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Zeitschriftenartikel zum Thema "Bacillus cereus strains"
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M, Hemalatha, und Subathra Devi C. „OPTIMIZATION OF RIBOFLAVIN PRODUCTION USING BACILLUS CEREUS HDS07: A STRAIN ISOLATED FROM AGARICUS BISPORUS“. Journal of microbiology, biotechnology and food sciences 12, Nr. 5 (01.02.2023): e9066. http://dx.doi.org/10.55251/jmbfs.9066.
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The current study focused on the production and optimization of riboflavin from Bacillus cereus, a strain isolated from Agaricus bisporus. Seven different strains were isolated from Agaricus bisporus and screened for riboflavin production. Among the 7 strains, only 4 strains were identified as riboflavin producers by riboflavin assay medium (RAM). To determine the potency of the strains, selected strains were exposed to roseoflavin – an analogue of riboflavin. The potent strain was identified through morphological, biochemical, and molecular characterization and the strain name was designated as HDS07. Estimation of riboflavin was done by UV Spectrophotometry. Riboflavin production was done in Chemically Defined Medium (CDM) and De Man, Rogosa and Sharpe agar (MRS) using a strain Bacillus cereus HDS07. To enhance riboflavin production, the medium was optimized with different parameters like carbon, nitrogen sources, pH, temperature, and inoculum size. The potent strain HDS07 was identified as Bacillus cereus by 16S rDNA sequencing and NCBI-Gen Bank accession number - MK177597 was obtained. Riboflavin production from Bacillus cereus-HDS07 was more in the MRS medium than that of CDM. It was found to be 2.97 mg/L and 1.8 mg/L correspondingly. The maximum riboflavin (3.48 mg/L) was obtained from Bacillus cereus-HDS07 under the culture conditions; glucose, glycine, pH-6, 30℃, and 3% inoculum size. The current study emphasizes that the isolated potent strain Bacillus cereus-HDS07 from Agaricus bisporus could be used as a starter for the industrial production of riboflavin.
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Han, Cliff S., Gary Xie, Jean F. Challacombe, Michael R. Altherr, Smriti S. Bhotika, David Bruce, Connie S. Campbell et al. „Pathogenomic Sequence Analysis of Bacillus cereus and Bacillus thuringiensis Isolates Closely Related to Bacillus anthracis“. Journal of Bacteriology 188, Nr. 9 (01.05.2006): 3382–90. http://dx.doi.org/10.1128/jb.188.9.3382-3390.2006.
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ABSTRACT Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of B. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.
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Quagliariello, Andrea, Angela Cirigliano und Teresa Rinaldi. „Bacilli in the International Space Station“. Microorganisms 10, Nr. 12 (22.11.2022): 2309. http://dx.doi.org/10.3390/microorganisms10122309.
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Astronauts remote from Earth, not least those who will inhabit the Moon or Mars, are vulnerable to disease due to their reduced immunity, isolation from clinical support, and the disconnect from any buffering capacity provided by the Earth. Here, we explore potential risks for astronaut health, focusing on key aspects of the biology of Bacillus anthracis and other anthrax-like bacilli. We examine aspects of Bacillus cereus group genetics in relation to their evolutionary biology and pathogenicity; a new clade of the Bacillus cereus group, close related to B. anthracis, has colonized the International Space Station (ISS), is still present, and could in theory at least acquire pathogenic plasmids from the other B. cereus group strains. The main finding is that the genomic sequence alignments of the B. cereus group ISS strains revealed a high sequence identity, indicating they originated from the same strain and that a close look to the genetic variations among the strains suggesting they lived, or they are living, in a vegetative form in the ISS enough time to accumulate genetic variations unique for each single strains.
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Daffonchio, Daniele, Noura Raddadi, Maya Merabishvili, Ameur Cherif, Lorenzo Carmagnola, Lorenzo Brusetti, Aurora Rizzi et al. „Strategy for Identification of Bacillus cereus and Bacillus thuringiensis Strains Closely Related to Bacillus anthracis“. Applied and Environmental Microbiology 72, Nr. 2 (Februar 2006): 1295–301. http://dx.doi.org/10.1128/aem.72.2.1295-1301.2006.
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ABSTRACT Bacillus cereus strains that are genetically closely related to B. anthracis can display anthrax-like virulence traits (A. R. Hoffmaster et al., Proc. Natl. Acad. Sci. USA 101:8449-8454, 2004). Hence, approaches that rapidly identify these “near neighbors” are of great interest for the study of B. anthracis virulence mechanisms, as well as to prevent the use of such strains for B. anthracis-based bioweapon development. Here, a strategy is proposed for the identification of near neighbors of B. anthracis based on single nucleotide polymorphisms (SNP) in the 16S-23S rRNA intergenic spacer (ITS) containing tRNA genes, characteristic of B. anthracis. By using restriction site insertion-PCR (RSI-PCR) the presence of two SNP typical of B. anthracis was screened in 126 B. cereus group strains of different origin. Two B. cereus strains and one B. thuringiensis strain showed RSI-PCR profiles identical to that of B. anthracis. The sequencing of the entire ITS containing tRNA genes revealed two of the strains to be identical to B. anthracis. The strict relationship with B. anthracis was confirmed by multilocus sequence typing (MLST) of four other independent loci: cerA, plcR, AC-390, and SG-749. The relationship to B. anthracis of the three strains described by MLST was comparable and even higher to that of four B. cereus strains associated with periodontitis in humans and previously reported as the closest known strains to B. anthracis. SNP in ITS containing tRNA genes combined with RSI-PCR provide a very efficient tool for the identification of strains closely related to B. anthracis.
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CHAVES, JEANE QUINTANILHA, CLARA de FÁTIMA GOMES CAVADOS und ADRIANA MARCOS VIVONI. „Molecular and Toxigenic Characterization of Bacillus cereus and Bacillus thuringiensis Strains Isolated from Commercial Ground Roasted Coffee“. Journal of Food Protection 75, Nr. 3 (01.03.2012): 518–22. http://dx.doi.org/10.4315/0362-028x.jfp-11-325.
