparation, which is a single bacteriophage, or a phage cocktail, which consists of a number of combined bacteriophages against one or more bacterial species may be used. Accordingly, the antibiotic resistance crisis brought back bacteriophage therapy as a potential complementary or alternative treatment. Bacteriophages are promising cheap antibacterials.
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Li, Jinyu, und John J. Dennehy. „Differential Bacteriophage Mortality on Exposure to Copper“. Applied and Environmental Microbiology 77, Nr. 19 (12.08.2011): 6878–83. http://dx.doi.org/10.1128/aem.05661-11.
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ABSTRACTMany studies report that copper can be used to control microbial growth, including that of viruses. We determined the rates of copper-mediated inactivation for a wide range of bacteriophages. We used two methods to test the effect of copper on bacteriophage survival. One method involved placing small volumes of bacteriophage lysate on copper and stainless steel coupons. Following exposure, metal coupons were rinsed with lysogeny broth, and the resulting fluid was serially diluted and plated on agar with the corresponding bacterial host. The second method involved adding copper sulfate (CuSO4) to bacteriophage lysates to a final concentration of 5 mM. Aliquots were removed from the mixture, serially diluted, and plated with the appropriate bacterial host. Significant mortality was observed among the double-stranded RNA (dsRNA) bacteriophages Φ6 and Φ8, the single-stranded RNA (ssRNA) bacteriophage PP7, the ssDNA bacteriophage ΦX174, and the dsDNA bacteriophage PM2. However, the dsDNA bacteriophages PRD1, T4, and λ were relatively unaffected by copper. Interestingly, lipid-containing bacteriophages were most susceptible to copper toxicity. In addition, in the first experimental method, the pattern of bacteriophage Φ6 survival over time showed a plateau in mortality after lysates dried out. This finding suggests that copper's effect on bacteriophage is mediated by the presence of water.
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Gorshenin, Aleksandr Vladimirovich. „Participation of microbiologists Z.V. Ermolyeva and L.M. Yakobson in a scientific discussion about the fate of the production of Soviet cholera bacteriophages in 1967“. Samara Journal of Science 10, Nr. 4 (01.12.2021): 201–7. http://dx.doi.org/10.17816/snv2021104211.
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Since the late 1920s in the USSR, along with many countries, there has been an interest in studying the phenomenon of bacteriophagy and its use in medicine. Bacteriophages are called bacterial viruses. Significant work on the study of bacteriophages and their use for medical purposes was carried out by Soviet microbiologists Zinaida Vissarionovna Ermolyeva and Lidiya Mikhailovna Yakobson. They paid especially great attention to the study of cholera bacteriophage, which during the Great Patriotic War helped prevent the cholera epidemic in frontline Stalingrad. In the 1940s due to the advent of the era of antibiotics with a wider range of applications, research interest in bacteriophages was waning. Nevertheless, in the prevention and treatment of a number of infectious diseases of bacterial origin, phage continued to be used in the following decades. In 1967, one of the countrys largest microbiologists sent an appeal to the Chief Sanitary Doctor of the USSR with a proposal to stop or reduce the production of cholera bacteriophage in the country. In this regard, a scientific discussion in which Z.V. Ermolyeva and L.M. Yakobson played an important role unfolded. Using the materials of the State Archive of the Russian Federation and the Russian State Archive of Economics, involving published scientific works on microbiology and medicine, an attempt is made to analyze this scientific discussion and establish its significance in the fate of the production of cholera bacteriophage in the USSR.
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Xu, Anqi. „Draft Of Bacteriophage Therapy Review“. Highlights in Science, Engineering and Technology 74 (29.12.2023): 1105–16. http://dx.doi.org/10.54097/hfwfxj54.
