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Auswahl der wissenschaftlichen Literatur zum Thema „Bacterial phytase“
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Zeitschriftenartikel zum Thema "Bacterial phytase"
1
Chuiko, N. V., A. Yu Chobotarov und I. K. Kurdish. „Growth and Phytase Activities of Bacillus subtilis IMV B-7023 During Cultivation with Sodium Phytate“. Mikrobiolohichnyi Zhurnal 83, Nr. 6 (17.12.2021): 13–19. http://dx.doi.org/10.15407/microbiolj83.06.013.
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Bacteria of the genus Bacillus are known for their ability to mineralize organic phosphorus compounds. Phytates constitute up to 60–80% of the total plant phosphorus and almost 50% of soil organic phosphorus. Phytates phosphorus is unavailable for plants. Bacillus can synthesize phosphatases both wide spectrum of action, and highly specific phytases that catalyze the hydrolysis of phytates. Therefore, the aim of this work was to study the growth and phytase activity of Bacillus subtilis IMV B-7023, which is the component of the ”Azogran” complex bacterial preparation for plant growing. Methods. The growth activity of bacteria was studied by cultivation methods, the phytase activity – by measuring the amount of phosphate released from sodium phytate during the enzymatic reaction. Results. It was shown that B. subtilis IMV B-7023 assimilated phytate as the source of phosphorus nutrition during cultivation in media with 0.5, 1.0 and 2.0 g/L of sodium phytate. The highest growth activity of these bacteria was observed after two days of cultivation in medium with 1.0 g/L of phytate. The number of bacteria was (3.91±0.32)×109 CFU/mL under these conditions. At the same time, B. subtilis IMV B-7023 demonstrated a low level of phytate assimilation as a source of carbon nutrition. Thus, after two days of cultivation the number of bacteria increased from (4.12±0.09)×106 CFU/mL to (1.07±0.07–3.11±0.51)×107 CFU/mL in the presence of 0.5–2.0 g/L phytate in the medium and the absence of another carbon source. It was determined that strain B. subtilis IMV B-7023 had phytase activity, the highest activity (221.85±0.12 U/g) was on the first day of their cultivation in medium with inorganic phosphates. It should be noted that B. subtilis IMV B-7023 phytase activity was lower during cultivating in medium with sodium phytate as a source of phosphorus nutrition, than in medium with inorganic phosphates. The obtained fact may be due to phytate hydrolysis by widespecific phosphatases. Higher rates of phytase activity obtained on the first and third days compared to the second and fourth days of bacterial cultivation may indicate the expression of phosphatases genes only in the period required for maximum bacterial development, in the absence of these proteins in the media. At the same time, the phytase activity of B. subtilis IMV B-7023 after 2 days cultivation in a media with 0.5 and 1.0 g/L of sodium phytate (194.80±0.15 U/g and 160.90±0.13 U/g, respectively) as the source of carbon and phosphorus was higher compared to the activity of bacteria on medium with inorganic phosphates (137.79±0.10 U/g). This may be caused by the synthesis of a larger number of highly specific phosphatases (phytases) in bacterial cells at the presence of only phytate in the medium as a substrate. Conclusions. B. subtilis IMV B-7023 strain is characterized by growth on nutrient medium with sodium phytate and phytase activity. Because they are soil microorganisms used as the component of the ”Azogran” complex bacterial preparation for plant growing, the ability to hydrolyze and assimilate phytate is important for functioning of this strain in the rhizosphere. The obtained results extend the understanding of B. subtilis IMV B-7023 influence on phosphorus nutrition and development of plants.
2
Williams, P. J., und T. G. Taylor. „A comparative study of phytate hydrolysis in the gastrointestinal tract of the golden hamster (Mesocricetus auratus) and the laboratory rat“. British Journal of Nutrition 54, Nr. 2 (September 1985): 429–35. http://dx.doi.org/10.1079/bjn19850128.
