Auswahl der wissenschaftlichen Literatur zum Thema „Azuki bean“

To facilitate the wider use of genetic resources including newly collected cultivated and wild azuki bean germplasm, the genetic diversity of the azuki bean complex, based on 13 simple sequence repeat (SSR) primers, was evaluated and a core collection was developed using 616 accessions originating from 8 Asian countries. Wild germplasm from Japan was highly diverse and represented much of the allelic variation found in cultivated germplasm. The SSR results together with recent archaeobotanical evidence support the view that Japan is one center of domestication of azuki bean, at least for the northeast Asian azuki bean. Cultivated azuki beans from China, Korea, and Japan were the most diverse and were genetically distinct from each other, suggesting a long and relatively isolated history of cultivation in each country. Cultivated azuki beans from eastern Nepal and Bhutan were similar to each other and quite distinct from others. For two primers, most eastern Nepalese and Bhutanese cultivated accessions had null alleles. In addition, wild accessions from the Yangtze River region of China and the Himalayan region had a null allele for one or the other of these primers. Whether the distinct diversity of azuki bean in the Himalayan region is due to introgression or separate domestication events requires further study. In contrast, western Nepalese azuki beans showed an SSR profile similar to that of Chinese azuki beans. The genetic distinctness of cultivated azuki beans from Vietnam has been revealed for the first time. The specific alleles indicate that Vietnamese azuki beans have been cultivated in isolation from Chinese azuki beans for a long time. Wild germplasm from the Himalayan region showed the highest level of variation. Based on the results, Himalayan germplasm could be considered a novel gene source for azuki bean breeding. A comparison with mungbean SSR analysis revealed that the mean gene diversity of cultivated azuki bean (0.74) was much higher than that of cultivated mungbean (0.41). The reduction in gene diversity due to domestication, the domestication bottleneck, in azuki bean is not strong compared with that in mungbean.

A selected set of accessions of components of the azuki bean (Vigna angularis) complex comprising 123 cultivated accessions and 23 wild or weedy accessions from Bhutan, China (including Taiwan), India, Japan, Korea, and Nepal was analyzed using amplified fragment length polymorphism (AFLP) methodology. Using 12 AFLP primer pairs, 580 unambiguous bands were generated, 313 (53.9%) of which were polymorphic among azuki bean accessions. All 580 bands were used to assess phenotypic (band) and genetic (nucleotide) diversity among the 146 azuki bean accessions. The results indicate five major groups of azuki bean germplasm primarily associated with geographic origin of accessions and their status: wild, weedy, or cultivated. These five groups are (i) Himalayan wild, (ii) Nepal–Bhutan cultivated, (iii) Chinese wild, (iv) Taiwan wild – Bhutan cultivated, and (v) northeast Asian accessions. Within the northeast Asian accessions, three subgroups are present. These consist of (v1) Japanese complex – Korean cultivated, (v2) Japanese cultivated, and (v3) Chinese cultivated accessions. The results suggest domestication of azuki bean occurred at least twice, once in the Himalayan region of southern Asia and once in northeast Asia. The remarkable diversity of azuki bean germplasm in the Himalayan region compared with other regions suggests this is a rich source of germplasm for plant breeding. The results suggest there are important gaps in the germplasm collections of azuki bean and its close relatives from various parts of Asia and that specific collecting missions for Vigna germplasm related to azuki bean in the highlands of subtropical Asia are needed.Key words: AFLP markers, Asia, azuki bean, conservation, legume.

Wild relatives of crops have the potential to improve food crops, especially in terms of improving abiotic stress tolerance. Two closely related wild species of the traditional East Asian legume crops, Azuki bean (Vigna angularis), V. riukiuensis “Tojinbaka” and V. nakashimae “Ukushima” were shown to have much higher levels of salt tolerance than azuki beans. To identify the genomic regions responsible for salt tolerance in “Tojinbaka” and “Ukushima”, three interspecific hybrids were developed: (A) azuki bean cultivar “Kyoto Dainagon” × “Tojinbaka”, (B) “Kyoto Dainagon” × “Ukushima” and (C) “Ukushima” × “Tojinbaka”. Linkage maps were developed using SSR or restriction-site-associated DNA markers. There were three QTLs for “percentage of wilt leaves” in populations A, B and C, while populations A and B had three QTLs and population C had two QTLs for “days to wilt”. In population C, four QTLs were detected for Na+ concentration in the primary leaf. Among the F2 individuals in population C, 24% showed higher salt tolerance than both wild parents, suggesting that the salt tolerance of azuki beans can be further improved by combining the QTL alleles of the two wild relatives. The marker information would facilitate the transfer of salt tolerance alleles from “Tojinbaka” and “Ukushima” to azuki beans.