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Phage therapy, which uses bacterial viruses (bacteriophages) to treat bacterial infections. In recent years, the issue of antibiotic resistance in various bacteria has become prominent, and the effectiveness of antibiotic drugs has generally declined, which has re raised people's scrutiny of bacteriophage therapy. Traditionally, bacteriophage therapy relies on the use of natural bacteriophages to infect and dissolve bacteria at the site of infection. Current research on the use of bacteriophages and their lytic proteins to combat multidrug-resistant bacterial infections suggests that bacteriophage therapy may serve as a substitute or supplement to antibiotic therapy.
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Moon, Choi, Jeong, Sohn, Han und Oh. „Research Progress of M13 Bacteriophage-Based Biosensors“. Nanomaterials 9, Nr. 10 (11.10.2019): 1448. http://dx.doi.org/10.3390/nano9101448.
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Recently, new virus-based sensor systems that operate on M13 bacteriophage infrastructure have attracted considerable attention. These systems can detect a range of chemicals with excellent sensitivity and selectivity. Filaments consistent with M13 bacteriophages can be ordered by highly established forms of self-assembly. This allows M13 bacteriophages to build a homogeneous distribution and infiltrate the network structure of nanostructures under mild conditions. Phage display, involving the genetic engineering of M13 bacteriophages, is another strong feature of the M13 bacteriophage as a functional building block. The numerous genetic modification possibilities of M13 bacteriophages are clearly the key features, and far more applications are envisaged. This paper reviews the recent progress in the application of the M13 bacteriophage self-assembly structures through to sensor systems and discusses future M13 bacteriophage technology.
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Khan, Muhammad Fayaz, Aamer Ali Khattak, Afshan Saleem, Muhammad Rizwan, Muhammad Asif und Iqbal Ahmad Alvi. „Efficient Reduction of Pseudomonas aeruginosa Biofilms Using the Myoviridae Lytic Bacteriophage vBPaeM MLG“. Lahore Garrison University Journal of Life Sciences 7, Nr. 03 (25.09.2023): 262–79. http://dx.doi.org/10.54692/lgujls.2023.0703275.
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Pseudomonas aeruginosa biofilm infections pose significant challenges in clinical settings due to their increased resistance to conventional antibiotics. Bacteriophages, viruses that infect and kill bacteria, have emerged as promising agents for combating biofilm-related infections. This study aimed to isolate and characterize a potent bacteriophage with antibiofilm activity against P. aeruginosa. Hospital sewage was utilized to isolate a bacteriophage targeting P. aeruginosa. Quantification of phages was conducted through spot tests and doublelayer agar methods. The stability of the isolated phage was assessed under varying pH and temperature conditions. Furthermore, the bacteriophage's ability to reduce bacterial growth and exhibit antibiofilm activity was evaluated at different Multiplicity of Infection (MOI) levels. The isolated bacteriophage, named MLG, was identified as a member of the Myoviridae family within the Caudovirales order. MLG effectively reduced bacterial growth over a 14-hour period. It displayed tolerance to a pH range of 5 to 9 and temperatures spanning 25 to 60°C. Moreover, MLG demonstrated efficient inhibition of biofilm formation across various MOI levels. Given its demonstrated in vitro capacity for bacterial growth reduction and antibiofilm activity, MLG holds potential for combatting P. aeruginosa biofilm infections. This study suggests a promising avenue for the development of alternative antibiofilm strategies using bacteriophages.
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Jamaledin, Rezvan, Rossella Sartorius, Concetta Di Natale, Raffaele Vecchione, Piergiuseppe De Berardinis und Paolo Antonio Netti. „Recombinant Filamentous Bacteriophages Encapsulated in Biodegradable Polymeric Microparticles for Stimulation of Innate and Adaptive Immune Responses“. Microorganisms 8, Nr. 5 (29.04.2020): 650. http://dx.doi.org/10.3390/microorganisms8050650.
