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Zeitschriftenartikel zum Thema "Bacteria isolated"
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Mawardi, Mira, Agustin Indrawati, Angela Mariana Lusiastuti und I. Wayan Teguh Wibawan. „CHARACTERIZATION OF SPORE-FORMING BACTERIA ISOLATED FROM TILAPIA (OREOCHROMIS NILOTICUS) AND THEIR POTENTIAL FOR A PROBIOTIC CANDIDATE“. Indonesian Aquaculture Journal 18, Nr. 2 (19.12.2023): 105. http://dx.doi.org/10.15578/iaj.18.2.2023.105-114.
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Gram-positive spore bacteria are widely used as probiotics in general sectors. However, there are still limited bacterial isolates as probiotic candidates available from indigenous isolates, especially in aquaculture. This study aimed to obtain potential spore-forming isolates as probiotic candidate for tilapia. Tilapia fish samples were collected from Sukabumi, Ciamis, Serang, and Papua. Bacterial isolates were isolated from the digestive tract of tilapia. Bacteria were identified based on their morphological, molecular characteristics, complete genome composition, and cell surface identification based on hydrophobic properties. In this study, six bacteria were isolated and identified by molecular characteristics using 16S rRNA sequences. Based on the phylogenetic analysis, the 9 PP isolate was Priestia megaterium basonym: Bacillus megaterium, CMS 16N isolate was Brevibacillus halotolerans, PPN 10 isolate was Bacillus sp., 3.1 SKBM isolate was Bacillus mycoides, CMS 22 N and SRG32 isolate were Bacillus subtilis. Six bacteria had different phenotypicals, ATGC sequence compositions, and a higher proportion of total G~C sequence composition above 50%. The coherent cell surface hydrophobicity test was positive on the SAT, SA, AA, and compact growth patterns in soft-agar media for 9 PP, CMS 22 N, and SRG32 isolates. From our study, the indigenous spore-forming bacteria isolated from tilapia stomachs are enzymatic bacteria, which have a strong attachment to host tissue and high potential as a probiotic candidate for fish. Various hydrophobicity test results from each isolate indicate that the protein composition in the cell surface is different.
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Amelia, Titik Fadilah, Ace Baehaki und Herpandi Herpandi. „Aktivitas Reduksi Merkuri pada Bakteri yang Diisolasi dari Air dan Sedimen di Sungai Musi“. Jurnal FishtecH 5, Nr. 1 (25.09.2016): 94–106. http://dx.doi.org/10.36706/fishtech.v5i1.3522.
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This study aims to isolate mercury resistant bacteria from water and sediment in Pulau Salah Nama which located on the River Musi Palembang, characterized mercury resistant bacteria growing at the highest concentration of HgCl2 and test the power of these bacteria in reducing mercury. The research was conducted from June 2015 until September 2015 using experimental methods laboris and descriptive data analysis. Reasecrh consissted of several stages, including sampling, bacterial isolation, characterization to determine the type of bacteria and mercury reducing power of bacteria. Isolation of bacteria produced 10 isolates. A1 was bacteria isolated from water and A2 was bacteria isolated from sediment. From 10 isolates selected 2 isolates of the higest concentration of HgCl2. Selected isolate A1 have the lowest mercury reduction power of 39.26% and selected isolate A2 have the highest mercury reduction power of 65.93%. In control medium without inoculant a decline in mercury concentration of 39.44%. Based on the characterization of the bacteria biochemical activity known A1 and A2 were Bacillus subtilis
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Reshetnikov, M. V., und V. P. Patyka. „Bacteria-antagonists of the agents of soryz bacterial diseases“. Agricultural Science and Practice 10, Nr. 3 (28.02.2024): 46–60. http://dx.doi.org/10.15407/agrisp10.03.046.
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Aim. To isolate and identify bacteria with antagonist properties for biocontrol of the agents of bacterial diseases of soryz (Sorghum oryzoidum) and sorghum crops. Methods. The studies were conducted in 2021-2023. Spore-forming bacteria were isolated from the soryz samples, collected in the fields of the experimental farm of the Uman National Horticulture University (Cherkasy region, Uman). Lactic acid bacteria were isolated from soryz plants, collected in the private land plot, located between the villages of Teolyn, Vladyslavchyk, Kniazhyky in Monastyryshche com- munity, Uman district, where Pershotravneve hamlet used to be situated. A total of 1,250 samples were analyzed. The experiment had three repeats. Spore-forming and lactic acid bacteria were isolated from the surface of soryz plants on the firm ripe stage in summer while isolating phytopathogenic bacteria. The isolates of lactic acid bacteria- antagonists were also isolated from the inner part of winter stubble stalk of soryz, collected from the tilled field. The antagonistic activity of the strains of lactic acid bacteria and spore-forming bacteria, isolated from different ecological niches, to phytopathogens of soryz and sorghum crops was determined in vitro. The strains of Pseudomonas syringae, the agents of soryz bacterial spots, were used as test-cultures: 211141a, 211141, 210341, 21034, and 210521, along with the collection strains of phytopathogens: Pseudomonas syringae 8299, Pseudomonas syringae subsp. syringae UKM B-1021, X. oryzae 8375, Dickeya chrysanthemi 8683, Diskeya chryzanthemi 8683. The antagonistic activity of the extracted isolates of spore-forming and lactic acid bacteria was studied using the method of radial strokes (joint cultivation of the antagonist and the strains under investigation). The bacterial isolates were deemed inactive if the growth delay zone was 0–5 mm (–), from 5 to 10 mm (+) – low activity, 11–20 mm (++) – moderate activity, over 20 mm (+++) – high activity regarding the test-cultures. To check the effect of the isolate-antagonist of phytopathogenic bacteria, artificial infecting was conducted in the field conditions. For this purpose, a diurnal culture of the antagonist was introduced into the stalk of plants in the concentration of 1×108 colony-forming units, and 24 h later, a culture of test-strain of the phytopathogen was administered above the previous puncture. The results were evaluated 7–14 days after the artificial infection. The experiment had three repeats. The isolates of bacteria which demonstrated their an- tagonistic properties regarding the phytopathogenic bacteria were identified by their morphological properties, Gram staining, catalase test, profile of carbohydrate fermentation and mass-spectrometry (MALDI-TOF – Matrix Assisted Laser Desorption/Ionization) using VITEK MS mass-spectrometer. Results. Thirty-eight spore-forming bacterial iso- lates were extracted from soryz; among these, 21030, 21095, 21040, ASV1, ASV3, B4 demonstrated their antagonistic activity towards the investigated phytopathogenic bacteria. Isolate 21040 showed high antagonistic activity to most test-strains of P. syringae from soryz (the zone of negative culture – 23–30 mm) and lower activity regarding the collection cultures. Isolates B4 and AVS3 demonstrated their selective activity regarding the investigated phytopatho- gens. Twenty isolates of lactic acid bacteria were extracted. Higher antagonistic activity was noted for the isolates of lactic acid bacteria 8/1 and F1 to the strains of P. syringae, isolated from soryz and collection cultures. The highest antagonistic activity of isolate 8/1 was noted regarding test-strains of P. syringae 210521 and X. oryzae 8375 (the zone of negative culture – 40–35 mm). In the field conditions, the treatment of sorghum plants with F1 affected the pathological process that developed due to the impact of the phytopathogenic bacteria P. syringae, which led to the reduction in disease symptoms. The taxonomic position of the isolates of bacteria, which seem to be promising for the control of disease agents, was determined. In terms of morphology of cells and colonies, the biochemical profile, and mass-spectrometry MALDI-TOF, the spore-forming isolates 21040 and B4 were identified as Bacillus subtilis, and ASV3 – as Bacillus vallismortis. The identified isolates of lactic acid bacteria were Lactobacillus pentosus F1 and Lactobacillus sakei 8/1. Conclusions. In addition to phytopathogenic bacteria, from soryz plants we isolated the strains of spore-forming bacteria Bacillus subtilis 21040, B4, Bacillus vallismortis AVS3 and such lactic acid bacteria as Lactiplantibacillus pentosus and Lactobacillus sakei 8/1 (Latilactobacillus sakei 8/1), promising for the elaboration of methods for the biocontrol of the agents of bacterial diseases.
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Msango Soko, K. R., R. C. Bhattacharya, B. Ramakrishnan, K. Sharma und S. Subramanian. „Functional characterization of bacteria isolated from different gut compartments of white grub, Anamola dimidiata, larvae“. Journal of Environmental Biology 41, Nr. 6 (15.11.2020): 1526–35. http://dx.doi.org/10.22438/jeb/41/6/mrn-1420.
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Aim: The aim of the present study was to isolate and characterize cellulolytic, lipolytic and nitrate reductase activities in the bacteria isolated from the gut of white grub, Anamola dimidiata larvae Methodology: Field collected third instar scarab larvae were dissected under aseptic conditions and inoculated on different bacteriological media to isolate gut bacteria. Identification of these isolates was carried out by amplifying and sequencing the 16S rRNA gene and comparing with their closest relatives in GenBank. Cellulolytic, lipolytic and nitrate reductase activities were assayed using Carbonmethyl cellulose (CMC), Rhodamine B and nitrate broth media. Results: The majority of culturable bacteria in the gut of A. dimidiata belonged to two phyla: Firmicutes (62.5%) and Proteobacteria (37.5%). Forty aerobic and eleven anaerobic bacterial strains were isolated and tested for cellulolytic, lipolytic and nitrate reductase activity, and twenty seven and thirty one cellulolytic and lipolytic gut bacteria were identified, respectively, with 19 isolates exhibiting both activities whereas ten facultative anaerobic bacteria isolates were positive for nitrate reductase activity. Interpretation: These bacterial isolates may be good sources for profiling novel isolates and enzymes for industrial use besides identifying new solutions for pest control.