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1. The role of bacterial, dietary and intestinal phytases (EC 3. 1. 3. 8) in the hydrolysis of phytate was investigated in the golden hamster and rat by assaying phytase in the small intestine and by measuring the disappearance of phytate from the stomach and large intestine, using chromium oxide as an insoluble solid-phase marker.2. It was confirmed that an active phytase was present in the proximal third of the small intestine of the rat but the enzyme was undetectable in the hamster.3. Extensive bacterial breakdown of phytate occurred in the pregastric pouch and true stomach of the hamster with both phytase-containing and phytase-free diets, with phytate digestibilities in the true stomach ranging from 0.69–0.90, confirming that the hamster can be regarded as a pseudo-ruminant.4. With a phytase-free diet, the digestibility of phytate in the stomach of the rat was very low (0.05) but with a wheat-based diet substantial breakdown of phytate occurred (digestibility up to 0.49), presumably under the influence of the cereal phytase.5. Intestinal phytase did not appear to be of great significance in the rat but some further hydrolysis of the residual phytate probably occurred in the large intestine of both species by bacterial phytase.
3
Moss, Amy F., Sonia Yun Liu und Peter H. Selle. „Progress in comprehending the phytate–phytase axis in chicken-meat production“. Animal Production Science 58, Nr. 10 (2018): 1767. http://dx.doi.org/10.1071/an17594.
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After an extended delay, the level of acceptance of exogenous phytases by the global chicken-meat industry is now almost complete. Contemporary bacterial phytases degrade phytate primarily in the gizzard. The extent of phytate degradation determines the extent to which phytate-bound phosphorus (P) is liberated; however, studies designed to investigate phytate degradation along the digestive tract have generated some confusing outcomes. This may be related to the reactivity of the phytate moiety, coupled with problems with inert dietary markers and perhaps a lack of complete and uniform extractions of phytate from digesta due to variations in digesta pH and phytate solubility. Quite recently, phytase was shown to have profound impacts on sodium (Na) digestibility coefficients in four segments of the small intestine. This has obvious implications for intestinal uptakes of glucose and amino acids via their respective Na+-dependent transport systems and it is possible that phytate and phytase have reciprocal impacts on ‘sodium pump’ (Na+, K+-ATPase) activity. It has been recently demonstrated unequivocally that phytase has the capacity to increase amino acid digestibility coefficients to the extent that phytase may generate a ‘proximal shift’ in the sites of amino acid absorption. The impact of phytase on starch digestibility is more equivocal and phytase responses may stem more from enhanced glucose absorption rather than starch digestion. The acceptance of phytase is hardly surprising, given its capacity to increase P utilisation coupled with numerous other positive influences that are still being properly realised.
4
Chuiko, N. V., A. Yu Chobotarov und I. K. Kurdish. „Abiotic Factors Influence on Bacillus subtilis IMV B-7023 Phytase Activity“. Mikrobiolohichnyi Zhurnal 84, Nr. 6 (28.02.2023): 3–9. http://dx.doi.org/10.15407/microbiolj84.06.003.