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Escherichia coli filamentous bacteriophages (M13, f1, or fd) have attracted tremendous attention from vaccinologists as a promising immunogenic carrier and vaccine delivery vehicle with vast possible applications in the development of vaccines. The use of fd bacteriophage as an antigen delivery system is based on a modification of bacteriophage display technology. In particular, it is designed to express multiple copies of exogenous peptides (or polypeptides) covalently linked to viral capsid proteins. This study for the first time proposes the use of microparticles (MPs) made of poly (lactic-co-glycolic acid) (PLGA) to encapsulate fd bacteriophage. Bacteriophage–PLGA MPs were synthesized by a water in oil in water (w1/o/w2) emulsion technique, and their morphological properties were analyzed by confocal and scanning electron microscopy (SEM). Moreover, phage integrity, encapsulation efficiency, and release were investigated. Using recombinant bacteriophages expressing the ovalbumin (OVA) antigenic determinant, we demonstrated the immunogenicity of the encapsulated bacteriophage after being released by MPs. Our results reveal that encapsulated bacteriophages are stable and retain their immunogenic properties. Bacteriophage-encapsulated PLGA microparticles may thus represent an important tool for the development of different bacteriophage-based vaccine platforms.
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SAKMANOĞLU, Aslı, und Hasan Hüseyin HADİMLİ. „Typing of ΦSP–3 lytic Salmonella bacteriophages obtained from various fecal sources“. TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES 44, Nr. 5 (27.10.2020): 1047–54. http://dx.doi.org/10.3906/vet-2005-105.
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Although several reports are available on both ΦSP–1 and ΦSP–3 lytic Salmonella bacteriophages obtained from poultry, further research is required to study the effectiveness of ΦSP–3 type on serovars isolated from other sources. In the present study, we aimed to isolate bacteriophages from 8 serovars previously obtained from 869 fecal samples (calf, dairy cow, buffalo, and camel), genotype the bacteriophages, and detect the cross-lytic activities of the bacteriophages on Salmonella enterica subsp. enterica serovar Kentucky, S.Anatum, and S.Muenchen. A total of 16 bacteriophages were detected as ΦSP–3 type via PCR. The Hunter-Gaston Discriminatory Index of SDS-PAGE was calculated to be 0.825. Determination of multiplicity of infection (MOI) values were different for each bacteriophage according to the cross-lytic activity assessment. The MOI of the most effective S. Kentucky bacteriophage was 79.11 μg/mL for 2.5×104 cells, whereas that of the most ineffective S.Muenchen bacteriophage was 1.142 μg/μL for 2.5×104 cells. In conclusion, it was assumed that owing to the high and cross-lytic activity of the S. Kentucky bacteriophage, it has a larger host range, which differs in the lytic activities of each bacteriophage, despite being the same serovar, and that calf feces is the most important source for obtaining Salmonella bacteriophages.
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Kargina, T. M., E. I. Sakanyan, D. S. Davydov und R. L. Parfenyuk. „Elaboration of Pharmacopoeial Quality Standards for Bacteriophage Products“. BIOpreparations. Prevention, Diagnosis, Treatment 19, Nr. 4 (11.12.2019): 233–41. http://dx.doi.org/10.30895/2221-996x-2019-19-4-233-241.
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Bacteriophages are novel safe and efficacious medicinal products that are used for treatment of intestinal infections and purulent inflammations. The fact that virulent phages can be adapted to fight antibiotic-resistant bacterial strains makes this group of medicines a promising means of treatment of infections associated with medical interventions. The elaboration of quality standards for bacteriophage products will enable alignment of the quality requirements and test methods. There are no monographs on bacteriophage products in pharmacopoeias of other countries, therefore, the development of general chapters on groups of test methods used in bacteriophage quality control and monographs on bacteriophages for the State Pharmacopoeia of the Russian Federation (Ph. Rus.) was a very relevant and timely initiative. The aim of the study was to elaborate pharmacopoeial quality standards for bacteriophages approved in the Russian Federation for therapeutic and prophylactic indications. The authors of the study analysed product specification files and master production records for bacteriophages produced in the Russian Federation. They determined common GMP-compliant production steps, the selection criteria for bacteriophage strains and bacteria production strains, and cultivation and storage conditions. The authors standardised bacteriophage quality parameters and brought the test methods in line with the test procedures described in the Ph. Rus., 14th ed. The study summarised test methods used for identification of bacteriophages and determination of their specific activity. The main results of the study were included into the general monograph «Bacteriophages» and individual monographs on bacteriophage products that were included into the current edition of the Ph. Rus. Further studies and elaboration of new quality standards for mono- and multicomponent bacteriophage products, and the use of such products in clinical practice will improve prophylaxis and treatment of various infectious diseases.