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Astley, Roger A., Md Huzzatul Mursalin, Phillip S. Coburn, Erin T. Livingston, James W. Nightengale, Eddy Bagaruka, Jonathan J. Hunt und Michelle C. Callegan. „Ocular Bacterial Infections: A Ten-Year Survey and Review of Causative Organisms Based on the Oklahoma Experience“. Microorganisms 11, Nr. 7 (13.07.2023): 1802. http://dx.doi.org/10.3390/microorganisms11071802.
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Ocular infections can be medical emergencies that result in permanent visual impairment or blindness and loss of quality of life. Bacteria are a major cause of ocular infections. Effective treatment of ocular infections requires knowledge of which bacteria are the likely cause of the infection. This survey of ocular bacterial isolates and review of ocular pathogens is based on a survey of a collection of isolates banked over a ten-year span at the Dean McGee Eye Institute in Oklahoma. These findings illustrate the diversity of bacteria isolated from the eye, ranging from common species to rare and unique species. At all sampled sites, staphylococci were the predominant bacteria isolated. Pseudomonads were the most common Gram-negative bacterial isolate, except in vitreous, where Serratia was the most common Gram-negative bacterial isolate. Here, we discuss the range of ocular infections that these species have been documented to cause and treatment options for these infections. Although a highly diverse spectrum of species has been isolated from the eye, the majority of infections are caused by Gram-positive species, and in most infections, empiric treatments are effective.
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Talip Abd-alla, Mays, Mohsen Hashim Risan und Athraa H. Muhsin. „Microbial Contamination and Identification of Bacterial for Mobiles Phones in Iraq“. Al-Kufa University Journal for Biology 7, Nr. 2 (30.08.2015): 50–56. http://dx.doi.org/10.36320/ajb/v7.i2.8017.
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This study was conducted to isolate and identify bacteria contaminants on a mobile phone. The samples were collected randomly from 20 mobile phones. This study was conducted between October to December, 2016 at College of Biotechnology, Al-Nahrain University. The isolated colonies were then sub cultured in nutrient agar and slants in order to obtain pure culture of all the six colonies. Six genera of bacteria were identified from positive cultures. In all, 20 swab samples of mobile phone were randomly examined, 19 bacterial isolates were identified from mobile phones were found contaminated with microbiota. The highest prevalence of Staphylococcus aureus was observed in mobile phones. The research findings indicated that S. aureus (8 isolates), Escherichia coli (4 isolates), Enterobacter spp (2 isolates), Bacillus (1 isolates ), Streptococceus spp (1 isolates), and Pseudomonas spp (3 isolates), were the main isolates frequently associated with the mobile phones. Showed Percentage of bacterial isolates from the samples collected from mobile phones after calculating the total percentage of each isolate, found S. aureus, E. coli, Enterobacter spp, Bacillus, Streptococceus spp and Pseudomonas spp in the percentage of 42.10 %, 21.05 %, 10.52%, 5.26 %, 5.26 % and 15.78 % respectively. The results showed that mobile phones were contaminated with different types of bacteria mentioned above. Gram positive cocci, Streptococcus and Staphylococcus spp. were identified based on morphological characteristics. Gram negative bacilli, E. coli, Enterobacter, Bacillus and Pseudomonas were identified based on morphological characteristics. Nineteen isolates from 20 observed mobile phones belonging to the students. The highest prevalence in male was (13 isolates) and were percentage of bacteria isolated 66.66%, while in female were (6 isolates) and percentage of bacteria isolated 33.33%. Also showed results Percentage of total bacteria isolated of female and male, were 31.57% and 68.42 % respectively.
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Siangpro, Noppadon, Songkran Chuakrut, Wanna Sirimanapong, Somboon Tanasupawat, Wongsakorn Phongsopitanun, Bunyarit Meksiriporn, Jarungwit Boonnorat, Siripun Sarin, Siriwat Kucharoenphaibul und Rumpa Jutakanoke. „Lactiplantibacillus argentoratensis and Candida tropicalis Isolated from the Gastrointestinal Tract of Fish Exhibited Inhibitory Effects against Pathogenic Bacteria of Nile Tilapia“. Veterinary Sciences 10, Nr. 2 (07.02.2023): 129. http://dx.doi.org/10.3390/vetsci10020129.
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Nile tilapia is one of the most consumed farmed fish in the world. The outbreak of pathogenic bacterial diseases causes high mortality rates and economic losses in Nile tilapia farming. Antibiotic administrations are commonly utilized to inhibit and prevent bacterial infections. However, antibiotics are expensive and cause serious concerns for antibiotic resistance in fish that can be potentially transferred to humans. As an alternative solution, probiotics can be used to prevent infection of pathogenic bacteria in fish. In this work, both bacteria and yeast were isolated from fish gastrointestinal tracts and their inhibitory activity against Nile tilapia pathogenic bacteria was evaluated, as well as other probiotic properties. In this study, 66 bacteria and 176 acid tolerant yeasts were isolated from fish gastrointestinal tracts. Of all isolated microorganisms, 39 bacterial and 15 yeast isolates with inhibitory effect against pathogens were then examined for their probiotic properties (acidic and bile salt resistance, adhesion potential, and biofilm formation), formation of antibacterial factor survival rate under simulated gastrointestinal fluid, and safety evaluation. AT8/5 bacterial isolate demonstrated probiotic properties and the highest inhibition against all 54 tested pathogens while YON3/2 yeast isolate outperformed the inhibitory effect among all yeast isolates. These two probiotic isolates were further identified by 16S rDNA and the D1/D2 domain of 26S rDNA sequence analysis for bacterial and yeast identification, respectively. AT8/5 and YON3/2 showed the highest similarity to Lactiplantibacillus argentoratensis and Candida tropicalis, respectively. This is the first report on isolated L. argentoratensis and C. tropicalis with antipathogenic bacteria of Nile tilapia properties. Collectively, AT8/5 and YON3/2 could be potentially used as promising alternatives to existing antibiotic methods to prevent pathogenic bacteria infection in Nile tilapia farming.
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Fajri, Muhammad Danial, Subehan Lallo und Sartini Sartini. „PRELIMINARY STUDIES ON ISOLATION AND SCREENING OF ANTIBIOTIC PRODUCING SYMBIOTIC BACTERIA FROM STARFISH (Protoreaster nodosus) COLLECTED FROM COASTAL AREA TAKALAR REGENCY, INDONESIA“. Journal of Experimental Biology and Agricultural Sciences 9, Nr. 2 (25.04.2021): 183–88. http://dx.doi.org/10.18006/2021.9(2).183.188.
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Marine organisms are well known for the availability of bioactive compounds which have various biological activities including antibacterial activity. Likewise, their symbiotic bacteria can also produce compounds that have similar activities. The purpose of this study was to isolate and screen the symbiotic bacteria from starfish (Protoreaster nodosus) collected from coastal area Takalar Regency, South Sulawesi, Indonesia. Isolation was carried out by the pour plate method using nutrient agar medium dissolved in sterile seawater. The isolated symbiotic bacteria were purified by using the quadrant method. The pure isolate was culture through submerged fermentation using nutrient broth media enriched with 1% yeast extract and sterile seawater for 7 days. The selected symbiont bacterial isolates were tested for their antibacterial activity against gram-positive and gram-negative bacteria using disc diffusion assays. The results of fermentation were separated from the biomasses and tested for antibacterial activity against Staphylococcus aureus (S. aureus, ATCC 25923), Bacillus subtilis (B. subtilis, ATCC 6633), Salmonella typhi (S. typhi, NCTC 786), and Escherichia coli (E. coli, ATCC 25923). The results of study revealed that four symbiotic bacteria (SB 1T, SB 2T, SB 3T, and SB 4T) were successfully isolated. All the SB isolates have good antibacterial activity against all tested bacterial strains with an average diameter of inhibition zone larger than 11 mm. Among all isolates, isolate SB 4T showed a remarkable size of zones growth inhibition (> 15 mm) against all tested bacterial strains. Thus, the symbiotic bacteria isolated from P. nodosus in this study have a promising broad-spectrum antibacterial activity.
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Qays, Hawra, Asia Fadhile Almansoory und Israa Abdulwahab Al-Baldawi. „Interaction Between Typha domingensis and Bacteria Bacillus sp. to Treatment of Wastewater Polluted by Kerosene“. IOP Conference Series: Earth and Environmental Science 1215, Nr. 1 (01.07.2023): 012047. http://dx.doi.org/10.1088/1755-1315/1215/1/012047.
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Abstract Water pollution with Kerosene is considered as a serious environmental problem in Basrah. Typha domingensis was used to treat kerosene-contaminated water in the construction wetland system. The Isolated bacteria was the most efficient in degradation Kerosene. Bacteria isolated from contaminated water by hydrocarbons of four stations in Basrah, these isolates were tested to show efficiency in degrading hydrocarbons to be used in phytoremediation of water contamination with hydrocarbons. Twelve bacterial isolates were identified as Bacillus sp. The Isolated bacteria (B11) was the most efficient in degradation oil hydrocarbons based on these used in the phytotoxicity test. The phytotoxicity experiment was conducted for 72 days in two glass basins containing water contaminated with 5% kerosene concentration with T. domingensis. The Isolate bacterial were added to two glass basins to test the interaction of the bacterial degradation with the plant and two contaminated ponds without plant and two with pollutant and bacteria without plant with control basin free from pollutant, the temperature between (20.6-36.2)°C, dissolved oxygen between (2.5-7.4) mg / L. The rate of kerosene removal from the water contaminated in the ponds of the plant without bacteria and plant with bacteria, bacteria only and control (76.5%), (81.9)%, (74.1%), (57.4) respectively.