AC Gemco, tested as A235, was developed from a landrace azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] "Martyn Bulk" by pure line selection. It is a medium to full-season-maturing cultvar in southwestern Ontario, and produces a high yield of large red seed. AC Gemco has about 12% more yield, larger seed, and matures about 4 d later than the landrace. Key words: Vigna angularis, azuki bean, red bean, cultivar description

During 2021 and 2022, four experiments were conducted to ascertain the sensitivity of azuki bean to saflufenacil herbicide mixtures, and five experiments were conducted to determine the control of multiple herbicide-resistant (MHR) Canada fleabane with various saflufenacil herbicide mixtures applied preplant (PP) in soybean at various locations in southwestern Ontario, Canada. At 1, 2, 4, and 8 weeks after emergence (WAE), glyphosate + saflufenacil caused 2-5% azuki bean injury. The addition of metribuzin, bromoxynil, halauxifen-methyl, or 2,4-D ester caused 2-7%, 2-4%, 4-9%, and 2-4% azuki bean injury, respectively. Glyphosate + saflufenacil + bromoxynil plus either metribuzin, halauxifen-methyl, or 2,4-D ester caused 3-7%, 5-11%, and 3-6% azuki bean injury, respectively. Saflufenacil mixtures evaluated had no adverse effect on azuki bean stand, biomass m-1, biomass plant-1, height, seed moisture content, or yield. At 4 and 8 weeks after application (WAA), glyphosate + saflufenacil control MHR Canada fleabane 93 and 87%, respectively; there was no improvement in MHR Canada fleabane control with the glyphosate + saflufenacil mixtures evaluated. At 8 WAA, saflufenacil herbicide mixtures evaluated reduced MHR Canada fleabane density 43-95% and biomass 47-96%; differences were not statistically significant. MHR Canada fleabane interference reduced soybean yield 50%; however, reduced MHR Canada fleabane interference with all glyphosate + saflufenacil mixtures evaluated resulted in soybean yield that was similar to the weed-free control. This study concludes that saflufenacil herbicide mixtures evaluated have the potential to be used for the control MHR Canada fleabane in azuki bean.

Five field experiments were conducted in Ontario to determine the tolerance of dry beans to pyraflufen-ethyl (6.7 and 13.4 g ai ha-1), 2,4-D ester (520.3 and 1040.6 g ai ha-1) and pyraflufen-ethyl/2,4-D ester (527 and 1054 g ai ha-1) applied preplant. Pyraflufen-ethyl at 6.7 and 13.4 g ai ha-1 caused < 2% injury in azuki, kidney, small red, and white bean. 2,4-D ester at 520.3 and 1040.6 g ai ha-1 caused up to 4 and 6% injury in azuki bean; up to 5 and 12% injury in kidney bean; up to 7 and 12% injury in small red bean; and up to 5 and 8% injury in white bean, respectively. Pyraflufen-ethyl/2,4-D ester at 527 and 1054 g ai ha-1 caused up to 4 and 6% injury in azuki bean; 5 and 11% injury in kidney bean; 7 and 13% injury in small red bean; and 5 and 10% injury in white bean, respectively. Pyraflufen-ethyl (6.7 and 13.4 g ai ha-1), 2,4-D ester (520.3 and 1040.6 g ai ha-1) or their combination applied preplant caused no adverse effect on dry bean stand, aboveground dry biomass, height, seed moisture content, or yield except for 2,4-D (2X rate) and pyraflufen-ethyl/2,4-D ester (2X rate) which reduced dry bean aboveground biomass as much as 32% and plant height up to 28%. This study concludes that pyraflufen-ethyl (6.7 g ai ha-1), 2,4-D ester (520.3 g ai ha-1), and pyraflufen-ethyl/2,4-D ester (527 g ai ha-1) applied preplant is safe to use for weed management in azuki, kidney, small red, and white bean. However, care must be taken to avoid spray overlaps with 2,4-D ester and pyraflufen-ethyl/2,4-D ester to avoid unacceptable dry bean injury.