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Thirty samples of roasted ground coffee beans from 10 different commercial brands were analyzed to investigate the occurrence and levels of Bacillus cereus and Bacillus thuringiensis strains. Strains were evaluated for their genetic diversity by repetitive element sequence polymorphism PCR (Rep-PCR) and for their toxigenic profiles, i.e., the presence of hblA, hblC, hblD, nheA, nheB, nheC, cytK, ces, and entFM. Survival and multiplication of B. cereus sensu lato in the ready-to-drink coffee was determined to evaluate this beverage as a possible vehicle for B. cereus infection. B. cereus was detected in 17 (56.7%) of the 30 samples, and B. thuringiensis was detected in 8 (26.7%) of the 30 samples. Five samples did not produce any characteristic growth. The most common gene, entFM, was detected in 23 strains (92%). The NHE complex (nheA, nheB, and nheC genes) was found in 19 strains (76%). The HBL complex (hblA, hblC, and hblD) was found in 16 strains (64%). All strains were negative for ces. The cytK gene was found in 16 strains (64%). The computer-assisted cluster analysis of Rep-PCR profiles using a clustering criterion of 80% similarity revealed four main clusters. Cluster 1 was the predominant and comprised three B. thuringiensis strains with 100% similarity, cluster 2 comprised two B. cereus strains (100% similarity), cluster 3 comprised two B. thuringiensis strains (90% similarity), and cluster 4 comprised one B. thuringiensis strain and one B. cereus strain (85% similarity). The cluster analysis of fingerprints generated by Rep-PCR revealed a high genetic diversity among the B. cereus strains, suggesting that the contamination could have originated from different sources. In our experiments, when sugar was added and the beverage was kept in thermic bottles there was a significant increase in B. cereus sensu lato levels, which may increase the risk of food poisoning. These results highlight the need for additional studies on this subject to better evaluate coffee as a food poisoning vehicle.
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MOSSO, Mªs ANGELES, Mª LUISA GARCÍA ARRIBAS, JOSÉ A. CUENA und Mª CARMEN DE LA ROSA. „Enumeration of Bacillus and Bacillus cereus Spores in Food from Spain“. Journal of Food Protection 52, Nr. 3 (01.03.1989): 184–88. http://dx.doi.org/10.4315/0362-028x-52.3.184.
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The Bacillus and B. cereus spore populations of 102 samples of food (salad dressing, dried soups, sweet desserts, milk and milk products, rice dishes, pasta and flour), 93 collected from retail markets of Madrid and 9 from chinese restaurants have been studied. Bacillus spores were detected in 82.4% of the samples, while the incidence of B. cereus spores was 14.7%. In salad dressing and dried soups the contamination rate by species of Bacillus was 100% and also both showed the highest contamination of B. cereus spores (25% and 50% respectively). No samples of rice dishes and pasta exhibited B. cereus spore contamination although these were contaminated by other Bacillus species. All the samples studies showed less than 104 and 105 c.f.u./g or ml B. cereus and Bacillus spores respectively. Twenty-four strains of B. cereus isolated were characterized by morphology and biochemical properties and showed most of the characteristics of the type strain. Enterotoxin, phospholipase C and hemolysin production were present in 13 of the isolated strains showing different degrees of production. The vascular permeability reaction (VPR) was used for determining enterotoxin activity. The enterotoxigenic strains showed a positive VPR; 6 of them caused necrosis and 12 positive mouse lethal tests.
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DE SIANO, TARA, SALLY PADHI, DONALD W. SCHAFFNER und THOMAS J. MONTVILLE. „Growth Characteristics of Virulent Bacillus anthracis and Potential Surrogate Strains“. Journal of Food Protection 69, Nr. 7 (01.07.2006): 1720–23. http://dx.doi.org/10.4315/0362-028x-69.7.1720.
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The objectives of this study were to compare generation and lag times of virulent Bacillus anthracis strains with those of other Bacillus strains, to identify possible surrogates for growth studies, and to determine if the B. cereus module of the U.S. Department of Agriculture Pathogen Modeling Program (PMP) had predictive value for B. anthracis. Growth characteristics of B. anthracis, B. cereus, B. mycoides, and B. subtilis strains in brain heart infusion broth at pH 6.5, 6.0, and 5.5 were determined by absorbance measurements. Growth curves of B. anthracis Sterne and B. cereus strains appeared similar, and the generation times for strain Sterne fell within the PMP's 95% confidence interval for B. cereus. However, the virulent B. anthracis strains Vollum and Pasteur had shorter generation times than the avirulent Sterne strain and most other surrogates and were lower than the PMP's 95% confidence interval for B. cereus. Growth curves of B. cereus ATCC 9818 and B. subtilis ATCC 6633 were more similar to those of virulent B. anthracis strains, but all potential surrogates had significantly different generation times and lag times under some conditions.
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Duc, Le H., Huynh A. Hong, Teresa M. Barbosa, Adriano O. Henriques und Simon M. Cutting. „Characterization of Bacillus Probiotics Available for Human Use“. Applied and Environmental Microbiology 70, Nr. 4 (April 2004): 2161–71. http://dx.doi.org/10.1128/aem.70.4.2161-2171.2004.
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ABSTRACT Bacillus species (Bacillus cereus, Bacillus clausii, Bacillus pumilus) carried in five commercial probiotic products consisting of bacterial spores were characterized for potential attributes (colonization, immunostimulation, and antimicrobial activity) that could account for their claimed probiotic properties. Three B. cereus strains were shown to persist in the mouse gastrointestinal tract for up to 18 days postadministration, demonstrating that these organisms have some ability to colonize. Spores of one B. cereus strain were extremely sensitive to simulated gastric conditions and simulated intestinal fluids. Spores of all strains were immunogenic when they were given orally to mice, but the B. pumilus strain was found to generate particularly high anti-spore immunoglobulin G titers. Spores of B. pumilus and of a laboratory strain of B. subtilis were found to induce the proinflammatory cytokine interleukin-6 in a cultured macrophage cell line, and in vivo, spores of B. pumilus and B. subtilis induced the proinflammatory cytokine tumor necrosis factor alpha and the Th1 cytokine gamma interferon. The B. pumilus strain and one B. cereus strain (B. cereus var. vietnami) were found to produce a bacteriocin-like activity against other Bacillus species. The results that provided evidence of colonization, immunostimulation, and antimicrobial activity support the hypothesis that the organisms have a potential probiotic effect. However, the three B. cereus strains were also found to produce the Hbl and Nhe enterotoxins, which makes them unsafe for human use.
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YANTI, YULMIRA, WARNITA WARNITA, REFLIN REFLIN und HASMIANDY HAMID. „Short Communication: Development of selected PGPR consortium to control Ralstonia syzygii subsp. indonesiensis and promote the growth of tomatoYanti Y, Warnita, Reflin. 2018. Short Communication: Development of selected PGPR consortium to control Ralstoni“. Biodiversitas Journal of Biological Diversity 19, Nr. 6 (09.10.2018): 2073–78. http://dx.doi.org/10.13057/biodiv/d190612.