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Sedijani, Prapti, Bq Novi Aprilia, Dewa Ayu Citra Rasmi und Kusmiyati Kusmiyati. „Isolation and Screening of Amilolytic Bacteria Isolate from Cassava (Manihot utilissima)“. Jurnal Biologi Tropis 23, Nr. 4 (05.09.2023): 226–32. http://dx.doi.org/10.29303/jbt.v23i4.5604.
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Amylase is an enzyme capable of hydrolyzing starch into simpler sugars. The use of amylase enzymes is urgently needed in various industrial fields, therefore the search for sources of amylase enzyme producers continues to be carried out for sources of amylase enzyme producers. Each industrial field has specific requirements for amylase enzymes. This study aims to isolate bacteria capable of producing amylase enzyme and to determine the amylase activity of bacteria isolated from cassava (Manihot utilissima) when incubated at different temperatures (room temperature and 30℃) and pH levels (7, 8, 9, and 10). The research involved bacterial isolation, testing the amylase activity of the isolated amylolytic bacteria at varying pH and temperatures, and characterizing the amylolytic bacteria. The amylolytic bacterial activity was qualitatively measured based on the clear zone's area formed around the colony. From this study 5 isolates showing amylolytic activity were obtained. The amylolytic activity index varied among the isolates, ranging from 0.12 to 0.59. The highest amylolytic activity index (0.59) was performed by S4 isolate on pH 10 medium incubated at 30℃. Microscopic and gram staining analysis suggest that two isolates were gram-negative coccus bacteria and three of them were gram-positive coccobaccilus bacteria.
Dissertationen zum Thema "Bacteria isolated"
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Willard, Kyle. „Investigation of exopolysaccharide producing bacteria isolated“. Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71627.
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Thesis (MSc)--Stellenbosch University, 2012.ENGLISH ABSTRACT: The deterioration of harvested sugarcane as a result of bacterial growth causes major losses of sucrose and a build-up of exopolysaccharides (EPS). Polysaccharides present during production increase the massecuite viscosity, which negatively influences evaporation and crystallisation. In this study 38 culturable EPSproducing bacteria were isolated from milled sugarcane. Analysis of the EPS showed the ubiquitous presence of glucose, however, 14 polysaccharides also contained mannose, fructose or galactose. In vitro treatment using Chaetomium erraticum dextranase to evaluate is effectiveness indicated that 37 of the EPS were hydrolysed to some extent. There were 21 polysaccharides that were only partially digested. The capacity of the isolates to produce EPS on different sugars indicated a correlation between sucrose and polysaccharide formation in 37 isolates. The results indicate there are more species involved in EPS production than previously thought as well as the presence of non-dextran polysaccharides.
AFRIKAANSE OPSOMMING: Bakteriële groei veroorsaak ‘n afname in gehalte, sukrose en ‘n verhoging in die hoeveelheid van eksternepolisakkeriede (EPS). Die verhoogde konsentrasie van polysakkariede gedurende die verwerkingsprosses veroorsaak ‘n verhoging in “massecuite” viskositeit. Hierdie verskynsel het ‘n nadelige uitwerking op die verdamping en kristalvorming van die produk. In gemaalde skuikerriet was 38 groeibare EPS-produserende bakterieë geisoleer. Die geanaliseerde EPS van hierdie bogenoemde bakterieë was daar in almal glukose teenwoordig. In 14 van hulle was mannose, fruktose en galaktose ook gevind. Die in vitro effektiwieteit van Chaetomium erraticum dekstranase op die EPS het gewys dat 37 het tot ‘n mate gehidroliseer maar 21 was net gedeeltelik verteer. As gevolg van die bo-genoemde resultate was daar gevind dat sukrose was ‘n noodsaaklike subtraat vir EPS produksie in die geisoleerde bakterieë. In hierdie studie was bevestig ‘n groter verskiedenheid EPS-produserende bakterieë gevind was en dat hulle assosiasie aan sukierriet prossering meer kompleks is as wat vooreen gedink was.
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Sislak, Christine Demko. „Novel Thermophilic Bacteria Isolated from Marine Hydrothermal Vents“. PDXScholar, 2013. https://pdxscholar.library.pdx.edu/open_access_etds/1486.
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As part of a large study aimed at searching for patterns of diversity in the genus Persephonella along the north to south geochemical gradient of the ELSC, ten novel strains of Alphaproteobacteria were isolated unexpectedly. Using defined media under microaerophilic conditions to enrich for Persephonella from chimney samples collected at the seven vent fields on the ELSC and the dilution to extinction by serial dilution method to purify cultures, a total of ten strains belonging to the Alphaproteobacteria were isolated. Two of these isolates, designate MN-5 and TC-2 were chosen for further characterization and are proposed as two new species of a novel genus to be namedThermopetrobacter. Both strains are aerobic, capable of chemoautotrophic growth on hydrogen and grow best at 55°C, pH 6 and 3.0% NaCl. Strain MN-5 is capable of heterotrophic growth on pyruvate and malate and TC-2 is only able to grow heterotrophically with pyruvate. The GC content of MN-5 is 69.1 and TC-2 is 67 mol%. GenBank BLAST results from the 16S rRNA gene reveal the most closely related sequence to MN-5 is 90% similar and the most closely related sequence to strain TC-2 is 89% similar.
Sampling at a shallow marine vent on the coast of Vulcano Island, Italy in 2007 led to the isolation of a novel species of Hydrogenothermus, a genus within the Hydrogenothermaceae family. This isolate, designated NV1, represents the secondHydrogenothermusisolated from a shallow marine vent. NV1 cells are rod-shaped, approximately 1.5μm long and 0.7μm wide, motile by means of a polar flagellum and grow singularly or in short chains. Cells grow chemoautotrophically using hydrogen or thiosulfate as electron donors and oxygen as the sole electron acceptor. Growth was observed between 45 and 75°C with an optimum of 65°C (doubling time 140 min), pH 4.0-6.5 and requires NaCl (0.5-6.0% w/v). The G+C content of total DNA is 32 mol%.
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Marais, Laurette Marlize. „Characterization of bacteria isolated from a platinum mine tailings dam / Laurette Marais“. Thesis, North-West University, 2012. http://hdl.handle.net/10394/8721.
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Contamination from various sources has a huge impact on soil health and microbial community composition. Metal contamination of soil in mining scenarios is of concern and is not adequately addressed, particularly with respect to the microbial community. The mining industry is one of the largest contributors to heavy metal contamination of soil in South Africa, especially since the country is one of the major mining countries in the world. Platinum mining is of special importance, since the largest percentage of the world’s reserves of platinum group metals are found and mined in South Africa. Metals from mining activities become irreversibly immobilized in soil systems because they cannot be degraded and has a huge impact on soil systems. In this study, bacteria was isolated from soil samples collected from a platinum mine tailings dam outside Rustenburg. During the warm sampling season (March 2006) most isolates were found, especially in sites 3 and 4. During the colder and drier season (May 2006) there were less isolates. Most of the isolated cultures also displayed a wide temperature growth range, mostly between 24°C - 37°C. Paenibacillus lautus and Bacillus subtilus DN-10 had a growth range between 5°C - 40°C. Culturable metal tolerant bacteria were isolated, purified and identified using 16S rDNA sequences. Nine different species were found namely Paenibacillus lautus strain DS19, Paenibacillus lautus, Paenibacillus sp. C15, uncultured Paenibacillaceae, Bacillus subtilis strain DN-10, Bacillus sp. KDNB5, Bacillus cereus, Stenotrophomonas maltophilia and Alcaligenes sp. DJWH 146-2. The ability of these strains to tolerate metal concentrations were explored by determining their minimum inhibitory concentrations for a selection of metals e.g. aluminum, barium, cobalt, chromium, cadmium, copper, iron, lead, manganese, nickel and mercury. Most isolates were able to tolerate >5mM of the Al\Ni alloy and cobalt. Transmission electron microscopy was used to determine the location of metals inside bacterial cells and electron dispersive X-ray analysis was used to determine the levels of metals inside microbial cells. Bacillus subtilis DN-10 (LDK0306) showed a high MIC (>5mM) for most metals used, except Hg. This strain also had a high percentage (10.26%) of Pb detected in its cells by EDX. This was the highest percentage detected. Plasmids were extracted from the identified strains and can help gain a better understanding of metal tolerance mechanisms used by these isolates.Thesis(MSc (Environmental Sciences))--North-West University, Potchefstroom Campus, 2013
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Abou, Assaf Nasser. „Degradation of the herbicide EPTC by isolated soil bacteria /“. The Ohio State University, 1991. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487693923199045.
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Park, Chan-Woo. „Effective organic acid fermentation of garbage by isolated bacteria“. 京都大学 (Kyoto University), 2004. http://hdl.handle.net/2433/145366.
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Webb, Martin Darren. „Biotransformation of pentachlorophenol by actinomycetes isolated from compost“. Thesis, University of Liverpool, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243205.
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Nandivada, Lakshmi Sarada. „Beta-lactam resistance in gram-negative bacteria isolated in India“. Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/27101.