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Bacteria of the Bacillus genus can synthesize specific phytase enzymes. This property is especially important for soil bacteria. It helps to mineralize phytin and phytates and to provide these bacteria and plants (in the root zone of which they live) with the available phosphorus. Our previous studies have demonstrated that the Bacillus subtilis IMV B-7023 strain exhibits a phytase activity and can use phytate as a nutrition source. It is a component of the «Azogran» complex bacterial preparation for crop production. As known, abiotic environmental factors can influence the phytase activity of bacteria. In particular, the phytase activity changes significantly under different pH and temperatures. Solid soil particles, including nanosized minerals, can also influence bacteria’s enzymatic activity. The influence of abiotic factors on Bacillus subtilis IMV B-7023 phytase activity has not previously been studied, so this was the aim of our research. Methods. The phytase activity of bacteria was studied by measuring the amount of phosphate released from sodium phytate during the enzymatic reaction, and the nanomaterials’ influence on growth — by cultivation methods. Results. The highest B. subtilis IMV B-7023 phytase activity was observed at 28°C. Also, there was no B. subtilis IMV B-7023 phytase activity at pH 4—6. However, this activity increased at pH 7 and did not change significantly with increasing the buffer system pH to 12. Silicon dioxide influence on the B. subtilis IMV B-7023 growth activity during cultivation in a media with phytate as a phosphorus source depended on the nanomaterial concentration. Thus, at 0.05 and 0.5 g/L of silicon dioxide in the medium, this strain growth activity increased by 8—18%, and at 5.0 g/L of these nanoparticles, bacteria growth inhibition by 19% was observed. At the same time, clay mineral bentonite did not affect the B. subtilis IMV B-7023 growth under the studied cultivation conditions. In addition, silicon dioxide and bentonite stimulated B. subtilis IMV B-7023 phytase activity at all studied concentrations. So, phytase activity increased by 1.82—3.34 times upon adding silicon dioxide and by 2.54—5.83 times upon adding bentonite into the medium. Since the optimal values for phytase activity of most genus Bacillus bacteria are within neutral pH values and temperatures within 50—55°C, a property of B. subtilis IMV B-7023 to show maximum phytase activity at alkaline pH and lower temperatures (28°C) and also stimulation of this activity by soil minerals increases competitiveness of this strain as a component of a bacterial preparation for crop production. Conclusions. Abiotic environmental factors influence the B. subtilis IMV B-7023 supernagrowth and phytase activity. Optimal physicochemical factors for the phytase activity of these bacteria are temperature 28°C and pH 7—12. The concentrations 0.05, 0.5, and 5.0 g/L of silicon dioxide and bentonite increase B. subtilis IMV B-7023 phytase activity. The effect of these nanoscale minerals on the B. subtilis IMV B-7023 growth depends on their type and concentration during cultivation in the medium with phytate as a phosphorus source. The obtained results indicate the potential ability of the B. subtilis IMV B-7023 strain to effectively assimilate phytates in neutral and alkaline soils, especially due to the interaction of these bacteria with bentonite and silicon dioxide nanoparticles. This expands the possibility of using B. subtilis IMV B-7023 in agricultural technologies.
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Menezes-Blackburn, Daniel, Nahad Al-Mahrouqi, Buthaina Al-Siyabi, Adhari Al-Kalbani, Ralf Greiner und Sergey Dobretsov. „Bacterial Communities Associated with the Cycling of Non-Starch Polysaccharides and Phytate in Aquaponics Systems“. Diversity 13, Nr. 12 (30.11.2021): 631. http://dx.doi.org/10.3390/d13120631.
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Aquaponics are efficient systems that associate aquatic organisms’ production and plants by recirculating water and nutrients between aquaculture and hydroponic tanks. In this study, we characterised the bacterial communities in the freshwater aquaponics system that can mineralise polysaccharides and phytate by producing carbohydrate-degrading enzymes and phytases, by 16S rRNA gene sequencing and in vitro culture techniques. Around 20% of the operational taxonomic units (zOTUs) identified were previously reported to carry fibre-degrading enzyme putative genes, namely β-glucanase (1%), xylanase (5%), or cellulases (17%). Ten % of the zOTUs were previously reported to carry putative genes of phytases with different catalytic mechanisms, namely β-propeller (6%), histidine acid phytases (3%), and protein tyrosine phytase (<1%). Thirty-eight morphologically different bacteria were isolated from biofilms accumulated in fish and plant compartments, and identified to belong to the Bacilli class. Among these, 7 could produce xylanase, 8 produced β-glucanase, 14 produced cellulase, and 11 isolates could secrete amylases. In addition, Staphylococcus sp. and Rossellomorea sp. could produce consistent extracellular phytate-degrading activity. The PCR amplification of β-propeller genes both in environmental samples and in the isolates obtained showed that this is the most ecologically relevant phytase type in the aquaponics systems used. In summary, the aquaponics system is abundant with bacteria carrying enzymes responsible for plant-nutrient mineralisation.
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Suleimanova, Aliya, Daria Bulmakova und Margarita Sharipova. „Heterologous Expression of Histidine Acid Phytase From Pantoea sp. 3.5.1 in Methylotrophic Yeast Pichia Pastoris“. Open Microbiology Journal 14, Nr. 1 (30.07.2020): 179–89. http://dx.doi.org/10.2174/1874285802014010179.