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Yanti Y, Warnita, Reflin. 2018. Short Communication: Development of selected PGPR consortium to control Ralstonia syzygii subsp. indonesiensis and promote the growth of tomato. Biodiversitas 19: xxxx. A microbial consortium is a group of different species of microorganisms and acts as a community. Combinations of biocontrol strains are expected to have a better result to suppress multiple plant diseases. Our previous research had selected four plant growth promoting rhizobacteria (PGPR) strains from chili (B. pseudomycoides strain NBRC 101232, B. cereus strain CCM 2010, Bacillus toyonensis strain BCT-7112, Serratia nematodiphila strain DZ0503SBS1) and three strains from tomato (Bacillus pseudomycoides strain NBRC 101232, Bacillus toyonensis strain BCT-7112, Bacillus thuringiensis strain ATCC 10792 ) which had ability to promote growth and control Ralstonia syzygii subsp. indonesiensis indigenously. The strains were used in the development of PGPR consortiums to increase their ability for biocontrol agent of Ralstonia syzigii subsp. indonesiensis and promoting the growth of tomato. Results showed that not all strains had good compatibility to grow together. Ten consortiums were developed based on their compatibilities. All consortiums exhibited the capability to reduce bacterial wilt disease development and also promote the growth of tomato. The consortium consisted of Serratia nematodiphila strain DZ0503SBS1, B. cereus strain CCM 2010, Bacillus aryabhattai strain B8W22 and Bacillus cereus strain IAM 12605 resulted in the best ability to reduce disease development and promote growth and yield of tomato.
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McIntyre, Lorraine, Kathryn Bernard, Daniel Beniac, Judith L. Isaac-Renton und David Craig Naseby. „Identification of Bacillus cereus Group Species Associated with Food Poisoning Outbreaks in British Columbia, Canada“. Applied and Environmental Microbiology 74, Nr. 23 (10.10.2008): 7451–53. http://dx.doi.org/10.1128/aem.01284-08.
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ABSTRACT Food poisoning laboratories identify Bacillus cereus using routine methods that may not differentiate all Bacillus cereus group species. We recharacterized Bacillus food-poisoning strains from 39 outbreaks and identified B. cereus in 23 outbreaks, B. thuringiensis in 4, B. mycoides in 1, and mixed strains of Bacillus in 11 outbreaks.
Dissertationen zum Thema "Bacillus cereus strains"
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Leoff, Christine. „Secondary cell wall polysaccharides in Bacillus anthracis and Bacillus cereus strains“. [S.l. : s.n.], 2009.
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Haque, Ahwarul. „Characterisation of Bacillus cereus strains in Bangladeshi rice“. Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272635.
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Altayar, Marei Ahmed. „Aspects of the ecology of Bacillus Cereus strains producing emetic toxin“. Thesis, Glasgow Caledonian University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.443162.
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Abbas, Amina Aicha. „Effet de l’absence d’oxygène sur la capacité de sporulation et les propriétés des spores de Bacillus cereus“. Thesis, Avignon, 2014. http://www.theses.fr/2014AVIG0330/document.
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L’effet de la température et de la composition du milieu en nutriments sur les propriétés des spores (résistance et germination) de B. cereus a été largement étudié contrairement à l'effet de l'anaérobiose. Or, les cellules végétatives de B. cereus peuvent se retrouver dans une grande variété de milieux naturels avec un faible niveau d'oxygène (intestin, sol, lignes de traitement des aliments…) où la sporulation peut avoir lieu. Les spores produites dans ces conditions anaérobies pourraient donc avoir des propriétés particulières. Dans ce travail, un panel de 18 souches de B. cereus appartenant aux groupes phylogénétiques de II à VII a été étudié pour sa capacité à sporuler en anaérobiose dans un milieu de sporulation approprié que nous avons développé (MODS). En anaérobiose, la capacité de sporulation a été plus faible et plus hétérogène qu’en aérobiose. La souche AH187 a produit le niveau de spores le plus important en anaérobiose, elle a donc été choisie pour étudier les propriétés de ces spores. Les spores produites en anaérobiose étaient plus résistantes à la chaleur humide entre 90°C et 100°C, à 1M de NaOH, 1M d'acide nitreux et à la lumière pulsée. Aucune différence dans la résistance à 5 % de peroxyde d'hydrogène ou à 0.25 mM de formaldéhyde, ni aux UV-C, n'a été observée entre les deux conditions. En présence de L- alanine, les spores produites en anaérobiose germaient plus efficacement que celles produites en aérobiose tandis qu’aucune différence dans la germination n’a été observée en présence d'inosine. Aucune différence dans la taille des spores produites dans les deux conditions n’a été observée par microscopie électronique à transmission. Toutefois, les spores obtenues dans des conditions anaérobies avaient un exosporium endommagé ou dans certains cas un exosporium complètement détaché, contrairement aux spores produites dans des conditions aérobies. Afin de comprendre les différences dans la capacité de sporulation de B.cereus entre les 2 conditions, des PCR en temps réel (RT-PCR) ont été utilisées pour étudier l'expression des gènes de l'initiation de la sporulation spo0A, spo0B, spo0F, KinA et kinB. Les cinétiques d'expressions des gènes spo0A, spo0B, spo0F et KinA avaient la même tendance. Ils étaient caractérisés par une expression plus élevée en anaérobiose par rapport à l’aérobiose au début et à la fin de la phase exponentielle de croissance. En outre, l'expression du gène kinB était caractérisée par une augmentation en anaérobiose par rapport à l’aérobiose pour atteindre un pic entre 4 h (milieu de phase exponentielle) et 6 h (début de phase stationnaire) de croissance. Les gènes spo0A, spo0B, spo0F, KinA et kinB sont exprimés de manière différentielle entre l’aérobiose et l’anaérobiose. Ces données pourraient aider à comprendre la différence de capacité de sporulation de B. cereus entre la condition aérobie et anaérobieThe effect of temperature and nutrient composition of the medium on B. cereus spore properties (resistance and germination) has been extensively studied unlike to the effect of anaerobiosis. Nevertheless, B. cereus vegetative cells can be found in a large variety of natural environments with low oxygen level (intestine, soil, food processing line) where sporulation take place. Spores produced in these anaerobic environments could have particular properties. In this work, a panel of B. cereus strains belonging to phylogenetic groups II to VII was studied for their capacity to sporulate in anaerobiosis in an appropriate sporulation medium we developed (MODS). In anaerobiosis, sporulation ability was lower and more heterogeneous than in aerobiosis. The B. cereus AH187 strain produced the highest level of spores in anaerobiosis, it was therefore chosen to study spore properties. Spores produced in anaerobiosis were more resistant to wet heat from 90°C to 100 °C, 1M NaOH, 1M nitrous acid and pulsed light. No difference in resistance to 5 % hydrogen peroxide or 0.25 mM formaldehyde or UV-C was observed between these two conditions. In the presence of L-alanine, spores produced in anaerobiosis germinated more efficiently than spore produced in aerobiosis. No difference in germination was observed with inosine. No difference in the spores size produced in the two conditions was observed by transmission electron microscopy. However, spores obtained under anaerobic conditions had a damaged exosporium, or in some cases a completely detached exosporium, unlike spores produced under aerobic conditions. To understand differences in sporulation ability between both conditions, Real-time reverse transcription-PCR was used to study the expression the expression of sporulation initiation genes spo0A, spo0B, spo0F, kinA and kinB. The kinetics of gene expression spo0A, spo0B, spo0F and kinA had the same trend. They were characterized by a higher expression in anaerobiosis compared to aerobiosis at the beginning and the end of exponential growth phase. Furthermore, kinB gene expression was characterized by an increase in anaerobiosis compared to aerobiosis to achieve a peak between 4 (middle exponential phase) and 6 (early stationary phase) hours of growth. The spo0A, spo0B, spo0F, kinA and kinB genes are differentially expressed between aerobiosis and anaerobiosis. These data may help to understand the difference in B. cereus sporulation capacity between aerobic and anaerobic condition
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He, Minyan, Xiangyang Li, Liang Guo, Susan Miller, Christopher Rensing und Gejiao Wang. „Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1“. BioMed Central, 2010. http://hdl.handle.net/10150/610053.