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The most important resistance mechanism to beta-lactam antibiotics is the plasmid-mediated beta-lactamase and the common criterion for the epidemiology of these enzymes is the determination of their biochemical characteristics. Surveys of plasmid-encoded beta-lactamases of Gram-negative bacteria, used to investigate their relative clinical importance, have been poorly performed and rarely conducted outside the developed world. A survey of uropathogenic strains and of salmonellae and shigellae, isolated in south India in 1984, revealed a higher incidence of ampicillin resistance (minimum inhibitory concentration [MIC] > 10mg/1) than had ever been reported before (Enterobacteriacea 82%, salmonellae 90%, shigellae 60%). Only the enterobacterial strains showed any significant resistance to the first generation cephalosporin, cephalosporin, cephaloridine (MIC > 10mg/1). However, 66% of the salmonellae strains were cefuroxime resistant. A small proportion of all species conferred resistance to third generation cephalosporins. In the individual species, there was a very high incidence of ampicillin resistance (E. coli 76% Klebsiella spp 96%) and cephaloridine resistance (E.coli 57% Klebsiella spp 69%). Many of the ampicillin resistant strains harboured either auto-transferable or mobilisable plasmids (40%). Characterisation of the plasmid DNA from the E.coli transconjugants revealed the existence of 37 different plasmids types. The transconjugants from klebsiella, salmonella and shigella possessed fewer plasmids types than those from E. coli. Most plasmids possessed resistance genes to aminoglycosides and to six or more drugs. Beta-lactamase studies revealed that TEM-1 was the most predominant enzyme in all transconjugant strains followed by OXA-1, SHV-1, TEM-2, OXA-2 and the novel enzyme SAR-2. The SAR-2 enzyme was fully characterised and had a higher pI (8.3) than any previously characterised plasmid-mediated beta-lactamase. It had a broad-spectrum activity with the molecular weight of 36000. In addition the unusual observations of E.coli strains producing both the PSE-1 and PSE-2 beta-lactamases and strains hyperproducing the TEM-1 were made and these strains were studied further. The development and mechanisms of resistance to beta-lactam/beta-lactamase inhibitor combinations (ampicillin and clavulanic acid) have been performed with laboratory strains possessing the ampicillin resistance plasmid R1. The results show that challenge with clavulanic acid alone did not affect the expression or integrity of the beta-lactamase whereas challenge with the combination of ampicillin and clavulanic acid caused radical changes with the expression of the beta-lactamase. In some cases there were multiple copies of genes which resulted in hyperproduction of TEM-1 enzyme and this was sufficient to resist the combinations. Unfortunately, these variants also conferred resistance to second and third generation cephalosporins. Evidence of this type of resistance to clavulanic acid is now emerging in clinical practice.
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Xiraphi, Polyhronia. „Safety attributes of lactic acid bacteria isolated from fermented sausages“. Thesis, University of Surrey, 2009. http://epubs.surrey.ac.uk/843262/.
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Lactic acid bacteria (LAB) were isolated during the production and the ripening of Greek dry fermented sausages. Samples were taken at different stages, and 150 "wild' strains were isolated. The majority of the strains isolated were assigned to the species Lactobacillus plantarum biotype (1) (43.3 %) followed by Lb. curvatus, Lb. pentosus (10.7 %), Lb. brevis biotype (1) (8.7 %), Lactococcus lactis subsp. lactis biotype (1) (6.7 %) and Leuconostoc mesenteroides subsp. mesenteroid.es biotype (2) (5.3 %). The possibility of bacteriocin production was tested using the agar well diffusion assay (AWDA). One strain was found to produce bacteriocin (Leuconostoc mesenteroides E131) and its purification was attempted using precipitation with ammonium sulphate, cation exchange, and reverse phase chromatography. Moreover, the purification of curvaticin L442 was attemped, a bacteriocin produced by Lactobacillus curvatus L442, isolated from Greek traditional fermented sausage prepared without addition of starters. The bacteriocin was purified by 50% ammonium sulphate precipitation, cation exchange, reverse phase and gel filtration chromatography. Lb sakei I154, a bacteriocin producing strain isolated from Italian fermented sausage, and the semi-purified bacteriocin from Leuconostoc mesenteroides E131 were validated via industrial trials to evaluate whether the product (fermented sausages) maintains the technological characteristics and the traditional quality characteristics. Three fermentations under controlled conditions were conducted and at the end of these fermentations, products were sliced and packaged under controlled atmosphere (80% N2 + 20% CO2) and stored at 4+/-2 °C for 12 weeks to determine the shelf life of the product. Finally, from the previous industrial trials the proper production parameters were determined as well as the most effective packaging techniques, resulting in the conduction of a Standard Operation Procedures Guide concerning the whole production of traditional fermented sausages.
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Gerard, Jeffery M. „Antibiotic secondary metabolites of bacteria isolated from the marine environment“. Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25055.pdf.
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Ramirez-Lopez, Lina Marcela. „Heat inactivation of thermo-resistant bacteria isolated from poultry offal“. Connect to this title online, 2006. http://etd.lib.clemson.edu/documents/1171902361/.
Der volle Inhalt der QuelleBücher zum Thema "Bacteria isolated"
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Gravelle, Louise N. The antibiotic resistance of bacteria isolated from dental unit waterlines. Sudbury, Ont: Laurentian University, School of Graduate Studies, 2005.
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Ward, T. Interactions of selected bacterial isolates with DBT and solubilized coal. S.l: s.n, 1990.
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Luckarift, Heather Rosemary. The production of chiral hydroxylated products from new bacterial isolates. [s.l.]: typescript, 1998.
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Deppe, Uta. Degradation of crude oil at low temperatures by a newly isolated psychrotolerant bacterial consortium. Berlin: Mensch & Buch Verlag, 2003.
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Watkins, Peter Gareth. The corrosion of mild steel in the presence of two isolates of marine sulphate reducing bacteria. Portsmouth: University of Portsmouth, School of Pharmaceutical [sic.] and Biomedical Sciences, 1998.
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Davis, Robert Patrick Matthews. Effect of nutrient variation on the growth of a bacterial species isolated from an activated sludge plant. [S.l: The Author], 1985.
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Nazarali, Zaida. The effect of acidophilic bacteria on the leaching of low-grade Cu-Ni ore by different isolates of Acidithiobacillus ferrooxidans. Sudbury, Ont: Laurentian University, School of Graduate Studies, 2004.
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Watthanachōt, Čhančharat, Hrsg. Kānsưksā prōtīn thī mī rit tān čhulinsī čhāk bǣkthīrīa thī yǣk dai čhāk fō̜ngnām thalē =: A study of antimicrobial proteins of marine bacterial isolate from some sponges : rāingān kānwičhai chabap sombūn. [Chonburi]: Sathāban Witthayāsāt thāng Thalē, Mahāwitthayālai Būraphā, 2006.
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Varieties of colon bacilli isolated from man. [S.l: s.n., 1986.
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Lopes, Carlos Alberto. Biological control of Pseudomonas avenae with epiphytic bacteria isolated from corn plants. 1986.
Den vollen Inhalt der Quelle findenBuchteile zum Thema "Bacteria isolated"
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Patil, Mahalakshmi S., Raghu Ram Achar und Ann Catherine Archer. „Characterization of Probiotic Properties of Isolated Bacteria“. In Springer Protocols Handbooks, 105–17. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3032-7_15.
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Wang, S., und A. Kelman. „Characteristics of Strains of Pseudomonas Fluorescens Isolated from Soil in Relation to Ability to Macerate Plant Tissue“. In Plant Pathogenic Bacteria, 277–82. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_58.
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Nes, Ingolf F., Christina I. Mørtvedt, Jon Nissen-Meyer und Morten Skaugen. „Lactocin S, A Lanthionine-Containing Bacteriocin Isolated from Lactobacillus Sake L45“. In Bacteriocins of Lactic Acid Bacteria, 435–49. Boston, MA: Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2668-1_17.
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Saha, Mousumi, Agniswar Sarkar und Bidyut Bandyopadhyay. „Phylogenetic Characterization of Nitrifying Bacteria Isolated from East Kolkata Wetland“. In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022), 114–22. Dordrecht: Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_12.
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AbstractEast Kolkata Wetland (EKW) is an “International Ramsar Site”, famous for broad biodiversity and insightful use of sewage for aquaculture. Native nitrifying bacteria of EKW play a significant role in maintaining water quality and controlling environmental pollution by converting ammonia into nitrate in wastewater. Therefore, the characterization of nitrifying bacteria is important in EKW. Thus, the main focus of this research was to identify and characterize the nitrifying bacteria, investigating their phylogeny and diversity in EKW. 16S rRNA and functional genes analysis may help in the proper evaluation of composition and distribution of nitrifying bacteria in some water bodies in EKW, which has not yet been explored. Molecular and phylogenetic characterization was targeted and achieved through 16S rRNA and functional gene analysis, followed by computational estimation. Resulted sequences were analysed to gain insight into the knowledge for global and local taxonomic orientation. Hence, a model can be created for characterizing the dynamics of nitrifying bacteria in wastewater treatment and sustainable aquaculture in different water bodies of EKW. Graphical Abstract
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Asker, Dalal, Tarek S. Awad, Teruhiko Beppu und Kenji Ueda. „Novel Zeaxanthin-Producing Bacteria Isolated from a Radioactive Hot Spring Water“. In Microbial Carotenoids from Bacteria and Microalgae, 99–131. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-879-5_5.
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Blanc, Michel, Trello Beffa und Michel Aragno. „Biodiversity of Thermophilic Bacteria Isolated from Hot Compost piles“. In The Science of Composting, 1087–90. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-1569-5_113.
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Lim, Seul Ki, und Hak-Jong Choi. „Health Benefits of Lactic Acid Bacteria Isolated from Kimchi“. In ACS Symposium Series, 107–19. Washington, DC: American Chemical Society, 2019. http://dx.doi.org/10.1021/bk-2019-1303.ch008.
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Drewniak, Lukasz, Aleksandra Styczek und Aleksandra Sklodowska. „Arsenic Hypertolerant Bacteria Isolated from Gold Mine Rocks Biofilms“. In Advanced Materials Research, 576. Stafa: Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/0-87849-452-9.576.
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Prana, T. K., M. Abe, T. Uchiumi, M. S. Prana, T. Seki, K. Fujiyama und S. Higashi. „Characterization of Root Nodule Bacteria Isolated from Paraserianthes falcataria“. In Biological Nitrogen Fixation for the 21st Century, 520. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5159-7_330.