Cramer, Todd James Lucas. „Genetic mosaicism between the bacteriophage [phi]80 and bacteriophage [lambda]“. Bowling Green, Ohio : Bowling Green State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1223514067.
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Cramer, Todd James. „Genetic Mosaicism Between The Bacteriophage φ80 And Bacteriophage λ“. Bowling Green State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1223514067.
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Phage therapy is the use of bacteriophages to treat bacterial infections. Once a prominent method of antibacterial therapy, phage therapy became almost forgotten following the discovery of antibiotics in 1928. The rising instance of antibiotic resistant bacteria in the last number of years has resulted in resurgence in interest in phage therapy. Research into delivery methods for bacteriophages has been very limited. Up to very recently, phages had only been delivered parentrally using crude and purified phage stocks and orally for gastrointestinal infections. In order for phage therapy to become suitable for use in mainstream medicine, suitable dosage forms and phage delivery platforms must be developed. This research project endeavoured to successfully formulate a model bacteriophage, T4 phage, into useful polymeric delivery systems, beginning with an alginate based microparticulate system. Bacteriophages were successfully stabilized within a Ca-alginate-trehalose system and m icroparticles were fully characterised. The release profi le of these m icroparticles suggest that they could be used to successfully treat gastrointestinal infections. The present study also devised a phage stable soluble microneedle system. It was found that increasing the trehalose concentration within the microneedles, improved rnicroneedle strength, as did the addition of a Poly (methyl vinayl ether-eo-maleic acid) backing layer. A poly(carbonate) hollow rnicroneedle system was used to successfully deliver phages both in vitro and in vivo using a rat model. This study was the first time that bacteriophages have been delivered transdermally in vivo. Aside from the development of bacteriophage delivery systems, novel work in the application of Phage-Antibiotic synergy (PAS) to E.co/i biofilrns was completed with extremely successful results. It was found that administering T4 bacteriophage in combination with the antibiotic Cefotaxime improved biofilrn eradication, compared to treatment with bacteriophage or antibiotic alone. This is the first time that PAS has been applied to a biofilm model.
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Thanki, Anisha M. „Development of a phage-based diagnostic test for the identification of Clostridium difficile“. Thesis, Loughborough University, 2016. https://dspace.lboro.ac.uk/2134/20340.
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Clostridium difficile is the most common bacterial cause of infectious diarrhoea in healthcare environments and in 2014 was responsible for 13,785 infections in the UK. C. difficile infection (CDI) is spread via the faecal-oral route and by contact with contaminated surfaces. However, despite the healthcare concerns no tests are available to validate if sufficient cleaning has been conducted. In addition, Polymerase Chain Reaction (PCR) and Enzyme Immunoassays (EIAs)-based tests used to diagnose CDI lack sensitivity and specificity and hence false negative results are commonly obtained. To overcome these concerns the aim of the PhD research has been to develop the first diagnostic test that exploits the specific interactions of C. difficile bacteriophages (phages), viruses that specifically infect and kill C. difficile. In order to develop a C. difficile phage-based test, first suitable phages that can be used for the test were identified and this was conducted by screening 35 different C. difficile phages against 160 clinically relevant C. difficile isolates. Five phages were found to infect the most number of isolates and were investigated further to identify whether a phage-based diagnostic could be developed based on phages binding (adsorption) to different C. difficile subgroups. However, for all five phages, adsorption rates were not consistently high for C. difficile subgroups in comparison to other common bacteria found in similar locations to C. difficile. Therefore, to increase specificity of the phage-based diagnostic test a new approach was taken by tagging two phages with luminescence luxAB genes (reporter phages), which would be expressed once C. difficile cells were infected with the phages. To design the C. difficile reporter phages, non-essential phage genes were replaced with the luxAB genes, but this study revealed mutagenesis of C. difficile was troublesome and extensive optimisation was required. In addition, once the reporter phages had successfully been constructed the luxAB genes were unstable within the phage genome and were lost during phage replication. Despite extensive optimisation and due to time constrains the luxAB genes were not stabilised within the phages but future work will focus on stabilising the genes.