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BACKGROUND:Chromium is a toxic heavy metal, which primarily exists in two inorganic forms, Cr(VI) and Cr(III). Chromate Cr(VI)] is carcinogenic, mutational, and teratogenic due to its strong oxidizing nature. Biotransformation of Cr(VI) to less-toxic Cr(III) by chromate-resistant and reducing bacteria has offered an ecological and economical option for chromate detoxification and bioremediation. However, knowledge of the genetic determinants for chromate resistance and reduction has been limited so far. Our main aim was to investigate chromate resistance and reduction by Bacillus cereus SJ1, and to further study the underlying mechanisms at the molecular level using the obtained genome sequence.RESULTS:Bacillus cereus SJ1 isolated from chromium-contaminated wastewater of a metal electroplating factory displayed high Cr(VI) resistance with a minimal inhibitory concentration (MIC) of 30 mM when induced with Cr(VI). A complete bacterial reduction of 1 mM Cr(VI) was achieved within 57 h. By genome sequence analysis, a putative chromate transport operon, chrIA1, and two additional chrA genes encoding putative chromate transporters that likely confer chromate resistance were identified. Furthermore, we also found an azoreductase gene azoR and four nitroreductase genes nitR possibly involved in chromate reduction. Using reverse transcription PCR (RT-PCR) technology, it was shown that expression of adjacent genes chrA1 and chrI was induced in response to Cr(VI) but expression of the other two chromate transporter genes chrA2 and chrA3 was constitutive. In contrast, chromate reduction was constitutive in both phenotypic and gene expression analyses. The presence of a resolvase gene upstream of chrIA1, an arsenic resistance operon and a gene encoding Tn7-like transposition proteins ABBCCCD downstream of chrIA1 in B. cereus SJ1 implied the possibility of recent horizontal gene transfer.CONCLUSION:Our results indicate that expression of the chromate transporter gene chrA1 was inducible by Cr(VI) and most likely regulated by the putative transcriptional regulator ChrI. The bacterial Cr(VI)-resistant level was also inducible. The presence of an adjacent arsenic resistance gene cluster nearby the chrIA1 suggested that strong selective pressure by chromium and arsenic could cause bacterial horizontal gene transfer. Such events may favor the survival and increase the resistance level of B. cereus SJ1.
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Drewnowska, Justyna Małgorzata. „Genetic structure of environmental Bacillus cereus sensu lato strains isolated from Northeastern Poland“. Phd thesis, 2016. http://hdl.handle.net/11320/4827.
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Rozdziały (3 załączniki) w postaci publikacji naukowych w czasopismach FEMS Microbiology Ecology (Appendix 1) oraz PLOS ONE (Appendix 2 oraz Appendix 3).Przedstawiciele B. cereus s.l. występują powszechnie w środowisku naturalnym i wywierają ogromny wpływ na zdrowie człowieka, przemysł spożywczy oraz rolnictwo. Te tlenowe laseczki z jednej strony produkują toksyny szkodliwe dla ludzi i zwierząt, ale znane są również z syntezy wtórnych metabolitów degradujących niebezpieczne związki chemiczne lub wspomagających wzrost roślin. Powyższe właściwości były intensywnie badane w odniesieniu do szczepów o szczególnym znaczeniu gospodarczym i medycznym. Tymczasem pokrewieństwo filogenetyczne i podłoże ekologicznej dywersyfikacji szczepów izolowanych z gleby nie jest dostatecznie poznane. Analizowałam strukturę genetyczną 297 szczepów B. cereus s.l. wyizolowanych z gleby (i) Narwiańskiego PN, (ii) Białowieskiego PN, (iii) Biebrzańskiego PN oraz (iv) gospodarstwa rolnego w Jasienówce. Zidentyfikowałam homogeniczną grupę bakterii (i) zdolną do wzrostu w niskiej temperaturze (ekotyp psychrotolerancyjny) oraz (ii) syntetyzującą ciemno-brązowy barwnik (ekotyp melaninowy). Analizy MLST wskazują na istnienie specyficznych genotypów wśród naturalnych populacji B. cereus s.l. Ponadto wykazałam istnienie trzech grup filogenetycznych, obejmujących zmienną liczbę B. cereus, B. thuringiensis i B. mycoides. Jednakże analizy typów sekwencyjnych i kompleksów klonalnych wskazują, iż środowiskowe izolaty B. cereus s.l. nie reprezentują jednego gatunku. Szczegółowe badania genetyczne, fenotypowe oraz biochemiczne środowiskowych szczepów B. cereus s.l., rzuciły nowe światło na ewolucję oraz ekologiczną adaptację tych bakterii.
B. cereus s.l. are widespread in natural environments and have a significant impact on human health, food industry, and agriculture. On one hand, members of this group synthetize various toxins harmful to humans, herbivores and invertebrates. On the other hand, they are also known as producers of various secondary metabolites whereby they degrade pollutants and promote the growth of plants and animals. These aspects have been intensively studied especially with regard to the B. cereus group members with the highest impact on human health and economy. Meanwhile, the phylogenetic relationships and the basis of ecological diversification of soil B. cereus s.l. remains largely undescribed. I investigated the genetic structure of 297 B. cereus s. l. soil isolates from diverse habitats in Northeastern Poland, such as (i) the Narew NP, (ii) the Białowieża NP, (iii) Biebrza NP, and (iv) agricultural land in Jasienowka. I identified homogenous groups of bacteria able to (i) growth in low temperature (psychrotolerant ecotype) and (ii) synthesis a dark pigment (melanotype). The MLST analysis indicates the existence of specific genotypes within the natural B. cereus s.l. populations. Phylogenetic studies revealed three major clades, in which B. cereus, B. thuringiensis and B. mycoides were intermixed. However, analysis of sequence types and clonal complexes indicate that environmental B. cereus s.l. do not represent one species. Detailed genetic, phenotypic and biochemical analyses of the environmental B. cereus s.l. strains shed new light on the evolution and ecological adaptation of these bacteria to specific soil habitats differing in scope of human activity.