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Sarkar, Sutripta, und Anubrati Chatterji. „Characterization of Lipase-Producing Bacteria Isolated from Degrading Oil Cakes“. In Utilization and Management of Bioresources, 253–60. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5349-8_24.
Der volle Inhalt der QuelleKonferenzberichte zum Thema "Bacteria isolated"
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Kumalasari, Yeni Indra, Agung Dian Kharisma und Sri Yuwantiningsih. „Potential of Karimunjawa Island’s Plants as Antibiotic-Producing Endophytic Bacteria Sources“. In The 2nd International Conference on Technology for Sustainable Development. Switzerland: Trans Tech Publications Ltd, 2022. http://dx.doi.org/10.4028/p-kv25ou.
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Endophytic bacteria have a great potential to be applied as biofertilizers and biopesticides, but their information as a source of antibiotics still needs to be developed and explored. The aim of this study was to investigate the potential sources of antibiotics in endophytic bacteria isolated from the stems of Setigi, Wahong, Bongko, Kalimosodo, Dewandaru, and Legundi plants on Karimunjawa Island. Molecular approaches were performed to isolate, characterize, and identify bacterial endophytes as potential antibiotic sources by plate assay and 16S rRNA gene sequence analysis. Dewandaru isolate was identified as gram-negative bacteria, whereas; gram-positive bacteria were detected in other isolates. Moreover, Setigi and Dewandaru isolates showed the highest level to inhibit the growth of Fusarium sp and displayed 99% similarity with antibiotic-producing bacteria, namely Bacillus pumilus and Bacillus cereus, respectively. These results indicate the possibility of antibiotic activities by Setigi and Dewandaru isolated. Therefore, it is assumed that both Setigi and Dewandaru isolates potentially appeared as new antibiotics sources from local plants. This study provides novel insight into the future production of novel antibiotics derived from plant-associated endophytic bacterial as a strategy for increasing the application of natural compounds to control plant diseases in agriculture.
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Samoilova, Anna. „Effect of phages isolated from different sources against fire blight pathogen“. In 5th International Scientific Conference on Microbial Biotechnology. Institute of Microbiology and Biotechnology, Republic of Moldova, 2022. http://dx.doi.org/10.52757/imb22.29.
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Fire blight of rosaceous plants is one of the economically most important diseases of fruit trees caused by the bacterium Erwinia amylovora. Plants are extremely vulnerable for fire blight infection at the bloom stage. Blossom blight can lead to the great crop losses and even the plant death. Since chemical treatments are forbidden in time of blossoming, bacteriophages, highly specific bacterial viruses could be used for the disease control. Being the natural components of ecosystems, phages infect only bacteria sensitive to them, are non-toxic to plants, animals and humans and are adapted to the bacteria environment. It has been shown that bacterium E. amylovora expresses its major pathogenicity factors during immature pear tissues infection. Therefore, in this study, the ability of four virulent E. amylovora bacteriophages, isolated from the aerial parts of the affected plants (phage isolate 1 from quince tissues; phage isolate 2 from hawthorn, Republic of Moldova) and from natural water reservoirs near fruits orchards or wild rosaceous trees (phage isolates 3 and 4, Swiss Confederation) to inhibit E. amylovora growth in the immature pear tissues was evaluated. Immature pear slices were inoculated with suspensions of E. amylovora CFBP1430 and EaM contained 104 CFU/ml. After four hours incubation in the humidified chamber at 280C infected immature pear slices were treated with 107 PFU/ml of phage isolates. Pear slices, treated with sterile distilled water were used as a control. Symptoms were recorded at 1, 2, 3, 5, 6, 7 and 8 days after inoculation. For each bacteria strain/phage isolate combination tested pear slices were assayed in triplicate and each experiment was repeated at least two times. Immature pear slices infected with bacteria EaM displayed the first symptoms of the fire blight, ooze formation and light necrosis, one day after inoculation. Pear slices, infected with E. amylovora CFBP1430 demonstrated ooze and necrosis two days after inoculation. In the bacteria/phage combinations the first symptoms of the fire blight appeared on the sixth day after inoculation in the variants of EaM/phage isolate 3 and CFBP1430/phage isolate 3. On the seventh and eighth days after inoculation symptoms of the fire blight infection have been recorded in the EaM/phage isolate 2 and CFBP1430/ phage isolate 2, respectively. Bacteria/phage combinations EaM/phage isolate 4 and CFBP1430/ phage isolate 4 showed disease symptoms on the seventh day after inoculation. Immature pear slices in the variants EaM/phage isolate 1 and CFBP1430/phage isolate 1 showed necrotic lesion eight days after inoculation. Thus, phage isolate 4, detected in water was able to suppress growth of phytopathogenic E. amylovora just a day less than highly virulent phage isolate 1 detected in the quince tissues. The conducted experiments have demonstrated that bacteriophages isolated from water revealed high efficacy against bacteria E. amylovora and all studied phage isolates successfully inhibited the fire blight causative agent growth in the plant host tissues for about seven days. Hence it has been shown that treatment with bacteriophages for the fire blight control in the fruit orchard should be carried out weekly if environmental conditions are favorable for the disease development.
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Siripornadulsil, Surasak, und Wilailak Siripornadulsil. „Characterization of Cadmium-Resistant Bacteria and Their Application for Cadmium Bioremediation“. In ASME 2009 12th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2009. http://dx.doi.org/10.1115/icem2009-16072.
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On a global basis, trace-metal pollution is one of the most pervasive environmental problems. It is particularly difficult to prevent or clean up because the metals are toxic in their elemental form and cannot be decomposed. Bioremediation has been shown to be a powerful system for heavy metal pollution clean up and prevention. In this work, we characterized the cadmium (Cd)-resistant bacteria isolated from rice field soil downstream from zinc (Zn) mineralized area which the owners were contaminated at high level of cadmium content in their blood (>10 μgCd/g creatinine). We found that all 24 isolated bacteria tolerated toxic Cd concentrations (2,500 μM). In order to determine whether the Cd toxicity affected the growth of isolated bacteria, we grew the isolated bacterial cells in the absence and presence of toxic concentrations of CdCl2 (500 μM). In the absence of Cd, all isolated bacterial cells grew slightly better than in the presence of toxic concentrations of Cd. In addition, the Cd binding capacity of all isolated bacteria were very high, ranging from 6.38 to 9.38 log[Cd(atom)]/cell when grown in the presence of 500 μM CdCl2. Furthermore, the stability of Cd-bacteria complex of all isolated bacteria was affected by 1mM EDTA. When grown in the presence of 500 μM CdCl2, Cd-resistant isolates S2500-6, -8, -9, -15, -17, -18, -19, and -22 increasingly produced proteins containing cysteine (SH-group) (from 1.3 to 2.2 times) as well as 11 isolates of Cd-resistant bacteria, including S2500-1, -2, -3, -5, -6, -8, -9, -11, -16, -20, and -21, increasingly produced inorganic sulfide (1.5 to 4.7 times). Furthermore, the Sulfur K-edge X-ray absorption near-edge structure (XANES) spectroscopy studies indicated that Cd-resistant isolated S2500-3 precipitated amounts of cadmium sulfide (CdS), when grown in the presence of 500 μM CdCl2. The results suggested that these Cd-resistant bacteria have potential ability to precipitate a toxic soluble CdCl2 as nontoxic insoluble CdS. Interestingly, Cd-resistant bacteria isolated S2500-3, -8, -9,and -20 increased cadmium tolerance of Thai jasmine rice (Kao Hom Mali 105) when grown in the presence of 200 μM CdCl2. These 4 isolates also decreased cadmium concentration accumulation in Kao Hom Mali 105 plant at 61, 9, 6, and 17%, respectively when grown in the presence of 200 μM CdCl2. They were identified by 16S rDNA sequence analysis and classified as Cupriavidus taiwanensis (isolate S2500-3) and Pseudomonas aeruginosa (isolates S2500-8, -9, and -20).
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Rammadan ABDUL, Fatima, Maysoon Kh.ABBAS, Batool Abd Al Ameer BAQER und Ihsan Ali RAHEEM. „ASSESSMENT OF THE LEVEL OF INTERLEUKIN- 10 AND INTERFERON -GAMMA IN CHILDREN INFECTED WITH AEROMONAS HEMOPHILIA ISOLATED FROM DIARRHEA“. In VIII.International ScientificCongressofPure,AppliedandTechnological Sciences. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress8-4.
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Background: Aeromonas hydrophila causes bacterial diseases that lead to intestinal and extra-intestinal infections. Objective: The study aimed to isolate Aeromonas hydrophila from patients suffering diarrhea and evaluate the level of some interleukins in patients and compare them with control and resistance of bacteria to antibiotics. Materials and methods collect 150 stool samples of patients with diarrhea. Six isolates of A. hemophilia were isolated from children suffering diarrhea from some Baghdad hospitals. Results, all bacterial isolates were identified by the biochemical, cultural and microbial characteristics and confirmed by VITK2 System. The study showed the resistance of A. hemophilia to many antibiotics, the highest resistance to Tetracycline and Oxacillin and the rate of resistance was 100%, while the bacteria had the lowest resistance 16.7%, to to Erythromycin. The present results exhibited Interleukin-10 level was elevated in the patients and it was controlled by a significant level. The present results besides exhibited that the concentration of Interferon- gamma (IFN-γ) of patients and control group with nonsignificant difference
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Sultana, Sharmin, Md Sad Salabi Sawrav, Snygdha Rani Das, Mehfuz Alam, Md Abdul Aziz, Md Al-Amin Hossain und Md Azizul Haque. „Isolation and Biochemical Characterization of Cellulase Producing Goat Rumen Bacteria“. In International Conference on Emerging Trends in Engineering and Advanced Science. AIJR Publisher, 2022. http://dx.doi.org/10.21467/proceedings.123.12.