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Terry, Tamsin Deborah. „Peptide display on filamentous bacteriophage“. Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627599.
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Nemavhulani, Shonisani. „Bacteriophage diversity in haloalkaline environments“. University of the Western Cape, 2013. http://hdl.handle.net/11394/4313.
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>Magister Scientiae - MSc There are limited reports on virus population in haloalkaline environments;
therefore the aim of this study was to investigate the genetic diversity and
biology of bacteriophage communities in these environments. Bacteria were
isolated to be used as phage hosts. One bacterium from Lake Magadi and four
bacteria from Lake Shala were successfully isolated from sediment samples. A
further two Lake Shala bacterial hosts from the IMBM culture collection were
also used to isolate bacteriophages. Bacterial isolates were identified to be
most closely related to Bacillius halodurans, Halomonas axialensis,
Virgibacillus salarius, Bacillus licheniformis, Halomonas venusta, Bacillus
pseudofirmus and Paracoccus aminovorans. Bacteriophages were screened
using all bacteria against sediment samples from both Lake Shala and Lake
Magadi. One phage was identified from Lake Magadi sediments (MGBH1) and
two phages from Lake Shala sediments (SHBH1 and SHPA). TEM analysis
showed that these phages belong to three different dsDNA phage families;
Siphoviridae (MGBH1), Myoviridae (SHBH1) and Podoviridae (SHPA). All
phages showed different genome sizes on agarose gel. Due to the small
genome size, phage SHPA was chosen for further investigation. Partial,
genome sequence analysis showed homology to both bacterial and phage
proteins. A further investigation of phage diversity in this environment is
essential using metagenomic approaches to understand these unique
communities.
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Abedon, Stephen Tobias. „The ecology of bacteriophage T4“. Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/185040.
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In this dissertation I explore the ecology of bacteriophage T4, a virus of Escherichia coli. In particular, I argue that the life history of bacteriophage T4 can be divided into the growth and survival of T4 phages in three distinct environments. I argue that these environments are distinguished by at least two T4 phage sensory systems. These include (i) the sensing of secondary adsorption by infecting phages and (ii) the determination of the concentration of monovalent cations and free tryptophan in solution about free T4 phage particles. The first environment consists of high concentrations of uninfected, logarithmic phase E. coli cells. These concentrations are approximately 10⁶ E. coli cells/ml and greater. This environment occurs in the prefecal colonic lumen of animals. Here T4 phages exhibit unimpeded logarithmic growth. The second environment contains high concentrations of infected E. coli cells, low concentrations of uninfected E. coli cells, and high concentrations of free T4 phage particles. This second environment also occurs in the prefecal colonic lumen of animals and represents the maturation of environments supporting logarithmic T4 phage population growth. Such phage phenotypes as secondary exclusion and lysis inhibition characterize T4 phage growth in this environment. The third environment consists of extra-colonic waters. Here T4 phages avoid infecting E. coli cells and exhibit strategies that maximize their stability. These strategies in extra-colonic waters increase the potential of T4 phages to disseminate successfully from colon to colon. I employ this enhanced understanding of T4 phage ecology, outlined above, in an exploration of the ecology of the repair of DNA damage by T4 phages.
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Forghani, Farnaz. „Protein engineering of bacteriophage Mu transposase“. Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=60444.