Azuki bean (Vigna angularis), belonging to the Fabaceae family, is one of the most traditional crops in East Asia. It is widely consumed in Japan in dishes such as porridge, soups, cakes, pastries, and mixed with rice. Rich in fiber, magnesium, potassium, calcium, and folic acid, azuki beans contain approximately 55% starch. In Brazil, this legume is still relatively unknown, with little information available on its agricultural production. This study aims to analyze and quantify the availability of scientific articles on azuki beans, both at the national and international levels, based on publications from 2012 to 2022. The analysis period it covered the months of August to October 2022. The descriptor “Adzuki Beans” yielded the highest number of publications, with 2021 being the year with the largest volume, totaling 73 articles. In contrast, the descriptor “Feijão Azuki” showed a significantly lower number of articles, with only three publications in 2015. The Web of Science database had the highest number of published articles, totaling 488 publications during the analyzed period.

Azuki bean (Vigna angularis Ohwi & Ohashi) is one of the traditional grain legumes in China. From 2010 to 2013, mosaic and crumpling symptoms on leaves and stunting, all typical symptoms of a viral disease, were observed on cultivars CWA030, CWA221, and JCA002 of azuki bean with incidence rates of 30 to 100% and yield losses of 50 to 95% in the three fields of Changping district, Beijing. To identify the possible viral pathogen(s), 21 symptomatic leaf samples from different cultivars were collected and total RNA was extracted from the samples and subjected to RT-PCR testing with degenerate primers targeting portions of the coding regions of Cucumovirus capsid protein (CP) (1) and Potyvirus NIb (2); these viruses had been reported in azuki bean. Fragments of 940 bp and 350 bp corresponding to Cucumovirus CP and Potyvirus NIb, respectively, were amplified from all the samples collected. Sequencing of the PCR products from nine samples, followed by BLAST analysis, confirmed the presence of Cucumber mosaic virus (CMV) and Bean common mosaic virus (BCMV). All the samples tested were also positive with direct antigen coating (DAC)-ELISA using specific antiserum to CMV or BCMV (Agdia, Elkhart, IN). The CMV CP gene (GenBank Accession No. KJ467817) shared 99% sequence identity with a China CMV isolate (DQ873558). To further characterize the BCMV strain found, fragments of 3,388 bp spanning BCMV NIa, NIb, CP and 3′UTR regions were amplified with another primer set, BCMV-F (5′-AGCAAGTCAATTTACAAGGGACTTC-3′) and BCMV-R (5′-GGAACAACAAACATTGCCGTAGCTAC-3′) from three samples, and three independent clones from each sample were sequenced. Sequence analysis revealed that this segment (KJ467816) shared 98% identity with the BCMV azuki bean strain (U60100). To the best of our knowledge, this is the first report of BCMV, together with CMV, naturally infecting azuki bean in China. Further attention should be paid to this emerging viral disease and measures should be taken to control the spread of BCMV. References: (1) S. K. Choi et al. J. Virol. Methods 83:1345, 1999. (2) L. Zheng et al. Plant Pathol. 59:1345, 2010.