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Cellulose is the most prevalent polymer on the planet and has long been utilized for a variety of industrial applications. The study's goal was to screen and isolate cellulase-producing bacteria from the rumen of a goat collected from different location of Dinajpur district. To do so, rumen content samples from two distinct goats were collected. In this investigation, rumen cellulase-producing bacteria were isolated and characterized after serial dilution of five isolates up to six fold and inoculation into Nutrient agar. Following that, all of the isolates were underwent Methyl Red (MR) test & Voges-Proskauer (VP) test to identify organism’s metabolic pathway, Triple Sugar Iron Agar (TSI) Test to determine bacterial ability to utilize sugar, Motility Indole and Urease activity test (MIU) to determine motility, Urease utilization and can produce Indole or not, Citrate utilization test to utilize citrate as carbon and energy source, Oxidase test, Catalase test to check the presence of catalytic enzyme. The result revealed the colonial characterization of bacteria and also where proven all five isolates are promising enough and superior in quality to produce cellulose.
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Fouad Ali, Layla. „Antimicrobial Effect of Gaultheria Procumbens On Multidrug Resistant Bacteria Causing Wound Infections“. In IX. International Scientific Congress of Pure, Applied and Technological Sciences. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress9-27.
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Worldwide, a fast rise of resistant bacteria threatens the effectiveness of antibiotics, so alternatives should be used to overcome this serious problem. In this study a plant extract was used against multidrug resistant bacteria. Eight Klebsiella pneumonia and eight Staphylococcus aureus isolates were obtained from wounds infections. They were isolated and identified by biochemical tests and confirmed by using Vitek-2 test. The plant Gaultheria procumbens was extracted by. Susceptibility test was performed for nine antibiotics against all 16 isolated bacteria, this study brings to light that Klebsiella pneumonia isolates were 100% resistant to most antibiotics but they were sensitive with 12.5%, 75% and 75% to clindamycin, imipenem and meropenem respectively, while Staphylococcus aureus isolates were sensitive with 25% to imipenem and meropenem only. Minimal Inhibitory Concentration was assessed by using microtiter plate for plant extract and meropenem to all isolates. The plant extract subMICs were used against biofilm formation in Klebsiella pneumonia and Staphylococcus aureus isolates. Biofilm inhibition was calculated for each isolate, furthermore, biofilm formation was reduced in most Staphylococcus aureus isolates more than in Klebsiella pneumonia isolates
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Hewagama, H. L., G. M. T. K. Somarathna, L. Herath und S. E. Peiris. „Living Colours: Development of Microbial Culture Collection for Use as Microbial Colour Pigments in Textile Dyes“. In SLIIT International Conference on Advancements in Sciences and Humanities 2023. Faculty of Humanities and Sciences, SLIIT, 2023. http://dx.doi.org/10.54389/ccoj7801.
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The textile industry is one of the largest worldwide polluters of clean water due to the heavy use of synthetic dyes. Synthetic dyes are harmful to aquatic life and to human health. To overcome this, natural dyes are being explored as a healthier and more eco-friendly alternative. Several advantages such as ease of extraction, availability, high yields and no seasonal variation make microbial pigments the most ideal source of natural pigments. This study was done to isolate colour pigment producing bacteria and fungi from soil collected from organic farms from various locations in Sri Lanka. In total, 9 pigment producing bacteria and 3 pigment producing fungi were isolated. Gause’s synthetic agar yielded the most pigmented isolates. Extracellular pigments produced by 5 of the bacterial isolates were extracted by a water based method. The antibacterial activity of the pigments in their crude and concentrated forms was tested using the well diffusion method against E.coli ATCC 8739 and Staphylococcus aureus ATCC 6538P. Inhibition zone against S.aureus was observed for both crude (12.33±0.58mm) and concentrated pigments (9.67±0.58mm) extracted from purple pigment producing bacterial isolate (BPU). This pigment has the potential to be used in antibacterial textile preparation. Extracted pigments were used to dye scoured cotton fabric with the use of 3% alum as mordant. Pigment from BPU isolate resulted in better coloured fabric.
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Khair, Nedaa Kamalalden. „Activity of Antibiotic Producing Bacteria Isolated from Rhizosphere Soil Region of Different Medicinal Plants“. In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0093.
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The rhizosphere soil of medicinal plants is rich in microorganisms that develop antibiotics as natural mechanism of protection against other microbes that live in their vicinity. The present study aims to explore the production of antibacterial agents from rhizosphere soil bacteria of 11 medicinal plants and determine their activity against Gram-negative (Pseudomonas aeruginosa, Escherichia coli) and Gram-positive (Bacillus cereus, Staphylococcus aureus) bacteria. Soil samples were collected and used to isolate antibiotic producing bacteria (APB). Those isolates (108) were first tested using Cross-streak method against test bacteria. Then, isolates that showed a positive antibacterial effect (12) were tested by antibiotic susceptibility test (AST) of their cell free supernatant (CFS) and their extracellular and intracellular secondary metabolites extraction which gave positive results. Staphylococcus aureus found to be the most sensitive test bacteria with inhibitory zones ranging from 13.5 - 19 mm. Moreover, combinatorial effect of isolates CFS with two organic acids (3% Acetic acid and 0.4 mg/ml Acetylsalicylic acid), two commercial antibiotics (0.016 mg/ml Augmentin and 0.128 mg/ml Doxycycline), and two pure antibiotics (10 mcg/disk Penicillin and 25mcg/disk Carbenicillin) was in vitro evaluated using AST. The combinations of CFS-carbenicillin showed a marked synergistic activity against all test bacteria. The presence of possible antibacterial agents as acetic acid, lactic acid and citric acid in CFS of APB was confirmed by HPLC analysis. Ultimately, in vitro antibacterial study for rhizosphere soil bacteria in this work suggests the possibility of using these bacterial metabolites in clinical infections caused by selected test bacteria, especially when they combine with antibiotics or organic acids.
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Glazunova, Darina, Polina Kuryntseva, Polina Galitskaya und Svetlana Selivanovskaya. „ASSESSMENT OF THE DIVERSITY OF RHIZOSPHERIC CULTIVATED BACTERIA IN WHEAT PLANTS GROWN ON DIFFERENT SOIL TYPES“. In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022v/6.2/s25.11.
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Microbial communities associated with the plant rhizosphere play an important role in carbon sequestration, regulation of nutrient cycling, and the efficient functioning of the ecosystem as a whole. The diversity of microorganisms inhabiting the plant rhizosphere and their complex interactions with the host plant significantly affect the morphology, physiology, growth, development, and health of plants. At the same time, it is known that the soil microbiome diversity is affected by the type of soil, the type of cultivated crop, and the method of tillage. In this study, the abundance and diversity of cultivated bacteria of the rhizosphere microbiome of wheat was assessed. Rhizospheric soil samples were taken from 5 fields with different types of soils (Greyzem, Chernozem, Podzols, Podzoluvisols, Podzoluvisols). Cultivated bacteria from the rhizosphere soil were isolated on meat-peptone and soil agars, and their number was determined. It has been established that the cultivated bacterial rhizobiome was least diverse in wheat plants grown on medium podzolic soil. The MALDI-TOF method was used to identify isolated cultivated isolate species. The genera Achromobacter, Acinetobacter, Bacillus, Microbacterium, Paenibacillus, Pseudomonas, Stenotrophomonas predominated among the isolated bacteria.
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AlBERMANI, Oruba K., Isrra Adnan Auda KHADHIM und Nebras Mohammed SAHI. „ANTIMICROBIAL SUSCEPTIBILITY PATTERN AND THE ANTAGONISTIC EFFECT OF LACTOBACILLUS IN FIGHTING SHIGELLA SPP. ISOLATED FROM DIARRHEIC CHILDREN“. In III.International Scientific Congress of Pure,Appliedand Technological Sciences. Rimar Academy, 2021. http://dx.doi.org/10.47832/minarcongress3-9.
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Background: Shigella is an major source of bacterial gastroenteritis in lack of health awareness in society. The handling of shigellos is mostly requires antibiotics. The using of probiotic of Lactic acid bacteria possess the counter effect against many dangerous bacterial pathogens which associated with gastroenteritis like Shigella spp. Aim: the purpose of this study to estimate the influence of lactic bacteria of the genus Lactobacillus on the population Shigella as a main pathogen involved in gastroenteritis in children. Patients and methods: A total of 50 stool specimens were collected during the period September2019 to January 2020 from diarrheic children patients age range(1-3)years. Standard bacteriological methods were used to isolate, identify, and determine the antimicrobial susceptibility pattern of Shigellaisolates, and we used fresh culture of Lactobacillus 24 h (previously isolated as member of fecal microbiota from healthy person and identified by molecular assay). Then we done centrifugation to obtain supernatant which have test bioactive materials like bacteriocin. These bacteriocin materials subjected to own antibacterial activity against other bacterial pathogen like Shigella spp. By using agar diffusion method . Results: All the 14 Shigella spp. isolates show 100% resistance to nalidixic acid,. cotrimoxazole , and High resistance to ciprofloxacin (85%), and moderate resistant of ampicillin (64%). In agar- well diffusion method indicated the high antagonistic activity of the strains of Lactobacillus 2, 3, and 4 isolated from health GI tract against all Shigella spp., as a result of their activity the total elimination of Shigella within 24 h was observed. conclusion: the Shigella spp. Strains exhibited antibiotic resistant against more one type of antiobiotic The lactobacilii strains tested during this study showed strong antimicrobial activity against Shigella spp. Key words: Shigellos is, Lactobacillus, 16S Rrna.
Berichte der Organisationen zum Thema "Bacteria isolated"
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Sislak, Christine. Novel Thermophilic Bacteria Isolated from Marine Hydrothermal Vents. Portland State University Library, Januar 2000. http://dx.doi.org/10.15760/etd.1485.