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Bacteriophage Mu is an ideal system to study DNA transposition. The 70-KDa protein product of the phage early gene A, termed transposase, is absolutely required for transposition. Transposase binds specifically at sites located at both ends of the phage genome, termed attL and attR, and at an enhancer-like element at the left end of the genome, called IAS (internal activation sequence). It then nicks at these ends, and nicks a random target DNA sequence in a 5 base pair staggered fashion with 5$ sp prime$ extensions and promotes strand transfer between the Mu ends and the target DNA. The transposase protein can be roughly divided into three domains. The other activities of the protein have not been mapped even at the domain level. To further define the different functional domains of this complex enzyme, a series of insertion mutants at 8 different sites along the transposase protein were constructed using TAB linker mutagenesis. In this study, 1 and 2 TAB linkers were inserted into 8 HpaII sites in the Mu A gene, generating a set of 2 and 4 amino acid insertion mutants. Examination of these mutants for specific DNA-binding activity of transposase to the ends of the phage genome in vitro revealed temperature sensitive proteins. Transpositional activity of the mutant proteins revealed that the mutant proteins, which are temperature sensitive in specific DNA-binding activity, are deficient in transpositional activity.
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Long, Graham Stanley. „Molecular cloning of bacteriophage K1E endosialidase“. Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339539.
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Azeredo, Joana, und Sanna Sillankorva, Hrsg. Bacteriophage Therapy. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7395-8.
Klaenhammer, T. R., und G. F. Fitzgerald. „Bacteriophages and bacteriophage resistance“. In Genetics and Biotechnology of Lactic Acid Bacteria, 106–68. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1340-3_3.
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Berry, Colin, Jason M. Meyer, Marjorie A. Hoy, John B. Heppner, William Tinzaara, Clifford S. Gold, Clifford S. Gold et al. „Bacteriophage“. In Encyclopedia of Entomology, 350. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_215.
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Gooch, Jan W. „Bacteriophage“. In Encyclopedic Dictionary of Polymers, 877. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13227.
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Dennehy, John J., und Stephen T. Abedon. „Bacteriophage Ecology“. In Bacteriophages, 1–42. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-319-40598-8_8-1.
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Dennehy, John J., und Stephen T. Abedon. „Bacteriophage Ecology“. In Bacteriophages, 253–94. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-319-41986-2_8.
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Berry, Colin, Jason M. Meyer, Marjorie A. Hoy, John B. Heppner, William Tinzaara, Clifford S. Gold, Clifford S. Gold et al. „Bacteriophage Wo“. In Encyclopedia of Entomology, 350–52. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_216.
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Frank, J. Howard, J. Howard Frank, Michael C. Thomas, Allan A. Yousten, F. William Howard, Robin M. Giblin-davis, John B. Heppner et al. „Phage (Bacteriophage)“. In Encyclopedia of Entomology, 2823. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_2878.
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Kiino, Diane R., und Lucia B. Rothman-Denes. „Bacteriophage N4“. In The Bacteriophages, 457–74. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5490-1_7.
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Konferenzberichte zum Thema "Bacteriophage":
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Shydlovska, Olga, und Yuliia Khmelnytska. „L. lactis Bacteriophages and Methods of Their Elimination from Dairy Products“. In The 9th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2022. http://dx.doi.org/10.24264/icams-2022.ii.23.
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Dairy products are important in human diet and nutrition. That is why dairy production is critical not only economically, but also socially and medically. In recent decades, dairy production has had problems with disturbances in fermentation processes caused by bacteriophage contamination. It is important to note that every year there are new reports about newly discovered bacteriophages that disrupt fermentation processes in the production of kefir, yogurt, and various types of cheese. Lactococcus lactis strains are of particular importance in dairy technology, as they are used for the production of various yogurts and cheeses. The study of the spectrum of bacteriophages infecting this strain can help to monitor the evolutionary changes of viruses and the horizontal transfer of genes. In this paper, an analysis of phages infecting L. lactis was carried out. Most bacteriophages belong to the Siphoviridae and Podoviridae families. Moreover, the authors analyzed approaches that can be used to reduce bacteriophage contamination in the production of dairy products. It has been shown that the use of disinfectants, such as ethanol on sodium hypochlorite, can reduce the titer of bacteriophages and protect products from the development of viral infection. It is also possible to use membrane filtration with UV irradiation. Moreover, all these approaches can be combined to achieve the most effective result.