Overweight and obesity are becoming a major public health issue. Pancreatic lipase is a key enzyme to catalyze the hydrolysis of 50-70% of dietary fat in the digestive system. Inhibition of pancreatic lipase activity can block fat absorption in gastrointestinal tract, further control obesity incidence. On the other hand, a-glucosidase is also a key enzyme to hydrolysis polysaccharides and disaccharides into glucose in small intestine. Inhibition of a-glucosidase activity can block digestion and absorption of carbohydrates, further control metabolism disorders such as diabetes and obesity. Food legumes are widely used in people’s life with multiple pharmacological activities. The author extracted total phenolics and saponin components from 13 commonly consumed food legumes produced in China, did a systematic comparative study investigating their inhibitory effects against digestive enzymes (pancreatic lipase and a-glucosidase), and screened adzuki bean (Vigna angularis L.) as the further target bean. The results showed that the different concentrations of total phenolic extract from adzuki bean (0.25, 0.5, 0.75, 1 mg/mL) inhibited hydrolysis of triolein about 24.1, 24.0, 30.7, and 36.3%, respectively, while different concentrations of total saponin extracts from adzuki bean (0.25, 0.5, 0.75, 1 mg/mL) inhibited hydrolysis of triolein about 15.9, 23.5, 30.1, and 29.2%, respectively. On the other hand, phenolic extract of adzuki bean at the concentration from 0.25 to 1 mg/mL exhibited much more than 80% a-glucosidase inhibitory activity, while saponin extract of adzuki bean exhibited 56.4 to 68.7% a-glucosidase inhibitory activity. Based on the results of pancreatic lipase inhibitory activity, at the concentration of 1 mg/mL, phenolics extract and saponin extract of adzuki bean (mean 32.5%) and pinto bean (mean 27%) had the stronger inhibitory effects. Moreover, phenolics extract and saponin extract of adzuki bean (mean 79.25%) and pinto bean (mean 72.85%) also had the stronger inhibitory effects on a-glucosidase activity. In addition, adzuki bean is widely used and has diverse application in foods and drugs. In a word, it indicated that adzuki bean is one of the best target beans to further study anti-obesity and anti-diabetes effects via cell and animal models. Flavonoids and saponins in adzuki bean were obtained and characterized by high performance liquid chromatography with diode array detection and electro spray ionization-tandem multi-stage mass spectrometry in succession. Among 15 compounds identified, four flavonoids (catechin, vitexin-4.-O-glucoside, quercetin-3-O-glucoside, and quercetin-3-O-rutinoside) and six saponins (azukisaponin I, II, III, IV, V, and VI) in adzuki bean were further quantified by external calibration method using high performance liquid chromatography mass spectrometry with the program of time segment and extract ion chromatogram analysis. Animal model is a good way to intuitively evaluate the anti-obesity effect of adzuki bean. So the anti-obesity effects of adzuki bean in mice fed with a high-fat diet was investigated. ICR female mice were fed with a high fat diet administrated orally with different doses of adzuki bean extracts for eight weeks. Total extract, flavonoids and saponins of adzuki bean enhanced lipolysis (166.1%, 175.6%, and 152.6%, respectively). Compared to the final body weight (33.6 g) of the high-fat diet group, oral administration (300 mg/kg per day) of total extract, flavonoids and saponins of adzuki bean significantly reduced the final body weight of mice, and significantly decreased the adipose tissue accumulation. The adzuki bean intervention also significantly reduced the levels of serum triglyceride, total cholesterol, low density lipoprotein-cholesterol, and liver lipid. Adzuki bean demonstrated the anti-obesity effects on mice fed with a high fat diet, such effects may mediated through the inhibitory effects of flavonoids and saponins from adzuki bean on a-glucosidase and pancreatic lipase activities, and lipolysis enhancement effect of active components from adzuki bean. Obesity is characterized biologically at the cellular level by as an increase in the number of preadipocutes and an increase in the size of adipocytes differentiated from preadipocytes in adipose tissue. 3T3-L1 cell model was used to reveal the mechanism of anti-obesity effects of adzuki bean in the present study. The results showed that adzuki bean total extract, adzuki bean flavonoids, adzuki bean saponins, four mono flavonoides (catechin, vitexin-4.-O-glucoside, quercetin-3-O-glucoside, and quercetin-3-O-rutinoside) and six mono saponins (azukisaponin I, II, III, IV, V, and VI) exhibited inhibitory effect of proliferation of 3T3-L1 cells, and the inhibitory rate of proliferation of 3T3-L1 cells was about 20%. Mature adipocytes were stained by Oil Red O and the lipid accumulated exhibited red color, while the preadipocytes could not been stained. The data of the effects of adzuki bean samples on lipid accumulation during the differentiation period of 3T3-L1 cells revealed that azukisaponin II had the highest inhibitory effect (49.72%) with a dose dependent manner from 50 to 500 µg/mL. It indicated that adzuki bean may block the differentiation of 3T3-L1 cells from preadipocytes to mature adipocytes which contribute to anti-obesity effects. Most of adzuki bean samples significantly blocked the triglyceride accumulation and enhanced lipolysis by increseaing the released of glycerol during differentiation of 3T3-L1 cell. Additionally, adzuki bean samples except catechin significantly inhibited glycerol-3-phosphase dehydrogenase activity in 3T3-L1 cells, which indicated that adzuki bean samples had the ability to inhibit triglyceride synthesis. Real-time polymerase chain reaction and Western blot methods were used to investigate the intervention effects of adzuki bean total extract, adzuki bean flavonoids, adzuki bean saponins, quercetin-3-O-rutinoside, and azukisaponin II isolated from adzuki bean on the related gene expressions. These adzuki bean samples inhibited differentiation of 3T3-L1 cell by decreasing the expression of peroxisome proliferator-activated receptor . and CCATT/enhancer-binding protein a (major adipocyte transcription factors) at messenger ribonucleic acid level and protein level in adipocytes. Additional, they inhibited adipogenesis by decreasing the expression of fatty acid synthase, promoting lipolysis by increasing the expression of hormone-sensitive lipase and adipose triglyceride lipase, made energy balance by regulating the expression of leptin, adenosine monophosphate -activated protein kinase, and tumor necrosis factor a, and induced cell apoptosis by increasing the expression of bax and decresing the expression of B-cell lymphoma 2. These findings provide insight into the molecular mechanisums through regulation of the related gene expression in 3T3-L1 adipocytes. It also suggested that adzuki bean had a positive effect in prevention and treatment of adipogenesis-related obesity, and might be a good option of functional foods to control obesity.