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2
Mendoza, Jonathan Alberto, Carolina Mazo, Lina Margarita Conn, Álvaro Rincón Castillo, Daniel Rojas Tapias und Ruth Bonilla Buitrago. Evaluation of phosphate-solubilizing bacteria associated to pastures of Bracharia from acid soils. Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA, 2015. http://dx.doi.org/10.21930/agrosavia.informe.2015.5.
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Rhizobia have been widely known by their capacity to form a symbiotic relationship with legumes and fix atmospheric nitrogen. Recently, however, rhizobia have shown to associate with plants in different botanical families. In this study, we aimed at elucidating the diversity of rhizobia associated to grasses, and determine their capabilities to solubilize phosphate in both lab and greenhouse experiments. Isolation of rhizobia was performed using rhizosphere from Brachiaria brizantha and B. decumbens and a promiscuous legume trap plant (i.e. Vigna unguiculata). Thirty days after inoculation of the trap plant, rhizobia were isolated from nodules using the conventional protocol, classified in basis on their phenotypic features, and molecularly grouped using Amplified Ribosomal DNA Restriction Analysis (ARDRA). Finally, phosphate solubilization assays and greenhouse experiments were carried out on representatives of each ARDRA cluster. The results showed that the diversity of rhizobia varied between both plant species, which suggests that plant exudates significantly determine the composition of the plant microbiome. Surprisingly, most of the isolated associated to B. brizantha rhizosphere exhibited typical attributes of slow-growing rhizobia, whereas rhizobia from B. decumbens displayed a mixed diversity including slow-, intermediate-, and fast-growing rhizobia. Sequencing of 16S rRNA of ARDRA representatives showed that most of the rhizobia isolated from B. brizantha belonged to the Mesorhizobium and Bradyrhizobium genera, while those isolated from B. decumbens were phylogenetically clustered into Rhizobium and Bradyrhizobium. The capability of the isolates to solubilize phosphate was studied using iron and calcium phosphate. We observed that overall Bradyrhizobium exhibited the highest ability to solubilize iron phosphate; by contrast, calcium phosphate was similarly solubilized within representatives of the three genera. In greenhouse experiments, we found that plants inoculated with isolated BT53, BD17 and BD21 exhibited a significantly higher content of phosphorus (p≤0.05). Additionally, dry weight was significantly higher in the treatment inoculated with BT16 isolate (p≤0.05). We conclude that 1) rhizobia is found associated with grasses, 2) plant genotype determines rhizobia diversity 3) rhizobia are able to solubilize phosphorus, and 4) they might be used to promote plant in different plant families. We further believe that further studies will reveal the true role of those old-known legume symbionts in development and growth of other important crops.
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Kapulnik, Yoram, und Donald A. Phillips. Isoflavonoid Regulation of Root Bacteria. United States Department of Agriculture, Januar 1996. http://dx.doi.org/10.32747/1996.7570561.bard.
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The overall objective of this project was to develop a conceptual framework for enhancing root colonization by beneficial bacteria. To accomplish this aim we tested the hypothesis that production and excretion of the plant phytoalexin medicarpin can be used for creation of a special niche along the legume roots, where beneficial microorganism, such as rhizobium, will have a selective advantage. On the Israeli side it was shown that higher medicarpin levels are exuded following the application of Rhizobium meliloti to the rhizosphere but the specific biochemical pathway governing medicarpin production was not induced significantly enough to support a constant production and excretion of this molecule to the rhizosphere. Furthermore, pathogenic bacteria and chemical elicitors were found to induce higher levels of this phytoalexin and it became important to test its natural abundance in field grown plants. On the US side, the occurrence of flavonoids and nucleosides in agricultural soils has been evaluated and biologically significant quantities of these molecules were identified. A more virulent Agrobacterium tumefaciens strain was isolated from alfalfa (Medicago sativa L.) which forms tumors on a wide range of plant species. This isolate contains genes that increase competitive colonization abilities on roots by reducing the accumulation of alfalfa isoflavonoids in the bacterial cells. Following gene tagging efforts the US lab found that mutation in the bacterial efflux pump operons of this isolate reduced its competitive abilities. This results support our original hypothesis that detoxification activity of isoflavenoids molecules, based on bacterial gene(s), is an important selection mechanism in the rhizosphere. In addition, we focused on biotin as a regulatory element in the rhizosphere to support growth of some rhizosphere microorganisms and designed a bacterial gene construct carrying the biotin-binding protein, streptavidin. Expressing this gene in tobacco roots did not affect the biotin level but its expression in alfalfa was lethal. In conclusion, the collaborative combination of basic and applied approaches toward the understanding of rhizosphere activity yielded new knowledge related to the colonization of roots by beneficial microorganisms in the presence of biological active molecules exuded from the plant roots.
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Crocker, Fiona, Mark Fuller und Kayla Clark. Bioaugmentation for enhanced mitigation of explosives in surface soil. Engineer Research and Development Center (U.S.), April 2024. http://dx.doi.org/10.21079/11681/48450.
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Residual munition constituents (MCs) generated from live-fire training exercises persist in soil and can migrate to groundwater, surface waters, and off-range locations. Techniques to mitigate this potential migration are needed. Since the MC hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) can be biodegraded, soil inoculation with RDX-degrading bacteria (i.e., bioaugmentation) was investigated as a means to reduce the migration potential of RDX. Metagenomic studies using contaminated soils have suggested that a greater diversity of bacteria are capable of RDX biodegradation. However, these bacteria remain uncultivated and are potentially a source of novel enzymes and pathways for RDX biodegradation. In situ soil cultivation of a novel soil array was used to isolate the uncultivated bacteria that had been inferred to degrade RDX. Approximately 10.5% of the bacteria isolated from the soil arrays degraded RDX by the aerobic denitration pathway. Of these, 26.5% were possibly novel species of RDX-degrading bacteria, based on 16S rRNA sequence similarity. Both cell encapsulation in hydrogels and coating cells onto granules of polymeric carbon sources were investigated as carrier/delivery approaches for soil inoculation. However, neither of these approaches could confirm that the observed RDX degradation was by the inoculated bacteria.
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Shapira, Roni, Judith Grizzle, Nachman Paster, Mark Pines und Chamindrani Mendis-Handagama. Novel Approach to Mycotoxin Detoxification in Farm Animals Using Probiotics Added to Feed Stuffs. United States Department of Agriculture, Mai 2010. http://dx.doi.org/10.32747/2010.7592115.bard.
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T-2 toxin, a toxic product belongs to the trichothecene mycotoxins, attracts major interest because of its severe detrimental effects on the health of human and farm animals. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The hypothesis of this project was to develop a protecting system using probiotic bacteria that will express trichothecene 3-O-acetyltransferase (Tri101) that convert T-2 to a less toxic intermediate to reduce ingested levels in-situ. The major obstacle that we had faced during the project is the absence of stable and efficient expression vectors in probiotics. Most of the project period was invested to screen and isolate strong promoter to express high amounts of the detoxify enzyme on one hand and to stabilize the expression vector on the other hand. In order to estimate the detoxification capacity of the isolated promoters we had developed two very sensitive bioassays.The first system was based on Saccharomyces cerevisiae cells expressing the green fluorescent protein (GFP). Human liver cells proliferation was used as the second bioassay system.Using both systems we were able to prove actual detoxification on living cells by probiotic bacteria expressing Tri101. The first step was the isolation of already discovered strong promoters from lactic acid bacteria, cloning them downstream the Tri101 gene and transformed vectors to E. coli, a lactic acid bacteria strain Lactococcuslactis MG1363, and a probiotic strain of Lactobacillus casei. All plasmid constructs transformed to L. casei were unstable. The promoter designated lacA found to be the most efficient in reducing T-2 from the growth media of E. coli and L. lactis. A prompter library was generated from L. casei in order to isolate authentic probiotic promoters. Seven promoters were isolated, cloned downstream Tri101, transformed to bacteria and their detoxification capability was compared. One of those prompters, designated P201 showed a relatively high efficiency in detoxification. Sequence analysis of the promoter region of P201 and another promoter, P41, revealed the consensus region recognized by the sigma factor. We further attempted to isolate an inducible, strong promoter by comparing the protein profiles of L. casei grown in the presence of 0.3% bile salt (mimicking intestine conditions). Six spots that were consistently overexpressed in the presence of bile salts were isolated and identified. Their promoter reigns are now under investigation and characterization.
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Cytryn, Eddie, Mark R. Liles und Omer Frenkel. Mining multidrug-resistant desert soil bacteria for biocontrol activity and biologically-active compounds. United States Department of Agriculture, Januar 2014. http://dx.doi.org/10.32747/2014.7598174.bard.