Wydział Biologiczno-Chemiczny. Instytut Biologii.
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Leoff, Christine [Verfasser]. „Secondary cell wall polysaccharides in Bacillus anthracis and Bacillus cereus strains / von Christine Leoff“. 2009. http://d-nb.info/993107303/34.
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謝尤敏. „Enterotoxigenic profiles, PCR detection and molecular typing for foodpathogenic bacillus cereus strains in Taiwan“. Thesis, 2001. http://ndltd.ncl.edu.tw/handle/05010536752219501652.
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博士國立中興大學
食品科學系
89
Abstract Bacillus cereus is one of the major foodborne pathogens in Taiwan. The diarrheal type of diseases is attributed to enterotoxins produced during vegetative growth of B. cereus. The B. cereus group comprises B. cereus, B. thuringiensis, B. mycoides and B. anthracis. The virulence properties of B. cereus group cells are of interest. One hundred and one strains of B. cereus group were examined for the presence of four enterotoxin genes: the hemolysin BL (hbl), the non-hemolytic enterotoxin (nhe), the Bacillus cereus enterotoxin T (bceT) and enterotoxin FM (entFM) using polymerase chain reaction (PCR). Different PCR primers were developed for the detection of these genes. Two B. anthracis strains were found to be PCR-negative for these four enterotoxin genes. At least one of the four enterotoxin genes was detected in any of the 89 B. cereus, 7 B. thuringiensis and 3 B. mycoides strains. Thirty of 89 B. cereus strains, all of the 7 B. thuringiensis and 3 B. mycoides strains carried hbl operon genes i.e., hblA, hblC and hblD. Two genes of hblC and hblD were detected in B. cereus strain BCN32, but hblA was not found. The results from the amplification of hblC correlated well with results obtained with the BCET-RPLA kit (Oxoid; Denka Seiken, Japan). Except for two strains (B. cereus strains BCN32 and BCN58), all strains that were positive in PCR amplification using primers L2F/L2b were also positive when tested with the BCET-RPLA kit. The bceT gene was found in 45 of the 101 strains of B. cereus group and entFM in 95 strains. The nhe operon was the most common enterotoxin gene detected in strains examined (97/101; 96%). These results showed that there were 8 enterotoxigenic profiles for the 101 strains of B. cereus group collected. Profile III that carrying the entFM and nhe genes was the most prevalent type (41/101; 41%). Culture supernatants from all strains of B. cereus, B. thuringiensis and B. mycoides were cytotoxic to HEp-2, CHO and Vero cells. Of the three cell lines tested, the HEp-2 cell was less susceptible to the enterotoxin of B. cereus than the CHO and Vero cells. Cell cytotoxicity was detectable only after the cell concentration of B. cereus reached ＞5 × 107 cfu/ml and the Cytotoxicity was about 1/2 ~ 1/8 at the stationary phase. PCR-RFLP analysis and PCR-directed sequencing were performed to examine the heterogeneity of the hbl and nhe genes. The PCR products of hbl genes were found to be heterogeneous by the PCR-RFLP analysis (17/40; 42%). PCR-RFLP analysis also confirmed that there was genetic diversity within the nheA and nheB genes. The sequences different genes of hbl and nhe were found to be highly conserved for different strains. However, most of the base pair substitution occurred at the third base position of the genetic codon and did not affect the amino acid sequence. The hblA, hblC and hblD sequences were used to construct a phylogenetic tree. The strains of B. thuringiensis and B. cereus were closely related species, also B. cereus strain BC13 and B. mycoides strains BMY1, BMY3 were revealed to be closely related species. B. cereus strains were typed by antibiotic susceptibility testing, plasmid profile, RAPD (Randomly Amplified Polymorphic DNA) and PFGE (Pulsed-field gel electrophoresis).Of the 83 B. cereus strains tested, there were 24 antibiogram and 46 plasmid profiles. Plasmid was not founded in 27 B. cereus strains. Digestion with NotI generated 58 PFGE profiles for these 83 B. cereus strains. RAPD with 5 different primers yielded 42 to 55 RAPD patterns. Results obtained from RAPD and PFGE were closely related among the B. cereus strains BCN29 ~ BCN58. Although the RAPD method was rapid and easier to perform, PFGE produced most discriminative and reproducible results.
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Fricker, Martina [Verfasser]. „Development of genotypic and phenotypic methods for the identification and differentiation of hazardous Bacillus cereus group strains / Martina Fricker“. 2007. http://d-nb.info/987922661/34.
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Lin, Gen Yu, und 林亙昱. „Synthesis of biodegradable poly (3-hydroxybutyrate) by a Bacillus cereus strain ISU-02“. Thesis, 2014. http://ndltd.ncl.edu.tw/handle/30478764257151892354.
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碩士義守大學
生物科技學系
102
Global environmental pollution has become an international issue, thus the utilization and application of biodegradable plastics were studied for the prevention of environmental pollution. For the past few years, development of non-toxic biodegradable polymers, were considered as main focus in tissue engineering research field. We isolated microorganisms from the activated sludge, and selected the strains which can produce biodegradable polyester. Using the API 20E system to detect the biochemical characteristics of the isolate, and identify strain it as Bacillus cerues by 16s rDNA sequencing and blast. Phylogenetic tree with other related strain were obtained using bioedit and Mega6. Several experiments were designed to optimize the composition of the culture medium and environmental factors for maximizing polyester production. The results indicated that octanoic acid as carbon source and incubation temperature 30°C and an initial pH 8.0 were optimum for polyester production. Polyester produced from this isolate were identified as Poly(3-hydroxybutyrate) P(3HB) through DSC and NMR.
Buchteile zum Thema "Bacillus cereus strains"
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Drean, Paul, und Edward M. Fox. „Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains“. In Methods in Molecular Biology, 71–83. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2599-5_7.
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2
Gilbert, G. S., J. Handelsman und J. L. Parke. „Role of ammonia and calcium in lysis of zoospores of Phytophthora spp. by Bacillus cereus strain UW85“. In The Rhizosphere and Plant Growth, 300. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3336-4_58.
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Primrose, Sandy B. „Plasmids and Pathogenicity: The Bacillus cereus Complex“. In Microbiology of Infectious Disease, 108–16. Oxford University Press, 2022. http://dx.doi.org/10.1093/oso/9780192863843.003.0014.