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Orynbayev, A. T., K. A. Miroshnikov, A. N. Ignatov und F. S. Dzhalilov. „Evaluation of effectiveness of bacteriophage agent for cabbage black rot control“. In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-113.
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Twenty-one isolates of bacteriophages specific to eleven target strains of Xanthomonas campestris pv. campestris were isolated from soil samples collected under black rot-infected cabbage plants. After the analysis of phagotyping for seventy-three phytopathogen strains against newly isolated isolates and four collection strains of bacteriophages, it was proposed to construct a phage cocktail including 6 isolates In vitro screening of protective from ultraviolet radiation substances under the UV-B range showed that skim milk (0.75%), Riboflavin (0.5%) and ascorbic acid (0.1%) showed the highest effect for bacteriophages. Under the conditions of a film greenhouse, the best protective effect from solar UV radiation on the 8th day after spraying cabbage was shown by the option with the addition of skimmed milk (0.75%) and Riboflavin (0.5%). According to our data, these substances can provide a long-term photoprotective effect of the bacteriophage preparation..
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Carlson, Hans, Denish Piya, Madeline Moore, Adam Deutschbauer, Adam Arkin und Vivek Mutalik. „Geochemical constraints on bacteriophage infectivity“. In Goldschmidt2021. France: European Association of Geochemistry, 2021. http://dx.doi.org/10.7185/gold2021.7854.
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Tarakanov., R. I., A. N. Ignatov und F. S. Dzhalilov. „Development of bacteriophage agent for soyean bacterial blight control“. In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-136.
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There is a description for the method for development of bacteriophage agent to control bacterial blight of soybean caused by Pseudomonas savastanoi pv. glycinea. Biological effectiveness of bacteriophage application was about 75% that was approximately the same as for test chemical bactericides.
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Scott, J., und Y. Chenelot. „Bacteriophage control of pathogens in foods“. In International Smoked Seafood Conference. Alaska Sea Grant, University of Alaska Fairbanks, 2008. http://dx.doi.org/10.4027/isscp.2008.05.
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Stanley, G. L., M. Modak, B. K. Chan, I. Ott, Y. Sun, Z. Harris, K. Kortright, P. E. Turner und J. L. Koff. „Bacteriophage Decrease Cystic Fibrosis Lung Inflammation“. In American Thoracic Society 2021 International Conference, May 14-19, 2021 - San Diego, CA. American Thoracic Society, 2021. http://dx.doi.org/10.1164/ajrccm-conference.2021.203.1_meetingabstracts.a1215.
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Shabani, Arghavan, Mohammed Zourob, Beatrice Allain, Marcus Lawrence und Rosemonde Mandeville. „Electrochemical Detection of Bacteria Using Bacteriophage“. In 2007 International Symposium on Signals, Systems, and Electronics, URSI ISSSE 2007. IEEE, 2007. http://dx.doi.org/10.1109/issse.2007.4294410.
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Ismail Umlai, Umm-kulthum, und Annette Vincent. „Bacteriophage Diversity In The Ecology Of Qatar“. In Qatar Foundation Annual Research Conference Proceedings. Hamad bin Khalifa University Press (HBKU Press), 2014. http://dx.doi.org/10.5339/qfarc.2014.eesp0770.
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Stanley, G. L., B. Chan, I. Ott, E. Mayo, Z. M. Harris, Y. Sun, B. Hu, G. Rajagopalan, P. Turner und J. L. Koff. „Bacteriophage Therapy Decreases Pseudomonas Aeruginosa Lung Inflammation“. In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a2977.
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Kuhn, Jonathan, Rachel Broza und Ekaterina Verkin. „Making temporal maps using bacterial luciferase: Bacteriophage“. In Biomedical Optics 2004, herausgegeben von Alexander P. Savitsky, Lubov Y. Brovko, Darryl J. Bornhop, Ramesh Raghavachari und Samuel I. Achilefu. SPIE, 2004. http://dx.doi.org/10.1117/12.530706.