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Control of agro-associated pathogens is becoming increasingly difficult due to increased resistance and mounting restrictions on chemical pesticides and antibiotics. Likewise, in veterinary and human environments, there is increasing resistance of pathogens to currently available antibiotics requiring discovery of novel antibiotic compounds. These drawbacks necessitate discovery and application of microorganisms that can be used as biocontrol agents (BCAs) and the isolation of novel biologically-active compounds. This highly-synergistic one year project implemented an innovative pipeline aimed at detecting BCAs and associated biologically-active compounds, which included: (A) isolation of multidrug-resistant desert soil bacteria and root-associated bacteria from medicinal plants; (B) invitro screening of bacterial isolates against known plant, animal and human pathogens; (C) nextgeneration sequencing of isolates that displayed antagonistic activity against at least one of the model pathogens and (D) in-planta screening of promising BCAs in a model bean-Sclerotiumrolfsii system. The BCA genome data were examined for presence of: i) secondary metabolite encoding genes potentially linked to the anti-pathogenic activity of the isolates; and ii) rhizosphere competence-associated genes, associated with the capacity of microorganisms to successfully inhabit plant roots, and a prerequisite for the success of a soil amended BCA. Altogether, 56 phylogenetically-diverse isolates with bioactivity against bacterial, oomycete and fungal plant pathogens were identified. These strains were sent to Auburn University where bioassays against a panel of animal and human pathogens (including multi-drug resistant pathogenic strains such as A. baumannii 3806) were conducted. Nineteen isolates that showed substantial antagonistic activity against at least one of the screened pathogens were sequenced, assembled and subjected to bioinformatics analyses aimed at identifying secondary metabolite-encoding and rhizosphere competence-associated genes. The genome size of the bacteria ranged from 3.77 to 9.85 Mbp. All of the genomes were characterized by a plethora of secondary metabolite encoding genes including non-ribosomal peptide synthase, polyketidesynthases, lantipeptides, bacteriocins, terpenes and siderophores. While some of these genes were highly similar to documented genes, many were unique and therefore may encode for novel antagonistic compounds. Comparative genomic analysis of root-associated isolates with similar strains not isolated from root environments revealed genes encoding for several rhizospherecompetence- associated traits including urea utilization, chitin degradation, plant cell polymerdegradation, biofilm formation, mechanisms for iron, phosphorus and sulfur acquisition and antibiotic resistance. Our labs are currently writing a continuation of this feasibility study that proposes a unique pipeline for the detection of BCAs and biopesticides that can be used against phytopathogens. It will combine i) metabolomic screening of strains from our collection that contain unique secondary metabolite-encoding genes, in order to isolate novel antimicrobial compounds; ii) model plant-based experiments to assess the antagonistic capacities of selected BCAs toward selected phytopathogens; and iii) an innovative next-generation-sequencing based method to monitor the relative abundance and distribution of selected BCAs in field experiments in order to assess their persistence in natural agro-environments. We believe that this integrated approach will enable development of novel strains and compounds that can be used in large-scale operations.
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Zhou, Ting, Roni Shapira, Peter Pauls, Nachman Paster und Mark Pines. Biological Detoxification of the Mycotoxin Deoxynivalenol (DON) to Improve Safety of Animal Feed and Food. United States Department of Agriculture, Juli 2010. http://dx.doi.org/10.32747/2010.7613885.bard.
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The trichothecene deoxynivalenol (DON, vomitoxin), one of the most common mycotoxin contaminants of grains, is produced by members of the Fusarium genus. DON poses a health risk to consumers and impairs livestock performance because it causes feed refusal, nausea, vomiting, diarrhea, hemolytic effects and cellular injury. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The overall objective of this project was to develop a protecting system using probiotic bacteria that will express trichothecene 3-O-acetyltransferase (Tri101) that convert T-2 to a less toxic intermediate to reduce ingested levels in-situ. The major obstacle that we had faced during the project is the absence of stable and efficient expression vectors in probiotics. Most of the project period was invested to screen and isolate strong promoter to express high amounts of the detoxify enzyme on one hand and to stabilize the expression vector on the other hand. In order to estimate the detoxification capacity of the isolated promoters we had developed two very sensitive bioassays.The first system was based on Saccharomyces cerevisiae cells expressing the green fluorescent protein (GFP). Human liver cells proliferation was used as the second bioassay system.Using both systems we were able to prove actual detoxification on living cells by probiotic bacteria expressing Tri101. The first step was the isolation of already discovered strong promoters from lactic acid bacteria, cloning them downstream the Tri101 gene and transformed vectors to E. coli, a lactic acid bacteria strain Lactococcuslactis MG1363, and a probiotic strain of Lactobacillus casei. All plasmid constructs transformed to L. casei were unstable. The promoter designated lacA found to be the most efficient in reducing T-2 from the growth media of E. coli and L. lactis. A prompter library was generated from L. casei in order to isolate authentic probiotic promoters. Seven promoters were isolated, cloned downstream Tri101, transformed to bacteria and their detoxification capability was compared. One of those prompters, designated P201 showed a relatively high efficiency in detoxification. Sequence analysis of the promoter region of P201 and another promoter, P41, revealed the consensus region recognized by the sigma factor. We further attempted to isolate an inducible, strong promoter by comparing the protein profiles of L. casei grown in the presence of 0.3% bile salt (mimicking intestine conditions). Six spots that were consistently overexpressed in the presence of bile salts were isolated and identified. Their promoter reigns are now under investigation and characterization.
8
Thurston, Alison, Logan Gonzalez, Flora Laurent, Elizabeth Corriveau und Robyn Barbato. Isolation and characterization of bacterial isolates from Alaskan permafrost for synthetic biology applications. Engineer Research and Development Center (U.S.), September 2023. http://dx.doi.org/10.21079/11681/47645.
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Operations in the Artic and other cold regions require technologies that can perform reliably under extreme cold conditions. Permafrost and frozen soils harbor a wide range of microorganisms that have adapted to extremely low temperatures and have unique metabolic capabilities relevant to military operations and that could be exploited to develop biotechnologies optimized for cold environments. Cold-tolerant bacteria (psychrophiles and psychrotrophs) are critical to the development of synthetic biology technologies meant to work in cold environments like the Arctic. Using bacteria isolated from Alaskan permafrost, we applied an experimental pipeline to test the best candidates for use as biological platforms, or chassis, for low-temperature synthetic biology. Since synthetic biology constructs will perform only as well as their chassis, it is critical that circuits expected to perform under extreme cold conditions are housed in chassis that are adapted to those conditions. We identified one permafrost isolate, PTI8, related to Rhodococcus fascians, that is capable of growing from −1°C to at least 25°C and which we experimentally confirmed to uptake and express the broad host range plasmid pBTK519, suggesting PTI8 is a candidate for use as a novel cold-adapted chassis for synthetic biology.
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Kloepper, Joseph W., und Ilan Chet. Endophytic Bacteria of Cotton and Sweet Corn for Providing Growth Promotion and Biological Disease Control. United States Department of Agriculture, Januar 1996. http://dx.doi.org/10.32747/1996.7613039.bard.
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Endophytes were isolated from 16.7% of surface-disinfested seeds and 100% of stems and roots of field-growth plants. Strains from Israel with broad-spectrum in vitro antibiosis were mainly Bacillus spp., and some were chitinolytic. Following dipping of cut cotton roots into suspensions of these strains, endophytes were detected up to 72 days later by isolation and by autoradiograms of 14C-labelled bacteria. Selected endophytes exhibited biological control potential based on significant reductions in disease severity on cotton inoculated with Rhizoctonia solani or Fusarium oxysporum f. sp. vasinfectum as well as control of Sclerotium rolfsii on bean. Neither salicylic acid nor chitinase levels increased in plants as a result of endophytic colonization, suggesting that the observed biocontrol was not accounted for by PR protein production. Some biocontrol endophytes secreted chitinolytic enzymes. Model endophytic strains inoculated into cotton stems via stem injection showed only limited movement within the stem. When introduced into stems at low concentrations, endophytes increased in population density at the injection site. After examining several experimental and semi-practical inoculation systems, seed treatment was selected as an efficient way to reintroduce most endophytes into plants.
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Wackett, Lawrence, Raphi Mandelbaum und Michael Sadowsky. Bacterial Mineralization of Atrazine as a Model for Herbicide Biodegradation: Molecular and Applied Aspects. United States Department of Agriculture, Januar 1999. http://dx.doi.org/10.32747/1999.7695835.bard.
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Atrazine is a broadly used herbicide in agriculture and it was used here as a model to study the biodegradation of herbicides. The bacterium Pseudomonas sp. ADP metabolizes atrazine to carbon dioxide and ammonia and chloride. The genes encoding atrazine catabolism to cyanuric acid were cloned and expressed in Escherichia coli. The genes were designated atzA, atzB and atzC. Each gene was sequenced. The enzyme activities were characterized. AtzA is atrazine chlorohydrolase which takes atrazine to hydroxyatrizine. AtzB is hydroxyatrazine N-ethylaminohydrolase which produces N-isopropylammelide and N-ethylamine. AtzC is N-isopropylammelide N-isopropylaminohydrolase which produces cyanuric acid and N-isopropylamine. Each product was isolated and characterized to confirm their identity by chromatography and mass spectrometry. Sequence analysis indicated that each of the hydrolytic enzymes AtzA, AtzB and AtzC share identity which the aminohydrolase protein superfamily. Atrazine chlorohydrolase was purified to homogeneity. It was shown to have a kcat of 11 s-1 and a KM of 150 uM. It was shown to require a metal ion, either Fe(II), Mn(II) or Co(II), for activity. The atzA, atzB and atzC genes were shown to reside on a broad-host range plasmid in Pseudomonas sp. ADP. Six other recently isolated atrazine-degrading bacteria obtained from Europe and the United States contained homologs to the atz genes identified in Pseudomonas sp. ADP. The identity of the sequences were very high, being greater than 98% in all pairwise comparisons. This indicates that many atrazine-degrading bacteria worldwide metabolize atrazine via a pathway that proceeds through hydroxyatrazine, a metabolite which is non-phytotoxic and non-toxic to mammals. Enzymes were immobilized and used for degradation of atrazine in aqueous phases. The in-depth understanding of the genomics and biochemistry of the atrazine mineralization pathway enabled us to study factors affecting the prevalence of atrazine degradation in various agricultural soils under conservative and new agricultural practices. Moreover, Pseudomonas sp. ADP and/or its enzymes were added to atrazine-contaminated soils, aquifers and industrial wastewater to increase the rate and extent of atrazine biodegradation above that of untreated environments. Our studies enhance the ability to control the fate of regularly introduced pesticides in agriculture, or to reduce the environmental impact of unintentional releases.