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Bacillus cereus, Bacillus anthracis, and Bacillus thuringiensis comprise the Bacillus cereus complex. They are more related to one another than to all other Bacillus species. Bacillus anthracis causes anthrax and there are different forms of the disease: cutaneous, inhalation, and injection. If used as a bioweapon, Bacillus anthracis will cause a fatal inhalation anthrax. The key virulence factors are the plasmid-encoded capsule and components of the oedema toxin and the lethal toxin. Bacillus cereus can cause two types of food poisoning: diarrhoeal disease and emetic disease. Emetic disease is caused by a plasmid-encoded toxin, cereulide. Diarrhoeal disease is caused by three chromosomally encoded toxins. Bacillus thuringiensis strains produce a number of plasmid-encoded toxins that are insecticidal. Different toxins are lethal for different insects. The parent of the Bacillus cereus complex most likely was a diarrhoeal strain of Bacillus cereus because it has no plasmid-borne toxins. This strain then acquired different plasmids to generate the different pathogens in the complex as we know it today.
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van Netten, P., P. van Hoesel und A. van de Moosdijk. „PSYCHROTROPHIC STRAINS OF BACILLUS CEREUS PRODUCING ENTEROTOXIN“. In Food Policy Trends in Europe, 204. Elsevier, 1995. http://dx.doi.org/10.1016/b978-1-85573-284-1.50031-1.
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Urhan, Edip, und H. Yeşim Can. „Bacillus cereus Isolates Obtained from Food Used in Infant and Child Nutrition“. In Current Researches in Health Sciences-III. Özgür Yayınları, 2023. http://dx.doi.org/10.58830/ozgur.pub305.c1247.
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In the present study, a total of 80 sample materials were used (30 powdered baby formulas, 25 jarred baby foods, and 25 cereal-based products). The isolation of Bacillus cereus from the samples was performed with the classical culture technique. The isolates were confirmed as B. cereus with PCR targeting the hemolysin gene. Emetic (ces) and diarrheal toxin genes (nhe, hbl, cytK) were detected by multiplex PCR in confirmed isolates.
As a result of the study, 62.5% (50/80) of the samples were found to be contaminated with B. cereus. B. cereus could not be detected in jarred baby foods. B. cereus was detected at 83.3% (25/30) and 100% (25/25) in powdered baby foods and cereal-based products, respectively. Thirteen (26%) of 50 isolates confirmed by PCR carried at least one gene responsible for toxin synthesis. Only nhe gene was found in three (6%) isolates, and nhe and cytK genes were found together in 10 (20%) isolates. It was found that none of the isolates had ces and hbl genes and all isolates were able to form hemolysis on blood agar and two isolates were psychrotrophic. In conclusion, the presence of enterotoxigenic and psychrotrophic B. cereus strains in powdered baby formulas and cereal-based products may be risky for infants and young children whose immune systems and intestinal microbiota are not fully developed.
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Moummou, Hanane. „Natural Medicine: In-Depth Exploration of Moringa oleifera’s Bioactive Compounds and Antimicrobial Effects“. In The Global Burden of Disease and Risk Factors - Understanding and Management [Working Title]. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.1005046.
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The antimicrobial capabilities of Moringa oleifera have garnered significant scientific attention due to its rich array of bioactive compounds. This chapter provides a comprehensive examination of the antimicrobial activities exhibited by various components of the Moringa oleifera plant, including seeds, leaves, roots, fruits, and flowers. Notably, Moringa seeds, containing potent 4-(alpha-L-rhamanosyloxy) benzyl isothiocyanates, demonstrate strong antimicrobial effects against a broad spectrum of bacterial strains, including Bacillus cereus and Staphylococcus aureus, as well as fungi. Furthermore, lectins within Moringa seeds interact with bacterial membranes, impeding growth and viability. Moringa leaves exhibit pronounced antimicrobial actions against both Gram-positive and Gram-negative bacteria, facilitated by phenolic compounds that disrupt essential bacterial functions. Similarly, Moringa roots demonstrate antibacterial and antifungal properties, attributed to compounds like N-benzylethyl thioformate, presenting promising alternatives to conventional antibiotics. Additionally, Moringa fruits and flowers display significant antimicrobial efficacy, with bioactive compounds such as phenols and flavonoids demonstrating activity against common pathogens like Candida albicans and Escherichia coli. This in-depth analysis underscores the multifaceted antimicrobial potential of Moringa oleifera, highlighting pathways for further research and the development of novel antimicrobial agents and nutraceuticals.
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A. Lawal, Oladipupo, Kehinde O. Amisu, Rebamang A. Mosa, Foluso O. Osunsanmi und Andy R. Opoku. „Melaleuca bracteata var. Revolution Gold (Myrtaceae) Essential Oil: Chemical Composition, Antibacterial, Membrane Damage, Antiplatelet Aggregation and Antiacetylcholinesterase Activities“. In Medicinal Plants - Chemical, Biochemical, and Pharmacological Approaches [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.113238.
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Melaleuca bracteata var. Revolution Gold (a cultivar of Melaleuca bracteata) is an ornamental plant, which has been used in traditional medicine for the treatment of several diseases. Till moment, information is scanty on the biological activities of the essential oil from the plant. The water-distilled essential oil was analyzed by gas chromatography and gas chromatography-mass spectrometry. Antibacterial activity of the oil was evaluated by paper disc diffusion and micro-dilution methods. Cell membrane damage was assay using cytosolic lactate dehydrogenase released method. Platelet aggregation inhibitory activity was separately evaluated on Adenosine diphosphate, collagen, epinephrine and thrombin induced aggregation. Thirteen components representing 95.3% of the total oil were identified from the essential oil. Phenylpropanoids (82.9%) constitute the predominant class of compounds in the oil. On the whole, the oil displayed strong antibacterial action towards Staphylococcus aureus, moderate activity on Bacillus cereus and some strains of Escherichia coli. The lactate dehydrogenase released (0.78–47%) depicted the oil to exhibit low levels of membrane damage. The percentage platelet aggregation inhibition for the four platelet agonists was concentration dependent with thrombin > collagen > ADP > epi-nephrine. The acetylcholinesterase inhibitory activity (9.16%) indicated that the essential oil was not effective against the enzyme.
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Martínez-Álvarez, Juan C., Claudia Castro-Martínez, Melina López-Meyer und Ignacio E. Maldonado-Mendoza. „Enhancing the yield of spores of Bacillus cereus sensu lato strain B25 by evaluating culture media and fermentation parameters“. In Investigaciones Biológicas, Agrícolas y Ambientales de México, 33–44. Pantanal Editora, 2022. http://dx.doi.org/10.46420/9786581460594cap3.
Der volle Inhalt der QuelleKonferenzberichte zum Thema "Bacillus cereus strains"
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Maltseva, S. V., A. S. Yakubovich, E. R. Gritskevitch, I. E. Buchenkov und A. G. Sysa. „ANTAGONISTIC ACTIVITY OF BACTERIA OF THE GENUS BACILLUS ISOLATED FROM SOILS UNDER PROLONGED EXPOSURE TO IONIZING RADIATION IN RELATION TO COLIMORPHOUS BACTERIA“. In SAKHAROV READINGS 2022: ENVIRONMENTAL PROBLEMS OF THE XXI CENTURY. International Sakharov Environmental Institute of Belarusian State University, 2022. http://dx.doi.org/10.46646/sakh-2022-1-299-302.