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Background and Objective: The major storage form of phosphorus in plant-derived feed is presented by phytates and not digested by animals. Phytases are able to hydrolyze phytates and successfully used as feed additives. Nevertheless, nowadays, there is a constant search of new phytases and expression systems for better production of these enzymes. In this study, we describe cloning and expression of gene encoding histidine acid phytase from Pantoea sp. 3.5.1 using methylotrophic yeast Pichia pastoris as the host. Methods: The phytase gene was placed under the control of the methanol-inducible AOX1 promoter and expressed in P. pastoris. Experiments of small-scale phytase expression and activity assays were used to test recombinant colonies. Four different signal peptides were screened for better secretion of phytase by P. pastoris. After 36 h of methanol induction in shake flasks, the maximum extracellular phytase activity (3.2 U/ml) was observed in P. pastoris strain with integrated construct based on pPINK-HC vector and Kluyveromyces maxianus inulinase gene signal sequence. This phytase was isolated and purified using affinity chromatography. Results: Recombinant phytase was a glycosylated protein, had a molecular weight of around 90 kDa and showed maximum activity at pH 4.0 and at 50°C. Recombinant phytase had excellent thermal stability – it retained high residual activity (100% ± 2%) after 1 hour of heat treatment at 70°C. Conclusion: The enhanced thermostability of the recombinant phytase, its expression provided by strong inducible promotor and the effectively designed expression cassette, the simple purification procedure of the secreted enzyme, and the possibility of large-scale expression make the foundation for further production of this bacterial phytase in P. pastoris at an industrial scale.
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Gauchan, Dhurva Prasad, Shishir Pandey, Bikash Pokhrel, Nabin Bogati, Puja Thapa, Ashesh Acharya, Bishnu Maya KC und Janardan Lamichhane. „Growth Promoting Role of Phytase Producing Bacteria Isolated from Bambusa tulda Roxb. Rhizosphere in Maize Seedlings Under Pot Conditions“. Journal of Nepal Biotechnology Association 4, Nr. 1 (22.03.2023): 17–26. http://dx.doi.org/10.3126/jnba.v4i1.53442.
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Phytase enzymes have focused on their role in agriculture to generate bioavailable phosphorus (P) requires for plant nutrition. Herein, the feasibility of phytase-producing microbes as biofertilizers was studied. Bacteria with greater potential for hydrolysing calcium phytate based on the halo-to-colony ratio from Bambusa tulda Roxb. rhizosphere was isolated. Phytase activity by incorporating wheat bran, phytase screening and Luria Bertani (LB) medium after acetone precipitation was measured. Bacterial genomes were screened for the presence of β-propeller phytase gene corresponding to the Bacillus spp. using polymerase chain reaction (PCR). Finally, the effect of the isolates on the growth of maize seedlings under pot conditions in P-deficient loamy soil was evaluated. Ten distinct bacterial isolates collected from B. tulda rhizosphere were capable of mineralizing phytate and the maximum effect was observed for designated SRBR-04. Most isolates solubilized Ca3(PO4)2 as a sole P source in Pikovskaya’s agar. Five isolates selected for the study synthesized auxin in the LB broth supplemented with 1 mg mL-1 L-Tryptophan (1.63 to 4.5 μg mL-1). Phytase production was highest in wheat bran with isolate SRBR-04 producing a maximum of 0.34 U mg-1. Two isolates (SRBR-01, SRBR-04) screened positive for the presence of Bacillus phytase gene. Pot assay in P-deficient soil showed significant (p < 0.05) biomass promotion for the isolate SRBR-07 in shoot height (57%), dry shoot weight (178%), dry root weight (104%) and leaf area (113%) over the untreated control. Amendment of P-deficient agricultural soils with phytase-producing bacteria would provide a sustainable approach for P nutrition management in Zea mays.