碩士
國立中興大學
食品暨應用生物科技學系所
104
Most commercial yogurt are produced form dairy material. However, casein in the milk are commonly considered as allergens which may interfere the iron absorption. In this study, we used physical stimulation to increase GABA content of azuki. Moreover, tartary buckwheat was also adopted as material for introducing functional flavonoids such as rutin and quercetin. Lactobacillus plantarum TWK10 were inoculated to the moromi of buckwheat and azuki to develop a functional vegetable yogurt for providing an alternative choice for vegetarians. The malt solution prepared form ground malt powder was incubated at 35 °C for the activation of intrinsic enzymes. The activated malt solution was then mixed with buckwheat mash at different temperature for serial saccharification processes. Furthermore, the azuki was pretreated by serial soaking, sprouting and freezing processes. The ground frozen azuki was prepared as solution and subsequently gelatinized under 95 °C for 20 minutes. The gelatinized azuki solution was mixed with saccharificated buckwheat solution as buckwheat/azuki moromi. TWK10 was inoculated to the supernatant of azuki/buckwheat moromi for 12 hours fermentation. The pH dropped from 6.00 to 3.92 during the fermentation. In the meanwhile, the microbial density was increased to 12.34 log CFU/mL. Fermented supernatant was mixed with previous separated solid content to form buckwheat/azuki yogurt. The functional components of the buckwheat /azuki yogurt were 44.7 mg GABA/100 mL, 43.417 mg rutin/100 mL and 2.032 mg quercetin/100 mL. No syneresis was observed after one week storage. The results of hedonic tests revealed that 10% added fructose significantly improved the acceptance of yogurt. Further modification may make this functional yogurt more competitive for consumer’s choice.

碩士
嘉南藥理科技大學
生物科技系暨研究所
96
Azuki bean (Vigna angularis (Willd.) Ohwi and Ohashi) is one of the twelve most important grain legumes in the world. In East Asia, the azuki bean is almost applied to food processing extensively. Agrobacterium- mediated gene transfer using azuki bean hypocotyls showed normal transient GUS gene expression, Agrobacterium-induced hypersensitive necrotic reaction in plant cells are seriously problems. Four cultivars of Azuki bean, Kaohsiung NO.6 (KS6), KS7, KS8 and KS9 were tested for plant regeneration by using explants sections from the epicotyls formed the seeds germinating seven days after planting in the dark. It is the Agrobacterium to use different strain with plasmid pCAMBIA 1201 or 1303, including transformation research for EHA105, LBA4404 and KYRT1, try to find out the relevant condition that the suitable azuki bean epicotyls transfer to transformation. The result of the study shows that the Agrobacterium carrying pCAMBIA1201 had higher efficiency than pCAMBIA1303. In gene transfer system of azuki bean, the infection efficiency of EHA105 strain is higher than KYRT1 and LBA4404. Using EHA105- pCAMBIA1201 to infect the explants of azuki bean shows that the KS9 had the biggest GUS gene expression extent, which is up to 67.62%, however, the condition of brownish is the most serious of all. Moreover, the intensity of GUS gene expression of KS6 and KS8 are the strongest, 70.31% and 73.22, respectively. In addition, the accumulation of lignin in KS6 is lower than the other one; the brownish of section cell in KS8 is lower, too.