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Berichte der Organisationen zum Thema "Bacteriophage":
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Nasser, Abidelfatah, Charles Gerba, Badri Fattal, Tian-Chyi Yeh und Uri Mingelgrin. Biocolloids Transport to Groundwater. United States Department of Agriculture, Dezember 1997. http://dx.doi.org/10.32747/1997.7695834.bard.
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The first phase of the study was designed to determine the adsorption rate of viruses and microspheres to sandy and loamy soils and determine the adsorption efficiency of various viruses to soil. The adsorption of viruses to sandy and loamy soils has been found virus type dependent. The poorest adsorption was observed for MS2 bacteriophage while the greatest adsorption was observed for PRD-1. Adsorption sites on the soil material were not found as limiting factors for adsorption of viruses on soil material. The effect of water quality on adsorption has been found as virus type dependent. The adsorption process of microspheres to soil material has been found to be similar to that of viruses and occurs within a very short period of time. Carboxylated (negatively charged) microspheres seems to adsorb more efficiently than plain microspheres to soil material. At low temperatures (10oC), and under saturated conditions no virus die-off was observed, therefore under these conditions virus can survive for long period of time. At 23oC, and saturated conditions, the greatest die-off was observed for MS2 bacteriophage, whereas, negligible die-off was for PRD-1 bacteriophage and hepatitis A virus. Considering the survival results MS2 bacteriophages is not suitable as indicator for pathogenic viruses persistence in soil material. Furthermore, temperature, is more important than any other factor for the inactivation of viruses.
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Schnider, Shirley. The biological properties of Pseudomonas aeruginosa bacteriophage 7V. Portland State University Library, Januar 2000. http://dx.doi.org/10.15760/etd.771.
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Wick, Charles H., und Patrick E. McCubbin. Removing Complex Growth Media from MS2 Bacteriophage Cultures. Fort Belvoir, VA: Defense Technical Information Center, August 1999. http://dx.doi.org/10.21236/ada368537.
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Wick, Charles H., und Patrick E. McCubbin. Filtration Characteristics of MS2 Bacteriophage Using Various Molecular Weight Filters. Fort Belvoir, VA: Defense Technical Information Center, August 1999. http://dx.doi.org/10.21236/ada368535.
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Benson, Deanne. A study of RNA bacteriophage 7s infection of Pseudomonas aeruginosa. Portland State University Library, Januar 2000. http://dx.doi.org/10.15760/etd.2139.
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McFarland, Lynne. Purification and properties of lysozyme from Pseudomonas aeruginosa bacteriophage 7v. Portland State University Library, Januar 2000. http://dx.doi.org/10.15760/etd.2982.
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Jabbour, Rabih E., Deborah Kuzmanovic, Patrick E. McCubbin, Ilya Elashvili und Charles H. Wick. Mass Spectrometry and Integrated Virus Detection System Characterization of MS2 Bacteriophage. Fort Belvoir, VA: Defense Technical Information Center, August 2005. http://dx.doi.org/10.21236/ada439894.
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Obringer, John W. Bacteriophage T4D Gene 42 Mutants Exhibit a Defective Genetic Exclusion Phenotype. Fort Belvoir, VA: Defense Technical Information Center, Februar 1991. http://dx.doi.org/10.21236/ada235836.
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Farkas, Michelle E. Chemically Modified Bacteriophage as a Streamlined Approach to Noninvasive Breast Cancer Imaging. Fort Belvoir, VA: Defense Technical Information Center, Dezember 2013. http://dx.doi.org/10.21236/ada596735.
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Farkas, Michelle E. Chemically Modified Bacteriophage as a Streamlined Approach to Noninvasive Breast Cancer Imaging. Fort Belvoir, VA: Defense Technical Information Center, Oktober 2011. http://dx.doi.org/10.21236/ada556023.