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Christensen, Trine, Yueming Dersjant-Li, Vincent Sewalt, Rie Mejldal, Svend Haaning, Sina Pricelius, Igor Nikolaev, Robin A. Sorg und Arno de Kreij. „In Vitro Characterization of a Novel Consensus Bacterial 6-Phytase and One of its Variants“. Current Biochemical Engineering 6, Nr. 3 (28.12.2020): 156–71. http://dx.doi.org/10.2174/2212711906999201020201710.
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Background:: Microbial phytases are added to animal feed to hydrolyze phytic acid (myoinositol hexakisphosphate, IP6) and phytate (salt of phytic acid) increasing phosphorus bioavailability. Novel phytases with enhanced bio-efficacy are being developed. Objective:: To characterize the biochemical and enzymatic properties of a novel consensus bacterial 6- phytase and its variant (PhyG), produced in Trichoderma reesei. Methods:: The in vitro specific activity, kinetic parameters, pH-activity profiles (relative to pH5.5), IP6 degradation, hydrolysis products and phosphate release of the phytases were determined using sodium phytate substrate. Melting point (Tm) was determined by differential scanning calorimetry and thermostability assessed by measuring residual activity at different temperatures. In vivo effects of PhyG supplementation at 0 to 1,000 FTU/kg on ileal IP6 digestibility and IP ester concentrations were determined in piglets. Results:: Both phytases exhibited pH optima of 3.5-4.5, high relative activity over a wide pH range (pH2.0-5.0), and substantial relative activity at pH1.5. At pH3.0, the specific activity of the PhyG variant was 1487 U/mg protein and at pH3.5 the kinetic constants were 240 μM (Km) and 1873 s-1 (Kcat). The hydrolysis of IP6 by both phytases was rapid. The major initial hydrolysis product was DLI( 1,2,3,4,5)P5, designating the phytases as bacterial 6-phytases (EC 3.1.3.26). Hydrolysis occurred at the D-3 (L-1) position in ~30% of instances, indicating a dual-specificity. Conclusion: Both phytases showed high thermostability compared to wild type and existing commercial bacterial 6-phytases; PhyG exhibited 95% residual activity after 20 min incubation at 85.4ºC (pH5.5), Tm50 of ~93.2ºC and Tm of 98.8ºC. In vivo, PhyG at 1,000 FTU/kg achieved an ileal digestibility of IP6 of 89.3%.
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Espinosa, Charmaine D., Deepak E. Velayudhan, Yueming Dersjant-Li und Hans H. Stein. „60 Effect of a Novel Consensus Bacterial 6-phytase Variant on Mineral Digestibility and Bone Ash in Young Growing Pigs Fed Diets with Different Concentrations of Phytate“. Journal of Animal Science 99, Supplement_1 (01.05.2021): 45–46. http://dx.doi.org/10.1093/jas/skab054.079.
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Abstract An experiment was conducted to test the hypothesis that increasing levels of phytase increases mineral digestibility and bone ash by pigs fed diets containing 0.23%, 0.29%, or 0.35% phytate-P. Within each level of phytate, 5 diets were formulated based on corn, soybean meal, and canola meal to contain 0, 500, 1,000, 2,000, or 4,000 phytase units (FTU)/kg of a novel consensus bacterial 6-phytase variant (PhyG). In addition, 3 reference diets were formulated by adding a commercial Buttiauxella phytase (PhyB) at 1,000 FTU/kg to the 3 diets containing no PhyG. A randomized complete block design with 144 pigs (12.70 ± 4.01 kg), 18 diets, and 8 replicate pigs per diet was used. Pigs adapted to diets for 15 d followed by 4 d of fecal collection. Femurs were collected on the last day. Data were analyzed as a 3 × 5 factorial with 3 levels of phytate-P and 5 levels of phytase, and contrast statements were used to compare 1,000 FTU of PhyG with PhyB. Pig was the experimental unit. Diets containing 0.35% phytate-P had reduced (P < 0.01) apparent total tract digestibility (ATTD) of Ca, P, Mg, and K compared with diets containing 0.23% or 0.29% phytate-P, but inclusion of phytase increased (P < 0.01) ATTD of Ca, Na, and K (Table 1). Phytase increased ATTD of P and Mg, but to a greater extent in diets with 0.23 or 0.29% phytate-P than in diets with 0.35% phytate-P (interaction, P < 0.05). Phytase increased bone ash, but to a greater extent if there was 0.35 rather than 0.23 or 0.29% phytate-P in the diets (interaction, P < 0.05). PhyG increased ATTD of P more (P < 0.05) than PhyB. In conclusion, the novel consensus phytase is effective in increasing bone ash and ATTD of Ca, P, Na, Mg and K.