碩士
國立臺南大學
自然科學教育學系碩士班
94
Legumes are very difficult to transform than other crops because unlike transformation in other organisms, the regeneration system for some legume species is currently not available. Although the most commonly transformation for legumes is based on infection by Agrobacterium tumefaciens, however tissue necrosis and cell death are seriously problems in damage for the success of legume transformation. According to Yamada et al. (2001), we established azuki bean (Vigna angularis Willd) hypocotyls regeneration system for cultivar KS8, the plantlets can be generated within 30-45 days in tissue culture. Agrobacterium-mediated azuki bean hypocotyls gene transfer showed normal transient GUS gene expression, Agrobacterium-induced hypersensitive necrotic reaction in plant cells are seriously problems. Infection with low bacterium density has no apparent improvement in elimination of this tissue necrotic reaction. Histochemical detection for reactive oxygen species (ROS, superoxide, hydrogen peroxide) and lignin accumulation on these necrotic cells revealed that tissue necrosis is affected by ROS production. Hydrogen peroxide production is a biphasic response, the first H2O2 production occurred at 2 h after infection, the second major production happened at 12 h during coclutivation. Antioxidants, such as DTT, GSH, Se, vitamin C, and L-cysteine were incorporated into culture media or bacterium broths in order to reduce tissue necrotic reaction during coclutivation in azuki bean gene transfer. Vitamin C improved callus growth and L-cysteine was the only antioxidant used in our studies that reduced tissue necrosis. However, GUS gene expression is inhibited by using large amount of L-cysteine (>300 mgL-1) although tissue necrosis can be improved dramatically Diphenylene iodonium (DPI) a specific inhibitor for flavoenzyme, such as NADPH oxidase and NADH oxidase was tested for its possible function in improvement Agrobacterium-mediated azuki bean gene transfer. DPI reduced H2O2 accumulation by 32% and superoxide production for 20%, although tissue necrotic reaction was slightly affected by DPI, GUS gene expression was increased. The possible mechanism and useful theory for DPI in Agrobacterium- mediated azuki bean gene transfer are currently under investigation.

碩士
臺南師範學院
自然科學教育學系碩士班
92
Three cultivars of Azuki bean, Kaohsiung NO.8, Pingtung native and KS540 were tested for plant regeneration by using explants sections from the epicotyls formed the seeds germinating seven days after planting in the dark. In the beginning, the explants were placed on MS medium with 10mg l-1 6-benzyladenine in the dark for 2 days. Then, these explants were changed to the MS medium containing 1mg l-1 6-benzyladenine at 25℃ under cool white fluorescent light (16/8 light regime), and adventitious shoots would developed. Roots would be induced on MS medium without plant growth regulators. The efficiency of callus-induced of three genotypes was over 95%. Broadly, the cultivar KS540 has the best regeneration efficiency about 31~42%. However, the regeneration efficiency of epicotyl’s hook of the cultivar Kaohsiung-8 could reach 79.4%, and the minimum forming period was 30-45 days. The high regeneration efficiency and fast growing rate for epicotyls are the advantages to develop Auki bean transformation system. In the transformation of azuki bean by Agrobacterium tumefaciens, co-cultivation was conducted with 6-BA and either acetosyringone (AS), genistein or daidzein. Genistein and daidzein were the important isoflavnoinds in the legume crops. We tested the GUS expression in the early stages after transformation. The GUS expression showed co-cultivation only with 6-BA had better and stable transformation efficiency. Co-cultivation with either acetosyringone, genistein or daidzein, the GUS expression was inhibited and varied widely.

碩士
國立臺灣海洋大學
食品科學系
97
The aims of this study are to investigate the effects of black soy bean or azuki bean infusion on glucose and lipid metabolism in streptozotocin–nicotinamide induced diabetic rats. Forty male Sprague -Dawley (SD) rats were randomly divided into four groups, rats drink water (normal), diabetic rats drink water (DM), diabetic rats drink black soybean infusion (DM + BI), diabetic rats drink azuki bean infusion (DM + AI) for 11 weeks. The results demonstrated that both DM + AI and DM + BI groups could lower the level of glucose, triglyceride and total cholesterol in plasma. In the aspect of carbonhydrate metabolism, DM + AI group could improve glucose tolerance at foruth week and DM + BI group showed the same effect at ninth week. To investigate glucose related metabolism enzyme in liver, hexokinase activity of DM + BI group would be reduced and the glucose-6-phosphatase activity of DM + AI group would be increased. Besides, the DM + AI group could reduce the activity of intestinal lactase and maltase. In the aspect of lipid metabolism, both DM + AI and DM + BI groups could decrease the level of VLDL + LDL- Cholesterol in plasma. Furthermore, DM + BI group also reduced free fatty acid level. On the other hand, DM + AI could decrease the value of TBARS in liver and DM + BI group could decrease the value of TBARS in plasma. In the aspect of oxidative stress, DM + BI group could increase activity of glucose-6-phosphate dehydrogenase, glutathione peroxidase and the GSH/GSSG ratio. According to above- mentioned results, both black soybean and azuki bean infusion had potency to regulate glucose and lipid metabolism in diabetic rats.