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This paper presents the results of studies of the antagonistic activity of bacteria of the genus Bacillus (Bacillus subtilis, Bacillus thuringiensis, Bacillus mycoides and Bacillus cereus) under prolonged exposure to ionizing radiation in relation to bacteria of the E. coli group. It was found that bacteria of the genus Bacillus exhibit antagonistic activity of varying degrees of severity. It was found that the bacterial strains Bacillus subtilis, Bacillus thuringiensis and Bacillus mycoides showed a high level of antagonistic activity. Low antagonistic activity was characteristic of Bacillus cereus bacteria.
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Baigonusova, Zh A., A. B. Rysbek und A. A. Kurmanbaev. „Creation of a collection of microorganisms - destructors of organic substances that are promising for bioremediation of technogenic disturbed lands in Kazakhstan“. In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.030.
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The cultural-morphological and biochemical properties of 83 selected strains of organic substance destructor were studied. The analysis of nucleotide sequences of the genome of bacteria Bacillus cereus Fd 2 was performed.
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Sawei, Jelin, und Norrakiah Abdullah Sani. „Prevalence, isolation and characterization of Bacillus cereus strains from rice of local cultivators of Sabah, Sarawak, and Peninsular Malaysia“. In THE 2016 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Author(s), 2016. http://dx.doi.org/10.1063/1.4966767.
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Avdović, Edina, Žiko B. Milanović, Milanka Radulović und Dušan S. Dimić. „ANTIMICROBIAL ACTIVITY OF 3- (1- (3- HYDROXYPHENYL) AMINO) ETHYLIDENE) CHROMAN-2,4-DIONE AND ITS CORRESPONDING PALLADIUM(II) COMPLEX“. In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac, 2021. http://dx.doi.org/10.46793/iccbi21.387a.
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In this manuscript, the in vitro antimicrobial activity of the previously synthesized coumarin derivative 3- (1- (3-hydroxyphenyl) amino) ethylidene) chroman-2,4-dione (L) and its corresponding palladium (II) complex (C) were examined. Their antimicrobial activity was screened against four strains of bacteria Bacillus cereus (ATCC 11778) G+; Staphylococcus aureus (ATCC 13709) G+; Klebsiella pneumoniae (ATCC 27736) G-; and Escherichia coli (ATCC 2592) G-) and three strains of fungi (Aspergillus flavus (ATCC15517); Candida albicans (ATCC 10231); Fusarium oxysporum (ATCC 695) using disc diffusion and microdilution method. The obtained minimum inhibitory concentration (MIC) values by microdilution method for ligand and complex are similar for all tested bacteria and fungi, which means that both compounds have a similar antimicrobial effect. On the other hand, analysis of zone of inhibition (ZI) values for the tested compounds shows that the complex is generally somewhat more active than the ligand.
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Panić, Jovana J., Snežana M. Papović, Teona Teodora V. Borović, Nikolet A. Cako Baganj, Sanja D. Belić, Slobodan B. Gadžurić und Milan B. Vraneš. „The hydration and antimicrobial properties of selected imidazole-based ionic liquids with a homologous series of chloride oxyanions“. In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.124p.
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The aim of this work was to get a detailed insight into the ion’s interactions along with the structure-making/structure-breaking tendency that has been retrieved through the perusal of calculated parameters from volumetric measurements for aqueous solutions of three newly synthesized ionic liquids: 1-butyl-3-methylimidazolium chlorite, 1-butyl-3-methylimidazolium chlorate and 1-butyl-3-methylimidazolium perchlorate. Further, the antimicrobial activity of synthesized and commercial (1-butyl-3-methylimidazolium chloride) ionic liquids on certain strains of bacteria and fungi was obtained. Antimicrobial tests were performed using the in vitro microdilution method against isolated strains of Escherichia coli, Staphylococcus aureus, Bacillus cereus bacteria, and the fungus Candida guilliermondii. This method is a rapid, quantitative method for the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using small amounts of samples (µl) and test compound. Based on the obtained results, the influence of the homologous series of chloride oxyanions on hydration and antimicrobial properties of imidazole-based ionic liquids will be discussed.
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Jawad, Nisreen, Asmat Ahemd und Aminah Abdullah. „Determination of haemolytic and non haemolytic genes profiles of Bacillus cereus strains isolated from food samples by polymerase chain reaction (pcr) technique“. In THE 2017 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Author(s), 2018. http://dx.doi.org/10.1063/1.5027990.
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Obistioiu, Diana, Ileana Cocan, Calin Hulea, Monica Negrea und Ersilia Alexa. „IN VITRO EVALUATION OF BOSWELLIA SPP. ANTI-BIOFILM ACTIVITY“. In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022v/6.2/s25.26.
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Recent years have seen an increase in microbial biofilm resistance, which has led to great challenges for the medical community in terms of treating diseases and the food industry in terms of contamination and the loss of shelf life. The purpose of this work is to test the antimicrobial efficacy against the biofilm formed by the following reference strains: Streptococcus pyogenes (ATCC 19615), Staphylococcus aureus (ATCC 25923), Shigella flexneri (ATCC 12022), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli ( ATCC 25922), Salmonella typhimurium (ATCC 14028), Haemophilus influenzae type B (ATCC 10211), Listeria monocytogenes (ATCC 19114), Bacillus cereus (ATCC 10876) Clostridium perfringens (ATCC 13124), Candida parapsilopsis (ATCC 22019) and Candida albicans ( ATCC 10231) as well as the MBIC evaluation of the essential oil of Boswellia spp. The evaluation was performed by evaluating the loss of microbial mass by measuring the OD by spectrophotometry in accordance with ISO 20776- 1:2019. The statistically evaluated results suggest a very good efficacy against Grampositive bacteria and fungi and a more limited activity against Gram-negative bacteria.
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Obistioiu, Diana, Anca Hulea, Ilinca Imbrea, Iuliana Popescu und Florin Imbrea. „ELETTARIA CARDAMOMUM: CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY. AN IN VITRO STUDY“. In 23rd SGEM International Multidisciplinary Scientific GeoConference 2023. STEF92 Technology, 2023. http://dx.doi.org/10.5593/sgem2023v/6.2/s25.19.