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Suleimanova, Aliya D., Astrid Beinhauer, Liia R. Valeeva, Inna B. Chastukhina, Nelly P. Balaban, Eugene V. Shakirov, Ralf Greiner und Margarita R. Sharipova. „Novel Glucose-1-Phosphatase with High Phytase Activity and Unusual Metal Ion Activation from Soil Bacterium Pantoea sp. Strain 3.5.1“. Applied and Environmental Microbiology 81, Nr. 19 (24.07.2015): 6790–99. http://dx.doi.org/10.1128/aem.01384-15.
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ABSTRACTPhosphorus is an important macronutrient, but its availability in soil is limited. Many soil microorganisms improve the bioavailability of phosphate by releasing it from various organic compounds, including phytate. To investigate the diversity of phytate-hydrolyzing bacteria in soil, we sampled soils of various ecological habitats, including forest, private homesteads, large agricultural complexes, and urban landscapes. Bacterial isolatePantoeasp. strain 3.5.1 with the highest level of phytase activity was isolated from forest soil and investigated further. ThePantoeasp. 3.5.1agpPgene encoding a novel glucose-1-phosphatase with high phytase activity was identified, and the corresponding protein was purified to apparent homogeneity, sequenced by mass spectroscopy, and biochemically characterized. The AgpP enzyme exhibits maximum activity and stability at pH 4.5 and at 37°C. The enzyme belongs to a group of histidine acid phosphatases and has the lowestKmvalues toward phytate, glucose-6-phosphate, and glucose-1-phosphate. Unexpectedly, stimulation of enzymatic activity by several divalent metal ions was observed for the AgpP enzyme. High-performance liquid chromatography (HPLC) and high-performance ion chromatography (HPIC) analyses of phytate hydrolysis products identifydl-myo-inositol 1,2,4,5,6-pentakisphosphate as the final product of the reaction, indicating that thePantoeasp. AgpP glucose-1-phosphatase can be classified as a 3-phytase. The identification of thePantoeasp. AgpP phytase and its unusual regulation by metal ions highlight the remarkable diversity of phosphorus metabolism regulation in soil bacteria. Furthermore, our data indicate that natural forest soils harbor rich reservoirs of novel phytate-hydrolyzing enzymes with unique biochemical features.
Dissertationen zum Thema "Bacterial phytase"
1
Pal, Roy Moushree. „Isolation, characterization, molecular gene cloning and expression of novel bacterial phytase from environmental samples“. Thesis, University of North Bengal, 2015. http://hdl.handle.net/123456789/2744.
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2
Olstorpe, Matilda. „Feed grain improvement through biopreservation and bioprocessing : microbial diversity, energy conservation and animal nutrition aspects /“. Uppsala : Dept. of Microbiology, Swedish University of Agricultural Sciences, 2008. http://epsilon.slu.se/200877.pdf.
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3
Yang, Shu-Hui, und 楊舒卉. „Screening lactic acid bacteria with phytase activity from fish intestine for the phytate degradation of soybean“. Thesis, 2015. http://ndltd.ncl.edu.tw/handle/vc5u54.
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4
Yi, Yang Mu, und 楊慕義. „Establishment of optimal conditions for the degradation of phytate in soybean meal by lactic acid bacteria with phytase activity using a novel solid-state fermenter“. Thesis, 2016. http://ndltd.ncl.edu.tw/handle/60706240763577538750.
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碩士國立臺灣海洋大學
食品科學系
104
The consumption of fish meal is raising because of the booming development of aquaculture. Soybean meal with stable supplement and high protein content is the best substitute for fish meal in aquaculture feed. However, soybean contains many antinutrient factors such as phytate and saponin, which can block the nutrient absorption of the organism and cause the adverse effect on fish growth. In this study Lactobacillus plantarium FPS 2520 with phytase activity was used to degrade phytate in soybean and soybean meal using a solid-state fermentation in the flask. The effects of soybean particle size and water content of substrate on the phyate and saponin degradation were evaluated first. The phytate degradation efficacy increased with decreasing size of soybean particle, with the powder form being most favored. The best ratio of soybean powder/soybean meal to water for phytate degradation is 1:3; while the water content did not affect the saponin degradation. The residual saponin content was around 1.8 to 2.0 mg/g after FPS 2520 fermentation at 37oC for 72 h, irrespective the water content of substrate and soybean particle size. After fermentation of soybean (20.23 ± 0.26 mg/g phytate) and soybean meal (54.68 ± 0.42 mg/g phytate) by FPS 2520 with an initial inoculum of 6 log CFU/mL at 37oC for 72 hr, the phytate degradation efficacy were 57.92% and 17.06 %, respectively. The phytate degradation efficacy in soybean and soybean meal was further increased to 75 and 34.56% by using two stages of incubation temperatures of 37oC for 24 h, followed by 50oC for 24 h.Using a novel solid-state fermenter to try to increase the phytate degradation efficacy in soybean meal by implication of the systems of rotation and ventilation, and also by the strategy of two incubation temperature. Factors of rotation (0, 1, 2 rpm) and ventilation (0, 0.5, 1.0, 1.5, 2.0 vvm) on phytate degradation were evaluated first. Rotation with 2 rpm significantly increased the phytate degradation efficacy from 51.9 % to 61.3 % by FPS 2520 at 37oC for 72 h; while it did not affect saponin degradation efficacy (about 65.57%). Ventilation at 1.5 vvm did not affect bacterial growth; while phytate degradation efficacy was increased from 13.56% to 38.83%. The effects of rotation and ventilation on phytate degradation were further investigated using the stratagem of 2 incubation temperatures of 37oC and 50oC. The phytate degradation efficacy in soybean meal by FPS2520 was further increased to 97.60 % using two stages of incubation temperatures of 37oC at 2 rpm and 1.5 vvm for 24 h, followed by 50oC at 2 rpm 0.5 vvm for 24 h. After fermentation the residual phytate and saponin in soybean meal was only 1.16 ± 3.32 mg/g and 81.25 ± 7.53 mg/g.
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CHEN, CHI-CHUN, und 陳智群. „The Selection and Application of Phytase Producing Lactic Acid Bacteria“. Thesis, 2019. http://ndltd.ncl.edu.tw/handle/r5559c.
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碩士國立澎湖科技大學
食品科學系碩士班
107
127 strains of lactic acid bacteria with phytase activity were screened from 614 strains of lactic acid bacteria isolated from the algae in Penghu keeping in our laboratory. Among them, 7 strains of the highest activity lactic acid bacteria were identified as 3 strains of Lactobacillus plantarum (5-1, 1.2U/mL; 20-4-1, 1.7U/mL; 62-3, 1.72U/mL) , 1 strain of Lactobacillus brevis (12-5, 1.88 U/mL), 1 strain of Lactobacillus rhamnosus (128-1, 1.24 U/mL) and 2 strains of Lactobacillus fermentum (62-6, 0.91 U/mL; 366-1, 1.37 U/mL). After fermenting with phytase producing LAB, phytic acid in brown rice pulp, shelled wheat pulp, and whole soy milk medium can be degraded effectively. The pH was significantly reduced to 5 after 12 hours of fermentation. The number of bacteria in the fermentation for 24 hours reached a maximum. The phytic acid degradation gradually slowed down after 48 hours of fermentation. Comparing the number of viable bacteria and the amount of phytic acid degradation， Lactobacillus plantarum 20-4-1 is the best strain which can be used in grain fermentation, the phytic acid residue after fermentation for 72 hours were 13.76 mg/g (36%) of whole soybeans, 2.56 mg/g of brown rice (28%), and 5.63 mg / g (50.0%) of the shelled wheat.