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Recent years have seen an increase in microbial resistance, which has led to great challenges for the medical community in terms of treating diseases and the food industry in terms of contamination and the loss of shelf life. This research aimed to evaluate the chemical composition and antimicrobial activity of the essential oil of Elettaria cardamomum against the following reference strains: Streptococcus pyogenes (ATCC 19615), Staphylococcus aureus (ATCC 25923), Shigella flexneri (ATCC 12022), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli ( ATCC 25922), Salmonella typhimurium (ATCC 14028), Haemophilus influenzae type B (ATCC 10211), Listeria monocytogenes (ATCC 19114), Bacillus cereus (ATCC 10876) Clostridium perfringens (ATCC 13124), Candida parapsilopsis (ATCC 22019) and Candida albicans ( ATCC 10231). The chemical analysis revealed 24 GC-MS- identified compounds using a Shimadzu QP 2010 Plus apparatus (Columbia, SC, USA) equipped with an AT WAX 30 m 0.32 mm 1 ?m capillary column. The antimicrobial activity and MIC evaluation using the microdilution method suggested a higher efficacy against Gram-positive bacteria and fungi than Gram-negative bacteria.
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Floares, Doris, Diana Obistioiu, Anca Hulea, Ersilia Alexa und Isidora Radulov. „THUJA OCCIDENTALIS AND PLATYCLADUS ORIENTALIS ANTIMICROBIAL ACTIVITY“. In 23rd SGEM International Multidisciplinary Scientific GeoConference 2023. STEF92 Technology, 2023. http://dx.doi.org/10.5593/sgem2023v/6.2/s25.57.
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The rise of bacterial resistance to currently employed antibiotics is causing growing concerns for public health. The emergence of highly resistant bacterial strains results in the ineffectiveness of antibiotic treatments against many bacterial infections. As a result, there is an ongoing quest for new antimicrobial agents. This pursuit can take two main directions: one involves the design and synthesis of novel agents, while the other involves exploring natural sources to uncover previously undiscovered antimicrobial compounds. Herbal medications, particularly, have garnered renewed interest due to the perception that they tend to cause fewer adverse reactions when compared to synthetic pharmaceuticals. Moreover, the lower costs of producing plant-based preparations make searching for natural therapeutics appealing. This study aims to assess the antimicrobial properties of Thuja occidentalis (TO) and Platycladus orientalis (PO) against Gram-positive and Gram-negative bacteria using specified reference strains: Streptococcus pyogenes (ATCC 19615), Staphylococcus aureus (ATCC 25923), Listeria monocytogenes (ATCC 19114), Bacillus cereus (ATCC 10876), Clostridium perfringens (ATCC 13124), Shigella flexneri (ATCC 12022), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Salmonella typhimurium (ATCC 14028) and Haemophilus influenzae type B (ATCC 10211) Additionally, we determined the minimum inhibitory concentration (MIC). Following ISO 20776-1:2019 guidelines, we assessed the antimicrobial activity by measuring the reduction in microbial mass through spectrophotometry to determine changes in optical density (OD). Our findings indicate that the TO and PO extracts inhibit Gram-positive bacteria, particularly at the initial concentration tested.
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Alekseev, V. Yu, S. Veselova, D. K. Blagova, E. R. Sarvarova, G. Burkhanova, S. D. Rumyantsev, A. R. Kasimova und I. V. Maksimov. „Recombinant Bacillus subtilis strain deficient in production of surfactin loses ability to induce resistance of wheat plants against aphid Schizaphis graminum (Rond.)“. In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.017.
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An important role of surfactin synthesis by endophytic bacteria in protecting wheat against cereal aphids has been shown to manifest itself in a direct insecticidal effect and an indirect effect through the induction of systemic resistance in plants.
Berichte der Organisationen zum Thema "Bacillus cereus strains"
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Irudayaraj, Joseph, Ze'ev Schmilovitch, Amos Mizrach, Giora Kritzman und Chitrita DebRoy. Rapid detection of food borne pathogens and non-pathogens in fresh produce using FT-IRS and raman spectroscopy. United States Department of Agriculture, Oktober 2004. http://dx.doi.org/10.32747/2004.7587221.bard.
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Rapid detection of pathogens and hazardous elements in fresh fruits and vegetables after harvest requires the use of advanced sensor technology at each step in the farm-to-consumer or farm-to-processing sequence. Fourier-transform infrared (FTIR) spectroscopy and the complementary Raman spectroscopy, an advanced optical technique based on light scattering will be investigated for rapid and on-site assessment of produce safety. Paving the way toward the development of this innovative methodology, specific original objectives were to (1) identify and distinguish different serotypes of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium, and Bacillus cereus by FTIR and Raman spectroscopy, (2) develop spectroscopic fingerprint patterns and detection methodology for fungi such as Aspergillus, Rhizopus, Fusarium, and Penicillium (3) to validate a universal spectroscopic procedure to detect foodborne pathogens and non-pathogens in food systems. The original objectives proposed were very ambitious hence modifications were necessary to fit with the funding. Elaborate experiments were conducted for sensitivity, additionally, testing a wide range of pathogens (more than selected list proposed) was also necessary to demonstrate the robustness of the instruments, most crucially, algorithms for differentiating a specific organism of interest in mixed cultures was conceptualized and validated, and finally neural network and chemometric models were tested on a variety of applications. Food systems tested were apple juice and buffer systems. Pathogens tested include Enterococcus faecium, Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus, Yersinia enterocolitis, Shigella boydii, Staphylococus aureus, Serratiamarcescens, Pseudomonas vulgaris, Vibrio cholerae, Hafniaalvei, Enterobacter cloacae, Enterobacter aerogenes, E. coli (O103, O55, O121, O30 and O26), Aspergillus niger (NRRL 326) and Fusarium verticilliodes (NRRL 13586), Saccharomyces cerevisiae (ATCC 24859), Lactobacillus casei (ATCC 11443), Erwinia carotovora pv. carotovora and Clavibacter michiganense. Sensitivity of the FTIR detection was 103CFU/ml and a clear differentiation was obtained between the different organisms both at the species as well as at the strain level for the tested pathogens. A very crucial step in the direction of analyzing mixed cultures was taken. The vector based algorithm was able to identify a target pathogen of interest in a mixture of up to three organisms. Efforts will be made to extend this to 10-12 key pathogens. The experience gained was very helpful in laying the foundations for extracting the true fingerprint of a specific pathogen irrespective of the background substrate. This is very crucial especially when experimenting with solid samples as well as complex food matrices. Spectroscopic techniques, especially FTIR and Raman methods are being pursued by agencies such as DARPA and Department of Defense to combat homeland security. Through the BARD US-3296-02 feasibility grant, the foundations for detection, sample handling, and the needed algorithms and models were developed. Successive efforts will be made in transferring the methodology to fruit surfaces and to other complex food matrices which can be accomplished with creative sampling methods and experimentation. Even a marginal success in this direction will result in a very significant breakthrough because FTIR and Raman methods, in spite of their limitations are still one of most rapid and nondestructive methods available. Continued interest and efforts in improving the components as well as the refinement of the procedures is bound to result in a significant breakthrough in sensor technology for food safety and biosecurity.