Buchteile zum Thema "Bacterial phytase"
1
Bianchi, Thomas S., und Elizabeth A. Canuel. „Lipids: Hydrocarbons“. In Chemical Biomarkers in Aquatic Ecosystems. Princeton University Press, 2011. http://dx.doi.org/10.23943/princeton/9780691134147.003.0010.
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This chapter examines hydrocarbons present in the environment and derived from natural and anthropogenic sources. Since the industrial revolution, the abundance of hydrocarbons derived from anthropogenic sources (petroleum hydrocarbons) has increased significantly in aquatic systems. Natural oil seeps and erosion of bitumen deposits can also contribute to hydrocarbon abundance and composition in systems. These petroleum hydrocarbons can be distinguished from biological hydrocarbons by their absence of odd-carbon chain lengths commonly found in biological hydrocarbons and the greater structural diversity found in petroleum hydrocarbons. The chapter focuses on naturally produced hydrocarbons. It provides examples of how aliphatic and isoprenoid hydrocarbons have been successfully used to distinguish between algal, bacterial, and terrigenous vascular plant sources of carbon in aquatic systems. It discusses how pristine and phytane are formed from phytol under oxic vs. anoxic conditions, respectively. It also introduces highly branched isoprenoids and their use as algal biomarkers.
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Sharma, Neha. „Role of phytases from lactic acid bacterial species in level upgradation of bioavailable micronutrients in food applications“. In Lactic Acid Bacteria as Cell Factories, 219–37. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-323-91930-2.00011-0.
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3
Kumar, Vikas, Md Sakhawat Hossain, Janice A. Ragaza und Marina Rubio Benito. „The Potential Impacts of Soy Protein on Fish Gut Health“. In Soybean for Human Consumption and Animal Feed. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.92695.
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Soy protein is the major source of protein as fishmeal replacement in fish feed because of its worldwide availability and low price. However, the presence of high carbohydrate content along with saponins, lectins, and phytates can have a negative impact on fish gut health. Based on the literature and our lab studies, dietary soybean meal can cause a dose-dependent type of distal intestine inflammation called enteritis in commercial fish species including salmonids. This leads to reduced absorptive capacity, increased mucus secretion, hyperpermeability, and leucocyte infiltration in the lamina propria and submucosa, also inducing the pro-inflammatory cytokine genes expression, including Il-1β, Il-8, and Tnf-α. In addition, dietary soy may alter the composition and population of the gut microbiota via providing nutrients and energy that preferentially support the growth of some gut bacteria. This chapter summarizes the current knowledge of the effects of soy protein on the enteritis and gut microbiota.
Konferenzberichte zum Thema "Bacterial phytase"
1
„Phytase Activity of Four Endophytes Bacteria from Zea Mays L.“ In April 17-18, 2018 Kyoto (Japan). International Institute of Chemical, Biological and Environmental Engineering, 2018. http://dx.doi.org/10.17758/iicbe1.c0418153.
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2
Damayanti, Ema, Febiyani Ndaru Ratisiwi, Lusty Istiqomah, Langkah Sembiring und Andi Febrisiantosa. „Phytate degrading activities of lactic acid bacteria isolated from traditional fermented food“. In PROCEEDINGS FROM THE 14TH INTERNATIONAL SYMPOSIUM ON THERAPEUTIC ULTRASOUND. Author(s), 2017. http://dx.doi.org/10.1063/1.4978126.
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3
Kumar Puri, Adarsh, Johnson Zininga, Kugenthiren Permaul und Suren Singh. „A thermo-acid-stable and protease-resistant phytase from a newly isolated thermophilic bacterium, Bacillus ginsengihumi“. In Annual International Conference on Advances in Biotechnology. Global Science & Technology Forum (GSTF), 2015. http://dx.doi.org/10.5176/2251-2489_biotech15.68.
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